CN106619150A - Natural sun-screening and moisture-preserving lipidosome freeze-dried powder modified by gamma-PGA as well as preparation and applications of natural sun-screening and moisture-preserving lipidosome freeze-dried powder - Google Patents
Natural sun-screening and moisture-preserving lipidosome freeze-dried powder modified by gamma-PGA as well as preparation and applications of natural sun-screening and moisture-preserving lipidosome freeze-dried powder Download PDFInfo
- Publication number
- CN106619150A CN106619150A CN201710090612.8A CN201710090612A CN106619150A CN 106619150 A CN106619150 A CN 106619150A CN 201710090612 A CN201710090612 A CN 201710090612A CN 106619150 A CN106619150 A CN 106619150A
- Authority
- CN
- China
- Prior art keywords
- freeze
- dried powder
- gamma
- lipidosome
- natural sun
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/41—Amines
- A61K8/416—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4993—Derivatives containing from 2 to 10 oxyalkylene groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
The invention discloses natural sun-screening and moisture-preserving lipidosome freeze-dried powder modified by gamma-PGA. The natural sun-screening and moisture-preserving lipidosome freeze-dried powder modified by gamma-PGA is prepared from the following components in percentage by weight: 2-5 % of rutin and pineapple leaf extract, 15-35 % of phospholipids, 5-10 % of cholesterol, 2-8 % of a positive charge control agent, 3-12 % of Tween-80, 0.5-2 % of VE, 0.5-2 % of VC, 1-14 % of beta-glucan, 1-3 % of gamma-polyglutamic acid, 20-60 % of a freeze-drying protective additive, smaller than or equal to 5% of water, and the sum of the weight percentages of the components is 100%. The preparation method of the natural sun-screening and moisture-preserving lipidosome freeze-dried powder comprises the following steps: encapsulating rutin and the pineapple leaf extract by adopting lipidosome, carrying out preparing to obtain DPPC thermosensitive lipidosome, and modifying the lipidosome by adopting gamma-polyglutamic acid. The hydrated lipidosome freeze-dried powder provided by the invention can be directly added into a cosmetic, can screen the sun and preserve moisture, is low in irritation, and is good in transdermal absorption effect, and the release rate of the active ingredients is high.
Description
Technical field
The invention belongs to cosmetic material preparing technical field, is related to a kind of day of γ-PGA (gamma-polyglutamic acid -) modification
Right sun-proof moisturizing lipidosome freeze-dried powder, the invention further relates to the preparation method and application of the liposome.
Background technology
Ozone layer it is continuous thinning, cause reach ground ultraviolet radiation (UVR) intensity enhancing, excessive exposure ultraviolet
(UV) the problems such as causing sunburn, pigmentation, solar dermatitis or even skin carcinoma.For this purpose, antitan agent has caused extensive pass
Note.
Sunscreen product on market is to reach broad spectrum ultraviolet light by adding physical sunscreen agent and chemical sun to prevent mostly
The effect of shield, but physical sunscreen agent has that protective capacities are weak, easy blocking, and then there is light stability in chemical sun
Poor, irritant the problems such as.There is in recent years the natural plant composition of sun-screening function, not only with excellent sun-proof performance, and
And meet demand of the people in terms of safety, environmental protection.
Scholar is had at present to screen natural sun-screening agents and developed, and is directly appended in cosmetics, but
It is affected by self stability etc., and Transdermal absorption is undesirable；Most sun care preparationss composition is single, does not reach broad absorption
The effect of ultraviolet；And the moistening effect of most sun care preparationss is not satisfactory.
The content of the invention
It is an object of the present invention to provide a kind of natural sun-prevention moisturizing lipidosome freeze-dried powder of gamma-polyglutamic acid-modification,
The UV absorbent of liposomal encapsulated two kinds of complementary types, solves that existing sunscreen product composition is single, stability is poor, function
Single problem.
It is a further object to provide the natural sun-prevention moisturizing lipidosome freeze-dried powder of above-mentioned gamma-polyglutamic acid-modification
Preparation method.
Third object of the present invention is to provide the natural sun-prevention moisturizing lipidosome freeze-dried powder of above-mentioned gamma-polyglutamic acid-modification
Application.
First technical scheme that the present invention is adopted be, a kind of natural sun-prevention moisturizing liposome of gamma-polyglutamic acid-modification
Lyophilized powder, by mass percentage including following components：Rutin and pineapple leaves extract 2～5%, phosphatidase 1 5～35%, cholesterol 5
～10%, positive charge control agent 2～8%, Tween-803～12%, VE 0.5～2%, VC 0.5～2%, beta glucan 1～
14%, gamma-polyglutamic acid -1～3%, freeze drying protectant 20～60%, water≤5%, the quality summation of above each component is
100%.
