CN109833443A - It is a kind of to reduce uric acid, compound sunflower disk composition of alleviation gout and the preparation method and application thereof - Google Patents
It is a kind of to reduce uric acid, compound sunflower disk composition of alleviation gout and the preparation method and application thereof Download PDFInfo
- Publication number
- CN109833443A CN109833443A CN201910294302.7A CN201910294302A CN109833443A CN 109833443 A CN109833443 A CN 109833443A CN 201910294302 A CN201910294302 A CN 201910294302A CN 109833443 A CN109833443 A CN 109833443A
- Authority
- CN
- China
- Prior art keywords
- extract
- sunflower disk
- preparation
- parts
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
It is a kind of to reduce uric acid, compound sunflower disk composition of alleviation gout and the preparation method and application thereof, belong to technical field of functional food.The problems such as in order to solve big existing gout treatment drug side effect, complicated component, the present invention passes through the methods of extraction, enzymatic hydrolysis, molecular weight retention, optimize the preparation method of sunflower disk extract, Semen Coicis extract and Herba Lophatheri extract, the effective components such as the extract obtained active sugar complexes rich in small-molecular-weight, flavones, alkaloid.The composition and preparation method thereof that the present invention also provides a kind of to be made of sunflower disk extract 80-120 parts by weight, Semen Coicis extract 10-30 parts by weight, Herba Lophatheri extract 5-15 parts by weight and maltodextrin 50-70 parts by weight, the present invention can be used for improving the exploitation of the health care product and drug of gout and/or hyperuricemia.
Description
Technical field
The invention belongs to technical field of functional food, and in particular to a kind of compound sunflower disk for reducing uric acid, alleviating gout
Composition and the preparation method and application thereof.
Background technique
Gout be by monosodium urate salt (MSU) deposition caused by crystal correlation arthropathy, with purine metabolic disturbance and
(or) hyperuricemia caused by underexcretion is directly related, mainly include acute attack arthritis, tophus formed,
Tophaceous chornic arthritis, urate nephropathy and uric acid lithangiuria, severe one may occur in which joint deformity and renal insufficiency.
Gout is often with the performance such as Central obesity, hyperlipidemia, hypertension, diabetes B and cardiovascular disease.
In acute attack stage, by drug therapy or gout and hyperuricemia can be alleviated, but after acute stage symptom control
If not taking certain comprehensive preventive health measures, it is difficult to restore normal automatically.Treatment acute gout or hyperuricemia are more at present
Chemicals are used, although there is certain effect, side effect is big, it is very serious to GI irritation, through normal after patient's medication
Phenomena such as will appear diarrhea, nausea, sour regurgitation, seriously affects the quality of life of patient.Some health medicines are claimed to have and alleviate pain
Wind reduces the effect of uric acid level, but constituent is excessively complicated, or even the formula having reaches 10 kinds or more, quality mark when production
Standard is difficult to control, and functional component is not clear enough.And its effect also much is confirmed without zoopery or human trial.
Summary of the invention
The problems such as in order to solve big existing gout treatment drug side effect, complicated component, the present invention provides a kind of sunflowers
Disk composition, the composition are composed of the following raw materials in parts by weight: sunflower disk extract 80-120 parts by weight, Semen Coicis extract
10-30 parts by weight, Herba Lophatheri extract 5-15 parts by weight and maltodextrin 50-70 parts by weight.
Preferably, the composition is composed of the following raw materials in parts by weight: 100 parts of sunflower disk extract, Semen Coicis extract
20 parts, 10 parts of Herba Lophatheri extract and 70 parts of maltodextrin.
The present invention also provides the preparation methods of above-mentioned sunflower disk composition, include the following steps:
1) preparation of sunflower disk extract:
A. sunflower disk is crushed, ethyl alcohol is added and carries out alcohol extracting, is filtered after extraction, collects alcohol extract, in residue plus water into
Row water mentions, and aqueous extract is collected by filtration, and merges alcohol extract and aqueous extract, and gained extracts the concentrated rear centrifugation of mixed liquor, obtains
To supernatant；
B. supernatant is digested through complex enzyme formulation, and enzymolysis liquid is successively filtered, is dried, and obtains sunflower disk extract；
2) preparation of Semen Coicis extract: taking semen coicis, and ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol, extracting solution are removed after extraction
It is digested through complex enzyme formulation, enzymolysis liquid is successively filtered, is dried, and Semen Coicis extract is obtained；
3) preparation of Herba Lophatheri extract: taking lophatherum gracile, and ethyl alcohol is added after crushing and carries out alcohol extracting, removes ethyl alcohol after extraction,
Extracting solution is successively filtered, is dried, and Herba Lophatheri extract is obtained；
Above-mentioned sunflower disk extract, Semen Coicis extract, Herba Lophatheri extract are mixed by weight with maltodextrin, i.e.,
Obtain sunflower disk composition.
It further limits, alcohol extracting described in step 1) refers to the solid-liquid ratio according to 1kg:5L, is added into sunflower disk powder
The ethanol solution that volume fraction is 50%, 55-62 DEG C of refluxing extraction 1.5-2.5h；The water mentions, and refers to and adds 8L according to 1kg raw material
The ratio of water adds water, 55-62 DEG C of refluxing extraction 1.5-2.5h into residue.
