CN115671239B

CN115671239B - Anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing cream and preparation method thereof

Info

Publication number: CN115671239B
Application number: CN202211419188.4A
Authority: CN
Inventors: 孙淑萍; 李胜利; 杜云艳; 檀圆圆; 李安琪; 孙琪; 王梁; 朱恩泽; 侯志远; 谢先进; 陈春敬; 孙智玮; 夏荣平; 陈彩云
Original assignee: Wannan Medical College
Current assignee: Wannan Medical College
Priority date: 2022-11-14
Filing date: 2022-11-14
Publication date: 2024-04-16
Anticipated expiration: 2042-11-14
Also published as: CN115671239A

Abstract

The invention provides an anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing cream and a preparation method thereof, wherein the ingredients comprise: zedoary turmeric oil, rose hip oil, calendula soaking oil, aloe oil, evening primrose oil, shea butter, strawberry seed oil, emu oil, hydrogenated lecithin S-10, simple multifunctional emulsifier EMT-10, sibirch 305, distilled water, cherry extract, sodium hyaluronate solution, total hexose polysaccharide, boric acid, trehalose, berberine, jojoba gum, herba Sedi Aizoon extract, spina Gleditsiae extract, paederia scandens extract, watermelon peel extract, red bayberry extract, sodium alginate, ice crystal former AVC, amino acid thickener DOE-120T, U cellulose thickener, carbomer 941, essence, 30% triethanolamine solution, 1, 2-propanediol, and ethylparaben. Compared with the prior art, the invention extracts natural components from various medicines, and can resist inflammation, detumescence, inhibit bacteria, relieve itching, prevent cracking and repair.

Description

Anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing cream and preparation method thereof

Technical Field

The invention belongs to the field of traditional Chinese medicines, and in particular relates to an anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly and a preparation method thereof.

Background

Chilblain is a common skin disease, which is manifested by repeated erythema and swelling injury of local skin, and has slow disease course, self-healing after warming, but easy recurrence. Chilblain is frequently found in winter, and is frequently found on children, women and people with poor peripheral blood circulation, and is well found on fingers, backs of hands, faces, auricles, toes, edges of feet, heels and the like, and is often distributed on two sides. Common lesions are oedema erythema with localized bloody dark mauve mounds, surface tension, bright red edge margin, and local compression can fade, with gradual recovery of red after decompression. In severe cases blisters may develop and break to form erosion or ulcers, with pigmentation or atrophic scars remaining after healing. When chilblain occurs, itching is obvious, and the pain is aggravated after heat and ulceration. Cold is the main cause of chilblain, and chilblain can be caused by factors such as lower immunity, excessive sweat and dampness of hands and feet, long-term working at low temperature, and the like.

At present, some chilblain pastes are available on the market, most of the products are mainly prepared from camphor, menthol, urea, glycerin and the like, and the chilblain pastes have the effect of relieving chilblain symptoms to different degrees, but have slower curative effect, unsatisfactory effect and are easy to relapse after the chilblain is healed.

Disclosure of Invention

The invention aims to provide the anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing cream and the preparation method thereof, which contain various vegetable oil and plant extract components, can inhibit bacteria, relieve itching and repair damaged skin, has quick curative effect and good effect on treating chilblain, is not easy to recur after the chilblain is healed, can improve the immunity of organisms, prevent skin from being frosted, and enable the skin to be moist and glossy.

The specific technical scheme of the invention is as follows:

An anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing cream comprises the following components in parts by weight:

The calendula soaking oil is prepared by the following method:

Weighing appropriate amount of calendula flower petals, adding olive oil with 12-20 times of calendula flower petal mass, extracting at ultrasonic frequency of 20000-40000Hz for 0.5-1.5 hr, soaking at room temperature for 7-15 days, and filtering to obtain calendula soaking oil.

The cherry extract is prepared by the following steps:

Removing the pit of fresh cherry, weighing a proper amount of pulp, putting into a juicer, adding distilled water 3-5 times of the cherry pulp, squeezing juice for 1.5-2.0min in a juicing mode, filtering to obtain filtrate, and performing suction filtration, wherein the volume of the filtrate is 2-3 times of the cherry pulp mL/g, thus obtaining cherry squeezing liquid for later use.

The volume of the filtrate is 2-3 times of the mass of cherry pulp, namely the volume of cherry extract obtained after each g of cherry pulp is extracted by the method is 2-3mL.

The watermelon peel squeezing liquid is prepared by the following steps:

Weighing a proper amount of chopped fresh watermelon peel, putting into a juicer, adding distilled water 3-5 times of the watermelon peel, squeezing juice for 1.5-2.0min in a juice squeezing mode, filtering to obtain filtrate, and performing suction filtration to obtain filtrate with volume of 2-3 times of the watermelon peel mass mL/g to obtain watermelon peel squeezing liquid for later use.

The aizoon stonecrop herb extract is prepared by the following method:

Weighing appropriate amount of coarse powder of radix Notoginseng, and extracting under reflux for 3 times: adding 15-18 times of water into the coarse powder of herba Sedi Aizoon, soaking for 0.25-0.75 hr, and extracting for 0.5-1 hr; adding water with the mass of 10-14 times of that of the coarse powder of the aizoon stonecrop herb for 2 nd time, and extracting for 0.5-1h; adding water with the mass of 8-12 times of that of the coarse powder of the aizoon stonecrop herb for 3 times, and extracting for 0.5-1h; and filtering each time, combining the filtrates, carrying out suction filtration, and concentrating the filtrate volume to 3-7 times of the mass of the aizoon stonecrop coarse powder to obtain aizoon stonecrop extract for later use.

The concentration of the filtrate volume to 3-7 times of the mass of the aizoon stonecrop herb coarse powder is that the volume of aizoon stonecrop herb extract obtained after each g of aizoon stonecrop herb coarse powder is extracted by the method is 3-7mL.

The spina gleditsiae extracting solution is prepared by the following method:

Weighing appropriate amount of spina Gleditsiae coarse powder, and extracting under reflux for 3 times: adding 70% ethanol solution 15-18 times of the coarse powder of spina Gleditsiae, soaking for 0.25-0.75 hr, and extracting for 1.5-2.0 hr; adding 70% ethanol solution with the weight of 9-12 times of that of the spina gleditsiae coarse powder for 2 nd time, and extracting for 1.0-1.5h; adding 70% ethanol solution with the weight of 8-10 times of that of the spina gleditsiae coarse powder for 3 times, and extracting for 0.5-1.0h; and filtering each time, combining the filtrates, carrying out suction filtration, and concentrating the filtrate volume to 3-9 times of the mass of the spina gleditsiae coarse powder to obtain the spina gleditsiae extracting solution for later use.

The preparation method of the Paederia scandens white extract comprises the following steps:

Weighing appropriate amount of Paederia scandens, and extracting under reflux for 3 times: adding 15-18 times of water for soaking for 5-15min, and extracting for 1.5-2.0 hr; adding 10-14 times of water for the 2 nd time, and extracting for 1.0-1.5h; adding water with the mass of 8-10 times of that of the white chicken in the 3 rd time, and extracting for 0.5-1.0h; and filtering each time, combining the filtrates, carrying out suction filtration, and concentrating the filtrate volume to 3-6 times of the quality of the white of the chicken, thereby obtaining the white of the chicken extract for later use.

The waxberry extract is prepared by the following method:

Weighing a proper amount of waxberry, and extracting for 3 times by heating and reflux: adding water 15-18 times of the weight of the waxberry for 1 st time, and extracting for 1.5-2.0h; adding 10-15 times of water to the waxberry for 2 times, and extracting for 1.0-1.5h; adding water with the mass of 8-10 times of that of the waxberry for 3 times, and extracting for 0.5-1.0h; and filtering each time, combining the filtrates, carrying out suction filtration, and concentrating the filtrate volume to 3-5 times of mL/g of the waxberry mass to obtain the waxberry extract for later use.

The total polysaccharide is prepared by the following steps:

Weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding water 15-18 times of the mass of the coarse powder of the radix stephaniae tetrandrae for 15-45min, and extracting for 1.0-1.6h; adding water with the weight 9-13 times of that of the coarse powder of the radix stephaniae tetrandrae for 2 nd time, and extracting for 0.8-1.6h; adding water with the mass of 8-12 times of that of the coarse powder of the radix stephaniae tetrandrae for 3 rd time, and extracting for 0.4-1.2h; filtering each time, mixing filtrates, centrifuging at 4deg.C and 8000r/min for 10-25min, and concentrating clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder; and precipitating with 95% ethanol solution at a volume ratio of 1:7-1:2, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, oven drying the filter cake, pulverizing, and sieving with 80 mesh sieve to obtain HEJI total polysaccharide. The total polysaccharide content was determined by phenol-concentrated sulfuric acid assay with a total polysaccharide yield of about 8.8%.

The essence is one or more selected from jasmine essence, lemon essence, lavender essence, rose essence, lily essence, green melon essence and osmanthus essence.

The invention provides a preparation method of anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing paste, which comprises the following steps:

a) Weighing 1.0-3.0g of amino acid thickener DOE-120T, adding into 10.0-50.0mL of distilled water, stirring, mixing, placing into a water bath kettle with the temperature of 60-80 ℃ heated in advance, continuously stirring for 5-20min, taking out, standing at normal temperature for 12-24h, and fully swelling to obtain amino acid thickener DOE-120T gel solution for later use;

b) Weighing 0.3-0.7g of U30 cellulose thickener, adding into 30.0-70.0mL of distilled water, stirring, mixing, standing at normal temperature for 12-24h, and fully swelling to obtain U30 cellulose thickener gel solution for later use;

c) Weighing 0.3-1.5g of sodium alginate, adding into 25.0-50.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 60-80 ℃ heated in advance, stirring continuously for 5-20min, taking out, standing for 12-24h at normal temperature, and fully swelling to obtain uniform and transparent sodium alginate gel solution for later use;

d) Weighing 0.05-0.20g of ice crystal forming agent AVC, adding into 10.0-50.0mL of distilled water, stirring, mixing uniformly, placing into a water bath kettle with the temperature of 60-80 ℃ heated in advance, continuing stirring for 5-20min, taking out, standing for 12-24h at normal temperature, and obtaining uniform transparent ice crystal forming agent AVC gel liquid for standby after the ice crystal forming agent AVC fully swells;

e) Weighing 0.5-1.0g of carbomer 941, adding into 40.0-70.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 60-80 ℃ heated in advance, continuing stirring for 5-20min, taking out, standing for 12-24h at normal temperature, and obtaining uniform and transparent carbomer 941 gel solution after full swelling;

f) Weighing 0.3-1.2g of zedoary turmeric oil, 0.6-1.1g of rose hip oil, 0.4-1.3g of calendula soaking oil, 0.5-1.5g of aloe oil, 0.2-1.0g of evening primrose oil, 0.8-1.7g of shea butter, 0.4-1.3g of strawberry seed oil, 0.5-1.5g of emu oil and 0.1-2.0g of hydrogenated lecithin S, mixing and stirring, heating and melting in a water bath kettle with the temperature of 70-90 ℃ heated in advance, and uniformly stirring after melting to serve as an oil phase for standby;

g) Weighing 0.1-1.1g of simple multifunctional emulsifier EMT and 0.1-1.2g of Sibiricarb 305, adding 10.0-15.0mL of distilled water, stirring to uniformly mix, heating in a water bath kettle with the temperature of 70-90 ℃ which is heated in advance, and uniformly stirring to serve as a water phase for later use;

h) Adding the water phase into the oil phase in a trickle mode when the oil phase and the water phase reach the same temperature, and stirring towards the same direction while adding to obtain an emulsion matrix;

i) 0.1-1.0mL of cherry extract, 0.5-1.2mL of aizoon stonecrop herb extract, 0.1-1.3mL of spina gleditsiae extract, 0.3-1.0mL of Chinese fevervine root extract, 0.1-1.5mL of watermelon peel extract, 0.5-1.3mL of waxberry extract, 0.1-0.3g of boric acid, 0.1-1.0g of luba glue, 0.001-0.005g of berberine, 0.1-0.3g of trehalose and 0.05-0.15g of total hexose polysaccharide are taken, stirred and dissolved, and uniformly mixed to obtain solution A for standby;

j) Weighing 0.34-0.86g of ethylparaben, adding 1.0-4.0mL of 1, 2-propanediol, stirring and dissolving to obtain antiseptic solution;

k) Adding the prepared solution A into emulsion matrix, stirring and mixing uniformly, adding 1.0-5.0mL of 1% sodium hyaluronate solution, 1.0-120T gel solution of amino acid thickener, U30 cellulose thickener gel solution, sodium alginate gel solution, ice crystal forming agent AVC gel solution, carbomer 941 gel solution, 0.5-1.0mL of 30% triethanolamine solution, and 0.05-0.30mL of preservative solution and essence, stirring and mixing uniformly to obtain the anti-inflammatory, antibacterial, antipruritic, anti-cracking and repairing jelly.

