CN102908383B - Method for preparing antivenom oral liquid - Google Patents
Method for preparing antivenom oral liquid Download PDFInfo
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- CN102908383B CN102908383B CN201210449444.4A CN201210449444A CN102908383B CN 102908383 B CN102908383 B CN 102908383B CN 201210449444 A CN201210449444 A CN 201210449444A CN 102908383 B CN102908383 B CN 102908383B
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- oral liquid
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Abstract
The invention discloses a method for preparing an antivenom oral liquid with tripterospermum cordatum as the major raw material. The method comprises the following steps of: (1) treatment of the tripterospermum cordatum raw medicinal material: performing vacuum drying, crushing and enzymolysis on the raw medicinal material; (2) extraction of active ingredients of tripterospermum cordatum: performing twice ultrasonic extraction with water as a solvent, blending the filtrates and then performing alcohol precipitation to remove impurities; and (3) preparation of the antivenom oral liquid: after freezing and filtering, filtering the mixed filtrate by using a bacterial filter in a sterile area and then filling the filtered liquid. The antivenom oral liquid obtained by the method disclosed by the invention has tremendous curative effect on neural venoms such as the snake venom of coral snake and the like, and blood circulation venoms and mixed venoms and snake wounds caused by long-noded pit viper, cobra and the like; and the effect of the antivenom oral liquid is superior to that of the traditional medication ways such as taking orally after decocting with water and dressing the wound after chewing. The method is convenient for industrialized, large-scale and standardized production, and wide in market prospect.
Description
Technical field
The present invention relates to a kind of preparation method of venom oral liquid, specifically refer to take the method that high eyebrow Tripterospermum affine is main material production venom oral liquid.
Background technology
Mount emei Tripterospermum Gentianaceae Tripterospermum, latin name
tripterospermum cordatum (Marq.) H. Smith, be perennial evergreen voluble herb plant, be a kind of more conventional Chinese herbal medicine, be mainly distributed in the areas such as Hunan, Hubei, Chongqing, Sichuan, Guizhou, Yunnan.The traditional Chinese medical science records that it is cold in nature, sweet in the mouth is pungent, has the functions such as lung moistening hemostasis, clearing away heat and promoting diuresis, removing toxic substances, detumescence.
Among the people the having of China utilizes high eyebrow Tripterospermum affine as the way of anti-venom drug, and Main is oral after decocting or chews rear deposited wound have certain curative effect, but not fully up to expectations.Therefore, the high eyebrow Tripterospermum affine of take develops as primary raw material the economic worth that eutherapeutic anti-venom drug can promote high eyebrow Tripterospermum affine, makes rational use of resources, promotes the well-being of mankind.
Summary of the invention
For developing high eyebrow Tripterospermum affine resource, promoting the well-being of mankind, promote the venom effect of high eyebrow Tripterospermum affine, the inventor is through lot of experiments, finally invent a kind of method that high eyebrow Tripterospermum affine is main material production venom oral liquid of take, the venom oral liquid treatment snake venom that uses the method to produce, good effect, cure rate are high.
The present invention includes following step:
(1) processing of high eyebrow Tripterospermum affine raw medicinal material, comprises raw medicinal material is carried out to vacuum drying, pulverizing and enzymolysis.By raw medicinal material vacuum drying to moisture content, be 5%-8%, baking temperature 50-80 ℃, be crushed to again 20-40 order, enzymolysis solution enzymolysis 3-8 hour (100 grams of dry medical materials add 200-500 milliliter enzymolysis solution) at the temperature of 40-55 ℃ that then adds dry medical material 2-5 doubly to measure.Enzymolysis solution preparation method is: the 1-5 of a Jiang Meihuowei 15000-40000 unit gram cellulase is dissolved in 500ml distilled water and obtains that (international zymetology meeting regulation in 1961 is deferred to by Mei Huo unit, refer under given conditions, in 1 minute, can transform the enzyme amount of 1 micromole's substrate, or the enzyme amount of 1 micromolar relevant group in conversion of substrate).
(2) extraction of high eyebrow Tripterospermum affine effective ingredient.Above-mentioned enzymolysis solution and medical material are warming up to 80-95 ℃, extract 20-60 minute, filter and collect filtrate; In filtering residue, continue to add dry medical material 2-5 drinking water (100 grams of dry medical materials add 200-500 milliliter drinking water) doubly, be warming up to 80-95 ℃, extract 20-60 minute, filter and collect filtrate; Merge the filtrate obtaining for twice, discard medicinal residues.In extracted twice process, equal auxiliary ultrasonic, utilizes hyperacoustic cavitation effect to improve the extraction ratio of high eyebrow Tripterospermum affine effective ingredient.
1-2 times (100 grams of dry medical material distilling under reduced pressure are to 100-200 milliliter) that the filtrate decompression obtaining is distilled to dry medical material, adding filtrate equal-volume and concentration is the ethanol of 60-99.7%, standing 5-20h filters, discards filtering residue and collects filtrate.The filtrate decompression of collecting is distilled to without alcohol taste, in filtrate, add this filtrate volume 2-3 dehydrated alcohol doubly again, standing 5-20h, filter, discard filtering residue and collect filtrate, the unit that filtrate is concentrated into the 1/4-1/6(filtrate of dry medical material weight is milliliter, the unit of dry medical material weight is gram), standby after filtering.
(3) preparation of venom oral liquid.After the filtrate obtaining is cooling, put into after the standing 60-90h of refrigerator, filter, collect filtrate for later use.By after bacterial filter sterilizing, in aseptic area, with bacterial filter, filtrate is filtered, obtained venom oral liquid after fill.
The venom oral liquid that utilizes the present invention to obtain, through function test, confirm, for neural poison such as Agkistrodon (latter 3 hours innerlich anwendens bite), it is main snake venom, the blood circulation such as Agkistrodon, Naja poison and admixture poison snakebite have extraordinary curative effect, and that its effect is better than is oral after traditional decocting, chew the modes of being used as medicine such as rear deposited wound.The method is also convenient to carry out industrialization, scale and standardized production, and market prospect is wide.
Feature of the present invention is to utilize cellulase to destroy plant cell wall and hyperacoustic cavitation effect, strengthens the extraction of high eyebrow Tripterospermum affine water soluble ingredient, and the venom active constituent content extracting is improved; Later stage adopts the method for bacterial filter sterilizing to avoid conventional high temperature, the destruction of autoclaving to venom effective ingredient, retain to greatest extent the venom effective ingredient in oral liquid, made venom oral liquid curative effect that the present invention obtains be better than the general high eyebrow Tripterospermum affine mode of being used as medicine.
the specific embodiment
Below the concrete preparation method of venom oral liquid is elaborated.
Embodiment 1:
(1) processing of high eyebrow Tripterospermum affine raw medicinal material, comprises raw medicinal material is carried out to vacuum drying, pulverizing and enzymolysis.By raw medicinal material vacuum drying to moisture content, be 5.8%, 55 ℃ of baking temperatures, then be crushed to 25 orders, the enzymolysis solution that then adds 2.5 times of amounts of dry medical material enzymolysis 4 hours at the temperature of 45 ℃.Enzymolysis solution preparation method is: 2 grams of cellulase of Jiang Meihuowei 20000 units are dissolved in 500ml distilled water and get final product.
(2) extraction of high eyebrow Tripterospermum affine effective ingredient.Above-mentioned enzymolysis solution and medical material are warming up to 85 ℃, extract 30 minutes, filter and collect filtrate; In filtering residue, continue to add the drinking water of 2.2 times of dry medical materials, be warming up to 87 ℃, extract 25 minutes, filter and collect filtrate; Merge the filtrate obtaining for twice, discard medicinal residues.Equal auxiliary ultrasonic in extracted twice process.
The filtrate decompression obtaining is distilled to 1.2 times of dry medical material, adding filtrate equal-volume and concentration is 75% ethanol, and standing 8h, filters, discards filtering residue and collect filtrate.The filtrate decompression of collecting is distilled to without alcohol taste, then toward the dehydrated alcohol that adds 2.2 times of this filtrate volumes in filtrate, standing 6h, filters, discards filtering residue and collect filtrate, and filtrate is concentrated into 1/4 of dry medical material weight, standby after filtering.
(3) preparation of venom oral liquid.After the filtrate obtaining is cooling, put into after the standing 65h of refrigerator, filter, collect filtrate for later use.By after bacterial filter sterilizing, in aseptic area with bacterial filter to filtrate filter, after fill venom oral liquid.
Embodiment 2
(1) processing of high eyebrow Tripterospermum affine raw medicinal material, comprises raw medicinal material is carried out to vacuum drying, pulverizing and enzymolysis.By raw medicinal material vacuum drying to moisture content, be 7.4%, 75 ℃ of baking temperatures, then be crushed to 30 orders, the enzymolysis solution that then adds 4 times of amounts of dry medical material enzymolysis 6 hours at the temperature of 50 ℃.Enzymolysis solution preparation method is: 4 grams of cellulase of Jiang Meihuowei 30000 units are dissolved in 500ml distilled water and get final product.
(2) extraction of high eyebrow Tripterospermum affine effective ingredient.Above-mentioned enzymolysis solution and medical material are warming up to 90 ℃, extract 50 minutes, filter and collect filtrate; In filtering residue, continue to add the drinking water of 4 times of dry medical materials, be warming up to 92 ℃, extract 45 minutes, filter and collect filtrate; Merge the filtrate obtaining for twice, discard medicinal residues.Equal auxiliary ultrasonic in extracted twice process.
The filtrate decompression obtaining is distilled to 1.7 times of dry medical material, adding filtrate equal-volume and concentration is 95% ethanol, and standing 15h, filters, discards filtering residue and collect filtrate.The filtrate decompression of collecting is distilled to without alcohol taste, then toward the dehydrated alcohol that adds 2.7 times of this filtrate volumes in filtrate, standing 16h, filters, discards filtering residue and collect filtrate, and filtrate is concentrated into 1/5 of dry medical material weight, standby after filtering.
(3) preparation of venom oral liquid.After the filtrate obtaining is cooling, put into after the standing 85h of refrigerator, filter, collect filtrate for later use.By after bacterial filter sterilizing, in aseptic area with bacterial filter to filtrate filter, after fill venom oral liquid.
Claims (1)
1. the high eyebrow Tripterospermum affine of take is prepared the method for venom oral liquid as primary raw material, it is characterized in that comprising following step:
(1) processing of high eyebrow Tripterospermum affine raw medicinal material, comprises raw medicinal material is carried out to vacuum drying, pulverizing and enzymolysis; By raw medicinal material vacuum drying to moisture content, be 5%-8%, baking temperature 50-80 ℃, be crushed to again 20-40 order, then the enzymolysis solution enzymolysis 3-8 hour at the temperature of 40-55 ℃ that adds dry medical material 2-5 doubly to measure, enzymolysis solution preparation method is: the 1-5 of a Jiang Meihuowei 15000-40000 unit gram cellulase is dissolved in 500ml distilled water and get final product;
(2) extraction of high eyebrow Tripterospermum affine effective ingredient, is warming up to 80-95 ℃ by above-mentioned enzymolysis solution and medical material, extracts 20-60 minute, filters and collect filtrate; In filtering residue, continue to add dry medical material 2-5 drinking water doubly, be warming up to 80-95 ℃, extract 20-60 minute, filter and collect filtrate; Merge the filtrate obtaining for twice, discard medicinal residues; The equal auxiliary ultrasonic of extracted twice process;
The 1-2 that the filtrate decompression obtaining is distilled to dry medical material doubly, adding filtrate equal-volume and concentration is the ethanol of 60-99.7%, standing 5-20h, filter, discard filtering residue and collect filtrate, the filtrate decompression of collecting is distilled to without alcohol taste, then add this filtrate volume 2-3 dehydrated alcohol doubly in filtrate, standing 5-20h, filter, discard filtering residue and collect filtrate, filtrate is concentrated into the 1/4-1/6 of dry medical material weight, standby after filtering;
(3) preparation of venom oral liquid, puts into after the standing 60-90h of refrigerator after the filtrate obtaining is cooling, filters, and collects filtrate for later use, by after bacterial filter sterilizing, in aseptic area, with bacterial filter, filtrate is filtered, is obtained venom oral liquid after fill.
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| CN201210449444.4A CN102908383B (en) | 2012-11-12 | 2012-11-12 | Method for preparing antivenom oral liquid |
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| CN201210449444.4A CN102908383B (en) | 2012-11-12 | 2012-11-12 | Method for preparing antivenom oral liquid |
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| CN102908383B true CN102908383B (en) | 2014-04-09 |
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| CN103142684B (en) * | 2013-03-27 | 2014-07-23 | 吉首大学 | Tripterospermum cordatum extractive and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248758A (en) * | 2008-03-07 | 2008-08-27 | 吉首大学 | Tissue culture method for fine stalk double butterflies |
| KR20100041210A (en) * | 2008-10-13 | 2010-04-22 | 대한민국(관리부서 : 산림청 국립산림과학원장) | Propagation method of tripterospermum japonicum |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101248758A (en) * | 2008-03-07 | 2008-08-27 | 吉首大学 | Tissue culture method for fine stalk double butterflies |
| KR20100041210A (en) * | 2008-10-13 | 2010-04-22 | 대한민국(관리부서 : 산림청 국립산림과학원장) | Propagation method of tripterospermum japonicum |
Non-Patent Citations (6)
| Title |
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| RP-HPLC法测定峨嵋双蝴蝶中齐墩果酸的含量;敖琼等;《中国医药导刊》;20120131;第14卷(第1期);165-166 * |
| 中医药治疗毒蛇咬伤近况;周祖文等;《蛇志》;19911231;第3卷(第4期);46-49 * |
| 周祖文等.中医药治疗毒蛇咬伤近况.《蛇志》.1991,第3卷(第4期), |
| 峨嵋双蝴蝶的质量标准研究;黄冲等;《中国医药指南》;20110430;38-39 * |
| 敖琼等.RP-HPLC法测定峨嵋双蝴蝶中齐墩果酸的含量.《中国医药导刊》.2012,第14卷(第1期), |
| 黄冲等.峨嵋双蝴蝶的质量标准研究.《中国医药指南》.2011, |
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Granted publication date: 20140409 Termination date: 20171112 |