CN113768834A - Preparation method of centella asiatica hydrolat - Google Patents
Preparation method of centella asiatica hydrolat Download PDFInfo
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- CN113768834A CN113768834A CN202110998971.XA CN202110998971A CN113768834A CN 113768834 A CN113768834 A CN 113768834A CN 202110998971 A CN202110998971 A CN 202110998971A CN 113768834 A CN113768834 A CN 113768834A
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- centella asiatica
- herba centellae
- ultrasonic
- complex enzyme
- centella
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- 244000146462 Centella asiatica Species 0.000 title claims abstract description 67
- 235000004032 Centella asiatica Nutrition 0.000 title claims abstract description 67
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 42
- 102000004190 Enzymes Human genes 0.000 claims abstract description 42
- 229940088598 enzyme Drugs 0.000 claims abstract description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 241000167550 Centella Species 0.000 claims abstract description 21
- 108060004795 Methyltransferase Proteins 0.000 claims abstract description 17
- 108090000526 Papain Proteins 0.000 claims abstract description 16
- 239000004365 Protease Substances 0.000 claims abstract description 16
- 229940055729 papain Drugs 0.000 claims abstract description 16
- 235000019834 papain Nutrition 0.000 claims abstract description 16
- 238000002791 soaking Methods 0.000 claims abstract description 16
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 238000004140 cleaning Methods 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 239000012982 microporous membrane Substances 0.000 claims abstract description 6
- -1 pectase Proteins 0.000 claims abstract description 3
- 238000010298 pulverizing process Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 20
- 108010059820 Polygalacturonase Proteins 0.000 claims description 14
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 14
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 13
- 208000002193 Pain Diseases 0.000 abstract description 5
- 230000035876 healing Effects 0.000 abstract description 5
- 230000000052 comparative effect Effects 0.000 description 16
- 238000007654 immersion Methods 0.000 description 12
- 208000027418 Wounds and injury Diseases 0.000 description 8
- 206010052428 Wound Diseases 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000004480 active ingredient Substances 0.000 description 6
- 206010042496 Sunburn Diseases 0.000 description 5
- QCYLIQBVLZBPNK-UHFFFAOYSA-N asiaticoside A Natural products O1C(C(=O)C(C)C)=CC(C)C(C2(C(OC(C)=O)CC34C5)C)C1CC2(C)C3CCC(C1(C)C)C45CCC1OC1OCC(O)C(O)C1O QCYLIQBVLZBPNK-UHFFFAOYSA-N 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 3
- 230000000202 analgesic effect Effects 0.000 description 3
- 229940106157 cellulase Drugs 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- WYQVAPGDARQUBT-FGWHUCSPSA-N Madecassol Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O)OC[C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)[C@]12CC[C@H]([C@@H]([C@H]1C=1[C@@]([C@@]3(CC[C@H]4[C@](C)(CO)[C@@H](O)[C@H](O)C[C@]4(C)[C@H]3CC=1)C)(C)CC2)C)C)[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O WYQVAPGDARQUBT-FGWHUCSPSA-N 0.000 description 2
- BNMGUJRJUUDLHW-HCZMHFOYSA-N Madecassoside Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O)OC[C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)[C@]12CC[C@H]([C@@H]([C@H]1C=1[C@@]([C@@]3(C[C@@H](O)[C@H]4[C@](C)(CO)[C@@H](O)[C@H](O)C[C@]4(C)[C@H]3CC=1)C)(C)CC2)C)C)[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O BNMGUJRJUUDLHW-HCZMHFOYSA-N 0.000 description 2
- BNMGUJRJUUDLHW-HLUHVYOBSA-N Madecassoside Natural products C[C@@H]1CC[C@@]2(CC[C@]3(C)C(=CC[C@@H]4[C@@]5(C)C[C@@H](O)[C@H](O)[C@@](C)(CO)[C@@H]5[C@H](O)C[C@@]34C)[C@@H]2[C@H]1C)C(=O)O[C@@H]6O[C@H](CO[C@@H]7O[C@H](CO)[C@@H](O[C@@H]8O[C@H](C)[C@H](O)[C@@H](O)[C@H]8O)[C@H](O)[C@H]7O)[C@@H](O)[C@H](O)[C@H]6O BNMGUJRJUUDLHW-HLUHVYOBSA-N 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 229940011658 asiatic acid Drugs 0.000 description 2