It further limits, is digested described in step 1), refer to the ratio for adding 10g complex enzyme formulation according to 1kg raw material, to
Complex enzyme formulation, 45-50 DEG C of reaction 25-35min are added in supernatant；The complex enzyme formulation by pectase, glucoamylase,
1,4 beta-glucanase and neutral xylanase composition, wherein the mass ratio of each enzyme are as follows: pectase: glucoamylase: 1,4 beta-glucanase:
Neutral xylanase=(8-12): (3-7): (0.8-1.2): (0.8-1.2).
Further limit, the step 2) alcohol extracting refers to according to 1kg:(3-7) solid-liquid ratio of L, it is added into semen coicis
The ethanol solution that volume fraction is 20%, 55-62 DEG C of refluxing extraction 1.5-2.5h；The enzymatic hydrolysis refers to and adds according to 1kg raw material
Complex enzyme formulation, 37 DEG C of reaction 25-35min are added into extracting solution for the ratio of 0.5-1g complex enzyme formulation；The complex enzyme system
Agent is made of pectase and alpha-amylase, wherein the mass ratio of each enzyme are as follows: pectase: alpha-amylase=3:1.
Further limit, the step 3) alcohol extracting refers to according to 1kg:(6-10) solid-liquid ratio of L, to smashed henon bamboo
The ethanol solution that volume fraction is 80%, 55-62 DEG C of refluxing extraction 1.5-2.5h are added in leaf.
It further limits, filtering described in step 1) refers to first by enzymolysis liquid through 100 mesh filtered through gauze, then via hole diameter
For 4 μm of filtering with microporous membrane；Filtering, which refers to, described in step 2) filters enzymolysis liquid through nanofiltration；It is filtered into described in step 3)
Film filtering hierarchical method, molecular cut off are the ingredient of 100~5000Da；Step 1) -3) described in drying be spray drying.
The present invention also provides a kind of sunflower disk solid beverages, contain above-mentioned sunflower disk composition.
Sunflower disk composition of the present invention can be used for preparing alleviation or treat gout, hyperuricemia health care product or
Drug.
Beneficial effect
The present invention provides it is a kind of composition it is relatively easy, functional component is clear, function animal experiments prove that and without pair make
Sunflower disk composition.
Sunflower disk composition of the present invention is all made of 60 DEG C or so low during the preparation process, extracting and being concentrated
Temperature can effectively prevent the structure and texture property of carbohydrate and polyphenols in high temperature water extract.
Mouse experiment shows sunflower disk composition prepared by the present invention, and gout symptom can be effectively relieved, significant to reduce
Hyperuricemia mouse serum uric acid level can be used for alleviating gout, reduce the exploitation of antihyperuricemic disease drug.
The present invention establishes the preparation method of sunflower disk extract, Semen Coicis extract and Herba Lophatheri extract, and provides
It is the solid beverage that main component has treatment gout and hyperuricemia that more than one, which state 3 kinds of raw materials, has no phase on the market
Same product.
Specific embodiment
Raw material used herein can be bought by commercialization approach and be obtained, for example, pectase, glucoamylase, β-
Dextranase, neutral xylanase pectase and alpha-amylase are bought in Cangzhou Xia Sheng biotechnology of enzymes Co., Ltd.
The preparation of 1. sunflower disk composition of embodiment.
Sunflower disk composition described in the present embodiment is made of the raw material of following parts by weight: 100 parts of sunflower disk extract, the heart of a lotus seed
20 parts of seed of jog's tears extract, 10 parts of Herba Lophatheri extract and 70 parts of maltodextrin.The preparation method is as follows:
1) preparation of sunflower disk extract:
A. sunflower disk is crushed, ethyl alcohol is added and carries out alcohol extracting, is filtered after extraction, collects alcohol extract, in residue plus water into
Row water mentions, and aqueous extract is collected by filtration, and merges alcohol extract and aqueous extract, and gained extracts the concentrated rear centrifugation of mixed liquor, obtains
To supernatant；
B. supernatant is digested through complex enzyme formulation, and enzymolysis liquid is successively filtered, is dried, and obtains sunflower disk extract.
Method particularly includes:
A. sunflower disk is beaten into powder, crosses 10 meshes, according to the solid-liquid ratio of 1kg:5L, volume fraction, which is added, into sunflower disk powder is
50% ethanol solution, 60 DEG C of refluxing extraction 2h；Alcohol extract is collected in filtering；The ratio for adding 8L water according to 1kg raw material, to residual
Add water in slag, 60 DEG C of low temperature are extracted 2 hours, are extracted 2 times altogether, and alcohol extract and aqueous extract are merged, and gained extracts mixed liquor 60
After DEG C being concentrated into fixed proportion (1kg raw material: 1L concentrate), concentrate is centrifuged (3000rpm, 10min), abandons precipitating, in acquisition
Clear liquid；
B. complex enzyme formulation (1L concentrate: 10g complex enzyme) is added into supernatant, 47 DEG C of reaction 30min are during which continuous
Stirring.Boiling water bath 5min after completion of the reaction, by enzyme-deactivating；The complex enzyme formulation by pectase, glucoamylase (GAL type),
1,4 beta-glucanase (BGL type) and neutral xylanase form, wherein pectase: glucoamylase (GAL type): 1,4 beta-glucanase
(BGL type): neutral xylanase==10:5:1:1 (mass ratio), the enzyme are domestic food-grade.After enzymatic hydrolysis, enzymolysis liquid is passed through
100 mesh filtered through gauze are filtered using (4 μm) of miillpore filter, remove macromolecular substances；After gained filtrate is spray-dried, obtain
Sunflower disk extract.
2) preparation of Semen Coicis extract: taking semen coicis, and ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol, extracting solution are removed after extraction
It is digested through complex enzyme formulation, enzymolysis liquid is successively filtered, is dried, and Semen Coicis extract is obtained.Method particularly includes:
By dry semen coicis, according to the solid-liquid ratio of 1kg:4L, it is that 20% ethyl alcohol is molten that volume fraction is added into semen coicis
Liquid, 60 DEG C of refluxing extraction 2h repeat to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, remove ethyl alcohol；According to 1kg
Raw material adds the ratio of 0.6g complex enzyme formulation, and pectase is added into extracting solution: alpha-amylase=3:1 (mass ratio) it is compound
Enzyme-deactivating is removed the macromolecular of 50kDa or more through nanofiltration equipment (aperture is 0.22 μm) by enzyme, 37 DEG C of reaction 30min, heating
Substance, gained filtrate is spray-dried, obtains Semen Coicis extract.
3) preparation of Herba Lophatheri extract: taking lophatherum gracile, and ethyl alcohol is added after crushing and carries out alcohol extracting, removes ethyl alcohol after extraction,
Extracting solution is successively filtered, is dried, and Herba Lophatheri extract is obtained.Method particularly includes:
Dry lophatherum gracile is taken, is crushed, according to the solid-liquid ratio of 1kg:7L, it is 80% second that volume fraction is added into lophatherum gracile
Alcoholic solution, 60 DEG C of refluxing extraction 2h repeat to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, remove ethyl alcohol.It utilizes
Film filtering hierarchical technology, retains the ingredient in extracting solution, leaves and takes the ingredient that molecular weight ranges are 100~5000Da.Spray
After mist is dry, Herba Lophatheri extract is obtained.
After above-mentioned sunflower disk extract, Semen Coicis extract, Herba Lophatheri extract are mixed by weight with maltodextrin,
Prepare sunflower disk composition.
The preparation of 2. sunflower disk composition of embodiment.
Sunflower disk composition described in the present embodiment is made of the raw material of following parts by weight: 80 parts of sunflower disk extract, the heart of a lotus seed
30 parts of seed of jog's tears extract, 5 parts of Herba Lophatheri extract and 50 parts of maltodextrin.It is described that the preparation method is the same as that of Example 1.
The preparation of 3. sunflower disk composition of embodiment.
Sunflower disk composition described in the present embodiment is made of the raw material of following parts by weight: 120 parts of sunflower disk extract, the heart of a lotus seed
15 parts of seed of jog's tears extract, 15 parts of Herba Lophatheri extract and 70 parts of maltodextrin.It is described that the preparation method is the same as that of Example 1.
The preparation of 4. sunflower disk composition of embodiment.
Sunflower disk composition described in the present embodiment is made of the raw material of following parts by weight: 100 parts of sunflower disk extract, the heart of a lotus seed
20 parts of seed of jog's tears extract, 10 parts of Herba Lophatheri extract and 70 parts of maltodextrin.The preparation method is as follows:
1) preparation of sunflower disk extract:
A. sunflower disk is crushed, ethyl alcohol is added and carries out alcohol extracting, is filtered after extraction, collects alcohol extract, in residue plus water into
Row water mentions, and aqueous extract is collected by filtration, and merges alcohol extract and aqueous extract, and gained extracts the concentrated rear centrifugation of mixed liquor, obtains
To supernatant；
B. supernatant is digested through complex enzyme formulation, and enzymolysis liquid is successively filtered, is dried, and obtains sunflower disk extract.
Method particularly includes:
A. sunflower disk is beaten into powder, crosses 10 meshes, according to the solid-liquid ratio of 1kg:5L, volume fraction, which is added, into sunflower disk powder is
50% ethanol solution, 55 DEG C of refluxing extraction 2.5h；Alcohol extract is collected in filtering；The ratio for adding 8L water according to 1kg raw material, to
In residue plus water, 55 DEG C of refluxing extraction 2.5h of low temperature are extracted 2 times altogether, merge alcohol extract and aqueous extract, gained extracts mixing
After 55 DEG C of liquid are concentrated into fixed proportion (1kg raw material: 1L concentrate), concentrate is centrifuged (3000rpm, 10min), abandons precipitating, obtains
Obtain supernatant；
B. complex enzyme formulation (1L concentrate: 10g complex enzyme) is added into supernatant, 45 DEG C of reaction 35min are during which continuous
Stirring.Boiling water bath 5min after completion of the reaction, by enzyme-deactivating；The complex enzyme formulation by pectase, glucoamylase (GAL type),
1,4 beta-glucanase (BGL type) and neutral xylanase form, wherein pectase: glucoamylase (GAL type): 1,4 beta-glucanase
(BGL type): neutral xylanase==10:5:1:1 (mass ratio), the enzyme are domestic food-grade.After enzymatic hydrolysis, enzymolysis liquid is passed through
100 mesh filtered through gauze recycle (4 μm) of miillpore filter filterings, remove macromolecular substances；After gained filtrate is spray-dried, obtain
Obtain sunflower disk extract.
2) preparation of Semen Coicis extract: by semen coicis, ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol, extracting solution are removed after extraction
It is digested through complex enzyme formulation, enzymolysis liquid is successively filtered, is dried, and Semen Coicis extract is obtained.Method particularly includes:
By dry semen coicis, according to the solid-liquid ratio of 1kg:5L, it is that 20% ethyl alcohol is molten that volume fraction is added into semen coicis
Liquid, 55 DEG C of refluxing extraction 2.5h repeat to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, remove ethyl alcohol；According to
1kg raw material adds the ratio of 0.8g complex enzyme formulation, and pectase is added into extracting solution: alpha-amylase=3:1 (mass ratio) is answered
Enzyme-deactivating is removed big point of 50kDa or more through nanofiltration equipment (aperture is 0.22 μm) by synthase, 37 DEG C of reaction 25min, heating
Sub- substance.Gained filtrate is spray-dried, obtains Semen Coicis extract.
3) preparation of Herba Lophatheri extract: taking lophatherum gracile, and ethyl alcohol is added after crushing and carries out alcohol extracting, removes ethyl alcohol after extraction,
Extracting solution is successively filtered, is dried, and Herba Lophatheri extract is obtained.Method particularly includes:
Dry lophatherum gracile is taken, is crushed, according to the solid-liquid ratio of 1kg:8L, it is 80% second that volume fraction is added into lophatherum gracile
Alcoholic solution, 55 DEG C of refluxing extraction 2.5h repeat to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, remove ethyl alcohol.Benefit
With film filtering hierarchical technology, the ingredient in extracting solution is retained, leaves and takes the ingredient that molecular weight ranges are 100~5000Da.
After spray drying, Herba Lophatheri extract is obtained.
After above-mentioned sunflower disk extract, Semen Coicis extract, Herba Lophatheri extract are mixed by weight with maltodextrin,
Prepare sunflower disk composition.
The preparation of 5. sunflower disk composition of embodiment.
Sunflower disk composition described in the present embodiment is made of the raw material of following parts by weight: 100 parts of sunflower disk extract, the heart of a lotus seed
20 parts of seed of jog's tears extract, 10 parts of Herba Lophatheri extract and 70 parts of maltodextrin.The preparation method is as follows:
1) preparation of sunflower disk extract:
A. sunflower disk is crushed, ethyl alcohol is added and carries out alcohol extracting, is filtered after extraction, collects alcohol extract, in residue plus water into
Row water mentions, and aqueous extract is collected by filtration, and merges alcohol extract and aqueous extract, and gained extracts the concentrated rear centrifugation of mixed liquor, obtains
To supernatant；
B. supernatant is digested through complex enzyme formulation, and enzymolysis liquid is successively filtered, is dried, and obtains sunflower disk extract.
Method particularly includes:
A. sunflower disk is beaten into powder, crosses 10 meshes, according to the solid-liquid ratio of 1kg:5L, volume fraction, which is added, into sunflower disk powder is
50% ethanol solution, 62 DEG C of refluxing extraction 1.5h；Alcohol extract is collected in filtering；The ratio for adding 8L water according to 1kg raw material, to
In residue plus water, 62 DEG C of refluxing extraction 1.5h of low temperature are extracted 2 times altogether, merge alcohol extract and aqueous extract, gained extracts mixing
After 60 DEG C of liquid are concentrated into fixed proportion (1kg raw material: 1L concentrate), concentrate is centrifuged (3000rpm, 10min), abandons precipitating, obtains
Obtain supernatant；
B. complex enzyme formulation (1L concentrate: 10g complex enzyme) is added into supernatant, 50 DEG C of reaction 25min are during which continuous
Stirring.Boiling water bath 5min after completion of the reaction, by enzyme-deactivating；The complex enzyme formulation by pectase, glucoamylase (GAL type),
1,4 beta-glucanase (BGL type) and neutral xylanase form, wherein pectase: glucoamylase (GAL type): 1,4 beta-glucanase
(BGL type): neutral xylanase==10:5:1:1 (mass ratio), the enzyme are domestic food-grade.After enzymatic hydrolysis, enzymolysis liquid is passed through
100 mesh filtered through gauze are filtered using (4 μm) of miillpore filter, remove macromolecular substances；After gained filtrate is spray-dried, obtain
Sunflower disk extract.
2) preparation of Semen Coicis extract: by semen coicis, ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol, extracting solution are removed after extraction
It is digested through complex enzyme formulation, enzymolysis liquid is successively filtered, is dried, and Semen Coicis extract is obtained.Method particularly includes:
By dry semen coicis, according to the solid-liquid ratio of 1kg:6L, it is 20% ethyl alcohol 62 that volume fraction is added into semen coicis
DEG C refluxing extraction 1.5h repeats to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, removes ethyl alcohol；According to 1kg raw material
The ratio for adding 1g complex enzyme formulation, pectase is added into extracting solution: the complex enzyme of alpha-amylase=3:1 (mass ratio), and 37 DEG C
35min is reacted, enzyme-deactivating is removed the macromolecular substances of 50kDa or more, institute through nanofiltration equipment (aperture is 0.22 μm) by heating
It is spray-dried to obtain filtrate, obtains Semen Coicis extract.
3) preparation of Herba Lophatheri extract: taking lophatherum gracile, and ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol, extracting solution are removed after extraction
It successively filtered, be dried, obtain Herba Lophatheri extract.Method particularly includes:
Dry lophatherum gracile is taken, according to the solid-liquid ratio of 1kg:10L, it is that 80% ethyl alcohol is molten that volume fraction is added into lophatherum gracile
Liquid, 62 DEG C of refluxing extraction 1.5h repeat to extract 2 times, and filtering merges 3 filtrates, depressurizes rotary evaporation, remove ethyl alcohol.Utilize film
Filtering hierarchical technology retains the ingredient in extracting solution, leaves and takes the ingredient that molecular weight ranges are 100~5000Da.It is spraying
After drying, Herba Lophatheri extract is obtained.
After above-mentioned sunflower disk extract, Semen Coicis extract, Herba Lophatheri extract are mixed by weight with maltodextrin,
Prepare sunflower disk composition.
The preparation of 6. sunflower disk solid beverage of embodiment.
Sunflower disk composition of the present invention can be used for preparing drink, such as by sunflower disk composition through granulator
The solid beverage of 10g/ packet is made in (ZL150G granulator) granulation, packaging.Organoleptic indicator's inspection is carried out to prepared solid beverage
Survey, detection method are as follows: take solid beverage 10g, be placed in clean white porcelain dish, detect by an unaided eye under natural light its color and
Mode of appearance；By application method described on label after rushing molten dilution in transparent glass beaker, its perfume is smelt immediately, distinguishes its taste
Taste, seeing bottom of the beaker, whether there is or not alien materials.
1 solid beverage organoleptic indicator's testing result of table
Embodiment 7. investigates relaxation effect of the sunflower disk composition to acute gout.
The present embodiment investigates sunflower disk composition (by taking embodiment 1 as an example) to chmice acute pain using mouse as research object
The relaxation effect of wind.In order to facilitate administration, solid beverage form is made in sunflower disk composition and gives mouse.
Experimental method: SD rat is randomly divided into normal group, model group, low dosage administration group, middle dosage administration group, high agent
Amount administration group and colchicin positive controls (0.3mg/kg), every group 6.Solid beverage is by low, medium and high administration group
Dosage is set to respectively: 200mg/kg, 500mg/kg and 1000mg/kg.
Administration group and control group give the drug gastric infusion of matched doses, and normal group and model group then give same volume
Physiological saline stomach-filling, per daily at 9 in morning gastric infusion 1 time, continuous 5d.6th day, to model group, administration group and the positive
Control group establishes acute gouty arthritis model, method particularly includes: by equipped with 0.2mL Monosodium urate injection needle with need modeling big
Ankle-joint back on the right side of mouse keeps about 45° angle, is inserted into the inside of its shin bone tendon forceps, and injection ankle-joint is intracavitary；Normal group is then pressed
Same method, in the PBS buffer solution of same position injection 0.2mL.After modeling 0,1,2,4,6,8, for 24 hours, totally 7 time
Point measures the right ankle-joint diameter (mm) of experimental rat, is carried out continuously 3 measurements, calculates average value.
The ankle-joint diameter (mm) before ankle-joint diameter (mm)-modeling after Articular swelling (mm)=modeling.To each group
The gait of rat is observed: 0 grade-normal walking (0 point)；1 grade-slight cross mountains is gone, and tested lower limb are slightly bent (1 point)；2 grades-
Moderate, which is crossed mountains, goes, and tested lower limb have just touched ground (2 points)；3 grades-severe is crossed mountains row, and tested lower limb leave ground, and tripodia lands walking (3
Point).
Experimental result: after modeling, with the extension of time, the swelling degree of rat ankle joint gradually aggravates, 8h after modeling
Swelling degree is the most serious；Normal rats ankle-joint has no that obvious tumefaction is compared with model group, and difference has statistical significance
(P < 0.01) shows model building success.Compared with model group, 3 dosage groups of sunflower disk solid beverage and colchicin group,
The swelling degree of ankle-joint is declined；3 dosage groups of sunflower disk solid beverage are compared with colchicin group, and difference is without statistics
Learn meaning.Concrete outcome is shown in 2.
Influence of the 2 sunflower disk solid beverage of table to acute gouty arthritis ankle swelling in rat degree
Note: compared with model groupbp<0.05；c p<0.01
After modeling for 24 hours, model group rats activity is reduced, and walking posture is abnormal, and tested lower limb touch or leave ground, are in
Red and swollen state is presented in the gait that tripodia lands, local joint.Compared with model group, 3 dosage groups of sunflower disk solid beverage and autumn
The gait of tazettine group improves significantly, and difference has statistical significance.Concrete outcome is shown in Table 3.
Influence of the 3 sunflower disk solid beverage of table to acute gouty arthritis in rats gait
Note: compared with model groupc p<0.01
Embodiment 8. investigates relaxation effect of the sunflower disk composition to hyperuricemia.
The present embodiment investigates sunflower disk composition (by taking embodiment 1 as an example) to mouse high lithemia using mouse as research object
The relaxation effect of mass formed by blood stasis.In order to facilitate administration, solid beverage form is made in sunflower disk composition and gives mouse.
Experimental method: male Balb-c mouse is randomly divided into normal group, model group, positive control drug Allopurinol group
(20mg/kg), sunflower disk solid beverage group (1g/kg), every group 10.The dosage of yeast soln is 20g/kg.It arrives within 1st day
8th day, corresponding solution was given in every morning, Allopurinol group and sunflower disk solid beverage each group stomach-filling；Remove blank group every afternoon
Outside, yeast soln is given in the equal stomach-filling of each group.The group that stomach-filling is not required in experimentation then gives same amount of normal saline.5th day extremely
8th day, 1h after yeast stomach-filling, in addition to blank group, 3% Oteracil Potassium (OXO) solution (300g/ was injected intraperitoneally in each group
kg).Blank group injects same amount of normal saline.1h after last time is administered, each group mouse orbit veniplex take blood, and blood plasma is stood
After 30min, the speed of 3000r/min is centrifuged 5min, takes supernatant, is placed in -80 DEG C of preservations.It is carried out according to the measuring method of kit
The measurement of mice serum uric acid content.
Experimental result: compared with normal group, model group blood uric acid value is dramatically increased, and illustrates that antihyperuricemic disease mouse model is built
Mould success.Compared with model group, Allopurinol and sunflower disk solid beverage can significantly reduce mouse blood uric acid value, and lower than normal
Group mouse serum uric acid level, tool are significantly reduced the effect of hyperuricemia mouse serum uric acid level.Concrete outcome is shown in Table 4.
Influence of the 4 sunflower disk solid beverage of table to hyperuricemia mouse blood uric acid value
Note: compared with normal group#p<0.05；Compared with model group*p<0.05
To sum up, the sunflower disk composition that the present invention prepares has mouse gout and hyperuricemia good
Good improvement result, can be used for preparing has the health care product or medicine alleviating or treating hyperuricemia and dissolve tophus and other effects
Product.
Claims (10)
1. a kind of sunflower disk composition, which is characterized in that the composition is composed of the following raw materials in parts by weight: sunflower disk extract
80-120 parts by weight, Semen Coicis extract 10-30 parts by weight, Herba Lophatheri extract 5-15 parts by weight and maltodextrin 50-70 weight
Measure part.
2. sunflower disk composition according to claim 1, which is characterized in that the composition by following parts by weight raw material group
At: 100 parts of sunflower disk extract, 20 parts of Semen Coicis extract, 10 parts of Herba Lophatheri extract and 70 parts of maltodextrin.
3. the preparation method of sunflower disk composition of any of claims 1 or 2, which comprises the steps of:
1) preparation of sunflower disk extract:
A. sunflower disk is crushed, ethyl alcohol is added and carries out alcohol extracting, is filtered after extraction, collected alcohol extract, add water to carry out water in residue
It mentions, aqueous extract is collected by filtration, merge alcohol extract and aqueous extract, gained extracts the concentrated rear centrifugation of mixed liquor, obtains
Clear liquid；
B. supernatant is digested through complex enzyme formulation, and enzymolysis liquid is successively filtered, is dried, and obtains sunflower disk extract；
2) preparation of Semen Coicis extract: taking semen coicis, and ethyl alcohol is added and carries out alcohol extracting, ethyl alcohol is removed after extraction, extracting solution is through multiple
Synthase preparation enzymatic hydrolysis, enzymolysis liquid are successively filtered, are dried, and Semen Coicis extract is obtained；
3) preparation of Herba Lophatheri extract: taking lophatherum gracile, and ethyl alcohol is added after crushing and carries out alcohol extracting, ethyl alcohol is removed after extraction, extracts
Liquid is successively filtered, is dried, and Herba Lophatheri extract is obtained；
Above-mentioned sunflower disk extract, Semen Coicis extract, Herba Lophatheri extract are mixed by weight with maltodextrin to get certain herbaceous plants with big flowers
Floral disc composition.
4. preparation method according to claim 3, which is characterized in that alcohol extracting described in step 1) refers to according to 1kg:5L
Solid-liquid ratio, into sunflower disk powder be added volume fraction be 50% ethanol solution, 55-62 DEG C of refluxing extraction 1.5-2.5h；Institute
It states water to mention, refers to the ratio for adding 8L water according to 1kg raw material, water is added into residue, in 55-62 DEG C of extraction 1.5-2.5h.
5. preparation method according to claim 3, which is characterized in that digest, refer to according to 1kg raw material described in step 1)
Complex enzyme formulation, 45-60 DEG C of reaction 25-35min are added into supernatant for the ratio for adding 10g complex enzyme formulation；The complex enzyme
Preparation is made of pectase, glucoamylase, 1,4 beta-glucanase and neutral xylanase, wherein the mass ratio of each enzyme are as follows: pectin
Enzyme: glucoamylase: 1,4 beta-glucanase: neutral xylanase=(8-12): (3-7): (0.8-1.2): (0.8-1.2).
6. preparation method according to claim 3, which is characterized in that the step 2) alcohol extracting refers to according to 1kg:(3-7) L
Solid-liquid ratio, into semen coicis be added volume fraction be 20% ethanol solution, 55-62 DEG C of refluxing extraction 1.5-2.5h；It is described
Enzymatic hydrolysis, refers to the ratio for adding 0.5-1g complex enzyme formulation according to 1kg raw material, and complex enzyme formulation is added into extracting solution, and 37 DEG C anti-
Answer 25-35min；The complex enzyme formulation is made of pectase and alpha-amylase, wherein the mass ratio of each enzyme are as follows: pectase: α-
Amylase=3:1.
7. preparation method according to claim 3, which is characterized in that the step 3) alcohol extracting refers to according to 1kg:(6-10)
The ethanol solution that volume fraction is 80%, 55-62 DEG C of refluxing extraction 1.5- are added into smashed lophatherum gracile for the solid-liquid ratio of L
2.5h。
8. preparation method according to claim 3, which is characterized in that filtering, which refers to, described in step 1) first passes through enzymolysis liquid
100 mesh filtered through gauze, the filtering with microporous membrane that then via hole diameter is 4 μm；Filtering described in step 2) refers to enzymolysis liquid through receiving
Filtration filter；Film filtering hierarchical method is filtered into described in step 3), molecular cut off is the ingredient of 100~5000Da；Step 1)-
3) drying described in is spray drying.
9. a kind of sunflower disk solid beverage, which is characterized in that contain sunflower disk composition of any of claims 1 or 2.
10. the health care product of gout, hyperuricemia is alleviated or treated to sunflower disk composition of any of claims 1 or 2 in preparation
Or the application in drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910294302.7A CN109833443B (en) | 2019-04-12 | 2019-04-12 | Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910294302.7A CN109833443B (en) | 2019-04-12 | 2019-04-12 | Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109833443A true CN109833443A (en) | 2019-06-04 |
| CN109833443B CN109833443B (en) | 2021-07-27 |
Family
ID=66887020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910294302.7A Active CN109833443B (en) | 2019-04-12 | 2019-04-12 | Composite sunflower disc composition for reducing uric acid and relieving gout as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109833443B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327434A (en) * | 2019-08-14 | 2019-10-15 | 吉林省特医食品生物科技有限公司 | A kind of Chinese medicine composition that treating gout, compound Chinese medicinal preparation and preparation method thereof |
| CN110478470A (en) * | 2019-08-28 | 2019-11-22 | 长春中医药大学 | A kind of anti-gout composition of the small-molecular peptides containing sunflower disk and preparation method thereof |
| CN111053846A (en) * | 2019-12-23 | 2020-04-24 | 武汉轻工大学 | Pseudobulbus cremastrae seu pleiones composition, and preparation method and application thereof |
| CN111838673A (en) * | 2020-07-27 | 2020-10-30 | 吴伟 | Sunflower pollen extract and preparation method and application thereof |
| CN113082173A (en) * | 2021-03-24 | 2021-07-09 | 贵州宏创农业产业开发有限公司 | Coix seed extract and application thereof in preparation of medicament for treating septicemia |
| CN114027432A (en) * | 2021-12-17 | 2022-02-11 | 广东长昊药业股份有限公司 | Uric acid-removing gout-preventing sunflower disc solid beverage and preparation method and application thereof |
| CN114377050A (en) * | 2022-02-15 | 2022-04-22 | 吉林大学 | Anti-gout composition containing sunflower disc micromolecules |
| CN114947124A (en) * | 2022-04-22 | 2022-08-30 | 北京恒生药业有限公司 | Solid composition with gout relieving effect and preparation method thereof |
| CN115444139A (en) * | 2022-09-15 | 2022-12-09 | 草根知本集团有限公司 | Application of beta-glucan or extract thereof in preparation of food or medicine for improving hyperuricemia |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
| CN108522931A (en) * | 2018-03-30 | 2018-09-14 | 张延强 | A kind of energy acid discharge deacidification cementing stone adjusts the sunflower disk solid beverage of acid-base balance |
| CN109045224A (en) * | 2018-10-29 | 2018-12-21 | 吉林大学 | A kind of sunflower disk semen coicis compound and the purposes in treatment hepatic injury |
-
2019
- 2019-04-12 CN CN201910294302.7A patent/CN109833443B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
| CN108522931A (en) * | 2018-03-30 | 2018-09-14 | 张延强 | A kind of energy acid discharge deacidification cementing stone adjusts the sunflower disk solid beverage of acid-base balance |
| CN109045224A (en) * | 2018-10-29 | 2018-12-21 | 吉林大学 | A kind of sunflower disk semen coicis compound and the purposes in treatment hepatic injury |
Non-Patent Citations (1)
| Title |
|---|
| 魏庆芳等主编: "《生活妙招防治痛风》", 31 January 2013, 人民军医出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327434A (en) * | 2019-08-14 | 2019-10-15 | 吉林省特医食品生物科技有限公司 | A kind of Chinese medicine composition that treating gout, compound Chinese medicinal preparation and preparation method thereof |
| CN110478470A (en) * | 2019-08-28 | 2019-11-22 | 长春中医药大学 | A kind of anti-gout composition of the small-molecular peptides containing sunflower disk and preparation method thereof |
| CN111053846A (en) * | 2019-12-23 | 2020-04-24 | 武汉轻工大学 | Pseudobulbus cremastrae seu pleiones composition, and preparation method and application thereof |
| CN111838673A (en) * | 2020-07-27 | 2020-10-30 | 吴伟 | Sunflower pollen extract and preparation method and application thereof |
| CN113082173A (en) * | 2021-03-24 | 2021-07-09 | 贵州宏创农业产业开发有限公司 | Coix seed extract and application thereof in preparation of medicament for treating septicemia |
| CN114027432A (en) * | 2021-12-17 | 2022-02-11 | 广东长昊药业股份有限公司 | Uric acid-removing gout-preventing sunflower disc solid beverage and preparation method and application thereof |
| CN114377050A (en) * | 2022-02-15 | 2022-04-22 | 吉林大学 | Anti-gout composition containing sunflower disc micromolecules |
| CN114947124A (en) * | 2022-04-22 | 2022-08-30 | 北京恒生药业有限公司 | Solid composition with gout relieving effect and preparation method thereof |
| CN115444139A (en) * | 2022-09-15 | 2022-12-09 | 草根知本集团有限公司 | Application of beta-glucan or extract thereof in preparation of food or medicine for improving hyperuricemia |
| CN115444139B (en) * | 2022-09-15 | 2023-08-08 | 草根知本集团有限公司 | Application of beta-glucan or extract thereof in preparation of medicines for improving hyperuricemia |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109833443B (en) | 2021-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109833443A (en) | It is a kind of to reduce uric acid, compound sunflower disk composition of alleviation gout and the preparation method and application thereof | |
| CN103719880B (en) | Preparation method of high-activity purple sweet potato dietary fiber | |
| CN104256584B (en) | A kind of Moringa ferment and preparation method thereof | |
| CN104757564B (en) | A kind of method utilizing Pericarppium arachidis hypogaeae to prepare dietary fiber | |
| CN100417665C (en) | Method for preparing pollen pini polyose, pollen pipe polyose and its use in medicine | |
| CN109938135A (en) | A kind of cassia seed tea-drinking and preparation method thereof | |
| CN102225970B (en) | Extraction method of polysaccharide from potentilla discolor | |
| CN101664180B (en) | Health-care nutritional complexing agent with health effect and preparation method thereof | |
| CN107875196A (en) | Complex enzyme, extract solution system, medicament, extract and preparation method thereof, application | |
| CN108174951A (en) | A kind of mulberry leaf sealwort tea preparation method of auxiliary hyperglycemic | |
| CN106360741A (en) | Sugar-reducing plant composite protein powder and preparation method thereof | |
| CN107114801B (en) | High-content oat dietary fiber preparation and preparation method thereof | |
| CN108686113A (en) | A kind of composition and preparation method thereof for alleviating three high diseases shape | |
| CN107712170A (en) | A kind of preparation method and applications of soluble dietary fiber | |
| CN102232561B (en) | Chinese wolfberry cellulose capsule and preparation method thereof | |
| CN101658619A (en) | Method for extracting aloe arborescens miller whole-leaf juice based on complex enzyme treatment technology | |
| CN106389535A (en) | Application of industrial hemp cannaboid in preparing gout treating medicines | |
| CN109717426A (en) | A kind of hypoglycemia healthcare food and preparation method thereof | |
| CN108936701A (en) | A kind of preparation method of vegetable seed meal dietary fiber | |
| CN113197983A (en) | Sunflower disc-derived composition for treating gout and preparation method and application thereof | |
| CN106905383A (en) | A kind of hypoglycemic use glucoside extract and its manufacture method | |
| CN1297353A (en) | Hypoylycemic agents | |
| CN106879774A (en) | A kind of tea with auxiliary hyperglycemic effect and preparation method thereof | |
| CN107047852A (en) | Preparation method of multifunctional burdock health-care tea pulp | |
| CN113652461A (en) | Preparation method of polygonatum sibiricum phytosterol polysaccharide protein and active product thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |