CN115998636B - Gel mask containing recombinant humanized collagen and preparation method thereof - Google Patents
Gel mask containing recombinant humanized collagen and preparation method thereof Download PDFInfo
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Abstract
The invention is suitable for the technical field of mask production, and provides a gel mask containing recombinant humanized collagen and a preparation method thereof. The mask comprises the following raw materials: the invention has good hydrophilicity and activity by adding the recombinant humanized collagen, and can keep the moisture of skin cuticle; by adding the plant extract, various plant components are collected, so that the plant extract has good lubrication, moisturizing, anti-inflammatory and antibacterial effects; by adding the modified tourmaline, skin care ingredients and moisture can be fully adsorbed, a stable system is formed with other ingredients, and the modified tourmaline has good compatibility with skin and good water retention; the modified bamboo charcoal powder is added, so that the medicine and water storage space is large. The modified tourmaline and the modified bamboo charcoal powder have synergistic effect, and can further improve the moisturizing function of the mask.
Description
Technical Field
The invention belongs to the technical field of mask production, and particularly relates to a gel mask containing recombinant humanized collagen and a preparation method thereof.
Background
The facial mask is a kind of skin care product, is used for moisturizing skin, and also has multiple functions of moisturizing, nourishing, improving appearance, deep cleaning and the like. At present, most of common beauty masks on the market are facial masks such as essence and other effective skin care components are impregnated in non-woven fabrics, the skin care components on the surfaces of the non-woven fabrics are extremely easy to oxidize when exposed to air, moisture is extremely easy to evaporate, after the moisture evaporates, the non-woven fabrics can absorb moisture in skin, skin pores shrink, and nutrition components cannot be fully introduced into the skin.
Disclosure of Invention
The invention provides a gel mask containing recombinant humanized collagen and a preparation method thereof, and aims to solve the problems.
The invention is realized in such a way that the gel mask containing the recombinant humanized collagen comprises the following raw materials in parts by weight: 10-20 parts of recombinant humanized collagen, 20-30 parts of natural polymer, 6-10 parts of polyvinyl alcohol, 8-12 parts of plant extract, 5-9 parts of modified tourmaline, 4-8 parts of modified bamboo charcoal powder, 1-2 parts of antioxidant and 15-25 parts of deionized water.
The preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 800-900 ℃, roasting the mixture for 1-2 hours at constant temperature, cooling the mixture, and grinding the mixture to the particle size of 200-300 meshes; then adding 20-30vt% hydrochloric acid solution, and soaking for 3-5h; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight of 6-10 times, adding a 5-10wt% vinyl trimethoxysilane solution, and performing ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:5-9; then slowly adding the mixture into the mixture, heating to 60-80 ℃, stirring and reacting for 3-5 hours, filtering to obtain a solid product B, flushing the solid product B with purified water for 3-5 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 20-30 minutes under the conditions of 16-20kHz of power supply frequency, 20-30kV of working voltage and 80-90W of discharge power; taking out, adding mannitol 3-5 times of the weight, transferring into an ultrasonic instrument, performing ultrasonic treatment for 30-50min, filtering, and oven drying to obtain modified tourmaline.
Wherein, tourmaline can generate negative ions, plays a role in inhibiting the growth of bacteria, can regulate the ion balance of human body, relax body and mind and inhibit the aging of the body; meanwhile, tourmaline has far infrared radiation performance, can emit far infrared rays, can warm the face by releasing the far infrared rays, can promote blood circulation, can smooth metabolism, and can permeate skin care components into deep parts of the body. The tourmaline is roasted to remove water in different states in the crystal structure of the tourmaline, so that the internal structure becomes loose and porous, the specific surface area is increased, then the surface of the tourmaline is subjected to surface corrosion modification through hydrochloric acid, so that the surface of the tourmaline particles forms a stable porous structure, then the tourmaline with a large amount of hydroxyl groups adsorbed in the interior, the surface and the pore diameter is obtained through modification through the glycerol with polyhydroxy groups, finally the surface of the tourmaline particles is coarser after being treated through a low-temperature plasma treatment device, the specific surface area is further increased, a three-dimensional network space is formed, the skin care components and moisture can be fully adsorbed, a stable system is formed with other components, the compatibility with the skin is good, the water retention is good, the effective skin care components enter the skin, and the skin care effect is good.
Preferably, the material comprises the following raw materials in parts by weight: 12-18 parts of recombinant humanized collagen, 23-27 parts of natural polymer, 7-9 parts of polyvinyl alcohol, 9-11 parts of plant extract, 6-8 parts of modified tourmaline, 5-7 parts of modified bamboo charcoal powder, 1.2-1.8 parts of antioxidant and 18-22 parts of deionized water.
