CN101658619A - Method for extracting aloe arborescens miller whole-leaf juice based on complex enzyme treatment technology - Google Patents
Method for extracting aloe arborescens miller whole-leaf juice based on complex enzyme treatment technology Download PDFInfo
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- CN101658619A CN101658619A CN200910112575A CN200910112575A CN101658619A CN 101658619 A CN101658619 A CN 101658619A CN 200910112575 A CN200910112575 A CN 200910112575A CN 200910112575 A CN200910112575 A CN 200910112575A CN 101658619 A CN101658619 A CN 101658619A
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Abstract
The invention relates to a method for extracting aloe arborescens miller whole-leaf juice, in particular to a method for extracting aloe arborescens miller whole-leaf juice based on a complex enzyme treatment technology. The technical scheme is as follows: a method for extracting aloe arborescens miller whole-leaf juice, which takes aloe arborescens miller as raw material, adopts the complex enzyme treatment technology, and comprises the steps of: pretreating aloe arborescens miller leaf, adding enzyme for treatment and obtaining a finished product of aloe arborescens miller whole-leaf juice.The added enzyme is complex enzyme which is prepared according to the following proportions: 0.08% of cellulase, 0.09% of refined pectinase, 0.06% of protease and 0.05% of a-amylase which are added toaloe juice, the pH value is adjusted to be 4.2-5.0, and enzymolysis is conducted at the enzymolysis temperature of 45-50 DEG C. By utilizing the method, the technical problems that the aloe arborescens miller juice is in the form of a multi-factor unstable system, and has easy precipitation and browning due to abundant protein, pectin, polysaccharide and polyphenols in the aloe arborescens millerjuice can be effectively solved; and the extraction ratio is above 65%, the barbaloin is 5.5 mg/100 ml, the membrane filtration flux/kg.(h.m2)<-1> is 3490, the light transmittance/% is 90.2, and thesensory evaluation is 93.
Description
Technical field
The present invention relates to a kind of extracting method of Aloe arborescens var. natalensis whole-leaf juice, relate to a kind of specifically based on the extracting method of compound enzyme treatment technology to the Aloe arborescens var. natalensis whole-leaf juice.
Technical background
Aloe belongs to the Liliaceae herbaceos perennial, and its effective ingredient mainly contains protein, aminoacid, various trace elements, aloin, aloe-emodin, tinctura aloes, Aloe flavor tincture and aloe-emodin, organized enzyme and multivitamin.The application of Aloe aspect medical, it can promote wound healing, the treatment wound; Digestion, breathing, the many commonly encountered diseases of blood circulation there is curative effect preferably, as preferable to common gastritis, enteritis, cancer, diabetes, constipation, oral ulcer effect.Aspect beauty treatment, Aloe can be wrinkle resistant, relaxes pachylosis, eliminates or minimizing acne, freckle etc.Aspect health care, Aloe beverage, Aloe wine, Aloe tea and aloe moisture-keeping frost all have the Weight-reducing health effect.In a word, Aloe arborescens var. natalensis can provide the pure natural equalizing nutrient for human body, and 90% is ionic condition, easily digests and assimilates for human body.Aloe belongs to alkalescence food, can keep the health acid-base balance; Its cellulosic can combine with cholesterol in intestinal, promotes cholesterol to descend; Cellulosic and Fructus Gleditsia can promote intestinal peristalsis promoting, favourable defecation.The aloe product of China belongs to primary product mostly, and scientific and technological content is not high, from product structure, skin protection, cosmetics are more, food and medicine are less, and the Aloe industry still is in the junior stage, and estimate that China will face the severe challenge that external aloe product dominates the market in the 2-3.Therefore, the research and development of reinforcement aloe product are significant.
In the processing of Aloe juice, Aloe vulgaris has more product, but is not to be full juice, and is mesophyll juice (peeling extracting juice), and the highly purified juice product of high stability is also rarely arranged simultaneously.Aspect Aloe arborescens var. natalensis, studies have shown that, Aloe arborescens var. natalensis juice rich in proteins, pectin, polysaccharide and Polyphenols, and be multifactor Unstable Systems, easily precipitation, easily brown stain, for Aloe vulgaris, composition is more complicated, also more unstable, therefore adopt the routine techniques measure often to be difficult to prove effective.The Aloe arborescens var. natalensis juice poor stability of processing, is prone to precipitation at easily brown stain, and the kind that is considered to make the Aloe juice.
Summary of the invention
For overcoming the deficiency of existing method, the present invention is a raw material with Aloe arborescens var. natalensis (A.arborescens.Mill), adopt compound enzyme technical finesse technology, provide a kind of by the pretreatment of Aloe arborescens var. natalensis blade, add that complex enzyme zymohydrolysis is handled, the extracting method of the Aloe arborescens var. natalensis whole-leaf juice of finished product.Utilize this method, solve the Aloe arborescens var. natalensis juice effectively because of rich in proteins, pectin, polysaccharide and Polyphenols, and be multifactor Unstable Systems, easily the technical barrier of precipitation, easy brown stain.The Aloe arborescens var. natalensis whole-leaf juice of this investigative technique explained hereafter has the characteristic of high stability, high-purity, high active ingredient and clear, and that properties of product reach is medical, the specification requirement of edible and liquid make-up, shelf-life＞12 month.The whole-leaf juice product that extracts, medical, the edible specification requirement that reaches liquid make-up that performance reaches, help to promote China's Aloe industry development, enrich the people's living substance, improve the capacity to earn foreign exchange through exports of China's medicinal plants, promote China's Aloe arborescens var. natalensis process technology to enter advanced international standard.
The present invention is achieved through the following technical solutions:
1, leave pretreatment
Gather the aloe leaf of growth about 2 years, remove the blade tip base portion, and scab, the part of rotting,, drain and weigh with the visible dirt of tap water cleaning blade; And metaphyll adopts 70 ℃-85 ℃ hot water to carry out burn, utilizes general juice extractor to squeeze the juice at last, and it is standby to extract the Aloe juice.Aloe leaf is taken from Fujian Prosperous Biotechnology Development Co., Ltd
2, complex enzyme zymohydrolysis is handled
Prepare compound enzyme in following ratio: cellulase 0.08%, refining pectase 0.09%, proteinase-10 .06%, a-amylase 0.05% (weight after all draining) in full leaf.Its enzymolysis process condition is: pH value 4.2-5.0, hydrolysis temperature 45--50 ℃, enzymolysis time 2.0-3.0 hour, the extraction ratio of Aloe arborescens var. natalensis whole-leaf juice, barbaloin content, membrane filtration flux all improved a lot.
3, post processing
Whole-leaf juice behind the enzymolysis is heated to 80-95 ℃ and keep carrying out in 0.5 hour enzyme denaturing, then adopts conventional membrane filter system to filter roguing.Adopt 138 ℃ to handle sterilization in 5 seconds down at last, fill is the whole-leaf juice finished product.
Cellulase, refining pectase, protease, a-amylase are Denmark Novo Nordisk Co.,Ltd and produce.
The specific embodiment
Embodiment 1
1, leave pretreatment
Gather the aloe leaf of growth about 2 years, remove the blade tip base portion, reach scab, rotten part,, drain and obtain 300 kilograms clean aloe leaf with the visible dirt of tap water cleaning blade; And metaphyll adopts 75 ℃ hot water to carry out burn, utilizes general juice extractor to squeeze the juice at last, and it is standby to extract the Aloe juice.Aloe leaf is taken from Fujian Prosperous Biotechnology Development Co., Ltd permanently happy Aloe base.
2, complex enzyme zymohydrolysis is handled
Prepare compound enzyme in following ratio: 24 kilograms of cellulase, 27 kilograms of refining pectases, 18 kilograms in protease, 15 kilograms of a-amylase join behind the mixing and carry out enzymolysis in the Aloe juice.Its enzymatic hydrolysis condition is: pH value 5.0,50 ℃ of hydrolysis temperatures, enzymolysis time 2.0 hours.Cellulase, refining pectase, protease, a-amylase are Denmark Novo Nordisk Co.,Ltd and produce.
3, post processing
Whole-leaf juice behind the enzymolysis is heated to 85 ℃ and keep carrying out in 0.5 hour enzyme denaturing, then adopts conventional membrane filter system to filter roguing.Adopt 138 ℃ to handle sterilization in 5 seconds down at last, fill is the whole-leaf juice finished product.
Finished product is measured in the following manner:
The assay method of barbaloin: by the assay method of the company standard Q/CLJY001-2006 of Fujian Prosperous Biotechnology Development Co., Ltd.
Light transmittance/%: use 722 spectrophotometric determinations.
Sensory evaluation: the expert evaluation method is adopted in sensory evaluation, forms evaluation group with 10 people, adopts the hundred-mark system point system, respectively its color and luster, tissue morphology, taste abnormal smells from the patient is given a mark.Remove a best result, remove a minimum branch, get eight average mark through round up the integer branch, full marks are 100 minutes.
Concrete outcome is as follows: extraction ratio (%) is 68, and barbaloin (mg/100ml) is 5.6, membrane filtration flux/kg (hm2)
-1Be 3490, light transmittance/% is 90.2, and sensory evaluation is 93.
Embodiment 2
1, leave pretreatment
Gather the aloe leaf of growth about 2 years, remove the blade tip base portion, reach scab, rotten part,, drain and obtain 300 kilograms clean aloe leaf with the visible dirt of tap water cleaning blade; And metaphyll adopts 70 ℃ hot water to carry out burn, utilizes general juice extractor to squeeze the juice at last, and it is standby to extract the Aloe juice.Aloe leaf is taken from Fujian Prosperous Biotechnology Development Co., Ltd permanently happy Aloe base.
2, complex enzyme zymohydrolysis is handled
Prepare compound enzyme in following ratio: 24 kilograms of cellulase, 27 kilograms of refining pectases, 18 kilograms in protease, 15 kilograms of a-amylase join behind the mixing and carry out enzymolysis in the Aloe juice.Its enzymatic hydrolysis condition is: pH value 4.2,45 ℃ of hydrolysis temperatures, enzymolysis time 3.0 hours.Cellulase, refining pectase, protease, a-amylase are Denmark Novo Nordisk Co.,Ltd and produce.
3, post processing
Whole-leaf juice behind the enzymolysis is heated to 95 ℃ and keep carrying out in 0.5 hour enzyme denaturing, then adopts conventional membrane filter system to filter roguing.Adopt 138 ℃ to handle sterilization in 5 seconds down at last, fill is the whole-leaf juice finished product.
Finished product is measured in the following manner:
The assay method of barbaloin: by the assay method of the company standard Q/CLJY001-2006 of Fujian Prosperous Biotechnology Development Co., Ltd.
Light transmittance/%: use 722 spectrophotometric determinations.
Sensory evaluation: the expert evaluation method is adopted in sensory evaluation, forms evaluation group with 10 people, adopts the hundred-mark system point system, respectively its color and luster, tissue morphology, taste abnormal smells from the patient is given a mark.Remove a best result, remove a minimum branch, get eight average mark through round up the integer branch, full marks are 100 minutes.
Concrete outcome is as follows: extraction ratio (%) is 69, and barbaloin (mg/100ml) is 5.7, membrane filtration flux/kg (hm2)
-1Be 3510, light transmittance/%90.3, sensory evaluation are 92.
Claims (3)
1, a kind of based on the extracting method of compound enzyme treatment technology to the Aloe arborescens var. natalensis whole-leaf juice, it is the aloe leaf of gathering growth about 2 years, through the pretreatment of Aloe arborescens var. natalensis blade, the extracting method that adds the Aloe arborescens var. natalensis whole-leaf juice of complex enzyme zymohydrolysis processing and finished product, when it is characterized in that adding the complex enzyme zymohydrolysis processing weight after draining in full leaf, prepare compound enzyme in following ratio: cellulase 0.08%, refining pectase 0.09%, proteinase-10 .06%, a-amylase 0.05%, and join the Aloe juice, adjust pH value, hydrolysis temperature carries out 2.0-3.0 hour enzymolysis.
2, according to claim 1 based on the extracting method of compound enzyme treatment technology to the Aloe arborescens var. natalensis whole-leaf juice, it is characterized in that pH value is 4.2-5.0.
3, according to claim 1 based on the extracting method of compound enzyme treatment technology to the Aloe arborescens var. natalensis whole-leaf juice, it is characterized in that hydrolysis temperature 45--50 ℃.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869330A (en) * | 2010-06-23 | 2010-10-27 | 天人果汁集团股份有限公司 | Process for producing concentrated clear apricot juice |
| CN102813096A (en) * | 2012-09-07 | 2012-12-12 | 云南万绿生物股份有限公司 | Method for removing pigment and barbaloin in aloe barbadensis juice |
| CN103609802A (en) * | 2013-12-03 | 2014-03-05 | 江苏省农业科学院 | Preparation method of aloe herbal tea compound health beverage and product thereof |
| CN106456525A (en) * | 2014-04-17 | 2017-02-22 | 荷兰联合利华有限公司 | Aloe vera extract for personal care compositions |
| CN109393459A (en) * | 2018-10-18 | 2019-03-01 | 安徽济生元药业有限公司 | A kind of aloe capsule of energy defaecation |
-
2009
- 2009-09-27 CN CN200910112575A patent/CN101658619A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869330A (en) * | 2010-06-23 | 2010-10-27 | 天人果汁集团股份有限公司 | Process for producing concentrated clear apricot juice |
| CN102813096A (en) * | 2012-09-07 | 2012-12-12 | 云南万绿生物股份有限公司 | Method for removing pigment and barbaloin in aloe barbadensis juice |
| CN103609802A (en) * | 2013-12-03 | 2014-03-05 | 江苏省农业科学院 | Preparation method of aloe herbal tea compound health beverage and product thereof |
| CN103609802B (en) * | 2013-12-03 | 2015-10-07 | 江苏省农业科学院 | Preparation method of a kind of aloe herbal tea compound health beverage and products thereof |
| CN106456525A (en) * | 2014-04-17 | 2017-02-22 | 荷兰联合利华有限公司 | Aloe vera extract for personal care compositions |
| CN106456525B (en) * | 2014-04-17 | 2019-12-03 | 荷兰联合利华有限公司 | Aloe extract for personal care composition |
| CN109393459A (en) * | 2018-10-18 | 2019-03-01 | 安徽济生元药业有限公司 | A kind of aloe capsule of energy defaecation |
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Open date: 20100303 |