CN103191232B - Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition - Google Patents
Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition Download PDFInfo
- Publication number
- CN103191232B CN103191232B CN201310124826.4A CN201310124826A CN103191232B CN 103191232 B CN103191232 B CN 103191232B CN 201310124826 A CN201310124826 A CN 201310124826A CN 103191232 B CN103191232 B CN 103191232B
- Authority
- CN
- China
- Prior art keywords
- preparation
- chinese medicine
- rhizoma coptidis
- weight
- fructus gardeniae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle. The traditional Chinese medicine composition is composed of coptis root, jasmine and gallnut in a weight ratio of 2: (2-4): (2-1), and the formulation is unique and satisfies the drug matching principle. Through the matching of the coptis root, jasmine and gallnut, the occlusion of papillary foramina can be strengthened to prevent pathogenic bacteria from passing the papillary foramina by virtue of the effects of clearing away heat and toxic materials and contracting smooth muscle of the jasmine. The invention further discloses a preparation containing the traditional Chinese medicine composition. The coptis root, jasmine fluid extract and gallnut fluid extract are combined, and then normal auxiliary materials are added to the combination to prepare the traditional Chinese medicine ointment. The preparation is a pure traditional Chinese medicine preparation with high security, is free of residues, and can be used in a lactation period; the preparation is applied to the papillary foramina so as to prevent the pathogenic bacteria from entering glandcistern from the papillary foramina; and meanwhile, the pathogenic bacteria in the papillary foramina and the glandcistern can be killed so as to effectively prevent mastitis of dairy cattle.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to Chinese medicine composition and the preparation thereof of preventing and treating mammitis of cow, also relate to the application of this Chinese medicine composition.
Background technology
Mastitis, milk is swollen, milk is yellow, acute mastitis, is that expectorant, wet, gas, blood stagnation knot are loose, changes and is due to inflammation.It is reported, milch cow has squeezed after milk, and teat sphincter is shunk need reach 1 hour completely, makes papillary foramina for a long time in open state, provides convenience for pathogenic microorganism enters Ru Chi, is therefore considered to the one of the main reasons of mammitis of cow morbidity.In practice, once there are a lot of raisers to prevent and treat in papillary foramina administration with antimicrobial ointment season occurred frequently at mammitis of cow, but owing to being to use the non-dry milk phase, will certainly cause antibiotic residual.Along with the raising of the attention degree of people to food safety, antibiotic is used for preventing and treating mammitis of cow and is also subject to certain restrictions.
Chinese medicine more and more receives people's concern at control mammitis of cow, be also the focus and emphasis of a lot of scholar's research, has injection, injectable powder, liniment, unguentum etc. at present in the dosage form of control mastitis.But under blood breast barrier or skin barrier effect, be difficult to reach active drug concentration in newborn pond, therapeutic effect is often undesirable.In order to make medicine reach valid density at Ru Chizhong, in prior art, also have drug infusion in newborn pond, but it is very difficult to adopt its operation of administration in this way, needs professional person's professional tool.The people such as Wang Jiufeng form thin film by " mastitis nipple Medicatedbath lotion " at body surface, but thereby reach the effect of killing papillary foramina pathogen, but milch cow is having squeezed the cattle farm that can couch unavoidably in 1 hour after milk, will certainly cause breaking of thin film, in addition a large amount of pathogenic microorganisms in cattle bed, can enter again completely not closed papillary foramina and cause morbidity.In addition, clinical investigation finds that nipple Medicatedbath lotion exists the shortcomings such as use is inconvenient, duration of efficacy is short.Therefore, a kind of control of exploitation mammitis of cow medicine, can effectively stop pathogen to pass through papillary foramina, but and the pathogen that can kill in papillary foramina be the important channel of control mammitis of cow.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of Chinese medicine composition of preventing and treating mammitis of cow, formula is unique, meets drug matching principle, good to the prevention effect of mastitis.
For achieving the above object, the invention provides following technical scheme:
The Chinese medicine composition of control mammitis of cow, described Chinese medicine composition by Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis by weight forming for 2:2-4:2-1.
Preferably, described Chinese medicine composition by Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis by weight forming for 2:2:1.
Two of object of the present invention is to provide the preparation of control mammitis of cow, and its technical scheme is:
2. contain the preparation of described Chinese medicine composition.
In order to facilitate administration, most preferred dosage form is ointment, the ointment making can adopt nipple administration, both (pathogen enters the main path in newborn pond can to have stoped pathogen to pass through open papillary foramina, because milch cow has squeezed milk papillary foramina president time open), but the cause of disease bacterium in papillary foramina and newborn pond can be killed again.
Preferably, described ointment comprises that percentage by weight is Rhizoma Coptidis, Fructus Gardeniae and the Galla Chinensis fluid extract of 10-15%.
Preferably, described ointment comprises that percentage by weight is 13% Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis fluid extract.
Preferred, the preparation method of Rhizoma Coptidis and Fructus Gardeniae fluid extract is: get Rhizoma Coptidis and the Fructus Gardeniae of formula ratio, and the ethanol extraction that is 75% by volume fraction, extracting solution is concentrated into medical material gross weight, obtains alcohol extract; Medicinal residues extracting in water, extracting solution is concentrated into medical material gross weight, obtains water extraction liquid, then merges alcohol extract and water extraction liquid, concentrated, obtains Rhizoma Coptidis and Fructus Gardeniae fluid extract.
Preferred, the preparation method of Rhizoma Coptidis and Fructus Gardeniae fluid extract is: get formula ratio Rhizoma Coptidis and Fructus Gardeniae, add and be equivalent to the soak with ethanol 30 minutes that the volume fraction of 5 times of Rhizoma Coptidis and Fructus Gardeniae weight is 75%, then be reflux, extract, 2 times under 80 DEG C of conditions in temperature, each 1 hour, filtrate is concentrated into medical material gross weight, obtains alcohol extract; In filtering residue, add the water that is equivalent to 8 times of filtering residue weight, heating extraction 3 times is extracted 1 hour at every turn, collect filtrate, and filtrate being concentrated into is equivalent to medical material gross weight, obtain water extraction liquid, then merge alcohol extract and water extraction liquid, and be concentrated into and be equivalent to medical material gross weight, obtain Rhizoma Coptidis and Fructus Gardeniae fluid extract.
Preferably, the preparation method of Galla Chinensis fluid extract: get the Galla Chinensis of formula ratio, add the water that is equivalent to 15 times of Galla Chinensis weight, be to extract 2 times under 100 DEG C of conditions in temperature, each 2 hours, then filtration collection filtrate be concentrated into and be equivalent to Galla Chinensis weight, obtains Galla Chinensis fluid extract.
Three of object of the present invention is to provide the application of above-mentioned Chinese medicine composition, and technical scheme is:
The application of described Chinese medicine composition in the medicine of preparation control mammitis of cow.
Beneficial effect of the present invention is: the Chinese medicine composition of control mammitis of cow disclosed by the invention, and formula is unique, has added Fructus Gardeniae in formula, there is heat-clearing and toxic substances removing, shrink the effect of smooth muscle, can strengthen the closure of papillary foramina simultaneously, stop pathogen to enter papillary foramina; The invention also discloses Chinese medicine composition is made to fluid extract, then add conventional adjuvant and make preparation (as ointment), can adopt nipple administration, overcome employing intramuscular injection or Bolos intravenous administration and can not effectively see through blood breast barrier, transdermal administration can not effectively pass through skin barrier, and can not make medicine reach the defect of valid density at Ru Chizhong, also overcome breast perfusion preparation needs the operational difficulties such as professional person's professional tool simultaneously; And use Chinese medicine ointment of the present invention, can make the effective ingredient in medicine enter in newborn pond and reach active drug concentration, to preventing and treating the effective of mammitis of cow, owing to adopting ointment, have easy to usely, only need to use several grams at every turn, and be convenient to remove, need be only to extrude to discard milking, pollution-free to milk, can meet the needs of food safety.
Detailed description of the invention
By specific embodiment, be described in detail of the present invention below.
Embodiment 1
The Chinese medicine composition of control mammitis of cow, its prescription is as follows:
Prescription 1: Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis weight ratio are 2:2:1.
Prescription 2: Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis weight ratio are 2:2:2.
Prescription 2: Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis weight ratio are 2:4:1.
Embodiment 2
The preparation of Chinese medicine fluid extract
1. preparation Rhizoma Coptidis, Fructus Gardeniae fluid extract:
Get respectively Rhizoma Coptidis and the Fructus Gardeniae of formula ratio, add and be equivalent to the soak with ethanol 30 minutes that the volume fraction of 5 times of Rhizoma Coptidis and Fructus Gardeniae weight is 75%, then be reflux, extract, 2 times under 80 DEG C of conditions in temperature, each 1 hour, filter and collect respectively filtrate and filtering residue, filtrate being concentrated into of collection is equivalent to medical material gross weight, is designated as filtrate A; The water that is equivalent to 8 times of filtering residue weight will be added in the filtering residue of collection, heating extraction 3 times, each extraction 1 hour, filter and collect filtrate, and filtrate being concentrated into is equivalent to medical material gross weight, be designated as liquor B, then merging filtrate A and liquor B, and be concentrated into and be equivalent to medical material gross weight, obtain Rhizoma Coptidis, Fructus Gardeniae fluid extract.
