CN109833443B - Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof - Google Patents
Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof Download PDFInfo
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Abstract
A composite sunflower disc composition for reducing uric acid and relieving gout and a preparation method and application thereof belong to the technical field of functional foods. In order to solve the problems of large side effect, complex components and the like of the existing gout treatment medicines, the invention optimizes the preparation methods of the sunflower disc extract, the coix seed extract and the lophatherum gracile extract by methods of extraction, enzymolysis, molecular weight interception and the like, and the obtained extract is rich in active glycoconjugate with small molecular weight, flavone, alkaloid and other effective components. The invention also provides a composition consisting of 80-120 parts by weight of sunflower disc extract, 10-30 parts by weight of coix seed extract, 5-15 parts by weight of lophatherum gracile extract and 50-70 parts by weight of maltodextrin and a preparation method thereof, and the invention can be used for developing health care products and medicines for improving gout and/or hyperuricemia.
Description
Technical Field
The invention belongs to the technical field of functional foods, and particularly relates to a composite sunflower disc composition for reducing uric acid and relieving gout, and a preparation method and application thereof.
Background
Gout is crystal-related arthropathy caused by deposition of monosodium urate (MSU), is directly related to hyperuricemia caused by purine metabolic disorder and/or reduction of uric acid excretion, mainly comprises acute paroxysmal arthritis, tophus formation, tophaceous chronic arthritis, urate nephropathy and uric acid urinary tract stones, and can cause joint disability and renal insufficiency in severe cases. Gout is often accompanied by abdominal obesity, hyperlipidemia, hypertension, type 2 diabetes, cardiovascular diseases and other manifestations.
In the acute attack stage, gout and hyperuricemia can be treated or relieved by medicines, but if certain comprehensive prevention and treatment measures are not taken after the symptoms in the acute stage are controlled, the normal state is difficult to automatically recover. At present, chemical medicines are mostly adopted for treating acute gout or hyperuricemia, although the chemical medicines have certain effect, the side effect is large, the stimulation to the gastrointestinal tract is very serious, and the phenomena of diarrhea, nausea, acid regurgitation and the like often occur after a patient takes the medicine, so the life quality of the patient is seriously influenced. Some health-care medicines claim to have the effects of relieving gout and reducing uric acid level, but the components are too complex, even more than 10 formulas are formed, the quality standard is difficult to control during production, and the effective components are not clear enough. And many have not been proven to be effective in animal or human trials.
Disclosure of Invention
In order to solve the problems of large side effect, complex components and the like of the existing gout treatment medicines, the invention provides a sunflower disc composition which is prepared from the following raw materials in parts by weight: 80-120 parts of sunflower disc extract, 10-30 parts of coix seed extract, 5-15 parts of lophatherum gracile extract and 50-70 parts of maltodextrin.
Preferably, the composition consists of the following raw materials in parts by weight: 100 parts of sunflower disc extract, 20 parts of coix seed extract, 10 parts of lophatherum gracile extract and 70 parts of maltodextrin.
The invention also provides a preparation method of the sunflower disc composition, which comprises the following steps:
1) preparing the sunflower disc extract:
a. pulverizing Helianthus annuus L, extracting with ethanol, filtering, collecting ethanol extractive solution, extracting the residue with water, filtering, collecting water extractive solution, mixing the ethanol extractive solution and the water extractive solution, concentrating, and centrifuging to obtain supernatant;
b. performing enzymolysis on the supernatant by using a complex enzyme preparation, and sequentially filtering and drying enzymolysis liquid to obtain a sunflower disc extract;
2) preparation of coix seed extract: extracting Coicis semen with ethanol, removing ethanol, subjecting the extractive solution to enzymolysis with complex enzyme preparation, sequentially filtering and drying the enzymolysis solution to obtain Coicis semen extract;
3) preparing a lophatherum gracile extract: pulverizing folium Bambusae, extracting with ethanol, removing ethanol, filtering extractive solution, and drying to obtain folium Bambusae extract;
mixing the sunflower disc extract, the coix seed extract, the lophatherum gracile extract and maltodextrin in parts by weight to obtain the sunflower disc composition.
Further limited, the alcohol extraction in the step 1) refers to that the alcohol extraction is performed according to the weight ratio of 1 kg: adding 50% ethanol solution into the sunflower disc powder at a material-liquid ratio of 5L, and reflux-extracting at 55-62 deg.C for 1.5-2.5 h; the water extraction is to add water into the residue according to the proportion of adding 8L of water into 1kg of raw materials, and reflux-extract the residue for 1.5 to 2.5 hours at the temperature of between 55 and 62 ℃.
Further limiting, the enzymolysis in the step 1) refers to adding the complex enzyme preparation into the supernatant according to the proportion of adding 10g of the complex enzyme preparation into 1kg of raw materials, and reacting for 25-35 min at 45-50 ℃; the compound enzyme preparation consists of pectinase, glucoamylase, beta-glucanase and neutral xylanase, wherein the mass ratio of the enzymes is as follows: and (3) pectinase: glucoamylase: beta-glucanase: neutral xylanase = (8-12): (3-7): (0.8-1.2).
Further limited, the alcohol extraction in the step 2) is performed according to the ratio of 1 kg: (3-7) adding 20% ethanol solution into Coicis semen at a volume fraction of 20-L, and reflux-extracting at 55-62 deg.C for 1.5-2.5 h; the enzymolysis refers to adding the complex enzyme preparation into the extract according to the proportion of adding 0.5-1 g of the complex enzyme preparation into 1kg of the raw materials, and reacting for 25-35 min at 37 ℃; the compound enzyme preparation consists of pectinase and alpha-amylase, wherein the mass ratio of the enzymes is as follows: and (3) pectinase: α -amylase = 3: 1.
further limited, the alcohol extraction in step 3) is performed according to the weight ratio of 1 kg: (6-10) adding 80% ethanol solution into the crushed lophatherum gracile leaves according to the material-liquid ratio, and performing reflux extraction at 55-62 ℃ for 1.5-2.5 h.
Further limiting, the filtering in the step 1) is to filter the enzymolysis solution by gauze of 100 meshes and then filter the enzymolysis solution by a microporous filter membrane with the pore diameter of 4 mu m; the filtration in the step 2) refers to the nanofiltration filtration of the enzymolysis liquid; the filtration in the step 3) is a membrane filtration and classification method, and components with the molecular weight of 100-5000 Da are intercepted; the drying in steps 1) -3) is spray drying.
The invention also provides a sunflower disc solid beverage containing the sunflower disc composition.
The sunflower disc composition can be used for preparing medicines for relieving or treating gout and hyperuricemia.
Advantageous effects
The invention provides the sunflower disc composition which is relatively simple in composition, clear in functional components, proved by animal experiments in function and free of side effect.
In the preparation process of the sunflower disc composition, the extraction and concentration are performed at low temperature of about 60 ℃, so that the structure and texture properties of saccharides and polyphenols in an aqueous extract can be effectively prevented from being damaged by high temperature.
Mouse experiments show that the sunflower disc composition prepared by the invention can effectively relieve gout symptoms, remarkably reduce the blood uric acid level of hyperuricemia mice, and can be used for relieving gout and reducing the development of hyperuricemia drugs.
The invention establishes a preparation method of a sunflower disc extract, a coix seed extract and a lophatherum gracile extract, and provides a solid beverage which takes the 3 raw materials as main components and has the effect of treating gout and hyperuricemia, wherein the same products are not available in the market.
Detailed Description
The raw materials used in the present invention are commercially available, for example, pectinase, glucoamylase, beta-glucanase, neutral xylanase, pectinase and alpha-amylase, all available from Xiusheng enzyme Biotechnology, Inc., Cangzhou.
Example 1. preparation of sunflower disc composition.
The sunflower disc composition of the embodiment comprises the following raw materials in parts by weight: 100 parts of sunflower disc extract, 20 parts of coix seed extract, 10 parts of lophatherum gracile extract and 70 parts of maltodextrin. The preparation method comprises the following steps:
1) preparing the sunflower disc extract:
crushing a sunflower disc, adding ethanol for ethanol extraction, filtering after extraction, collecting ethanol extract, adding water into residues for water extraction, filtering and collecting water extract, combining the ethanol extract and the water extract, concentrating the obtained extraction mixed solution, and centrifuging to obtain supernatant;
b. and (3) carrying out enzymolysis on the supernatant by using a compound enzyme preparation, and sequentially filtering and drying the enzymolysis liquid to obtain the sunflower disc extract.
The specific method comprises the following steps:
a. pulverizing the sunflower disc, sieving with a 10-mesh sieve, and mixing according to the weight ratio of 1 kg: adding 50% ethanol solution into the sunflower disc powder at a material-liquid ratio of 5L, and extracting under reflux at 60 deg.C for 2 hr; filtering, and collecting alcohol extract; adding water into the residue according to the ratio of 1kg raw material to 8L water, extracting at 60 deg.C for 2 hr for 2 times, mixing the ethanol extract and water extract, concentrating the obtained extractive mixture at 60 deg.C to fixed ratio (1 kg raw material: 1L concentrated solution), centrifuging the concentrated solution (3000 rpm for 10 min), and removing the precipitate to obtain supernatant;
b. adding complex enzyme preparation (1L concentrated solution: 10g complex enzyme) into the supernatant, reacting at 47 deg.C for 30 min while stirring. After the reaction is finished, boiling water bath is carried out for 5min, and the enzyme is inactivated; the compound enzyme preparation consists of pectinase, glucoamylase (GAL type), beta-glucanase (BGL type) and neutral xylanase, wherein the weight ratio of the pectinase: glucoamylase (GAL type): beta-glucanase (BGL type): neutral xylanase = =10:5:1:1 (mass ratio), and the enzyme is made in domestic food grade. After enzymolysis, filtering the enzymolysis solution by using gauze of 100 meshes, and filtering by using a microporous filter membrane (4 mu m) to remove macromolecular substances; spray drying the filtrate to obtain sunflower disc extract.
2) Preparation of coix seed extract: extracting Coicis semen with ethanol, removing ethanol, subjecting the extractive solution to enzymolysis with complex enzyme preparation, filtering the enzymolysis solution, and drying to obtain Coicis semen extract. The specific method comprises the following steps:
drying coix seeds, and mixing the dried coix seeds according to the weight ratio of 1 kg: adding 20% ethanol solution into Coicis semen at a material-to-liquid ratio of 4L, reflux extracting at 60 deg.C for 2 hr, repeating extracting for 2 times, filtering, mixing filtrates, and rotary evaporating under reduced pressure to remove ethanol; adding pectinase: α -amylase = 3: 1 (mass ratio) of complex enzyme, reacting at 37 deg.C for 30 min, heating to inactivate enzyme, removing macromolecular substances above 50 kDa with nanofiltration equipment (aperture of 0.22 μm), and spray drying the filtrate to obtain Coicis semen extract.
3) Preparing a lophatherum gracile extract: pulverizing folium Bambusae, extracting with ethanol, removing ethanol, filtering the extractive solution, and drying to obtain folium Bambusae extract. The specific method comprises the following steps:
taking dried lophatherum gracile, crushing, and mixing according to the weight ratio of 1 kg: adding 80% ethanol solution into folium Bambusae at volume fraction of 7L, reflux-extracting at 60 deg.C for 2 hr, repeating extraction for 2 times, filtering, mixing filtrates, and rotary evaporating under reduced pressure to remove ethanol. And intercepting components in the extracting solution by using a membrane filtration and classification technology, and reserving the components with the molecular weight range of 100-5000 Da. Spray drying to obtain folium Bambusae extract.
Mixing the sunflower disc extract, the coix seed extract, the lophatherum gracile extract and maltodextrin in parts by weight to prepare the sunflower disc composition.
Example 2 preparation of sunflower disc composition.
The sunflower disc composition of the embodiment comprises the following raw materials in parts by weight: 80 parts of sunflower disc extract, 30 parts of coix seed extract, 5 parts of lophatherum gracile extract and 50 parts of maltodextrin. The preparation method is the same as that described in example 1.
Example 3. preparation of sunflower disc composition.
The sunflower disc composition of the embodiment comprises the following raw materials in parts by weight: 120 parts of sunflower disc extract, 15 parts of coix seed extract, 15 parts of lophatherum gracile extract and 70 parts of maltodextrin. The preparation method is the same as that described in example 1.
Example 4. preparation of sunflower disc composition.
The sunflower disc composition of the embodiment comprises the following raw materials in parts by weight: 100 parts of sunflower disc extract, 20 parts of coix seed extract, 10 parts of lophatherum gracile extract and 70 parts of maltodextrin. The preparation method comprises the following steps:
1) preparing the sunflower disc extract:
a. pulverizing Helianthus annuus L, extracting with ethanol, filtering, collecting ethanol extractive solution, extracting the residue with water, filtering, collecting water extractive solution, mixing the ethanol extractive solution and the water extractive solution, concentrating, and centrifuging to obtain supernatant;
b. and (3) carrying out enzymolysis on the supernatant by using a compound enzyme preparation, and sequentially filtering and drying the enzymolysis liquid to obtain the sunflower disc extract.
The specific method comprises the following steps:
a. pulverizing the sunflower disc, sieving with a 10-mesh sieve, and mixing according to the weight ratio of 1 kg: adding 50% ethanol solution into the sunflower disc powder at a material-liquid ratio of 5L, and extracting under reflux at 55 deg.C for 2.5 h; filtering, and collecting alcohol extract; adding water into the residue according to the ratio of 1kg raw material to 8L water, reflux-extracting at 55 deg.C for 2.5 hr for 2 times, mixing the ethanol extract and water extract, concentrating the obtained extractive mixture at 55 deg.C to fixed ratio (1 kg raw material: 1L concentrated solution), centrifuging the concentrated solution (3000 rpm, 10 min), and removing the precipitate to obtain supernatant;
b. adding complex enzyme preparation (1L concentrated solution: 10g complex enzyme) into the supernatant, and reacting at 45 deg.C for 35min while stirring. After the reaction is finished, boiling water bath is carried out for 5min, and the enzyme is inactivated; the compound enzyme preparation consists of pectinase, glucoamylase (GAL type), beta-glucanase (BGL type) and neutral xylanase, wherein the weight ratio of the pectinase: glucoamylase (GAL type): beta-glucanase (BGL type): neutral xylanase = =10:5:1:1 (mass ratio), and the enzyme is made in domestic food grade. After enzymolysis, filtering the enzymolysis liquid by using gauze of 100 meshes, and then filtering by using a microporous filter membrane (4 mu m) to remove macromolecular substances; spray drying the filtrate to obtain sunflower disc extract.
2) Preparation of coix seed extract: adding ethanol into Coicis semen, extracting, removing ethanol, subjecting the extractive solution to enzymolysis with complex enzyme preparation, sequentially filtering and drying the enzymolysis solution to obtain Coicis semen extract. The specific method comprises the following steps:
drying coix seeds, and mixing the dried coix seeds according to the weight ratio of 1 kg: adding 20% ethanol solution into Coicis semen at a material-to-liquid ratio of 5L, reflux extracting at 55 deg.C for 2.5 hr, repeating the extraction for 2 times, filtering, mixing the filtrates, and rotary evaporating under reduced pressure to remove ethanol; adding pectinase: α -amylase = 3: 1 (mass ratio) of complex enzyme, reacting at 37 ℃ for 25min, heating to inactivate the enzyme, and removing macromolecular substances with a molecular weight of more than 50 kDa by a nanofiltration device (with a pore diameter of 0.22 mu m). Spray drying the filtrate to obtain Coicis semen extract.
3) Preparing a lophatherum gracile extract: pulverizing folium Bambusae, extracting with ethanol, removing ethanol, filtering the extractive solution, and drying to obtain folium Bambusae extract. The specific method comprises the following steps:
taking dried lophatherum gracile, crushing, and mixing according to the weight ratio of 1 kg: adding 80% ethanol solution into folium Bambusae at volume fraction of 8L, reflux-extracting at 55 deg.C for 2.5h, repeating the extraction for 2 times, filtering, mixing filtrates for 3 times, and rotary evaporating under reduced pressure to remove ethanol. And intercepting components in the extracting solution by using a membrane filtration and classification technology, and reserving the components with the molecular weight range of 100-5000 Da. Spray drying to obtain folium Bambusae extract.
Mixing the sunflower disc extract, the coix seed extract, the lophatherum gracile extract and maltodextrin in parts by weight to prepare the sunflower disc composition.
Example 5 preparation of sunflower disc composition.
The sunflower disc composition of the embodiment comprises the following raw materials in parts by weight: 100 parts of sunflower disc extract, 20 parts of coix seed extract, 10 parts of lophatherum gracile extract and 70 parts of maltodextrin. The preparation method comprises the following steps:
1) preparing the sunflower disc extract:
crushing a sunflower disc, adding ethanol for ethanol extraction, filtering after extraction, collecting ethanol extract, adding water into residues for water extraction, filtering and collecting water extract, combining the ethanol extract and the water extract, concentrating the obtained extraction mixed solution, and centrifuging to obtain supernatant;
b. and (3) carrying out enzymolysis on the supernatant by using a compound enzyme preparation, and sequentially filtering and drying the enzymolysis liquid to obtain the sunflower disc extract.
The specific method comprises the following steps:
a. pulverizing the sunflower disc, sieving with a 10-mesh sieve, and mixing according to the weight ratio of 1 kg: adding 50% ethanol solution into the sunflower disc powder at a material-liquid ratio of 5L, and reflux-extracting at 62 deg.C for 1.5 h; filtering, and collecting alcohol extract; adding water into the residue according to the ratio of 1kg raw material to 8L water, reflux-extracting at 62 deg.C for 1.5 hr for 2 times, mixing the ethanol extract and water extract, concentrating the obtained extractive solution at 60 deg.C to fixed ratio (1 kg raw material: 1L concentrated solution), centrifuging the concentrated solution (3000 rpm, 10 min), and removing the precipitate to obtain supernatant;
b. adding complex enzyme preparation (1L concentrated solution: 10g complex enzyme) into the supernatant, reacting at 50 deg.C for 25min while stirring. After the reaction is finished, boiling water bath is carried out for 5min, and the enzyme is inactivated; the compound enzyme preparation consists of pectinase, glucoamylase (GAL type), beta-glucanase (BGL type) and neutral xylanase, wherein the weight ratio of the pectinase: glucoamylase (GAL type): beta-glucanase (BGL type): neutral xylanase = =10:5:1:1 (mass ratio), and the enzyme is made in domestic food grade. After enzymolysis, filtering the enzymolysis solution by using gauze of 100 meshes, and filtering by using a microporous filter membrane (4 mu m) to remove macromolecular substances; spray drying the filtrate to obtain sunflower disc extract.
2) Preparation of coix seed extract: adding ethanol into Coicis semen, extracting, removing ethanol, subjecting the extractive solution to enzymolysis with complex enzyme preparation, sequentially filtering and drying the enzymolysis solution to obtain Coicis semen extract. The specific method comprises the following steps:
drying coix seeds, and mixing the dried coix seeds according to the weight ratio of 1 kg: adding 20% ethanol into Coicis semen at a volume fraction of 6L, reflux-extracting at 62 deg.C for 1.5 hr, repeating the extraction for 2 times, filtering, mixing the filtrates, and rotary evaporating under reduced pressure to remove ethanol; adding pectinase into the extracting solution according to the proportion of adding 1 g of the compound enzyme preparation into 1kg of the raw materials: α -amylase = 3: 1 (mass ratio) of complex enzyme, reacting at 37 deg.C for 35min, heating to inactivate enzyme, removing macromolecular substances above 50 kDa with nanofiltration equipment (aperture of 0.22 μm), and spray drying the filtrate to obtain Coicis semen extract.
3) Preparing a lophatherum gracile extract: taking the lophatherum gracile, adding ethanol for ethanol extraction, removing the ethanol after extraction, and sequentially filtering and drying the extract to obtain the lophatherum gracile extract. The specific method comprises the following steps:
taking dried lophatherum gracile, and mixing the raw materials according to the weight ratio of 1 kg: adding 80% ethanol solution into folium Bambusae at volume fraction of 10L, reflux-extracting at 62 deg.C for 1.5h, repeating the extraction for 2 times, filtering, mixing filtrates, and rotary evaporating under reduced pressure to remove ethanol. And intercepting components in the extracting solution by using a membrane filtration and classification technology, and reserving the components with the molecular weight range of 100-5000 Da. Spray drying to obtain folium Bambusae extract.
Mixing the sunflower disc extract, the coix seed extract, the lophatherum gracile extract and maltodextrin in parts by weight to prepare the sunflower disc composition.
Example 6 preparation of sunflower disc solid beverage.
The sunflower disc composition can be used for preparing drinks, for example, the sunflower disc composition is granulated by a granulator (ZL 150G granulator), and packaged to prepare a solid beverage with the weight of 10g per bag. Sensory index detection is carried out on the prepared solid beverage, and the detection method comprises the following steps: taking 10g of solid beverage, placing the solid beverage in a clean white porcelain dish, and observing the color and appearance of the solid beverage by naked eyes under natural light; after being dissolved and diluted in a transparent glass beaker according to the using method on the label, the glass beaker can smell the fragrance immediately, distinguish the taste and see whether foreign matters exist at the bottom of the beaker.
TABLE 1 solid beverage sensory index test results
Example 7 sunflower disc compositions were examined for relief of acute gout.
In this example, mice were used as the study subjects, and the sunflower disc composition (example 1) was examined for its alleviation effect on acute gout in mice. For ease of administration, the sunflower disc composition was prepared as a solid drink for administration to mice.
The experimental method comprises the following steps: SD rats were randomly divided into a normal group, a model group, a low dose group, a medium dose group, a high dose group and a colchicine positive control group (0.3 mg/kg), and 6 rats were each group. The solid beverage is respectively set as the following dosage according to the low, medium and high administration groups: 200 mg/kg, 500 mg/kg and 1000 mg/kg.
The drug administration group and the control group are administered with corresponding dose of drug by intragastric administration, the normal group and the model group are administered with the same volume of physiological saline by intragastric administration, and the administration is performed 1 time and 5 days continuously at 9 am every day. On day 6, an acute gouty arthritis model was established for the model group, the administration group and the positive control group, and the specific method was: keeping an angle of about 45 degrees between an injection needle filled with 0.2 mL of sodium urate and the ankle joint back on the right side of a rat to be modeled, inserting the injection needle into the inner side of a tibial muscle bond of the rat, and injecting the injection needle into an ankle joint cavity; in the normal group, 0.2 mL of PBS buffer was injected at the same position in the same manner. The diameter (mm) of the right ankle joint of the experimental rat was measured at 7 time points of 0, 1, 2, 4, 6, 8, and 24 hours after molding, and the average value was calculated by continuously performing 3 measurements.
Degree of swelling of joint (mm) = ankle diameter after molding (mm) -ankle diameter before molding (mm). Gait was observed for each group of rats: level 0-normal walking (score 0); level 1-lightly trewing, the lower limb to be tested is slightly bent (1 minute); 2, level-moderate treplop, the tested lower limb just touches the ground (2 minutes); grade 3-the tested lower limbs are left from the ground, and the three feet are landed and walk (3 minutes).
The experimental results are as follows: after the model is made, the swelling degree of the ankle joint of the rat is gradually increased along with the prolonging of time, and the swelling degree is the most serious after 8 hours of model making; no obvious swelling is seen in the ankle joints of the rats in the normal group, and compared with the model group, the difference has statistical significance (P is less than 0.01), which indicates that the model is successfully built. Compared with the model group, the 3 dose groups of the sunflower disc solid beverage and the colchicine group have reduced ankle swelling degrees; the 3 dose groups of the sunflower disc solid beverage have no statistical significance compared with the colchicine group. The specific results are shown in figure 2.
TABLE 2 influence of sunflower disc solid drink on degree of swelling of ankle joint of rat with acute gouty arthritis
Note: compared with model groupb p<0.05；c p<0.01
After 24 h of molding, the movement of the rats in the model group is reduced, the walking posture is abnormal, the tested lower limbs touch or leave the ground lightly, the gait is that the three feet land, and the local joints are in a red and swollen state. Compared with the model group, the gaits of the 3 dose groups of the sunflower disc solid beverage and the colchicine group are obviously improved, and the difference has statistical significance. The specific results are shown in Table 3.
TABLE 3 influence of sunflower disc solid drink on gait of rat model with acute gouty arthritis
Note: compared with model groupc p<0.01
Example 8 sunflower disc compositions were examined for their effect on the relief of hyperuricemia.
In this example, mice were used as subjects, and the effects of the sunflower disc composition (example 1) on the alleviation of hyperuricemia in mice were examined. For ease of administration, the sunflower disc composition was prepared as a solid drink for administration to mice.
The experimental method comprises the following steps: male Balb-c mice were randomly divided into a normal group, a model group, a positive control allopurinol group (20 mg/kg), and a sunflower disc solid drink group (1 g/kg), each group containing 10 mice. The yeast solution was administered at a dose of 20 g/kg. On days 1 to 8, the allopurinol group and the sunflower disc solid beverage are respectively administered with corresponding solution by intragastric administration every morning; in the afternoon, each group was gavaged with yeast solution every day except for the blank group. In the experiment, the same amount of physiological saline is given to the group without gastric lavage. On days 5 to 8, 3% Potassium Oxonate (OXO) solution (300 g/kg) was intraperitoneally injected 1 h after the completion of yeast gavage, except for the blank group. The blank group was injected with an equal amount of physiological saline. 1 h after the last administration, blood is taken from orbital venous plexus of each group of mice, the plasma is stood for 30 min, centrifuged for 5min at the speed of 3000 r/min, and the supernatant is taken and stored at-80 ℃. And (4) measuring the content of the uric acid in the serum of the mouse according to the measuring method of the kit.
The experimental results are as follows: compared with the normal group, the blood uric acid value of the model group is obviously increased, which indicates that the mouse model with hyperuricemia is successfully modeled. Compared with a model group, the allopurinol and the sunflower disc solid beverage can obviously reduce the blood uric acid level of mice in a normal group, and have the function of obviously reducing the blood uric acid level of mice with hyperuricemia. The specific results are shown in Table 4.
TABLE 4 influence of sunflower disc solid drink on the blood uric acid level of hyperuricemia mice
Note: compared with the normal group# p<0.05; comparison with model group<0.05
In conclusion, the sunflower disc composition prepared by the invention has a good improvement effect on mouse gout and hyperuricemia, and can be used for preparing medicines with the effects of relieving or treating hyperuricemia, dissolving tophus and the like.
Claims (5)
1. The composite sunflower disc composition for reducing uric acid and relieving gout is characterized by comprising the following raw materials in parts by weight: 100 parts of sunflower disc extract, 20 parts of coix seed extract, 10 parts of lophatherum gracile extract and 70 parts of maltodextrin;
the preparation method of the sunflower disc composition comprises the following steps:
1) preparing the sunflower disc extract:
a. pulverizing Helianthus annuus L, extracting with ethanol, filtering, collecting ethanol extractive solution, extracting the residue with water, filtering, collecting water extractive solution, mixing the ethanol extractive solution and the water extractive solution, concentrating, and centrifuging to obtain supernatant;
b. performing enzymolysis on the supernatant by using a complex enzyme preparation, and sequentially filtering and drying enzymolysis liquid to obtain a sunflower disc extract;
2) preparation of coix seed extract: extracting Coicis semen with ethanol, removing ethanol, subjecting the extractive solution to enzymolysis with complex enzyme preparation, sequentially filtering and drying the enzymolysis solution to obtain Coicis semen extract;
3) preparing a lophatherum gracile extract: pulverizing folium Bambusae, extracting with ethanol, removing ethanol, filtering extractive solution, and drying to obtain folium Bambusae extract;
mixing the sunflower disc extract, the coix seed extract, the lophatherum gracile extract and maltodextrin in parts by weight to obtain a sunflower disc composition;
the enzymolysis in the step 1) refers to adding the complex enzyme preparation into the supernatant according to the proportion of adding 10g of the complex enzyme preparation into 1kg of raw materials, and reacting for 25-35 min at 45-60 ℃; the compound enzyme preparation consists of pectinase, glucoamylase, beta-glucanase and neutral xylanase, wherein the mass ratio of the enzymes is as follows: and (3) pectinase: glucoamylase: beta-glucanase: neutral xylanase = (8-12): 3-7): 0.8-1.2);
the alcohol extraction in the step 2) is performed according to the ratio of 1 kg: (3-7) adding 20% ethanol solution into Coicis semen at a volume fraction of 20-L, and reflux-extracting at 55-62 deg.C for 1.5-2.5 h; the enzymolysis refers to adding the complex enzyme preparation into the extract according to the proportion of adding 0.5-1 g of the complex enzyme preparation into 1kg of the raw materials, and reacting for 25-35 min at 37 ℃; the compound enzyme preparation consists of pectinase and alpha-amylase, wherein the mass ratio of the enzymes is as follows: and (3) pectinase: α -amylase = 3: 1.
2. the composition of claim 1, wherein the alcohol extraction in step 1) is performed according to a weight ratio of 1 kg: adding 50% ethanol solution into the sunflower disc powder at a material-liquid ratio of 5L, and reflux-extracting at 55-62 deg.C for 1.5-2.5 h; the water extraction is to add water into the residue according to the proportion of adding 8L of water into 1kg of raw materials, and extract for 1.5-2.5h at 55-62 ℃.
3. The composition of claim 1, wherein the alcohol extraction in step 3) is performed according to a ratio of 1 kg: (6-10) adding 80% ethanol solution into the crushed lophatherum gracile leaves according to the material-liquid ratio, and performing reflux extraction at 55-62 ℃ for 1.5-2.5 h.
4. The composition as claimed in claim 1), wherein the filtration in step 1) b is performed by filtering the enzymatic hydrolysate through 100 mesh gauze, and then filtering through a microporous membrane with a pore size of 4 μm; the filtration in the step 2) refers to the nanofiltration filtration of the enzymolysis liquid; the filtration in the step 3) is a membrane filtration and classification method, and components with the molecular weight of 100-5000 Da are intercepted; the drying in steps 1) -3) is spray drying.
5. The use of the sunflower disc composition of claim 1 in the preparation of a medicament for the relief or treatment of gout, hyperuricemia.
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| CN110327434A (en) * | 2019-08-14 | 2019-10-15 | 吉林省特医食品生物科技有限公司 | A kind of Chinese medicine composition that treating gout, compound Chinese medicinal preparation and preparation method thereof |
| CN110478470A (en) * | 2019-08-28 | 2019-11-22 | 长春中医药大学 | A kind of anti-gout composition of the small-molecular peptides containing sunflower disk and preparation method thereof |
| CN111053846A (en) * | 2019-12-23 | 2020-04-24 | 武汉轻工大学 | Pseudobulbus cremastrae seu pleiones composition, and preparation method and application thereof |
| CN111838673A (en) * | 2020-07-27 | 2020-10-30 | 吴伟 | Sunflower pollen extract and preparation method and application thereof |
| CN113082173A (en) * | 2021-03-24 | 2021-07-09 | 贵州宏创农业产业开发有限公司 | Coix seed extract and application thereof in preparation of medicament for treating septicemia |
| CN114027432A (en) * | 2021-12-17 | 2022-02-11 | 广东长昊药业股份有限公司 | Uric acid-removing gout-preventing sunflower disc solid beverage and preparation method and application thereof |
| CN114377050A (en) * | 2022-02-15 | 2022-04-22 | 吉林大学 | Anti-gout composition containing sunflower disc micromolecules |
| CN114947124A (en) * | 2022-04-22 | 2022-08-30 | 北京恒生药业有限公司 | Solid composition with gout relieving effect and preparation method thereof |
| CN115444139B (en) * | 2022-09-15 | 2023-08-08 | 草根知本集团有限公司 | Application of beta-glucan or extract thereof in preparation of medicines for improving hyperuricemia |
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| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
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| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
| CN108522931A (en) * | 2018-03-30 | 2018-09-14 | 张延强 | A kind of energy acid discharge deacidification cementing stone adjusts the sunflower disk solid beverage of acid-base balance |
| CN109045224A (en) * | 2018-10-29 | 2018-12-21 | 吉林大学 | A kind of sunflower disk semen coicis compound and the purposes in treatment hepatic injury |
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