- JXSVIVRDWWRQRT-UYDOISQJSA-N asiatic acid Chemical compound C1[C@@H](O)[C@H](O)[C@@](C)(CO)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C JXSVIVRDWWRQRT-UYDOISQJSA-N 0.000 description 2
- LBGFKBYMNRAMFC-PYSQTNCISA-N asiatic acid Natural products C[C@@H]1CC[C@@]2(CC[C@]3(C)C(=CC[C@@H]4[C@@]5(C)C[C@@H](O)[C@H](O)[C@@](C)(CO)[C@@H]5CC[C@@]34C)[C@]2(C)[C@H]1C)C(=O)O LBGFKBYMNRAMFC-PYSQTNCISA-N 0.000 description 2
- WYQVAPGDARQUBT-XCWYDTOWSA-N asiaticoside Natural products O=C(O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@H](O)[C@H](O)[C@H](O[C@H]3[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O3)[C@@H](CO)O2)O1)[C@@]12[C@@H]([C@@H](C)[C@H](C)CC1)C=1[C@](C)([C@@]3(C)[C@@H]([C@@]4(C)[C@H]([C@@](CO)(C)[C@@H](O)[C@H](O)C4)CC3)CC=1)CC2 WYQVAPGDARQUBT-XCWYDTOWSA-N 0.000 description 2
- 229940022757 asiaticoside Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- CLXOLTFMHAXJST-UHFFFAOYSA-N esculentic acid Natural products C12CC=C3C4CC(C)(C(O)=O)CCC4(C(O)=O)CCC3(C)C1(C)CCC1C2(C)CCC(O)C1(CO)C CLXOLTFMHAXJST-UHFFFAOYSA-N 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 229940011656 madecassic acid Drugs 0.000 description 2
- BUWCHLVSSFQLPN-UHFFFAOYSA-N madecassic acid Natural products CC1CCC2(CCC3(C)C(=CCC4C5(C)CC(O)C(O)C(C)(C5CCC34C)C(=O)O)C2C1C)C(=O)OC6OC(COC7OC(CO)C(OC8OC(C)C(O)C(O)C8O)C(O)C7O)C(O)C(O)C6O BUWCHLVSSFQLPN-UHFFFAOYSA-N 0.000 description 2
- PRAUVHZJPXOEIF-AOLYGAPISA-N madecassic acid Chemical compound C1[C@@H](O)[C@H](O)[C@@](C)(CO)[C@@H]2[C@H](O)C[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C PRAUVHZJPXOEIF-AOLYGAPISA-N 0.000 description 2
- 229940090813 madecassoside Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241001075517 Abelmoschus Species 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 206010006784 Burning sensation Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229960002233 benzalkonium bromide Drugs 0.000 description 1
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Alternative & Traditional Medicine (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a preparation method of centella asiatica hydrosol, which comprises the following steps: (1) cleaning fresh picked herba Centellae, and pulverizing; (2) cold soaking pulverized herba Centellae in 20-30% ethanol solution for 5-10min to obtain herba Centellae cold soaking solution; (3) adding complex enzyme into herba Centellae cold steep, wherein the complex enzyme is prepared from helicase, pectase, and papain, and performing enzymolysis at 40-50 deg.C for 20-30 min; then ultrasonic extraction is carried out for 45-55min, and the ultrasonic power is 135-145W, so as to obtain the asiatic centella ultrasonic liquid; (4) heating herba Centellae ultrasonic solution to 95-105 deg.C, treating for 8-12min, cooling, centrifuging, collecting supernatant, and filtering with microporous membrane to obtain herba Centellae hydrolat. The centella asiatica hydrosol prepared by the invention has obvious effect on post-solarization repair, and not only can rapidly relieve pain, but also can accelerate healing.
Description
Technical Field
The invention relates to the field of plant hydrolat, and particularly relates to a preparation method of centella asiatica hydrolat.
Background
Centella asiatica (L) Urb), also known as horseshoe grass, is distributed in provinces of shanxi, jiangsu, anhui, zhejiang, jiangxi, hunan, north of lake, south of the sea, fujian, taiwan, guangdong, guangxi, sichuan, Yunnan provinces of china. Has antibacterial, antiinflammatory, and analgesic effects, and can be used as food and medicinal materials. The general pure dew of centella asiatica is prepared by heating and distilling centella asiatica, the process is simple, but the effective components of the centella asiatica are easy to damage by long-time high-temperature distillation, and the prepared pure dew has low content of the effective components. The pure herba centellae syrup on the market has the effects of replenishing water, fading acne marks and the like.
