CN107303303A - Prepare Tongkat Ali active ingredient and combination application - Google Patents
Prepare Tongkat Ali active ingredient and combination application Download PDFInfo
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- CN107303303A CN107303303A CN201610250227.0A CN201610250227A CN107303303A CN 107303303 A CN107303303 A CN 107303303A CN 201610250227 A CN201610250227 A CN 201610250227A CN 107303303 A CN107303303 A CN 107303303A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
Field is extracted the invention belongs to phytochemicals production, and in particular to the brand-new method for preparing Tongkat Ali active ingredient and combination application.Specially：Tongkat Ali is crushed at a certain temperature obtains aqueous extract with aqueous solution adverse current ultrasonic extraction；Aqueous extract macroporous resin adsorption, binding component uses eluent gradient elution, collects target component, and effective constituents A is made；Macroreticular resin outflow part is classified separation using filter membrane, collects target components, active ingredient B is made；Mixing effective constituents A and B, are made combination of active principles according to a certain percentage.This method is separated and UF membrane using temperature controlled ultrasonic extraction with aqueous solution and with macroporous resin adsorption, the bioactivity for remaining active ingredient is simultaneously selective by its ground enrichment, and the brand-new Tongkat Ali combination of active principles for having enhancing sexual function, improving fertility-rate, antifatigue, strengthen immunity of refreshing oneself, increasing physical efficiency, alleviate gout is made in final rational allocation.
Description
Technical field
Field is extracted the invention belongs to phytochemicals production, and in particular to prepare Tongkat Ali effective ingredients in plant and combination application
Method.
Background introduction
Tongkat Ali (Eurycoma longifolia) is Simarubaceae Tongkat Ali platymiscium, and wild bush is grown on equator
It is distributed more widely in countries in Southeast Asia such as Malaysia, Indonesia, Vietnam in the moist sandy soil of original tropical rain forest.It is existing
Generation research shows that Tongkat Ali has very high medical value.The group that Tongkat Ali plant obtains according to various Different treatments
Divide has active anticancer respectively, antimalarial active, improves the effect such as sexual function.In addition, it is in hypoglycemic, antibacterial, treatment
Also there is certain effect in terms of gout, osteoporosis.The chemical composition separated from Tongkat Ali different parts is with containing hardship
Based on the Diterpenes and canthinone Alkaloid of lignin skeleton, such as eurycomaoside, eurycolactone,
Eurycomalactone, eurycomanone (eurycomanone) and pasakbumin-B.In addition, also containing biphenyl lignin, angle
MF59 derivative, active polysaccharide, glycopeptide and several amino acids.At present, found by our research, the east leather that Indonesia introduces
Ali using this method extract obtain combination of active principles have enhancing sexual function, improve fertility-rate, refresh oneself it is antifatigue, enhancing
Immunity, increase physical efficiency, alleviation pain wind action.
The situation that Tongkat Ali plant uses directly as medicine materical crude slice is more universal, but Tongkat Ali brews and there is mouthfeel bitterness, medicine
The problem of effect acts on the low low aspect with utilization ratio of drug.Therefore, research Tongkat Ali active constituent content is high, and preparation technology can
Lean on, medicinal effects are clear and definite, the product of good mouthfeel just becomes particularly significant.Research data shows that Tongkat Ali is extracted and mainly adopted
Extracted with solvent refluxing or high-temperature water extraction process, such as patent CN104757424A is extracted using decocting method；Patent
US20070224300A1 uses extracting in boiling water；" Tongkat Ali is to training rat TESTO content, metabolism and anti-motion
The influence of fatigue capability " article is prepared using boiling water；" separation and identification of Tongkat Ali chemical composition " article is carried using water backflow
Take.Patent CN103408564A extracts Tongkat Ali with organic solvent；" experiment of Eurycoma longifolia extract product antifatigue effect is ground
Study carefully " article use alcohol extracting taking technique.Although both the above mode extracts product amount relatively many, extracting in boiling water and organic molten
Agent extracting mode can make various active ingredient breakdown or the denaturation among plant, reduce drug effectiveness, while energy resource consumption
Many, environmentally friendly difficulty is high, and solvent cost is big, and extraction process harmfulness is strong.
Many results of study show that Tongkat Ali plant has obvious enhancing sexual function, improves fertility-rate, refreshes oneself really
Antifatigue, strengthen immunity, enhancing physical efficiency, alleviation pain wind action, the extract obtained in a variety of different ways generally have
Certain effect of invigorating, but chemical one-component therein is not fully validated all the time.Comprehensive many article reports and
Itself data, it has been found that quassinoids material by representative of eurycomanone and using active polysaccharide, glycopeptide and amino acids as
Main water-soluble bioactive composition is principle active component therein.We confirm that this two parts is effective according to research and practice
Composition is used in conjunction with certain synergistic function.
The content of the invention
The present invention provides with enhancing sexual function, improves fertility-rate, antifatigue, strengthen immunity of refreshing oneself, increase physical efficiency, alleviation
The Tongkat Ali combination of active principles preparation method of gout.Ultrasonic extraction with aqueous solution active ingredient and selection are not used only for this method
Macroreticular resin carries out adsorbing separation and UF membrane, remains the bioactivity of Tongkat Ali effective components in plants and optionally will
It is enriched with.
The Tongkat Ali active ingredient its preparation method that the present invention is referred to is as follows：
With enhancing sexual function, improve fertility-rate, antifatigue, strengthen immunity of refreshing oneself, enhancing physical efficiency, the east leather for alleviating gout
Ali's combination of active principles preparation method, is mixed after Tongkat Ali is crushed with the aqueous solution, at the ultrasonic water extraction of continuous flow upstream
Reason, obtains Tongkat Ali aqueous extract；
A. resin column after macroporous resin adsorption, absorption is directly over after aqueous extract filter centrifugation removal precipitation to wash through eluent
Take off and collect target components, ethanol precipitation goes the removal of impurity, be concentrated and dried, obtain Tongkat Ali group of effective components
A；
B. efflux of the aqueous extract after macroporous resin adsorption, is classified using filter membrane and separated, and collects target components, decompression
Concentration, freeze-drying are spray-dried, and obtain Tongkat Ali group of effective components B；
C. according to a certain percentage, Tongkat Ali group of effective components A and B are mixed, Tongkat Ali combination of active principles is made.
Specially the mass volume ratio of Tongkat Ali and the aqueous solution is 1: 1~30, and mixture continuous ultrasound extracts 5min~5h, is surpassed
Sound Extracting temperature is 10~70 DEG C.After Tongkat Ali ultrasound aqueous extract filter centrifugation, supernatant macroporous resin enrichment, resin
Type preferred HP-20, D101, DM130, AB-8.Macroporous resin column uses one in ethanol, ethyl acetate, water after absorption
Plant or several solvent mixing gradient elutions.The eluent containing quassinoids target component (eurycomanone) is collected, is concentrated under reduced pressure,
Absolute ethyl alcohol precipitates 2 times and goes the removal of impurity, finally obtained Tongkat Ali group of effective components A.Efflux warp after macroporous resin adsorption,
Using membrane filtering method, the component such as separation Tongkat Ali water-soluble active polysaccharide, glycopeptide and amino acid, spraying or freeze-drying, obtain
Obtain Tongkat Ali group of effective components B.Tongkat Ali group of effective components A is proportionally mixed with Tongkat Ali group of effective components B,
Form Tongkat Ali combination of active principles.Multiple components coexist in Tongkat Ali combination of active principles made from this method, characteristic body
Matter quassinoids component content is 1~50%, and the nutrient composition content such as various active polysaccharide, glycopeptide, amino acid is 1~20%.This
Invention prepare Tongkat Ali combination of active principles have enhancing sexual function, improve fertility-rate, antifatigue, strengthen immunity of refreshing oneself,
Strengthen physical efficiency, alleviate pain wind action.
Extracting temperature is controlled between 10~70 DEG C using the new technology of the present invention first, assisting ultrasonic extracting method can
Retain the activity of two class material compositions.The Tongkat Ali combination of active principles technological process simplicity of preparation, production process safety, ring
Protect, effective component extraction rate, yield are high, be adapted to large-scale production.Secondly propose Tongkat Ali different parts active ingredient
The method being used in mixed way prepares Tongkat Ali combination of active principles, strengthens overall drug effectiveness.The Tongkat Ali of production has
Subassembly is imitated into by many-sided experimental verification, with enhancing sexual function, improve fertility-rate, antifatigue, strengthen immunity of refreshing oneself,
Strengthen physical efficiency, alleviate pain wind action, can apply to that male's non-organic sexual function declines, stress is big, easy fatigue
Deng crowd and the elderly of climacteric metancholia.
Embodiment
The present invention is further illustrated in following specific embodiment, this does not limit the scope of the invention.This is not being departed from
Invent in the case of above-mentioned technological thought, according to ordinary skill knowledge and customary means, make various replacements and change,
It should be included within the scope of the invention.
1. preparation method
The 100.0 grams of crushing of Tongkat Ali root are taken, distilled water 2L immersions are added, water temperature is controlled at 50 DEG C.Using ultrasonic extraction device,
The Tongkat Ali of crushing is mixed with water and continuous ultrasound is carried out and extracted 2 hours, then by filtering, centrifugation, obtains Tongkat Ali
Aqueous extract.The aqueous extract of Tongkat Ali is directly using D101 resins circulation absorption to saturation, with ethanol water to absorption
Post is eluted, and collects quassinoids target component, is obtained ethanol water eluent, is concentrated under reduced pressure, and obtains Tongkat Ali crude product 4.4
Gram.Tongkat Ali crude product absolute ethyl alcohol dissolves, and suction filtration removes the precipitation produced, obtains Tongkat Ali active ingredient ethanol solution,
It is concentrated under reduced pressure, further obtains Tongkat Ali group of effective components A.Trickle is filtered using film device after macroporous resin adsorption,
Separated by microfiltration membranes, milipore filter, NF membrane etc., collection NF membrane and milipore filter retention composition, separation active polysaccharide therein,
Glycopeptide and amino acid, it is final to obtain 1.2g Tongkat Ali group of effective components B.According to 2: 1 ratio mixing Tongkat Ali effectively into
A and B is grouped, Tongkat Ali combination of active principles is prepared into.
2. Mouse Acute Toxicity is tested
Give ICR mouse according to intraperitoneal injection mode by Tongkat Ali combination of active principles, experiment obtain approximate lethal dose,
Maximal non-toxic dosage.Male mice approximate lethal dose scope is 100.0-120.0mg/kg, and maximal non-toxic dosage is 80
mg/kg；Female mice approximate lethal dose scope is 180.0-225.0mg/kg, and maximal non-toxic dosage is 80mg/kg.To dead
Die animal to be dissected, visually observe Other Main Internal Organs no abnormality seen pathological change.
ICR mouse are given according to gastric infusion mode by Tongkat Ali combination of active principles, as a result show that 500mg/kg is administered, it is small
Mouse does not find that toxicity is showed.
Claims (9)
1. preparing the method for Tongkat Ali (also known as eastern brother Ali) combination of active principles, its process is：After Tongkat Ali is crushed with
The aqueous solution is mixed, at a certain temperature, by the ultrasonic water extraction process of continuous flow upstream, obtains Tongkat Ali aqueous extract；
A. aqueous extract filter centrifugation is directly over resin column elution after macroporous resin adsorption, absorption after removing precipitation
And target components are collected, ethanol precipitation goes the removal of impurity, and clear liquid is concentrated and dried, and obtains Tongkat Ali active ingredient
Group A；
B. efflux of the aqueous extract after macroporous resin adsorption, is classified using filter membrane and separated, and collects target components, decompression
Concentration, dry, acquisition Tongkat Ali group of effective components B；
C. according to a certain percentage, Tongkat Ali group of effective components A and B are mixed, Tongkat Ali combination of active principles is made.
2. the preparation method of Tongkat Ali combination of active principles according to claim 1, its raw material is Tongkat Ali (Eurycoma
Longifolia), preferred Tongkat Ali tree root.
3. the preparation method of Tongkat Ali combination of active principles according to claim 1, its ultrasonic extraction with aqueous solution is characterized as eastern leather
Ali mixes with the aqueous solution after crushing according to mass volume ratio for 1: 1~30, and mixed liquor passes through continuous flow upstream ultrasonic extraction,
Time is 5min~5h, and ultrasonic extraction controls temperature to be 10~70 DEG C.
4. the preparation method of Tongkat Ali combination of active principles according to claim 1, its enrichment characteristics is aqueous extract with big
Hole resin adsorption enrichment, resinous type preferred HP-20, D101, DM130, AB-8.
5. the preparation method of Tongkat Ali combination of active principles according to claim 1, its resin column adsorption component elution characteristic exists
One or more of solvent mixing gradient elutions in ethanol, ethyl acetate, water are used in resin column.
6. the preparation method of Tongkat Ali combination of active principles according to claim 1, its filter membrane classification separation selection microfiltration membranes,
The one or more of gradient processing of milipore filter, reverse osmosis membrane and sodium filter membrane.
7. the preparation method of Tongkat Ali combination of active principles according to claim 1, its Tongkat Ali group of effective components A is main
For quassinoids material, preferably eurycomanone, content is 1~35%.
8. the preparation method of Tongkat Ali combination of active principles according to claim 1, its Tongkat Ali group of effective components B is main
For active polysaccharide, glycopeptide and amino acid.
9. the preparation method of Tongkat Ali combination of active principles according to claim 1, it is characterised in that obtained according to this method
Tongkat Ali combination of active principles has enhancing sexual function, improves fertility-rate, antifatigue, strengthen immunity of refreshing oneself, increase body
, pain wind action can be alleviated.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610250227.0A CN107303303B (en) | 2016-04-22 | 2016-04-22 | Prepare Tongkat Ali active ingredient and combination application |
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| CN201610250227.0A CN107303303B (en) | 2016-04-22 | 2016-04-22 | Prepare Tongkat Ali active ingredient and combination application |
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| CN107303303A true CN107303303A (en) | 2017-10-31 |
| CN107303303B CN107303303B (en) | 2018-05-11 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400746A (en) * | 2018-11-23 | 2019-03-01 | 华南理工大学 | One kind having immunological regulation and anti-erythrocyte hemolytic activity Tongkat Ali polysaccharide and preparation method thereof |
| CN109456418A (en) * | 2019-01-17 | 2019-03-12 | 齐齐哈尔大学 | A kind of activity Tongkat Ali polysaccharide and preparation method |
| CN111303180A (en) * | 2020-02-21 | 2020-06-19 | 湖南中茂生物科技有限公司 | Method for extracting eurycomanone from eurycoma longifolia |
| CN112843050A (en) * | 2021-02-02 | 2021-05-28 | 天津中医药大学 | Application of quassin diterpenoid in preparation of medicine for treating cholestatic liver disease |
| CN113425765A (en) * | 2021-07-14 | 2021-09-24 | 重庆森康道生命健康研究院有限公司 | Uric acid reducing medicine and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400746A (en) * | 2018-11-23 | 2019-03-01 | 华南理工大学 | One kind having immunological regulation and anti-erythrocyte hemolytic activity Tongkat Ali polysaccharide and preparation method thereof |
| CN109400746B (en) * | 2018-11-23 | 2021-02-12 | 华南理工大学 | Eurycoma longifolia polysaccharide with immunoregulation and anti-erythrocyte hemolysis activity and preparation method thereof |
| CN109456418A (en) * | 2019-01-17 | 2019-03-12 | 齐齐哈尔大学 | A kind of activity Tongkat Ali polysaccharide and preparation method |
| CN109456418B (en) * | 2019-01-17 | 2020-10-27 | 齐齐哈尔大学 | Active eurycoma longifolia polysaccharide and preparation method thereof |
| CN111303180A (en) * | 2020-02-21 | 2020-06-19 | 湖南中茂生物科技有限公司 | Method for extracting eurycomanone from eurycoma longifolia |
| CN112843050A (en) * | 2021-02-02 | 2021-05-28 | 天津中医药大学 | Application of quassin diterpenoid in preparation of medicine for treating cholestatic liver disease |
| CN113425765A (en) * | 2021-07-14 | 2021-09-24 | 重庆森康道生命健康研究院有限公司 | Uric acid reducing medicine and preparation method thereof |
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| CN107303303B (en) | 2018-05-11 |
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Effective date of registration: 20230802 Address after: No. 3 Huitong Road, Zhonghe Industry City Integration New Area, Bianhe District, Tengchong City, Baoshan City, Yunnan Province, 679118 Patentee after: Yunnan lorry Biotechnology Co.,Ltd. Address before: 102206 Beijing science and Technology Park Changping District North Road 7 technology complex building C0302 Patentee before: QINGLUO (BEIJING) CRUDE DRUG Co.,Ltd. |