CN111187209A - Preparation method for extracting nuciferine from lotus leaves - Google Patents

Abstract

The invention discloses a preparation method for extracting nuciferine from lotus leaves, which is characterized by comprising the following steps: 1) grinding dried lotus leaves into coarse powder; 2) taking coarse powder, lg cellulase and 40mL of citric acid buffer solution with pH = 5; 3) performing enzymolysis for 2h in a water bath at 50 ℃; 4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min; 5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times; 6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.

Description

Preparation method for extracting nuciferine from lotus leaves

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of nuciferine.

Background

Lotus leaf is also called lotus flower stem and lotus stem. Is leaf of Nelumbo nucifera Gaertn of Nelumbo of Nelumbonaceae, which is distributed in subtropical and temperate regions of middle Asia, Western Asia, North America, India, China, Japan, etc. China can be cultivated as early as three thousand years, and carbonized ancient lotus seeds are found in Liaoning and Zhejiang nowadays, which has a long history. Whereas taiwan was introduced from japan 100 years ago. Some remote places in Asia still have wild lotus to date, but most lotus is planted artificially to decorate and eat as landscape, for example, lotus leaves are planted as cash crops in China. Many people have mistakenly conceived that the lotus is produced in egyptian and mediterranean zones. In about 500 years before the Gongyuan, the lotus introduced into Egypt, and was probably carried away by Persian. The lotus leaves are bitter, pungent and slightly astringent, and cool in nature and cold in taste, which injure the spleen and stomach, and need to be slightly cold and warm to relieve the cold and cold nature. It has faint scent and ascends and disperses when entering heart, liver and spleen channels; has effects in removing summer-heat, promoting diuresis, invigorating spleen, invigorating yang, removing blood stasis, and stopping bleeding; can be used for treating summer heat, polydipsia, headache, giddiness, edema, anorexia, abdominal distention, dysentery, leucorrhea, rectocele, hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, puerperal lochiorrhea, and injury blood stasis.

Disclosure of Invention

The invention aims to provide a method for extracting nuciferine, which has wide medicinal and health-care values.

The invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting total flavone

1) Grinding dried lotus leaves into coarse powder;

2) taking coarse powder, l g cellulase and 40mL of citric acid buffer solution with pH = 5;

3) performing enzymolysis for 2h in a water bath at 50 ℃;

4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min;

5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times;

6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.

Step 2) the citric acid was 40mL pH = 5.

The temperature of the water bath in step 3) is 50 ℃.

The invention has the advantages that

The invention employs natural destruction of cells with cellulase

And the barrier is used for releasing substances in cells to achieve the aim of extracting effective components, and the experimental conditions are mild. Meanwhile, influence factors of cellulase for extracting nuciferine from lotus leaves are discussed. By qualitative and quantitative analysis of nuciferine in the enzymolysis product, the optimal enzymolysis reaction condition for improving the nuciferine content is preferably selected, so that an effective way is provided for comprehensive utilization of medicinal material resources of lotus.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

The specific implementation mode is as follows:

example 1:

1) grinding dried lotus leaves into coarse powder;

2) taking coarse powder, l g cellulase and 40mL of citric acid buffer solution with pH = 5;

3) performing enzymolysis for 2h in a water bath at 50 ℃;

4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min;

5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times;

6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.

Example 2:

1) grinding dried lotus leaves into coarse powder;

2) taking coarse powder, l g cellulase and 40mL of citric acid buffer solution with pH = 6;

3) performing enzymolysis for 2h in a water bath at 50 ℃; 4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min;

5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times;

6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.

Example 3:

1) grinding dried lotus leaves into coarse powder;

2) taking coarse powder, l g cellulase and 40mL of citric acid buffer solution with pH = 7;

3) performing enzymolysis for 2h in a water bath at 50 ℃;

4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min;

5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times;

6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.

Claims (1)

1. A preparation method for extracting nuciferine from lotus leaves is characterized by comprising the following steps:

1) grinding dried lotus leaves into coarse powder;

2) taking coarse powder, l g cellulase and 40mL of citric acid buffer solution with pH = 5;

3) performing enzymolysis for 2h in a water bath at 50 ℃;

4) then adding 150mL of 0.5% HCl solution, soaking for 16h, and carrying out ultrasonic treatment for 20 min;

5) suction filtering, concentrating the filtrate with rotary evaporator, adjusting pH to 3, centrifuging at 5000 r/min, and extracting the clear liquid with chloroform for 2 times;

6) mixing chloroform solutions, evaporating chloroform to obtain nuciferine, and dissolving with anhydrous ethanol.