CN104586917A - Method for extracting total flavonoid from dragon fruit peels - Google Patents
Method for extracting total flavonoid from dragon fruit peels Download PDFInfo
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- CN104586917A CN104586917A CN201410817772.4A CN201410817772A CN104586917A CN 104586917 A CN104586917 A CN 104586917A CN 201410817772 A CN201410817772 A CN 201410817772A CN 104586917 A CN104586917 A CN 104586917A
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- water
- dragon fruit
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- total flavones
- pitaya peel
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Abstract
The invention relates to the field of natural medicine extraction, and in particular relates to a method for extracting total flavonoid from dragon fruit peels. The method sequentially comprises the following steps: (1) raw material preprocessing, namely drying the dragon fruit peels in sun and grinding the dragon fruit peels; (2) soaking the ground dragon fruit peels in an ethanol solvent for a certain time; (3) carrying out ultrasonic-assisted extraction so as to obtain a mixture; (4) carrying out suction filtration to obtain filter residue and filtrate containing total flavonoid and ethanol; (5) heating and evaporating to remove the ethanol and water so as to obtain coarse flavonoid particles; and (6) purifying the coarse flavonoid particles so as to obtain the high-purity total flavonoid. By extracting the total flavonoid from the dragon fruit peels, the method can be used for recycling the waste peels, so that the added value of the dragon fruit peels are improved, the income of fruit farmers is increased, the comprehensive utilization of the dragon fruit peels is achieved and the peels have a medicinal value, and the environmental pollution is reduced as well; and meanwhile, the method disclosed by the invention has the advantages of being simple in extraction process, simple and convenient to operate, few in adopted solvents, low in cost, high in total flavonoid extracting rate, high in total flavonoid purity and the like.
Description
Technical field
The present invention relates to natural drug and extract field, especially a kind of method extracting total flavones from pitaya peel.
Background technology
Hylocereus undatus (Pitaya), another name Green Dragon fruit, Hylocereus undatus.Be distributed widely in the ground such as China Hainan, Guangdong, Guangxi.Hylocereus undatus is nutritious, is rich in a large amount of natural pigment, vegetable protein, abundant vitamin, mineral element and water soluble dietary fiber.Because Hylocereus undatus seldom occurs disease and pest, any pesticide and hormone can not be used, belong to environmental protection fruit and there is development space widely.Hylocereus undatus has antioxidation, free radical resisting, antidotal effect, also has and suppresses brain cell degeneration, the effect such as prevention dementia, heat-clearing and toxic substances removing, skin whitening, fat-reducing, reduction cholesterol, aid digestion, intestine moistening, prevention colorectal cancer.
Flavone compound is a kind of general name with the phenolic compound of the structure of C6-C3-C6, all detect in the plants such as Semen Ginkgo, Fructus Lycii, Fructus Canarii albi, Flos Lonicerae, Folium Camelliae sinensis, Fructus Crataegi, it is one of medicinal main component, there is the physiologically actives such as Adjust-blood lipid, antioxidation, antitumor, antiviral, disease such as treatment cardiovascular, cancer etc. is played an important role, and because its toxicity is well below synthetized oxidation preventive agent, become the development goal of the Natural antioxidant in food, the Flavonoid substances of current plant origin all has wide in medicine, food and cosmetics etc.
General application.
At present, China mainly concentrates on HUOLONG sarcocarp to the research of Hylocereus undatus, rare relevant Hylocereus undatus peel total flavones research report.The sarcocarp of Hylocereus undatus can directly eat or cook fruit juice, and remaining peel is a lot of just directly works as offal treatment, but, containing a large amount of flavone compounds in pitaya peel.The present invention by extracting total flavones from pitaya peel, will can realize turning waste into wealth, can improve the added value of Hylocereus undatus, realizing the comprehensive utilization of Hylocereus undatus, can reduce environmental pollution again.
Summary of the invention
The object of the invention is to provide a kind of method extracting total flavones from pitaya peel.Flavone compound is one of medicinal main component, has the physiologically actives such as Adjust-blood lipid, antioxidation, antitumor, antiviral, plays an important role to disease such as treatment cardiovascular, cancer etc.By extracting total flavones from pitaya peel, can realize turns waste into wealth improves the added value of Hylocereus undatus, allows peel have medical value, realizes the comprehensive utilization of pitaya peel, can reduce environmental pollution again.
For realizing above object, the technical solution used in the present invention is:
Extract a method for total flavones from pitaya peel, the method comprises the following steps successively:
(1) pretreatment of raw material: cleaned up by pitaya peel, dries at 100-120 DEG C of temperature, makes its water content≤15%, is crushed to 10-30 order, obtains powder peel;
(2) solvent soaking is added: above-mentioned powder peel and alcohol solvent are soaked 2-3h for 1:15-25 joins in port grinding bottle in mass ratio;
(3) ultrasound-assisted extraction: above-mentioned port grinding bottle is put into ultrasound wave, extracts, obtains mixture at 45-60 DEG C of water bath with thermostatic control temperature;
(4) sucking filtration: mixture is placed in Suction filtration device and carries out vacuum filtration, obtains filtering residue and the filtrate containing ethanol and flavone;
(5) heating evaporation: by above-mentioned filtrate, heating evaporation second alcohol and water, obtains crude flavonoid powder granule;
(6) purify: by above-mentioned crude flavonoid powder granule, add distilled water 1: 10-20, continue 3-4h 50-65 DEG C of water bath with thermostatic control, flavone component is fully dissolved in water, adopt 100-200 order filter screen filtration, obtain filtrate, heating evaporation moisture, obtain flavone granule.
Further, described concentration of alcohol is 60%-70%.
Further, the extraction time of described step (3) is 100-120min.
Further, described step (4) needs to clean filtering residue 3-5 time with the mixed liquor of second alcohol and water.
Further, in pitaya peel, the extraction ratio of total flavones is 6.8%-7.4%.
The beneficial effect that the present invention obtains:
(1) the present invention is by extracting total flavones from pitaya peel, is turned waste into wealth by peel, can improve the added value of Hylocereus undatus, improves the income of orchard worker, realizes the comprehensive utilization of Hylocereus undatus, allow peel have medical value, can reduce environmental pollution again; Filtrate after extraction and filtering residue also can be used for extracting pectin, and make full use of resource, pitaya peel raw material is easy to get, with low cost.
(2) the present invention has the advantages such as technique is simple, simple to operate, solvent load is few, pollution-free.
(3) in the present invention, the extraction ratio of pitaya peel total flavones is 6.8%-7.4%, and the purity obtaining flavone through purifying is high.
Accompanying drawing explanation
Fig. 1 is the process schematic representation extracting total flavones from pitaya peel.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, but protection scope of the present invention is not limited only to embodiment.
embodiment 1
Extract a method for total flavones from pitaya peel, the method comprises the following steps successively:
(1) pretreatment of raw material:
Take 1kg pitaya peel, first with tap water, pitaya peel is cleaned up with after washing water of rice, dry at 100 DEG C of temperature, make water content be 15%, be crushed to 10 orders, obtain powder peel.
(2) solvent soaking is added:
By on be that 60% alcohol solvent soaks 2h for 1:20 joins in port grinding bottle in mass ratio by powder peel and concentration.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 45 DEG C of water bath with thermostatic control temperature, carries 120min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 3 times with the mixed liquor that 60% second alcohol and water is 1:1 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation ethanol and water, obtain 78.54g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:15, continue 4h 55 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 71.24g total flavones granule.
embodiment 2
(1) pretreatment of raw material:
Take 1kg pitaya peel, first with tap water, pitaya peel is cleaned up with after washing water of rice, dry at 110 DEG C of temperature, make its water content be 10%, be crushed to 20 orders, obtain powder peel.
(2) solvent soaking is added:
By on Herba Passiflorae Caeruleae powder and 70% alcohol solvent are soaked 3h for 1:25 joins in port grinding bottle in mass ratio.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 50 DEG C of water bath with thermostatic control temperature, extracts 100min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 3 times with the mixed liquor of 70% second alcohol and water 1:1.5 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation ethanol and water, obtain 79.50g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:20, continue 3h 60 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 200 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 73.49g total flavones granule.
embodiment 3
(1) pretreatment of raw material:
Take 1kg pitaya peel, first with tap water, pitaya peel is cleaned up with after washing water of rice, dry at 100 DEG C of temperature, make its water content be 15%, be crushed to 30 orders, obtain powder peel.
(2) solvent soaking is added:
By on powder peel and 60% alcohol solvent are soaked 2h for 1:15 joins in port grinding bottle in mass ratio.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 50 DEG C of water bath with thermostatic control temperature, carries 100min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 3 times with the mixed liquor of 60% second alcohol and water 1:1 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation ethanol and water, obtain 75.45g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:10, continue 3h 50 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 100 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 68.76 total flavones granules.
embodiment 4
Extract a method for total flavones from pitaya peel, the method comprises the following steps successively:
(1) pretreatment of raw material:
Take 1kg pitaya peel, first with tap water, pitaya peel is cleaned up with after washing water of rice, dry at 120 DEG C of temperature, make water content be 15%, be crushed to 30 orders, obtain powder peel.
(2) solvent soaking is added:
By on be that 70% alcohol solvent soaks 2h for 1:25 joins in port grinding bottle in mass ratio by powder peel and concentration.
(3) ultrasound-assisted extraction:
Above-mentioned port grinding bottle is put into ultrasound wave, at 55 DEG C of water bath with thermostatic control temperature, extracts 120min, obtain mixture.
(4) sucking filtration:
Mixture is placed in Suction filtration device and carries out vacuum filtration, obtain filtering residue and the filtrate containing ethanol and flavone, clean filtering residue 4 times with the mixed liquor that 70% second alcohol and water is 1:1.5 by volume.
(5) heating evaporation:
By above-mentioned filtrate, heating evaporation ethanol and water, obtain 76.48g crude flavonoid powder granule.
(6) purify:
By above-mentioned crude flavonoid powder granule, add the distilled water that mass ratio is 1:15, continue 3h 65 DEG C of waters bath with thermostatic control, flavone component is fully dissolved in water, adopts 200 order filter screen filtration, obtains filtrate, heating evaporation moisture, obtains 70.09g total flavones granule.
Claims (5)
1. extract a method for total flavones from pitaya peel, it is characterized in that: the method comprises the following steps successively:
(1) pretreatment of raw material: cleaned up by pitaya peel, dries at 100-120 DEG C of temperature, makes its water content≤15%, is crushed to 10-30 order, obtains powder peel;
(2) solvent soaking is added: above-mentioned powder peel and alcohol solvent are soaked 2-3h for 1:15-25 joins in port grinding bottle in mass ratio;
(3) ultrasound-assisted extraction: above-mentioned port grinding bottle is put into ultrasound wave, extracts, obtains mixture at 45-60 DEG C of water bath with thermostatic control temperature;
(4) sucking filtration: mixture is placed in Suction filtration device and carries out vacuum filtration, obtains filtering residue and the filtrate containing ethanol and flavone;
(5) heating evaporation: by above-mentioned filtrate, heating evaporation second alcohol and water, obtains crude flavonoid powder granule;
(6) purify: by above-mentioned crude flavonoid powder granule, add distilled water 1: 10-20, continue 3-4h 50-65 DEG C of water bath with thermostatic control, flavone component is fully dissolved in water, adopt 100-200 order filter screen filtration, obtain filtrate, heating evaporation moisture, obtain flavone granule.
2. a kind ofly according to claim 1 extract the method for total flavones from pitaya peel, it is characterized in that: described concentration of alcohol is 60%-70%.
3. a kind ofly according to claim 2 extract the method for total flavones from pitaya peel, it is characterized in that: the extraction time of described step (3) is 100-120min.
4. a kind ofly according to claim 3 extract the method for total flavones from pitaya peel, it is characterized in that: described step (4) needs mixed liquor cleaning filtering residue 3-5 time with second alcohol and water.
5., according to the arbitrary a kind of described method extracting total flavones from pitaya peel of claim 1-4, it is characterized in that: in pitaya peel, the extraction ratio of total flavones is 6.8%-7.4%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106265446A (en) * | 2016-09-13 | 2017-01-04 | 广西顺帆投资有限公司 | Whitening moisturizing face masque and preparation method thereof |
| CN106913606A (en) * | 2017-04-01 | 2017-07-04 | 海南大学 | A kind of extracting method of dragon fruit pericarp general flavone |
| CN110151811A (en) * | 2019-05-27 | 2019-08-23 | 嘉兴市爵拓科技有限公司 | A kind of composition and application thereof containing flue berry extract |
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| CN103860921A (en) * | 2012-12-11 | 2014-06-18 | 镇江市丹徒区南山溪园茶叶专业合作社 | Extraction method of bamboo leaf flavone |
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| CN103860921A (en) * | 2012-12-11 | 2014-06-18 | 镇江市丹徒区南山溪园茶叶专业合作社 | Extraction method of bamboo leaf flavone |
| CN103859391A (en) * | 2012-12-11 | 2014-06-18 | 镇江市丹徒区南山溪园茶叶专业合作社 | Preparation method of natural plant flavone mixed capsules |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265446A (en) * | 2016-09-13 | 2017-01-04 | 广西顺帆投资有限公司 | Whitening moisturizing face masque and preparation method thereof |
| CN106913606A (en) * | 2017-04-01 | 2017-07-04 | 海南大学 | A kind of extracting method of dragon fruit pericarp general flavone |
| CN110151811A (en) * | 2019-05-27 | 2019-08-23 | 嘉兴市爵拓科技有限公司 | A kind of composition and application thereof containing flue berry extract |
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Application publication date: 20150506 |