Preferably, the material comprises the following raw materials in parts by weight: 15 parts of recombinant humanized collagen, 25 parts of natural polymer, 8 parts of polyvinyl alcohol, 10 parts of plant extract, 7 parts of modified tourmaline, 6 parts of modified bamboo charcoal powder, 1.5 parts of antioxidant and 20 parts of deionized water.
Preferably, the natural polymer comprises one or more of alginate, chitosan, hyaluronic acid, galactomannan, phycocolloids, and xanthan gum.
Preferably, the plant extract is prepared by the following steps: weighing 1-3 parts by weight of olive leaves, 1-2 parts by weight of green tea, 3-5 parts by weight of evening primrose, 2-3 parts by weight of aloe and 1-3 parts by weight of chamomile, cleaning, airing, mashing, adding deionized water, stirring for 20-30min at 50-60 ℃, cooling to room temperature, putting into a percolator, performing percolation extraction by adopting an ethanol aqueous solution with 15-20 times of weight and 50-60% of volume concentration to obtain a percolation extract and a percolation extract residue, adding the percolation extract residue into an ethanol aqueous solution with 8-12 times of weight and 50-60% of volume concentration, soaking for 1-3h, performing ultrasonic extraction for 30-40min, filtering to obtain an ultrasonic extract and an ultrasonic extract residue, adding 6-10 times of water into the ultrasonic extract residue, putting into a high-pressure kettle, performing high-pressure decoction for 20-30min, filtering to obtain a high-pressure extract and a high-pressure extract residue, combining the percolation extract, the ultrasonic extract and the high-pressure extract, and concentrating the high-pressure extract under reduced pressure to 10-15% of the original volume, thereby obtaining the plant extract. The active composite component extracted from olive leaves is rich in antioxidants, and has good effects of smoothing skin fine lines and resisting aging; green tea has strong antibacterial and enzyme inhibiting effects; the evening primrose contains rich essential fatty acid and gamma-linoleic acid, and the seed essence thereof can inhibit the formation of melanin, prevent the precipitation of melanin, lighten color spots and even skin color; the aloe extract has good lubricating and moisturizing effects, and also has antiinflammatory, antiinflammatory and injured skin healing promoting effects; the flos Matricariae Chamomillae extract can improve sensitive, water-deficient and dry peeled skin.
Preferably, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 5-8 times of acetic acid for 3-6h, filtering, washing with deionized water, drying, grinding into nanometer powder, heating to 500-600deg.C, expanding with water vapor, cooling, washing with water, drying to obtain expanded bamboo charcoal powder, placing the expanded bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation broth, wrapping, standing at 25-30deg.C for 2-5 days, washing the culture product with deionized water, removing residual culture solution, soaking with deionized water to colorless, filtering, and drying to obtain modified bamboo charcoal powder; the surface of the bamboo charcoal powder is modified by acetic acid soaking, then the bamboo charcoal powder is reamed by water vapor, so that the bamboo charcoal powder is finer and more porous, the specific surface area is larger, bacterial cellulose grows into the bamboo charcoal fiber in the fermentation process, a staggered interpenetrating network is formed between the bacterial cellulose and the bamboo charcoal fiber, a large medicine and water storage space is provided, a good dissolution environment can be provided, the dual functions of rapid adsorption and diffusion of the bacterial cellulose are fully utilized, skin care components and water are adsorbed, and the bacterial cellulose can be rapidly diffused to the surface of skin in use.
Preferably, the preparation method of the bacterial cellulose fermentation broth comprises the following steps: inoculating the gluconacetobacter strains into the culture solution, and obtaining seed solution at the temperature of 25-30 ℃ and the stirring speed of 220-250r/min for 18-24 hours; inoculating 8-12% seed solution into the culture solution, and mixing to obtain bacterial cellulose fermentation liquor; wherein, the formula of the culture solution is as follows: glucose 2%, peptone 0.5%, yeast extract 0.5%, citric acid 0.1%, disodium hydrogen phosphate 0.2%, magnesium sulfate 0.1%, and water in balance.
Preferably, the antioxidant is tea polyphenol and/or apple tannin.
The invention also provides a preparation method of the gel mask containing the recombinant humanized collagen, which comprises the following steps:
s1: mixing recombinant humanized collagen, natural polymer, modified tourmaline, modified bamboo charcoal powder and half deionized water to obtain phase A;
s2: mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B;
s3: fully and uniformly stirring the phase A, the phase B and the antioxidant, and keeping the temperature of 70-90 ℃ for 30-60min to obtain a phase C;
s4: filling the C phase into a mask mold, cooling for 4-6h at-20 to-10 ℃, and thawing at room temperature to obtain the gel mask.
