KR101957411B1 - Cosmetic composition for improving skin conditions comprising herbal extracts-containing pectin microcapsule - Google Patents

Abstract

The present invention provides a cosmetic composition for improving skin conditions, comprising pectin microcapsules containing herbal medicine extracts, particularly Artemisiae argyi Folium and Cordyceps militaris extracts. The cosmetic composition of the present invention, due to structural stability of the pectin microcapsules, can stably maintain herbal medicine extracts, particularly the Artemisiae argyi Folium and Cordyceps militaris extracts contained therein. As a result, it is possible to enhance effects of the Artemisiae argyi Folium and Cordyceps militaris extracts in improving antioxidation, skin tone (whitening), and moisturizing effects and ameliorating wrinkles while a moisturizing effect, antioxidant effect and penetrating effect of the pectin microcapsules themselves are also expected, and thus the cosmetic composition of the present invention is expected to be widely used as a multifunctional cosmetic.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving skin comprising a herbal extract pectin microcapsule,

The present invention relates to a cosmetic composition for improving skin comprising a herbal extract pectin microcapsule, and more particularly to a cosmetic composition for skin moisturizing or wrinkle improvement comprising pectin microcapsules containing a lobule and a Chinese cabbage extract .

The consumption of anti-aging cosmetics that suppress skin aging or treat the symptoms of aging due to an increase in human life expectancy is increasing. However, most of the materials with various functions are unstable and the functions are not properly performed, which is a problem in the pharmaceuticals as well as the cosmetics field. Therefore, it is the most important issue to solve the above-mentioned safety problem and to increase the activity and the effect of the function of the skin by increasing the skin transferring power of the materials.

Research and development in the field of pharmaceuticals has been actively pursued in order to solve such a problem, but research and development in the field of cosmetics is insufficient. Thus far, liposomes, cyclodextrin complexes, multiple emulsions ) Have been applied, but stability still remains a problem.

The cosmetic manufacturing process is characterized by heating, physical stirring, and mixing of materials having different physico-chemical properties. Therefore, it is important to maintain the functionality, stability, and skin transfer power of the formulation during long-term storage and use. In recent years, natural polysaccharide-based formulation techniques have been developed which are natural products and exhibit excellent biocompatibility and effective functionalities, and at the same time, stable and economical. However, most of them are hyaluronic acid-based hydrogel manufacturing methods and formulation techniques. It has been registered.

In addition to the hydrogel based technique, a technique using cellulose for a natural polysaccharide-based formulation technique has also been developed and filed for an application for cellulose microparticles.

However, in the present invention, a cosmetic composition using microcapsules containing pectin having high viscosity and water solubility compared with cellulose, which is insoluble cellulose, is used.

Pectin is a polysaccharide mainly composed of galacturonic acid, an oxide of galactose. It is composed of alpha-1,4 bond of D-galacturonic acid unit. It is mainly distributed in the bark of plant fruit, Located in the middle layer. Pectin is useful as a structural support because intermolecular crosslinking occurs by calcium ions and strengthens the binding of cell walls due to this strong binding force. Therefore, in the case of producing microparticles for delivering cosmetic ingredients using pectin, it is possible to enhance the mechanical stability of the microparticles, thereby increasing the stability and efficacy of the cosmetic component, and at the same time, improving the antioxidative and moisturizing effects of pectin itself. Can be obtained.

Korean Patent Application No. 10-2007-0015864 Korean Patent Application No. 10-2012-0092853 Korean Patent Application No. 10-2012-0116400

In order to solve the above problems, the present invention is to provide a cosmetic composition for skin improvement comprising pectin microcapsules containing an extract of herbal medicine, especially a leaf and a Chinese cabbage extract. The cosmetic composition of the present invention can incorporate therein various functional materials having skin tone (whitening) improvement, wrinkle improvement, moisturizing and antioxidative functions by using a microparticle-based formulation technique of natural pectin, Safety and activity of the pectin microcapsule itself, and in addition, the moisture and penetration of the pectin microcapsule itself can be expected.

The present invention provides a cosmetic composition for improving skin comprising herbal extract pectin microcapsules.

In one embodiment, the herbal extract may be any one selected from the group consisting of lobules, Cordyceps, and mixtures thereof.

In one embodiment, the herbal extract is selected from the group consisting of water, methanol, ethanol, propanol, butanol, propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane And a mixture thereof. [0033] The term " a "

In one embodiment, the herbal extract-containing pectin microcapsule may consist of a core comprising the nanoparticles of herbal extracts and an oil, and an outer shell comprising pectin surrounding the core.

In one embodiment, the herbal extract-containing pectin microcapsules may be included in the range of 0.01 to 20% by weight of the total cosmetic composition.

In one embodiment, the skin-improving effect of the cosmetic composition may be at least one efficacy selected from the group consisting of skin moisturizing, antioxidant and wrinkle-improving.

In one embodiment, the cosmetic composition is selected from the group consisting of softening longevity, astringent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, serum, cleansing cream, cleansing foam, cleansing water, pack, body lotion, body cream, A make-up base, a foundation, and a body cleansing agent.

The present invention relates to a cosmetic composition comprising a herbal medicine extract in a pectin microcapsule, wherein the herbal medicine extract, in particular the ladybug and the Cordyceps sinensis extract, contained therein is stably maintained by the structural stability of the pectin microcapsule, (Whitening), wrinkle improvement, and moisturizing ability, and it is expected to be widely used as a multifunctional cosmetic because it has the moisturizing power and anti-oxidizing effect of pectin and oil contained in microcapsules.

Fig. 1A shows the crosslinking action by calcium ion in the pectin molecule, and Fig. 1B schematically shows the principle of formation of the pectin microcapsules containing the nanoparticles of the multifunctional component.
Figure 2A shows the three-dimensional structure of a fluid channel system for making pectin microcapsules, and Figure 2B shows a cross-section and size of a fluid channel system for making the pectin microcapsules of the present invention. The blue channel, yellow channel, and green channel of FIG. 2A represent the third flow channel 30, the second flow channel 20, and the first flow channel 10 of FIG. 2B, respectively.
Fig. 3 shows the microstructure and microstructure of pectin microcapsules containing nanoparticles of leaf and Cordyceps sinensis extracts.
Fig. 4 shows the antioxidative effect of the leaf extract (Fig. 4A), the leaf and the Chinese cabbage extract (Fig. 4B).
FIG. 5 shows the effect of increasing the expression of type 1 procollagen in the leaf extracts (FIG. 5A), the Cordyceps sinensis extract (FIG. 5B), the leaves and the Cordyceps sinensis extract (FIG. 5C).
Fig. 6 shows the expression of the wrinkle-related MMP gene and the inflammation-related COX-2 gene in the leaf extract (Fig. 6A) and the Cordyceps sinensis extract (Fig. 6B).
FIG. 7 shows the results of expression of HAS, FLG, and TGM-1 genes related to moisturization of the leaf extract (FIG. 6A) and the Chinese cabbage extract (FIG. 6B).
Fig. 8 shows the effect of suppressing the production of nitric oxide (NO) by the leaf extract (Fig. 6A) and the Chinese cabbage extract (Fig. 6B).
FIG. 9 shows a process for producing a cosmetic lotion containing the lavender and Cordyceps sinensis extract pectin microcapsule composition of the present invention.
10 is a graph showing skin moisturization improving effects (Figs. 10A and 10B) and skin tone improving effect (Figs. 10C and 10D) of lotion pectin microcapsule composition of the present invention containing a lavender and a Chinese cabbage extract.
Fig. 11 shows a process for producing a serum comprising the microcapsule and the Cordyceps sinensis extract of the present invention.
12 is a graph showing the skin moisturizing effect (Figs. 12A and 12B) and the skin tone improving effect (Figs. 12C and 12D) of the lotion containing the safflower and the Cordyceps sinensis extract-containing capsule pectin microcapsule composition of the present invention.
FIG. 13 shows a process for producing a cream containing the lobule and Cordyceps sinensis extract pectin microcapsule composition of the present invention.
14 is a graph showing the skin moisturizing effect (Figs. 14A and 14B) and the skin tone improving effect (Figs. 14C and 14D) of lotion pectin microcapsule composition of the present invention containing the epilator and Cordycepsae extract.
Fig. 15 shows an example of the effect of improving the moisturizing effect of lotion pectin microcapsules (Fig. 15A), serum (Fig. 15B) and cream (Fig. 15C) containing herbal medicine extract of the present invention.
Fig. 16 is an exemplary photograph showing the skin tone improvement effect of lotion pectin microcapsules (Fig. 16A), serum (Fig. 16B) and cream (Fig. 16C) containing herbal medicine extract of the present invention.
17 shows a photograph of a prototype of a lotion, serum and cream using the herbal medicine extract pectin microcapsule of the present invention.

The present invention provides a cosmetic composition for improving skin comprising herbal extract pectin microcapsules.

In one embodiment, the herbal extract may be any extract selected from the group consisting of lobules, cordyceps, and mixtures thereof, but is not limited thereto.

The lobe may be at least one selected from the group consisting of yellow mugwort, mugwort, lepidoptera, and mugwort, but is not limited thereto. It is used in the treatment of physiological impairment and gynecological diseases because it warms the blood of 气血 and gyeongmaek (经脉). It has pharmacological effects such as hemostatic action, antibacterial action, bronchial smooth muscle relaxation action, Action, and hypersensitivity shock protection. The main component of the leaves is chinol, which accounts for 50%, and contains athenin, choline, tannin and chlorophyll. In particular, the chineol component is known to have a function of promoting the circulation of heat and blood circulation.

The caterpillar fungus may be, but is not limited to, a pupa caterpillar or a natural caterpillar larva caterpillar. The caterpillar fungus is a form that is mostly parasitic to an insect but forms a fruiting body after insect death. It is a name given to mean that in winter, the insect became a mushroom in summer. Analysis of the components of Cordyceps sinensis reveals that 11 kinds of amino acids and 10 kinds of amino acids, which are necessary for human body, occupy more than 60% of the total content and contain about 7% of cordycepin. In addition, linolenic acid, an antioxidant lipid necessary for cell activation, and 3'-deoxyadenosine, a hexane, are contained in a considerable amount. In addition, various pharmacological actions such as immunity enhancement are known.

The pectin microcapsule of the present invention can stabilize various components contained therein to maintain its activity, so that it is possible to use various natural materials or oriental herb extracts, such as red ginseng, casualty Extracts such as corn oil and thistle, vermicelli (coenzyme Q10), persicaria. Hydropiper, Berberis koreana bark, ginseng, mushroom (mugwort, . In addition, cosmetic functionalities can be enhanced by introducing various active ingredients commonly used in the cosmetics industry such as coenzyme, EGF, flavonoid, vitamin, kojic acid, niacinamide, curcumin, and saponin.

In one embodiment, the herbal extract is selected from the group consisting of water, methanol, ethanol, propanol, butanol, propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane And a mixture thereof, and is preferably an ethanol extract.

The suitable amount of the extraction solvent is about 1 to 10 times, more preferably 5 to 8 times, and most preferably 5 times, based on the combined dry weight of the leaf and cordyceps. As the extraction method, it is possible to use heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction and the like, and they can be extracted once or repeatedly. The extraction temperature is not particularly limited so long as the effective activity of the active ingredients of the leaf and cordyceps is not removed, but is preferably extracted by immersion at room temperature.

The concentration of the ethanol may be 40 to 70%, but it is not limited thereto, and it can be appropriately adjusted according to the weight, temperature and desired components of the leaf and cordyceps.

In one embodiment, the herbal extract pectin microcapsule may comprise a core comprising the nanoparticles of herbal extracts and an oil, and an outer shell comprising pectin surrounding the core (see FIG. 3).

Pectin is a complex polysaccharide composed of methyl ester, an α- (1,4) glycoside bond of D-galacturonic acid units found in plant cell walls, and is a biocompatible, non-toxic coating material. Pectin is also known to have moisturizing and antioxidant properties. When microparticles for delivering cosmetic ingredients are prepared using pectin, the mechanical stability of the microparticles due to intermolecular crosslinking is enhanced by calcium ion, thereby maintaining the activity of the active ingredient of the cosmetic, and the moisturizing and antioxidant effect of the pectin itself can be obtained Functional fine particles can be produced.

The herbal extract pectin microcapsule of the present invention enables the oil phase to be wrapped inside the pectin through precipitation of herbal medicine extract with ethanol and cross-linking ionic bond between carboxyl group of pectin and divalent calcium ion (see FIG. 1).

In one embodiment, the crude herbal extract pectin microcapsules are prepared by mixing (a) an herbal medicine extract with an oil at a ratio of 1: 100 (w: v) and sonicating at 60 ° C for 2 hours to obtain a nano- Preparing a particle solution; (b) mixing the pectin aqueous solution obtained by stirring and dissolving the nanoparticle solution of step (a), 1 wt% of pectin powder and 1 wt% of polyoxyethylene sorbitan monooleate in water for 2 hours, 1 mixture; (c) mixing the first mixture of step (b) with ethanol, water and glycerin in a volume ratio of 40:40:20, and further adding 1 g of CaCl ₂ per 100 ml of the mixture to obtain a glycerin solution Mixed to give a diameter of 20 to 500 nm Preparing pectin microcapsules; And (d) separating and drying the pectin microcapsules prepared in step (c), wherein steps (a) to (c) are performed using a fluid channel system, A first flow channel (10) for supplying a particle solution; A second flow channel (20) disposed to surround the first flow channel and supplying a pectin aqueous solution; A third flow channel (30) disposed to surround the second flow channel and supplying a glycerine solution; A bottleneck structure (40) in which the first flow channel and the second flow channel meet; A first reaction channel 50 extending from the bottleneck structure 40; And a second reaction channel (60) connected to the first reaction channel (50) and the third flow channel (30), wherein i) the supply of nanoparticle solution in the first flow channel The flow rate Q _oil is 0.1 to 1.0 μl / min; (ii) the feed flow rate (Q _pec ) of the pectin aqueous solution in the second flow channel is 1 to 5 μl / min; And iii) the feed flow rate (Q _gy ) of the glycerin solution in the third flow channel is 10 to 50 μl / min (see FIGS. 2A and 2B).

In step (a), nanoparticles of a functional ingredient that can be stably contained in the oil phase in the pectin microcapsules can be prepared. The ethanol extract of herbal medicine is added dropwise to the oil while ultrasonication. Functional components with high polarity can be precipitated with a drastic decrease in solubility in apolar oil and ethanol can be removed by evaporation by ultrasonication at a relatively mild temperature of 60 ° C. As a result, the nanoparticles of the functional component are obtained in a state of being dispersed in the oil. The dispersion can be used as an internal core liquid phase in a fluid channel system in that the surfactant is added without addition and the particle diameter is nanoscale. The oil component is added in order to enhance the moisturizing effect and stabilize the herbal ingredients. For example, mineral oil, castor oil, avocado oil, peppermint oil and the like can be used, but mineral oil is preferably used.

In step (c), in the present invention, a calcium ion aqueous solution and an ethanol mixed solution are applied to a fluid channel system so that precipitation by ethanol and crosslinking by calcium ions enable the oil phase to be wrapped inside the pectin. Also, water permeability is increased without interfering with the flow of the microfluid by including a water-soluble substance as nanoparticles on the internal oil. However, in the present invention, pectin microcapsules containing not only hydrophobic substances but also hydrophilic substances are prepared in the present invention, and the stability and activity of the herbal medicine component And the skin transfer efficiency was improved.

Supply flow rate (Q _oil) of the oil and nano-particle solution, wherein the supply of the pectin solution in the second flow channel a flow rate (Q _pec), and the supply flow rate of the glycerine solution in the third flow channel (Q _gly) An oil emulsion is formed in the first flow channel section 50 where the oil and the functional nanoparticles meet the pectin aqueous solution and the microcapsules 50 are formed in the second flow channel 60 where the glycerin solution joins, .

In one embodiment, the herbal extract pectin microcapsule may comprise 0.01 to 20% by weight, preferably 1 to 5% by weight of the total cosmetic composition, and if it is less than 0.001% by weight, the efficacy of the cosmetic composition may not be exhibited If it is more than 20% by weight, the stability of the formulation may be deteriorated.

In one embodiment, the cosmetic composition may further comprise, but is not limited to, compositions selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymeric peptides, polymeric polysaccharides, and sphingolipids.

In one embodiment, the cosmetic composition may further comprise one or more additives selected from the group consisting of a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, an antioxidant, a plant extract, But are not limited to, adjuvants incorporated into cosmetics such as perfumes, blood circulation accelerators, cold agents, antiperspirants, and purified water.

Examples of the sustaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention, and plant preservation.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester. Do not.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the bactericide include hinokitiol, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, photosensitizer 301 But are not limited to, sodium mononitroguaiacol, undecylenic acid, and the like.

Examples of the antioxidant include, but are not limited to, butylhydroxyanisole, gallic acid propyl, and erythorbic acid.

Examples of the pH adjuster include, but are not limited to, citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include, but are not limited to, higher alcohols such as cetyl alcohol.

The above-mentioned compounding ingredients may be blended within the range not to impair the objects and effects of the present invention, but they are blended in an amount of 0.01-5% by weight, preferably 0.01-3% by weight based on the total weight.

In one embodiment, the skin improving effect of the cosmetic composition may be at least one efficacy selected from the group consisting of skin moisturizing, antioxidant and wrinkle improving, and preferably has a skin moisturizing effect. However, the present invention is not limited thereto, and it may further have a whitening, anti-inflammatory, antibacterial action.

The cosmetic composition of the present invention has antioxidation, moisturizing effect and penetration power of pectin microcapsules in addition to antioxidation, wrinkle improvement, whitening, moisturizing and anti-inflammatory effects of the leaf and cordyceps extract, .

In one embodiment, the cosmetic composition is selected from the group consisting of softening longevity, astringent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, serum, cleansing cream, cleansing foam, cleansing water, pack, body lotion, body cream, The composition may be prepared into one type of formulation selected from the group consisting of essence, makeup base, foundation, and body cleansing agent, and preferably one type of formulation selected from the group consisting of serum, lotion and cream, It does not.

Hereinafter, the present invention will be described in more detail with reference to Examples. The present embodiments are intended to clearly illustrate the technical features of the present invention and do not limit the scope of the present invention.

<Examples>

Example 1: Preparation of Cordyceps mellifera and lobster extract

The herbal medicine extract of this example was prepared according to the method described in Korean Patent Application No. 10-2017-0111958. That is, the dry liquor and the Cordyceps sinensis powder (Mushtech Co., Ltd.) were mixed at a weight ratio of 2: 1, and then 7 to 8 times as much as the dry weight of the mixture was added. Day. The extract was concentrated to a Modul spin drying and concentration system (Biotron Corporation) at 40 占 폚 for 24 hours. While stirring the concentrated extract, purified water of about 10 times the weight of the concentrated extract was added dropwise, and the mixture was allowed to stand at 5 캜 for 30 minutes, followed by vacuum filtration to obtain a precipitated powder.