The temperature is lower in winter, people prefer to take holidays at seas in summer, particularly people are cool when people play in a maritime project, the skin is inevitably exposed to the sun for a long time when people play at seas or at seas, particularly women who like bikini wear are particularly likely to suffer sunburn when the skin is exposed to the sun for a long time, the sunburn is easily caused, the burning pain feeling is obvious after sunburn, and the repairing time of serious people is long. The existing centella asiatica hydrolat has a general repairing effect after being used in the sun, can not relieve pain quickly, has long wound healing time after being used, and can not meet the requirements of people. Therefore, the invention researches a novel process for preparing the centella asiatica hydrosol, and aims to prepare the centella asiatica hydrosol to meet the requirement of quick repair after being dried in the sun.
Disclosure of Invention
In view of this, the present invention provides a method for preparing pure herba centellae distillate, which solves the above problems.
The technical scheme of the invention is realized as follows:
a preparation method of centella asiatica hydrosol comprises the following steps:
(1) cleaning fresh picked herba Centellae, and pulverizing;
(2) cold soaking pulverized herba Centellae in 20-30% ethanol solution for 5-10min to obtain herba Centellae cold soaking solution; according to the invention, ethanol solution with a certain concentration is used for cold soaking, so that the softening of asiatic pennywort herb tissues is facilitated, the dissolution of active ingredients is improved, and the subsequent enzymolysis-ultrasonic extraction is facilitated; the use of an ethanol solution with too high concentration not only increases the cost, but also is not beneficial to the subsequent removal of ethanol.
(3) Adding complex enzyme into herba Centellae cold steep, wherein the complex enzyme is prepared from helicase, pectase, and papain, and performing enzymolysis at 40-50 deg.C for 20-30 min; then ultrasonic extraction is carried out for 45-55min, and the ultrasonic power is 135-145W, so as to obtain the asiatic centella ultrasonic liquid; after the ethanol is soaked in cold water, the method adopts helicase, pectinase and papain to carry out compound enzymolysis and ultrasonic extraction, and fully extracts the effective components of asiaticoside, madecassoside, asiatic acid, madecassic acid and the like.
(4) Heating herba Centellae ultrasonic solution to 95-105 deg.C, treating for 8-12min, cooling, centrifuging, collecting supernatant, and filtering with microporous membrane to obtain herba Centellae hydrolat. The method is used for heating and inactivating enzyme in a certain temperature within a short time, simultaneously removing ethanol, avoiding the damage of active ingredients by the traditional long-time high-temperature distillation method, and effectively improving the active ingredients in the centella asiatica hydrosol.
Preferably, in the step (2), the mass-to-volume ratio g/ml of the centella asiatica to the ethanol solution is 1: 18-22.
Preferably, in the step (3), the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.5-0.8: 0.2-0.4.
Preferably, in the step (3), the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.65: 0.3, wherein the addition amount of the complex enzyme is 0.28 percent of the mass of the crushed centella asiatica.
Preferably, in the step (3), the addition amount of the complex enzyme is 0.2-0.3% of the mass of the crushed centella asiatica.
Preferably, in the step (3), the ultrasonic temperature is 43-48 ℃. More preferably, the sonication temperature is 45 ℃.
Preferably, the rotation speed of the centrifugation in the step (4) is 2500-2800rpm, and the centrifugation time is 15-20 min.
Preferably, in the step (4), the pore size of the microporous filter membrane is 0.22 μm.
Compared with the prior art, the invention has the beneficial effects that:
the method takes fresh centella asiatica as a raw material, adopts the combination of helicase, pectinase and papain for enzymolysis and ultrasonic extraction after ethanol cold soaking, fully extracts effective components such as asiaticoside, madecassoside, asiatic acid, madecassic acid and the like, and finally removes ethanol while heating and inactivating enzyme, so that the active components are prevented from being damaged by a traditional high-temperature distillation method for a long time, and the active components in the centella asiatica hydrosol are effectively improved. The centella asiatica hydrosol prepared by the invention has a remarkable effect on after-sun repair, can quickly relieve pain, accelerates healing and can meet the demand of quick after-sun repair.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A preparation method of centella asiatica hydrosol comprises the following steps:
(1) cleaning fresh picked centella, and crushing by adopting a wet crusher;
(2) placing the crushed centella into an ethanol solution with the mass concentration of 20% for cold soaking for 10min, wherein the material-liquid ratio is 1 g: 22ml, obtaining a centella asiatica cold immersion liquid;
(3) adding a complex enzyme into the centella asiatica cold immersion liquid, wherein the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.5: 0.4, adding the complex enzyme with the addition amount of 0.20 percent of the mass of the crushed centella asiatica, and performing enzymolysis at 40 ℃ for 30 min; performing ultrasonic extraction for 45min under the conditions of ultrasonic power of 145W and ultrasonic temperature of 43 ℃ to obtain an ultrasonic solution of centella asiatica;
(4) heating the herba Centellae ultrasonic solution to 95 deg.C, treating for 12min, cooling, centrifuging in a centrifuge at 2500rpm for 20min, collecting supernatant, and filtering with 0.22 μm microporous membrane to obtain herba Centellae hydrolat.
Example 2
A preparation method of centella asiatica hydrosol comprises the following steps:
(1) cleaning fresh picked centella, and crushing by adopting a wet crusher;
(2) placing the crushed centella into an ethanol solution with the mass concentration of 30% for cold soaking for 5min, wherein the material-liquid ratio is 1 g: 18ml, obtaining a centella asiatica cold immersion liquid;
(3) adding a complex enzyme into the centella asiatica cold immersion liquid, wherein the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.8: 0.2, adding the complex enzyme with the addition amount of 0.20 percent of the mass of the crushed centella asiatica, and performing enzymolysis at 50 ℃ for 20 min; performing ultrasonic extraction for 55min at ultrasonic power of 135W and ultrasonic temperature of 48 deg.C to obtain herba Centellae ultrasonic solution;
(4) heating the herba Centellae ultrasonic solution to 105 deg.C, treating for 8min, cooling, centrifuging in a centrifuge at 2800rpm for 15min, collecting supernatant, and filtering with 0.22 μm microporous membrane to obtain herba Centellae hydrolat.
Example 3
A preparation method of centella asiatica hydrosol comprises the following steps:
(1) cleaning fresh picked centella, and crushing by adopting a wet crusher;
(2) placing the crushed centella into an ethanol solution with the mass concentration of 25% for cold soaking for 8min, wherein the material-liquid ratio is 1 g: 20ml, obtaining a centella asiatica cold immersion liquid;
(3) adding complex enzyme into the centella asiatica cold immersion liquid, wherein the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1.00: 0.70: 0.35, adding complex enzyme 0.30% of the pulverized herba Centellae, and performing enzymolysis at 45 deg.C for 25 min; performing ultrasonic extraction for 50min under the conditions of ultrasonic power of 145W and ultrasonic temperature of 48 ℃ to obtain an ultrasonic solution of centella asiatica;
(4) heating the centella ultrasonic liquid to 100 ℃, treating for 10min, cooling, placing in a centrifuge, centrifuging for 18min at the centrifugal rotation speed of 2700rpm, collecting supernatant, and filtering the supernatant with a 0.22 mu m microporous filter membrane to obtain the centella hydrolat.
Example 4
A preparation method of centella asiatica hydrosol comprises the following steps:
(1) cleaning fresh picked centella, and crushing by adopting a wet crusher;
(2) placing the crushed centella into an ethanol solution with the mass concentration of 23% for cold soaking for 8min, wherein the material-liquid ratio is 1 g: 20ml, obtaining a centella asiatica cold immersion liquid;
(3) adding complex enzyme into the centella asiatica cold immersion liquid, wherein the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1.00: 0.65: 0.30, adding complex enzyme 0.28% of the pulverized herba Centellae, and performing enzymolysis at 45 deg.C for 28 min; then carrying out ultrasonic extraction for 50min under the conditions of ultrasonic power of 140W and ultrasonic temperature of 45 ℃ to obtain an asiatic pennywort herb ultrasonic liquid;
(4) heating the centella ultrasonic liquid to 100 ℃, treating for 10min, cooling, placing in a centrifuge, centrifuging for 18min at the centrifugal rotation speed of 2600rpm, collecting supernatant, and filtering the supernatant with a 0.22-micron microporous filter membrane to obtain the centella hydrolat.
Comparative example 1
Comparative example 1 is different from example 4 mainly in that the ethanol solution concentration in step (2) is 10% and the cold soaking time is 20 min. The method specifically comprises the following steps: in the step (2), the crushed centella is placed in an ethanol solution with the mass concentration of 10% for cold soaking for 20min, and the material-liquid ratio is 1 g: 20ml, and obtaining the centella asiatica cold immersion liquid.
Comparative example 2
The comparative example 2 is mainly different from the example 4 in that in the step (3), the compound enzyme is prepared from helicase, pectinase and cellulase according to the mass ratio of 1: 1: 1 to obtain the product. The method specifically comprises the following steps: in the step (3), a complex enzyme is added into the centella asiatica cold immersion liquid, and the complex enzyme is prepared from helicase, pectinase and cellulase according to the mass ratio of 1: 1: 1, adding the complex enzyme, wherein the adding amount of the complex enzyme is 0.28 percent of the mass of the crushed centella asiatica, and performing enzymolysis at 45 ℃ for 28 min; and performing ultrasonic extraction for 50min under the conditions of ultrasonic power of 140W and ultrasonic temperature of 45 ℃ to obtain the asiatic pennywort herb ultrasonic liquid.
Comparative example 3
Comparative example 3 differs from example 4 mainly in that the process conditions in step (3) are different. The method specifically comprises the following steps: in the step (3), a complex enzyme is added into the centella asiatica cold immersion liquid, and the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1.00: 0.65: 0.30, adding complex enzyme 0.28% of the pulverized herba Centellae, and performing enzymolysis at 55 deg.C for 25 min; and performing ultrasonic extraction for 50min under the conditions of ultrasonic power of 160W and ultrasonic temperature of 55 ℃ to obtain the asiatic pennywort herb ultrasonic liquid.
Comparative example 4
The main difference between the comparative example 4 and the example 4 is that the process conditions in the step (4) are different, specifically: in the step (4), the centella ultrasonic liquid is heated to 110 ℃ for 20min, the centella ultrasonic liquid is cooled and then placed in a centrifuge for centrifugation for 18min, the centrifugation speed is 2600rpm, the supernatant is collected and filtered by a 0.22 mu m microporous filter membrane, and the centella hydrolat is prepared.
Comparative example 5
The comparative example 5 is different from the example 4 mainly in the proportion and the dosage of the complex enzyme in the step (3). The method specifically comprises the following steps: in the step (3), a complex enzyme is added into the centella asiatica cold immersion liquid, and the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 1: 1, adding the complex enzyme, wherein the adding amount of the complex enzyme is 0.35 percent of the mass of the crushed centella asiatica, and performing enzymolysis at 45 ℃ for 28 min; and performing ultrasonic extraction for 50min under the conditions of ultrasonic power of 140W and ultrasonic temperature of 45 ℃ to obtain the asiatic pennywort herb ultrasonic liquid.
Test example-post-basking repair test
(1) General data: 130 volunteers, all female, aged 18-36 years, averaged 26.3 years, were collected and randomized into trial 1-9 and control groups of 13 each. The time from sunburn to treatment is 12-36h, and the average time is 18 h. The skin of the sunburn is red and swollen, has obvious burning sensation, and the exposed parts of the face, the neck, the upper limbs and the like have erythema, pimple or herpes dunghill, and the average skin damage area is 8.7 percent. The test groups 1 to 4 used the centella asiatica hydrolat prepared in examples 1 to 4 in sequence, and the test groups 5 to 9 used the centella asiatica hydrolat prepared in comparative examples 1 to 5 in sequence; the control group was sprayed with commercially available Abelmoschus trifoliatus hydrolat.
(2) The treatment method comprises the following steps: cleaning the wound surface with normal saline, sterilizing the obviously polluted wound surface with 1% benzalkonium bromide solution, directly and uniformly spraying herba Centellae hydrolat the wound surface after the sterile gauze is dipped in the dry wound surface, administrating once every 5 hours, and removing the wound surface exudate with a cotton swab before spraying to ensure the clean and moist wound surface. The wound surface is not painful or bleeding due to the principle of no injury in the treatment process. The control group was prescribed in accordance with the test group. The treatment course is 7 days.
(3) As a result: the analgesic time and healing time of each test group and control group were counted separately, and the initial time was calculated by using the first time centella asiatica hydrolat, and the results are shown in table 1 below.
P <0.05, p <0.01, compared to control group
The results show that compared with the commercially available centella pure dew, each test group has certain effect on after-sun repair, and can not only rapidly relieve pain, but also accelerate healing. Of these, examples 1 to 4 are particularly effective in greatly shortening the analgesic time and the healing time.
The ethanol solution used in the comparative example 1 has a low concentration, so that even if the cold soaking time is prolonged, the cold soaking effect is also obviously reduced, the subsequent extraction of active ingredients is not facilitated, and the repairing effect of the centella asiatica after being exposed to the sun is obviously reduced.
Comparative example 2 adopts cellulase to replace papain, the enzymolysis effect is reduced, and the repairing effect of the centella asiatica after being exposed to the sun is greatly reduced. Comparative example 5 adopts an equivalent enzyme ratio, and the repairing effect of the centella asiatica after being exposed to the sun is also reduced.
Comparative example 3 does not adopt the preferred ultrasonic extraction conditions of the invention, increases the proportion of the damaged active ingredients, reduces the extraction effect, and obviously reduces the repairing effect of the centella after being exposed to the sun.
Comparative example 4 the heat treatment temperature was increased to destroy more active ingredients and the effect of repairing centella asiatica after exposure to the sun was also significantly reduced.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. The preparation method of the centella asiatica hydrosol is characterized by comprising the following steps:
(1) cleaning fresh picked herba Centellae, and pulverizing;
(2) cold soaking pulverized herba Centellae in 20-30% ethanol solution for 5-10min to obtain herba Centellae cold soaking solution;
(3) adding complex enzyme into herba Centellae cold steep, wherein the complex enzyme is prepared from helicase, pectase, and papain, and performing enzymolysis at 40-50 deg.C for 20-30 min; then ultrasonic extraction is carried out for 45-55min, and the ultrasonic power is 135-145W, so as to obtain the asiatic centella ultrasonic liquid;
(4) heating herba Centellae ultrasonic solution to 95-105 deg.C, treating for 8-12min, cooling, centrifuging, collecting supernatant, and filtering with microporous membrane to obtain herba Centellae hydrolat.
2. The method for preparing centella asiatica hydrosol according to claim 1, wherein in step (2), the mass-to-volume ratio g/ml of centella asiatica to ethanol solution is 1: 18-22.
3. The preparation method of centella asiatica hydrosol according to claim 1 or 2, wherein in the step (3), the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.5-0.8: 0.2-0.4.
4. The preparation method of centella asiatica hydrosol according to claim 3, wherein in the step (3), the addition amount of the complex enzyme is 0.2% -0.3% of the mass of the crushed centella asiatica.
5. The method for preparing centella asiatica dew according to claim 1, wherein the ultrasonic temperature in step (3) is 43-48 ℃.
6. The preparation method of centella asiatica hydrosol according to claim 4, wherein in the step (3), the complex enzyme is prepared from helicase, pectinase and papain in a mass ratio of 1: 0.65: 0.3.
7. The preparation method of centella asiatica hydrosol according to claim 6, wherein in step (3), the amount of the complex enzyme is 0.28% of the mass of the crushed centella asiatica.
8. The method for preparing centella asiatica dew according to claim 5, wherein the ultrasonic temperature in step (3) is 45 ℃.
9. The method for preparing centella asiatica dew according to claim 1 or 5, wherein the centrifugation speed in step (4) is 2500-2800rpm, and the centrifugation time is 15-20 min.
10. The method for preparing centella asiatica dew according to claim 1, wherein in step (4), said microporous membrane has a pore size of 0.22 μm.
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| CN104072569A (en) * | 2014-06-16 | 2014-10-01 | 南京泽朗医药科技有限公司 | Method for extracting asiaticoside from centella |
| CN106511142A (en) * | 2017-01-18 | 2017-03-22 | 广西南宁博智生物科技有限公司 | Centella asiatica floral water and preparation method and application thereof |
| CN113171321A (en) * | 2021-05-11 | 2021-07-27 | 清远市望莎生物科技有限公司 | Centella asiatica extract and application thereof in preparation of anti-inflammatory cosmetics |
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| CN104072569A (en) * | 2014-06-16 | 2014-10-01 | 南京泽朗医药科技有限公司 | Method for extracting asiaticoside from centella |
| CN106511142A (en) * | 2017-01-18 | 2017-03-22 | 广西南宁博智生物科技有限公司 | Centella asiatica floral water and preparation method and application thereof |
| CN113171321A (en) * | 2021-05-11 | 2021-07-27 | 清远市望莎生物科技有限公司 | Centella asiatica extract and application thereof in preparation of anti-inflammatory cosmetics |
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| CN115624514A (en) * | 2022-10-12 | 2023-01-20 | 杭州时光肌生物科技有限公司 | Preparation method of composite plant extract for improving facial redness |
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