CN105169094A - Method for extracting and purifying total flavonoids from Indocalamus leaves - Google Patents
Method for extracting and purifying total flavonoids from Indocalamus leaves Download PDFInfo
- Publication number
- CN105169094A CN105169094A CN201510737809.7A CN201510737809A CN105169094A CN 105169094 A CN105169094 A CN 105169094A CN 201510737809 A CN201510737809 A CN 201510737809A CN 105169094 A CN105169094 A CN 105169094A
- Authority
- CN
- China
- Prior art keywords
- total flavones
- indocalami tessellati
- folium indocalami
- isolation
- purification method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention belongs to the technical field of natural product separation, food additives and medicines, and in particular relates to a method for extracting and purifying total flavonoids from Indocalamus leaves. The method comprises the following steps: a, washing, drying and crushing Indocalamus leaves; b, presoaking the crushed Indocalamus leaves for 20-60min by using saturated lime water, adding ethanol aqueous solution, then adjusting the acidity and alkalinity of the system to be nearly neutral, performing heating extracting for more than or equal to 30min by controlling the heating extraction temperature to be 50-70 DEG C, and then performing vacuum suction filtration to obtain total a flavonoid extract; c, performing evaporating concentration on the total flavonoid extract, adsorbing by using macroporous resin, washing with water, and then eluting with ethanol aqueous solution to obtain eluent; and d, performing evaporating concentration on the eluent, and performing freeze drying to obtain the total flavonoids. The method provided by the invention has the beneficial effects that the yield of the total flavonoids is relatively high, the concentration of the ethanol solution used during extraction is relatively low, and the temperature during extraction is relatively low.
Description
Technical field
The invention belongs to Separation of Natural Products, food additive, medical art, be specifically related to a kind of extraction and purification method of Folium indocalami tessellati total flavones.
Background technology
Modern study shows, total flavones has the multiple biological activitys such as antitumor, antiinflammatory, antibacterial, atherosclerosis, blood fat reducing, immunomodulating, scavenging free radicals, has significant inhibitory action especially for the generation of tumor, propagation, migration, angiogenesis and multidrug resistance etc.In addition, the normal cell low toxicity of total flavones to human body is even nontoxic, and therefore total flavones has broad application prospects in human health care, nutrition and disease prevention and treatment.
Folium indocalami tessellati is the general name of grass family Bambusoideae Indocalamus Nakai leaves of plants, and this platymiscium is about more than 30 kinds, all originates from China, is exactly the packing material of Dragon Boat Festival mark food-rice tamale since ancient times.Modern pharmacology analysis finds, Folium indocalami tessellati has sterilization, the multiple remarkable effect such as anticorrosion, anticancer, can be widely used in medicine, food and cosmetics of everyday use etc.On the south China the Changjiang river, each provinces and regions all have the wild Folium indocalami tessellati resource exception of a large amount of wild Folium indocalami tessellati resources, particularly Wuling mountain area, Hubei to enrich, but only a small amount of as food packaging article use, and greatly, processing scientific and technological content is not high in the wasting of resources.Research proves, total flavones is the important activity composition of Folium indocalami tessellati, is therefore necessary research Folium indocalami tessellati total flavones being carried out to extraction purification, to improve the comprehensive utilization value of Folium indocalami tessellati.
The method of existing extraction total flavones has circumfluence method, supercritical ultrasonics technology, microwave method etc.But supercritical ultrasonics technology and microwave method need special instrument, and nonrecurring cost drops into high; Direct circumfluence method need use the concentration of ethanol water higher, during backflow, holding temperature is high, reflux extracting time is long, integrated cost is higher, (in document " research of the extracting method of Folium indocalami tessellati total flavones ", the research of Li Shuifang etc. shows to use the alcoholic solution of 85% 3h that refluxes at 85 DEG C to extract Folium indocalami tessellati total flavones; Document " Indocalanmus tessellatus woods architectural feature and the research of Folium Bambusae bioactive ingredients ", the condition extracting total flavones in the research of Su Chunhua etc. is: use concentration of alcohol 85%, solid-liquid ratio 1:20 (g:mL), reflux temperature is 85 DEG C, and extraction time is 50min); Therefore develop a kind of efficient, cheap total flavones extraction and purification technology and have good market prospect.
Summary of the invention
The object of the present invention is to provide a kind of method of Isolation and purification total flavones from Folium indocalami tessellati, this method is easy and simple to handle, and extraction ratio is high, with low cost.
To achieve the above object, the present invention adopts following technical measures: a kind of Isolation and purification method of Folium indocalami tessellati total flavones, comprises the following steps: Folium indocalami tessellati is cleaned by a., oven dry, and pulverizes; B. by the Folium indocalami tessellati saturated limewater pre-soaking 20 ~ 60min of above-mentioned pulverizing, the acid-base value adding regulation system after ethanol water is to close to neutral and more than heating extraction 30min, the temperature of heating extraction controls at 50 ~ 70 DEG C, and then decompress filter obtains total flavones extracting solution; C. by above-mentioned total flavones extracting solution evaporation and concentration, through macroporous resin adsorption, after washing, eluent is obtained with ethanol water eluting; D. by eluent evaporation and concentration, after lyophilization, total flavones is obtained.Eluent concentrates on a rotary evaporator, boils off in solvent to precipitation bottle and remains a small amount of feed liquid and can topple over out, after lyophilization, obtain Folium indocalami tessellati extractive of general flavone.
On the basis of technique scheme, the present invention can also do following improvement.
Further, the drying requirement described in step a is dry 3 ~ 7h at 40 ~ 70 DEG C of temperature.
Further, the pulverizing described in step a requires as the Folium indocalami tessellati granule obtained is 12 ~ 50 orders.
Further, the saturated limewater consumption described in step b is V
1mL, the consumption of described Folium indocalami tessellati is Mg, V
1be 2 ~ 10:1 with the ratio of M.
Further, in ethanol water described in step b, the volume fraction of ethanol is 30 ~ 60%, and the consumption of described ethanol water is V
2mL, the consumption of described Folium indocalami tessellati is Mg, V
2be 10 ~ 30:1 with the ratio of M.
Further, add acid-base value to 6.5 ~ 7.0 of regulation system after ethanol water in step b, the heating extraction time is 40 ~ 60min.The non-oxidizing acid such as spirit of vinegar, dilute hydrochloric acid can be added in system during the acid-base value of regulation system.
Further, the requirement of the evaporation and concentration described in step c is the concentrated rear volume of described total flavones extracting solution is condensate precursor long-pending less than 1/3.Total flavones extracting solution is concentrated on a rotary evaporator, boil off solvent, wherein said rotavapor under vacuum degree is about 0.08MPa, and bath temperature is 45 ~ 55 DEG C, be concentrated into residue feed liquid to stop when being less than 1/3 of original volume, obtain the concentrated solution of Folium indocalami tessellati total flavones extracting solution.
Further, adsorption flow rate during macroporous resin adsorption described in step c is 2 ~ 5BV/h.
Further, in the ethanol water described in step c, the volume fraction of ethanol is 50 ~ 80%, and during eluting, its consumption is 3 ~ 6BV, and elution flow rate is 2 ~ 5BV/h.
Further, the macroporous resin described in step c is any one in HPD800, HPD600, HPD450 and AB-8.
Beneficial effect of the present invention is: the Folium indocalami tessellati that (1) the present invention adopts can be discarded, new fresh Folium indocalami tessellati lobule, damage leaf and the Folium indocalami tessellati waste residue etc. after processing plucked; (2) adopt saturated limewater pre-soaking, in extracting solution, total flavones yield can be increased to 35% than the result of non-pre-soaking, and reach 2.98%, extraction ratio is high; (3) the ethanol contend percent adding ethanol water when extracting is 30 ~ 60%, temperature is 50 ~ 70 DEG C, concentration of alcohol used in the Folium indocalami tessellati total flavone extracting method of comparatively existing bibliographical information is low, Extracting temperature is low, and the impurity such as chlorophyll are few, and cost depletion is low; (4) macroporous resin can regenerate, and reuses, and evaporation and concentration gained ethanol also can reuse; (5) total flavones after extraction purification is not containing objectionable impurities and biological activity is better, can be used for the exploitation of medicine, food etc.
Detailed description of the invention
Be described feature of the present invention below, example, only for explaining the present invention, is not intended to limit scope of the present invention.
In order to understand the present invention better, be illustrated below in conjunction with specific embodiment.
Embodiment 1
Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, add 5 times amount (mL:g, multiple proportion between the numerical value when Folium indocalami tessellati quality of the numerical value when liquor capacity namely added is in units of mL and use is in units of g, multiple proportion in this patent is all like this) saturated limewater solution pre-soaking 0.5h, then add the ethanol water of the volume fraction 50% of 20 times, regulation system pH is about 6.5 ~ 7, at 60 DEG C, extract 40min, decompress filter, obtains total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of HPD800 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent concentrating under reduced pressure, concentrated solution vacuum lyophilization, total flavones yield is 2.98%, finds that its biological activity keeps better through coherent detection.
Embodiment 2:
Similar to Example 1, but do not add saturated limewater pre-soaking.Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the ethanol water of the volume fraction 50% of 20 times, at 60 DEG C, extract 40min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of HPD800 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collection eluent concentrates, and concentrated solution vacuum lyophilization, total flavones yield is 1.89%.
Embodiment 3
Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the saturated limewater solution pre-soaking 1h of 2 times amount.Then add the ethanol water of the volume fraction 50% of 20 times, regulation system pH is about 6.5 ~ 7, extracts 40min, decompress filter at 70 DEG C, obtains total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of HPD800 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent concentrating under reduced pressure, concentrated solution vacuum lyophilization, total flavones yield is 2.31%, finds that its biological activity keeps better through coherent detection.
Embodiment 4
Similar to Example 3, but do not add saturated limewater pre-soaking.Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the ethanol water of the volume fraction 50% of 20 times, at 70 DEG C, extract 40min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of HPD800 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent concentrating under reduced pressure, concentrated solution vacuum lyophilization, total flavones yield is 1.95%.
Embodiment 5
Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the saturated limewater solution pre-soaking 0.5h of 5 times amount.Then add the ethanol water of the volume integral mark 30% of 20 times, regulation system pH is about 6.5 ~ 7, extracts 40min, decompress filter at 50 DEG C, obtains total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add AB-8 resin column absorption, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent and concentrate, concentrated solution vacuum lyophilization, total flavones yield is 1.78%, finds that its biological activity keeps better through coherent detection.
Embodiment 6
Similar to Example 5, but do not add saturated limewater pre-soaking.Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, then adds the ethanol water of the volume fraction 30% of 20 times, at 50 DEG C, extract 40min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of AB-8 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collection eluent concentrates, and concentrated solution vacuum lyophilization, total flavones yield is 1.09%.
Embodiment 7:
Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the saturated limewater solution pre-soaking 0.5h of 5 times amount.Then add the ethanol water of the volume fraction 60% of 20 times, at 60 DEG C, extract 60min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add HPD450 resin column absorption, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent and concentrate, concentrated solution vacuum lyophilization, total flavones yield is 2.56%, finds that its biological activity keeps better through coherent detection.
Embodiment 8
Similar to Example 7, but do not add saturated limewater pre-soaking.Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the ethanol water of the volume fraction 60% of 20 times, at 60 DEG C, extract 60min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add the absorption of HPD450 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collection eluent concentrates, and concentrated solution vacuum lyophilization, total flavones yield is 1.96%.
Embodiment 9
Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, adds the saturated limewater solution pre-soaking 20min of 10 times amount.Then add the ethanol water of the volume fraction 60% of 20 times, regulation system pH is about 6.5 ~ 7.At 50 DEG C, extract 40min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is 1/3 of original volume, add HPD600 resin column absorption, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 70%, collect eluent and concentrate, concentrated solution vacuum lyophilization, total flavones yield is 2.48%, finds that its biological activity keeps better through coherent detection.
Embodiment 10
Similar to Example 9, but do not add saturated limewater pre-soaking.Folium indocalami tessellati raw material dries 5h after cleaning at 40 DEG C, adds Chinese medicine grinder, crosses 20 mesh sieves and obtain Folium indocalami tessellati granule after evenly pulverizing.Folium indocalami tessellati granule is put into extraction flask, then adds the ethanol water of the volume fraction 60% of 20 times, at 50 DEG C, extract 40min, decompress filter, obtain total flavones extracting solution.By above-mentioned total flavones extracting solution concentrating under reduced pressure (vacuum 0.095Mpa on a rotary evaporator, temperature 50 C) to precipitation bottle, remain stopping when feed liquid is separated out without material, add the absorption of HPD600 resin column, loading flow velocity 2BV/h, adsorb saturated after, after 5BV column volume water elution, with the ethanol water eluting of volume fraction 50%, collect eluent concentrating under reduced pressure, concentrated solution vacuum lyophilization, total flavones yield is 1.80%.
From above embodiment, Folium indocalami tessellati total flavones provided by the invention extracts and purification process, after adopting saturated limewater pre-soaking, the yield of total flavones significantly improves, the volume fraction lower (30% ~ 60%) of ethanol in the ethanol water used, the temperature of extraction also lower (50 ~ 70 DEG C).
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. an Isolation and purification method for Folium indocalami tessellati total flavones, is characterized in that, comprise the following steps:
A. Folium indocalami tessellati cleaned, dry, and pulverize;
B. by the Folium indocalami tessellati saturated limewater pre-soaking 20 ~ 60min of above-mentioned pulverizing, the acid-base value adding regulation system after ethanol water is to close to neutral and more than heating extraction 30min, the temperature of heating extraction controls at 50 ~ 70 DEG C, and then decompress filter obtains total flavones extracting solution;
C. by above-mentioned total flavones extracting solution evaporation and concentration, through macroporous resin adsorption, after washing, eluent is obtained with ethanol water eluting;
D. by eluent evaporation and concentration, after lyophilization, total flavones is obtained.
2. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, the drying requirement described in step a is dry 3 ~ 7h at 40 ~ 70 DEG C of temperature.
3. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, the pulverizing described in step a requires as the Folium indocalami tessellati granule obtained is 12 ~ 50 orders.
4. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, the saturated limewater consumption described in step b is V
1mL, the consumption of described Folium indocalami tessellati is Mg, V
1be 2 ~ 10:1 with the ratio of M.
5. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, in ethanol water described in step b, the volume fraction of ethanol is 30 ~ 60%, and the consumption of described ethanol water is V
2mL, the consumption of described Folium indocalami tessellati is Mg, V
2be 10 ~ 30:1 with the ratio of M.
6. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, add acid-base value to 6.5 ~ 7.0 of regulation system after ethanol water in step b, the heating extraction time is 40 ~ 60min.
7. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, the requirement of the evaporation and concentration described in step c is the concentrated rear volume of described total flavones extracting solution is condensate precursor long-pending less than 1/3.
8. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, adsorption flow rate during macroporous resin adsorption described in step c is 2 ~ 5BV/h.
9. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to claim 1, is characterized in that, in the ethanol water described in step c, the volume fraction of ethanol is 50 ~ 80%, and during eluting, its consumption is 3 ~ 6BV, and elution flow rate is 2 ~ 5BV/h.
10. the Isolation and purification method of a kind of Folium indocalami tessellati total flavones according to any one of claim 1 to 9, is characterized in that, the macroporous resin described in step c is any one in HPD800, HPD600, HPD450 and AB-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737809.7A CN105169094B (en) | 2015-11-03 | 2015-11-03 | Indocalamus leaf total flavone extracting and purifying method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737809.7A CN105169094B (en) | 2015-11-03 | 2015-11-03 | Indocalamus leaf total flavone extracting and purifying method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105169094A true CN105169094A (en) | 2015-12-23 |
| CN105169094B CN105169094B (en) | 2019-12-27 |
Family
ID=54891842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510737809.7A Expired - Fee Related CN105169094B (en) | 2015-11-03 | 2015-11-03 | Indocalamus leaf total flavone extracting and purifying method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105169094B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769943A (en) * | 2016-03-04 | 2016-07-20 | 陈爱华 | Method for extracting moringa oleifera flavone from moringa oleifera leaves and moringa oleifera healthcare product |
| CN105920415A (en) * | 2016-06-20 | 2016-09-07 | 沈丹虹 | Response surface methodology chequer-shaped indocalamus leaf general flavones ultrasonic extraction method |
| CN105998792A (en) * | 2016-06-20 | 2016-10-12 | 嘉兴职业技术学院 | Method for extracting general flavones from chequer-shaped indocalamus leaves |
| CN109259233A (en) * | 2018-11-14 | 2019-01-25 | 张家界康华实业有限公司 | A kind of fresh cilantro natural component extraction process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648965A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Industrialization preparation method of lophatherum gracile general flavone |
| CN102671017A (en) * | 2012-04-27 | 2012-09-19 | 湖北长友现代农业股份有限公司 | Method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide |
| CN104189492A (en) * | 2014-09-12 | 2014-12-10 | 广西中医药大学 | Traditional Chinese medicine preparation with hypoglycemic effect |
-
2015
- 2015-11-03 CN CN201510737809.7A patent/CN105169094B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648965A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Industrialization preparation method of lophatherum gracile general flavone |
| CN102671017A (en) * | 2012-04-27 | 2012-09-19 | 湖北长友现代农业股份有限公司 | Method for simultaneously preparing indocalamus leaf volatile oil, total flavone and total polysaccharide |
| CN104189492A (en) * | 2014-09-12 | 2014-12-10 | 广西中医药大学 | Traditional Chinese medicine preparation with hypoglycemic effect |
Non-Patent Citations (1)
| Title |
|---|
| 乐薇等: "箬叶总黄酮提取工艺优化及比较", 《北方园艺》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769943A (en) * | 2016-03-04 | 2016-07-20 | 陈爱华 | Method for extracting moringa oleifera flavone from moringa oleifera leaves and moringa oleifera healthcare product |
| CN105920415A (en) * | 2016-06-20 | 2016-09-07 | 沈丹虹 | Response surface methodology chequer-shaped indocalamus leaf general flavones ultrasonic extraction method |
| CN105998792A (en) * | 2016-06-20 | 2016-10-12 | 嘉兴职业技术学院 | Method for extracting general flavones from chequer-shaped indocalamus leaves |
| CN105998792B (en) * | 2016-06-20 | 2019-07-02 | 嘉兴职业技术学院 | A method of extracting general flavone from indocalamus leaf |
| CN109259233A (en) * | 2018-11-14 | 2019-01-25 | 张家界康华实业有限公司 | A kind of fresh cilantro natural component extraction process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105169094B (en) | 2019-12-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101336949B (en) | Method for extracting polysaccharide and flavone from Gynura divaricata | |
| CN100590109C (en) | Technique for extracting cynamorlum herba cistanches medicinal materials effective component | |
| CN105169094A (en) | Method for extracting and purifying total flavonoids from Indocalamus leaves | |
| CN112870236B (en) | Flavone effective part of abelmoschus manihot and preparation method and application thereof | |
| CN103788218A (en) | Decomposition and extraction method of active ingredients in purple sweet potatoes | |
| CN103387620A (en) | Polysaccharide, total flavonoid and total alksloid prepared from lotus plumule and preparation method thereof | |
| CN105616465A (en) | Method for extracting polyphenol from mango skins | |
| CN104606262A (en) | Method for extracting total flavones from passiflora edulis peels | |
| CN101322737B (en) | Persimmon leaf flavones extract and preparation thereof | |
| CN102178730B (en) | Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof | |
| CN102391116B (en) | Method for extracting chlorogenic acid from honeysuckle flower leaves | |
| CN108567836B (en) | Method for extracting and separating flavone and polysaccharide from hawthorn peel residues in combined manner | |
| CN104098634A (en) | Technology for jointly extracting synephrine, hesperidin and PMFs (polymethoxy latednavones) from fructus aurantii immaturus | |
| CN109966327A (en) | A kind of method of the double assisted extraction passionflower seed oil meal general flavones of ultrasonic wave, microwave | |
| CN103893774B (en) | A kind of beta amylase is extracting the application in Radix Notoginseng total arasaponins | |
| CN109223865B (en) | Preparation method of mulberry leaf alkaloid and application of prepared mulberry leaf alkaloid | |
| CN102204950B (en) | Method for extracting flavonoid from liquorice waste residue | |
| CN104306758A (en) | Method for extracting polyphenol from sugar cane bark | |
| CN101584752B (en) | Extraction and purification process for total flavonoids in Anchusa italica Retiz | |
| CN103385915A (en) | Platycarya fruit extract with anti-oxidization function and preparation method of platycarya fruit extract | |
| CN101638404B (en) | High-purity salvianolic acid B and preparation method and application thereof | |
| CN104983924A (en) | Method for purifying and separating tea polyphenol in waste tea leaves of Mengding tea | |
| CN102648965A (en) | Industrialization preparation method of lophatherum gracile general flavone | |
| CN104586917A (en) | Method for extracting total flavonoid from dragon fruit peels | |
| CN109206528A (en) | A kind of fructus lycii functional component high-performance bio extracting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191227 Termination date: 20211103 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |