CN102139030B - Oral Chinese herbal preparation for treating gingivitis and preparation method thereof - Google Patents

Abstract

The invention provides an oral Chinese herbal preparation for treating gingivitis, which comprises the following raw materials in parts by weight: 10-20 parts of angelica, 10-20 parts of indigo naturalis, 10-20 parts of salvia miltiorrhiza, 10-20 parts of cortex lycii radicis, 10-20 parts of radix notoginseng, 10-20 parts of radix angelicae, 10-20 parts of notopterygium root, 10-20 parts of areca catechu, 10-20 parts of honeysuckle, 10-20 parts of gardenia, 10-20 parts of scutellaria baicalensis, 10-20 parts of golden cypress, 10-20 parts of borneol, 10-20 parts of mint and 10-20 parts of patrinia. The Chinese herbal preparation is capable of clearing heat, removing toxin, relieving bruises and pains, promoting tissue regeneration, and astringing to arrest bleeding, has a better treatment effect on gingivitis, and has the advantages of rich medicine sources, low price, small side effect and the like; therefore, Chinese herbal mouthwash has a stronger clinical effect of combining all the medicines, and has the functions of clearing heat, purging fire, nourishing yin, falling fire, dredging collaterals, and relieving pain.

Description

A kind of oral cavity Chinese medicine preparation of treating gingivitis and preparation method thereof

Technical field

The present invention relates to contain the medicinal preparation that derives from plant, animal or mineral component, particularly a kind of oral cavity Chinese medicine preparation of treating gingivitis and preparation method thereof.

Background technology

Gingivitis is meant that dental tissue is under the effect of paathogenic factor and the acute and chronic inflammation that takes place.Medically will around and the oral mucosa that covers the prominent surface of around teeth and groove be referred to as gingiva.Because almost everyone all can occurrence degree and the chronic gingivitis that do not wait of scope in certain period in all one's life, have reason fully: gingivitis is human modal disease.Suffer from gingivitis, gums can be red and swollen, or bleeding is arranged.If realize that tooth is hemorrhage easily when brushing teeth, or tooth has the tenderness situation, just need see odontologist.Gingivitis has all kinds, but modal, sickness rate is the highest is the chronic simple gingivitis, this gingivitis is called dirty gingivitis, marginal gingivitis again, usually said gingivitis is exactly the chronic simple gingivitis.The chronic simple gingivitis is only invaded gingiva tissue, does not invade other periodontal tissue.This gingivitis is a kind of chronic inflammatory disease that causes owing to the bacterial plaque near the facing gum edge, and the cause of disease is clear and definite, and is more general in child and teenager; Gingivitis did not take place in 4-5 year in prevalence in the past usually about 70%-90%, great majority were since 5 years old; With advancing age, its prevalence and seriousness also increase gradually, reach the peak to adolescence; After the adolescence, the prevalence of gingivitis slowly descends with the just long of age, and adult's gingivitis prevalence is lower.What slight chronic simple gingivitis was invaded is high gum of trip and gum nipple, and severe patient can be invaded attached gingiva, labial teeth district, and is obvious with lower jaw labial teeth inflammation especially.Sting fruit or when brushing teeth, gingival hemorrhage.Even healthy gingiva is firmly brushed teeth, can not take place hemorrhage.Normal free gum is very thin and is close to facing that when gingiva had inflammation, the hyperemia of gum edge was rubescent, swelling, soft; Gum edge thickening, interdental papilla becomes blunt circle, is not close to facing; And the gingival sulcus intensification, the severe patient attached gingiva can be because of tissue edema, and stippling disappears; Surface-brightening, the gum edge can have erosion or granulation hyperplasia, the gingival pocket pyorrhea.Gingivitis further develops, and a large amount of blood capillary proliferations of gingiva are expanded, highly hyperemia, and a large amount of inflammatory cells and tissue fluid are oozed out, and cause gingiva loose, but the cover part corona, this moment, gingiva was dark red or dark red, and spy is examined very easily hemorrhage.The course of disease can cause gingival fiber propagation than the elder, the gingival hyperplasia projection, and this moment, gingiva color was slight rubescent or approaching normal, and quality is harder, and is hemorrhage less.

Primary disease is not invaded other periodontal tissue owing to only invade gingiva, so tooth does not become flexible, X-ray sheet inspection alveolar bone, periodontal membrane, cementum are no abnormal.Because the gingivitis cause of disease is clear and definite, dental plaque is the paathogenic factor of morbidity, and pathological changes only is confined to gingiva, therefore, removes the cause of disease, eliminates bacterial plaque, can obtain positive effect.State of an illness the lighter adopts oral prophylaxis (being commonly called as toothwash) thoroughly to remove tartar usually, and the control bacterial plaque, goes out thing treatment or oral antibiotic at red swelling of gingiva.The gingival hyperplasia person is taken place, then need implement gingivoplasty, promptly the cut-out gingiva recovers the gingiva physiology profile.Through above-mentioned treatment, after elimination of gingiva inflammation and gingiva form are recovered,, must adhere to conscientiously and rationally brushing teeth every day for keeping and consolidating curative effect, thoroughly remove dental plaque.Gingivitis is not as treating, and gingivitis continues development can invade deep periodontal tissue, develops into periodontitis.

Gingival hemorrhage often is the result of various focusing depths represented; Inflammation is again due to tartar, tartar stimulate; The formation of tartar, tartar is not have often thoroughly to remove the food debris and the calcareous institute that are deposited in teeth space and the gingival pocket to cause.Brushing teeth gargles is the most effectual way of removing these causes of disease.Chronic gingivitis is not have the result that brushes teeth correctly, frequently and gargle.The good method of prevention gingival hemorrhage is sooner or later to brush teeth effectively, gargles after meal.Gingivitis has all kinds, but modal, sickness rate is the highest is the chronic simple gingivitis, this gingivitis is called dirty gingivitis, marginal gingivitis again, usually said gingivitis is exactly the chronic simple gingivitis.

Generally acknowledge that at present gingivitis is an infectious disease, the main source of infection is the microorganism of dental plaque in neck portion and the gingival sulcus.Bacterial plaque microorganism and product long term thereof cause the immune response of body in gingiva, at first cause the inflammatory reaction of gingiva.When suffering from gingivitis, pathological changes is confined in gingival epithelium and the connective tissue.Be extended down to deep periodontal tissue when inflammation expands, can cause variations such as frontal resorption and pericemental dissolving, destruction.So control dental plaque effectively, the control gingivitis, what block periodontal disease is our primary work.Thoroughly remove tartar, smooth face meets biological requirement, makes gingival connective tissue might be attached to the root face again, forms and newly adheres to.Present market and a large amount of clinically Western medicine collutory that uses, spray etc., the chronic toxicity of chemicals often makes the patient give up halfway; Because patient occurred frequently is teenager, mouthfeel is poor, thereby curative effect is relatively poor; Hibitane the with a long history of hydraulic control bacterial plaque of gargling, effect is comparatively sure, but chlohexidine makes its clinical practice receive certain restriction because of having shortcomings such as the tooth of making dyeing, bitter in the mouth.Chinese herbal medicine has advantages such as the medicine source is abundant, price is low, side effect is little, so the Chinese medicine collutory has bigger clinical use prospect.

Summary of the invention

Technical problem to be solved by this invention is, a kind of quickly alleviating pain, heat-clearing and toxic substances removing are provided, reducing swelling and alleviating pain, and removing the necrotic tissue and promoting granulation, astringing to arrest bleeding has the Chinese medicine preparation of good therapeutic effect for gingivitis.Patient occurred frequently is teenager, and the problem of mouthfeel makes majority can not accept the long period and takes Western medicine.The chronic toxicity of chemicals the patient is given up halfway, thereby curative effect is relatively poor.Therefore, give full play to the Western medicine onset rapidly, the advantage of Chinese medicine persistent.The present invention uses different Chinese medicine characteristics, works out a kind of can price low, advantage such as side effect is little, and the merit of clearing away heat-fire, nourishing YIN to lower pathogenic fire, removing obstruction in the collateral to relieve pain; Heat-clearing and toxic substances removing, reducing swelling and alleviating pain, removing the necrotic tissue and promoting granulation, astringing to arrest bleeding has the Chinese medicine preparation of good therapeutic effect for gingivitis.

For solving the problems of the technologies described above, the invention provides a kind of oral cavity Chinese medicine preparation of treating gingivitis, the ratio of weight and number of various crude drug is in the said preparation: 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Indigo Naturaliss, 10～20 parts of Radix Salviae Miltiorrhizaes; 10～20 parts of Cortex Lycii, 10～20 parts of Radix Notoginseng, 10～20 parts of the Radixs Angelicae Dahuricae, 10～20 parts of Rhizoma Et Radix Notopterygiis; 10～20 parts of Semen Arecaes, 10～20 parts of Flos Loniceraes, 10～20 parts of Fructus Gardeniaes, 10～20 parts of Radix Scutellariaes; 10～20 parts of Cortex Phellodendris, 10～20 parts of Borneolum Syntheticums, 10～20 parts of Herba Menthaes, 10～20 parts of Herba Patriniae.

The oral cavity Chinese medicine preparation of said treatment gingivitis, the ratio of weight and number of various crude drug can be 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Indigo Naturaliss, 10～20 parts of Radix Salviae Miltiorrhizaes in the said preparation; 10～20 parts of Cortex Lycii, 10～20 parts of Radix Notoginseng, 10～20 parts of the Radixs Angelicae Dahuricae, 10～20 parts of Rhizoma Et Radix Notopterygiis; 10～15 parts of Semen Arecaes, 10～15 parts of Flos Loniceraes, 10～15 parts of Fructus Gardeniaes, 10～15 parts of Radix Scutellariaes; 10～15 parts of Cortex Phellodendris, 10～15 parts of Borneolum Syntheticums, 10～15 parts of Herba Menthaes, 10～15 parts of Herba Patriniae.

The oral cavity Chinese medicine preparation of said treatment gingivitis, the ratio of weight and number of various crude drug also can be 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of Indigo Naturaliss, 10～15 parts of Radix Salviae Miltiorrhizaes in the said preparation; 10～15 parts of Cortex Lycii, 10～15 parts of Radix Notoginseng, 10～15 parts of the Radixs Angelicae Dahuricae, 10～15 parts of Rhizoma Et Radix Notopterygiis; 10～20 parts of Semen Arecaes, 10～20 parts of Flos Loniceraes, 10～20 parts of Fructus Gardeniaes, 10～20 parts of Radix Scutellariaes; 10～20 parts of Cortex Phellodendris, 10～20 parts of Borneolum Syntheticums, 10～20 parts of Herba Menthaes, 10～20 parts of Herba Patriniae.

The oral cavity Chinese medicine preparation of said treatment gingivitis, said dosage form can include but are not limited to: suck liquid, buccal tablet, spray, powder, collutory, effervescent gargle sheet, slow releasing capsule, effervescent tablet or liniment.

For solving the problems of the technologies described above; The present invention also provides a kind of method for preparing of treating the oral cavity Chinese medicine preparation of gingivitis; When the dosage form of said preparation was collutory, its preparation method may further comprise the steps: Borneolum Syntheticum, Herba Menthae and Rhizoma Et Radix Notopterygii are extracted with steam distillation, get crystal and sucking filtration liquid; Sucking filtration liquid concentrate water extract a, the crystal dissolve with ethanol gets pure liquid b; After all the other flavour of a drug mixed the back decocte with water, water extract decompression thin film concentration to relative density 1.05～1.10 was used ethanol precipitation; Merge decompression thin film concentration to relative density 1.02～1.06 after the precipitate with ethanol supernatant concentration with above-mentioned water extract a, pure liquid b; Concentrated solution adds adjuvant, thin up, and packing promptly gets collutory.

In the said preparation process, above-mentioned water extraction process can for: decocting boils secondary, and for the first time 8～12 times of quality of medicinal material water decocted 1～3 hour, and the second time, 6～10 times of quality of medicinal material water decocted 1～3 hour.

In the said preparation process, alcoholic acid volumetric concentration can be 60%～80% in the above-mentioned ethanol precipitation, places 12～24 hours.

In the said preparation process, the concentration of the metronidazole that adds in the concentrated solution can be for diluting 50%～60% metronidazole.

For solving the problems of the technologies described above; The present invention provides a kind of method for preparing of treating the oral cavity Chinese medicine preparation of gingivitis again; When the dosage form of said preparation is that the method for preparing of liniment comprises: earlier said crude drug is ground into coarse powder except that Borneolum Syntheticum and Herba Menthae, further pulverizes again, mistake 120 mesh sieves; The fine powder amount that enable pass is crossed 120 mesh sieves is reaching 30%, and the fine powder of collecting through 120 mesh sieves gets fine powder a; Can not drop into multi-function extractor through the coarse powder of 120 mesh sieves again and extract twice, and add 8-10 times of water gaging for the first time and decocted 1-2 hour, and add 4-8 times of water gaging for the second time and decocted 1-2 hour, merge decocting liquid twice, and cross and filter filtrating; Heating is concentrated into pasty state, puts into 80 ℃ of oven dry of baking oven postcooling, grinds to form fine powder b, and is subsequent use after a is mixed with b, then Borneolum Syntheticum, Herba Menthae extracted with steam distillation, gets crystal c; C is added in the mixture of a and b, add 0.1%～0.5% Fluorinse and process liniment.

For solving the problems of the technologies described above, the present invention also provides a kind of method for using of treating the oral cavity Chinese medicine preparation of gingivitis, it is characterized in that, can directly powder of the present invention be put into toothpaste and use, also can be with keeping in the mouth after the powder bath; Liniment is directly smeared the gingiva place, and every day 3-5 time, embrocating 7 days continuously is a course of treatment; Collutory uses every day after meal, each 10ml～20ml, and rinsing the mouth was told in 3 minutes.

The maximum characteristics of the present invention are quickly alleviating pain, heat-clearing and toxic substances removing, reducing swelling and alleviating pain, and removing the necrotic tissue and promoting granulation, astringing to arrest bleeding has the oral cavity medicine of good therapeutic effect for gingivitis.The treatment characteristic of said preparation is Chinese medicine, giving consideration to both the incidental and fundamental.Control recurrent aphtha preparation good water solubility, the patient can take medicine under the rinsing the mouth posterior phraynx through special method of administration-stage of attack, thereby accomplished the combination of topical therapeutic and whole body therapeutic.Through Chinese medicine, giving consideration to both the incidental and fundamental reaches the recurrence of control aphtha, reduces recurrent number, prolongs recurrence period, alleviates the purpose of attack degree.Another treatment characteristic of said preparation is to lead under the supernatant, gives evil outlet.Control recurrent aphtha preparation can improve symptoms such as smelly, the vexed heating of heat, constipation with dry stool, yellowish or reddish urine in the mouth significantly, in both can having made strongly fragrant fire from last and clear, from outside and send out, can make it again to lead through under convenient to both.

The specific embodiment

Motherland's medical science is thought " tooth being the surplus of the bone ", and kidney governing bones loses the tooth person of coming off, and how by a little less than the deficiency of kidney-QI, QI and blood can not Rong Run, and bone is become homeless foster, so that tooth shakes is loosening; If through deficient and weak pulse, wind heat is attacked, and then causing swollen itching, in addition with gum between body fluid fight mutually, turn to pus or hemorrhage, its cause of disease and kidney, stomach are relevant, pathogenesis is a deficiency of the kidney void, qi depression to blood stasis, thereby select enriching yin and nourishing kidney, blood-activating and qi-promoting, clearing away heat and cooling blood medicine for use.Gingivitis belongs to traditional Chinese medical science gingival abscess, toothache, bleeding from the gum category.Pathogenesis is that positive bright stomach is through excess-fire or few high, hyperactivity of deficient fire of cloudy kidney channel deficiency of YIN fire.Research in recent years shows that Chinese medicine has certain curative effect to gingivitis.Find that in clinical observation only use clearing away heat-fire, nourishing YIN to lower pathogenic fire medicine, curative effect is undesirable.Should deposit in the cloudy medicine in the bitter cold pathogenic fire purging, the article of the diffusing fire of a small amount of hot temperature of adapted can be eradicated flourishing pathogenic fire with using corrigent.

The traditional Chinese medical science thinks that the pathogenesis of gingivitis mainly is the long-pending heat of stomach internal organs, and positive naked light is vehement, damp and hot fumigating in last and form gingival atrophy; Kidney unit is deficient, kidney governing bones, tooth being the surplus of the bone, deficiency of the kidney, can not on reach, tooth loses and to moisten fosterly, causes odontoseisis, gingival atrophy; Deficiency of qi and blood, gums lose supports, the pathogenic factor invasion, and the visitor causes gingival hemorrhage, atrophy, root of the tooth to appear, become flexible between cog.So Chinese medical discrimination to the understanding of its cause of disease reduce that the oral cavity is unclean, the long-pending heat of gastrointestinal, deficient qi and blood and kidney qi loss, being familiar with very early suffers from a deficiency of the kidney can cause periodontitis: says " institute of tooth person kidney is whole, and the institute of marrow is foster " in " General Treatise on the Cause and Symptoms of Diseases "; Qing Dynasty's Zhang Jingyue is thought " renal failure then tooth is fallen, and is smart in then tooth is hard " in Jing-Yue Complete Works; " support toothache down, belong to brothers Yang Ming two warps more, the event of gastric heat hyperactivity of fire " claimed in hoping of Qing Dynasty's force.

The treatment primary disease should be main with heat-clearing and toxic substances removing, YIN nourishing and the production of body fluid promoting, and assistant is with blood circulation promoting and blood stasis dispelling.The Radix Angelicae Dahuricae can clearing away heat and cooling blood, again can vital essence replenishing, and giving consideration to both the incidental and fundamental; The Radix Angelicae Sinensis clearing away heat and cooling blood, promoting blood circulation to remove blood stasis can strengthen the merit of Flos Lonicerae, removing toxicity for eliminating carbuncles eliminating stagnation; Radix Salviae Miltiorrhizae, the promoting blood circulation and cooling blood eliminating carbuncle is calmed the nerves; Radix Scutellariae, it is descending to ignite, to rush down the fire of steaming; Cortex Lycii, QI invigorating invigorating middle warmer, heat-clearing and toxic substances removing, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.Modern study proves: Radix Salviae Miltiorrhizae can suppress radical reaction, reduces the LPO nucleus formation, suppresses staphylococcus aureus, helicobacter pylori, osteomiosis complementary energy antiviral and two-ways regulation immunity of organisms.Rhizoma Et Radix Notopterygii and Borneolum Syntheticum causing resuscitation with aromatic drugs, the pain relieving dissipating blood stasis.Semen Arecae, metronidazole have stronger bactericidal action.The Semen Arecae water decoction has inhibitory action to streptococcus mutans killer cell adhesion.Cortex Phellodendri etc. to the growth of Streptococcus mutans, produce acid and sticking of hard thing certain inhibitory action arranged.Flos Lonicerae in the Chinese medicine, Herba Menthae have the effect of reducing swelling and alleviating pain.Herba Menthae can be eliminated the bitterness in the Chinese medicine, and makes people's breath freshening, and the mouthfeel when gargling is fine.Metronidazole has antibacterial activity to anaerobe, can penetrate microorganism, and destruction DNA of bacteria bacterium chain perhaps suppresses it and synthesizes.Chinese herbal medicine has advantages such as the medicine source is abundant, price is low, side effect is little, share so the Chinese medicine collutory has bigger clinical making at medicine, plays the merit of clearing away heat-fire, nourishing YIN to lower pathogenic fire, removing obstruction in the collateral to relieve pain altogether.

Radix Scutellariae has heat clearing and damp drying, and removing heat from blood is antiabortive, detoxicating functions.Cure mainly diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.The clinical practice of Radix Scutellariae is antibiotic not worse than Rhizoma Coptidis, and does not develop immunity to drugs.We are by the strong characteristics of broad-spectrum antibacterial action, and it is special severe to be used in the fungal culture microbial contamination, fine with Radix Scutellariae extracting solution effect." Bencao Jingshu ": Radix Scutellariae, its property is respectful clearly, so remove evil: so bitter in the mouth dampness; So YIN-cold wins heat, so main all heat.All hot persons, pathogenic heat with all over heat also, jaundice, intestinal be out-of-the-way, let out dysentery, the disease of all damp and hot victory is also analysed it, and is then all sick from recover.Bitter cold can be removed damp and hot, thus small intestinal profit and water from pursuing, the source is clearly then flowed also clean.The blood person of closing, excess-heat is at blood system, instant heating human blood chamber, it is obstructed to make us amenorrhea, damp and hot separating, then Ying Qi is clearly and voluntarily also.Malignant boil cellulitis erosion person, heat in blood is then stayed knot, and is that carbuncle stain is mashed also: the acute scleritis person, the fire of moxibustion blood trouble also, the removing heat from blood heat extraction, then spontaneous recovery also.Radix Scutellariae has antiallergic action and antiinflammatory action: contained baicalin of Radix Scutellariae and baicalin can obviously suppress sensitized guinea pig rat small intestine in vitro and isolated tracheal to anaphylaxis contractile response that antigen produced; To passive general allergy and the Cavia porcellus passivity skin allergic reaction of Cavia porcellus and mice, baicalin and baicalin also have inhibitory action.Radix Scutellariae has refrigeration function, most evidences, and the active ingredient baicaligenin of Radix Scutellariae has refrigeration function.Radix Scutellariae has Detoxication, and baicalin has tangible Detoxication, and this possibly be because under the effect of glucuronidase, and baicalin decomposites glucuronic acid, and with due to multiple poisonous substance combines.10 milligrams of LD 50 that can improve strychnine of baicalin reach 2.5 times, and the hepatic glycogen content of carbon tetrachloride poisoning mice is increased.

Radix Salviae Miltiorrhizae is bitter, is slightly cold.GUIXIN, Liver Channel.Stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, relieving restlessness clears away heart-fire.Be used for menoxenia, the amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia.Be used for the costa sternales hypochondriac pain, rheumatic arthralgia, lump in the abdomen caking, skin infection swells and ache, tumbling down is grieved, menoxenia, the amenorrhea dysmenorrhea, puerperal stasis of blood pain etc.Treatment costa sternales pain, lump in the abdomen caking, and menoxenia, amenorrhea dysmenorrhoea have good effect.. " detailed outline " carries: Radix Salviae Miltiorrhizae, by " the sensible opinion of married woman " cloud, SIWU TANG is controlled the gynecopathy, pays no attention to antenatal puerperal, through water what, all can be general, only flavor Radix Salviae Miltiorrhizae looses, and cures mainly identical with it.The lid Radix Salviae Miltiorrhizae can break stagnated blood, mends fresh blood, peaceful tire, and the stillborn fetus that falls ends the metrorrhagia dysentery, the regulating menstruation arteries and veins.Radix Salviae Miltiorrhizae main component TANSHINONES has blood vessel dilating, improves effects such as circulating, remove intravascular coagulation, has the effect of invigorating blood circulation trace, and can promote the liver blood circulation.

Indigo Naturalis is hot in nature, warming middle-JIAO, and pain relieving is regulated the flow of vital energy, dampness, the contrary acid regurgitation of stopping vomiting, headache due to JUE YIN disorder, the coldness of ZANG-organ is vomited and diarrhoea, abdominal distention, abdominal pain during menstruation is had loose bowels just before dawn, vascular hypertension, beriberi, hernia, aphthous ulcer, toothache, dermatomycosis.Acrid in the mouth, hardship, dispersing cold for relieving pain is gone into liver, spleen, stomach, large intestine, kidney channel.External has apparent in view pain relieving and therapeutic effect." Thunder God is concocted the property of medicine and separated ": warming middle-JIAO, pain relieving is regulated the flow of vital energy, dampness." herbal classic " carries: main warming the middle warmer and descending qi, and pain relieving, the cough with dyspnea cold and heat, the dehumidifying arthralgia due to stagnation of blood, by ailment said due to cold or exposure, loosing the interspaces of skin and muscles is the good medicine of externally used detumescence.

Rhizoma Et Radix Notopterygii property is hot, bitter in the mouth, temperature.Return bladder, kidney channel.Function cures mainly cold expelling, dispels the wind dehumidifying, pain relieving.Be used for the anemofrigid cold headache, rheumatic arthralgia, aching pain in should and back.Be used for anemofrigid cold, the headache body is painful.The hot temperature of these article, gas is male and loose, and the power of delivering is strong; The heresy of main loose Taiyang channel ailment said due to cold or exposure and cold-damp; Have that cold expelling dispels the wind, the merit of removing dampness to relieve pain, so kindly control wind-cold damp pathogen and attack table, fever with aversion to cold, the flesh table is lossless, headache stiffness of the nape, limb aching pain person; The same usefulness of medicine such as normal and Semen Persicae, Rhizoma Et Radix Notopterygii, Radix Clematidis, Rhizoma Chuanxiong are like Notoptreygium Decoction of Nine Ingredients; If cold-damp lays particular stress on, headache heavy sensation of the body person, but medicines such as compatibility Radix Angelicae Pubescentis, manuscript, Rhizoma Chuanxiong are like Decoction of Notopterygium for Rheumatism.The Rhizoma Et Radix Notopterygii tanginess is to make the good volatile oil of drop pill.

The sweet mildly bitter flavor of Radix Notoginseng property, warm in nature, return liver, stomach through being used as medicine with root, root stock.Be rare Chinese medicine, but give birth to have good hemostasia effect, significant hemopoietic function with hemostasia and dissipation blood stasis, reducing swelling and alleviating pain; Can tighten and improve the arteria coronaria microcirculation, Radix Notoginseng is used as medicine with a long history, acts on peculiarly being regarded as the treasured in the medicine by ancient Chinese medicine doctor, so the saying of " Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) " is arranged." it is little sweet and bitter to distinguish the flavor of, quite seemingly the flavor of Radix Ginseng for Radix Notoginseng." " all canes are pounced on trauma, and the dripping person of blood stasis chews promptly ending of mashed net for catching birds or fish immediately, and bruise the person promptly dissipate.If when receiving cane, clothes one, two money earlier, then the blood centre of punch not should be obeyed it behind the cane especially, and obey also good puerperal.On the whole this medicine temperature, sweet and slightly bitter taste, and sun is bright, the medicine of the cloudy blood system of fainting, so can control all disorders of blood "." book on Chinese herbal medicine is looked for the truth " record: " Radix Notoginseng, common people only know the function hemostasis and pain-relieving.Hardly realize bitterly and ache work that blood ends because of applying the blood that looses because of blood stasis.The bitter temperature of Radix Notoginseng abnormal smells from the patient can be in its blood stasis of blood systemization.”

The Flos Lonicerae heat clearing away is loose malicious, and Compendium of Material Medica is carried " Flos Lonicerae is good at removing toxic substances, so control carbuncle, toxic swelling, skin ulcer tinea ... ".Since ancient times, Flos Lonicerae is usually used in heat-clearing and toxic substances removing, the heating of treatment epidemic febrile disease, toxic-heat and blood stasis, diseases such as carbuncle and ulcer.Modern pharmacological research shows, that Flos Lonicerae has is antibacterial, antiviral, antiinflammatory, analgesic, regulate the effect traditional Chinese medical science such as immunity and think, Flos Lonicerae is cold in nature, sweet in the mouth, gas are put down, and has the effect of heat-clearing and toxic substances removing, can treat diseases such as pyretic toxicity abscess, carbuncle furuncle.And have the diffusing effect of a surname concurrently.The Flos Lonicerae antipyretic and antidote functions is quite strong, is article commonly used in the orthopaedic surgical operations.

Cortex Phellodendri cures mainly heat clearing and damp drying, and pathogenic fire purging removes steams, and skin ulcer is treated in detoxifcation." Chinese pharmacopoeia is introduced the power of Cortex Phellodendri heat clearing and damp drying, and is similar with Radix Scutellariae, Rhizoma Coptidis, but is good to remove the damp and hot of the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, is the good medicine of controlling damp and hot skin infection.

Borneolum Syntheticum is used for conjunctival congestion and swelling pain, the sore throat aphtha.The Borneolum Syntheticum bitter cold has the merit of clearing away heat to alleviate pain, detumescence, is ENT dept.'s medicine commonly used.Treatment laryngopharynx swelling and pain, aphtha of the mouth and tongue, normal and Borax, Cinnabaris, Matrii Sulfas Exsiccatus grind to the powder altogether, blow deposited affected part, like BINGPENG SAN.The treatment acute pharyngitis due to wind-heat is used for skin infection and swells and ache, and does not hold back after bursting.These article also have heat-clearing and toxic substances removing, erosion prevention and muscle growing effect.It is the good medicine good merchantable brand of external curing oral ulcer.

The Radix Angelicae Dahuricae is warm in nature, and the acrid in the mouth function cures mainly wind-damp dispelling, the evacuation of pus of invigorating blood circulation, granulation promoting pain relieving.Be used for headache, toothache, nasal sinusitis, hemorrhoidal hamorrhage anal fistula, leucorrhea with red and white discharge, ulcer sores, skin pruritus.Antipyretic effect: with the hyperpyrexia animal model that the peptone subcutaneous injection causes in the White Rabbit back, the 1g Radix Angelicae Dahuricae or Radix angelicae dahuricae decoct 15g/kg have tangible refrigeration function.The mouse stomach Radix Angelicae Dahuricae or Radix angelicae dahuricae decoct 8g/kg obviously reduce the mouse writhing number of times due to lumbar injection 1% acetic acid (0.1/kg), and effect is similar with aminophenazone 8mg/kg, P＜0.001.Woolfe-Macdonald method test, the preceding threshold of pain of administration 30 minutes with interior person, irritate stomach and award the Radix Angelicae Dahuricae or Radix angelicae dahuricae 8g/kg, after the administration 60 minutes, the Radix Angelicae Dahuricae and the anti-Radix Angelicae Dahuricae obviously improved pain threshold, with normal saline group P＜0.001 and 0.01 relatively." property of medicine opinion ": control the twinge of trusted subordinate's blood, exorcising, main woman's metrorrhagia and vomiting make eye bright, arresting lacrimation goes out, and treats married woman's drop blood, waist stomachache; Can lose pus.

Cortex Lycii is the root bark of matrimony vine of solanaceae plant Lycium chinense Mill..Can be used as medicine, have removing heat from blood and remove effects such as treasure, lung heat clearing pathogenic fire reducing.Heat clearing away, removing heat from blood.Control asthenia hectic fever night sweat, the lung-heat cough with asthma is spitted blood, epistaxis, and stranguria with blood is quenched one's thirst, hypertension, carbuncle, malignant boil." herbal classic ": " pathogen in main five pines for quenching one's thirst general arthralgia." " not Lu ": main air be wet, lower breast side of body gas, and internal injury Da Lao suctions of hissing mended in the exopathogenic heat headache, hard muscle, reinforcing YIN-essence, sharp intestine and small intestine, summer-heat resists cold.

The fruit of Fructus Gardeniae is a Chinese medicine, belongs to the 1st batch of medicine-food two-purpose resource of Ministry of Public Health promulgation, has effects such as hepatoprotective, function of gallbladder promoting, blood pressure lowering, calmness, hemostasis, detumescence, function removing dampness promoting tissue regeneration and ulcer healing.Be used for ulcer being unable to heal, eczema, the treatment of aphtha more.These article function clearing away heat-fire, removing pathogenic heat from blood and toxic substance from the body are availablely controlled pathogenic fire,toxin and furuncles, red and swollen heat pain person, often join Flos Lonicerae, Fructus Forsythiae, Herba Taraxaci and use; Or join the Radix Angelicae Dahuricae helping detumescence, as the poison that contracts looses." book on Chinese herbal medicine just ": " Fructus Gardeniae, if make with assistant, controlled difference: add Herba Artemisiae Scopariae and remove damp and hot jaundice, adding Semen Sojae Preparatum, to remove heart-fire irritated, adds Cortex Magnoliae Officinalis, Fructus Aurantii Immaturus can remove stuffy sensation with restlessness, with Rhizoma Zingiberis Recens, Pericarpium Citri Reticulatae can be dirty except that vomitting, and breaks the heat stagnation abdominal pain due to blood stasis with Rhizoma Corydalis.”

The Semen Arecae bitter in the mouth; Hot; Warm in nature.Return stomach; Large intestine channel.Effect cures mainly, anthelmintic; Removing food stagnancy; The therapeutic method to keep the adverse QI flowing downwards; Row water; Preventing the attack (or recurrence) of malaria.Malnutrition due to parasitic infestation; Dyspepsia; Abdominal distention; Heavy behind the dysentery; Foot gas; Edema; Malaria.Resisting pathogenic microbes effect Semen Arecae infusion (1: 1) in vitro to trichophyton, permitted dermatophytes such as blue achorion inhibitory action in various degree arranged.Some strain has certain inhibitory action to the influenza virus A type for decoct and water logging agent, and antiviral effect maybe be relevant with wherein contained tannin.

Radix Angelicae Sinensis, its sweet in the mouth and weighing, so specially can enrich blood, its gas is light and hot, thus again can promoting the circulation of blood, invigorating middle warmer has, in the row benefit is arranged, the gas medicine in the sincere blood, the also panacea in the blood.About mending of assistant to mend then, thus can support nutrition blood, the QI invigorating spermatogenesis, settling five organs, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack then, so the relieving constipation bitterly of dispelling, relieving rigidity of muscles and joints is controlled contracture, paralysis, card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, assistant is with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can loose greatly, and show not hold back the person, and help with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, so desire its quiet person when keeping away it, the sliding benefaction of property is defecated and is not consolidated the person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.

Herba Menthae is one of Chinese conventional Chinese medicine.It is hot cold property sweating and heat resolving medicine, controls influenza, headache, conjunctival congestion, fever of the body, throat, dental bed disease such as swell and ache.But the place is with training the mind dysmenorrhoea, skin pruritus, erythra and eczema etc.All herbal medicine, suffering, cold.Return lung, Liver Channel; The wind heat of dispersing is arranged, clearing throat, rash detoxifcation; Dispersing the stagnated live-QI to relieve the stagnation of QI and effect such as antipruritic; Be applicable to that cold, fever, headache, laryngopharynx swelling and pain, lossless, wind-fire acute conjunctivitis, rubella, skin are itched, disease such as colic, dysentery and scrofula, external has slight analgesic effect, is used for neuralgia etc.

The Herba Patriniae nature and flavor are bitter flat, have the effect of heat-clearing and toxic substances removing, evacuation of pus removing blood stasis with potent drugs.Can cure acute appendicitis, dysentery, leucorrhea with red and white discharge, the stagnant stomachache of the stasis of blood in puerperal, conjunctival congestion and swelling pain.Go into stomach, large intestine, Liver Channel.Cure mainly heat-clearing and toxic substances removing, eliminating carbuncle evacuation of pus, blood circulation promoting competent silt.Be used for acute appendicitis, lung abscess and sore and toxic, the breast abdomen pain due to the excess-heat stasis of blood stagnates, the stagnant stomachache of the stasis of blood in puerperal waits disease.

The manufacturing process that the present invention sucks agent is: get each raw medicinal material, Radix Angelicae Sinensis 1000g, Indigo Naturalis 1000g, Radix Salviae Miltiorrhizae 1000g, Cortex Lycii 1000g; Radix Notoginseng 1000g, Radix Angelicae Dahuricae 1000g, Rhizoma Et Radix Notopterygii 1000g, Semen Arecae 1000g, Flos Lonicerae 1000g; Fructus Gardeniae 1000g, Radix Scutellariae 1000g, Cortex Phellodendri 1000g, Borneolum Syntheticum 1000g, Herba Menthae 1000g; Herba Patriniae 1000g, water washing is used in selection, drying, it is subsequent use to process clean medical material; Get the clean medical material decocte with water three times of respectively distinguishing the flavor of, 6～10 times of water gagings soak each time, decoct, and filter; Add for the second time 3～7 times of water gagings, decoct, filter, add 2～6 times of water gagings for the third time, decoct; Filter 1.10～1.15 extractum when discarded medicinal residues, merging filtrate, reduction vaporization are concentrated into relative density and are 60～70 ℃; Add 95% ethanol and make that to contain alcohol amount be 70%, left standstill 24 hours, get supernatant, reclaim ethanol and be concentrated into relative density 1.15～1.18 concentrated solutions when being 60～70 ℃; Concentrated solution is sterilized with CO60, irradiation dose 8k, the vacuum freezing sealing promptly gets then.

Collutory of the present invention prepares process: Borneolum Syntheticum 1000, Herba Menthae 1000g and Rhizoma Et Radix Notopterygii 1000g are extracted with steam distillation, crystal and sucking filtration liquid, sucking filtration liquid concentrated water extract a, the crystal dissolve with ethanol gets pure liquid b; Radix Angelicae Sinensis 1000g, Indigo Naturalis 1000g, Radix Salviae Miltiorrhizae 1000g, Cortex Lycii 1000g, Radix Notoginseng 1000g; Radix Angelicae Dahuricae 1000g, Semen Arecae 1000g, Flos Lonicerae 1000g, Fructus Gardeniae 1000g, Radix Scutellariae 1000g; Cortex Phellodendri 1000g, Herba Patriniae 1000g mixes the back decocte with water, and decocting boils secondary; 8～12 times of quality of medicinal material water decocted 1～3 hour for the first time, and 6～10 times of quality of medicinal material water decocted 1～3 hour for the second time.Water extract decompression thin film concentration to relative density 1.05～1.10 is used ethanol precipitation; Alcoholic acid volumetric concentration is 60%～80% in the ethanol precipitation, places 12～24 hours.Merge decompression thin film concentration to relative density 1.02～1.06 after the precipitate with ethanol supernatant concentration with above-mentioned water extract a, pure liquid b; Concentrated solution adds metronidazole, and the concentration of the metronidazole that adds in the concentrated solution is dilution 50%～60% metronidazole.Behind the thin up, packing promptly gets collutory.

The preparation process of liniment of the present invention is: get Radix Angelicae Sinensis 1500g, Indigo Naturalis 1500g, Radix Salviae Miltiorrhizae 1500g, Cortex Lycii 1500g, Radix Notoginseng 1500g; Radix Angelicae Dahuricae 1500g, Rhizoma Et Radix Notopterygii 1500g, Semen Arecae 1500g, Flos Lonicerae 1500g, Fructus Gardeniae 1500g; Radix Scutellariae 1500g, Cortex Phellodendri 1500g, Herba Patriniae 1500g is ground into coarse powder; Further pulverize, cross 120 mesh sieves, the fine powder amount that enable pass is crossed 120 mesh sieves is reaching 30%, and the fine powder of collecting through 120 mesh sieves gets fine powder a; Can not drop into multi-function extractor through the coarse powder of 120 mesh sieves again and extract twice, and add 8-10 times of water gaging for the first time and decocted 1-2 hour, and add 4-8 times of water gaging for the second time and decocted 1-2 hour, merge decocting liquid twice, and cross and filter filtrating; Heating is concentrated into pasty state, puts into 80 ℃ of oven dry of baking oven postcooling, grinds to form fine powder b, and is subsequent use after a is mixed with b, then Borneolum Syntheticum 1500g, Herba Menthae 1500g extracted with steam distillation, gets crystal c; C is added in the mixture of a and b, add 0.1%～0.5% Fluorinse and process liniment.

Spray preparing process of the present invention comprises: with said crude drug Radix Angelicae Sinensis 1500g, Indigo Naturalis 1500g, Radix Salviae Miltiorrhizae 1500g, Cortex Lycii 1500g, Radix Notoginseng 1500g; Radix Angelicae Dahuricae 1500g, Rhizoma Et Radix Notopterygii 1500g, Semen Arecae 1500g, Flos Lonicerae 1500g; Fructus Gardeniae 1500g, Radix Scutellariae 1500g, Cortex Phellodendri 1500g, Herba Patriniae 1500g; Add the water logging bubble 30 minutes to 45 minutes that 5-7 doubly measures, heating was boiled 30～40 minutes, filtered filtrate for later use; Filtering residue adds the water that 5-7 doubly measures and heats for the second time, boils 30～40 minutes, filters filtrate for later use; Filtering residue adds the water that 5-10 doubly measures again, and heated and boiled is 40～50 minutes for the third time, filters; Merge three times decoction liquor, be concentrated into relative density and be 1.08 concentrated solution; Filter, left standstill 24 hours; Then Borneolum Syntheticum 1500g, Herba Menthae 1500g are extracted with steam distillation, get crystal, the ethanol mixture of putting into concentrated solution and 10 times of amounts soaked 10 days, in the medical spray gun of packing into.

Pharmacology's toxicity test

The acute toxicity testing data

One, test material:

Animal: Kunming mouse, body weight 21-24g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: the present invention sucks agent, contains 0.365g crude drug/10g.

Two, method:

1, LD50 calculates: adopt the improvement karber's method, mice is divided into 5 groups at random, and 10 every group, male and female half and half; With the dissolving of powder adding distil water, be made into Cmax, by the administration of the maximum permission capacity of mice, institute is followed successively by 18 to dosage by the crude drug amount; 14.4,11.5,9.2,7.4 (g.kg-1); In animal fasting (can't help water) after 18 hours, administration at twice (half an hour at interval) in a day, each 0.5ml observes the animal dead situation.

2, maximum tolerated dose is measured (MTD value): get 20 of mices, each 10 of male and female.To suck agent adding distil water dissolving, be made into maximum concentration, and, irritate with injection and to feed device and can twitch and be as the criterion by the maximum tolerated dose of animal.In animal fasting (can't help water) after 18 hours, administration at twice (half an hour at interval) in a day, each 0.5ml (every ml contains crude drug 0.36g), total dose is 18g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Observed 7 days continuously after the administration.

Three, result of the test:

In LD50 calculates when giving mice (18g/Kg.d) with maximum acceptable concentration and maximum permission capacity; Do not see dead mouse, promptly do not measure LD50, ask maximum tolerated dose only; In 7 day observation period; Its appetite of animal, activity, hair color, the mental status etc. are all normal, grow normally, and not seeing has death.Promptly select 300 times of doses that are equivalent to clinical dosage for use, and have no adverse reaction, show that acute toxicity is minimum, MTD＞18g/Kg.d.

The long term toxicity test data

One, experimental technique

Cavia porcellus at random part is become 4 groups, 15 every group.In guinea pig back spinal column both sides depilatory is evenly coated, made about 40 square centimeters of its depilation scope.Clean depilatory; Observe that every group of Cavia porcellus is coated with liniment 0.2,0.4 of the present invention and 0.8ml respectively after 24 hours; Contain crude drug 92,184 and 368mg respectively, another group is coated with solvent 0.8ml secondary every day, continuous 30 days; Observe the ordinary circumstance of Cavia porcellus, experiment finishes back execution animal and carries out hematology, blood biochemical and pathological examination.

Two, result

Above-mentioned three groups of medication Cavia porcellus trunk QUMAO districts do not see that local skin has edema, hyperemia, erythema, petechia and ulcer.Medication group and control animals chroma of hair, ingest, matched group no significant difference such as extremity activity, blood biochemistry checking, medication group and matched group are all in normal range.Histopathologic examination, experiment is respectively organized the heart, liver, kidney and local skin and is not all seen obvious pathological changes.Prompting, Chinese medicine preparation long-term prescription of the present invention is not all seen tangible toxic action to local skin and whole body important organ.Result of the test shows: Chinese medicine preparation experiment safety of the present invention.

The oral mucosa irritant test:

Collutory of the present invention is applied to the rat oral mucosa; Every day 4 times, successive administration 4 days, two administration group dosage are respectively 40ml/kg.d (being equivalent to crude drug amount 10g/kg.d), 20ml/kg.d (being equivalent to crude drug amount 5g/kg.d); 4 days none animal deads after the administration; Rat diet, activity and defecation are all normal, and check behind the execution animal: oral mucosa is not found hyperemia, swelling, and liver, kidney, lung, gastrointestinal are also no abnormal.Histopathologic examination, high dose medicine group and matched group oral mucosa be no significant difference relatively.Explain that collutory of the present invention is to rat oral mucosa nonirritant.

The cumulative toxicity experiment:

Chinese medicine of the present invention suck agent to mice by 7.69,19.18 and the 43.21g crude drug/15 weeks of kg continuous use (1.0ml/100g body weight; Every day 2 times) and after 3 week of drug withdrawal; The result shows: Chinese medicine preparation of the present invention does not all have obvious influence to indexs such as the hair of rat, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat; The internal organs naked eyes do not find that difference changes and the histological examination result shows; After 15 weeks of medication and 3 weeks of drug withdrawal, each internal organs of rat all do not have obvious change.Explain that toxicity is little after Chinese medicine preparation oral capsule of the present invention is to the rat long-term prescription, also do not have the difference reaction after the drug withdrawal, application safety.

Skin and the test of body internal stimulus property:

Get rabbit and shave hair, use medicament liniment of the present invention to be example, induce and excite contact test, viewing test rabbit after 24 hours and 48 hours, all loseing skin has phenomenons such as erythema and edema, with the negative control group zero difference.The result shows that this liniment is bordering on zero to dermoreaction intensity, does not promptly have skin allergic reaction.The present invention's wiping is contained agent smear mouse vagina, induce and excite contact test, viewing test mouse vagina after 24 hours and 48 hours, all loseing skin has phenomenons such as erythema and edema, with the negative control group zero difference.The result shows that this liniment is bordering on zero to dermoreaction intensity.

The pharmacological experiment explanation:

The test of pesticide effectiveness

1, antiinflammatory action

One, the present invention sucks agent to pathology morphology of Golden Hamster oral ulcer and the exponential influence of immune organ due to the carbolic acid

100 of the yellow susliks of depletion are divided into 5 groups, 20 every group at random.Except that model control group, each treated animal gave the corresponding treatment medicine in preceding 36 hours, 12 hours in counteracting toxic substances.During counteracting toxic substances,, pull out the right side cheek pouch gently with two mosquito forcepss with the etherization Golden Hamster; Fixing, with the glass tubing of a long 6cm, internal diameter 0.8cm, a built-in cotton pellet; The bottom and the glass tubing end opening of cotton pellet are made even; Then in pipe, splash into 90% carbolic acid solution till just soaked into cotton pellet, then will in have the glass tubing lower end of carbolic acid cotton balls to be flat on the buccal mucosa at 2mm place in the bicker of Golden Hamster right side, burnt for 45 seconds.The counteracting toxic substances continued is by dividing into groups to give corresponding treatment medicine or normal saline.(1) model group: only give normal saline 5ml/; (2) the present invention sucks the agent high dose group: give the present invention and suck agent 10.8g crude drug/kg body weight (be equivalent to people's clinical medicine dose 10 times); (3) the present invention sucks dose groups in the agent: give the present invention and suck agent 5.4g crude drug/kg body weight (be equivalent to people's clinical medicine dose 5 times); (4) the present invention sucks the agent small dose group: give the present invention and suck agent 2.7g crude drug/kg body weight (be equivalent to people's clinical medicine dose 2.5 times); (5) positive drug matched group: give kouyanqing granules 3.5g/kg body weight (be equivalent to people's clinical medicine dose 5 times); Each treated animal is all irritated stomach group medicine, and the administration volume is 5ml/, and 1 time/day, continuous 7 days.Observe mouse immune organ index, the result sees table 1.

Table 1 Golden Hamster immune organ influences index (X ± S)

Two, the 1. influence of xylol induced mice auricle edema

Laboratory animal, number of animals, grouping, dosage and the same experiment of administration natural law.Difference is that immediately in the wide melted paraxylene 0.02ml of every Mus auris dextra, mice was put to death in the cervical vertebra dislocation after 20 minutes by the blue normal saline 0.1ml/10g of mouse tail vein injection 0.5% ivens body weight in 1 hour after the last administration; Cut the auris dextra exterior feature, downcut with the position homalographic, claim that with analytical balance ear is heavy with 9mm card punch ears; Heavily deducting left ear with auris dextra heavily is the swelling degree; And be soaked in after every Mus two auricles are shredded in 7: 3 acetone physiologic dose brines 48 hours, by last experimental technique colorimetric, the result sees table 2.

Table 2 the present invention sucks the X ± SD that influences of liquid xylol induced mice auricle edema

The result shows: the present invention contains liquid height, middle dose groups and blank group relatively has significant differences.

2, analgesic activity

(1) hot plate stimulus method, 17 ℃ of room temperatures, hot plate stimulates 55 ± 0.5 ℃ of constant temperature; Licking hind leg with mice is the pain index, and grouping before measurement mice pain threshold 2 times is selected 50 of the female mice of pain threshold in 30 seconds; Divide 5 groups at random by body weight and pain threshold: the present invention sucks liquid, and to establish 3 dose groups the same, positive controls (aspirin) 3.5g/kg body weight (be equivalent to people's clinical medicine dose 5 times), and model control group; Gastric infusion dosage is seen table 3; Every day 1 time, successive administration 3 days was respectively surveyed the mice pain threshold 1 time in 1 hour, 2 hours 3 hours after each administration.Experimental result is seen table 3.

Table 3 the present invention sucks the influence X ± SD of liquid to the mice pain threshold

Annotate: medicine group and matched group be * P＜0.05 relatively, * * P＜0.01.Can know from table 3,

Each group of this medicine more all has significant differences (P＜0.01 or P＜0.05) with the blank group.

Three, the present invention sucks agent to effect of immunologic function

1, the present invention sucks the influence of agent to the lesser sac macrophage phagocytic function

Get 80 of mices, body weight 18～22g, male and female half and half are divided into 5 groups at random.Each group is pressed table 2 dosage, is pressed the 20ml/kg gastric infusion respectively, once a day, and continuous 15 days.After the last administration 1 hour, every mouse peritoneal was injected 10% peptone 0.2ml, and pneumoretroperitoneum was injected 2% fresh chicken red blood cell 0.2ml in 32 hours; After 30 minutes, take off cervical vertebra and put to death mice, open the abdominal cavity; Wash out peritoneal fluid, put in the test tube, gentle 15min in 37 ℃ of water-baths; Shake once at a distance from 5min, centrifugal, abandon supernatant.Get the red cell suspension smear, behind the natural drying, use formaldehyde fixed 10min, reuse Ji nurse Sa dyeing 7min, oily mirror is 200 macrophages of counting down.With the macrophage phagocytic chicken red blood cell engulf percent and phagocytic index is that index is represented the influence of medicine to macrophage phagocytosis of mice.The result sees table 4-1.

Table 4-1 is to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic function (X ± S)

Annotate: compare * P＜0.05, * * P＜0.01 with blank control group.

Experimental result shows: what the present invention sucked that three dose groups of agent all can obviously improve Turnover of Mouse Peritoneal Macrophages engulfs percent and phagocytic index; Compare with blank control group; Significant difference (P＜0.05, P＜0.01) is arranged, and the present invention sucks the phagocytic activity that agent can strengthen Turnover of Mouse Peritoneal Macrophages.

Clinical data:

Physical data

30 examples are organized in treatment, male's 11 examples, women's 19 examples; Minimum 8 years old of age, maximum 32 years old; Be chronic gingivitis, wherein simple red swelling of gingiva, kermesinus, gingival sulcus are deepened 21 examples, gingival sulcus pyorrhea 3 examples, rotten to the corn and gingiva severe haemorrhage 6 examples of gum edge.Choose 30 routine patients in addition as matched group, male's 14 examples, women's 16 examples; Minimum 9 years old of age, maximum 40 years old; Simple red swelling of gingiva, kermesinus, gingival sulcus are deepened 18 examples, gingival sulcus pyorrhea 4 examples, the rotten to the corn and 8 serious examples of gingival hemorrhage of gum edge.Whole cases persons that do not have the odontoseisis, dental calculus I degree～II degree, film making shows no alveolus bone resorption, lab testing: total white blood cells (4.0～9.0) * 10 ⁹/ L, neutrophilic granulocyte 0.7～0.8.

Therapeutic Method

Treatment group: the preparation of Chinese medicine collutory: prescription: Radix Angelicae Sinensis, Indigo Naturalis, Radix Salviae Miltiorrhizae, Cortex Lycii, Radix Notoginseng, the Radix Angelicae Dahuricae, Rhizoma Et Radix Notopterygii, Semen Arecae, Flos Lonicerae, Fructus Gardeniae, Radix Scutellariae, Cortex Phellodendri, Borneolum Syntheticum, Herba Menthae, Herba Patriniae according to the present invention.Add metronidazole and process collutory, dilute 50% subsequent use.Remove dental calculus during treatment, require the patient by the doctor's advice rinsing the mouth with the Chinese medicine collutory of preparation, every day 4 times is after meal early, middle and late, gargles each 20ml, continuous 2 weeks just before going to bed.

Matched group adopts conventional therapy, removes dental calculus, hydrogen peroxide, normal saline flushing oral cavity, and red swelling of gingiva is coated with iodine glycerol outside the place, and every day, the hibitane collutory was gargled.

Check and write down facing gingivitis indices (GI), gingival hemorrhage index (SB I), the plaque index (PL I) of two groups of patient's rinsing the mouth front and back.

Therapeutic outcome

The evaluation of gingivitis criterion of therapeutical effect: produce effects: subjective symptom is clearly better, and GI, SB I, PL I all descend; Effectively: subjective symptom takes a turn for the better, and GI, SB I, PL I have 1 decline; Invalid: subjective symptom does not have improvement GI, SB I, PL I and does not have change.

After treating half a year, follow up a case by regular visits to for two groups, in Chinese medicine collutory group 30 examples, produce effects 18 examples, effective 11 examples, invalid 1 example, effective percentage reaches 96%.Because of enamel hypoplasia, facing is coarse in invalid 1 example, and bacterial plaque is prone to adhere to.In matched group 30 examples, produce effects 14 examples, effective 12 examples, invalid 4 examples, effective percentage reaches 86%.2 routine dental fluorosis are arranged in invalid 4 examples.

Through two groups of statistical procedures significant difference (P＜0.01) is arranged relatively, the Chinese medicine collutory is described treatment gingivitis, the growth of control bacterial plaque, more remarkable than simple hibitane collutory therapeutic effect.

Discuss:

Generally acknowledge that at present gingivitis is an infectious disease, the main source of infection is the microorganism of dental plaque in neck portion and the gingival sulcus.Bacterial plaque microorganism and product long term thereof cause the immune response of body in gingiva, at first cause the inflammatory reaction of gingiva.When suffering from gingivitis, pathological changes is confined in gingival epithelium and the connective tissue.Be extended down to deep periodontal tissue when inflammation expands, can cause variations such as frontal resorption and pericemental dissolving, destruction.So control dental plaque effectively, the control gingivitis, what block periodontal disease is our primary work.Thoroughly remove tartar, smooth face meets biological requirement, makes gingival connective tissue might be attached to the root face again, forms and newly adheres to.

In the Chinese medicine collutory main component, Semen Arecae, metronidazole have stronger bactericidal action.The Semen Arecae water decoction has inhibitory action to streptococcus mutans killer cell adhesion.Cortex Phellodendri, Radix Et Rhizoma Rhei etc. to the growth of Streptococcus mutans, produce acid and sticking of hard thing certain inhibitory action arranged.Flos Lonicerae in the Chinese medicine, Herba Menthae have the effect of reducing swelling and alleviating pain.Herba Menthae can be eliminated the bitterness in the Chinese medicine, and makes people's breath freshening, and the mouthfeel when gargling is fine.Metronidazole has antibacterial activity to anaerobe, can penetrate microorganism, and destruction DNA of bacteria bacterium chain perhaps suppresses it and synthesizes.Hibitane the with a long history of hydraulic control bacterial plaque of gargling, effect is comparatively sure, but chlohexidine makes its clinical practice receive certain restriction because of having shortcomings such as the tooth of making dyeing, bitter in the mouth.Chinese herbal medicine has advantages such as the medicine source is abundant, price is low, side effect is little, so the Chinese medicine collutory has bigger clinical use prospect.

Specific embodiment 1, often certain, the man, 9 years old, various focusing depths represented, pain, seek medical advice hemorrhage January when brushing teeth, inspection sees and is aubergine by swelling and aching of gum, touches to be prone to hemorrhagely, touches a tender spot obviously.Diagnosis: marginal gingivitis.Once all there was not positive effect with multiple medicine rinsing the mouth.Use Chinese medicine preparation of the present invention: Radix Angelicae Sinensis 10g, Indigo Naturalis 15g, Radix Salviae Miltiorrhizae 15g, Cortex Lycii 10g, Radix Notoginseng 10g, Radix Angelicae Dahuricae 10g, Rhizoma Et Radix Notopterygii 10g, Semen Arecae 15g, Flos Lonicerae 10g, Fructus Gardeniae 10g, Radix Scutellariae 10g, Cortex Phellodendri 10g, Borneolum Syntheticum 10g, Herba Menthae 10g, Herba Patriniae 10g processes and sucks liquid; The metronidazole that adds dilution is processed collutory, day gargles four times, and each 10ml, rinsing the mouth was told in 3 minutes) swelling and aching of gum obviously disappears hemorrhage minimizing when brushing teeth.Take the present invention and sucked liquid 5, gingiva is not hemorrhage during conscious brushing teeth, and gingiva is pain not, and inspection sees that gingiva recovers normal.

Specific embodiment 2, Wu, the man, 34 years old, regular gingival hemorrhage was checked simple red swelling of gingiva, kermesinus, gingival sulcus intensification, forms a large amount of dental plaques.Diagnosis: simple gingivitis.Once all there was not positive effect with multiple medicine rinsing the mouth.Use Chinese medicine preparation of the present invention: Radix Angelicae Sinensis 15g, Indigo Naturalis 15g, Radix Salviae Miltiorrhizae 15g, Cortex Lycii 10g, Radix Notoginseng 15g, Radix Angelicae Dahuricae 10g, Rhizoma Et Radix Notopterygii 10g, Semen Arecae 15g, Flos Lonicerae 15g, Fructus Gardeniae 15g, Radix Scutellariae 10g, Cortex Phellodendri 10g, Borneolum Syntheticum 10g, Herba Menthae 10g, Herba Patriniae 15g processes and sucks liquid; The metronidazole that adds dilution is processed collutory, day gargles four times, and each 10ml, rinsing the mouth was told in 3 minutes) swelling and aching of gum obviously disappears hemorrhage minimizing when brushing teeth.Take the present invention and sucked liquid 5, gingiva is not hemorrhage during conscious brushing teeth, and gingiva is pain not, and dental plaque slowly disappears, and inspection sees that gingiva recovers normal.

Specific embodiment 3: seal certain, the woman, 23 years old, regular gingival swelling and pain gingival hemorrhage was checked simple red swelling of gingiva, kermesinus, gingival sulcus intensification, forms a large amount of dental plaques, dental calculus.Diagnosis: marginal gingivitis.Once all there was not positive effect with multiple medicine rinsing the mouth.Use Chinese medicine preparation of the present invention: Radix Angelicae Sinensis 10g, Indigo Naturalis 15g, Radix Salviae Miltiorrhizae 15g, Cortex Lycii 10g, Radix Notoginseng 15g, Radix Angelicae Dahuricae 15g, Rhizoma Et Radix Notopterygii 10g, Semen Arecae 15g, Flos Lonicerae 15g, Fructus Gardeniae 15g, Radix Scutellariae 10g, Cortex Phellodendri 15g, Borneolum Syntheticum 15g, Herba Menthae 10g, Herba Patriniae 15g processes and sucks liquid; The metronidazole that adds dilution is processed collutory, day gargles four times, and each 10ml, rinsing the mouth was told in 3 minutes) swelling and aching of gum obviously disappears hemorrhage minimizing when brushing teeth.Take the present invention and sucked liquid 5, gingiva is not hemorrhage during conscious brushing teeth, and gingiva is pain not, and dental plaque slowly disappears, and inspection sees that gingiva recovers normal.

Claims (9)

1. an oral cavity Chinese medicine preparation of treating gingivitis is characterized in that the ratio of weight and number of various crude drug is in the said preparation: 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Indigo Naturaliss, 10～20 parts of Radix Salviae Miltiorrhizaes; 10～20 parts of Cortex Lycii, 10～20 parts of Radix Notoginseng, 10～20 parts of the Radixs Angelicae Dahuricae, 10～20 parts of Rhizoma Et Radix Notopterygiis; 10～20 parts of Semen Arecaes, 10～20 parts of Flos Loniceraes, 10～20 parts of Fructus Gardeniaes, 10～20 parts of Radix Scutellariaes; 10～20 parts of Cortex Phellodendris, 10～20 parts of Borneolum Syntheticums, 10～20 parts of Herba Menthaes, 10～20 parts of Herba Patriniae.

2. according to the oral cavity Chinese medicine preparation of the said treatment gingivitis of claim 1, it is characterized in that the ratio of weight and number of various crude drug is in the said preparation: 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Indigo Naturaliss; 10～20 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Cortex Lycii, 10～20 parts of Radix Notoginseng, 10～20 parts of the Radixs Angelicae Dahuricae; 10～20 parts of Rhizoma Et Radix Notopterygiis, 10～15 parts of Semen Arecaes, 10～15 parts of Flos Loniceraes, 10～15 parts of Fructus Gardeniaes; 10～15 parts of Radix Scutellariaes, 10～15 parts of Cortex Phellodendris, 10～15 parts of Borneolum Syntheticums, 10～15 parts of Herba Menthaes, 10～15 parts of Herba Patriniae.

3. according to the oral cavity Chinese medicine preparation of the said treatment gingivitis of claim 1, it is characterized in that the ratio of weight and number of various crude drug is in the said preparation: 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of Indigo Naturaliss, 10～15 parts of Radix Salviae Miltiorrhizaes; 10～15 parts of Cortex Lycii, 10～15 parts of Radix Notoginseng, 10～15 parts of the Radixs Angelicae Dahuricae, 10～15 parts of Rhizoma Et Radix Notopterygiis; 10～20 parts of Semen Arecaes, 10～20 parts of Flos Loniceraes, 10～20 parts of Fructus Gardeniaes, 10～20 parts of Radix Scutellariaes; 10～20 parts of Cortex Phellodendris, 10～20 parts of Borneolum Syntheticums, 10～20 parts of Herba Menthaes, 10～20 parts of Herba Patriniae.

4. according to the oral cavity Chinese medicine preparation of each said treatment gingivitis in the claim 1～3, it is characterized in that its dosage form is: suck liquid, buccal tablet, spray, powder, collutory, slow releasing capsule, effervescent tablet or liniment.

5. method for preparing like the oral cavity Chinese medicine preparation of each said treatment gingivitis in the claim 1～4; It is characterized in that; The dosage form of said preparation is a collutory, and its preparation method may further comprise the steps: Borneolum Syntheticum, Herba Menthae and Rhizoma Et Radix Notopterygii are extracted with steam distillation, get crystal and sucking filtration liquid; Sucking filtration liquid concentrate water extract a, the crystal dissolve with ethanol gets pure liquid b; After all the other flavour of a drug mixed the back decocte with water, water extract decompression thin film concentration to relative density 1.05～1.10 was used ethanol precipitation; Merge decompression thin film concentration to relative density 1.02～1.06 after the precipitate with ethanol supernatant concentration with above-mentioned water extract a, pure liquid b; Concentrated solution adds metronidazole, thin up, and packing promptly gets collutory.

6. according to the oral cavity Chinese medicine preparation of the said treatment gingivitis of claim 5, it is characterized in that, in the said preparation process; Above-mentioned water extraction process is: decocting boils secondary, and 8～12 times of quality of medicinal material water decocted 1～3 hour for the first time; 6～10 times of quality of medicinal material water decocted 1～3 hour for the second time.

7. according to the oral cavity Chinese medicine preparation of the said treatment gingivitis of claim 5, it is characterized in that in the said preparation process, alcoholic acid volumetric concentration is 60%～80% in the above-mentioned ethanol precipitation, placed 12～24 hours.

8. according to the oral cavity Chinese medicine preparation of the said treatment gingivitis of claim 5, it is characterized in that in the said preparation process, the concentration of the metronidazole that adds in the concentrated solution is dilution 50%～60% metronidazole.

9. method for preparing like the oral cavity Chinese medicine preparation of each said treatment gingivitis in the claim 1～4; It is characterized in that; The dosage form of said preparation is that the method for preparing of liniment comprises: earlier said crude drug is ground into coarse powder except that Borneolum Syntheticum and Herba Menthae, further pulverizes, cross 120 mesh sieves; The fine powder amount that enable pass is crossed 120 mesh sieves is reaching 30%, and the fine powder of collecting through 120 mesh sieves gets fine powder a; Can not drop into multi-function extractor through the coarse powder of 120 mesh sieves again and extract twice, and add 8-10 times of water gaging for the first time and decocted 1-2 hour, and add 4-8 times of water gaging for the second time and decocted 1-2 hour, merge decocting liquid twice, and cross and filter filtrating; Heating is concentrated into pasty state, puts into 80 ℃ of oven dry of baking oven postcooling, grinds to form fine powder b, and is subsequent use after a is mixed with b, then Borneolum Syntheticum, Herba Menthae extracted with steam distillation, gets crystal c; C is added in the mixture of a and b, add 0.1%～0.5% Fluorinse and process liniment.