Preferably, phospholipid be dipalmitoyl phosphatidyl choline (DPPC) or with dimyristoyl phosphatidyl choline (DMPC),
A kind of mixture of hydrolecithin (HSPC), lysophosphatide (MSPC), DPPG (DPPG) etc.；Lyophilizing
Protective agent is trehalose and mannitol mixture；Positive charge control agent is 18-amine., PHOSPHATIDYL ETHANOLAMINE or dialkyl ether ammonium salt
In one or more；
Preferably, the mass ratio of rutin and pineapple leaves extract is 1:1～3:1.
Another technical scheme of the present invention is that the natural sun-prevention moisturizing of above-mentioned gamma-polyglutamic acid-modification is lipidosome freeze-dried
The preparation method of powder, following steps：
Step 1, by following mass percent raw material components are weighed：Rutin and pineapple leaves extract 2～5%, phosphatidase 1 5～
35%, cholesterol 5～10%, positive charge control agent 2～8%, T ween-803～12%, VE0.5～2%, VC 0.5～
2%, beta glucan 1～14%, gamma-polyglutamic acid -1～3%, freeze drying protectant 20～60%, the quality summation of above each component
It is 100%.
Step 2：Phospholipid, cholesterol, VE, Tween-80, positive charge control agent, rutin and pineapple leaves extract are dissolved in into nothing
Water-ethanol obtains A liquid, and A liquid rotary evaporation is removed into dehydrated alcohol, and forms one layer of lipid membrane on the wall.
Step 3：VC, beta glucan and freeze drying protectant are dissolved in into phosphate buffered solution (PBS), B liquid is obtained.
Step 4：In the lipid membrane for adding step 2 to obtain B solution, film separation is made, after abundant aquation, obtain liposome
Suspension C liquid.
Step 5：Gamma-polyglutamic acid-is dissolved in into water, and adds liposome turbid liquor C liquid, gamma-polyglutamic acid-is obtained after centrifugation
The natural sun-prevention moisturizing liposome of modification, then sequentially pass through obtain after film granulate, freeze thawing, lyophilization gamma-polyglutamic acid-modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Preferably, 30～40 DEG C of step 2 rotating evaporation temperature, 0.008～0.01Mpa of vacuum.
Preferably, gamma-polyglutamic acid-molecular weight is 70～1,000,000 in step 5, and gamma-polyglutamic acid-concentration of aqueous solution is
0.1%, pH are 5～6.
Preferably, step 5 film granulate excessively is：Sequentially pass through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μ
M filter membranes carry out granulate.
Preferably, step 5 freeze thawing treatment process is：30～60min is freezed first in -60 DEG C of environment, 15 are subsequently placed in
Melt it in～20 DEG C of environment, after the completion of thawing in 15～20 DEG C of environment supersound process 1min, circular treatment 3～4 times.
Preferably, step 5 freeze-drying process process is：Under -60 ± 1 DEG C of environmental condition after pre-freeze 10h, then -52
± 2 DEG C, vacuum less than 100mT environmental condition under lyophilization 25h.
Present invention also offers the natural sun-prevention moisturizing lipidosome freeze-dried powder of above-mentioned gamma-polyglutamic acid-modification is in cosmetics
Application.
When preparing sun care preparationss, deionized water aquation is added to the liposome of the encapsulating sun-prevention component of above-mentioned γ-PGA modifications
Then it is directly added in cosmetics afterwards, can be applicable to various State of cosmetics such as emulsion, sun-proof cream, spraying, facial film or water preparation.
Addition of the liposome of the encapsulating sun-prevention component of γ-PGA modifications in sun care preparationss is 0.2～15%.
The present invention is from rutin and pineapple leaves extract as UV absorbent, absorbing activity of the rutin to UVA, UVC
Greatly, pineapple leaves extract is big to the absorbing activity of UVB, UVC, because two kinds of active components compounding has to ultra-violet absorption activity
Complementarity, two kinds is used in mixed way the effect that can reach broad absorption ultraviolet.And directly addition is used, light in environment,
The factors such as metal ion and pH value in heat, oxygen and system, have a strong impact on its stability and percutaneous absorbability.
Active component or medicine are wrapped up and are prepared into nanometer liposome by the present invention using liposome technology, can be promoted
Skin absorbs.Liposome is the completely enclosed multilamellar vesicle of the bilayer formed by lipoids such as phospholipid, cholesterol.For making up
The liposome of product can encapsulate the material of stability difference, improve the zest of raw material, with targeting, slow release be controllable and safety non-toxic
The characteristics of, while the phospholipid bilayer of liposome may also help in during effective ingredient preferably penetrates to the skin, it is wide
It is general to be applied in cosmetics.
The present invention adds dipalmitoyl phosphatidyl choline (DPPC) in membrane material, and conventional liposome is prepared into into DPPC temperature
Quick property liposome, when ambient temperature reaches at or above phase transition temperature (Tm) of membrane material, phospholipid bilayer is tied by " gel " state
To " liquid crystal " state structural transformation, the acyl chain turbulence increase of hydrophobic side in phospholipid result in structure, and the mobility of liposome membrane increases
Greatly, the release speed of the medicine or active component that are encapsulated in lipid core and the medicine being embedded in inside bilayer or active component
Rate increases, performance its effect that can be bigger.
The present invention is also modified liposome using gamma-polyglutamic acid-.Gamma-polyglutamic acid-(γ-PGA) is that one kind is more
Polycarboxylic acids, with extremely strong moisture retention.Cosmetics containing γ-PGA, can form thin film on skin, prevent moisture from steaming
Send out, or even the self-regeneration function that skin can also be recovered.The effective ingredient that positive charge is taken in gamma-polyglutamic acid-and liposome occurs
Molecular action, causes effective ingredient slowly to discharge within the relatively long cycle and act on skin, beneficial to skin with its slow-release capability
Fully absorbing for skin, plays the effect of effective ingredient.
The invention has the beneficial effects as follows:
(1) natural sun-prevention moisturizing lipidosome freeze-dried powder of the invention encloses the UV absorbent of two kinds of complementary types, can
To reach the efficient absorption of ultraviolet.
(2) zest is low, and Transdermal absorption effect is good, solves directly to make an addition to and there is certain zest in cosmetics,
Stability is poor, the problems such as Transdermal absorption ability is not satisfactory；
(3) rate of release of the sun-proof moisturizing active component of the invention when temperature reaches to a certain degree is big, can be bigger send out
Wave its effect.
(4) moisture-keeping efficacy can also be played while effective protection skin sunburn of the present invention.
Description of the drawings
Fig. 1 is natural sun-prevention moisturizing lipidosome freeze-dried powder preparation method flow chart of the present invention；
Fig. 2 is invented liposomes lyophilized powder grain size distribution；
Fig. 3 is the liposome transdermal absorption figure of natural sun-prevention component of the present invention；
Fig. 4 is that the liposome Cumulative release amount of natural sun-prevention component of the present invention varies with temperature figure；
Fig. 5 is the liposome ultraviolet absorbance figure of natural sun-prevention component of the present invention.
Specific embodiment
With reference to the accompanying drawings and detailed description the present invention is described in further detail, but the present invention is not limited to
These embodiments.
Embodiment 1
Preparation method is as shown in Figure 1.
Step 1：Weigh dipalmitoyl phosphatidyl choline (DPPC) 2.40g respectively, cholesterol 0.80g, VE0.06g,
Tween-80 0.30g, 18-amine. 0.36g, rutin 0.12g, pineapple leaves extract 0.12g, are dissolved in 42mL dehydrated alcohol and obtain A liquid；
Step 2：35 DEG C on Rotary Evaporators, the evaporation of 0.009Mpa rotating pressure-decreasings is except anhydrous for the solution A that step 1 is obtained
Ethanol forms one layer of lipid membrane；
Step 3：Beta glucan 0.58g, VC 0.08g, trehalose 1.6g, Mannitol 1.6g are dissolved in into 129mL pH=7.0
Phosphate buffered solution obtain B liquid；Decompression rotary evaporation 50min is added to make film separation B liquid, after abundant aquation, 35 DEG C of water
Ultrasound 15min obtains liposome turbid liquor in bath.
Step 4：Compound concentration is 0.1% gamma-polyglutamic acid-aqueous solution, and it is 5 to adjust pH with NaOH, stirs 20min；
Step 5：The liposome solutions of the encapsulating natural sun-prevention component of step 3 are added to the polyglutamic acid solution of step 4, centrifugation
The natural sun-prevention moisturizing liposome of gamma-polyglutamic acid-modification is obtained afterwards；
Step 6：Sequentially passing through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane carries out granulate.
Step 7：60min is freezed in -60 DEG C of environment, being subsequently placed in 15 DEG C of environment melts it, after the completion of thawing
Supersound process 1min in 15 DEG C of environment, circular treatment 3 times, is distributed in cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then the lyophilization 25h under -52 ± 2 DEG C, environmental condition of the vacuum less than 100mT, obtain gamma-polyglutamic acid-modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of gamma-polyglutamic acid-modification obtained in the present embodiment
Than for rutin and pineapple leaves extract 2.81%, phosphatidase 2 8.10%, cholesterol 9.37%, positive charge control agent 4.21%,
Tween-80 3.51%, VE 0.70%, VC 0.94%, beta glucan 6.79%, gamma-polyglutamic acid -2.00%, frozen-dried protective
Agent 37.47%, water 4.10%.
Embodiment 2
Step 1：Weigh dipalmitoyl phosphatidyl choline (DPPC) and DPPG (DPPG) 4.90g
(DPPC:DPPG=10:1), cholesterol 1.30g, VE 0.10g, Tween-80 0.40g, two-octadecane-two-methyl amine salt
0.60g, rutin 0.30g, pineapple leaves extract 0.20g, are dissolved in 78mL dehydrated alcohol and obtain A liquid；
Step 2：40 DEG C on Rotary Evaporators, the evaporation of 0.01Mpa rotating pressure-decreasings is except anhydrous for the solution A that step 1 is obtained
Ethanol forms one layer of lipid membrane；
Step 3：Beta glucan 0.50g, VC 0.10g, trehalose 3.2g, Mannitol 3.2g are dissolved in into 233mL pH=6.9
Phosphate buffered solution obtain B liquid；Decompression rotary evaporation 50min is added to make film separation B liquid, after abundant aquation, 35 DEG C of water
Ultrasound 15min obtains liposome turbid liquor in bath.
Step 4：Compound concentration is 0.1% gamma-polyglutamic acid-aqueous solution, and it is 5.5 to adjust pH with NaOH, stirs 20min；
Step 5：The liposome solutions of the encapsulating natural sun-prevention component of step 3 are added to the gamma-polyglutamic acid-solution of step 4,
The natural sun-prevention moisturizing liposome of gamma-polyglutamic acid-modification is obtained after centrifugation；
Step 6：Sequentially passing through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane carries out granulate.
Step 7：60min is freezed in -60 DEG C of environment, being subsequently placed in 18 DEG C of environment melts it, after the completion of thawing
Supersound process 1min in 18 DEG C of environment, circular treatment 3 times, is distributed in cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then the lyophilization 25h under -52 ± 2 DEG C, environmental condition of the vacuum less than 100mT, obtain gamma-polyglutamic acid-modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of gamma-polyglutamic acid-modification obtained in the present embodiment
Than for rutin and pineapple leaves extract 3.24%, phosphatidase 3 2.42%, cholesterol 8.43%, positive charge control agent 3.87%,
Tween-80 2.59%, VE 0.65%, VC 0.65%, beta glucan 3.24%, gamma-polyglutamic acid -2.02%, frozen-dried protective
Agent 41.50%, water 1.36%.
Embodiment 3
Step 1：Weigh dipalmitoyl phosphatidyl choline (DPPC) and dimyristoyl phosphatidyl choline (DMPC) 1.60g
(DPPC:DMPC=10:1), cholesterol 0.46g, VE 0.18g, Tween-80 1.00g, DOPE
(DOPE) 0.60g, rutin 0.12g, pineapple leaves extract 0.04g, are dissolved in 40mL dehydrated alcohol and obtain A liquid；
Step 2：30 DEG C on Rotary Evaporators, the evaporation of 0.008Mpa rotating pressure-decreasings is except anhydrous for the solution A that step 1 is obtained
Ethanol forms one layer of lipid membrane；
Step 3：Beta glucan 1.10g, VC 0.18g, trehalose 1.6g, Mannitol 1.6g are dissolved in into 150mL pH=6.8
Phosphate buffered solution obtain B liquid；Decompression rotary evaporation 50min is added to make film separation B liquid, after abundant aquation, 35 DEG C of water
Ultrasound 15min obtains liposome turbid liquor in bath；
Step 4：Compound concentration is 0.1% gamma-polyglutamic acid-aqueous solution, and it is 6 to adjust pH with NaOH, stirs 20min；
Step 5：The liposome of the encapsulating natural sun-prevention component of step 3 is added to the polyglutamic acid solution of step 4, after centrifugation
To the natural sun-prevention moisturizing liposome of gamma-polyglutamic acid-modification；
Step 6：Sequentially passing through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter membrane carries out granulate.
Step 7：60min is freezed in -60 DEG C of environment, being subsequently placed in 20 DEG C of environment melts it, after the completion of thawing
Supersound process 1min in 20 DEG C of environment, circular treatment 3 times, is distributed in cillin bottle the pre-freeze under -60 ± 1 DEG C of environmental condition
After 10h, then the lyophilization 25h under -52 ± 2 DEG C, environmental condition of the vacuum less than 100mT, obtain gamma-polyglutamic acid-modification
Natural sun-prevention moisturizing lipidosome freeze-dried powder.
Its constituent mass percentage of the natural sun-prevention moisturizing lipidosome freeze-dried powder of gamma-polyglutamic acid-modification obtained in the present embodiment
Than for rutin and pineapple leaves extract 1.79%, phosphatidase 1 7.94%, cholesterol 1.79%, positive charge control agent 6.73%,
Tween-80 11.21%, VE 2.02%, VC 2.02%, beta glucan 12.33%, gamma-polyglutamic acid -2.13%, lyophilizing is protected
Shield agent 35.87%, water 2.80%.
After testing, the mean diameter of invented liposomes suspension is 280 ± 30nm, as shown in Figure 2.
Investigate the present invention prepare gamma-polyglutamic acid-modification natural sun-prevention moisturizing liposome effect, with embodiment 1,
As a example by the lipidosome freeze-dried powder of embodiment 2 and embodiment 3, the detection of following aspect is carried out：
1. temperature sensitivity detection
Method of testing is：Lipidosome freeze-dried powder in Example 1 is dissolved in right amount after water and takes 10mL and be placed in bag filter, puts
Discharge in normal saline, 35 DEG C to 44 DEG C of temperature range discharges 20min, a random sample is taken respectively in the case of each design temperature
Determined with HPLC and detected, obtain medicine Cumulative release amount.As shown in figure 3, it can be seen that active in 35 DEG C to 39 DEG C
The release of composition has almost no change, the burst size increase in the range of 39 DEG C to 42 DEG C when temperature, as temperature continues to raise, releases
High-volume tend to be steady.Quick release of the active component at 39 DEG C is attributed to first 38.13 DEG C of phase transition temperature, when temperature rises
Tend to be steady by its second phase transition temperature 41.99 DEG C to burst size when 42 DEG C and determine, when the burst size in the range of 35 DEG C to 39 DEG C
Only slight increase is varied with temperature, is release temperature the reason for there is such case less than temperature sensitive phase change temperature of liposome, phosphorus
The permeance property of lipid bilayer only slightly changes.
2. percutaneous abilities detection
Sample sets be the lipidosome freeze-dried powder of embodiment 1, reference substance be prepare concentration same as Example 1 sun-prevention component it is molten
Liquid, difference is sun-prevention component in reference substance without liposome.
Method of testing is：Tested using isolated skin Transdermal absorption, in vitro rabbit in uniform thickness is chosen, with normal saline
Rinse well, freezen protective.Using Franz transdermal diffusion apparatus, rabbit is placed in into up and down between room of Franz diffusion cells, cutin
Layer upward, takes test sample and is applied to skin surface, and phosphate buffered solution, built-in stirrer, keeping temperature 36 are contained in reception tank
±1℃.Whole reception liquids are taken out respectively at 0.5,2,4,6,8,10,12,24h, and supplements equal-volume reception liquid rapidly.Using height
Effect liquid phase chromatogram instrument is detected, and calculates Percutaneous permeability Q (the μ g/cm of sun-prevention component2)。
Test concrete outcome is shown in Fig. 4, as can be seen from the figure the natural sun-prevention moisturizing liposome after gamma-polyglutamic acid-modification
Each sample point Transdermal absorption effect apparently higher than the sun-prevention component without liposome transdermal effect, illustrate γ-
Liposome after polyglutamic acid modification can improve the problem that transdermal is obstructed.
3. sun-proof function test
A, sun-proof spf value are determined
Test object is two comparative examples of three embodiments of the invention and setting, two comparative example preparation processes and bar
Part is same as Example 3, and difference is that rutin 0.16g is added in the step of comparative example 1 (1), is not added with pineapple leaves extract,
Step (1) adds pineapple leaves extract 0.16g in comparative example 2, is not added with rutin.
Using the portable spectrometer Optometrics LLC SPF-290S of u s company Optometrics productions in room
Inside measure, in order to ensure certainty of measurement during measurement, its operating process is carried out in strict accordance with the measuring process that the instrument specifies：
Test environment conditions：Thermostatic constant wet chamber, 22 DEG C ± 1 DEG C of temperature, humidity 50% ± 10%.
Appropriate standard specimen or sample (2mg/cm are drawn with one-shot injector2), it is uniformly put on PMMA plates, put on latex
Finger-cover for medical use is smeared uniformly, and in dark 30min is placed.After preheater apparatus 15min, blank plate is inserted, adjust light intensity and maximum ripple
Grow to by instrument preliminary examination, be subsequently inserted into standard specimen and sample panel, randomly select 8 points of tests, draw MPF curves, it is supporting by instrument
Computed in software draws sample spf value.Test concrete outcome is shown in Table 2.
The measure of the sample spf value of table 2
From table 2 it can be seen that the spf value of the natural sun-prevention liposome of the gamma-polyglutamic acid-modification of present invention preparation is more single
The height of one sun-prevention component, effect of its combination is obviously improved, with certain protection effect.
B, ultra-violet absorption determination of activity
Liposome accurate weighing 100mg obtained by embodiment 3 and comparative example 1, comparative example 2 is dissolved in into 50mL volumetric flasks
In, addition dehydrated alcohol makes solution as solvent.Will made by solution down in the quartz cuvette pond of 1cm × 1cm × 5cm,
The wave-length coverage ultraviolet absorbance of 200～400nm is measured in the ultra-violet and visible spectrophotometer debugged.Test result
As shown in figure 5, it can be seen that the only encapsulating pineapple leaves extract liposome for preparing is prepared in UVA mean light absorbencies ratio
Only the liposome of encapsulating rutin is low in UVA mean absorbance values, and in UVB, UVC interval mean absorbance values than only encapsulating
The liposome of rutin is high, and preparation is ultraviolet in absorption as the liposome of UV absorbent using rutin and pineapple leaves extract
Line effect is compared with single component more preferably.
Claims (10)
1. the natural sun-prevention moisturizing lipidosome freeze-dried powder of a kind of γ-PGA modification, it is characterised in that include by mass percentage with
Lower component：Rutin and pineapple leaves extract 2～5%, phosphatidase 1 5～35%, cholesterol 5～10%, positive charge control agent 2～
8%, Tween-803～12%, VE 0.5～2%, VC 0.5～2%, beta glucan 1～14%, gamma-polyglutamic acid -1～
3%, freeze drying protectant 20～60%, water≤5%, the quality summation of above each component is 100%.
2. the natural sun-prevention moisturizing lipidosome freeze-dried powder that γ-PGA according to claim 1 are modified, it is characterised in that phospholipid
For dipalmitoyl phosphatidyl choline, or for dimyristoyl phosphatidyl choline, hydrolecithin, lysophosphatide, two palmityl phosphorus
Any one mixture with dipalmitoyl phosphatidyl choline in phosphatidyl glycerol；Freeze drying protectant is that trehalose and Mannitol mix
Thing；Positive charge control agent is one or more in 18-amine., PHOSPHATIDYL ETHANOLAMINE or dialkyl ether ammonium salt.
3. the natural sun-prevention moisturizing lipidosome freeze-dried powder that γ-PGA according to claim 1 are modified, it is characterised in that rutin
It is 1 with the mass ratio of pineapple leaves extract:1～3:1.
4. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA as claimed in claim 1 modification, its feature exists
In following steps：
Step 1, by following mass percent raw material components are weighed：Rutin and pineapple leaves extract 2～5%, phosphatidase 1 5～35%,
Cholesterol 5～10%, positive charge control agent 2～8%, Tween-803～12%, VE0.5～2%, VC 0.5～2%, β-Portugal
Polysaccharide 1～14%, gamma-polyglutamic acid -1～3%, freeze drying protectant 20～60%, the quality summation of above each component is 100%；
Step 2：Phospholipid, cholesterol, VE, Tween-80, positive charge control agent, rutin and pineapple leaves extract are dissolved in into anhydrous second
Alcohol obtains A liquid, and the evaporation of A liquid is removed into dehydrated alcohol, and forms one layer of lipid membrane on the wall；
Step 3：VC, beta glucan and freeze drying protectant are dissolved in into phosphate buffered solution (PBS), B liquid is obtained；
Step 4：In the lipid membrane for adding step 2 to obtain B solution, film separation is made, after abundant aquation, obtain liposome suspension
Liquid C liquid；
Step 5：Gamma-polyglutamic acid-is dissolved in into water, and adds liposome turbid liquor C liquid, gamma-polyglutamic acid-modification is obtained after centrifugation
Natural sun-prevention moisturizing liposome, then sequentially pass through obtain after film granulate, freeze thawing, lyophilization gamma-polyglutamic acid-modification day
Right sun-proof moisturizing lipidosome freeze-dried powder.
5. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA according to claim 4 modification, it is special
Levy and be, 30～40 DEG C of rotating evaporation temperature described in step 2,0.008～0.01Mpa of vacuum.
6. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA according to claim 4 modification, it is special
Levy and be, gamma-polyglutamic acid-molecular weight described in step 5 is 70～1,000,000, gamma-polyglutamic acid-concentration of aqueous solution is 0.1%, pH
For 5～6.
7. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA according to claim 4 modification, it is special
Levy and be, film granulate is crossed described in step 5 is：Sequentially pass through 0.8 μm of filter membrane, 0.65 μm of filter membrane, 0.45 μm of filter membrane and 0.22 μm of filter
Film carries out granulate.
8. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA according to claim 4 modification, it is special
Levy and be, freeze thawing treatment process is described in step 5：30～60min is freezed first in -60 DEG C of environment, 15～20 are subsequently placed in
Melt it in DEG C environment, after the completion of thawing in 15～20 DEG C of environment supersound process 1min, circular treatment 3～4 times.
9. the preparation method of the natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA according to claim 4 modification, it is special
Levy and be, freeze-drying process process is described in step 5：Under -60 ± 1 DEG C of environmental condition after pre-freeze 10h, then -52 ± 2
DEG C, vacuum less than 100mT environmental condition under lyophilization 25h.
10. the natural sun-prevention moisturizing lipidosome freeze-dried powder that the γ-PGA as any one of claim 1-3 are modified is in cosmetics
In application, it is characterised in that by the γ-PGA modify natural sun-prevention moisturizing lipidosome freeze-dried powder add after water hydratable then
In being directly added into cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710090612.8A CN106619150B (en) | 2017-02-20 | 2017-02-20 | The natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification and its preparation and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710090612.8A CN106619150B (en) | 2017-02-20 | 2017-02-20 | The natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification and its preparation and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106619150A true CN106619150A (en) | 2017-05-10 |
| CN106619150B CN106619150B (en) | 2019-05-31 |
Family
ID=58845574
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710090612.8A Active CN106619150B (en) | 2017-02-20 | 2017-02-20 | The natural sun-prevention moisturizing lipidosome freeze-dried powder of γ-PGA modification and its preparation and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106619150B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107822979A (en) * | 2017-11-30 | 2018-03-23 | 宁波秋日小城智能科技有限公司 | A kind of preparation method of the freeze-dried powder of the composition with acne-removing |
| CN107875059A (en) * | 2017-11-30 | 2018-04-06 | 宁波秋日小城智能科技有限公司 | A kind of composition with acne-removing |
| CN108158852A (en) * | 2017-12-29 | 2018-06-15 | 青岛百瑞吉生物工程有限公司 | A kind of sunscreen spray and preparation method thereof |
| CN112656696A (en) * | 2020-12-23 | 2021-04-16 | 杭州优玛达生物科技有限公司 | Composition of molecular motor lipid vesicles and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940040A (en) * | 2015-07-09 | 2015-09-30 | 西安艾尔菲生物科技有限公司 | Liposomes modified with hyaluronic acid and used for encapsulating whitening components, preparation method and application |
| CN104983591A (en) * | 2015-07-09 | 2015-10-21 | 西安艾尔菲生物科技有限公司 | Double modified beta-carotene lipidosome and preparation method thereof |
| CN106176563A (en) * | 2016-07-26 | 2016-12-07 | 西安艾尔菲生物科技有限公司 | People's umbilical cord lipidosome freeze-dried powder of MSC serum-free medium and preparation thereof and application |
-
2017
- 2017-02-20 CN CN201710090612.8A patent/CN106619150B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940040A (en) * | 2015-07-09 | 2015-09-30 | 西安艾尔菲生物科技有限公司 | Liposomes modified with hyaluronic acid and used for encapsulating whitening components, preparation method and application |
| CN104983591A (en) * | 2015-07-09 | 2015-10-21 | 西安艾尔菲生物科技有限公司 | Double modified beta-carotene lipidosome and preparation method thereof |
| CN106176563A (en) * | 2016-07-26 | 2016-12-07 | 西安艾尔菲生物科技有限公司 | People's umbilical cord lipidosome freeze-dried powder of MSC serum-free medium and preparation thereof and application |
Non-Patent Citations (3)
| Title |
|---|
| 王其仁: "芦丁防晒霜的试制", 《中国实用医药》 * |
| 郭飞燕: "菠萝叶中防晒成分的超声提取及其稳定性研究", 《化学研究与应用》 * |
| 陈代杰: "《生物产业》", 30 June 2014, 上海：上海科学技术文献出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107822979A (en) * | 2017-11-30 | 2018-03-23 | 宁波秋日小城智能科技有限公司 | A kind of preparation method of the freeze-dried powder of the composition with acne-removing |
| CN107875059A (en) * | 2017-11-30 | 2018-04-06 | 宁波秋日小城智能科技有限公司 | A kind of composition with acne-removing |
| CN107822979B (en) * | 2017-11-30 | 2020-11-10 | 广州领衔生物科技有限公司 | Preparation method of freeze-dried powder of composition with acne removing effect |
| CN108158852A (en) * | 2017-12-29 | 2018-06-15 | 青岛百瑞吉生物工程有限公司 | A kind of sunscreen spray and preparation method thereof |
| CN112656696A (en) * | 2020-12-23 | 2021-04-16 | 杭州优玛达生物科技有限公司 | Composition of molecular motor lipid vesicles and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106619150B (en) | 2019-05-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104983591B (en) | A kind of dual modified beta-carotenoid lipid body and preparation method thereof | |
| CN106619150A (en) | Natural sun-screening and moisture-preserving lipidosome freeze-dried powder modified by gamma-PGA as well as preparation and applications of natural sun-screening and moisture-preserving lipidosome freeze-dried powder | |
| Balakrishnan et al. | Formulation and in vitro assessment of minoxidil niosomes for enhanced skin delivery | |
| CN104997652B (en) | A kind of crease-resistant moisturizing liposome and its preparation method and application | |
| David et al. | Formulation and in vitro evaluation of ethosomes as vesicular carrier for enhanced topical delivery of isotretinoin | |
| KR100799991B1 (en) | Cosmetic composition for wrinkle care | |
| KR20130119587A (en) | Solubilization method of poor/non water-soluble ingredients through preparation method of multi lamellar liquid crystalline | |
| Zhang et al. | Sucrose esters improve the colloidal stability of nanoethosomal suspensions of (−)‐epigallocatechin gallate for enhancing the effectiveness against UVB‐induced skin damage | |
| Abosabaa et al. | Hybrid chitosan-lipid nanoparticles of green tea extract as natural anti-cellulite agent with superior in vivo potency: full synthesis and analysis | |
| CN114652636A (en) | Anti-wrinkle repair composition, preparation method thereof and cosmetic containing composition | |
| KR102551369B1 (en) | Transparent liposome composition containing centella asiatica extract | |
| Chen et al. | Premna microphylla Turcz pectin protected UVB-induced skin aging in BALB/c-nu mice via Nrf2 pathway | |
| US9498412B2 (en) | Method for producing microcapsules with a sun protection effect | |
| CN114099438A (en) | Kaempferol liposome gel and preparation method thereof | |
| CN108309954A (en) | A kind of the lecithin aliphatic radical reversed hexagonal liquid crystal medicament and preparation method of tea polyphenols | |
| CN103432020A (en) | Sustained-release sunscreen composition and preparation method and application thereof | |
| CN115414302A (en) | Skin-moistening and nourishing type moisturizing emulsion and preparation method thereof | |
| CN111603396B (en) | Poly natural polyphenol type sunscreen gel and preparation method thereof | |
| CN115282106A (en) | Sunscreen emulsion containing fructus Phyllanthi extract and its preparation method | |
| Jiang et al. | The dexamethasone acetate cubosomes as a potential transdermal delivery system for treating skin inflammation | |
| Ermawati et al. | The effect of propylene glycol and polyethylene glycol 400 on physicochemical properties of peel off mask of nanosilver using bioreductor Sweet Orange Peels (Citrus sinensis L. Osbeck) | |
| Hartini et al. | Formulation and Evaluation of Liposome Moringa Oleifera Seed Oil (Moringa oleifera L.) as Anti-aging | |
| Motwani et al. | Nano-Transfersomes of Vitamin-E and Aloe-Vera for The Management of Psoriasis: Nano-Transfersomes for the Management of Psoriasis | |
| CN113440463B (en) | Water-resistant protein-based sunscreen cream | |
| Chaurasiya et al. | Development and Characterization of Elastic Liposomes of Metronidazole for the Treatment of Bacterial Infection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |