CN103340819A - Preparation method of bacterial cellulose facial mask with anti-radiation effect - Google Patents
Preparation method of bacterial cellulose facial mask with anti-radiation effect Download PDFInfo
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Abstract
The invention discloses a preparation method of a bacterial cellulose facial mask with an anti-radiation effect, and relates to a mask material preparation technique. The preparation method comprises the following steps of: selecting strains capable of secreting bacterial cellulose to be activated and prepared into a seed mash, subsequently and uniformly introducing the seed mash with strain concentration of 30-90wt% into a fermentation culture medium containing a plant general flavone ethanol solution, fermenting and culturing for 3-7 days and taking out a fermentation product, carrying out purification treatment on the fermentation product, and cutting so as to obtain the bacterial cellulose facial mask with a uniformly distributed internal bacterial cellulose three-dimensional network. The preparation method is environment-friendly in preparation process, simple and feasible, convenient to operate and low in cost, and the prepared facial mask is good in water binding capacity and air permeability and has a good anti-radiation effect.
Description
Technical field
The present invention relates to a kind of face-mask material technology of preparing, particularly a kind of preparation method with Bacterial cellulose facial film of radioprotective effect.
Background technology
The skin long term exposure all can make the human body skin surface produce reactive oxygen free radical in the strong daylight of living environment, ultraviolet, electromagnetic radiation according to the study.Excessive reactive oxygen free radical can generate brown pigment with effects such as unsaturated fatty acid, protein in the body, and being deposited in just becomes various mottles on the skin.In addition, the formed lipid peroxide of excessive free radicals also can be damaged biomembrane, destroy cell, hinder normal metabolic, accelerate aging, and can cause such as multiple diseases such as cancer, leukemia, hypertension, myocardial infarction, diabetes, hepatitis, gout, nephritis, cataract.Along with the mankind are more and more higher for the frequency of utilization of household appliances such as TV, computer, smart mobile phone, and electromagnetic radiation mainly concentrates on face, and the especially facial injury of skin of therefore adopting the too much reactive oxygen free radical reduction radiation of effective means elimination to cause necessitates.
Along with improving constantly and scientific and technical continuous development of people's living standard, the category of cosmetics is in continuous increase in recent years, and scientific and technological content also progressively improves.Wherein the mask product utilization covers the blink of skin surface, temporary transient isolation outside air and pollution, improve the skin temperature, the pore expansion of skin, promote sweat gland secretion and metabolism, the skin oxygen content is risen, can effectively get rid of the oils and fats thing of skin toxin and accumulation, and fast with the horny layer of infiltration skin epidermises such as moisture, nutrient substance, make that skin becomes more soft, light is flexible.Utilize that facial film is easy to use, effective, the characteristics of quick skin protection are combined the mask product that preparation has a radioprotective effect and have huge market value with material that can the elimination activity oxygen-derived free radicals.
Be rich in chemical constituent flavone, alcohol, phenol, steroidal compounds etc. in the plant, these materials have very strong antioxidation, reach the effect that prevents irradiation thereby can eliminate too much reactive oxygen free radical.Bacterial cellulose (BC) is a kind of natural biopolymer, higher biocompatibility, adaptability and good biodegradability are arranged, and have many good physics, chemistry and mechanical performances such as high-crystallinity, high-purity, high retentiveness, superfine nano fibrillar meshwork structure.It has good ductility and good with the human body skin adhesion, is the primary raw material of present biology cellulose facial film.
This patent adopts ultrasonic method to extract total flavones from natural plants, and utilize the alcoholic solution that contains total flavones as culture medium, the biofermentation bacteria cellulose material is prepared and can be eliminated the Bacterial cellulose facial film that facial too much reactive oxygen free radical has the radioprotective effect.Preparation process environmental protection of the present invention, simple, easy to operate, cost is low, the facial film retentiveness and the good permeability that obtain have good radioprotective effect.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method with Bacterial cellulose facial film of radioprotective effect.Relate to a kind of face-mask material technology of preparing.Preparation process environmental protection of the present invention, simple, easy to operate, cost is low, the facial film retentiveness and the good permeability that obtain have good moisture preserving, radioprotective effect.
Of the present invention a kind of preparation method with Bacterial cellulose facial film of radioprotective effect disclosed, comprise: choose and the cellulosic bacterial strain activation of secreting bacteria to be prepared into the seed mash, the even access of seed mash that with bacterial strain concentration is 30～90wt% then contained in the fermentation medium of plant class general flavone ethanol solution, take out tunning behind fermentation culture 3～7d, the purified processing of tunning, cutting, the Bacterial cellulose facial film that obtains having the radioprotective effect.
The cellulosic bacterial strain of described energy secreting bacteria is one or more in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter.
The described plant class general flavone ethanol solution that contains is to be joined in the alcoholic solution and by supersound process by in Radix et Caulis Opuntiae Dillenii, Aloe, Folium Camelliae sinensis, Semen Ginkgo, the Folium Bambusae plant lyophilized powder one or more to obtain.
Described supersound process treatment step is:
(1) one or more addings in Radix et Caulis Opuntiae Dillenii, Aloe, Folium Camelliae sinensis, Semen Ginkgo, the Folium Bambusae plant lyophilized powder are accounted in the ethanol water of 20～50 times of plant lyophilized powder weight, wherein ethanol water concentration is 40～80wt%;
(2) with above-mentioned solution ultrasonic 5～40min under the ultrasonic power of 100～800W, remove by filter filtering residue;
(3) add distilled water in filtrate filtered, the ethanol content of regulating in the filtrate is 5～15wt%, obtains containing plant class general flavone ethanol solution.
Described fermentation medium is: contain glucose 10～30g, sodium hydrogen phosphate 1～4g, potassium dihydrogen phosphate 1～4g, yeast extract 1～5g, peptone 1～5g in the plant class general flavone ethanol solution of every 1L.
Described purification treating method is, tunning heats 3～6h under 30～100 ℃ temperature in the quality percentage composition is 1～8% NaOH aqueous solution, and water washes repeatedly to neutrality again.
Compared with prior art, the invention has the beneficial effects as follows: utilize total flavones can be dissolved in the characteristics of alcohols material in this patent, adopt the supersound process method from natural plants, to extract plant class total flavones with ethanol as extractant, and utilize and contain the alcoholic solution of total flavones as culture medium, in incubation, ethanol participates in the biosynthesis of Bacterial cellulose as the carbon source in the culture medium simultaneously.In the biological fermentation process of bacterial strain, ethanol is generated for the synthetic chemical compounds such as ATP of bacterial strain simultaneously by the bacterial strain oxidation, total flavones in the ethanol and the molecule of Bacterial cellulose produce bonding action, thereby at the inner evenly distribution total flavones molecule of Bacterial cellulose three-dimensional network.The biofermentation bacteria cellulose material is prepared and can be eliminated the Bacterial cellulose facial film that facial too much reactive oxygen free radical has the radioprotective effect.Preparation process environmental protection of the present invention, simple, easy to operate, cost is low, the facial film retentiveness and the good permeability that obtain have good radioprotective effect.This patent selects for use pure natural plants as the source of extracting total flavones, and this kind of plant class total flavones has very strong antioxidation, reaches the effect that prevents irradiation thereby can eliminate too much reactive oxygen free radical.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1:
1, the adding of Radix et Caulis Opuntiae Dillenii lyophilized powder is accounted in the ethanol water of 20 times of lyophilized powder weight, wherein ethanol water concentration is 80wt%; With above-mentioned solution ultrasonic 40min under the ultrasonic power of 100W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 15wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 10g, sodium hydrogen phosphate 1g, potassium dihydrogen phosphate 1g, yeast extract 5g, peptone 1g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choosing and can the cellulosic acetobacter xylinum activation of secreting bacteria be prepared into the seed mash, is that even access of seed mash of 30wt% contained in the fermentation medium of plant class general flavone ethanol solution then with bacterial strain concentration, takes out tunning behind the fermentation culture 7d.
4, tunning heats 6h under 80 ℃ temperature in the quality percentage composition is 3% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Embodiment 2:
1, the Aloe lyphillized powder adding is accounted in the ethanol water of 30 times of lyophilized powder weight, wherein ethanol water concentration is 70wt%; With above-mentioned solution ultrasonic 35min under the ultrasonic power of 200W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 10wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 20g, sodium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, yeast extract 3g, peptone 3g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choosing and can the cellulosic Sarcina activation of secreting bacteria be prepared into the seed mash, is that even access of seed mash of 50wt% contained in the fermentation medium of plant class general flavone ethanol solution then with bacterial strain concentration, takes out tunning behind the fermentation culture 5d.
4, tunning heats 6h under 100 ℃ temperature in the quality percentage composition is 2% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Embodiment 3:
1, the adding of freeze drying powder is accounted in the ethanol water of 40 times of lyophilized powder weight, wherein ethanol water concentration is 60wt%; With above-mentioned solution ultrasonic 30min under the ultrasonic power of 300W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 5wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 30g, sodium hydrogen phosphate 4g, potassium dihydrogen phosphate 4g, yeast extract 1g, peptone 5g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choosing and can the cellulosic achromobacter activation of secreting bacteria be prepared into the seed mash, is that even access of seed mash of 70wt% contained in the fermentation medium of plant class general flavone ethanol solution then with bacterial strain concentration, takes out tunning behind the fermentation culture 4d.
4, tunning heats 3h under 90 ℃ temperature in the quality percentage composition is 1% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Embodiment 4:
1, Semen Ginkgo and the adding of Folium Bambusae lyophilized powder are accounted in the ethanol water of 50 times of lyophilized powder weight, wherein ethanol water concentration is 50wt%; With above-mentioned solution ultrasonic 20min under the ultrasonic power of 500W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 15wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 10g, sodium hydrogen phosphate 3g, potassium dihydrogen phosphate 3g, yeast extract 2g, peptone 2g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choose the activation of the energy cellulosic Aerobacter of secreting bacteria and azotobacter and be prepared into the seed mash, the even access of seed mash that with bacterial strain concentration is 90wt% then contained in the fermentation medium of plant class general flavone ethanol solution, takes out tunning behind the fermentation culture 3d.
4, tunning heats 4h under 50 ℃ temperature in the quality percentage composition is 5% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Embodiment 5:
1, Radix et Caulis Opuntiae Dillenii and Aloe lyphillized powder adding are accounted in the ethanol water of 30 times of lyophilized powder weight, wherein ethanol water concentration is 40wt%; With above-mentioned solution ultrasonic 10min under the ultrasonic power of 600W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 10wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 20g, sodium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, yeast extract 4g, peptone 4g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choose the activation of the energy cellulosic acetobacter xylinum of secreting bacteria and Alcaligenes and be prepared into the seed mash, the even access of seed mash that with bacterial strain concentration is 80wt% then contained in the fermentation medium of plant class general flavone ethanol solution, takes out tunning behind the fermentation culture 7d.
4, tunning heats 6h under 30 ℃ temperature in the quality percentage composition is 8% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Embodiment 6:
1, Radix et Caulis Opuntiae Dillenii, Folium Camelliae sinensis and the adding of Folium Bambusae lyophilized powder are accounted in the ethanol water of 40 times of lyophilized powder weight, wherein ethanol water concentration is 50wt%; With above-mentioned solution ultrasonic 5min under the ultrasonic power of 800W, remove by filter filtering residue; Add distilled water in filtrate, the ethanol content of regulating in the filtrate is 5wt%, obtains containing plant class general flavone ethanol solution.
2, adding glucose 30g, sodium hydrogen phosphate 3g, potassium dihydrogen phosphate 3g, yeast extract 2g, peptone 4g are mixed with fermentation medium in the plant class general flavone ethanol solution of every 1L.
3, choose the activation of the energy cellulosic rhizobium of secreting bacteria and Rhodopseudomonas and be prepared into the seed mash, the even access of seed mash that with bacterial strain concentration is 60wt% then contained in the fermentation medium of plant class general flavone ethanol solution, takes out tunning behind the fermentation culture 6d.
4, tunning heats 6h under 60 ℃ temperature in the quality percentage composition is 4% NaOH aqueous solution.Wash to neutrality repeatedly with distilled water again.To remove tropina and the residual media that sticks on the cellulose membrane.Obtain having the Bacterial cellulose facial film of radioprotective effect after the cutting, Bacterial cellulose three-dimensional network inside is evenly distributed with the total flavones molecule, and the molecule of Bacterial cellulose and the intermolecular bonding action that exists of total flavones.
Below further specify beneficial effect of the present invention by test
Test 1
The skin of face drying is selected in each processing respectively, tangible patient's 120 examples of casting off a skin are divided into 6 groups, every group of 20 people test, get two of the facial films of embodiment 1-6 gained, respectively at early morning with each spreads on face after washing one's face with warm boiled water just before going to bed, keeping about 15min on the face then.Test and add up recovery from illness, produce effects, improvement, invalid sufferer number after 30 days, calculate its total effective rate (%).Curative effect determinate standard: 1. recovery from illness: the colour of skin is improved, is casted off a skin and disappear fully, and skin smooth moistens; 2. produce effects: the colour of skin has largely to be improved, casts off a skin and obviously disappear; 3. take a turn for the better: the colour of skin makes moderate progress, casts off a skin and disappear to some extent; 4. invalid: the colour of skin, casting off a skin changes not obvious or increases the weight of.Result of the test sees Table 1.
The xerosis cutis of table 1 embodiment 1-6, patient's result of use table of casting off a skin
Test 2
Choose 60 volunteers and test, 60 volunteers are divided into into 6 groups, every group of 10 people.Test divides to be carried out in two days, the method of test in first day is that every volunteer is using pure water facial film (non-woven fabrics facial film, the liquid that adds is water) precedingly earlier test forehead respectively with Fes Kai Er SK-III type hand-held digital display skin moisture tester, left side face, the moisture of right face and chin skin, and get arithmetic mean of instantaneous value, apply facial film of the present invention after cleaning face then, take off facial film after 15 minutes, wash one's face with clear water, dry face, test forehead with the skin moisture tester again after 1 hour, left side face, the skin moisture content of right face and the same position of chin, and get arithmetic mean of instantaneous value; The method of test in second day is identical with first day method of testing, but used facial film is facial film of the present invention, first group tests this experimental example 1 product, second group tests this experimental example 2 products, the 3rd group tests this experimental example 3 products, the 4th group tests this experimental example 4 products, and the 5th group tests this experimental example 5 products, and the 6th group tests this experimental example 6 products.Every group of 10 volunteers get arithmetic mean of instantaneous value with the result who tests, test result such as table 2:
Table 2
After six groups of volunteers used pure water facial film and embodiment 1～6 prepared product to compare respectively, embodiment 1～6 prepared product all can obviously improve the facial skin moisture.
Claims (7)
1. preparation method with Bacterial cellulose facial film of radioprotective effect, it is characterized in that: choose and the cellulosic bacterial strain activation of secreting bacteria to be prepared into the seed mash, the even access of seed mash that with bacterial strain concentration is 30～90wt% then contained in the fermentation medium of plant class general flavone ethanol solution, take out tunning behind fermentation culture 3～7d, the purified processing of tunning, cutting, the Bacterial cellulose facial film that obtains having the radioprotective effect.
2. preparation method as claimed in claim 1 is characterized in that: described can the cellulosic bacterial strain of secreting bacteria be in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter one or more.
3. preparation method as claimed in claim 1 is characterized in that: the described plant class general flavone ethanol solution that contains is to be joined in the alcoholic solution and by supersound process by in Radix et Caulis Opuntiae Dillenii, Aloe, Folium Camelliae sinensis, Semen Ginkgo, the Folium Bambusae plant lyophilized powder one or more to obtain.
4. preparation method as claimed in claim 3, it is characterized in that: described supersound process treatment step is:
(1) one or more addings in Radix et Caulis Opuntiae Dillenii, Aloe, Folium Camelliae sinensis, Semen Ginkgo, the Folium Bambusae plant lyophilized powder are accounted in the ethanol water of 20～50 times of plant lyophilized powder weight, wherein ethanol water concentration is 40～80wt%;
(2) with above-mentioned solution ultrasonic 5～40min under the ultrasonic power of 100～800W, remove by filter filtering residue;
(3) add distilled water in filtrate filtered, the ethanol content of regulating in the filtrate is 5～15wt%, obtains containing plant class general flavone ethanol solution.
5. preparation method as claimed in claim 1, it is characterized in that: described fermentation medium is: contain glucose 10～30g, sodium hydrogen phosphate 1～4g, potassium dihydrogen phosphate 1～4g, yeast extract 1～5g, peptone 1～5g in the plant class general flavone ethanol solution of every 1L.
6. preparation method as claimed in claim 1 is characterized in that: described purification treating method is, tunning heats 3～6h under 30～100 ℃ temperature in the quality percentage composition is 1～8% NaOH aqueous solution, and water washes repeatedly to neutrality again.
7. preparation method as claimed in claim 1, it is characterized in that: described Bacterial cellulose facial film with radioprotective effect refers to that the Bacterial cellulose three-dimensional network inside that is obtained by strain fermentation is evenly distributed with total flavones.
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| CN105054218A (en) * | 2015-08-30 | 2015-11-18 | 陈爱梅 | Preparation method for food desiccant |
| CN105231252A (en) * | 2015-08-30 | 2016-01-13 | 陈爱梅 | Preparation method of natural food drying agent |
| CN107157780A (en) * | 2017-05-19 | 2017-09-15 | 陕西农产品加工技术研究院 | A kind of preparation method of aloe bacteria cellulose Pure natural mask |
| CN107441316A (en) * | 2017-08-25 | 2017-12-08 | 杨润 | A kind of disinfectant for radiation chamber and preparation method thereof |
| CN107951806A (en) * | 2017-12-19 | 2018-04-24 | 广东真丽斯化妆品有限公司 | A kind of gentle more effect aloe skin care item and its preparation process |
| CN108451791A (en) * | 2018-07-02 | 2018-08-28 | 河南工业大学 | A kind of preparation method of sericin/hyaluronic acid-bacteria cellulose compound bio facial mask |
| CN108703919A (en) * | 2018-08-25 | 2018-10-26 | 郑州成济堂生物科技有限公司 | A kind of preparation method of lasting water conservation biology cellulose facial mask |
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| CN105054218A (en) * | 2015-08-30 | 2015-11-18 | 陈爱梅 | Preparation method for food desiccant |
| CN105231252A (en) * | 2015-08-30 | 2016-01-13 | 陈爱梅 | Preparation method of natural food drying agent |
| CN105054218B (en) * | 2015-08-30 | 2018-11-06 | 陈林 | A kind of preparation method of food drier |
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| CN107157780A (en) * | 2017-05-19 | 2017-09-15 | 陕西农产品加工技术研究院 | A kind of preparation method of aloe bacteria cellulose Pure natural mask |
| CN107441316A (en) * | 2017-08-25 | 2017-12-08 | 杨润 | A kind of disinfectant for radiation chamber and preparation method thereof |
| CN107951806A (en) * | 2017-12-19 | 2018-04-24 | 广东真丽斯化妆品有限公司 | A kind of gentle more effect aloe skin care item and its preparation process |
| CN110403858A (en) * | 2018-04-28 | 2019-11-05 | 南京理工大学 | Bacteria cellulose/Pu'er tea composite functional material and preparation method thereof |
| CN108451791A (en) * | 2018-07-02 | 2018-08-28 | 河南工业大学 | A kind of preparation method of sericin/hyaluronic acid-bacteria cellulose compound bio facial mask |
| CN108703919A (en) * | 2018-08-25 | 2018-10-26 | 郑州成济堂生物科技有限公司 | A kind of preparation method of lasting water conservation biology cellulose facial mask |
| CN108703919B (en) * | 2018-08-25 | 2021-04-27 | 素妆生物科技(上海)有限公司 | Preparation method of biological cellulose facial mask capable of keeping water for long time |
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