Example  2: Cordyceps and Lady  Production of complex functional nanoparticles of extract

In the present invention, a nanoparticle solution containing the leaf extract and ethanol extract of Cordyceps sinensis was used as the functional ingredient. First, 50 mg of each of the obtained leaf and cordyceps extract powder was dissolved in 2 ml of ethanol and 0.2 ml of a leaf-ethanol solution was added dropwise to 10 ml of mineral oil while ultrasonication was carried out at 60 ° C for 2 hours. The nanoparticles of Cordyceps were prepared. The ultrasonic treatment was continued until the ethanol on the mineral oil was volatilized and became a clear solution.

In order to confirm whether the nanoparticles of the herbal medicine extract in the pectin microcapsules were added, a luminescent fluorescent dye (fluorescein) was mixed with the leaf lance and Cordyceps extracts in the production of the nanoparticles. As can be seen from the fluorescence image in FIG. 3, it was confirmed that red fluorescence was expressed in the mineral oil core portion in the pectin capsule, and red fluorescence was expressed by the complex of the nanoparticles of the leaf and cordyceps with the fluorescent dye It can be confirmed that the nanoparticles of the leaves and the Cordyceps sinensis extract are stably contained in the pectin capsules.

Example 3: Preparation of Cordyceps and Leaf extract hampo pectin microcapsules

1. Structure of fluid channel system

The pectin microcapsules of the present invention were prepared by a fluid channel system by the method described in Korean Patent Application No. 10-2017-0070910, and the fluid channel system was prepared by a soft lithography method using PDMS (polydimethylsiloxane) Lt; / RTI &gt; Its structure is shown in Figures 2A and 2B. Specifically, the fluid channel system comprises a first flow channel 10 (green-colored channel of FIG. 3) for supplying the mineral oil and nanoparticle solution; A second flow channel (20, yellow channel in Figure 3) disposed to surround the first flow channel and supplying a pectin aqueous solution; A third flow channel (30, purple channel of Figure 3) disposed to surround the second flow channel and supplying a glycerin solution; A bottleneck structure (40) formed in such a manner that the first flow channel and the second flow channel meet and gradually expand from the meeting point to the opposite side; A core shell connected to the first flow channel 10 and the second flow channel 20 from the bottleneck structure 40 and forming a core shell in which the mineral oil, the nanoparticle-containing solution, and the pectin aqueous solution are mixed A first reaction channel (50); And a second reaction channel (60) connected to the first reaction channel (50) and the third flow channel (30) to form microcapsules having the core shell and the pectin shell outer shell.

2. Selection of Microcapsule Manufacturing Conditions in Fluid Channel System

The flow rate (Q _oil / Q _pec ) of the mineral oil and the pectin solution was adjusted to less than 0.5 μl / min so as to form a mineral oil emulsion in the first channel section, and then the flow rate (Q _gly ) of the glycerin solution was adjusted to 20 μl / min Pectin &lt; / RTI &gt; microcapsules were successfully prepared.

3. Manufacture of microcapsules using fluid channel system

(1) Powdered pectin and Tween 80 were mixed in water at a concentration of 1 wt%, respectively, and stirred for 2 to 3 hours to completely dissolve the pectin aqueous solution. In addition, ethanol, water and glycerin were mixed at a volume ratio of 4: 4: 2 to prepare a mixed solution of 40% ethanol, 40% water and 20% glycerin. 1 wt% of calcium chloride (CaCl ₂ ) was added to the mixed solution and stirred for 1 hour to dissolve completely to prepare a glycerin solution. The fabricated fluid channel system was then placed in a glass Petri dish containing 40% ethanol, 40% water and 20% glycerin solution in which 1 wt% calcium chloride was dissolved. Each prepared solution was injected into each channel using a syringe pump (model: KDS-101) as follows. Mineral oil and nanoparticle solutions were injected into the first flow path at a flow rate (Q _oil ) of 0.1-1.0 μl / min, pectin and tween 80 solutions were flowed at a flow rate (Q _pec ) of 2-5 μl / And a mixed solution of 40% ethanol, 40% water and 20% glycerin containing 1 wt% of calcium chloride was injected into the third flow channel at a flow rate ( _Qgly ) of 10 to 40 ㎕ / min, Of pectin microcapsule structure was prepared.

(2) In the above flow rate condition, a mineral oil emulsion is formed in the first flow channel section 50 where the mineral oil and the functional nanoparticles meet the pectin aqueous solution, and in the second flow channel 60 section where the glycerin solution joins Microcapsules were formed through gelation of pectin.

Example  4: Lady  And the production of Cordyceps sinensis extract capsule pectin microcapsule lotion

A phase weight% B phase weight% Purified water 82.2240 Purified water 2.0000 Dipropylene glycol 5.0000 Carbomer 0.1600 Hydroxyethylurea 3.0000 C phase weight% glycerin 2.9680 PEG-40 Hydrogenated Castor Oil 0.1500 Niacinamide 2.0000 PPG-26-Butes-26 (PPG-26-Buteth-26) 0.1500 Benzyl glycol 0.7500 Spices 0.0500 Betaine 0.5000 D phase weight% PEG-32 0.5000 Purified water 1.0000 Propanediol 0.4000 Tromethamine 0.1200 Butoxydiglycol 0.2000 E phase weight% Allantoin 0.2000 Leaf and Cordyceps extract 0.1000 Panthenol 0.2000 ethanol 0.0350 Glyceryl polyacrylate 0.1000 Oleic acid 0.0018 Dipotassium glycyrrhizate 0.0500 Calcium chloride 0.0009 Raspberry ketone 0.0500 pectin 0.0002 Adenosine 0.0400 Polysorbate 80 Component 0.0001 Disodium EDTA 0.0200 Hydrolyzed
Glycosaminoglycans 0.0500 Xanthan gum 0.0200

First, the components of the A phase shown in Table 1 were put into an aqueous dissolution tank and dissolved by heating at 55 DEG C. Then, the ingredients of the B phase were added and the mixture was completely dispersed at room temperature. The components of the solubilized part are put into a separate container and the ingredients of the C phase are charged, and then heated to 50 ° C to be completely dissolved. Add A and B phases to the main mixer and homogenize for 5 minutes with Disper 1000 rpm. Stir the C phase as the solubilized part slowly and stir for 5 minutes under the same conditions. Add purified water and tromethamine in a separate container and thoroughly dissolve by thoroughly stirring at room temperature. Add D phase as a neutralizer to the main mixer. And then homogenized by stirring for 10 minutes under the same conditions. As an additive part, the E phase of the leaf and the Chinese cabbage pectin microcapsule extract was added, and the mixture was cooled to 30 캜 and stirred. After the quality inspection, it is discharged and stored (refer to FIG. 9). In Table 1, the components and composition ratios of the E phase are shown in terms of the weight of the entire cosmetic composition in terms of the components used in the production of the lobe and Cordyceps sinensis extract pectin microcapsules. In the process of Example 4, And the extract of Cordyceps sinensis extract capsule pectin microcapsules can be used in the production of cosmetics in an amount of 0.01 to 20% by weight of the total cosmetic composition.

Example  5: Lady  And Cordyceps sinensis extract Shotgun pectin microcapsules Serum  Produce

A phase weight% B phase weight% Purified water 61.79616 Caprylylmethicone 4.00000
Dipropylene glycol
5.00000 PEG-12 dimethicone / PPG-20
Cross polymer
1.00000 glycerin 4.41000 Spices 0.05000 Methylglucose-20 3.00000 Niacinamide 2.00000 C phase weight% Betaine 1.00000 Purified water 12.00000 Benzyl glycol 0.75000 Carbomer 0.24000 Panthenol 0.50000 Propanediol 0.40000 D phase weight% Butoxydiglycol 0.20000 Purified water 2.00000 Xanthan gum 0.15000 Tromethamine 0.18000 Allantoin 0.10000 Raspberry ketone 0.05000 E phase weight% Adenosine 0.04000 ethanol 0.70000 Sodium hyaluronate 0.03000 Leaf and Cordyceps extract 0.10004 Phenoxyethanol 0.02400 Oleic acid 0.03600 Disodium EDTA 0.02000 Calcium chloride 0.01800 Butylene glycol 0.15000 pectin 0.00340 Hydrolyzed
Glycosaminoglycans 0.05000 Polysorbate 80 0.00240

First, the ingredients of the A phase shown in Table 2 were added to an aqueous dissolution tank and dissolved by heating at 55 DEG C, and then the components of the B phase were completely dispersed at room temperature in the oily dissolution tank. Components of the C phase are put into a gum mixer and prepared by completely dispersing at room temperature. Add the A-phase and gum mixer to the main mixer, and stir for 5 minutes at a Disper 1000rpm. After the homogeneous stirring is completed, the B phase is added, and the mixture is stirred at 30 rpm for 5 minutes using only a paddle mixer to uniformly disperse the oil capsules. Add D phase components into a separate vessel and dissolve them into the main mixer, and then stir at 30 rpm for 5 minutes using only the paddle mixer. After adding ingredients of the additive filler E, the mixture was stirred for 5 minutes at 30 rpm in a paddle mixer, cooled to 30 캜, defoamed, and quality controlled and stored (see FIG. 11). In Table 2, the ingredients and the composition ratios of the E phase are shown in terms of the weight of the total cosmetic composition based on the components used in the preparation of the lobe and Cordyceps sinensis extract pectin microcapsules. In the process of Example 5, And the extract of Cordyceps sinensis extract pectin microcapsules can be used in the production of cosmetics in an amount of 0.01 to 20% by weight of the total cosmetic composition.

Example  6: Lady  And Cordyceps sinensis Extract Capsule Pectin Microcapsule Cream

A phase weight% B phase weight% Purified water 54.3606 Cetearyl alcohol 3.0000 Dipropylene glycol 6.0000 Hydrogenated
Coconut oil 2.0000 Tripropylene glycol 4.0000 Cetearyl glucoside 1.3750 Niacinamide 2.0000 Glyceryl stearate 1.3000 Betaine 2.0000 Sorbitan olivate 0.6250 glycerin 1.4900 Benzyl glycol 0.7500 C phase weight% Panthenol 0.5000 Purified water 14.0000 Propanediol 0.4000 Carbomer 0.2000 Butoxydiglycol 0.2000 Acrylate / C10-30 alkyl acrylate
Cross polymer 0.0800 Allantoin 0.1000 E phase weight% Raspberry ketone 0.0500 ethanol 2.8000 Hydrolyzed
Glycosaminoglycans 0.0500 Leaf and Cordyceps extract 0.2002 Adenosine 0.0400 Oleic acid 0.1440 Disodium EDTA 0.0200 Spices 0.1000 D phase weight% Calcium chloride 0.0720 Purified water 2.0000 pectin 0.0136 Tromethamine 0.1200 Polysorbate 80 0.0096

First, the ingredients of the A phase shown in Table 3 were put into an aqueous dissolution tank and dissolved by heating at 75 DEG C, and the B phase was dissolved in an oil phase dissolving tank at 75 DEG C. Prepare the C phase in a gum mixer by completely dissolving it at room temperature. The A-phase and the gum-mixer are put into the main mixer, and the mixture is homogeneously stirred, and the B-phase is slowly added while stirring. After completion of the addition, the homomixer is stirred at 2000 rpm and the paddle mixer is kept at 25 rpm for 5 minutes. D phase is put in a separate container, thoroughly stirred and fully dissolved, and then charged into the main mixer. After completion of the addition, the mixture is agitated for 5 minutes at HM 2000rpm and PM 25rpm. After the temperature of the main mixer is cooled to 50 캜, the E phase, which is the additive part, is added and mixed for 3 minutes under the same conditions. Deg.] C to 30 [deg.] C, and quality control is carried out (see Fig. 13). In Table 3, the ingredients and composition ratio of the E phase are shown in terms of the weight of the total cosmetic composition based on the components used in the preparation of the lobule and Cordyceps sinensis extract pectin microcapsules. In the process of Example 6, And the extract of Cordyceps sinensis extract capsule pectin microcapsules can be used in the production of cosmetics in an amount of 0.01 to 20% by weight of the total cosmetic composition.

<Experimental Example>

Experimental Example  1: Cordyceps and Lady  Evaluation of efficacy of extract

(1) Evaluation of antioxidant activity: Measurement of free radical scavenging activity using DPPH

Ethanol-soluble DPPH solution was dispensed into a test tube in an amount of 495 占 퐇, and 5 占 퐇 of the leaf and Cordyceps extracts were added thereto to give a final reaction solution of 500 占 퐇. After reacting for a predetermined time, 200 [mu] l of the solution was dispensed into two wells of a 96-well plate, and the change in absorbance at 517 nm was measured.

(2) Wrinkle improvement evaluation: 1 type procollagen gene expression investigation

A fibroblast division such that the 5 × 10 ⁵ cells / well of the cell lines NIH-3t3 cells in 6-well plates, and remove the culture medium and rinsed with PBS. PBS was dispensed at a rate of 1 ml / well, and UVB was irradiated with UVB at a dose of 30 mJ / cm 2 using an ultraviolet irradiation apparatus (Vilbert Lourmat). After irradiating ultraviolet rays, PBS was removed and replaced with fresh medium, and then the leaf and cordyceps extracts were treated and allowed to stand for 24 hours. The total RNA was extracted by treatment with a trizol reagent, cDNA was synthesized from the extracted RNA, and PCR was performed to comparatively measure the expression of the type 1 procollagen gene. The PCR conditions were 95 ° C for 5 minutes, denaturation at 95 ° C for 30 seconds, annealing at 55 ° C to 60 ° C for 1 minute, extension at 72 ° C for 1 minute for 30 cycles, and further reaction at 72 ° C for 5 minutes. Each PCR product was electrophoresed on a 1.5% agarose gel containing EDTA (ethidium bromide) to confirm expression of type 1 procollagen gene. As the PCR primers used, a sense primer 5'-AGCCAGCAGATCGAGAACAT-3 'and an antisense primer 5'-TCTTGTCCTTGGGGTTCTTG-3' were used.

(3) Wrinkle improvement evaluation: Wrinkle related gene expression investigation

The epithelial cell line HaCaT cells were added to DMEM medium containing 10% fetal bovine serum (FBS), 100 units / ml penicillin, and 100 μg / ml streptomycin and cultured in a 5% CO ₂ air-mixed incubator at 37 ° C . After cultivation, HaCaT cells in 6-well plate at 1 × 10 ⁶ was dispensed such that the cells / well. After 1 hour of preliminary treatment with the extracts of the next day (25, 50, 100 占 퐂 / ml) and Cordyceps sinensis extract (12.5, 25, 50 占 퐂 / ml) Respectively. After irradiation with UV light, the leaf extract (25, 50, 100 ㎍ / ㎖) and Cordyceps sinensis extract (12.5, 25, 50 ㎍ / ㎖) were treated and incubated for 24 hours. After culturing, RNA was extracted and confirmed by RT-PCR for the photoengineering genes MMP (metallomatrix protease) -1,2,9 and COX-2, and the housekeeping gene GAPDH (Glyceraldehyde 3-phosphate dehydrogenase). PCR amplification is i-Master PCR using the kit (iNtRON), each group of sense and antisense primers and cDNA target protein, GAPDH control primers and 250uM dNTP, Tris-HCl (pH 8.3) 10mM, 50mM KCl, 1.5mM ₂ NgCl Of HiPi solution. The PCR conditions were 95 ° C for 5 minutes, denaturation at 95 ° C for 30 seconds, annealing at 55 ° C to 60 ° C for 1 minute, extension at 72 ° C for 1 minute for 30 cycles, and further reaction at 72 ° C for 5 minutes. The primers used were as shown in Table 4 below.

Target gene The nucleotide sequence (5 → 3) MMP-1 Sense TCT GAC GTT GAT CCC AGA GAG CAG
Antisense CAG GGT GAC ACC AGT GAC TGC AC MMP-2 Sense CAA GTG GAG AGC AGT TGA GGA CAT C
Antisense TGA GGA CAT CTC CCA CGT CAA MMP-9 Sense TCC CTG GAG ACC TGA GAA CC
Antisense GGC AAG TCT TCC GAG TAG TTT COX-2 Sense CAA AAG CTG GGA AGC CTT CT
Antisense CCA TCC TTC AAA AGG CGC AG GAPDH Sense CAA TGA ATA CGG CTA CAG CA
Antisense AGG GAG ATG CTC AGT GTT GG

(4) Evaluation of anti-inflammatory effect: Nitric oxide (NO) production

The macrophage cell line RAW 264.7 was adjusted to a concentration of 1 × 10 ⁶ cells / ml using RPMI 1640 medium containing penicillin (100 IU / ml), streptomycin and 10% FBS, and then inoculated in a 96-well plate , Preincubated for 18 hours at 5% CO ₂ and 37 ° C. After that, the medium was removed, and 50 쨉 l of the leaf and cordifolium extract prepared at a concentration of 4 times and a medium containing 50 쨉 L of LPS (final concentration 1 / / ml) were simultaneously treated and cultured. After 24 hours, the supernatant was transferred to another 96-well plate in an amount of 100 쨉 l each, and the solution was added with a Griess solution (0.5% naphthylethylenediamine dihydrochloride, 5% sulfanilamide, 25% H ₃ PO _4] was subjected to quantification using NO. Calibration curves were prepared using sodium nitrite (0-100 [mu] M) as a standard.

(5) Evaluation of moisturizing effect:

In order to investigate the degree of expression of the HAS-1 to HAS-3, TGM-1 and FLG genes related to moisturization by the extracts of Cordyceps militaris and Cordyceps sinensis extracts at the transcription level, the epidermal cell line (HaCaT) 100, 200 占 퐂 / ml) and retinol (final concentration 10 占 퐂 / ml) for 24 hours. Total RNA was extracted using a trizol reagent. The extracted total RNA was prepared by using a first strand cDNA synthesis kit (Thermo scientific), and then the same amount of cDNA was amplified by PCR. The nucleotide sequences of the sense and antisense primers of the above used genes are shown in Table 5, and GAPDH was used as a control gene. PCR amplification was carried out by using a 2x PCRBIOHS Taq premix kit (PCRBIO), a sense and antisense primer of each test group cDNA and target proteins, a control GAPDH primer, dNTP 2 mM, MgCl ₂ 6 mM, HS Taq DNA polymerase And 10 μl of the PCR premix kit. The PCR conditions were 30 cycles of denaturation at 95 ° C for 10 seconds, annealing at 58 ° C for 15 seconds, and extension at 72 ° C for 1 minute.

Target gene The base sequence (5 '- &gt; 3') FLG Sense AGG GAA GAT CCA AGA GCCCA
Antisense ACT CTG GAT CCC CTA CGC TT TGM Sense GAA ATG CGG CAG ATG ACG AC
Antisense AAC TCC CCA GCG TCT GAT TG HAS-1 Sense CCA CCC AGT ACA GCG TCA AC
Antisense CAT GGT GCT TCT GTC GCT CT HAS-2 Sense TTC TTT ATG TGA CTC ATC TGT CTC ACC GG
Antisense ATT GTT GGC TAC CAG TTT ATC CAA ACG HAS-3 Sense TAT ACC GCG CGC TCC AA
Antisense GCC ACT CCC GGA AGT AAG ACT GAPDH Sense CAA TGA ATA CGG CTA CAG CA
Antisense AGG GAG ATG CTC AGT GTT GG

Experimental Example 2: Evaluation of skin irritation

(1) Test method

A total of 31 subjects underwent a skin patch test using a Finn chamber. The back portion of the subject was wiped with 70% ethanol and dried. Then, 20 μl of the test substance was dropped and fixed on the test site. In the case of the liquid type test material, the filter paper disk was placed in a pin chamber having a diameter of 8 mm, and then 20 μl of the test substance was dropped and fixed on the test site. The patches were attached for 24 hours. After 30 minutes, 24 hours, and 48 hours after removing the patches, the dermatologist examined the degree of stimulation according to the criteria of the International Contact Dermatitis Research Group (ICDRG).

(2) Evaluation of adverse reaction

The person in charge of the test should be informed of the presence of skin erythema, edema, scaling, itching, stinging, burning, tightness, prickling, And the degree of skin anomalies was displayed and the results were drawn. In addition, we surveyed the subjects about skin adverse reactions.

Experimental Example 3: Evaluation of moisturizing and skin tone improving effect

(1) Test method

Twenty-one adult women aged 20 to 60 years were subjected to experiment. After morning and evening cleansing twice a day for 4 weeks on the face of the subject, the skin lotion, The same amount of serum and cream was spread evenly over the facial area along the skin texture.

During the period of the human body application test, in addition to the cosmetic product of the present invention, the use of various functional cosmetics including the exfoliating agent which may affect the test result was prohibited and the pack or massage was not performed.

(2) Testing site

The human skin test was carried out at the Korea Dermatological Institute with the same cleanser after the cleansing for 30 minutes in a constant temperature and humidity room (temperature: 22 ± 2 ° C, humidity: 50 ± 5%).

(3) Evaluation of moisturization improvement by Epsilon E100

In this test, Epsilon E100 (Biox Systems Ltd.) was applied to evaluate the moisturizing effect of the test materials. Epsilon E100 calculates the Epsilon (ε) value of the skin surface contacted with the sensor, and the higher the skin moisture content, the more the brightness of the image is increased and the blue part appears to be close to white. The same test person measured the area of the left eye of all subjects. The analysis of skin moisture was analyzed using Epsilon E100 Software Installer V2, an analysis program for Epsilon E100. This means that as the measured value increases compared to before the test substance is used, the moisturization is improved. Instrument measurements were taken prior to the use of the test substance, after 2 weeks of use, and after 4 weeks of use.

(4) Evaluation of improvement of skin tone by spectrophotometer and panoramic photographing system

In this test, spectrophotometer CM-2600D (Konica Minolta) and front-view imaging system (Robo Skin Analyzer CS50, Inforward, Inc.) were applied to evaluate the improvement of skin tone of test materials. The spectrophotometer was used for the analysis by measuring the left portion of all the subjects to be tested three times consecutively by the same test person and calculating an average value thereof. The L * value is the brightness, the a * value is the redness, and the b * value is the yellowness. The measured value of the spectrophotometer is composed of three factors L *, a * and b * . The L * value, which indicates skin brightness, was used to measure the improvement of the skin tone of the test substance. This means that the skin tone was improved as the measured value increased compared to before the test substance was used. The panoramic system measured all the subjects' facial areas by the same examiner in the same position as the uniform illumination for consistent imaging. In order to secure the hairline of the subject, the hair was fixed with a hair band, and the face of the face was photographed while the face was fixed to the top of the tool bar under the cage by wearing the same top gown and sitting on a chair. Instrument measurements were taken prior to the use of the test substance, after 2 weeks of use, and after 4 weeks of use.

(5) Survey

A questionnaire survey was conducted on the general skin condition characteristics of the subject, the skin condition before and after the use of the test substance, and the feeling of use of the test substance for the cosmetic composition of 21 adult women.

(6) Statistical analysis method

Statistical analysis of this study was performed using SPSS 17.0 for Windows program. The mean, standard deviation, frequency, and percentages were used to analyze the questionnaire of the subjects and a paired t-test analysis was conducted to analyze the change in the instrumental measurement results for various skin improvement degrees. Respectively.

<Result>

(1) Efficacy of Leaf and Cordyceps Extract

As shown in FIG. 4, the leaf extract has antioxidant functions by inhibiting DPPH-induced radical production in a concentration-dependent manner (FIG. 4A), but particularly when the leaves and the Cordyceps sinensis extract are treated simultaneously (FIG. 4B) Respectively. Leaf and Cordyceps sinensis extracts also promote type 1 procollagen gene expression in a concentration-dependent manner, as shown in Fig. In particular, even when treated with each of them, they showed an excellent effect of increasing type 1 collagen expression, but they exhibited a distinct wrinkle-improving synergistic effect when they were treated at the same time (FIG. 5C). Therefore, in the case of a cosmetic composition containing both a leaf extract and a Chinese cabbage extract, antioxidative and wrinkle-reducing effects are expected to be improved while reducing the amount of the extract.

In addition, as shown in Fig. 6, the leaves and Cordyceps sinensis extracts were promoted to be expressed upon photoaging by ultraviolet light, thereby reducing the expression of MMP gene promoting collagen degradation. In particular, the leaf extract reduces the expression of the MMP-1,2,9 gene, and the Cordyceps sinensis extract decreases the expression of the MMP-2,9 gene, which is expected to prevent collagen degradation and improve wrinkles and elasticity.

As shown in FIG. 6, the leaf and Cordyceps extract also decreased the expression of the inflammation-related COX-2 gene at a concentration of 100 μg / ml and 12.5 μg / ml, respectively, Lt; / RTI &gt; inhibited the formation of inflammatory factor NO &lt; RTI ID = 0.0 &gt;

7, the expression of HAS, TGM-1, and FLG genes associated with moisturizing was increased. Among them, the extracts of lysozyme increased the expression of HAS-3 gene involved in hyaluronic acid synthesis in a concentration-dependent manner And the extract of Cordyceps sinensis extract was expected to have an excellent moisturizing effect by increasing the expression of HAS-1 gene at a concentration of 50 μg / ml.

(2) Evaluation of skin irritation

No irritation was observed at 30 minutes, 24 hours, or 48 hours after the removal of the patches, cream, serum, and lotion using the lavender and Cordyceps sinensis extract of the present invention. The average skin response was 0.00 and judged to be unstimulated according to the criteria.

(3) Evaluation of skin moisturization improvement

As a result of analyzing the degree of skin moisturizing improvement of the lotion, serum and cream using the cosmetic composition of the present invention, it was found that (i) statistically significant (p <0.001) (Fig. 10A and Fig. 10B), (ii) in the case of the serum, the moisturization improvement rate was 47.65% after two weeks of use and 78.59% after four weeks of use (Figs. 12A and 12B) , (Iii) cream showed 13.19% after 2 weeks of use and 24.80% after 4 weeks of use (FIGS. 14A and 14B). Also, in the image showing the amount of skin moisture (FIG. 15), it was observed that as the water content of the skin increased, the brightness of the image increased and the deep blue changed to the light blue color as compared with before the skin lotion, serum and cream. As a result, it was determined that the cosmetic composition of the present invention could be used as a cosmetic for skin moisturizing.

(4) Evaluation of skin tone improvement

As a result of analyzing the improvement of the skin tone of the lotion, serum and cream using the cosmetic composition of the present invention, it was found that (i) the skin lotion was statistically significant (p <.05) (Fig. 12C and 12D), and (ii) in the case of serum, 0.52% after 2 weeks and 0.83% after 4 weeks of use, respectively (Fig. 14C and 14D), (iii) cream showed 0.29% after 2 times of use and 0.56% after 4 weeks of use (Figs. 16C and 16D). In addition, in the skin tone improvement test (Fig. 16), it was observed that the skin tone was brighter in all of the lotion, serum and cream than before use. As a result, it was judged that the cosmetic composition of the present invention could be used as a cosmetic for improving skin tone.

(5) Sensory evaluation

The inventive lotion, Serum  And cream Feeling

Before using the test material, 75% or more of the 21 subjects felt dry, and more than 85% of the subjects had dullness. The subjects were satisfied with 95% or more of wetting, moisturizing or moisturizing ability after using lotion pectin microcapsules of the present invention and the lotion pectin microcapsules of the present invention for 4 weeks, and their absorbency was more than 90%. However, in the case of serum, the absorbency decreased from 95% in the second week to 90% in the fourth week, which seems to result in a slight decrease in absorption as a result of the insufficient skin moisture being filled by the serum of the present invention. In addition, 90% or more of the brightening was satisfied, and 100% of the subjects were satisfied with the cosmetic of the present invention as a whole.

The inventive lotion, Serum  And skin condition after cream use

In the case of the cosmetic lotion of the present invention, 75% of the subjects to be tested after 6 weeks of use showed improvement in skin dryness and skin tone. In the case of the serum of the present invention, 95% And that the cream of the present invention also improved skin dryness and skin tone by 95% of subjects after 4 weeks of use.

From these results, it was found that users feel satisfied with the use feeling of lotion, serum and cream including skin and cordycepsin extract capsule pectin microcapsules of the present invention and skin condition after use.

10: first flow channel
20: second flow channel
30: Third flow channel
40: bottleneck structure
50: first reaction channel
60: second reaction channel

Claims (7)

A cosmetic composition for skin improvement comprising a herbal medicine extract-containing pectin microcapsule, wherein the herbal medicine extract pectin microcapsule
(a) preparing a herbal extract-containing nanoparticle solution by ultrasonication of a mixture of an extract of any one selected from the group consisting of lobster, Cordyceps, and a mixture thereof, and mineral oil;
(b) mixing the nanoparticle solution of step (a) with a pectin aqueous solution comprising pectin powder and polyoxyethylene sorbitan monooleate to produce a first mixture;
(c) mixing the first mixture of step (b) with a glycerin solution comprising ethanol, water, glycerin and CaCl ₂ to prepare pectin microcapsules; And
(d) separating and drying the pectin microcapsules prepared in step (c)
Lt; / RTI &gt;
Wherein steps (a) - (c) are performed using a fluid channel system,
A first flow channel (10) through which the fluid channel system supplies the nanoparticle solution; A second flow channel (20) disposed to surround the first flow channel and supplying a pectin aqueous solution; A third flow channel (30) disposed to surround the second flow channel and supplying a glycerine solution; A bottleneck structure (40) in which the first flow channel and the second flow channel meet; A first reaction channel 50 extending from the bottleneck structure 40; And a second reaction channel (60) connected to the first reaction channel (50) and the third flow channel (30)
Wherein the feed flow rate (Q _oil ) of the nanoparticle solution in the first flow channel is 0.1 to 1.0 μl / min, ii) the feed flow rate (Q _pec ) of the pectin aqueous solution in the second flow channel is 1 to 5 [mu] L / min; And iii) the feed flow rate ( _Qgly ) of the glycerin solution in the third flow channel is 10 to 50 ㎕ / min. The cosmetic composition according to any one of the above items . delete The herbal medicine extract of claim 1, wherein the herbal medicine extract is selected from the group consisting of water, methanol, ethanol, propanol, butanol, propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, Wherein the cosmetic composition is extracted with at least one solvent selected from the group consisting of hexane, and mixtures thereof. The cosmetic composition according to claim 1, wherein the herbal extract pectin microcapsule comprises a core comprising a nanoparticle of herbal medicine extract and an oil, and an outer shell containing pectin which surrounds the core. The cosmetic composition according to claim 1, wherein the herbal medicine extract pectin microcapsule is contained in an amount of 0.01 to 20% based on the weight of the total cosmetic composition. The cosmetic composition according to claim 1, wherein the skin-improving effect of the cosmetic composition is at least one selected from the group consisting of skin moisturizing, antioxidant and wrinkle-improving effects. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, nutritional cream, massage cream, eye cream, serum, cleansing cream, cleansing foam, cleansing water, pack, body lotion, body cream, Wherein the cosmetic composition is one selected from the group consisting of body essence, makeup base, foundation, and body cleanser.

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