Compared with the prior art, the embodiment of the application has the following main beneficial effects:
the gel mask containing the recombinant humanized collagen provided by the invention has good hydrophilicity and activity by adding the recombinant humanized collagen, contains a large number of hydrophilic genes, has good film forming property, and keeps moisture of skin cuticle; by adding the plant extract, various plant components are collected, so that the plant extract has good lubrication, moisturizing, anti-inflammatory and antibacterial effects; the modified tourmaline is added to generate far infrared rays, the far infrared rays are released to enable the face to generate a warm effect, warm cells and promote blood circulation, skin care components can permeate into deep parts of the body, skin care components and moisture can be fully adsorbed, a stable system is formed with other components, the compatibility with the skin is good, the water retention is good, and the skin care effect is good; by adding the modified bamboo charcoal powder, the medicine and water storage space is large, and a good dissolution environment can be provided, so that skin care ingredients and water can be rapidly diffused to the skin surface. In addition, the modified tourmaline and the modified bamboo charcoal powder have a synergistic effect, and the moisturizing function of the facial mask can be further improved.
Drawings
FIG. 1 is a flow chart of the preparation of a gel mask containing recombinant humanized collagen;
Detailed Description
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
Example 1
The embodiment of the invention provides a gel mask containing recombinant humanized collagen, which is shown in figure 1, and the following raw materials in parts by weight are weighed: 10 parts of recombinant humanized collagen, 20 parts of natural polymers, 6 parts of polyvinyl alcohol, 8 parts of plant extracts, 5 parts of modified tourmaline, 4 parts of modified bamboo charcoal powder, 1 part of antioxidant and 15 parts of deionized water, and mixing the recombinant humanized collagen, the natural polymers, the modified tourmaline, the modified bamboo charcoal powder and half of deionized water to obtain a phase A; mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B; fully and uniformly stirring the phase A, the phase B and the antioxidant, and maintaining the temperature at 70 ℃ for 30min to obtain a phase C; filling the C phase into a mask mold, cooling at-20deg.C for 4 hr, and thawing at room temperature to obtain gel mask.
Wherein the natural polymer comprises alginate, chitosan, hyaluronic acid and the like which are mixed according to the mass ratio.
In this embodiment, the preparation method of the plant extract is as follows: 1 part by weight of olive leaves, 1 part by weight of green tea, 3 parts by weight of evening primrose, 2 parts by weight of aloe and 1 part by weight of chamomile are weighed, washed, aired and smashed, deionized water is added, stirring is carried out for 20min at 50 ℃, cooling is carried out to room temperature, the mixture is put into a percolator, an ethanol aqueous solution with the weight of 15 times and the volume concentration of 50% is adopted for carrying out percolation extraction, a percolation extraction solution and a percolation extraction residue are obtained, the percolation extraction residue is added with an ethanol aqueous solution with the weight of 8 times and the volume concentration of 50%, soaking is carried out for 1h, ultrasonic extraction is carried out for 30min, filtration is carried out, an ultrasonic extraction solution and an ultrasonic extraction residue are obtained, water with the weight of 6 times is added into an autoclave, high-pressure decoction is carried out for 20min, filtration is carried out, a high-pressure extraction solution and a high-pressure extraction residue are obtained, and the percolation extraction solution, the ultrasonic extraction solution and the high-pressure extraction solution are combined, and the reduced pressure evaporation concentration is carried out to 10% of the original volume, and the plant extract is obtained.
In specific implementation, the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 800 ℃, roasting the mixture at constant temperature for 1h, cooling the mixture, and grinding the mixture to the particle size of 200 meshes; then adding 20vt percent hydrochloric acid solution, and soaking for 3 hours; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight being 6 times that of the solid product A, adding a 5wt% vinyl trimethoxy silane solution, and performing ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:5; slowly adding the mixture into the mixture, heating to 60 ℃, stirring and reacting for 3 hours, filtering to obtain a solid product B, flushing the solid product B with purified water for 3 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 20 minutes under the conditions of 16kHz of power supply frequency, 20kV of working voltage and 80W of discharge power; taking out, adding mannitol 3 times of the weight of the modified tourmaline, transferring into an ultrasonic instrument, performing ultrasonic treatment for 30min, filtering and drying.
Further, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 5 times of acetic acid for 3 hours, taking out, filtering, washing with deionized water, drying, grinding into nano powder, heating to 500 ℃, then introducing water vapor for reaming, cooling, washing with water and drying to obtain reaming bamboo charcoal powder, loading the reaming bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation liquor, wrapping, standing at 25 ℃ for 2 days, rinsing the culture product with deionized water, removing residual culture solution, soaking with deionized water until the culture solution is colorless, and finally filtering and drying to obtain the modified bamboo charcoal powder.
The preparation method of the bacterial cellulose fermentation liquor comprises the following steps: inoculating gluconacetobacter strains into the culture solution, and obtaining seed solution at the temperature of 25 ℃ and the stirring speed of 220r/min for 18 hours; and (3) inoculating 8% of seed liquid into the culture solution, and uniformly mixing to obtain bacterial cellulose fermentation liquor.
In this embodiment, the antioxidant is tea polyphenol.
Example 2
The embodiment of the invention provides a gel mask containing recombinant humanized collagen, which is shown in figure 1, and the following raw materials in parts by weight are weighed: 12 parts of recombinant humanized collagen, 23 parts of natural polymers, 7 parts of polyvinyl alcohol, 9 parts of plant extracts, 6 parts of modified tourmaline, 5 parts of modified bamboo charcoal powder, 1.2 parts of antioxidants and 18 parts of deionized water, and mixing the recombinant humanized collagen, the natural polymers, the modified tourmaline, the modified bamboo charcoal powder and half of deionized water to obtain a phase A; mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B; fully and uniformly stirring the phase A, the phase B and the antioxidant, and maintaining the temperature at 70 ℃ for 30min to obtain a phase C; filling the C phase into a mask mold, cooling at-20deg.C for 4 hr, and thawing at room temperature to obtain gel mask.
Wherein the natural polymer comprises galactomannan, phycocolloids, xanthan gum and the like which are mixed according to the mass ratio.
In this embodiment, the preparation method of the plant extract is as follows: 1 part by weight of olive leaves, 1 part by weight of green tea, 3 parts by weight of evening primrose, 2 parts by weight of aloe and 1 part by weight of chamomile are weighed, washed, aired and smashed, deionized water is added, stirring is carried out for 20min at 50 ℃, cooling is carried out to room temperature, the mixture is put into a percolator, an ethanol aqueous solution with the weight of 15 times and the volume concentration of 50% is adopted for carrying out percolation extraction, a percolation extraction solution and a percolation extraction residue are obtained, the percolation extraction residue is added with an ethanol aqueous solution with the weight of 8 times and the volume concentration of 50%, soaking is carried out for 1h, ultrasonic extraction is carried out for 30min, filtration is carried out, an ultrasonic extraction solution and an ultrasonic extraction residue are obtained, water with the weight of 6 times is added into an autoclave, high-pressure decoction is carried out for 20min, filtration is carried out, a high-pressure extraction solution and a high-pressure extraction residue are obtained, and the percolation extraction solution, the ultrasonic extraction solution and the high-pressure extraction solution are combined, and the reduced pressure evaporation concentration is carried out to 10% of the original volume, and the plant extract is obtained.
In specific implementation, the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 800 ℃, roasting the mixture at constant temperature for 1h, cooling the mixture, and grinding the mixture to the particle size of 200 meshes; then adding 20vt percent hydrochloric acid solution, and soaking for 3 hours; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight being 6 times that of the solid product A, adding a 5wt% vinyl trimethoxy silane solution, and performing ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:5; slowly adding the mixture into the mixture, heating to 60 ℃, stirring and reacting for 3 hours, filtering to obtain a solid product B, flushing the solid product B with purified water for 3 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 20 minutes under the conditions of 16kHz of power supply frequency, 20kV of working voltage and 80W of discharge power; taking out, adding mannitol 3 times of the weight of the modified tourmaline, transferring into an ultrasonic instrument, performing ultrasonic treatment for 30min, filtering and drying.
Further, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 5 times of acetic acid for 3 hours, taking out, filtering, washing with deionized water, drying, grinding into nano powder, heating to 500 ℃, then introducing water vapor for reaming, cooling, washing with water and drying to obtain reaming bamboo charcoal powder, loading the reaming bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation liquor, wrapping, standing at 25 ℃ for 2 days, rinsing the culture product with deionized water, removing residual culture solution, soaking with deionized water until the culture solution is colorless, and finally filtering and drying to obtain the modified bamboo charcoal powder.
The preparation method of the bacterial cellulose fermentation liquor comprises the following steps: inoculating gluconacetobacter strains into the culture solution, and obtaining seed solution at the temperature of 25 ℃ and the stirring speed of 220r/min for 18 hours; and (3) inoculating 8% of seed liquid into the culture solution, and uniformly mixing to obtain bacterial cellulose fermentation liquor.
In this embodiment, the antioxidant is apple Shan Ningsu.
Example 3
The embodiment of the invention provides a gel mask containing recombinant humanized collagen, which is shown in figure 1, and the following raw materials in parts by weight are weighed: 15 parts of recombinant humanized collagen, 25 parts of natural polymers, 8 parts of polyvinyl alcohol, 10 parts of plant extracts, 7 parts of modified tourmaline, 6 parts of modified bamboo charcoal powder, 1.5 parts of antioxidants and 20 parts of deionized water, and mixing the recombinant humanized collagen, the natural polymers, the modified tourmaline, the modified bamboo charcoal powder and half of deionized water to obtain a phase A; mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B; fully and uniformly stirring the phase A, the phase B and the antioxidant, and keeping the temperature of 80 ℃ for 45min to obtain a phase C; filling the C phase into a mask mold, cooling at-15deg.C for 5 hr, and thawing at room temperature to obtain gel mask.
Wherein the natural polymer comprises chitosan, hyaluronic acid, galactomannan and the like which are mixed according to the mass ratio.
In this embodiment, the preparation method of the plant extract is as follows: 1-3 parts by weight of olive leaves, 1.5 parts by weight of green tea, 4 parts by weight of evening primrose, 2.5 parts by weight of aloe and 2 parts by weight of chamomile are weighed, washed, aired and smashed, deionized water is added, stirring is carried out for 25min at 55 ℃, cooling is carried out to room temperature, the mixture is put into a percolator, an ethanol aqueous solution with the weight of 18 times and the volume concentration of 55% is adopted for carrying out percolation extraction, a percolation extraction solution and a percolation extraction residue are obtained, the percolation extraction residue is added with an ethanol aqueous solution with the weight of 10 times and the volume concentration of 55% and is soaked for 2h, ultrasonic extraction is carried out for 35min, filtration is carried out, an ultrasonic extraction solution and an ultrasonic extraction residue are obtained, the ultrasonic extraction residue is added with 8 times of water, high-pressure decoction is carried out for 25min, filtration is carried out, a high-pressure extraction solution and a high-pressure extraction residue are obtained, the percolation extraction solution, the ultrasonic extraction solution and the high-pressure extraction solution are combined, and the high-pressure extraction solution is evaporated and concentrated to be 12% of the original volume, and the plant extract is obtained.
In specific implementation, the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 850 ℃, roasting the mixture at constant temperature for 1.5 hours, cooling the mixture, and grinding the mixture to the particle size of 250 meshes; then adding 25vt percent hydrochloric acid solution, and soaking for 4 hours; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight being 8 times that of the solid product A, adding 8wt% of vinyl trimethoxy silane solution, and carrying out ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:7; slowly adding the mixture into the mixture, heating to 70 ℃, stirring and reacting for 4 hours, filtering to obtain a solid product B, washing the solid product B with purified water for 4 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 25 minutes under the conditions of 18kHz of power supply frequency, 25kV of working voltage and 85W of discharge power; taking out, adding mannitol 4 times of the weight, transferring into an ultrasonic instrument, performing ultrasonic treatment for 40min, filtering, and oven drying to obtain modified tourmaline.
Further, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 5-8 times of acetic acid for 4.5 hr, filtering, washing with deionized water, drying, grinding into nanometer powder, heating to 550deg.C, reaming with steam, cooling, washing with water, drying to obtain reaming bamboo charcoal powder, loading reaming bamboo charcoal powder into culture vessel, adding bacterial cellulose fermentation broth, wrapping, standing at 28deg.C for 3.5 days, washing the culture product with deionized water, removing residual culture solution, soaking with deionized water to colorless, filtering, and drying to obtain modified bamboo charcoal powder.
The preparation method of the bacterial cellulose fermentation liquor comprises the following steps: inoculating gluconacetobacter strains into the culture solution, and obtaining seed solution after culturing for 21 hours at the temperature of 27 ℃ and the stirring speed of 235 r/min; and (3) inoculating seed liquid with the volume of 10% of that of the culture liquid into the culture liquid, and uniformly mixing for later use to obtain bacterial cellulose fermentation liquor.
In this embodiment, the antioxidant is a mixture of tea polyphenols and apples Shan Ningsu in a mass ratio.
Example 4
The embodiment of the invention provides a gel mask containing recombinant humanized collagen, which is shown in figure 1, and the following raw materials in parts by weight are weighed: 18 parts of recombinant humanized collagen, 27 parts of natural polymer, 9 parts of polyvinyl alcohol, 11 parts of plant extract, 8 parts of modified tourmaline, 7 parts of modified bamboo charcoal powder, 1.8 parts of antioxidant and 22 parts of deionized water, and mixing the recombinant humanized collagen, the natural polymer, the modified tourmaline, the modified bamboo charcoal powder and half of deionized water to obtain phase A; mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B; fully and uniformly stirring the phase A, the phase B and the antioxidant, and keeping the temperature of 90 ℃ for 60min to obtain a phase C; filling the C phase into a mask mold, cooling at-10deg.C for 6h, and thawing at room temperature to obtain gel mask.
Wherein the natural polymer comprises xanthan gum.
In this embodiment, the preparation method of the plant extract is as follows: weighing 3 parts by weight of olive leaves, 2 parts by weight of green tea, 5 parts by weight of evening primrose, 3 parts by weight of aloe and 3 parts by weight of chamomile, cleaning, airing, mashing, adding deionized water, stirring at 60 ℃ for 30min, cooling to room temperature, putting into a percolator, performing percolating extraction by adopting an ethanol water solution with the weight of 20 times and the volume concentration of 60% to obtain a percolating extraction liquid and a percolating extraction residue, adding an ethanol water solution with the weight of 12 times and the volume concentration of 60% into the percolating extraction residue, soaking for 3h, performing ultrasonic extraction for 40min, filtering to obtain an ultrasonic extraction liquid and an ultrasonic extraction residue, adding 10 times of water into the ultrasonic extraction residue, putting into an autoclave, performing high-pressure decoction for 30min, filtering to obtain a high-pressure extraction liquid and a high-pressure extraction residue, merging the percolating extraction liquid, the ultrasonic extraction liquid and the high-pressure extraction liquid, and performing reduced evaporation concentration to 15% of the original volume, and obtaining the plant extract.
In specific implementation, the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 900 ℃, roasting the mixture at constant temperature for 2 hours, cooling the mixture, and grinding the mixture to the particle size of 300 meshes; then adding 30vt percent hydrochloric acid solution, and soaking for 5 hours; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight being 10 times that of the solid product A, adding a 10wt% vinyl trimethoxy silane solution, and carrying out ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:9; slowly adding the mixture into the mixture, heating to 80 ℃, stirring and reacting for 5 hours, filtering to obtain a solid product B, washing the solid product B with purified water for 5 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 30 minutes under the conditions of 20kHz of power supply frequency, 30kV of working voltage and 90W of discharge power; taking out, adding mannitol 5 times of the weight of the modified tourmaline, transferring into an ultrasonic instrument, performing ultrasonic treatment for 50min, filtering and drying.
Further, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 8 times of acetic acid for 6 hours, taking out, filtering, washing with deionized water, drying, grinding into nano powder, heating to 600 ℃, then, introducing steam for reaming, cooling, washing with water and drying to obtain reaming bamboo charcoal powder, loading the reaming bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation liquor, wrapping, standing at 30 ℃ for 5 days, rinsing the culture product with deionized water, removing residual culture solution, soaking with deionized water until the culture solution is colorless, and finally filtering and drying to obtain the modified bamboo charcoal powder.
The preparation method of the bacterial cellulose fermentation liquor comprises the following steps: inoculating gluconacetobacter strains into the culture solution, and obtaining seed solution after the culture for 24 hours at the temperature of 30 ℃ and the stirring speed of 250 r/min; and (3) inoculating the seed liquid with the volume of 12% of that of the culture liquid into the culture liquid, and uniformly mixing for later use to obtain bacterial cellulose fermentation liquor.
In this embodiment, the antioxidant is tea polyphenol.
Example 5
The embodiment of the invention provides a gel mask containing recombinant humanized collagen, which is shown in figure 1, and the following raw materials in parts by weight are weighed: 20 parts of recombinant humanized collagen, 30 parts of natural polymers, 10 parts of polyvinyl alcohol, 12 parts of plant extracts, 9 parts of modified tourmaline, 8 parts of modified bamboo charcoal powder, 2 parts of antioxidants and 25 parts of deionized water, and mixing the recombinant humanized collagen, the natural polymers, the modified tourmaline, the modified bamboo charcoal powder and half of deionized water to obtain a phase A; mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B; fully and uniformly stirring the phase A, the phase B and the antioxidant, and keeping the temperature of 90 ℃ for 60min to obtain a phase C; filling the C phase into a mask mold, cooling at-10deg.C for 6h, and thawing at room temperature to obtain gel mask.
Wherein the natural polymer comprises hyaluronic acid.
In this embodiment, the preparation method of the plant extract is as follows: weighing 3 parts by weight of olive leaves, 2 parts by weight of green tea, 5 parts by weight of evening primrose, 3 parts by weight of aloe and 3 parts by weight of chamomile, cleaning, airing, mashing, adding deionized water, stirring at 60 ℃ for 30min, cooling to room temperature, putting into a percolator, performing percolating extraction by adopting an ethanol water solution with the weight of 20 times and the volume concentration of 60% to obtain a percolating extraction liquid and a percolating extraction residue, adding an ethanol water solution with the weight of 12 times and the volume concentration of 60% into the percolating extraction residue, soaking for 3h, performing ultrasonic extraction for 40min, filtering to obtain an ultrasonic extraction liquid and an ultrasonic extraction residue, adding 10 times of water into the ultrasonic extraction residue, putting into an autoclave, performing high-pressure decoction for 30min, filtering to obtain a high-pressure extraction liquid and a high-pressure extraction residue, merging the percolating extraction liquid, the ultrasonic extraction liquid and the high-pressure extraction liquid, and performing reduced evaporation concentration to 15% of the original volume, and obtaining the plant extract.
In specific implementation, the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 900 ℃, roasting the mixture at constant temperature for 2 hours, cooling the mixture, and grinding the mixture to the particle size of 300 meshes; then adding 30vt percent hydrochloric acid solution, and soaking for 5 hours; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight being 10 times that of the solid product A, adding a 10wt% vinyl trimethoxy silane solution, and carrying out ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:9; slowly adding the mixture into the mixture, heating to 80 ℃, stirring and reacting for 5 hours, filtering to obtain a solid product B, washing the solid product B with purified water for 5 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 30 minutes under the conditions of 20kHz of power supply frequency, 30kV of working voltage and 90W of discharge power; taking out, adding mannitol 5 times of the weight of the modified tourmaline, transferring into an ultrasonic instrument, performing ultrasonic treatment for 50min, filtering and drying.
Further, the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 8 times of acetic acid for 6 hours, taking out, filtering, washing with deionized water, drying, grinding into nano powder, heating to 600 ℃, then, introducing steam for reaming, cooling, washing with water and drying to obtain reaming bamboo charcoal powder, loading the reaming bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation liquor, wrapping, standing at 30 ℃ for 5 days, rinsing the culture product with deionized water, removing residual culture solution, soaking with deionized water until the culture solution is colorless, and finally filtering and drying to obtain the modified bamboo charcoal powder.
The preparation method of the bacterial cellulose fermentation liquor comprises the following steps: inoculating gluconacetobacter strains into the culture solution, and obtaining seed solution after the culture for 24 hours at the temperature of 30 ℃ and the stirring speed of 250 r/min; and (3) inoculating the seed liquid with the volume of 12% of that of the culture liquid into the culture liquid, and uniformly mixing for later use to obtain bacterial cellulose fermentation liquor.
In this embodiment, the antioxidant is apple Shan Ningsu.
Comparative example 1: the difference from example 3 is that the modified tourmaline is replaced with the general tourmaline.
Comparative example 2: the difference from example 3 is that the modified bamboo charcoal powder was replaced with a normal bamboo charcoal powder.
Comparative example 3: the difference from example 3 is that modified tourmaline was replaced with normal tourmaline and modified bamboo charcoal powder was replaced with normal bamboo charcoal powder.
Comparative example 4: a commercially available gel facial mask.
Skin moisture content determination: 90 testers were randomly selected and divided into 9 groups (examples 1-5 and comparative examples 1-4) of 10 persons each, and skin moisture content was measured using a skin diagnostician MC760 skin tester from CK company, germany, and the mask samples of the above examples and comparative examples were applied to the skin (about 8 square cm) on the inner side of the forearm of the subject, and were removed after 30 minutes.
Skin moisture content was measured before use and after 30, 60, 90 and 120min after use. The change of the moisture content is reflected in the test period, and the change rule of the moisture content of the experimental area along with time. The larger the value, the larger the moisture content, and conversely, the smaller the moisture content. Hydration = (moisture content at each time point after use-initial moisture content)/initial moisture content, test results are shown in the following table:
in summary, the invention provides a gel mask containing recombinant humanized collagen, and the results show that the gel mask prepared by the invention has good moisturizing effect, and the modified tourmaline and the modified bamboo charcoal powder have synergistic effect, so that the moisturizing function of the mask can be further improved, and the gel mask has very broad market prospect.
The above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention. It will be apparent that the described embodiments are merely some, but not all, embodiments of the invention. Based on these embodiments, all other embodiments that may be obtained by one of ordinary skill in the art without inventive effort are within the scope of the invention.
Claims (6)
1. The gel mask containing the recombinant humanized collagen is characterized by comprising the following raw materials in parts by weight: 10-20 parts of recombinant humanized collagen, 20-30 parts of natural polymer, 6-10 parts of polyvinyl alcohol, 8-12 parts of plant extract, 5-9 parts of modified tourmaline, 4-8 parts of modified bamboo charcoal powder, 1-2 parts of antioxidant and 15-25 parts of deionized water;
the preparation method of the plant extract comprises the following steps: weighing 1-3 parts by weight of olive leaves, 1-2 parts by weight of green tea, 3-5 parts by weight of evening primrose, 2-3 parts by weight of aloe and 1-3 parts by weight of chamomile, cleaning, airing, mashing, adding deionized water, stirring for 20-30min at 50-60 ℃, cooling to room temperature, putting into a percolator, performing percolation extraction by adopting an ethanol aqueous solution with 15-20 times of weight and 50-60% of volume concentration to obtain a percolation extract and a percolation extract residue, adding the percolation extract residue into an ethanol aqueous solution with 8-12 times of weight and 50-60% of volume concentration, soaking for 1-3h, performing ultrasonic extraction for 30-40min, filtering to obtain an ultrasonic extract and an ultrasonic extract residue, adding water with 6-10 times of weight into the ultrasonic extract residue, putting into an autoclave, performing high-pressure decoction for 20-30min, filtering to obtain a high-pressure extract and a high-pressure extract residue, combining the percolation extract, the ultrasonic extract and the high-pressure extract, and concentrating the percolation extract into 10-15% of the original volume by evaporation to obtain the plant extract;
the preparation method of the modified tourmaline comprises the following steps: cleaning the surface of tourmaline, drying and then primarily crushing; then placing the mixture in a muffle furnace, programming the temperature to 800-900 ℃, roasting the mixture for 1-2 hours at constant temperature, cooling the mixture, and grinding the mixture to the particle size of 200-300 meshes; then adding 20-30vt% hydrochloric acid solution, and soaking for 3-5h; filtering to obtain a solid product A, adding the solid product A into deionized water with the weight of 6-10 times, adding a 5-10wt% vinyl trimethoxysilane solution, and performing ultrasonic dispersion until the mixture is uniform to obtain a mixture; mixing glycerol and deionized water according to a mass ratio of 1:5-9; then slowly adding the mixture into the mixture, heating to 60-80 ℃, stirring and reacting for 3-5 hours, filtering to obtain a solid product B, flushing the solid product B with purified water for 3-5 times, placing the solid product B in a drying oven, performing decompression drying treatment, uniformly spreading the solid product B on an electrode in a low-temperature plasma treatment device, and treating the solid product B for 20-30 minutes under the conditions of 16-20kHz of power supply frequency, 20-30kV of working voltage and 80-90W of discharge power; taking out, adding mannitol 3-5 times of the weight of the modified tourmaline, transferring into an ultrasonic instrument, performing ultrasonic treatment for 30-50min, filtering, and drying to obtain modified tourmaline;
the preparation method of the modified bamboo charcoal powder comprises the following steps: soaking bamboo charcoal powder in 5-8 times of acetic acid for 3-6h, filtering, washing with deionized water, drying, grinding into nanometer powder, heating to 500-600deg.C, expanding with water vapor, cooling, washing with water, drying to obtain expanded bamboo charcoal powder, placing the expanded bamboo charcoal powder into a culture vessel, injecting bacterial cellulose fermentation broth, wrapping, standing at 25-30deg.C for 2-5 days, washing the culture product with deionized water, removing residual culture solution, soaking with deionized water to colorless, filtering, and drying to obtain modified bamboo charcoal powder;
the preparation method of the bacterial cellulose fermentation broth comprises the following steps: inoculating the gluconacetobacter strains into the culture solution, and obtaining seed solution at the temperature of 25-30 ℃ and the stirring speed of 220-250r/min for 18-24 hours; inoculating 8-12% seed solution into the culture solution, and mixing to obtain bacterial cellulose fermentation liquor.
2. The gel mask containing recombinant humanized collagen according to claim 1, which comprises the following raw materials in parts by weight: 12-18 parts of recombinant humanized collagen, 23-27 parts of natural polymer, 7-9 parts of polyvinyl alcohol, 9-11 parts of plant extract, 6-8 parts of modified tourmaline, 5-7 parts of modified bamboo charcoal powder, 1.2-1.8 parts of antioxidant and 18-22 parts of deionized water.
3. The gel mask containing recombinant humanized collagen according to claim 2, which comprises the following raw materials in parts by weight: 15 parts of recombinant humanized collagen, 25 parts of natural polymer, 8 parts of polyvinyl alcohol, 10 parts of plant extract, 7 parts of modified tourmaline, 6 parts of modified bamboo charcoal powder, 1.5 parts of antioxidant and 20 parts of deionized water.
4. The gel mask containing recombinant humanized collagen according to claim 1, wherein the natural polymer comprises one or more of alginate, chitosan, hyaluronic acid, galactomannan, phycocolloid, xanthan gum.
5. The gel mask comprising recombinant humanized collagen according to claim 1, wherein the antioxidant is tea polyphenol and/or apple tannin.
6. The method for preparing a gel mask containing recombinant humanized collagen according to any one of claims 1 to 5, comprising the steps of:
s1: mixing recombinant humanized collagen, natural polymer, modified tourmaline, modified bamboo charcoal powder and half deionized water to obtain phase A;
s2: mixing polyvinyl alcohol, plant extract and the rest deionized water to obtain phase B;
s3: fully and uniformly stirring the phase A, the phase B and the antioxidant, and keeping the temperature of 70-90 ℃ for 30-60min to obtain a phase C;
s4: filling the C phase into a mask mold, cooling for 4-6h at-20 to-10 ℃, and thawing at room temperature to obtain the gel mask.
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