CN112089737A - Preparation technology of sarcandra glabra extract with throat clearing function - Google Patents
Preparation technology of sarcandra glabra extract with throat clearing function Download PDFInfo
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- CN112089737A CN112089737A CN202011136325.4A CN202011136325A CN112089737A CN 112089737 A CN112089737 A CN 112089737A CN 202011136325 A CN202011136325 A CN 202011136325A CN 112089737 A CN112089737 A CN 112089737A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 18
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- 238000000354 decomposition reaction Methods 0.000 claims abstract description 38
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- 230000008014 freezing Effects 0.000 claims abstract description 31
- 238000001816 cooling Methods 0.000 claims abstract description 29
- 238000000605 extraction Methods 0.000 claims abstract description 28
- 238000003892 spreading Methods 0.000 claims abstract description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
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- 238000010438 heat treatment Methods 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 24
- 229910052573 porcelain Inorganic materials 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 18
- 238000002791 soaking Methods 0.000 claims description 18
- 239000002904 solvent Substances 0.000 claims description 18
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- 239000000843 powder Substances 0.000 claims description 16
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- 241000681946 Rhododendron dauricum Species 0.000 description 1
- 240000006028 Sambucus nigra Species 0.000 description 1
- 235000003142 Sambucus nigra Nutrition 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
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- 150000002148 esters Chemical class 0.000 description 1
- 235000008995 european elder Nutrition 0.000 description 1
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- 235000011949 flavones Nutrition 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
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- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
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- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
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- 235000018553 tannin Nutrition 0.000 description 1
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- 239000001648 tannin Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- 230000008736 traumatic injury Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/04—Drugs for disorders of the respiratory system for throat disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Engineering & Computer Science (AREA)
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Abstract
The invention relates to the technical field of sarcandra glabra processing, in particular to a preparation technology of a sarcandra glabra extract with a function of clearing heat from throat, which comprises the following steps: cleaning, self-decomposition treatment of sarcandra glabra, spreading and cooling the sarcandra glabra, freezing, crushing and extracting. According to the invention, high-temperature hypoxia is used in the sarcandra glabra autolysis, so that sarcandra glabra cells are forced to degrade monosaccharides per se at a lower efficiency in an extreme environment, the thickening of sugar in subsequent crushing extraction is avoided, the processing efficiency is reduced, and the metabolism of the cells is utilized to improve the species of active ingredients. The sarcandra glabra is completely crushed by low-temperature treatment, and the prepared extract has good activity and high yield by comprehensively extracting the sarcandra glabra at high and low temperatures in combination with acid, alcohol and water.
Description
Technical Field
The invention relates to the technical field of sarcandra glabra processing, in particular to a preparation technology of a sarcandra glabra extract with a function of clearing heat from throat.
Background
Sarcandra glabra, school name: (Sarcandra glabra (Thunb.) Nakai) is perennial evergreen shrub of Chloranthaceae, commonly known as Rhododendron dauricum, herba Pileae Scriptae, Sambucus nigra, etc. It has beautiful shape, fragrance of the four seasons and extremely high medicinal, edible and ornamental values. The sarcandra glabra whole plant is used for medicine, and has the functions of clearing away heat and toxic material, dispelling wind, promoting blood circulation, eliminating swelling, relieving pain, resisting bacteria and diminishing inflammation. Can be used for treating influenza, Japanese encephalitis, pneumonia, appendicitis, pelvic inflammatory disease, traumatic injury, rheumatic arthralgia, amenorrhea, wound infection, and bacillary dysentery. It can also be used for treating malignant tumors such as pancreatic cancer, gastric cancer, rectal cancer, hepatocarcinoma, and esophageal cancer, and has effects of relieving, reducing lump, prolonging life, and improving subjective symptom without side effect. The herba Pileae Scriptae contains volatile oil, esters, phenols, tannin, flavone, cyanogenic glycoside, coumarin, lactone, etc.; the whole plant contains L-myrrhamnetin A, isofraxidin, fumaric acid, succinic acid, flavonoid glycoside and coumarins derivative. The sarcandra glabra is widely distributed and rich in resources, and is a new food raw material with great development potential.
The extract of the sarcandra glabra prepared by the prior art is mostly dissolved by high temperature, which is easy to cause the decomposition of the nutrient components in the sarcandra glabra, or the supercritical extraction technology is used, so the extraction cost is higher, and the large-scale production is not facilitated.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a preparation technology of a sarcandra glabra extract with a throat clearing function, which is used for fully protecting the functional components of sarcandra glabra and reducing the loss caused in the extraction process, and the specific technical scheme is as follows:
a preparation technology of sarcandra glabra extract with the function of clearing heat from throat comprises the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 20-30r/min for 15-20min, taking out, and draining;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 3-5cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 60-65 ℃, and preserving heat for 5-8 hours;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 40-45 deg.C, adjusting air humidity to 45-55%, and spreading for cooling for 30-50 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 20-30 min; then raising the temperature to 48-52 ℃, turning over sarcandra glabra, spraying decomposition liquid, and spreading for cooling for 4-7 h;
the preparation method of the decomposition liquid comprises the following steps: mixing and pulping 10-20 parts of hawthorn, 5-8 parts of fructus choerospondiatis and 8-12 parts of lemon in parts by mass, carrying out colloid milling on the pulp for 2-3 times, diluting by 50-100 times, soaking at 50-55 ℃ for 20-30min, filtering with 300-mesh 400-mesh filter cloth while the pulp is hot, and adding 1-3% of cellulase in the filtrate when the temperature of the filtrate is reduced to room temperature;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 20-30h, taking out the sarcandra glabra, heating to the temperature of 5-8 ℃ by using microwave, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 10-15 h;
(5) pulverizing
Putting the sarcandra glabra with ice into a crusher, crushing the sarcandra glabra into 80-100 meshes of powder at the temperature of 1-3 ℃, then raising the temperature of the powder to 20-25 ℃, and carrying out ultrasonic treatment for 30-50min by using 500-800W;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 50-55 ℃ for 20-30min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 100-200r/min for 30-50 min; then filtering with 300-400-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with clear water 10-15 times of the mass of the filter residue, heating to 80-90 ℃, stirring for 20-30min, filtering with 300-400 mesh filter cloth while hot, mixing with the previous filtrate when the temperature of the filtrate is reduced to room temperature, and concentrating and drying under reduced pressure to obtain the extract.
Further, in the step (1), the mass ratio of the sarcandra glabra to the clear water is 1: 8-10.
Further, in the step (2), the water content of the cotton cloth is 65-75%, and the temperature is 35-38 ℃.
Further, in the step (3), the amount of the decomposition liquid is 8-12% of the mass of the sarcandra glabra.
Further, in the step (3), the hawthorn is hawthorn which is close to the mature period but is not mature.
Further, in the step (3), the water content of the fructus choerospondiatis is 75-85%.
Further, in the step (4), the cooling speed is 3 ℃/min.
Further, in the step (6), the mass ratio of the mixture to the extraction solvent is 1: 3-5.
Further, in the step (4), the extraction solvent is formed by mixing ethanol and acetic acid in a mass ratio of 1: 0.5-0.8.
Compared with the prior art, the invention has the technical effects that:
according to the invention, high-temperature hypoxia is used in the sarcandra glabra autolysis, so that sarcandra glabra cells are forced to degrade monosaccharides per se at a lower efficiency in an extreme environment, the thickening of sugar in subsequent crushing extraction is avoided, the processing efficiency is reduced, and the metabolism of the cells is utilized to improve the species of active ingredients. The sarcandra glabra is completely crushed by low-temperature treatment, and the prepared extract has good activity and high yield by comprehensively extracting the sarcandra glabra at high and low temperatures in combination with acid, alcohol and water.
Detailed Description
The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.
Example 1
A preparation technology of sarcandra glabra extract with the function of clearing heat from throat comprises the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 20r/min for 15min, taking out, and draining; the mass ratio of the sarcandra glabra to the clear water is 1: 8;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 3cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 60 ℃, and preserving heat for 5 hours;
the water content of the cotton cloth is 65%, and the temperature is 35 ℃;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 40 deg.C, adjusting air humidity to 45%, and spreading for cooling for 30 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 20 min; then raising the temperature to 48 ℃, turning over the sarcandra glabra, spraying the decomposition liquid, and spreading for cooling for 4 hours;
the preparation method of the decomposition liquid comprises the following steps: mixing 10 parts of hawthorn, 5 parts of fructus choerospondiatis and 8 parts of lemon by mass, pulping, colloid-milling the pulp for 2 times, diluting by 50 times, soaking at 50 ℃ for 20min, filtering with 300-mesh filter cloth while hot, and adding 1% of cellulase into the filtrate when the temperature of the filtrate is reduced to room temperature; the using amount of the decomposition liquid is 8 percent of the mass of the sarcandra glabra; the hawthorn is close to the mature period but not mature; the water content of the fructus choerospondiatis is 75%;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 20 hours, taking out the sarcandra glabra, heating the sarcandra glabra to the temperature of 5 ℃ by using microwaves, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 10 hours;
the cooling speed is 3 ℃/min;
(5) pulverizing
Putting the sarcandra glabra with ice into a grinder, grinding the sarcandra glabra into 80-mesh powder at 1 ℃, then raising the temperature of the powder to 20 ℃, and treating the powder for 30min by using 500W ultrasonic waves;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 50 ℃ for 20min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 100r/min for 30 min; then filtering with 300-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with 10 times of clear water, heating to 80 deg.C, stirring for 20min, filtering with 300 mesh filter cloth while hot, cooling the filtrate to room temperature, mixing with the previous filtrate, concentrating under reduced pressure, and drying to obtain the extract.
The mass ratio of the mixture to the extraction solvent is 1: 3; the extraction solvent is formed by mixing ethanol and acetic acid according to the mass ratio of 1: 0.5.
Example 2
A preparation technology of sarcandra glabra extract with the function of clearing heat from throat comprises the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 30r/min for 20min, taking out, and draining; the mass ratio of the sarcandra glabra to the clear water is 1: 10;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 5cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 65 ℃, and preserving heat for 8 hours;
the water content of the cotton cloth is 75%, and the temperature is 38 ℃;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 45 deg.C, adjusting air humidity to 55%, and spreading for cooling for 50 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 30 min; then raising the temperature to 52 ℃, turning over sarcandra glabra, spraying decomposition liquid, and spreading for cooling for 7 hours;
the preparation method of the decomposition liquid comprises the following steps: mixing and pulping 20 parts of hawthorn, 8 parts of fructus choerospondiatis and 12 parts of lemon in parts by mass, carrying out colloid milling on the pulp for 3 times, diluting the pulp by 100 times, soaking the pulp for 30min at 55 ℃, filtering the pulp with 400-mesh filter cloth while the pulp is hot, and adding cellulase with the mass percent of the pulp into the filtrate when the temperature of the filtrate is reduced to room temperature; the using amount of the decomposition liquid is 12 percent of the mass of the sarcandra glabra; the hawthorn is close to the mature period but not mature; the water content of the fructus choerospondiatis is 85%;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 30 hours, taking out the sarcandra glabra, heating the sarcandra glabra to the temperature of 8 ℃ by using microwaves, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 15 hours;
the cooling speed is 3 ℃/min;
(5) pulverizing
Putting the sarcandra glabra with ice into a grinder, grinding the sarcandra glabra into 100-mesh powder at the temperature of 3 ℃, then raising the temperature of the powder to 25 ℃, and treating the powder for 50min by using 800W ultrasonic waves;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 55 ℃ for 30min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 200r/min for 50 min; then filtering with 400-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with clear water 15 times the weight of the filter residue, heating to 90 deg.C, stirring for 30min, filtering with 400 mesh filter cloth while hot, cooling the filtrate to room temperature, mixing with the previous filtrate, concentrating under reduced pressure, and drying to obtain the extract.
The mass ratio of the mixture to the extraction solvent is 1: 5; the extraction solvent is formed by mixing ethanol and acetic acid according to the mass ratio of 1: 0.8.
Example 3
A preparation technology of sarcandra glabra extract with the function of clearing heat from throat comprises the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 30r/min for 15min, taking out, and draining; the mass ratio of the sarcandra glabra to the clear water is 1: 10;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 5cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 60 ℃, and preserving heat for 8 hours;
the water content of the cotton cloth is 75%, and the temperature is 35 ℃;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 45 deg.C, adjusting air humidity to 45%, and spreading for cooling for 50 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 30 min; then raising the temperature to 48 ℃, turning over the sarcandra glabra, spraying the decomposition liquid, and spreading for cooling for 7 hours;
the preparation method of the decomposition liquid comprises the following steps: mixing and pulping 20 parts of hawthorn, 5 parts of fructus choerospondiatis and 12 parts of lemon in parts by mass, carrying out colloid milling on the pulp for 2 times, diluting the pulp by 100 times, soaking the pulp for 30min at 50 ℃, filtering the pulp with 300-mesh filter cloth while the pulp is hot, and adding cellulase with the mass percent of the pulp into the filtrate when the temperature of the filtrate is reduced to room temperature; the using amount of the decomposition liquid is 8 percent of the mass of the sarcandra glabra; the hawthorn is close to the mature period but not mature; the water content of the fructus choerospondiatis is 85%;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 30 hours, taking out the sarcandra glabra, heating the sarcandra glabra to the temperature of 5 ℃ by using microwaves, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 15 hours;
the cooling speed is 3 ℃/min;
(5) pulverizing
Putting the sarcandra glabra with ice into a grinder, grinding the sarcandra glabra into 80-mesh powder at the temperature of 3 ℃, then raising the temperature of the powder to 25 ℃, and treating the powder for 30min by using 800W ultrasonic waves;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 55 ℃ for 20min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 200r/min for 50 min; then filtering with 300-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with 15 times of clear water, heating to 80 deg.C, stirring for 30min, filtering with 400 mesh filter cloth while hot, cooling the filtrate to room temperature, mixing with the previous filtrate, concentrating under reduced pressure, and drying to obtain the extract.
The mass ratio of the mixture to the extraction solvent is 1: 5; the extraction solvent is formed by mixing ethanol and acetic acid according to the mass ratio of 1: 0.5.
Example 4
A preparation technology of sarcandra glabra extract with the function of clearing heat from throat comprises the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 24r/min for 18min, taking out, and draining; the mass ratio of the sarcandra glabra to the clear water is 1: 9;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 5cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 60 ℃, and preserving heat for 8 hours;
the water content of the cotton cloth is 75%, and the temperature is 35 ℃;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 45 deg.C, adjusting air humidity to 45%, and spreading for cooling for 50 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 30 min; then raising the temperature to 48 ℃, turning over the sarcandra glabra, spraying the decomposition liquid, and spreading for cooling for 7 hours;
the preparation method of the decomposition liquid comprises the following steps: mixing and pulping 20 parts of hawthorn, 5 parts of fructus choerospondiatis and 12 parts of lemon in parts by mass, carrying out colloid milling on the pulp for 2 times, diluting the pulp by 100 times, soaking the pulp for 20min at 55 ℃, filtering the pulp with 400-mesh filter cloth while the pulp is hot, and adding 1% of cellulase in the filtrate when the temperature of the filtrate is reduced to room temperature; the using amount of the decomposition liquid is 12 percent of the mass of the sarcandra glabra; the hawthorn is close to the mature period but not mature; the water content of the fructus choerospondiatis is 85%;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 30 hours, taking out the sarcandra glabra, heating the sarcandra glabra to the temperature of 5 ℃ by using microwaves, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 15 hours;
the cooling speed is 3 ℃/min;
(5) pulverizing
Putting the sarcandra glabra with ice into a grinder, grinding the sarcandra glabra into 100-mesh powder at the temperature of 3 ℃, then raising the temperature of the powder to 20 ℃, and treating the powder for 50min by using 800W ultrasonic waves;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 55 ℃ for 20min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 200r/min for 30 min; then filtering with 400-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with clear water 10 times the weight of the filter residue, heating to 90 deg.C, stirring for 30min, filtering with 300 mesh filter cloth while hot, cooling the filtrate to room temperature, mixing with the previous filtrate, concentrating under reduced pressure, and drying to obtain the extract.
The mass ratio of the mixture to the extraction solvent is 1: 5; the extraction solvent is formed by mixing ethanol and acetic acid according to the mass ratio of 1: 0.5.
Claims (9)
1. A preparation technology of sarcandra glabra extract with the function of clearing heat from throat is characterized by comprising the following steps:
(1) cleaning of
Picking up impurities from fresh herba Pileae Scriptae, soaking herba Pileae Scriptae in clear water, stirring at 20-30r/min for 15-20min, taking out, and draining;
(2) self-decomposition treatment of sarcandra glabra
Placing the sarcandra glabra processed in the previous step into a porcelain basin, compacting, sealing the surface of the porcelain basin by using a plastic film, covering the surface of the plastic film by using cotton cloth for 3-5cm, placing the porcelain basin into a large basin, injecting clear water into the large basin to enable the water surface to reach the central line of the porcelain basin, heating the water in the large basin to enable the water temperature to rise to 60-65 ℃, and preserving heat for 5-8 hours;
(3) herba Pileae Scriptae
Taking out herba Pileae Scriptae, recovering to room temperature, spraying decomposition liquid on the surface of herba Pileae Scriptae, heating to 40-45 deg.C, adjusting air humidity to 45-55%, and spreading for cooling for 30-50 min; stirring herba Pileae Scriptae, spraying decomposition solution again, and spreading for cooling for 20-30 min; then raising the temperature to 48-52 ℃, turning over sarcandra glabra, spraying decomposition liquid, and spreading for cooling for 4-7 h;
the preparation method of the decomposition liquid comprises the following steps: mixing and pulping 10-20 parts of hawthorn, 5-8 parts of fructus choerospondiatis and 8-12 parts of lemon in parts by mass, carrying out colloid milling on the pulp for 2-3 times, diluting by 50-100 times, soaking at 50-55 ℃ for 20-30min, filtering with 300-mesh 400-mesh filter cloth while the pulp is hot, and adding 1-3% of cellulase in the filtrate when the temperature of the filtrate is reduced to room temperature;
(4) freezing
Taking out the sarcandra glabra, placing the sarcandra glabra into a freezing chamber, reducing the temperature to minus 40 ℃, freezing for 20-30h, taking out the sarcandra glabra, heating to the temperature of 5-8 ℃ by using microwave, placing the sarcandra glabra into the freezing chamber again, reducing the temperature to minus 20 ℃, and freezing for 10-15 h;
(5) pulverizing
Putting the sarcandra glabra with ice into a crusher, crushing the sarcandra glabra into 80-100 meshes of powder at the temperature of 1-3 ℃, then raising the temperature of the powder to 20-25 ℃, and carrying out ultrasonic treatment for 30-50min by using 500-800W;
(6) extraction of
Mixing the mixture obtained in the last step with an extraction solvent, sealing and soaking at 50-55 ℃ for 20-30min, then reducing the temperature to room temperature, opening the mixed solution, and stirring at a stirring speed of 100-200r/min for 30-50 min; then filtering with 300-400-mesh filter cloth, and collecting the filtrate for later use; mixing the filter residue with clear water 10-15 times of the mass of the filter residue, heating to 80-90 ℃, stirring for 20-30min, filtering with 300-400 mesh filter cloth while hot, mixing with the previous filtrate when the temperature of the filtrate is reduced to room temperature, and concentrating and drying under reduced pressure to obtain the extract.
2. The technology for preparing sarcandra glabra extract for clearing heat from throats according to claim 1, wherein in the step (1), the mass ratio of sarcandra glabra to clear water is 1: 8-10.
3. The technology for preparing sarcandra glabra extract for clearing heat from throats of claim 1, wherein in the step (2), the water content of the cotton cloth is 65-75%, and the temperature is 35-38 ℃.
4. The technology for preparing sarcandra glabra extract for clearing heat from the throat according to claim 1, wherein in the step (3), the amount of the decomposition liquid is 8-12% of the mass of sarcandra glabra.
5. The technology for preparing sarcandra glabra extract for clearing heat from the throat according to claim 1, wherein in the step (3), the hawthorn is hawthorn which is close to the mature period but is not mature.
6. The preparation technology of sarcandra glabra extract with the function of clearing heat from throat as claimed in claim 1, wherein in the step (3), the water content of the fructus choerospondiatis is 75-85%.
7. The technology for preparing sarcandra glabra extract for clearing heat from the throat according to claim 1, wherein in the step (4), the cooling rate is 3 ℃/min.
8. The technology for preparing sarcandra glabra extract for clearing heat from throats of claim 1, wherein in the step (6), the mass ratio of the mixture to the extraction solvent is 1: 3-5.
9. The technology for preparing sarcandra glabra extract for clearing heat from throats of claim 1, wherein in the step (4), the extraction solvent is formed by mixing ethanol and acetic acid in a mass ratio of 1: 0.5-0.8.
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| Publication number | Priority date | Publication date | Assignee | Title |
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