CN104606262A - Method for extracting total flavones from passiflora edulis peels - Google Patents
Method for extracting total flavones from passiflora edulis peels Download PDFInfo
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- CN104606262A CN104606262A CN201410818323.1A CN201410818323A CN104606262A CN 104606262 A CN104606262 A CN 104606262A CN 201410818323 A CN201410818323 A CN 201410818323A CN 104606262 A CN104606262 A CN 104606262A
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- total flavones
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Abstract
The invention relates to the processing field of natural medicine, in particular to a method for extracting total flavones from passiflora edulis peels. The method comprises the following steps: (1) carrying out raw material pretreatment: drying the passiflora edulis peels in the sun, and crushing; (2) adding an ethanol solvent to soak for a certain period of time; (3) carrying out ultrasonic-assisted extraction to obtain a mixture; (4) carrying out suction filtration to obtain a filter residue and a filter liquor containing total flavones and ethyl alcohol; (5) heating and evaporating to remove ethyl alcohol and water so as to obtain crude flavone particles; (6) obtaining high-purity total flavones through purification. The method is used for turning waste into wealth by using the passiflora edulis peels, so that the peels realize medicinal value; the method disclosed by the invention has the advantages of simple technology, simple and convenient operation, small amount of used solvent, low cost, high extraction ratio of total flavones, high purity of total flavones obtained through purification, and the like.
Description
Technical field
The present invention relates to natural drug manufacture field, especially a kind of method extracting total flavones from passion flower-fruit peel.
Background technology
(passion fruit has another name called Passifolra edulis to Herba Passiflorae Caeruleae, Passiflora edulis sims), Herba Passiflorae Caeruleae has strong unique perfume because the fragrance component being permitted various fruits containing Fructus psidii guajavae immaturus, Fructus Mangifera Indicae, Fructus Mali pumilae, Fructus Ananadis comosi etc. is described as " Passifolra edulis ", acidity is higher, passion flower fruit contains the aromatic substance more than more than 132 kinds, be one of the most fragrant known fruit in the world, have the good reputation of " king of fruit juice ".Herba Passiflorae Caeruleae is rich in multivitamin, mineral element, the essential amino acids such as V C and has alkaloid and the Flavonoid substances of medical value.Normal edible Herba Passiflorae Caeruleae has refreshment, skin maintenance, promoting the production of body fluid to quench thirst, helps digest, preventing phlegm from forming and stopping coughing, constipation relieving, invigorate blood circulation and keep fit, improve immune function of human body, enriching yin and nourishing kidney, allaying tiredness, hypertension and hyperlipemia, slow down aging, antihypertensive effect.The root of Herba Passiflorae Caeruleae, stem, leaf have the effect such as anti-inflammatory analgetic, blood fat-reducing blood pressure-decreasing.
Flavone compound is a kind of general name with the phenolic compound of the structure of C6-C3-C6, all detect in the plants such as Semen Ginkgo, Fructus Lycii, Fructus Canarii albi, Flos Lonicerae, Folium Camelliae sinensis, Fructus Crataegi, it is one of medicinal main component, there is the physiologically actives such as Adjust-blood lipid, antioxidation, antitumor, antiviral, play an important role to disease such as treatment cardiovascular, cancer etc., therefore it by domestic and international extensive concern.
The sarcocarp of Herba Passiflorae Caeruleae can cook beverage or directly use, and the peel that Herba Passiflorae Caeruleae is left much just directly works as offal treatment, but, containing a large amount of flavone compounds in passion flower-fruit peel.The present invention will provide a kind of method extracting total flavones from passion flower-fruit peel.
Summary of the invention
The object of the invention is to provide a kind of method extracting total flavones from passion flower-fruit peel.Flavone compound is one of medicinal main component, there is the physiologically actives such as Adjust-blood lipid, antioxidation, antitumor, antiviral, disease such as treatment cardiovascular, cancer etc. is played an important role, flavone is extracted from passion flower-fruit peel, passion flower-fruit peel " can be turned waste into wealth ", allow peel have very large medical value.
For realizing above object, the technical solution used in the present invention is:
Extract a method for total flavones from passion flower-fruit peel, the method comprises the following steps successively:
(1) pretreatment of raw material: after being cleaned up by passion flower-fruit peel, dries at 80-100 DEG C of temperature, makes its water content lower than 10%, is crushed to 5-20 order, obtain Herba Passiflorae Caeruleae powder;
(2) solvent soaking is added: above-mentioned Herba Passiflorae Caeruleae powder and alcohol solvent are soaked 1.5-2h for 1:20-30 joins in port grinding bottle in mass ratio;
(3) ultrasound-assisted extraction: above-mentioned port grinding bottle is put into ultrasound wave, extracts 70-90min, obtains mixture at 50-65 DEG C of water bath with thermostatic control temperature;
(4) sucking filtration: mixture is placed in Suction filtration device and carries out vacuum filtration, obtains filtering residue and the filtrate containing ethanol and flavone;
(5) heating evaporation: by above-mentioned filtrate, heating evaporation second alcohol and water, obtains crude flavonoid powder granule;
(6) purify: by above-mentioned crude flavonoid powder granule, add distilled water 1: 15-25, continue 4-5h 60-80 DEG C of water bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtain flavone granule.
Further, described concentration of alcohol is 50%-70%.
Further, described step (4) needs to clean filtering residue 3-5 time with the mixed liquor of second alcohol and water.
Further, the extraction ratio of passion flower-fruit peel total flavones is 6.5%-7.2%.
The beneficial effect that the present invention obtains:
(1) the present invention extracts total flavones from passion flower-fruit peel, and the passion flower-fruit peel raw material of employing is easy to get, and cost is low, and " being turned waste into wealth " by passion flower-fruit peel realizes its medical value; Filtrate after extraction and filtering residue also can be used for extracting pectin, make full use of resource.
(2) the present invention has the advantages such as technique is simple, simple to operate, pollution-free.
(3) in the present invention, the extraction ratio of passion flower-fruit peel total flavones is 6.5%-7.2%, and the purity obtaining flavone through purifying is high.
Accompanying drawing explanation
Fig. 1 is the process chart extracting total flavones from passion flower-fruit peel.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but protection scope of the present invention is not limited only to embodiment.
embodiment 1
Extract a method for total flavones from passion flower-fruit peel, comprise the following steps successively:
(1) pretreatment of raw material:
Take 500g passion flower-fruit peel, first with tap water, passion flower-fruit peel is cleaned up with after washing water of rice, dry at 80 DEG C of temperature, make its water content lower than 10%, be crushed to 10 orders, obtain Herba Passiflorae Caeruleae powder.
(2) solvent soaking is added:
By on Herba Passiflorae Caeruleae powder and 60% alcohol solvent are soaked 1.5h for 1:25 joins in port grinding bottle in mass ratio.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 60 DEG C of water bath with thermostatic control temperature, carries 90min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 3 times with the mixed liquor of 60% second alcohol and water 1:1 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation second alcohol and water, obtains 38.28g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:15, continue 4h 60 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 33.55g total flavones granule.
embodiment 2
Extract a method for total flavones from passion flower-fruit peel, comprise the following steps successively:
(1) pretreatment of raw material:
Take 500g passion flower-fruit peel, first with tap water, passion flower-fruit peel is cleaned up with after washing water of rice, dry at 100 DEG C of temperature, make its water content lower than 10%, be crushed to 5 orders, obtain Herba Passiflorae Caeruleae powder.
(2) solvent soaking is added:
By on Herba Passiflorae Caeruleae powder and 65% alcohol solvent are soaked 2h for 1:30 joins in port grinding bottle in mass ratio.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 65 DEG C of water bath with thermostatic control temperature, extracts 70min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 4 times with the mixed liquor of 65% second alcohol and water 1:1 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation second alcohol and water, obtains 39.34g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:20, continue 4h 60 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 34.93g total flavones granule.
embodiment 3
Extract a method for total flavones from passion flower-fruit peel, comprise the following steps successively:
(1) pretreatment of raw material:
Take 500g passion flower-fruit peel, first with tap water, passion flower-fruit peel is cleaned up with after washing water of rice, dry at 80 DEG C of temperature, make its water content lower than 10%, be crushed to 20 orders, obtain Herba Passiflorae Caeruleae powder.
(2) solvent soaking is added:
By on Herba Passiflorae Caeruleae powder and 50% alcohol solvent are soaked 2h for 1:20 joins in port grinding bottle in mass ratio.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 50 DEG C of water bath with thermostatic control temperature, carries 80min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 5 times with the mixed liquor of 50% second alcohol and water 1:1 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation second alcohol and water, obtains 37.94g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:20, continue 4h 60 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 32.87g total flavones granule.
Claims (4)
1. extract a method for total flavones from passion flower-fruit peel, it is characterized in that: the method comprises the following steps successively:
(1) pretreatment of raw material: cleaned up by passion flower-fruit peel, dries at 80-100 DEG C of temperature, makes its water content lower than 10%, is crushed to 5-20 order, obtain Herba Passiflorae Caeruleae powder;
(2) solvent soaking is added: above-mentioned Herba Passiflorae Caeruleae powder and alcohol solvent are soaked 1.5-2h for 1:20-30 joins in port grinding bottle in mass ratio;
(3) ultrasound-assisted extraction: above-mentioned port grinding bottle is put into ultrasound wave, extracts 70-90min, obtains mixture at 50-65 DEG C of water bath with thermostatic control temperature;
(4) sucking filtration: mixture is placed in Suction filtration device and carries out vacuum filtration, obtains filtering residue and the filtrate containing ethanol and flavone;
(5) heating evaporation: by above-mentioned filtrate, heating evaporation second alcohol and water, obtains crude flavonoid powder granule;
(6) purify: by above-mentioned crude flavonoid powder granule, add distilled water 1: 15-25, continue 4-5h 60-80 DEG C of water bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtain flavone granule.
2. a kind ofly according to claim 1 extract the method for total flavones from passion flower-fruit peel, it is characterized in that: described concentration of alcohol is 50%-65%.
3. a kind ofly according to claim 1 extract the method for total flavones from passion flower-fruit peel, it is characterized in that: described step (4) needs mixed liquor cleaning filtering residue 3-5 time with second alcohol and water.
4., according to the arbitrary a kind of described method extracting total flavones from passion flower-fruit peel of claim 1-3, it is characterized in that: in passion flower-fruit peel, the extraction ratio of total flavones is 6.5%-7.2%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660844A (en) * | 2016-03-09 | 2016-06-15 | 广西鹿寨县绿享科技有限责任公司 | Passion fruit fresh-keeping agent and preparation method thereof |
| CN105767133A (en) * | 2016-03-09 | 2016-07-20 | 广西鹿寨县绿享科技有限责任公司 | Freshness preservation method of passion fruits |
| CN105815438A (en) * | 2016-03-09 | 2016-08-03 | 广西鹿寨县绿享科技有限责任公司 | Litchi fresh-keeping agent and preparation method thereof |
| CN107897923A (en) * | 2017-12-02 | 2018-04-13 | 桂林理工大学 | A kind of preparation method with flavones in functional passion fruit shell |
| CN108014051A (en) * | 2018-01-10 | 2018-05-11 | 福建师范大学 | A kind of eye essence frost containing Caulis Sacchari sinensis extract and preparation method thereof |
| CN109966327A (en) * | 2019-04-19 | 2019-07-05 | 华南农业大学 | A kind of method of the double assisted extraction passionflower seed oil meal general flavones of ultrasonic wave, microwave |
Citations (1)
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| CN102285949A (en) * | 2011-08-02 | 2011-12-21 | 桂林三宝药业有限公司 | Method for extracting chrysin from passiflora edulis fruit residue obtained after juicing |
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2014
- 2014-12-24 CN CN201410818323.1A patent/CN104606262A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102285949A (en) * | 2011-08-02 | 2011-12-21 | 桂林三宝药业有限公司 | Method for extracting chrysin from passiflora edulis fruit residue obtained after juicing |
Non-Patent Citations (1)
| Title |
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| 冯纪南等: "西番莲果皮总黄酮提取工艺及抑制亚硝化反应", 《光谱实验室》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660844A (en) * | 2016-03-09 | 2016-06-15 | 广西鹿寨县绿享科技有限责任公司 | Passion fruit fresh-keeping agent and preparation method thereof |
| CN105767133A (en) * | 2016-03-09 | 2016-07-20 | 广西鹿寨县绿享科技有限责任公司 | Freshness preservation method of passion fruits |
| CN105815438A (en) * | 2016-03-09 | 2016-08-03 | 广西鹿寨县绿享科技有限责任公司 | Litchi fresh-keeping agent and preparation method thereof |
| CN107897923A (en) * | 2017-12-02 | 2018-04-13 | 桂林理工大学 | A kind of preparation method with flavones in functional passion fruit shell |
| CN108014051A (en) * | 2018-01-10 | 2018-05-11 | 福建师范大学 | A kind of eye essence frost containing Caulis Sacchari sinensis extract and preparation method thereof |
| CN109966327A (en) * | 2019-04-19 | 2019-07-05 | 华南农业大学 | A kind of method of the double assisted extraction passionflower seed oil meal general flavones of ultrasonic wave, microwave |
| CN109966327B (en) * | 2019-04-19 | 2021-08-31 | 华南农业大学 | Method for extracting passion flower seed oil meal total flavonoids by ultrasonic and microwave double-auxiliary extraction |
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