2. prepare Galla Chinensis fluid extract
Getting the Galla Chinensis of formula ratio, add the water that is equivalent to 15 times of Galla Chinensis weight, is to extract 2 times under 100 DEG C of conditions in temperature, and each 2 hours, then filtration collection filtrate be concentrated into and be equivalent to Galla Chinensis weight, obtains Galla Chinensis fluid extract.
The Rhizoma Coptidis of preparation, Fructus Gardeniae fluid extract and Galla Chinensis fluid extract are mixed, obtain Chinese medicine fluid extract.In gained Chinese medicine fluid extract, in every 1mL Chinese medicine fluid extract, be equivalent to 1g crude drug.
Embodiment 3
The Chinese medicine ointment of control mammitis of cow, its preparation method can adopt routine to prepare the method for ointment, and method for optimizing is:
Get glycerol, triethanolamine, distilled water is as water, then add benzoic acid, nipalgin, during sodium sulfite and EDETATE SODIUM are soluble in the aqueous phase, then get stearic acid, lanoline, liquid paraffin, white vaseline in mass ratio for 4:2.5:1.5:1 mix after as oil phase, then oil phase is mixed with water and heating in water bath to 85 DEG C carries out melting, add again sodium lauryl sulphate, tween 80 and octadecanol are as emulsifying agent, speed with 80 ~ 100r/min stirs by same direction, then the Chinese medicine fluid extract of getting formula ratio add with substrate mixed liquor in, mix homogeneously, condensation, must prevent and treat the Chinese medicine ointment of mammitis of cow.
In the Chinese medicine ointment of the control mammitis of cow making, the percentage by weight of Chinese medicine fluid extract is 15%, the percentage by weight of oil phase is 20%, the percentage by weight of emulsifying agent is 5%, glycerol percentage by weight is 5%, the percentage by weight of triethanolamine is 1.489%, benzoic percentage by weight is 0.25%, the percentage by weight of nipalgin antiseptic is 0.1%, the percentage by weight of sodium sulfite is 0.5%, the percentage by weight of EDETATE SODIUM is 0.01%, surplus is water, and wherein the HLB value of emulsifying agent is 13.
Further, the percentage by weight of Chinese medicine fluid extract is within the scope of 10-15%, and emulsifying agent all can be made the Chinese medicine ointment of control mammitis of cow within the scope of 3-5%.
Embodiment 4
Separating 4 strain mastitis pathogen (being respectively staphylococcus aureus, F group streptococcus, B streptococcus and D streptococcus) and 2 strain standard bacterium (for streptococcus (1886) and streptococcus (588)) from cattle farm, Rongchang County is strain subject, Chinese medicine fluid extract disclosed by the invention and 15 kinds of Chinese medicines are made to extracting solution and carry out extracorporeal bacteria inhibitor test, result is as shown in table 1.
Table 1, the 15 kinds of Chinese medicine extraction liquid MIC value (mg/mL) to main pathogen of bovine mastitis
| Chinese medicine | Staphylococcus aureus | B streptococcus | F streptococcus | D streptococcus | Streptococcus type strain 1886 | Streptococcus type strain 588 |
| Rhizoma Coptidis | 2.0 | 31.3 | 62.5 | ＞500 | 62.5 | ＞500 |
| Galla Chinensis | 2.0 | 250 | 62.5 | 62.5 | 31.3 | 62.5 |
| Flos Lonicerae | ＞500 | 250 | 125 | 125 | 62.5 | 500 |
| Fructus Gardeniae | 250 | 125 | 62.5 | 125 | 62.5 | 2.0 |
| Radix Salviae Miltiorrhizae | 15.6 | ＞500 | 500 | ＞500 | 500 | 500 |
| Cortex Phellodendri | 2.0 | ＞500 | ＞500 | ＞500 | ＞500 | ＞500 |
| Herba Taraxaci | 62.5 | ＞500 | 500 | ＞500 | ＞500 | ＞500 |
| Pericarpium Granati | 31.3 | 500 | ＞500 | ＞500 | ＞500 | 500 |
| Radix Gentianae | 500 | ＞500 | ＞500 | ＞500 | ＞500 | ＞500 |
| Radix Scutellariae | 3.9 | ＞500 | ＞500 | ＞500 | ＞500 | 31.3 |
| Radix Et Rhizoma Rhei | 3.9 | 250 | 125 | 500 | ＞500 | 125 |
| Fructus Chebulae | 31.3 | 250 | 62.5 | 500 | 250 | 250 |
| Cortex Fraxini | 31.3 | ＞500 | 2.0 | ＞500 | ＞500 | ＞500 |
| Radix Isatidis | 250 | ＞500 | ＞500 | ＞500 | ＞500 | ＞500 |
| Herba Taraxaci is loose | 62.5 | ＞500 | 125 | ＞500 | ＞500 | 250 |
| Chinese medicine fluid extract | 2.0 | 15.6 | 31.3 | 31.3 | 31.3 | 2.0 |
Result demonstration, in three kinds of Chinese medicines, Rhizoma Coptidis and Galla Chinensis are 2.0 mg/mL to staphylococcus aureus minimum inhibitory concentration (MIC); Galla Chinensis is 31.3mg/mL to streptococcus 1886 MIC; Fructus Gardeniae is 2.0mg/mL to streptococcus type strain 588 MIC; Rhizoma Coptidis is 2.0mg/mL to F streptococcus m IC; Galla Chinensis is 62.5mg/mL to D streptococcus m IC; Rhizoma Coptidis is 31.3mg/mL to B streptococcus m IC.But use Chinese medicine fluid extract to carry out extracorporeal bacteria inhibitor test, to many strains antibacterial, MIC all has in various degree and reduces.Result shows, extract prepared by Chinese medicine composition disclosed by the invention has obvious inhibitory or killing effect to mastitis pathogen, and effect is better than single medicine.
Finally explanation is, above preferred embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is described in detail by above preferred embodiment, but those skilled in the art are to be understood that, can make various changes to it in the form and details, and not depart from the claims in the present invention book limited range.
Claims (10)
1. the Chinese medicine composition of control mammitis of cow, is characterized in that: described Chinese medicine composition by Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis by weight forming for 2:2-4:2-1.
2. the Chinese medicine composition of preventing and treating according to claim 1 mammitis of cow, is characterized in that: described Chinese medicine composition by Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis by weight forming for 2:2:1.
3. one kind taking Chinese medicine composition described in claim 1 or claim 2 as the preparation of active component.
4. preparation according to claim 3, is characterized in that: described preparation is ointment.
5. preparation according to claim 4, is characterized in that: described ointment contains Rhizoma Coptidis, Fructus Gardeniae and the gallic fluid extract that percentage by weight is 10-15%.
6. preparation according to claim 5, is characterized in that: described ointment comprises that percentage by weight is 13% Rhizoma Coptidis, Fructus Gardeniae and Galla Chinensis fluid extract.
7. according to the preparation described in claim 5 or 6, it is characterized in that: the preparation method of Rhizoma Coptidis, Fructus Gardeniae fluid extract: get Rhizoma Coptidis and the Fructus Gardeniae of formula ratio, the ethanol extraction that is 75% by volume fraction, extracting solution is concentrated into medical material gross weight, obtains alcohol extract; Medicinal residues extracting in water, extracting solution is concentrated into medical material gross weight, obtains water extraction liquid, then merges alcohol extract and water extraction liquid, concentrated, obtains Rhizoma Coptidis, Fructus Gardeniae fluid extract.
8. preparation according to claim 7, it is characterized in that: the preparation method of Rhizoma Coptidis, Fructus Gardeniae fluid extract is: get formula ratio Rhizoma Coptidis and Fructus Gardeniae, add and be equivalent to the soak with ethanol 30 minutes that the volume fraction of 5 times of Rhizoma Coptidis and Fructus Gardeniae weight is 75%, then be reflux, extract, 2 times under 80 DEG C of conditions in temperature, each 1 hour, filtrate is concentrated into medical material gross weight, obtains alcohol extract; In filtering residue, add the water that is equivalent to 8 times of filtering residue weight, heating extraction 3 times is extracted 1 hour at every turn, collect filtrate, and filtrate being concentrated into is equivalent to medical material gross weight, obtain water extraction liquid, then merge alcohol extract and water extraction liquid, and be concentrated into and be equivalent to medical material gross weight, obtain Rhizoma Coptidis, Fructus Gardeniae fluid extract.
9. according to the preparation described in claim 5 or 6, it is characterized in that: the preparation method of Galla Chinensis fluid extract: the Galla Chinensis of getting formula ratio, add the water that is equivalent to 15 times of Galla Chinensis weight, be to extract 2 times under 100 DEG C of conditions in temperature, each 2 hours, then filtration collection filtrate be concentrated into and be equivalent to Galla Chinensis weight, obtains Galla Chinensis fluid extract.
10. the application of Chinese medicine composition in the medicine of preparation control mammitis of cow described in claim 1 or 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310124826.4A CN103191232B (en) | 2013-04-11 | 2013-04-11 | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310124826.4A CN103191232B (en) | 2013-04-11 | 2013-04-11 | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103191232A CN103191232A (en) | 2013-07-10 |
| CN103191232B true CN103191232B (en) | 2014-06-11 |
Family
ID=48714166
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310124826.4A Active CN103191232B (en) | 2013-04-11 | 2013-04-11 | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103191232B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105878439A (en) * | 2016-06-02 | 2016-08-24 | 隆昌锐智恒创生物科技有限公司 | Traditional Chinese medicine spray film agent for preventing and treating cow mastitis and preparation method thereof |
| CN114848680B (en) * | 2021-02-03 | 2023-07-25 | 四川好医生攀西药业有限责任公司 | Composition for treating cow mastitis and preparation method thereof |
-
2013
- 2013-04-11 CN CN201310124826.4A patent/CN103191232B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 中草药抗金黄色葡萄球菌的研究现状;陈燕丽等;《中兽医医药杂志》;20061231(第5期);26-28 * |
| 陈燕丽等.中草药抗金黄色葡萄球菌的研究现状.《中兽医医药杂志》.2006,(第5期),26-28. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103191232A (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102139030B (en) | Oral Chinese herbal preparation for treating gingivitis and preparation method thereof | |
| CN103110868B (en) | Skin care solution and preparation method thereof | |
| CN104435379B (en) | A kind of plant extract complex with antibacterial anti-inflammatory, hemostasis and pain relieving and application thereof | |
| CN104288356B (en) | Treat traditional Chinese medicine infusion solution of mastitis for milk cows and preparation method thereof | |
| CN105560372A (en) | Traditional Chinese medicine spray for treating rhinitis and preparation method thereof | |
| CN103800772A (en) | Gynecological external lotion for preventing and treating vagina inflammatory diseases and preparation method thereof | |
| CN102225127B (en) | Traditional Chinese medicine composite for treating mastitis of dairy cattle | |
| CN101167936B (en) | Natural plant extraction liquid for preventing cow mastitis and preparation method thereof | |
| CN102038748B (en) | Traditional Chinese medicine transdermal ointment for treating bovine mastitis and preparation method thereof | |
| CN104721241B (en) | A kind of Chinese Hibiscu petal extract and its application | |
| CN109620879A (en) | A kind of wet repairing paste and preparation method thereof clearly | |
| CN103191232B (en) | Traditional Chinese medicine composition for preventing and treating mastitis of dairy cattle and preparation and application of composition | |
| CN105943666B (en) | Composition for nursing sensitive skin of infant and preparation method thereof | |
| CN103054969B (en) | Compound skin disinfectant and preparation method thereof | |
| CN101780227A (en) | Traditional Chinese medicine composition for treating acute stroke and preparation method thereof | |
| CN104367746A (en) | Compound traditional Chinese medicine perfusion agent used for treating endometritis of dairy cow and preparation method of compound traditional Chinese medicine perfusion agent | |
| CN108853277A (en) | A kind of Traditional Chinese medicine disinfectant for acupuncture | |
| CN103251691B (en) | Chinese medicine formula for treating bovine mastitis as well as extract and preparation thereof | |
| CN103721138B (en) | A kind of traditional Chinese medicine for external application and its preparation method treating rosacea | |
| CN105816594A (en) | Medicinal composition for treating acne as well as preparation method and application of medicinal composition | |
| CN113616774B (en) | Antibacterial composition containing plant stem cells, maintenance antibacterial liquid and preparation method thereof | |
| CN102895488B (en) | A kind of Chinese medicine composition for the treatment of domestic animal keratitis | |
| CN1977894A (en) | Medicinal composition with anti-inflammation effect and its preparing method | |
| CN103239594B (en) | Topical pharmaceutical composition for preventing and treating canine acariasis disease and preparation method thereof | |
| CN105641265A (en) | Aloe combination drug used for treating acne, preparation thereof and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 402460 Chongqing Changyuan County of Rongchang city Xueyuan Road street, No. 160 Patentee after: Southwest University Address before: 400715 Chongqing Road, Beibei District No. 2 Patentee before: Southwest University |