In the preparation method of the invention, the steps a), b), c), d) and e) are five gel thickeners; step f) preparing an oil phase; step g) preparing an aqueous phase; step h) preparing an emulsion matrix by adopting a hot-preparation method; step i) mainly comprises mixing natural functional components; step j) preparation of a preservative; k) The emulsion matrix is added with natural functional components, and is thickened by amino acid thickener DOE-120T gel liquid, U30 cellulose thickener gel liquid, sodium alginate gel liquid, ice crystal forming agent AVC gel liquid and carbomer 941 gel liquid, and 1% sodium hyaluronate solution, 30% triethanolamine solution, preservative solution and essence are added, so that the frozen healing paste has the advantages of fine and uniform texture, proper viscosity, good spreadability, fragrant smell, obvious absorption effect, good moisturizing performance and the like, can inhibit bacterial infection, reduce swelling, deeply moisten damaged skin due to chilblain, has extremely strong healing efficacy, can relieve itching caused by skin chilblain inflammation, and has luster, smoothness and fineness.

The design ideas of various components in the invention are as follows:

The zedoary turmeric oil has the main effects of promoting qi and blood circulation, detumescence and relieving pain, enhancing the immunity of human body, and is mainly used for treating traumatic injury, cold limbs, fire burn, mosquito bite, insect bite, etc. The zedoary turmeric oil has the efficacy and effect mainly aiming at skin infection, has excellent effect on candida, white fungus and other fungi types of diseases, has good synergistic killing effect on bacteria, fungi, viruses and other microorganisms, and can promote the healing of pathological tissues. The effect is obvious, the using method is simple, and the side effect is almost avoided.

The rose hip oil contains a large amount of vitamin E and various minerals, and can play a role in activating collaterals and strengthening tissues. It can promote the activity of epidermis cells to raise the regeneration capacity of skin, and has excellent effect in repairing and healing damaged skin caused by chilblain, and can shrink pores and eliminate red swelling and red spot. Besides, the rose hip oil can lighten skin pigment, effectively reduce striae atrophicae, reproduce uniform tone and restore skin to be tender and white. The rose hip oil also can repair skin, lighten scars, is easy to permeate into dermis, has the effects of moisturizing the skin, and promoting the skin to be tender, soft and whitening.

The calendula has the effects of sterilizing, diminishing inflammation and natural antioxidation, can effectively relieve and repair skin which is easy to be red and swollen and sensitive, and has better assisting effect on preventing and treating skin infection. The calendula oil can effectively relieve eczema and skin itching, accelerate the healing of sores, resist skin allergy, improve immunity and gently moisten skin. It can relieve acne, moisten dry skin, and treat venomous snake bite. In addition, calendula is rich in various vitamins, especially vitamin A and vitamin C, and can prevent pigmentation, improve skin luster and elasticity, slow aging, and prevent skin relaxation, wrinkle, etc. The olive oil is rich in unsaturated fatty acid and various vitamins, is easy to be absorbed by skin, is fresh and natural, and has no greasy feeling. Polyphenols contained in olive oil have antioxidant effect. It can effectively avoid the phenomena of color spots, wrinkles and the like caused by cell aging due to fat oxidation. The olive oil soaking calendula can promote the absorption of skin to the functional components in the calendula, thereby playing a synergistic effect of repairing and moistening.

The aloe oil is natural saponin, has powerful cleaning and antibacterial effects, can effectively diminish inflammation, relieve swelling, relieve itching and pain, and has direct beautifying effects of moistening, moisturizing, whitening, removing freckle, resisting aging, preventing allergy and the like. The aloe oil can inhibit the proliferation of pathogenic microorganisms and bacteria on skin surface, effectively prevent skin diseases, balance and inhibit sebum secretion, and promote wound healing. The aloe oil contains natural gel substance, namely water-retaining lignin, has strong permeability, and can help nutrients to permeate into skin. The beta-carotene contained in the beta-carotene contains a plurality of conjugated double bonds, is a good antioxidant, and can quench free radicals and prevent the generation of the free radicals. Thus, aloe vera is effective in inhibiting free radical-induced lipid peroxidation.

The evening primrose oil is mild and not irritative, fresh and not greasy, is a better moisturizing oil, is suitable for dry aged or cracked skin, can effectively improve eczema and psoriasis, promote wound healing, lighten whelks and black spots, and prevent skin from being cracked and aged. The most main active ingredients are unsaturated fatty acid, which can prevent pore blockage and nourish skin. The essential fatty acid such as linoleic acid or linolenic acid can relieve symptoms of pain and itching caused by skin dryness and even inflammation. In addition, evening primrose oil also helps regulate the production of hormones, thereby preventing the formation of acne.

The shea butter contains a large amount of nutrients, can promote the regeneration of epidermal cells, endow the skin with nutrition effect, and is easy to be absorbed by the skin. In addition, the shea butter contains triglyceride, and can form a protective layer on the skin surface to prevent water evaporation and play a role in moisturizing. In addition, the shea butter can promote the growth of collagen and make skin smooth and fine and wrinkles reduced after long-term use.

The strawberry seed oil is a natural soothing emollient, has good skin-friendly and moisturizing effects, can effectively enhance the smoothness and tenderness of skin, helps to reduce the water loss of skin and increases the elasticity. The strawberry seeds can also effectively promote the proliferation of keratinocytes, improve skin inflammation, regulate skin barrier function and increase the release amount of hyaluronic acid of cells, thereby achieving the moisturizing effect. The strawberry seed oil also contains super antioxidant ellagic acid, which can remove in vivo carcinogenic toxin and improve immunity.

Emu oil has effects of preventing wrinkle, eliminating acne and speckle, and also has antiinflammatory effect. The deep penetrating ability of emu oil promotes healing of burns, eczema, dermatitis and other skin disorders. In addition, emu oil can also relieve pain, has the effects of diminishing inflammation and easing pain, can give skin sufficient moisture, promote skin luster and smoothness, has the effect of moisturizing the skin, prevents the skin from being hurt by the external environment, and arouses cells in the skin again. It contains up to 70% of unsaturated fatty acids, which are the pure natural components closest to the skin fat of the human body and are most suitable for absorption by the human body.

Cherry has warm nature, and has effects of promoting sweat and eruption, and removing toxic substances, and can be used for treating light and severe cold injury and burn. The cherry juice is squeezed out and is frequently smeared on a wound part, so that pain can be immediately relieved, wound infection can be avoided, and the effects of astringing, relieving pain and preventing foaming and suppuration at the wound are achieved. The cherry juice can be used for smearing on face, wrinkle and chilblain, and can make face skin ruddy and tender, remove wrinkle and speckle, and can be used for fading intractable speckle such as facial freckle. Cherry contains abundant anthocyanin, anthocyanidin, vitamin E and the like, and the nutrient elements are effective antioxidants and can treat skin dryness.

Herba Sedi Aizoon has flat nature and sweet taste, has effects of promoting blood circulation, stopping bleeding, promoting diuresis, relieving swelling, removing toxic substance, etc., and can be used for treating traumatic injury, traumatic hemorrhage, burn, scald, sore, furuncle, carbuncle, etc. The aizoon stonecrop herb alcohol extract has an in vitro antibacterial effect and has an inhibition effect on staphylococcus aureus, surface staphylococcus and micrococcus. Meanwhile, the water-soluble extract of the aizoon stonecrop herb has the function of shortening the coagulation time and the bleeding time, so that the chilblain can be effectively treated by adding the water extract into the product. The ursolic acid contained in the aizoon stonecrop herb has various biological effects of sedation, anti-inflammatory, antibiosis and the like, and also has obvious antioxidation function. The arbutin can reduce melanin generation by inhibiting the activity of melanin-producing enzyme tyrosinase, and accelerate melanin decomposition and excretion by combining with tyrosinase, thereby reducing skin pigmentation and removing scar.

The Chinese honeylocust spine, pungent and warm, can be used for treating skin diseases such as pyocutaneous disease, skin rash, eczema and the like, has a certain antiallergic effect, and has no irritation to skin. It can inhibit or kill various gram positive and negative bacteria, and has inhibitory effect on Staphylococcus aureus. The spina gleditsiae has better curative effect on carbuncles and toxic swelling, and is generally indicated for the unoccupied pus and the unoccupied pus can cause the unoccupied pus to quickly collapse. The spina gleditsiae contains abundant insoluble or water-insoluble flavonoid compounds, and the effective components of the spina gleditsiae can be fully leached out by alcohol extraction, so that the spina gleditsiae can be used for resolving liver toxicity and resisting fungi. In addition, the spina gleditsiae has remarkable anti-inflammatory effect, has the effect of promoting and regulating the immune system, can improve the immunity of organisms, and has the effect of promoting the growth of skin cells.

The white chicken has cool nature and is favorable for expelling water and heat, dispelling wind and removing toxicity, promoting blood circulation and relieving pain, and the like. The chicken is stir-fried with white color, then added with water and stirred uniformly, and is applied to the muscle injury part, thus obviously relieving symptoms. It has effects in clearing away heat and toxic materials, inhibiting bacteria growth, removing toxic substances, activating skin, improving skin quality, and relieving inflammation. Boiling herba Paederiae with water, and applying onto hands to treat chilblain.

The watermelon peel has the effect of promoting wound healing, and can be cut into slices for incised wounds, stabbed wounds and the like, and can treat wound bleeding by being applied to the wounds, so that the effects of astringing and diminishing inflammation and promoting wound healing are achieved. Watermelon Pi Fu contains multiple vitamins, can eliminate skin itch, effectively remove free radicals, and has a certain effect on deferring aging. The watermelon peel is rich in vitamin C, is water-soluble vitamin, reduces skin melanin pigmentation, and makes skin become more white.

The fresh waxberry has rich potassium content, and the potassium element has prominent effects in metabolism and other aspects, so the waxberry also has the effects of activating blood and the like. The waxberry can inhibit the dendritic extension of melanocytes in human epidermal cells, and has whitening effect. Myrica rubra bark extract has effects of scavenging free radicals in vivo and resisting oxidation. The anthocyanin and vitamin C which are rich in the water extract have good antioxidation function, and also have the functions of improving immunity, resisting free radicals and preventing aging.

The sodium hyaluronate has the effects of resisting inflammation, inhibiting bacteria, keeping skin smooth, and promoting cell growth, differentiation and repair. The sodium hyaluronate can also improve the physiological condition of skin, provide excellent external environment for the synthesis of dermis collagen and elastic fiber, strengthen the supply of nutrient substances and play a role in protecting skin and caring skin. Sodium hyaluronate can also prevent the production of some enzymes in cells, reduce the formation of free radicals, and play an important role in preventing the free radicals from damaging cell structures, generating lipid peroxidation, causing aging of organisms and the like.

Boric acid has certain astringing effect, and can inhibit bacteria and prevent infection, and can be used for preparing ointment, mainly applied to exudative reaction of skin, and also can be used for treating mild infection such as acute eczema, dermatitis, impetigo, eczema, etc. When the surface has erosion and exudation, 3% boric acid solution is used for wet dressing, and when the liquid is evaporated, some heat can be taken away, so that the dilated blood vessel is contracted, and exudation is reduced. Boric acid can also be used for regulating the pH value of the system, has mild effect and smooth hand feeling, and has no stimulation to skin. Boric acid is weakly acidic, and can be dissolved in water, alcohol, glycerol and other liquids to play a role in corrosion prevention.

Trehalose has excellent properties of maintaining cell viability and biological macromolecule activity. Human epidermal cells are easy to lose moisture and keratinize under the environments of high temperature, high cold, dryness, strong ultraviolet radiation and the like, so that the skin is damaged. Under the condition, the trehalose can form a special protective film on the cell surface layer, so that skin sunburn and melanin precipitation are effectively avoided, and skin aging is resisted. The mucus separated from the membrane not only moistens skin cells, but also has the function of radiating out external heat, thereby protecting the skin from damage.

Berberine is aliased by berberine, and has the main effects of clearing heat, purging pathogenic fire and eliminating dampness. The berberine has broad antibacterial spectrum, and has antibacterial effect on various gram positive and negative bacteria in vitro, wherein the berberine has strong inhibition effect on hemolytic streptococcus, staphylococcus aureus, vibrio cholerae, typhoid bacillus, diphtheria bacillus and the like, and can inhibit bacteria at low concentration and bacteria at high concentration, thereby effectively preventing chilblain. It can also improve local blood circulation, detumescence, heal wound, repair, and has remarkable effect in preventing and treating skin chilblain and rhagadia. In addition, the berberine has good treatment effect on skin sores, ulcers, burns and the like when being externally used.

And the total polysaccharide has the effect of relieving RAW264.7 macrophage inflammation induced by LPS, can reduce the NO and MDA levels induced by lipopolysaccharide, and raise the SOD, GSH and CAT levels, and has the in vitro anti-inflammatory activity; has inhibiting effect on inflammation of carrageenan induced inflammation rat model and complete Freund adjuvant inflammatory rat model, can reduce IL-6, IL-1β and TNF- α levels, and has remarkable anti-inflammatory activity in vivo.

The reed-bazaar gum has a unique cage structure and strong water locking capacity, so that bound water is difficult to release, is an excellent humectant, has excellent safety and can be used as a hand feeling modifier. The water-soluble moisturizing gel has good water solubility, is a transparent moisturizing matrix, can be used for improving skin feel and lubricity of products, has double moisturizing effects, and is easy to be absorbed by skin during massage. In addition, the rufimbriae gum can also improve microcirculation, promote cellular metabolism, regulate pH value of skin, effectively regulate oil secretion and shrink pores.

The hydrogenated lecithin S-10 is a partial hydrolysate of lecithin, can be absorbed by human skin and hair, can promote permeation of other nutrient substances, has strong hydrophilicity and moisture retention, can play roles in moisture retention, emulsification, dispersion, antioxidation and the like in a formula of cosmetics, can be used as a surfactant, and can also condition the skin to achieve a better oil-water balance effect and better absorb nutrition. The hydrogenated lecithin S-10 has strong emulsification effect, can stabilize emulsion, and is suitable for preparing gel products.

The simple multifunctional emulsifier EMT-10 is a solid anionic acrylic polymer, a novel two-in-one powdery polymer, is fast in dissolution in water, is used for thickening aqueous phase solution and promoting the stability of O/W type emulsion, can be used as a thickener, has emulsifying capacity, has good adaptability to lower or higher pH conditions, has stable thickening capacity in a wide pH value range, and has a certain electrolyte tolerance capacity. The EMT-10 emulsifier is convenient to use, has good stability, can be operated at room temperature, has smooth hand feeling and is not sticky.

The Sibirac 305 has excellent performance, extremely strong gel forming capability, excellent emulsifying capability, excellent stabilizing effect and extremely simple application method. The Sibirac 305 is a special emulsifier with quite excellent emulsifying, dispersing and stabilizing properties, can be easily emulsified under simple stirring, forms a very stable emulsifying system, can be independently applied to the production and application of various emulsifying systems, and can also be compounded with other auxiliary emulsifiers for use, thereby playing a role in stabilizing together. The Sibirac 305 has excellent adsorption capacity, can adsorb various nutrients and medicines, and can enable the nutrients and medicines to be wetted for a long time and keep activity; the nutritional agent and the medicine are slowly released on the surface of the skin through the replacement effect, so that various functional components are effectively promoted to permeate into skin tissues, the excessive aggregation of the nutritional agent or the medicine is prevented, the acting force is weakened, the waste is caused, and the more effective skin care and prevention effects are achieved. The Saike 305 has excellent moisturizing effect, can make the product extremely stable and hard to harden, and can obtain a long-term moisturizing effect after being applied to the skin.

The hydrogenated lecithin S-10, the simple multifunctional emulsifier EMT-10 and the Saike 305 have synergistic effect, and the three are combined together, so that the prepared frozen healing paste is finer and more uniform and more stable.

Sodium alginate is a natural polysaccharide with good stability, viscosity and safety, and is mainly used as a thickening agent in cosmetics and skin care products, and has no sticky feel and stiffness after thickening. The sodium alginate has good protection effect on skin, can enhance the water retention and elasticity of the skin, and is suitable for dry skin. In addition, the sodium alginate can remove wrinkles, improve the secretion state of dry skin, shrink pores, make the skin finer and more beautiful, and has certain antibacterial capability.

The ice crystal former AVC is a thickener which has high shear force, is resistant to UV rays, and is stable. The stabilizer can be used as a stabilizer of oil-in-water emulsion, is convenient to use, can be directly dissolved, does not need to be neutralized, is simple to operate, and has better stability even under the condition of no emulsifier. In addition, the product using the ice crystal forming agent AVC has the characteristics of light and thin appearance, low sticky feeling, rheological property, good skin feel and the like.

The amino acid thickener DOE-120T is a high-efficiency thickener extracted from plants, can improve the appearance and the spreadability of paste, is mild and safe, can be matched with various anionic surfactants and common amphoteric surfactants, and reduces the irritation of other components. The eye irritation test result is zero, which proves that the safety is higher, and the addition of the DOE-120T thickener in the formula can obviously reduce the irritation of the strong-irritation surfactant to the skin besides thickening.

The U30 cellulose thickener belongs to natural galactomannan series, is ideal smoothing agent and thickener in cosmetics, can be compounded with various surfactants, and has various functions of antistatic, conditioning, thickening, colloid stabilization, moisture retention, antistatic, wetting, smoothness and the like. The U30 cellulose thickener is used in the product, and can reduce irritation of surfactant, restore skin barrier function, moisten and smooth skin, and increase storage stability of the product.

Carbomer 941 has certain affinity to skin, and has effects of protecting skin, and reducing irritation and injury of irritant substances to skin and mucous membrane. Carbomer 941 has certain looseness, is a slightly acidic substance with extremely strong dilutability, and can reduce the viscosity by adding proper amount of carbomer 941 when preparing the jelly healing paste so as to maintain the stability of the effective substance and endow the matrix with crystal transparent texture.

The triethanolamine has the effects of moisturizing and supplementing water to skin, belongs to the class of partial alkali, and has softer acidity and better skin care effect. Therefore, the triethanolamine is added into the skin care product to adjust the pH of the skin care product, and the skin care product can generally have the functions of moisturizing, thickening and the like. The pH value of the system is regulated by adopting 30% triethanolamine solution, so that the proper consistency and stability of the product are maintained.

The 1, 2-propylene glycol is a small molecular moisturizing component, can lock moisture, plays a role in moisturizing, and has a good role in moisturizing skin as a solvent and a softener. The 1, 2-propylene glycol can also be used as a cold resistant agent for skin, so that the crystallization point is reduced, and the damage of the skin caused by freezing is reduced. Secondly, the 1, 2-propylene glycol has the effect of promoting skin absorption, can be used as a penetrating agent, and can be added into a product to exert moisture retention and enable the components of the cream to be absorbed by the skin better. In addition, it can stabilize the formulation, is safe and non-irritating, and can be used for preparing various ointments, solvents for ointments, softeners, etc. In the formula, the cream can also be used as a thickening agent, a stabilizing agent and a solubilizing agent, so that the cream is more moist and is easy to apply.

Zedoary turmeric oil has effects of promoting qi and blood circulation, and sterilizing in a synergistic way; sterilizing and diminishing inflammation of calendula; the aloe oil can effectively resist bacteria and diminish inflammation, inhibit the reproduction of pathogenic microorganisms and bacteria on the surface of the skin, and prevent the reproduction of microorganisms on the body surface; emu oil has antiinflammatory and analgesic effects; cherry has sweat promoting, eruption promoting, and toxic materials clearing away effects; the herba Sedi Aizoon extract has in vitro antibacterial effect, and can inhibit Staphylococcus aureus, surface staphylococci and Micrococcus, and the ursolic acid contained in herba Sedi Aizoon has various biological effects such as tranquilizing, antiinflammatory and antibacterial effects; the spina gleditsiae can inhibit or kill various gram positive bacteria and negative bacteria, and has an inhibiting effect on staphylococcus aureus; the Paederia scandens has better effects of clearing heat and detoxicating, resisting bacteria and diminishing inflammation, and can inhibit bacterial growth and remove toxins; boric acid has certain antibacterial, astringent and anti-infection effects; berberine has antibacterial effect on various gram positive and negative bacteria; the total polysaccharide has in vivo and in vitro anti-inflammatory activity; sodium alginate has certain antibacterial ability. In a word, each component supplements each other, and plays a role in clearing away heat and toxic materials, resisting bacteria and diminishing inflammation.

Calendula can effectively relieve eczema and skin itching; the aloe vera can relieve itching and pain; essential fatty acids contained in evening primrose oil can relieve symptoms of pain and itching caused by dry skin and even inflammation; the cherry squeezed juice is smeared on the wound part, and can immediately astringe and relieve pain; watermelon Pi Fu contains multiple vitamins, and can be used for relieving skin itching. In a word, each component supplements each other, and plays a role in astringing and relieving itching, pain and relief.

The rose hip oil has the effects of moisturizing skin, and promoting skin to be tender, soft and whitening; the calendula is rich in multiple vitamins, so that the gloss and elasticity of the skin can be improved, and the skin can be gently moistened; the aloe oil contains natural gel substances, retains water and lignin, has strong permeation substances, and can help nutrients permeate into skin; the main active ingredients of the evening primrose oil are mostly unsaturated fatty acid, so that the evening primrose oil can prevent pores from being blocked and nourish skin; the shea butter contains a large amount of nutrients, and can promote the regeneration of epidermal cells and give nutrition to the skin. In addition, the shea butter contains triglyceride, can form a protective layer on the surface of skin, prevents water evaporation and plays a role in moisturizing; the strawberry seed oil contains rich moisturizing nutrient components, can increase the release amount of hyaluronic acid of cells, and can greatly enhance the moisturizing effect; emu oil can moisten skin sufficiently, promote skin luster, smooth and tender, and has moisturizing effect on skin; the sodium alginate can enhance the water retention and elasticity of the skin and has better protective effect on the skin. In a word, each component supplements each other, and plays a role in preventing and moisturizing, and preserving moisture and skin.

Zedoary turmeric oil promotes healing of pathological tissues; the rose hip oil promotes the activity of epidermal cells to improve the skin regeneration and regeneration capacity, has excellent effects on repairing and healing damaged skin caused by chilblain, and can also repair skin and lighten scar; the calendula can accelerate the healing of the sore, and effectively relieve and repair the skin which is easy to be red and swollen and sensitive; the aloe oil can balance and inhibit sebum secretion and promote wound healing; evening primrose oil can help wound healing and lighten whelks and black spots; the strawberry seeds can also effectively promote the proliferation of keratinocytes, improve skin inflammation and regulate skin barrier function; the deep penetrating ability of emu oil promotes healing of burns, eczema, dermatitis and other skin disorders; the cherry is smeared on the wound part, can play roles of astringing and relieving pain, preventing foaming and suppuration at the wound part and desalting scars; the arbutin contained in the aizoon stonecrop herb can reduce the generation of melanin by inhibiting the activity of enzyme tyrosinase for producing melanin, and accelerate the decomposition and excretion of the melanin by combining the aizoon stonecrop herb with tyrosinase, thereby reducing skin pigmentation and removing scars; spina Gleditsiae can promote skin cell growth; watermelon peel can promote wound healing, reduce scar, and reduce skin melanin pigmentation; sodium hyaluronate directly promotes cell growth, differentiation, reconstitution and repair; berberine can also improve local blood circulation, detumescence, heal wound, repair, and has remarkable effect in preventing and treating skin chilblain and rhagadia. In a word, each component supplements each other, and plays a role in healing sore and removing scar together.

The jelly-curing cream prepared by the invention is bright yellow, has proper viscosity, is fine, smooth and uniform, is light and moist, is bright and white, has good spreadability, quick absorption and fragrant smell.

Compared with the prior art, the prepared jelly-healing cream can inhibit bacteria, relieve itching, prevent and repair cracks, deeply moisten skin, regulate the moisture of the skin, strengthen the natural moisture-preserving layer of the skin, continuously supplement the moisture and the nutrients required by the skin, thoroughly change the skin state, enable the skin to be full, water and elastic, and strengthen the resistance of the skin to cold environment. The jelly-curing cream provided by the invention has the functions of inhibiting bacteria, relieving itching, resisting inflammation, diminishing swelling and repairing, can effectively treat chilblain and prevent the chilblain from recrudescence, meets the needs of the masses, and has a wide market prospect.

Drawings

FIG. 1 is a picture of an anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-healing paste prepared by the invention;

FIG. 2 is a cold test of the jelly-curing cream prepared in example 2; a is before cold experiment; b is after cold experiment;

FIG. 3 is a thermal test of the jelly-healing cream prepared in example 2; a is before a thermal experiment; b is after thermal experiment;

FIG. 4 is a centrifugation experiment of the lyophilized extract prepared in example 2; a is before a centrifugal experiment; b is after the centrifugal experiment;

FIG. 5 is a room temperature standing experiment of the jelly-like ointment prepared in example 2; a is before being placed at room temperature; after the graph B is placed at room temperature;

FIG. 6 is an experiment of irritation and allergy of the prepared paste of example 2 to normal skin rats;

FIG. 7 is an experiment of irritation and allergy of the cream prepared in example 2 to skin-injured rats;

FIG. 8 is a stimulus and allergy test of the prepared paste of example 2 to a test person; a is before administration, B is when administration, C is after administration for 30min;

FIG. 9 is a microscopic image of the paste prepared in example 2; a is 200×; b is 400X;

FIG. 10 is a regression line graph of the permeation experiments of the flupirocin ointment prepared in example 2;

FIG. 11 is a permeation test of the jelly-roll prepared in example 2; in the figure, mupirocin ointment is arranged in a test tube 1, and frozen healing ointment is arranged in a test tube 2;

FIG. 12 is a graph showing the condition of hind foot plantar swelling in the right foot of each group of rats;

Fig. 13 is a change in plantar swelling rate (%) ( n=6) of each group of rats;

FIG. 14 shows the effect of various concentrations of cream on NO content;

FIG. 15 is a graph showing the effect of various concentrations of cryohealing paste on the expression level of oxidative stress indicators;

FIG. 16 is a graph showing the effect of different doses on IL-1β, IL-6, IL-10 and TNF- α levels;

FIG. 17 is the effect on PGE2 content in the right hind paw of the rat;

fig. 18 shows the frequency of itch occurrence for each group of rats ( n=6);

fig. 19 is a picture showing failure in preparing the cream.

Detailed Description

The invention is further described below with reference to examples.

According to pharmacopoeia regulations, coarse powder refers to powder which can pass through a second sieve completely but is mixed with powder which can pass through a fourth sieve by no more than 40%; all heat extractions were performed in the micro-boiling state. The ethanol solution concentration referred to in the present invention is a volume concentration.

Example 1

A preparation method of an anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing paste comprises the following steps:

1.1 preparation of calendula soaking oil

Weighing appropriate amount of calendula flower petals, adding olive oil with 13 times of calendula flower petal mass, extracting at ultrasonic frequency 20000Hz for 0.6 hr, soaking at room temperature for 8 days, and filtering with four layers of gauze to obtain calendula soaking oil.

1.2 Preparation of cherry extract

Removing the pit of fresh cherry, weighing a proper amount of pulp, putting into a juicer, adding distilled water 3 times the mass of cherry pulp, squeezing juice for 1.5min in a juice squeezing mode, filtering with four layers of gauze to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 2.5 mL/g times the mass of cherry pulp, thus obtaining cherry squeezing liquid for later use.

1.3 Preparation of watermelon peel extract

Weighing a proper amount of chopped fresh watermelon peel, putting into a juicer, adding distilled water 3 times the mass of the watermelon peel, squeezing juice for 1.5min in a juice squeezing mode, filtering to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 2 mL/g times the mass of the watermelon peel, thus obtaining watermelon peel squeezing liquid for later use.

1.4 Preparation of Sedum aizoon extract

Weighing appropriate amount of coarse powder of radix Notoginseng, and extracting under reflux for 3 times: adding 16 times of water into the coarse powder of the aizoon stonecrop herb for soaking for 0.25h and extracting for 0.5h; adding water 10 times of the coarse powder of the aizoon stonecrop herb for 2 nd time, and extracting for 0.5h; adding water with the mass of 8 times of that of the coarse powder of the aizoon stonecrop herb for 3 times, and extracting for 0.5h; filtering with four layers of gauze each time, mixing the filtrates, vacuum filtering, and concentrating the filtrate volume to 3 times of the coarse powder mass of herba Sedi to obtain herba Sedi extractive solution.

1.5 Preparation of spina Gleditsiae extract

Weighing appropriate amount of spina Gleditsiae coarse powder, and extracting under reflux for 3 times: adding 70% ethanol solution 15 times of the weight of spina Gleditsiae coarse powder, soaking for 0.30 hr, and extracting for 1.5 hr; adding 70% ethanol solution 9 times of the weight of the spina gleditsiae coarse powder for 2 times, and extracting for 1.0h; adding 70% ethanol solution with the mass of 8 times of that of the spina gleditsiae coarse powder for 3 times, and extracting for 0.6h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of the mass of spina Gleditsiae coarse powder to obtain spina Gleditsiae extractive solution.

1.6 Preparation of the white extract of Paederia scandens

Weighing appropriate amount of Paederia scandens, and extracting under reflux for 3 times: adding 15 times of water for the white matter of the chicken for 1 st time, soaking for 5min, and extracting for 1.5h; adding 11 times of water for the white matter of the chicken for the 2 nd time, and extracting for 1.0h; adding 9 times of water for the amount of the white matter of the chicken for 3 times, and extracting for 0.5h; filtering with four layers of gauze each time, mixing filtrates, filtering, and concentrating the filtrate volume to 3 times of the quality of the Paederia scandens extract.

1.7 Preparation of Myrica rubra extract

Weighing a proper amount of waxberry, and extracting for 3 times by heating and reflux: adding 15 times of water to the waxberry for 1 st time, and extracting for 1.5h; adding 10 times of water to the waxberry for 1.0h; adding water 8 times of the weight of the waxberry for the 3 rd time, and extracting for 0.5h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of mL/g of fructus Myricae Rubrae mass to obtain fructus Myricae Rubrae extractive solution.

1.8 Preparation of Total polysaccharide from Hemsleya cordata

Weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding 15 times of water to the coarse powder of the radix stephaniae tetrandrae, soaking for 20min, and extracting for 1.0h; adding water 10 times of the mass of the coarse powder of the radix stephaniae tetrandrae for 2 times, and extracting for 1.0h; adding water with the mass of 8 times of that of the coarse powder of the radix stephaniae tetrandrae for 3 times, and extracting for 0.5h; filtering with four layers of gauze each time, mixing filtrates, centrifuging at 4deg.C and 8000r/min for 10min, and concentrating the clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder. Precipitating with 95% ethanol solution at volume ratio of 1:7, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, oven drying the filter cake, pulverizing, and sieving with 80 mesh sieve to obtain total polysaccharide.

1.9 Preparation of anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing ointment

A) Weighing 1.1g of amino acid thickener DOE-120T, adding into 12.0mL of distilled water, stirring, mixing, placing into a water bath kettle with a temperature of 63 ℃ heated in advance, continuing stirring for 6min, taking out, standing for 12h at normal temperature, and fully swelling to obtain amino acid thickener DOE-120T gel solution for later use;

b) Weighing 0.32g of U30 cellulose thickener, adding into 30.0mL of distilled water, stirring, mixing well, standing at normal temperature for 12h, and fully swelling to obtain U30 cellulose thickener gel solution for later use;

c) Weighing 0.33g of sodium alginate, adding into 27.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 63 ℃ heated in advance, continuing stirring for 5min, taking out, standing for 12h at normal temperature, and obtaining uniform and transparent sodium alginate gel liquid after the sodium alginate is fully swelled for later use;

d) Weighing 0.07g of ice crystal forming agent AVC, adding into 12.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 63 ℃ heated in advance, continuing stirring for 7min, taking out, standing for 12h at normal temperature, and obtaining uniform and transparent ice crystal forming agent AVC gel solution for standby;

e) Weighing 0.55g of carbomer 941, adding into 42.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 63 ℃ heated in advance, continuing stirring for 7min, taking out, standing for 12h at normal temperature, and obtaining uniform and transparent carbomer 941 gel solution for later use;

f) Weighing 0.32g of zedoary turmeric oil, 0.63g of rosehip oil, 0.42g of calendula soaking oil, 0.53g of aloe oil, 0.21g of evening primrose oil, 0.8g of shea butter, 0.4g of strawberry seed oil, 0.5g of emu oil and 0.12g of hydrogenated lecithin S-10, placing into a beaker, mixing and stirring, heating and melting in a water bath pot with a temperature of 75 ℃ which is heated in advance, and uniformly stirring after melting to serve as an oil phase for standby;

g) Weighing 0.12g of simple multifunctional emulsifier EMT-10 and 0.11g of Saike 305, adding 11.0mL of distilled water, stirring to uniformly mix, heating in a water bath kettle with the temperature of 75 ℃ heated in advance, and uniformly stirring to serve as a water phase for later use;

i) 0.2mL of cherry extract, 0.55mL of aizoon stonecrop herb extract, 0.11mL of spina gleditsiae extract, 0.3mL of Paederia scandens extract, 0.1mL of watermelon peel extract, 0.5mL of waxberry extract, 0.1g of boric acid, 0.11g of luba glue, 0.001g of berberine coptis chinensis, 0.1g of trehalose and 0.05g of total polysaccharide are taken, stirred and dissolved, and uniformly mixed to obtain a solution A for standby;

j) Weighing 0.36g of ethylparaben, adding 1.0mL of 1, 2-propanediol, stirring and dissolving to obtain preservative solution for later use;

k) Adding the prepared solution A into emulsion matrix, stirring and mixing uniformly, adding 1.0mL of 1% sodium hyaluronate solution, 1-120T amino acid thickener gel solution, U30 cellulose thickener gel solution, sodium alginate gel solution, ice crystal forming agent AVC gel solution, carbomer 941 gel solution, 0.5mL of 30% triethanolamine solution, preservative solution and 0.05mL of cucumber essence, stirring and mixing uniformly, and obtaining the antibacterial, antipruritic, anti-inflammatory, detumescence, anti-cracking and repairing jelly.

Example 2

2.1 preparation of calendula soaking oil

Weighing appropriate amount of calendula flower petals, adding olive oil with mass 18 times that of calendula flower petals, extracting at ultrasonic frequency 35000Hz for 0.7h, soaking at room temperature for 10 days, and filtering with four layers of gauze to obtain calendula soaking oil for use.

2.2 Preparation of cherry extract

Removing the pit of fresh cherry, weighing a proper amount of pulp, putting into a juicer, adding 4 times of distilled water of the cherry pulp, squeezing juice for 1.6min in a juice squeezing mode, filtering with four layers of gauze to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 2.5 times of mL/g of the cherry pulp, thus obtaining cherry squeezing liquid for later use.

2.3 Preparation of watermelon peel extract

Weighing a proper amount of chopped fresh watermelon peel, putting into a juicer, adding distilled water with the mass of 4 times of the watermelon peel, squeezing juice for 1.7min in a juice squeezing mode, filtering to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 2 times of the mass of the watermelon peel, namely obtaining watermelon peel squeezing liquid for later use.

2.4 Preparation of Sedum aizoon extract

Weighing appropriate amount of coarse powder of radix Notoginseng, and extracting under reflux for 3 times: adding 17 times of water into the coarse powder of the aizoon stonecrop herb for soaking for 0.3h and extracting for 1h; adding 11 times of water to the coarse powder of the aizoon stonecrop herb for 0.7h; adding water with the mass of 8 times of that of the coarse powder of the aizoon stonecrop herb for 3 times, and extracting for 0.7h; filtering with four layers of gauze each time, mixing the filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of the coarse powder mass of herba Sedi to obtain herba Sedi extractive solution.

2.5 Preparation of spina Gleditsiae extract

Weighing appropriate amount of spina Gleditsiae coarse powder, and extracting under reflux for 3 times: adding 17 times of 70% ethanol solution, soaking for 0.50 hr, and extracting for 1.7 hr; adding 70% ethanol solution 9 times of the weight of the spina gleditsiae coarse powder for 2 times, and extracting for 1.2h; adding 70% ethanol solution with the mass of 8 times of that of the spina gleditsiae coarse powder for 3 times, and extracting for 0.7h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 5 times of the mass of spina Gleditsiae coarse powder to obtain spina Gleditsiae extractive solution.

2.6 Preparation of the white extract of Paederia scandens

Weighing appropriate amount of Paederia scandens, and extracting under reflux for 3 times: adding 17 times of water for the white matter of the chicken for 1 st time, soaking for 10min, and extracting for 1.7h; adding 10 times of water for the white matter of the chicken for the 2 nd time, and extracting for 1.2h; adding water with the mass of 8 times of that of the white chicken in the 3 rd time, and extracting for 0.7h; filtering with four layers of gauze each time, mixing filtrates, filtering, and concentrating the filtrate volume to 5 times of the quality of the Paederia scandens extract.

2.7 Preparation of Myrica rubra extract

Weighing a proper amount of waxberry, and extracting for 3 times by heating and reflux: adding water 16 times of the weight of the waxberry for 1 st time, and extracting for 1.7h; adding 12 times of water to the waxberry for 1.2 hours; adding 9 times of water to the waxberry for 0.7h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of mL/g of fructus Myricae Rubrae mass to obtain fructus Myricae Rubrae extractive solution.

2.8 And the total hexose polysaccharide is prepared by the following method:

Weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding water 16 times the mass of the coarse powder of the radix stephaniae tetrandrae for soaking for 25min, and extracting for 1.2h; adding 12 times of water to the coarse powder of the root of Chinese angelica and extracting for 1.0h; adding water with the mass of 8 times of that of the coarse powder of the radix stephaniae tetrandrae for 3 times, and extracting for 0.8h; filtering with four layers of gauze each time, mixing filtrates, centrifuging at 4deg.C and 8000r/min for 20min, and concentrating the clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder. And precipitating with 95% ethanol at volume ratio of 1:4, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, oven drying the filter cake, pulverizing, and sieving with 80 mesh sieve to obtain total polysaccharide.

2.9 Preparation of anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing ointment

A) Weighing 1.5g of amino acid thickener DOE-120T, adding into 20.0mL of distilled water, stirring, mixing, placing into a water bath kettle with a temperature of 75 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 15h at normal temperature, and fully swelling to obtain amino acid thickener DOE-120T gel solution for later use;

b) Weighing 0.4g of U30 cellulose thickener, adding into 40.0mL of distilled water, stirring, mixing uniformly, standing for 12h at normal temperature, and fully swelling to obtain U30 cellulose thickener gel solution for later use;

c) Weighing 0.7g of sodium alginate, adding into 30.0mL of distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 75 ℃ heated in advance, continuing stirring for 15min, taking out, standing for 17h at normal temperature, and obtaining uniform and transparent sodium alginate gel liquid after the sodium alginate gel liquid is fully swelled for later use;

d) Weighing 0.10g of ice crystal forming agent AVC, adding into 25.0mL of distilled water, stirring, mixing uniformly, placing into a water bath kettle with a temperature of 75 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 17h at normal temperature, and obtaining uniform transparent ice crystal forming agent AVC gel solution for standby;

e) Weighing 0.7g of carbomer 941, adding into 40.0mL of distilled water, stirring uniformly, placing into a water bath kettle with a temperature of 75 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 12h at normal temperature, and obtaining uniform and transparent carbomer 941 gel solution after the carbomer 941 fully swells for later use;

f) Weighing 0.6g of zedoary turmeric oil, 0.8g of rosehip oil, 0.7g of calendula soaking oil, 1.0g of aloe oil, 0.7g of evening primrose oil, 1.0g of shea butter, 0.8g of strawberry seed oil, 0.8g of emu oil and 1.0g of hydrogenated lecithin S-10, placing into a beaker, mixing and stirring, heating and melting in a water bath pot with the temperature of 75 ℃ heated in advance, uniformly stirring after melting, and taking the mixture as an oil phase for standby;

g) Weighing 0.5g of simple multifunctional emulsifier EMT-10 and 0.7g of Saike 305, adding 12.0mL of distilled water, stirring to uniformly mix, heating in a water bath kettle with the temperature of 75 ℃ heated in advance, and uniformly stirring to serve as a water phase for later use;

i) 0.5mL of cherry extract, 0.7mL of aizoon stonecrop extract, 0.5mL of spina gleditsiae extract, 0.4mL of Paederia scandens extract, 0.4mL of watermelon peel extract, 0.7mL of waxberry extract, 0.15g of boric acid, 0.5g of ruba glue, 0.002g of berberine, 0.15g of trehalose and 0.10g of total polysaccharide are taken, stirred and dissolved, and uniformly mixed to obtain a solution A for standby;

j) Weighing 0.46g of ethylparaben, adding 2.0mL of 1, 2-propanediol, stirring and dissolving to obtain preservative solution for later use;

k) Adding the prepared solution A into emulsion matrix, stirring and mixing uniformly, adding 2.0mL of 1% sodium hyaluronate solution, amino acid thickener DOE-120T gel solution, U30 cellulose thickener gel solution, sodium alginate gel solution, ice crystal forming agent AVC gel solution, carbomer 941 gel solution, 0.7mL of 30% triethanolamine solution, preservative solution and 0.15mL of osmanthus fragrans essence, stirring and mixing uniformly, and obtaining the anti-bacterial, antipruritic, anti-inflammatory, detumescence, anti-cracking and repairing jelly.

Example 3

3.1 preparation of calendula soaking oil

Weighing appropriate amount of calendula flower petals, adding olive oil with 20 times of calendula flower petal mass, extracting at ultrasonic frequency 40000Hz for 1.5h, soaking at room temperature for 14 days, and filtering with four layers of gauze to obtain calendula soaking oil for use.

3.2 Preparation of cherry extract

Removing the pit of fresh cherry, weighing a proper amount of pulp, putting into a juicer, adding distilled water 5 times the mass of cherry pulp, squeezing juice for 2.0min in a juice squeezing mode, filtering with four layers of gauze to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 3 mL/g times the mass of cherry pulp, thus obtaining cherry squeezing liquid for later use.

3.3 Preparation of watermelon peel extract

Weighing a proper amount of chopped fresh watermelon peel, putting into a juicer, adding distilled water which is 5 times of the watermelon peel in mass, squeezing juice for 2.0min in a juice squeezing mode, filtering to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 3 times of the watermelon peel mass mL/g, thus obtaining watermelon peel squeezing liquid for later use.

3.4 Preparation of Sedum aizoon extract

Weighing appropriate amount of coarse powder of radix Notoginseng, and extracting under reflux for 3 times: adding 18 times of water into the coarse powder of the aizoon stonecrop herb for soaking for 0.75h and extracting for 1h; adding 14 times of water into the coarse powder of the aizoon stonecrop herb for extraction for 1h; adding water 12 times of the coarse powder of the aizoon stonecrop herb for extraction for 1h; filtering with four layers of gauze each time, mixing the filtrates, vacuum filtering, and concentrating the filtrate volume to 7 times of the coarse powder mass of herba Sedi to obtain herba Sedi extractive solution.

3.5 Preparation of spina Gleditsiae extract

Weighing appropriate amount of spina Gleditsiae coarse powder, and extracting under reflux for 3 times: adding 70% ethanol solution with the mass of 18 times of that of the spina gleditsiae coarse powder for 1 st time, soaking for 0.75h, and extracting for 2.0h; adding 70% ethanol solution 10 times of the weight of the spina Gleditsiae coarse powder for 2 times, and extracting for 1.5h; adding 70% ethanol solution with the mass of 8 times of that of the spina gleditsiae coarse powder for 3 times, and extracting for 1.0h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 8 times of the mass of spina Gleditsiae coarse powder to obtain spina Gleditsiae extractive solution.

3.6 Preparation of the white extract of Paederia scandens

Weighing appropriate amount of Paederia scandens, and extracting under reflux for 3 times: adding water with the amount of the white matter of the chicken being 18 times for soaking for 15min, and extracting for 2.0h; adding 12 times of water for the white matter of the chicken for the 2 nd time, and extracting for 1.5h; adding water with the mass of 8 times of that of the white chicken in the 3 rd time, and extracting for 1.0h; filtering with four layers of gauze each time, mixing filtrates, filtering, and concentrating the filtrate volume to 5 times of the quality of the Paederia scandens extract.

3.7 Preparation of Myrica rubra extract

Weighing a proper amount of waxberry, and extracting for 3 times by heating and reflux: adding 18 times of water to the waxberry for 2.0h; adding 15 times of water to the waxberry for the 2 nd time, and extracting for 1.5h; adding 10 times of water to the waxberry for 1.0h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of mL/g of fructus Myricae Rubrae mass to obtain fructus Myricae Rubrae extractive solution.

3.8 Preparation of Total polysaccharide from Hemsleya cordata

Weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding distilled water with the mass 18 times of that of the coarse powder of the radix stephaniae tetrandrae for 1 st time, soaking for 45min, and extracting for 1.6h; adding 13 times of water to the coarse powder of the root of the Chinese angelica and extracting for 1.6 hours; adding water 12 times of the mass of the radix stephaniae tetrandrae coarse powder for 3 times, and extracting for 1.2h; filtering with four layers of gauze each time, mixing filtrates, centrifuging at 4deg.C for 25min at 8000r/min, and concentrating the clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder. And precipitating with 95% ethanol at volume ratio of 1:2, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, oven drying the filter cake, pulverizing, and sieving with 80 mesh sieve to obtain total polysaccharide.

3.9 Anti-inflammatory, detumescence, antibacterial, antipruritic, anticracking and repairing jelly-curing ointment

A) Weighing 3.0g of amino acid thickener DOE-120T, adding into 50.0mL of distilled water, stirring, mixing, placing into a water bath kettle with the temperature of 80 ℃ heated in advance, continuing stirring for 20min, taking out, standing for 24h at normal temperature, and fully swelling to obtain amino acid thickener DOE-120T gel solution for later use;

b) Weighing 0.7g of U30 cellulose thickener, adding into 65.0mL of distilled water, stirring, mixing uniformly, standing for 24 hours at normal temperature, and obtaining a U30 cellulose thickener gel solution after the U30 cellulose thickener is fully swelled for later use;

c) Weighing 1.5g of sodium alginate, adding into 49mL of distilled water, stirring uniformly, placing into a water bath kettle with 80 ℃ heated in advance, continuing stirring for 19min, taking out, standing for 24h at normal temperature, and obtaining uniform and transparent sodium alginate gel solution after the sodium alginate is fully swelled for later use;

d) Weighing 0.20g of ice crystal forming agent AVC, adding into 50.0mL of distilled water, stirring, mixing uniformly, placing into a water bath kettle with the temperature of 80 ℃ heated in advance, continuing stirring for 20min, taking out, standing for 24h at normal temperature, and obtaining uniform transparent ice crystal forming agent AVC gel solution for standby;

e) Weighing carbomer 941.0 g, adding into 69.0mL distilled water, stirring uniformly, placing into a water bath kettle with the temperature of 80 ℃ heated in advance, continuing stirring for 20min, taking out, standing for 24h at normal temperature, and obtaining uniform and transparent carbomer 941 gel solution after full swelling;

f) 1.1g of zedoary turmeric oil, 1.1g of rosehip oil, 1.3g of calendula soaking oil, 1.4g of aloe oil, 1.0g of evening primrose oil, 1.6g of shea butter, 1.3g of strawberry seed oil, 1.5g of emu oil and 2.0g of hydrogenated lecithin S-10 are weighed, placed in a beaker, mixed and stirred, and placed in a water bath kettle heated in advance for heating and melting at 80 ℃ to be uniformly stirred as an oil phase for standby;

g) Weighing 1.1g of simple multifunctional emulsifier EMT-10 and 1.1g of Saike 305, adding 14.0mL of distilled water, stirring to uniformly mix, heating in a water bath kettle with the temperature of 80 ℃ heated in advance, and uniformly stirring to serve as a water phase for later use;

i) 1.0mL of cherry extract, 1.1mL of aizoon stonecrop extract, 1.2mL of spina gleditsiae extract, 1.0mL of Paederia scandens extract, 1.5mL of watermelon peel extract, 1.3mL of waxberry extract, 0.2g of boric acid, 1.0g of luba glue, 0.005g of berberine coptis chinensis, 0.25g of trehalose and 0.15g of total polysaccharide are taken, stirred and dissolved, and uniformly mixed to obtain a solution A for standby;

j) Weighing 0.83g of ethylparaben, adding 4.0mL of 1, 2-propanediol, stirring and dissolving to obtain preservative solution for later use;

k) Adding the prepared solution A into emulsion matrix, stirring and mixing uniformly, adding 2.0mL of 1% sodium hyaluronate solution, amino acid thickener DOE-120T gel solution, U30 cellulose thickener gel solution, sodium alginate gel solution, ice crystal forming agent AVC gel solution, carbomer 941 gel solution, 1.0mL of 30% triethanolamine solution, preservative solution and 0.30mL of lavender essence, stirring and mixing uniformly, and obtaining the anti-bacterial, antipruritic, anti-inflammatory, detumescence, anti-cracking and repairing jelly.

Example 4 (comparative example)

4.1 preparation of calendula oil

Weighing appropriate amount of calendula flower petals, adding olive oil with 15 times of calendula flower petal mass, extracting at ultrasonic frequency of 20000Hz for 1.0h, soaking at room temperature for 10 days, and filtering with four layers of gauze to obtain calendula oil for use.

4.2 Preparation of cherry extract

Weighing a proper amount of fresh cherry, putting the fresh cherry into a juicer, adding distilled water 3 times the cherry mass, squeezing juice for 2.0min in a juicing mode, filtering with four layers of gauze to obtain filtrate, and carrying out suction filtration, wherein the volume of the filtrate is 3 mL/g of the cherry weight, thus obtaining cherry squeezing liquid for later use.

4.3 Preparation of watermelon peel extract

4.4 Preparation of Sedum aizoon extract

Weighing appropriate amount of coarse powder of radix Notoginseng, and extracting under reflux for 3 times: adding 15 times of water into the coarse powder of the aizoon stonecrop herb for soaking for 0.5h and extracting for 1.0h; adding water 10 times of the coarse powder of the aizoon stonecrop herb for 2 times, and extracting for 1h; adding water with the mass of 8 times of that of the coarse powder of the aizoon stonecrop herb for 3 times, and extracting for 0.5h; filtering with four layers of gauze each time, mixing the filtrates, vacuum filtering, and concentrating the filtrate volume to 5 times of the weight of herba Sedi coarse powder to obtain herba Sedi extractive solution.

4.5 Preparation of spina Gleditsiae extract

Weighing appropriate amount of spina Gleditsiae coarse powder, and extracting under reflux for 3 times: adding 15 times of 70% ethanol solution into the spina gleditsiae coarse powder for 1 st time, soaking for 0.5h, and extracting for 2.0h; adding 70% ethanol solution 10 times of spina Gleditsiae coarse powder for 2 times, and extracting for 1.5 hr; adding 70% ethanol solution 8 times of spina Gleditsiae coarse powder for 3 times, and extracting for 1.0 hr; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 5 times of spina Gleditsiae weight mL/g to obtain spina Gleditsiae extractive solution.

4.6 Preparation of the white extract of Paederia scandens

Weighing appropriate amount of Paederia scandens, and extracting under reflux for 3 times: adding 15 times of water for 1 st time, soaking for 15min, and extracting for 2.0h; adding 13 times of water for 2 nd time, and extracting for 1.5h; adding 9 times of water for 3 times, and extracting for 1.0h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of the weight of the Paederia scandens L/g to obtain Paederia scandens extract for later use.

4.7 Preparation of Myrica rubra extract

Weighing a proper amount of waxberry, and extracting for 3 times by heating and reflux: adding 15 times of water for 1 st time, and extracting for 2.0h; adding 10 times of water for 2 nd time, and extracting for 1.5h; adding 8 times of water for 3 times, and extracting for 1.0h; filtering with four layers of gauze each time, mixing filtrates, vacuum filtering, and concentrating the filtrate volume to 4 times of the weight of fructus Myricae Rubrae to obtain fructus Myricae Rubrae extractive solution.

4.8 Preparation of Total polysaccharide from Hemsleya cordata

Weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding distilled water 15 times of the mass of the coarse powder of the radix stephaniae tetrandrae at the 1 st time, soaking for 20min, and extracting for 1.5h; adding 13 times of water to the coarse powder of the root of the Chinese angelica and extracting for 1.5 hours; adding 12 times of water to the coarse powder of the radix stephaniae tetrandrae, and extracting for 1.0h; filtering with four layers of gauze each time, mixing filtrates, centrifuging at 4deg.C and 8000r/min for 15min, and concentrating the clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder. And precipitating with 95% ethanol solution at volume ratio of 1:4, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, and oven drying the filter cake to obtain total polysaccharide.

4.9 Preparation of anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing paste:

a) Weighing 3.0g of amino acid thickener DOE-120T, adding into 50.0mL of distilled water, stirring, mixing, placing into a water bath kettle with a temperature of 65 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 24h at normal temperature, and fully swelling to obtain amino acid thickener DOE-120T gel solution for later use;

b) Weighing 0.5g of U30 cellulose thickener, adding into 50.0mL of distilled water, stirring, mixing uniformly, standing for 24 hours at normal temperature, and obtaining a U30 cellulose thickener gel solution after the U30 cellulose thickener is fully swelled for later use;

c) Weighing 0.5g of sodium alginate, adding into 30.0mL of distilled water, stirring uniformly, placing into a 65 ℃ water bath kettle heated in advance, continuing stirring for 10min, taking out, standing for 24h at normal temperature, and obtaining uniform and transparent sodium alginate gel solution after the sodium alginate is fully swelled for later use;

d) Weighing 0.1g of ice crystal forming agent AVC, adding into 30.0mL of distilled water, stirring, mixing uniformly, placing into a water bath kettle with a temperature of 65 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 24h at normal temperature, and obtaining uniform transparent ice crystal forming agent AVC gel solution for standby;

e) Weighing 0.5g of carbomer 941, adding into 50.0mL of distilled water, stirring uniformly, placing into a water bath kettle with a temperature of 65 ℃ heated in advance, continuing stirring for 10min, taking out, standing for 24h at normal temperature, and obtaining uniform and transparent carbomer 941 gel solution for later use;

f) Weighing 0.5g of zedoary turmeric oil, 0.6g of rosehip oil, 0.5g of calendula oil, 0.5g of aloe oil, 0.5g of evening primrose oil, 0.8g of shea butter, 0.5g of strawberry seed oil, 0.5g of emu oil and 0.6g of hydrogenated lecithin S-10, placing into a beaker, mixing and stirring, heating in a water bath kettle at 85 ℃ to serve as an oil phase for standby;

g) Weighing 0.2g of simple multifunctional emulsifier EMT-10 and 0.5g of Saike 305, adding 10.0mL of distilled water, stirring to uniformly mix, and heating in a water bath kettle at 85 ℃ to serve as a water phase for later use;

i) 1.0mL of cherry extract, 1.0mL of aizoon stonecrop extract, 1.0mL of spina gleditsiae extract, 1.0mL of Paederia scandens extract, 1.0mL of watermelon peel extract, 1.0mL of waxberry extract, 0.3g of boric acid, 0.5g of luba glue, 0.1g of berberine of coptis root, 0.3g of trehalose and 0.05g of total polysaccharide are taken, stirred and dissolved, and uniformly mixed to obtain a solution A for standby;

j) Weighing 0.45g of ethylparaben, adding 1.0mL of 1, 2-propanediol, stirring and dissolving to obtain preservative solution for later use;

k) Adding the solution A obtained by the preparation into emulsion matrix, stirring and mixing uniformly, adding 3.0mL of 1% sodium hyaluronate solution, amino acid thickener DOE-120T gel solution, U30 cellulose thickener gel solution, sodium alginate gel solution, ice crystal forming agent AVC gel solution, carbomer 941 gel solution, 0.8mL of 30% triethanolamine solution, preservative solution and jasmine essence, stirring and mixing uniformly, thus obtaining the anti-inflammatory, detumescent, antibacterial, antipruritic, anticracking and repairing jelly, as shown in figure 19, wherein the matrix of failure embodiment is uneven, the granular feel is obvious, and the improper proportion of each component is caused.

Embodiment reasons for failure of the jelly healing cream: the berberine dosage is large, so that the prepared paste has granular feel and unacceptable smell.

Example 5 (as a comparative example)

A preparation method of anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly is the same as in example 1, except that step f) is not 0.6g of rose hip oil, 0.5g of calendula oil, but 0.6g of shea butter and 0.5g of sea buckthorn fruit oil, and the prepared jelly has bad taste and is unacceptable and no subsequent experiment is carried out.

Example 6 (as a comparative example)

The preparation method of the anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing cream is similar to the example 1, and is different from the following cases:

1. during preparation, hydrogenated lecithin S-10, simple multifunctional emulsifier EMT-10 and Sibiricarb 305 are all in water phase;

2. During preparation, hydrogenated lecithin S-10, simple multifunctional emulsifier EMT-10 and Sibiricarb 305 are all in an oil phase;

3. During preparation, hydrogenated lecithin S-10 is added into a water phase, a simple multifunctional emulsifier EMT-10 and a Sibiricum 305 are added into an oil phase;

Under the above 3 conditions, the obtained frozen healing paste is uneven, not fine and coarse in different degrees, and no subsequent experiment is carried out.

Therefore, only when the hydrogenated lecithin S-10 is prepared by the preparation method, an oil phase is required to be added, and the emulsion matrix prepared by adding the simple multifunctional emulsifier EMT-10 and the Sibirk 305 into the water phase is more exquisite and uniform.

Example 7 (as a comparative example)

1. the dosage of the 30% triethanolamine solution is 0.4ml;

2. The dosage of the 30% triethanolamine solution is 1.3ml;

Both the above conditions result in a thinner system consistency of the prepared jelly-curing cream, and no subsequent experiments are carried out.

Example 8 (as a comparative example)

A preparation method of an anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing paste is similar to the example 1, and is characterized in that:

In the preparation of the step b), 0.4g of U30 cellulose thickener is firstly added into a container, 40.0mL of distilled water is added into the container, and as a result, the preparation speed of the U30 cellulose thickener is influenced, and no subsequent experiment is carried out.

Example 8 (as a comparative example)

1. Only hydrogenated lecithin S-10.35 g was used, and the simple multifunctional emulsifiers EMT-10 and Sibiricum 305 were not used (total amount of emulsifier was the same as in example 1);

2. only 0.35g of the simple multifunctional emulsifier EMT-10 was used, hydrogenated lecithin S-10 and Sibiricase 305 were not used (the total amount of emulsifier was the same as in example 1);

3. only 0.35g of Sibirch 305 was used, hydrogenated lecithin S-10 and simple multifunctional emulsifier EMT-10 were not used (total amount of emulsifier was the same as in example 1);

The three conditions lead to the prepared frozen cream to have rough textures with different degrees, not fine and smooth textures, can not meet the requirements, and are not subjected to subsequent experiments.

Example 9 (as a comparative example)

The dosage of hydrogenated lecithin S-10 is 3g, a simple multifunctional emulsifier EMT-10 and Saike 305 are not used, and the prepared jelly-curing cream is greasy and cannot meet the requirements, and no subsequent experiment is carried out.

Example 10

The physicochemical indexes of examples 1, 2, 3, and 4 are as follows:

10.1 traits

The jelly-curing cream prepared in the examples 1, 2 and 3 is bright yellow, has proper viscosity, is uniform and fine, has faint scent and has good spreadability (shown in figure 1). The jelly prepared in example 4 was bright yellow, viscous, with a pronounced grainy feel, slightly heavy coptis flavor, and poor spreadability, and example 4 was not subjected to further testing.

10.2PH check

A small amount of the jelly-curing cream prepared in examples 1, 2 and 3 is dipped by pH test paper, and the pH value is measured to be 6-7.

10.3 Cold and Hot test

The obtained cream was packaged in a transparent cosmetic bottle, and no delamination was observed when the cream was refrigerated in a refrigerator at 4℃for about one week, and a cold test was performed, and the results of example 2 are shown in FIG. 2. The mixture was placed in an incubator at 55℃for 24 hours, and a thermal test was conducted without the occurrence of delamination, off-flavor, etc., and the results of example 2 are shown in FIG. 3.

10.4 Centrifugal test

The obtained paste was packed in a tube with plug and centrifuged at 3000r/min for 20min, and no delamination was observed (the result of example 2 is shown in FIG. 4).

10.5 Room temperature Placement experiment

The cream prepared in examples 1, 2 and 3 was placed in a cosmetic bottle, and left standing at room temperature for 6 months, and no delamination phenomenon was observed, no change in feeling after use, and no change in smell were observed (the results in example 2 are shown in FIG. 5).

10.6 Irritation and allergy experiments

10.6.1 Experiments on irritation and allergy to Normal skin rats

The back of the rat was shaved, and the hair-shaved parts were respectively coated with the cream according to examples 1,2 and 3, and compared with the uncoated parts, and the results were not irritating and allergic, and the results of example 2 are shown in FIG. 6.

10.6.2 Irritation and allergy test on skin injured rats

6 SD rats were shaved on the backs, after the skin of the rats was scratched by a sterile blade, 1.5g of the cream was applied daily, and the applied sites were observed after 1 st, 3 rd and 5 th days of the application, and the skin of the rats was free from irritation and allergy, and the skin repairing effect was faster compared with the skin damaged rats without the cream applied, as shown in FIG. 7 of the results of example 2.

10.6.3 Test for irritation and allergy to a test person

The jelly prepared in the example was applied to the hand of volunteers (15-65 years old, 30 people) and observed after 30min, and no redness, eruption and foaming occurred, and the results of example 2 are shown in FIG. 8.

10.7 Inspection of the uniformity of the droplet size of the cream

A small amount of the cream is coated on a glass slide, covered with a cover slip, lightly pressed into a sheet with proper thickness, carefully observed under a microscope and visually observed on skin, and the cream drops of the cream are uniform in size, and the result of the example 2 is shown in figure 9.

10.8 Experiments on the permeability of the jelly

Taking agar powder, preparing 1% aqueous solution according to a proportion, and adding 8% ammonium iron sulfate solution as a color developing agent when the agar powder is in a liquefied state. Taking 2 test tubes, and adding the agar solution with proper amount. Mupirocin ointment and the frozen cream prepared in example 2 are respectively filled in a gap of 30mm at the upper end, and are uniformly mixed, and the height of a color zone is measured according to the corresponding time point. The square of the height of the color zone is taken as an ordinate, the time is taken as an abscissa, a straight line is fitted, and the slope of the color zone is obtained, namely the diffusion coefficient, and the penetration capacity of the three materials can be compared through the diffusion coefficient. The regression equations of mupirocin cream and jelly-healing cream are Y _{Row (Luo)} =0.0566x+0.5994 and Y _Freeze =0.52999x+1.4294, respectively, and as a result, the diffusion coefficients of mupirocin cream and jelly-healing cream are 0.5994 and 1.4294, respectively, and the jelly-healing cream is larger than the former, i.e. the permeability of the jelly-healing cream is better than that of Luo Piluo star ointment. Therefore, it can be primarily judged that the acting speed is faster than that of the ointment of lopirox, see fig. 10 and 11.

10.9 Investigation of anti-inflammatory Effect of the jelly-healing paste

10.9.1 Grouping, modeling and administration

The method comprises the steps of taking 24 male SD rats (the selected rats are basically the same in size and weight, and the weight difference is not more than +/-10 g) subjected to one week adaptation feeding, randomly dividing the rats into blank (KB), model (MX), low-freezing-curing-cream (DYGL) and high-freezing-curing-cream (DYGH) groups, removing hairs on the backs of the rats in the low-freezing-curing-cream group and the high-freezing-curing-cream group, and applying 0.05g (low-freezing-curing-cream group) and 0.15g (high-freezing-curing-cream group) of the low-freezing-curing-cream of the example 2 to the shaved areas of the rats for 7 days. At 7d, the blank group was subcutaneously injected with 0.1mL of physiological saline at the right hind paw of the rat, and the model group was subcutaneously injected with 0.1mL of 1% carrageenan solution (1% solution formulated with physiological saline) at the right hind paw of the rat. Both the low jelly (DYGL) and high jelly (DYGH) groups were subcutaneously injected with 0.1mL of 1% carrageenan solution at the right hind paw of the rat 0.5h post-dose to cause inflammation. Modeling success was shown to be significantly different for MX group foot swelling compared to KB group.

Effect of high and low concentration of 10.9.2 jelly on rat hind foot plantar swelling

As can be seen from fig. 12, after the carrageenan is molded, the MX group is significantly swollen compared with the KB group, which indicates that the carrageenan-induced rat foot swelling inflammation model is successfully molded, the swelling degree is significantly inferior to that of the MX group in DYGL and DYGH groups, and the effect of suppressing swelling is better than that of the DYGL group in DYGH group. It is expected that the high and low concentration of the jelly-curing cream has an inhibiting effect on inflammation of rat foot swelling caused by carrageenan.

Effect of 10.9.3 on foot swelling rate in foot-swollen rats

According to the above experiments, the swelling rate was counted on the thickness of the hind paw plantar foot measured using vernier calipers at the time of inflammation of 0, 0.5, 1.5, 2.0, 3.0 and 5.0 hours, respectively. Swelling ratio (%) = (plantar thickness at each time point after molding-thickness before molding)/thickness before molding×100%. Thickness before molding causes inflammation at 0h the thickness tested is shown in figure 13 to be significantly up-regulated (p < 0.01) for rat plantar swelling in MX group compared to KB group at various time points, indicating that carrageenan causes rat plantar swelling and molding was successful. The plantar swelling rates between 0.5 and 5.0h tended to decrease to a different extent (p <0.05 or p < 0.01) for both DYGL and DYGH groups compared to MX groups. The rate of plantar swelling was significantly reduced in DYGH groups compared to DYGL groups (p <0.05 or p <0.01, fig. 13). Fig. 13, p <0.01vs. kb group; ^#p<0.05,^## p <0.01vs.mx group; ^&p<0.05,^&& p <0.01vs. dcgl group; the gel can be preliminarily judged to have a certain inhibition effect on carrageenan-induced rat foot swelling, and has dose dependence.

Detection of NO content in 10.9.4 serum

After 5.0h of molding, the rats are weighed, a uratam solution (25%) is prepared according to the weight of 0.4mL/100g, the rats are anesthetized by intraperitoneal injection, eyeballs are removed for blood collection, the blood is centrifuged at high speed (4 ℃ at 3500r/min for 15 min), impurities such as blood cells are removed, and the supernatant is reserved as serum. The NO content of each group was determined using the NO kit according to the kit instructions. As can be seen from fig. 14, the NO content was significantly changed (p < 0.01) in MX group compared with KB group, indicating successful rat modeling. Compared with the MX group, the DYGL and DYGH groups can be extremely obviously reduced (p is less than 0.01), which indicates that the high and low dose groups of the jelly healing paste have certain anti-inflammatory activity, and the anti-inflammatory activity of the DYGH group is superior to that of the DYGL group and has dose dependency. Fig. 14, p <0.01vs. kb group; ^## p <0.01vs.mx group; ^$$ p <0.01vs. DYGL group.

Determination of MDA, GSH, SOD and CAT content in 10.9.5 rat serum

Serum prepared by 10.9.4 was taken, and the contents of MDA, GSH, T-SOD and CAT in each group were measured with oxidative stress kit (MDA kit, GSH kit, T-SOD kit and CAT kit), respectively, and whether the effect was dose-dependent was verified. As can be seen from fig. 15, MX group showed extremely significant up-regulation of MDA expression levels (p < 0.01) and extremely significant down-regulation of CAT, T-SOD and GSH expression levels (p < 0.01) compared to KB group, indicating successful modeling of carrageenan-induced inflammation. In contrast to MX group, MDA levels in serum of DYGL and DYGH groups were extremely significantly down-regulated (p < 0.01), and CAT, T-SOD and GSH expression levels were significantly up-regulated or extremely significantly up-regulated (p <0.05 or p < 0.01), indicating that the cream has anti-inflammatory activity. DYGL and DYGH showed significantly reduced MDA expression levels (p < 0.05), significantly increased levels of T-SOD and GSH expression (p < 0.05), and significantly increased CAT expression levels (p < 0.01), indicating that the high dose group had better anti-inflammatory activity than the low dose group, and was dose dependent. Fig. 15, p <0.01vs. kb group; ^#p<0.05,^## p <0.01vs.mx group; ^$p<0.05,^$$ p <0.01vs. DYGL group.

10.9.6 Elisa method for determining the content of cytokines IL-1 beta, IL-6, IL-10 and TNF-alpha in rat serum

Serum prepared by 10.9.4 is taken, and is completely thawed at room temperature by adopting an IL-1 beta ELISA kit, an IL-6ELISA kit, an IL-10ELISA kit and a TNF-alpha ELISA kit, and cytokines IL-1 beta, IL-6, IL-10 and TNF-alpha in the serum are detected by the Elisa kit. As can be seen from FIG. 16, the levels of IL-1β, IL-6 and TNF- α were all significantly increased in MX groups (p < 0.01) relative to KB groups; the extremely significant decrease in IL-10 content (p < 0.01) indicates successful modeling of the inflammatory model. The IL-1β, IL-6 and TNF- α levels in serum were all significantly reduced (p < 0.01) in DYGL and DYGH groups relative to MX groups; significant or extremely significant increases in IL-10 content (p <0.05 or p < 0.01). The IL-1β, IL-6 and TNF- α levels in the sera of group DYGH were all significantly or very significantly reduced (p <0.05 or p < 0.01) relative to group DYGL; the IL-10 content was significantly increased (p < 0.01). In summary, both DYGL and DYGH have some anti-inflammatory activity, and DYGH is better than DYGL, possibly dose-dependent. Fig. 16, p <0.01vs. kb group; ^## p <0.01vs.mx group; ^$p<0.05,^$$ p <0.01vs. DYGL group.

PGE ₂ content measurement in 10.9.7 rat inflammatory foot

The inflammatory foot is sequentially sheared at the same position above the ankle joint of each group of rats, soft tissues are sheared, weighed and sheared, the rats are fully immersed in 5mL of physiological saline for 1h, centrifuged at 3000rpm at 4 ℃,0.5 mL of supernatant is taken and uniformly mixed with 0.5mol/L KOH-methanol solution, the mixture is reacted in a water bath at 50 ℃ for 20min, the volume is fixed to 5mL by methanol, and the absorbance of the solution is measured at 278nm wavelength and expressed as absorbance per gram of inflammatory tissues. As can be seen from fig. 17, PGE ₂ content was significantly increased in MX group rats compared to KB group (p < 0.01); compared with the MX group, the PGE ₂ content of the DYGL and DYGH groups is extremely remarkably reduced (p < 0.01) after the effect of the frozen healing paste; the DYGH group had significantly reduced PGE ₂ content compared to DYGL group. Fig. 17, p <0.01vs. kb group; ^#p<0.05,^## p <0.01vs.mx group; ^$$ p <0.01vs. DYGL group.

10.10 Examination of itching-relieving Effect of the jelly

Another 24 SD rats were randomly divided into KB, MX, DYGL and DYGH groups of 6. The back of DYGL and DYGH groups were shaved, and the rats were evenly applied to the back dehairing area of DYGL (0.05 g of the cream prepared in example 2) and DYGH groups (0.15 g of the cream) once daily, to 7d. At 7d, KB was injected subcutaneously back with 0.6mL of saline and MX, DYGL and DYGH were injected subcutaneously back with 0.6mL of 8mg/mL histamine. The total number of itching in 20min was recorded in units of the number of times counted, the head was scratched by the front paw of the rat, the trunk was scratched by the rear paw, and the whole body was bitten by the mouth as an indication of itching. As a result, as shown in FIG. 18, the number of itching in the MX group was significantly increased compared to the KB group (p < 0.01). All 2 dose groups of the jelly-healing ointment can inhibit skin itch caused by histamine, the itch times are obviously reduced compared with that of MX group (p < 0.01), and the itch times of DYGH group are obviously reduced compared with that of DYGL group (p <0.01, figure 18). The frozen ointment can inhibit itching caused by histamine solution, has certain itching relieving effect, and has dose dependence. Fig. 18, p <0.01vs. kb group; ^## p <0.01vs.mx group; ^$$ p <0.01vs. DYGL group.

10.11 Experiments on the bacteriostatic Effect of the jelly

The jelly prepared in example 2 was selected as a subject. The bacteriostatic effect of the prepared jelly-curing ointment on staphylococcus aureus is measured by referring to a method specified in an annex C3 of GB 15979-2002. The results of the test bacteriostasis rate are shown in Table 1:

table 1 antibacterial effect of the jelly-curing ointment

Bacterial strain	Example 1	Example 2	Example 3
				Staphylococcus aureus	85.37％	83.26％	85.49％

The frozen healing paste has a certain antibacterial effect and can effectively prevent inflammation at chilblain as shown in table 1.

10.12 Examination of the comprehensive effects of the jelly

The study was approved by and conducted under the guidance of the ethical committee of the southern Anhui medical college. The efficacy of the jelly-care cream prepared in examples 1,2 and 3 was evaluated by using the trial feeling as follows. By using a poll evaluation method, 90 chilblain patients are selected as trial objects and divided into three groups at random, chilblain is treated by using the chilblain cream prepared in examples 1,2 and 3 respectively, and the chilblain is used for 3 times in the morning, in the middle and at the evening every day for 2 weeks (2020.02.02-2020.02.16). The total using effects are divided into 5 points: the score of 5 is the highest score, which shows good and very satisfactory; 4 is better; a score of 3 being acceptable; a score of 3 or less is not good and unacceptable. The average scores are as follows. The results are shown in Table 2:

Table 2 comprehensive Effect investigation

10.13 Clinical Effect investigation

30 Chilblain patients are from an outpatient clinic, and are aged 20-40 years, wherein 12 men and 18 women meet the diagnosis standard of chilblain (erythema nodosum period and erythema turgescence period). The skin-care cream is characterized by dark purple red or red plaque or induration at the positions of ears, cheeks, fingers, backs of hands, feet, heels and the like, has no ulcer, is tense and bright in surface, has low skin temperature when touched, can fade after pressure is removed, is slower to recover after pressure is removed, is self-perceived to have itching feeling, is burnt, is swollen and is aggravated after itching feeling is heated.

The treatment method comprises the following steps: the method comprises the steps of cleaning the uncrushed chilblain part with warm water at about 40 ℃, wiping the chilblain part, smearing a proper amount of the chilblain cream of the embodiment 2 on the chilblain part and the periphery by using a sterilizing cotton swab for 3 times/d, and each time in the morning, noon and evening, and repeatedly rubbing the chilblain part for half a minute before the application of the medicine to improve the blood circulation of the chilblain part, and keeping warm after the application of the medicine.

Evaluation of curative effect: and (3) curing: after treatment, red swelling, purple spots and induration disappear, no itching and pain feel is caused, and the skin is recovered to be normal; the method is effective: the red swelling and the purple spots are reduced, the induration is reduced, and the pain and the itching are obviously relieved after the treatment; invalidation: after treatment, red swelling, purple spots and induration are unchanged or the illness is aggravated, and itching and pain are unchanged or aggravated. Treatment results: after treatment, the therapeutic effect is shown in table 3.

TABLE 3 clinical chilblain treatment effect

Example number (example)	Number of cure cases (examples)	Number of effective cases (examples)
			30	27	3

In the 30 cases of treatment, the jelly-curing ointment has obvious curative effect on chilblain, wherein the cure rate is 90.0%, the effective rate is 10.00%, and the total effective rate is 100.0%. The soft-shelled turtle can be cured after being used for 2-3 days, and the severe-shelled turtle can be cured after being used for about one week.

For the 30 patients susceptible to chilblain, the proper amount of the chilblain curing cream of the example 2 is continuously used in winter for 2 times/d after one year, and the chilblain of 21 people does not recur in the whole winter and accounts for 70 percent; 5 people have chilblain recurrence, but the symptoms are much lighter than those of the previous year, accounting for 16.7% of the total number; the chilblain of 4 people still recurs, and the chilblain is almost the same as the previous year, and accounts for 13.3% of the total chilblain. Therefore, the jelly-curing cream has a certain effect of preventing chilblain recurrence.

In conclusion, the jelly-curing cream prepared by the invention has the effects of moisturizing, anti-inflammatory, detumescence, antipruritic, anti-cracking and repairing, deeply moisturizes skin, enhances the resistance of the skin to cold environment, can obviously improve chilblain of patients, effectively improves immunity, and has definite curative effect on chilblain prevention and treatment.

Claims

1. The anti-inflammatory, detumescent, antibacterial, antipruritic, anticracking and repairing jelly-curing cream is characterized by comprising the following components in parts by weight:

The calendula soaking oil is prepared by the following method:

Weighing appropriate amount of calendula flower petals, adding olive oil with 12-20 times of calendula flower petal mass, extracting at ultrasonic frequency of 20000-40000Hz for 0.5-1.5 hr, soaking at room temperature for 7-15 days, and filtering to obtain calendula soaking oil;

The preparation method of the anti-inflammatory, detumescence, bacteriostasis, itching relieving, anti-cracking and repairing jelly-curing cream comprises the following steps:

i) 0.1-1.0mL of cherry extract, 0.5-1.2mL of aizoon stonecrop herb extract, 0.1-1.3mL of spina gleditsiae extract, 0.3-1.0mL of Chinese fevervine extract, 0.1-1.5mL of watermelon peel extract, 0.5-1.3mL of waxberry extract, 0.1-0.3g of boric acid, 0.1-1.0g of reed rhizome gum, 0.001-0.005g of berberine, 0.1-0.3g of trehalose and 0.05-0.15g of total polysaccharide of Chinese fevervine are stirred and dissolved, and uniformly mixed to obtain solution A for standby;

2. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing cream according to claim 1, wherein the cherry extract is prepared by the following method:

removing the pit of fresh cherry, weighing a proper amount of pulp, putting into a juicer, adding distilled water 3-5 times of the cherry pulp, squeezing juice for 1.5-2.0min in a juicing mode, filtering to obtain filtrate, and performing suction filtration, wherein the volume of the filtrate is 2-3 times of the cherry pulp mL/g to obtain cherry squeezing liquid for later use;

The watermelon peel squeezing liquid is prepared by the following steps:

3. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing paste according to claim 1, wherein the aizoon stonecrop extract is prepared by the following method:

4. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-healing paste according to claim 1, wherein the spina gleditsiae extract is prepared by the following method:

5. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-healing paste according to claim 1, wherein the white extract of the chicken vector is prepared by the following method:

6. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing paste according to claim 1, wherein the waxberry extract is prepared by the following method:

7. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing cream according to claim 1, wherein the total polysaccharide is prepared by the following method:

weighing proper amount of and radix et rhizoma Rhei coarse powder, and extracting with water under reflux for 3 times: adding water 15-18 times of the mass of the coarse powder of the radix stephaniae tetrandrae for 15-45min, and extracting for 1.0-1.6h; adding water with the weight 9-13 times of that of the coarse powder of the radix stephaniae tetrandrae for 2 nd time, and extracting for 0.8-1.6h; adding water with the mass of 8-12 times of that of the coarse powder of the radix stephaniae tetrandrae for 3 rd time, and extracting for 0.4-1.2h; filtering each time, mixing filtrates, centrifuging at 4deg.C and 8000r/min for 10-25min, and concentrating clear supernatant to 10 times of the mass of radix et rhizoma Rhei coarse powder; and precipitating with 95% ethanol solution at a volume ratio of 1:7-1:2, standing overnight at 4deg.C, recovering the temperature to room temperature the next day, vacuum filtering, oven drying the filter cake, pulverizing, and sieving with 80 mesh sieve to obtain HEJI total polysaccharide.

8. The anti-inflammatory, detumescence, antibacterial, antipruritic, anti-cracking and repairing jelly-curing cream according to claim 1, wherein the essence is one or more selected from jasmine essence, lemon essence, lavender essence, rose essence, lily essence, melon essence or osmanthus essence.

9. A method for preparing the anti-inflammatory, detumescent, antibacterial, antipruritic, anticracking and repairing jelly cream according to any one of claims 1 to 6, which is characterized in that the method comprises the following steps: