CN107595893A - A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof - Google Patents
A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof Download PDFInfo
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Abstract
A kind of preparation method of the enzyme treated prepared slices of Chinese crude drugs, single Chinese medicine is extracted using enzyme according to different classes of Chinese medicine first, then filters the dregs of a decoction, the dregs of a decoction are again through water/alcohol second extraction, divide and take filtrate, concentrate, be processed into the various forms of prepared slices of Chinese crude drugs or water-insoluble（It is scattered）Granule.Described enzyme cellulase, zytase, amylase, more than a kind of carbohydrase；（2）Animal tcm material using protease, pancreatin, lipase, more than a kind of beta glucan enzyme；（3）Starch Chinese medicine using amylase, glucose oxidase, catalase, more than a kind of beta glucan enzyme；（4）Fruit class Chinese medicine using cellulase, pectase, carbohydrase, more than a kind of beta glucan enzyme；The recovery rate of the traditional Chinese medicine ingredients of enzyme extraction of the present invention improves 20 more than 30% than common water extraction, alcohol water extraction, the advantages of being also fully used with pollution-free, limited resources.
Description
Technical field
The present invention relates to traditional Chinese medicine extraction technology and application field, specially a kind of single medicinal material or Chinese prescription are enzyme treated
Manufactured prepared slices of Chinese crude drugs and preparation method thereof.
Background technology
Chinese medicine is the rarity of the Chinese nation, and before thousands of years, Chinese ancients have understood to be cured the disease using Chinese medicine, from The Yellow Emperor's Canon of Internal Medicine
To Compendium of Materia Medica, sparkle with the radiance and wisdom of the Chinese nation invariably so that the Chinese nation can health multiply and live and have
The culture and history of brilliance, since the founding of People's Republic of China, particularly since China's reform and opening-up, China is in utilization
The various diseases of herbtherapy achieve great achievement highly visible, many insurmountable illnesss of doctor trained in Western medicine, by traditional Chinese medicine
Treatment, achieves good effect.
But Chinese medicine is cured the disease there is also some shortcomings, for example, prescription medicine volume it is big, it is necessary to plus be decocted in water for oral dose, even if boiling rear medicine
Composition does not make full use of also, and the dregs of a decoction discard very unfortunately, so Chinese medicine processing at present uses water extraction mostly, organic solvent carries
Follow the example of, water logging ultrasonic extraction, also someone has used enzyme extraction method, but does not cause that traditional Chinese medicine extraction is complete completely also, medicine
Slag also contains many useful components.
We introduce conventional several Traditional Chinese medicine extraction methods below：
（One）Solvent extraction method：
1. the principle of solvent extraction method：Solvent extraction method is the dissolution properties according to various composition in Chinese herbal medicine in a solvent, choosing
With big to active ingredient solubility, to not needing the small solvent of dissolved element solubility, and by active ingredient out of medicinal material tissue
The method being dissolved out.When solvent is added to herbal raw material（Need to suitably it crush）When middle, solvent is gradual due to diffusion, osmosis
Penetrated into the cell by cell membrane, dissolved solable matter, and cause the concentration difference of intraor extracellular, it is then intracellular dense
Constantly to external diffusion, solvent constantly enters in medicinal material histocyte, so repeatedly come and gone solution again, until intraor extracellular solution is dense
When degree reaches dynamic equilibrium, this saturated solution is filtered out, continues repeatedly to add novel solvent, it is possible to be bordering on required composition
Complete dissolution or big portion's dissolution.
Common Extraction solvent can be divided into following three class：
（1）Water：Water is a kind of strong polar solvent.Hydrophilic composition in Chinese herbal medicine, as inorganic salts, carbohydrate, molecule are less big
Polysaccharide, tannin, amino acid, protein, acylate, alkaloid salt and glucoside etc. can by it is water-soluble go out.In order to increase certain
The solubility of a little compositions, also frequently with sour water and buck as Extraction solvent.Sour water extracts, and can make alkaloid and acid generation salt
And dissolution, buck extraction can make organic acid, flavones, anthraquinone, lactone, cumarin and phenols component dissolution.But extracted with water easy
Glycosides composition is digested, and easily mould evil idea goes bad.Some Chinese herbal medicines containing pectin, mucilaginous substance constituents, its aqueous extract are often difficult to
Filtering.During extracting in boiling water, the starch in Chinese herbal medicine can be gelatinized, and increase the difficulty of filtering.Therefore the Chinese herbal medicine more than starch-containing amount,
Added water to cook after fine powder should not be worn into.The decoction of Chinese medicine tradition, use the straight fire of the prepared slices of Chinese crude drugs and decoct, heating can increase Chinese medicine
Outside the solubility of composition, it is also possible to have and produce " hydrotropy " phenomenon with other compositions, add that solubility in some water is small, lipophilic
The solubility of the strong composition of property.But solubility of most lipophilic compositions in boiling water is little, both make there is hydrotropy phenomenon to deposit
Also it is complete to be not easy extraction.If boiled using a large amount of decoctings, difficulty during evaporation and concentration will be increased, and meeting dissolution is a large amount of
Impurity, made troubles to further separating-purifying.Contain saponin and mucilaginous substance constituents in Chinese herbal medicine aqueous extract, it is dense depressurizing
During contracting, a large amount of foams can be also produced, cause the difficulty of concentration.Anti-splash ball generally can be separated by one liquid of vapour one of device on distiller
Overcome, industrially then typical thin films enrichment facility.
（2）Hydrophilic organic solvent：It is namely general described with the miscible organic solvent of water, such as ethanol（Wine
Essence）, methanol（Another name for）, acetone etc., it is the most frequently used with ethanol.The solubility property of ethanol is relatively good, and energy is penetrated to Chinese herbal medicine cell
Power is stronger.Hydrophilic composition isolating protein, mucilaginous substance, pectin, starch and par-tial polysaccharide etc. are outer, and big multipotency is molten in ethanol
Solution.The lipophilic composition of water is insoluble in, solubility in ethanol is also larger.It can also be adopted according to the property for being extracted material
Extracted with the ethanol of various concentrations.More less than water consumption with ethanol extraction, extraction time is short, dissolves the water-solubility impurity
Also it is few.Ethanol is organic solvent, though inflammable, small toxicity, cheap, convenient sources, has a locking equipment to reclaim repeatedly
Use, and the extract solution of ethanol is not easy moldy metamorphism.For these reasons, the method extracted with ethanol is always the most frequently used
One of method.The property of methanol is similar with ethanol, and boiling point is relatively low（64℃）, it is but toxic, it should be noted that during use.
（3）Lipophilic organic solvent：It is namely general described with the not miscible organic solvent of water, as petroleum ether,
Benzene, chloroform, ether, ethyl acetate, dichloroethanes etc..The selection performance of these solvents is strong, it is impossible to or be not easy to propose hydrophily
Impurity.But this kind of solvent volatility is big, how inflammable（Except chloroform）, general poisonous, expensive, equipment requirement is higher, and it
Penetrate plant tissue ability it is weaker, generally require for a long time extraction repeatedly could extract it is complete.If in medicinal material contain compared with
More moisture, it is difficult to leach its active ingredient with this kind of solvent, therefore, when largely extracting herbal raw material, directly using this
Class solvent has certain limitation.
2. extracting method：With solvent extraction medicinal herb componentses, commonly use infusion process, percolation, decocting method, reflux extraction and
Continuous circumfluence extraction method etc..Meanwhile the factor such as the degree of grinding of raw material, extraction time, Extracting temperature, appointed condition can also influence
Extraction efficiency, it is necessary to take in.
（1）Infusion process：Impregnate the genealogy of law to fill herbal powder or fragment in the appropriate container of people, add suitable solvent
（Such as ethanol, dilute alcohol or water）, dipping medicinal material is in the method for dissolution wherein composition.This law is relatively simple, but leaching rate is poor,
And be such as solvent with water, its extract solution is easy to moldy metamorphism）Notably add appropriate preservative.
（2）Percolation：Percolation is that herbal powder is mounted in percolator, constantly adds novel solvent, penetrates it
Medicinal material is small when solvent infilters medicinal powder dissolved element proportion from a kind of leaching method of percolator bottom outflow leachate from top to bottom
When increasing and moving down, its position is just replaced in the solution on upper strata or dilute immersion liquid, causes good concentration difference, makes diffusion energy preferable
Ground is carried out, therefore leaching effect is better than infusion process.But coutroi velocity is answered, supplements novel solvent from powder at any time in transient is oozed,
Make untill active ingredient fully leaches in medicinal material.When ooze dropping liquid color it is extremely shallow or ooze gush the volume of liquid equivalent to：Crude drug weight
10 times when, can think substantially to have extracted completely.Often using collection dilute, to ooze Huaihe River liquid new as another batch in a large amount of productions
The solvent of raw material is used.
（3）Enzyme extracts：The principle of enzyme extraction is the high specificity using enzyme reaction, and the constituent of cell membrane is hydrolyzed
Or degraded, cell membrane is destroyed, so as to improve the recovery rate of active ingredient.Enzymatic Extraction requires that enzyme has high activity, height
Selectivity and mild reaction conditions.Enzyme can be used as extraction adjuvant in the extraction of plant medicinal component, in animal drugs extraction
Activator and hair remover etc. can be used as, the catalyst that can be recycled as the fining agent and the dregs of a decoction of extract solution.
The effect of Enzymatic Extraction depend primarily on the species of enzyme, dosage, enzymolysis time, temperature, acid-base value, fineness of materials,
The many factors such as stirring.It is gentle, selective strong to be enzymatically treated use condition.On the one hand by degrading plant cell membrane make effectively into
Divide and be more easy to extraction so as to reach the purpose for improving extract yield or lowering solvent-oil ratio；On the other hand can be directed in autonomic drug
Most of impurity（Starch, pectin, protein etc.）Degradation selectivity, so that extraction separation is more easy to carry out, and meanwhile it is also comprehensive
Using the dregs of a decoction, turn waste into wealth.
At home, the factory of Shanghai Chinese medicine one is successfully prepared for SHENGMAI YIN KOUFUYE using enzyme process first.It is presently used for Chinese medicine
It is cellulase to study in terms of extraction more, and the cell membrane of most Chinese medicine is made up of cellulose, plant it is effective
Composition is often wrapped in cell membrane；Cellulose is then to use cellulase by β-D-Glucose with Isosorbide-5-Nitrae-β glucoside key connections
Enzymolysis can destroy β-D-Glucose key, destroy plant cell wall, be advantageous to the extraction to active ingredient.
Existing research report is exemplified below：Lv Weiming etc. describes a kind of new side of the extraction separation baicalein from radix scutellariae
Method-enzyme hydrolysis method, and compared with direct extraction method, as a result baicalein is up to 75.67%, yield in crude product obtained by new method
2.46%. Ma Tiantian is pre-processed through cellulase before extracting jamaicin with golden cypress, can improve jamaicin yield, and with not
Enzyme-added extraction is compared, and has significant difference.
Application of cellulase is extracted diosgenin by Zhang Caixia etc. in rhizoma dioscoreae nipponicae, its technique only step more than former technique
To the enzymolysis processing of crude drug medicine materical crude slice, but in the presence of cellulase, the yield of diosgenin, two methods pair are improved
Than there is significant difference.
Ma Juyun etc. is digested, with former extraction process phase before Herba Andrographitis extracts andrographolide through cellulase
Compare, improve the content and extracted amount of andrographolide.
Ma Juyun, Zhao Jingyan etc. have studied its enzyme-added group and not enzyme-added group to active ingredient from coptis extraction jamaicin
The influence of jamaicin extraction, new technology add the enzymolysis process of cellulase, but two than the former technique step that has been more thereto
There were significant differences for the content of berberine of kind technique extraction, and the composition extracted is consistent.
Someone extracts general flavone in the presence of fiber rope enzyme from the root of kudzu vine, and yield improves 13%.Extracted in pseudo-ginseng
Cheng Zhong, with cellulase degradation action breaks down cell membrane, the total soap former times recovery rate of pseudo-ginseng improves 23.5%.
In addition, industrial fiber element enzyme is applied in Chinese medicine and the dregs of a decoction by Hou Rongqiao etc. first, make the fiber of Chinese medicine and the dregs of a decoction
Element enzymolysis is β-glucose, and change slag is turned waste into wealth into medicine, and these analytes can be used as feed or fertilizer etc., reduce pollution.
Recently, the method for extracting Chinese medicine with enzyme also has some patent literatures, such as：
1. Chinese patent, woman's inflammation recovering particles and its production method, apply for (patent right) people：University Of Jishou, application number：
CN201410144919.8, open (bulletin) number：CN103877520A, summary：The invention discloses a kind of woman's inflammation recovering particles and
Its production method, comprising（1）After 11 kinds of Chinese medicine dispensings needed for 'Fuyankang ' tablet production in pharmacopeia, with complex enzyme formulation enzyme
Solution, then ultrasonic assistant refluxing extraction；（2）Chinese yam and curcuma zedoary（Vinegar is processed）The preparation of powder：With super after Chinese yam and curcuma zedoary are dried
Velocity of sound airflow comminution system and cold slurry crushing system crush；（3）The preparation of softwood：By Chinese yam powder and curcuma zedoary（Vinegar is processed）Powder with
Softwood is made in woman's inflammation health medicinal extract；（4）The preparation of particle：By soft material granulation and drying.The present invention using complex enzyme formulation enzymolysis and
It ultrasonic assistant refluxing extraction, can effectively shorten extraction time, improve the recovery rate of effective component of chinese medicine particularly matrine, carry
High product quality and qualification rate；Chinese yam and curcuma zedoary are crushed using supersonic jet mill system and cold slurry crushing system,
Good crushing effect, and sterilization steps can be saved.
2nd, Chinese patent：The extracting method of danshinolic acid constituents, application number in a kind of Salvia miltiorrhiza stem：
CN201610016850.X, publication number：CN105535100A, apply for (patent right) people：Taishan Hospital, summary：The present invention
A kind of extracting method of danshinolic acid constituents in Salvia miltiorrhiza stem is disclosed, it is characterized in that：Including multiplex-enzyme extraction and flooding, institute
Complex enzyme is stated as crude enzyme liquid caused by the crisp handle mushrooms of Bai Huang little (Psathyrella candolleana) BDF15 fermentations, it is described white yellow
Small crisp handle mushroom (Psathyrella candolleana) BDF15 has been preserved in Chinese microorganism strain guarantor on November 4th, 2015
Hiding administration committee's common micro-organisms center, (abbreviation CGMCC, address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), protect
Tibetan number is CGMCC No.11415.The extracting method of the present invention can be effectively facilitated the release of liposoluble ingredient in Salvia miltiorrhiza stem,
Recovery rate is improved, reduces production cost；And harmful substance is not produced in production process of the present invention, pollution-free, good product quality,
Effect is strong, while also comprehensively utilizes traditional Chinese medicine waste, turns waste into wealth, and is a kind of environment-friendly green extraction method.
3rd, Chinese patent, the combined-enzyme method extraction process of mother chrysanthemum flower flavone compound, application number：
The CN201310604362.7 applyings date：Open 2013.11.22 (bulletin) number：CN104644715A discloses (bulletin) day：
2015.05.27 apply for (patent right) people：Fang Shiping, summary：The present invention provides a kind of the compound of mother chrysanthemum flower flavone compound
Extraction Processes, belong to technical field of extraction of Chinese traditional medicine.Its extraction step includes：Chrysanthemum indicum is dried, crushed, it is left to cross 300 mesh
Right sieve；Weigh dry powder and add extractor, it is solvent to add absolute ethyl alcohol by liquid ratio；Complex enzyme is added into tank, controls tank
The conditions such as interior pH value, temperature, digest, filtering；Filter vacuum is dried under reduced pressure, -20 DEG C be kept in dark place it is standby.The present invention is most normal
On the basis of traditional handicraft ethanol extraction method is slightly modified, newest bioengineering enzyme engineering multiplex-enzyme extraction is combined
Method, it is simple applied to actual production, low raw-material cost, operation, time-saving and efficiency, significantly improve recovery rate and purity.
4th, Chinese patent, a kind of preparation method of swelling and pain relieving Chinese medicine composition, application number：CN201611157572.6,
Open (bulletin) number：CN106492129A, open (bulletin) day：2017.03.15 apply for (patent right) people：Zhuang nationality in Guangxi
Premium medicine company limited company of autonomous region makes a summary：The invention discloses a kind of preparation method of swelling and pain relieving Chinese medicine composition,
Belong to Chinese patent medicine preparation preparation method field, weigh the banksia rose, windproof, schizonepeta, asarum, cortex acanthopanacis, cassia twig, the root of bidentate achyranthes, Ligusticum wallichii, Xu's length
Minister in ancient times, the root of Dahurain angelica, curcuma zedoary, red bark of eucommia, great Luo umbrellas, punctate ardisia herb, Radix zanthoxyli, herba fibraureae recisae, cape jasmine, Rhizoma Sparganii, agalloch eaglewood, camphor, menthol 20
Medicinal material simply, micron ball mill grinding, negative pressure of vacuum maceration enzyme extracts after drying, and micro-filtration, produces.The enzyme extraction skill of the present invention
Art, carry gained active ingredient purity it is higher, inorganic ceramic UF membrane can be active constituent-enriched, and extraction efficiency is higher, negative pressure of vacuum
Dipping can make the enzyme solutions of low concentration be sufficiently impregnated medicinal material fiber pore interior, reach the complete effect of extraction.
5. Chinese patent, the method for arasaponin, application number in a kind of fresh pseudo-ginseng of multiplex-enzyme extraction：
CN201410141879.1, open (bulletin) number：CN103933092A, open (bulletin) day：2014.07.23 application is (special
Economic rights) people：Wenshan Sanqi Research Institute of mountain of papers institute, summary：The present invention relates to the total soap of pseudo-ginseng in a kind of multiplex-enzyme extraction Chinese medicine
The method of glycosides, belong to the field of Chinese medicines.The method of arasaponin in more particularly to a kind of fresh pseudo-ginseng of multiplex-enzyme extraction, the present invention use
Fresh pseudo-ginseng is pelletized to extract raw material by crushing, enzymolysis processing, merging filtrate concentration, and alcohol precipitation processing, elution is handled and taken off
Beta amylase is introduced into the new technology of saponin constituent in the fresh pseudo-ginseng of enzyme extraction by color processing so as to carry out the extraction of arasaponin
In, using fresh pseudo-ginseng as raw material, shorten the traditional mode of production cycle, improve the quality and recovery rate of arasaponin, the present invention is advantageous to
Market regulation, the control ability of Yunnan notoginseng, realize that the skill upgrading to enterprise of one's native land, economic benefit increase, so as to promote three
The sustainable development of seven industries.
It can be seen that ferment treatment Chinese medicine is a kind of developing direction of prepared slices of Chinese crude drugs processing.
The content of the invention
It is an object of the invention to provide a kind of ferment treatment Chinese medicine and preparing new-type medical herbs drink tablets and preparation method thereof are made.
Chinese medicine is enzyme treated to shorten extraction process, improves the utilization rate of traditional Chinese medicine ingredients, can be processed into pulvis, particle
Agent, pill（Containing micropill）, capsule or tablet form preparing new-type medical herbs drink tablets.The recovery rate ratio of the traditional Chinese medicine ingredients of enzyme extraction of the present invention
Common water extraction, alcohol-water extraction improve more than 20-30%；Moreover, biological enzyme is high except extraction efficiency, it has been also equipped with without dirt
Dye, do not easily cause limited resources waste beneficial effect.
Preparing new-type medical herbs drink tablets of ferment treatment and preparation method thereof of the present invention, it is in single using single enzyme or mixed enzyme
Medicinal material carries out enzyme extraction, then filters the dregs of a decoction, and the dregs of a decoction can divide again through water/alcohol second extraction and take filtrate, concentrate, be processed into Chinese medicine
Pulvis, granule, pill（Containing micropill）, capsule or tablet form preparing new-type medical herbs drink tablets.The preparation side of the described prepared slices of Chinese crude drugs
Method and common Chinese pulvis, granule, pill, the preparation method containing micropill, capsule or tablet is identical, or preparation method and auxiliary
Material is different.
Enzyme of the present invention is a kind of enzyme or a variety of enzymes in following each group：
（1）The plant roots and stems class Chinese medicine of fibre-enrich：Using cellulase, zytase, amylase, a kind of carbohydrase or 2 kinds
Enzyme above；
（2）Animal tcm material：Using protease, pancreatin, lipase, 1,4 beta-glucanase one kind or two or more enzyme；
（3）The abundant Chinese medicine of starch：Using amylase, glucose oxidase, catalase, a kind or 2 of 1,4 beta-glucanase
The enzyme of the kind above；
（4）Fruit class Chinese medicine：Using cellulase, pectase, carbohydrase, 1,4 beta-glucanase one kind or two or more enzyme.
The part by weight of Chinese medicine and enzyme of the present invention is 100:（0.1-5）.
The process conditions of enzymolysis of the present invention are：
（1）Chinese medicine is smashed respectively to the powder below 100 mesh；
（2）Add 20-80 DEG C of water immersion 3-5 hour；
（3）Every kind of Chinese medicine is separately added into enzyme, is digested, and filters the dregs of a decoction, obtains digesting decoction, concentration is standby；
（4）Second extraction, the dregs of a decoction add 3-10 times of boiling boiling 0.5-4 hour, are separated by filtration, waste, obtain water extraction decoction by weight
Concentrate standby；
（5）Enzymolysis concentrated medicament and water extraction concentrated medicament are done using microwave drying, spray drying process or cold drying
Dry, merging obtains powder；
（6）Powder is processed into pulvis, granule, pill, capsule or tablet by existing preparations shaping method；Or add bonding
Agent, the controllable slow release formulation of dissolution velocity is made.
Described adhesive is starch, natural gum or synthetic resin；Described synthetic resin includes acrylic resin, poly- ammonia
Ester, ethyl cellulose, zein and polyvinyl alcohol.
Described above（3）For the enzymolysis of step according to different materials, the time is 0.5-20 hours, and temperature is 20-80 DEG C.
Described（4）The second extraction of step can use solvent extraction, and described solvent is from ethanol, ether, acetic acid second
Ester or nontoxic volatilizable solvent.
Plant roots and stems class Chinese medicine, starch Chinese medicine and the fruit class Chinese medicine of above-described fibre-enrich are dry product
Or fresh goods.
When animal tcm material is the fresh animal meat or bone just slaughtered, it should cooked or dries pulverizing.
The extraction of mushroom Chinese medicine is by the processing of the plant roots and stems class Chinese medicine of starch Chinese medicine and fibre-enrich.
Filter separation method can with Ceramics UF membrane, the separating technology such as be centrifuged at a high speed, by the dregs of a decoction with it is soluble
Composition is separated.
Decoction can add proper amount of nano silica, talcum powder, antiplastering aid or other inserts before drying.
The middle drug metabolizing enzyme of the extraction, commercialization enzyme, or self-control enzyme can be selected.
Described（2）With（4）The water of step is pure water or mineral water.
The beneficial effects of the invention are as follows：
1st, have no that document introduction carries out enzyme classification extraction respectively to different types of Chinese medicine in Chinese medicine compound prescription at present, document is only
The same enzyme or multiplex-enzyme extraction whole prescription are used only for certain medicinal material or traditional Chinese compound medicine, may so cause to carry
Take not exclusively, active ingredient can not be all of.
2nd, the current prepared slices of Chinese crude drugs, it is exactly section or beats powder, the present invention is that these original prepared slices of Chinese crude drugs are carried out deep processing, is adopted
With ferment treatment, very big pole easily effective component extracting, the new of the forms such as pulvis, particle, pill, micropill, capsule, piece is made
Medicine materical crude slice, greatly facilitates practical, is greatly enhanced the stability of the prepared slices of Chinese crude drugs, solve the present prepared slices of Chinese crude drugs easily go mouldy, snake
The problems such as and inconvenient problem with use.Somewhat similar granule, granule, the agriculture we for training power, they are single
Taste Chinese medicine extracts by water extraction or ethanol, and particle is made, and the present invention is that single medicinal material material is extracted by enzyme, is made not
Only a form of medicine materical crude slice of particle, this new medicine materical crude slice, when use, the parcel of 1 parcel 1 is used, combined, just formed
One compound.
3, preparation method of the present invention is simple, and products obtained therefrom is convenient to take, evident in efficacy, can greatly speed up patient's postoperative rehabilitation
Speed.
4th, the recovery rate of enzyme extraction traditional Chinese medicine ingredients of the present invention improves more than 20-30% than common alcohol-water extraction；Moreover,
Biological enzyme is high except extraction efficiency, has been also equipped with the beneficial effect of waste that is pollution-free, not easily causing limited resources.
Embodiment
Embodiment 1
The Sijunzi Tang traditional Chinese medicine extraction of embodiment 1, the raw material composition of the following parts by weight of described Sijunzi Tang：Radix Codonopsis 30-50；In vain
Art 30-50；Radix glycyrrhizae 5-10；Poria cocos 30-50.
Crushed using by Radix Codonopsis, the bighead atractylodes rhizome, radix glycyrrhizae, Poria cocos, then extracted with enzyme respectively,
Radix Codonopsis uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, two kinds or two of carbohydrase
The enzyme extraction of the kind above；
The bighead atractylodes rhizome uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, two kinds or two of carbohydrase
The enzyme extraction of the kind above；
Radix glycyrrhizae uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, two kinds or two of carbohydrase
The enzyme extraction of the kind above；
Poria cocos is extracted using starch Chinese medicine enzyme：Using amylase, glucose oxidase, catalase, 1,4 beta-glucanase
Two or more enzyme.
Concrete technology is that Radix Codonopsis, the bighead atractylodes rhizome, radix glycyrrhizae, Poria cocos are added to 2-3 times of water by weight respectively, adds mixed enzyme, enters
Row enzymolysis, time are 4-10 hours, and temperature is 35-50 DEG C；Then the dregs of a decoction are filtered, using the decoction that is centrifuged at a high speed, concentration, are added
Granule or capsule is made into the auxiliary material such as Chinese medicine powder or the appropriate talcum powder starch of addition in work；
Sijunzi Tang is only with being decocted in water for oral dose at present.
Radix Codonopsis of the present invention, the bighead atractylodes rhizome belong to plant roots and stems fiber-like Chinese medicine, with cellulase, zytase, amylase, saccharification
Two or more enzyme of enzyme；
Radix glycyrrhizae (Glycyrrhiza uralensis Fisch),【Alias】State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet tea root.For beans
Section's liquorice root（Glycyrrhiza uralensis Fisch.）, swollen fruit Radix（Glycyrrhiza inflata Bat.）Or
Glycyrrhiza glabra（Glycyrrhiza glabra L.）Dry root.【Nature and flavor and channel tropism】It is sweet, put down.The thoughts of returning home, lung, spleen, stomach.
【Major function】Invigorate the spleen and benefit qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, coordinating the drug actions of a prescription.For weakness of the spleen and the stomach, lassitude hypodynamia,
Palpitation, coughing with a lot of sputum, gastral cavity abdomen, four limbs contraction, carbuncle sore tumefacting virus, alleviate drug toxicity, strong.Radix glycyrrhizae belongs to plant roots
Fasciated fiber class Chinese medicine, with cellulase, zytase, amylase, carbohydrase two or more enzyme.
Poria cocos（Wolfiporia cocos）,【Alias】Yun Ling, Song Ling, Fu spirit.For On Polyporaceae Poria cocos Poria
cocos （Schw.）Wolf dry sclerotia.【Nature and flavor and channel tropism】It is sweet, light, put down.The thoughts of returning home, lung, spleen, kidney channel.【Major function】
Clearing damp and promoting diuresis, invigorating the spleen calming heart.For oedema oliguria, phlegm retention anti-dazzle nervous, spleen eating less, loose stool diarrhea, confused and worried, insomnia of palpitating with fear.
Poria cocos uses starch Chinese medicine：Using amylase, glucose oxidase, catalase, two kinds or two of 1,4 beta-glucanase
The enzyme extraction of the kind above.By quantitative determination, extraction Radix Codonopsis, the bighead atractylodes rhizome, radix glycyrrhizae, the active ingredient of Poria cocos can improve 20-30%.
Embodiment 2
Huanglian jiedu decoction, huanglian jiedu decoction dosage, coptis 9g, radix scutellariae 6g, golden cypress 6g, cape jasmine 9g.
Huanglian jiedu decoction, tcm prescription name.For heat-clearing formula, there is the effect of clearing heat and detoxicating.Cure mainly three burnt fire-toxicity syndromes.Big heat
Agitation, dryness of the mouth and throat, paraphasia egersis；Or pyreticosis is spitted blood, bleeding from five sense organs or subcutaneous tissue；Or heat very sends out spot, or the lower profit of body heat, or jaundice with damp-heat pathogen；It is or outer
Section's large carbuncle serious case of furuncle.Dark urine, red tongue with yellowish fur, rapid pulse are strong.Clinic is usually used in treating septicemia, septicopyemia, dysentery, lung
The category heat toxin such as inflammation, urinary infection, meningococal meningitis, encephalitis B is patient.
The taste of Qingre Jiedu Tang four, radix scutellariae phellodendron and gardenia is standby, and manic big heat is vomitted egersis, and hematemesis hair spot can be.
Composition, the coptis, radix scutellariae, golden cypress, cape jasmine.Current usage, upper four taste, cuts, and with six liters of water, boils and takes two liters, divides two
Clothes.Modern usage：Decocting is boiled.
Function, purging intense heat and detonicating.
Huanglian jiedu decoction, cure mainly, three burnt fire-toxicity syndromes.Big heat is irritated, dryness of the mouth and throat, paraphasia egersis；Or pyreticosis is spitted blood, nosebleed
Blood；Or heat very sends out spot, or the lower profit of body heat, or jaundice with damp-heat pathogen；Or surgery large carbuncle serious case of furuncle.Dark urine, red tongue with yellowish fur, rapid pulse have
Power.
Huanglian jiedu decoction Fang Yi, assertive evidence is more caused by fiery poison is full of three Jiao.Treatment is based on purging intense heat and detonicating.Fiery poison is flourishing,
On disturb gods, therefore see dysphoria with smothery sensation delirious speech；Blood-head is forgotten to go, therefore to spit blood；Blood overflows skin, therefore sees hair spot；Excess heat hinders Tianjin, therefore sees the dry pharynx of mouth
It is dry；The red few tongue of tongue, rapid pulse are effectively the disease of excessive noxious heat.The clear purge the sthenic heart-fire of the coptis in side, the simultaneous fire for rushing down middle Jiao, it is monarch drug in a prescription；Radix scutellariae
The fire of the part of the body cavity above the diaphragm housing the heart and lungs is rushed down, is ministerial drug；Golden cypress rushes down the fire of lower Jiao；Cape jasmine rushes down the fire of three Jiao, and heat conduction is descending, draws heat symptoms caused by an exopathgen and goes out from urine.Two
Person is adjutant.
Huanglian jiedu decoction compatible experiments, radix scutellariae, the coptis, golden cypress compatibility rush down clearly three burnt fiery poisons, and cape jasmine draws heresy and gone out from urine.
Huanglian jiedu decoction uses, and we are used for three burnt fire-toxicity syndromes.Clinical practice is irritated with big heat, and dryness of the mouth and throat, pyreticosis is told
Blood, bleeding from five sense organs or subcutaneous tissue；Or heat very sends out spot, red tongue with yellowish fur, rapid pulse is effectively dialectical main points.
Huanglian jiedu decoction addition or subtraction of changes, constipation person, increase Huang and rush down lower burnt real heat；Haematemesis, bleeding from five sense organs or subcutaneous tissue, hair spot, add radix scrophulariae, life
Ground, the root bark of tree peony are with clearing heat and cooling blood；Jaundice person, increase yellow, oriental wormwood clearing heat and expelling damp removing jaundice；Sore swollen toxin person, add dandelion, the capsule of weeping forsythia with clear
Thermal detoxification.
The present invention is crushed the coptis, radix scutellariae, golden cypress, cape jasmine respectively, is then extracted with enzyme,
The coptis uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, one kind of carbohydrase or two
The enzyme of the kind above；
Radix scutellariae uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, one kind of carbohydrase or two
The enzyme of the kind above；
Golden cypress uses plant roots and stems fiber-like Chinese medicine：Using cellulase, zytase, amylase, one kind of carbohydrase or two
The enzyme of the kind above；
Cape jasmine uses fruit class Chinese medicine：Using cellulase, pectase, carbohydrase, 1,4 beta-glucanase two or more
Enzyme；
Concrete technology is that the coptis, radix scutellariae, golden cypress, cape jasmine are added to 2-3 times of water by weight respectively, adds mixed enzyme, carries out enzyme
Solution, time are 4-10 hours, and temperature is 35-50 DEG C；Then the dregs of a decoction are filtered, once, the decoction of merging concentrates dregs of a decoction extracting in water,
Using the decoction that is centrifuged at a high speed, concentration, it is processed into the auxiliary material such as Chinese medicine powder or the appropriate talcum powder starch of addition and granule is made
Or capsule；Huanglian jiedu decoction is only with being decocted in water for oral dose at present.By quantitative determination, the extraction coptis, radix scutellariae, the active ingredient of golden cypress
20-30% can be improved.
Embodiment 3, ganoderma lucidum Treated with Radix Salviae Miltiorrhizae coronary heart disease proved recipe
Prescription：15 grams of ganoderma lucidum, 20 grams of the red sage root, 10 grams of Ligusticum wallichii, 20 grams of wilsonii；Traditional extraction method is that the five tastes are added into 3-5 times of water
Infusion 2-4 hours, filtering, the obtained dregs of a decoction add water 2 times of water infusion 1-2 hours, merge decoction, are condensed into oral liquid, or
Paste is condensed into, auxiliary material is added, makes capsule or tablet.
Above-mentioned proved recipe has enhancing myocardial contraction, improves blood circulation, reduces myocardial oxygen consumption, cardiac nutrition, improves the heart
Dirty function, hypoglycemic and other effects, cure mainly angina pectoris, arrhythmia cordis, miocardial infarction, coronary heart disease etc..
But above-mentioned water extraction can only use the 50-60% of effective ingredient, particularly ganoderma lucidum, its GL-B and flavones
Element.
The present invention uses enzymatic isolation method, is crushed respectively after ganoderma lucidum, the red sage root, Ligusticum wallichii, wilsonii are dried, then that ganoderma lucidum is fine
Tie up enzyme, carbohydrase, 1,4 beta-glucanase and catalase multiplex-enzyme extraction；
The red sage root and Ligusticum wallichii cellulase, zytase multiplex-enzyme extraction；
Wilsonii amylase, carbohydrase zytase multiplex-enzyme extraction；
Concrete technology is that ganoderma lucidum, the red sage root, Ligusticum wallichii, wilsonii are added to 3-4 times of water by weight respectively, adds mixed enzyme, is carried out
Enzymolysis, time are 4-10 hours, and temperature is 35-50 DEG C；Then the dregs of a decoction are filtered, the dregs of a decoction add alcohol reflux extraction once, reclaim second
Alcohol, the decoction concentration of merging, is processed into oral liquid, Chinese medicine powder, granule or capsule；
By quantitative determination, 20-30% can be improved by extracting the active ingredient of ganoderma lucidum.
Embodiment 4, train of thought Shutong granules are the line use when participating in the cintest currently used for peripheral artery diseases such as treatment vasculitis, phlebitis
Medicine.
Main Ingredients and Appearance：The Radix Astragali, honeysuckle, golden cypress, rhizoma atractylodis, coix seed, radix scrophulariae, Radix Angelicae Sinensis, the root of herbaceous peony, radix glycyrrhizae, leech, centipede,
Scorpio.All medicines share, clearing heat and detoxicating, disperse blood stasis and dredge collateral, the work(of clearing damp detumescence are played altogether.
The superficial thrombophlebitis that train of thought Shutong granules are used for caused by dampness and heat stasis train of thought, non-acute phase DVT shape
Into caused lower limb swelling, pain, the colour of skin it is dark red or with streak thing.Usage and dosage：Take with warm water, once
20g (1 bag), 3 times a day.
The pharmacological action of leech：Leech water decoction has stronger blood coagulation resisting function, when can significantly extend fibrinous cohesion
Between, hirudo extract, hirudin have obvious inhibitory action to platelet aggregation, suppress thrombosis in rat body, to diffusing
Property intravascular coagulation has good therapeutic action.Hirude decoction can improve hemorheology.Energy reducing blood lipid, regression Atherosclerosis
Change patch, increase cardiac muscle nutritional blood flow, resist the change of arrhythmia cordis or obvious T ripples, ST sections caused by pituitrin
Change.Promote cephalophyma to absorb, mitigate surrouding brain tissue inflammatory reaction and oedema, alleviate intracranial pressure rise, improve blood circulation,
Protection brain tissue exempts from destruction.Also there is obvious inhibiting effect to subcutaneous hematoma.Leech water decoction has obvious protection to make to renal ischaemia
With can reduce serum urea nitrogen, creatinine level, there is obvious reduction to act on to elevated Serum Level of Tumor Necrosis Factor.Hirudin
Also there is inhibitory action to tumour cell.In addition, leech water decoction still plays the role of terminal pregnancy.
Remaining medicine is shown in Chinese medicine pharmacopeia.
The present invention uses enzymatic isolation method, respectively by the Radix Astragali, golden cypress, radix glycyrrhizae, rhizoma atractylodis, coix seed, radix scrophulariae, Radix Angelicae Sinensis, the root of herbaceous peony, water
Leech, centipede, scorpio crush respectively after drying, then by honeysuckle, golden cypress, rhizoma atractylodis, radix scrophulariae, Radix Angelicae Sinensis, root of herbaceous peony cellulase, saccharification
Enzyme extracts；
Coix seed, honeysuckle amylase, glucose oxidase, one kind or two or more enzyme extraction；
Leech, centipede, scorpio（Scorpion）Extracted respectively with the one or more kinds of enzyme of protease, pancreatin, lipase；
Concrete technology is respectively by the Radix Astragali, golden cypress, radix glycyrrhizae, rhizoma atractylodis, coix seed, radix scrophulariae, Radix Angelicae Sinensis, the root of herbaceous peony, leech, centipede, scorpio
3-4 times of water individually plus is by weight added, enzyme is added, is digested, the time is 4-10 hours, and temperature is 35-50 DEG C；Then cross
Filter the dregs of a decoction, the dregs of a decoction add alcohol reflux extraction once, reclaim ethanol, merging decoction concentration, be processed into oral liquid Chinese medicine powder or
Add auxiliary material and granule, pill is made（Containing micropill）, capsule or tablet form preparing new-type medical herbs drink tablets.
Enzyme extraction is carried out to single Chinese medicine using single enzyme or mixed enzyme, then filters the dregs of a decoction, the dregs of a decoction can be again through water/alcohol
Second extraction, divide and take filtrate, concentrate, processing.By quantitative determination, the active ingredient of extraction can improve 20-30%.
Embodiment 5, Herba Lysimachiae granule
【Composition】：Desmodium, radix glycyrrhizae, sucrose, glucose,
【Effect】：Eliminating dampness and heat, treating stranguria, detumescence.For heat gonorrhea, Sha Lin, urine is puckery to have a pain, jaundice, carbuncle swells furunculosis, and poisonous snake stings
Wound；Liver and bile stone, lithangiuria.For heat gonorrhea, especially kind treatment stone gonorrhoea disease,
Using enzymatic isolation method, crushed respectively after desmodium, radix glycyrrhizae are dried, then by desmodium cellulase, zytase, starch
Enzyme multiplex-enzyme extraction, the part by weight of every kind of enzyme is 1:1:1；
Concrete technology is the water that desmodium, radix glycyrrhizae are added to 3-4 times of addition by weight respectively respectively, adds mixed enzyme, is digested,
Time is 4-10 hours, and temperature is 35-50 DEG C；Then the dregs of a decoction are filtered, the dregs of a decoction add alcohol reflux extraction once, reclaim ethanol, close
And decoction concentration, add acrylic resin or ethyl cellulose, sustained release pellet be made.
In summary, using ferment treatment, very big pole easily effective component extracting, pulvis, particle, pill, micropill, glue are made
The new medicine materical crude slice of the forms such as capsule, piece, practicality is greatly facilitated, the stability of the prepared slices of Chinese crude drugs is greatly enhanced, solves present Chinese medicine
The problems such as medicine materical crude slice easily goes mouldy, snake and inconvenient problem with use.Somewhat similar granule, granule, train power
Agriculture we, they are that single medicinal material material is extracted by water extraction or ethanol, particle are made, our this patent is single
Chinese medicine by enzyme extraction, more than a form of medicine materical crude slice of particle is made, this new medicine materical crude slice is small with regard to 1 parcel 1 when use
Bag uses, and combines, and just forms a compound.
The applicant is university of TCM, inventor is the professor of pharmacy of Chinese materia medica, through experiment in a few years, it was demonstrated that is adopted
With single enzyme or compound ferment treatment Chinese medicine, the effective component extraction rate of Chinese medicine can be significantly improved, particularly current resource is gradual
Using excess, many precious wild chinese herbal medicine resources have greatly reduced, and artificial growth does not catch up with also, so ancient using enzymatic treatment
Side, the new medicine materical crude slice of the forms such as pulvis, particle, pill, micropill, capsule, piece is made, greatly facilitate practicality, be greatly enhanced
The stability of the prepared slices of Chinese crude drugs, solve the present prepared slices of Chinese crude drugs easily go mouldy, snake the problems such as and inconvenient problem with use, improve
Utilization rate, it is a method very effectively with reality.
Claims (10)
- A kind of 1. preparation method of the enzyme treated prepared slices of Chinese crude drugs, it is characterised in that：Using single enzyme or mixed enzyme in single Medicinal material carries out enzyme classification extraction, then filters the dregs of a decoction, and the dregs of a decoction can divide again through water or alcohol second extraction and take filtrate, concentrate, be processed into The prepared slices of Chinese crude drugs；Described enzyme is a kind of enzyme or a variety of enzymes in following each group：（1）The plant roots and stems class Chinese medicine of fibre-enrich：Using cellulase, zytase, amylase, a kind of carbohydrase or 2 kinds Enzyme above；（2）Animal tcm material：Using protease, pancreatin, lipase, 1,4 beta-glucanase one kind or two or more enzyme；（3）The abundant Chinese medicine of starch：Using amylase, glucose oxidase, catalase, a kind or 2 of 1,4 beta-glucanase The enzyme of the kind above；（4）Fruit class Chinese medicine：Using cellulase, pectase, carbohydrase, 1,4 beta-glucanase one kind or two or more enzyme；The part by weight of the Chinese medicine and enzyme is 100:（0.1-5）The process conditions of the enzymolysis are：（1）Chinese medicine is smashed respectively to the powder below 100 mesh；（2）Add 20-80 DEG C of water immersion 3-5 hour；（3）Every kind of Chinese medicine is separately added into enzyme, is digested, and filters the dregs of a decoction, obtains digesting decoction, concentration is standby；（4）Second extraction, the dregs of a decoction add 3-10 times of boiling boiling 0.5-4 hour, are separated by filtration, waste, obtain water extraction decoction by weight Concentrate standby；（5）Enzymolysis concentrated medicament and water extraction concentrated medicament are done using microwave drying, spray drying process or cold drying Dry, merging obtains powder；（6）Powder is processed into pulvis, granule, pill, capsule or tablet by existing preparation or prescription；Or add bonding Agent, the controllable slow release formulation of dissolution velocity is made.
- 2. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that described（3）Step Enzymolysis according to different materials, the time is 0.5-20 hours, and temperature is 20-80 DEG C.
- 3. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that described（4）Step Second extraction can use solvent extraction, described solvent is from ethanol, ether, ethyl acetate or nontoxic volatilizable molten Agent.
- 4. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that the plant of fibre-enrich Thing rhizome traditional Chinese medicinal materials, starch Chinese medicine and fruit class Chinese medicine are dry product or fresh goods.
- 5. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that work as animal tcm When material is the fresh animal meat or bone just slaughtered, it should cooked or dries pulverizing.
- 6. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that mushroom Chinese medicine carries Take and handled by the plant roots and stems class Chinese medicine of starch Chinese medicine and fibre-enrich.
- 7. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that filter separation method Can with Ceramics UF membrane, the separating technology such as be centrifuged at a high speed, the dregs of a decoction and soluble component are separated.
- 8. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that decoction can before drying To add proper amount of nano silica, talcum powder, antiplastering aid or other inserts.
- 9. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 1, it is characterised in that the extraction Chinese medicine Enzyme, commercialization enzyme, or self-control enzyme can be selected.
- 10. the preparation method of the enzyme treated prepared slices of Chinese crude drugs according to claim 2, it is characterised in that described bonding Agent is starch, natural gum or synthetic resin；Described synthetic resin includes acrylic resin, polyurethane, ethyl cellulose, zein And polyvinyl alcohol.
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| CN109846923A (en) * | 2019-01-23 | 2019-06-07 | 云南广福药业有限公司 | A kind of extracting method of Caulis Kadsurae |
| CN109833443B (en) * | 2019-04-12 | 2021-07-27 | 中国农业科学院特产研究所 | Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof |
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| CN113045520A (en) * | 2021-03-25 | 2021-06-29 | 江西新世纪民星动物保健品有限公司 | Extraction process of andrographolide |
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