CN106905383A - A kind of hypoglycemic use glucoside extract and its manufacture method - Google Patents
A kind of hypoglycemic use glucoside extract and its manufacture method Download PDFInfo
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- CN106905383A CN106905383A CN201710209011.4A CN201710209011A CN106905383A CN 106905383 A CN106905383 A CN 106905383A CN 201710209011 A CN201710209011 A CN 201710209011A CN 106905383 A CN106905383 A CN 106905383A
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- 239000000284 extract Substances 0.000 title claims abstract description 61
- 150000008131 glucosides Chemical class 0.000 title claims abstract description 35
- 229930182478 glucoside Natural products 0.000 title claims abstract description 34
- 230000002218 hypoglycaemic effect Effects 0.000 title claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 57
- ILRCGYURZSFMEG-RKQHYHRCSA-N Salidroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-RKQHYHRCSA-N 0.000 claims abstract description 46
- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 claims abstract description 23
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 claims abstract description 23
- 229930185803 charantin Natural products 0.000 claims abstract description 23
- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 claims abstract description 23
- 229940052490 naringin Drugs 0.000 claims abstract description 23
- 229930019673 naringin Natural products 0.000 claims abstract description 23
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229930182493 triterpene saponin Natural products 0.000 claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims description 164
- 238000005238 degreasing Methods 0.000 claims description 73
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 56
- 230000004913 activation Effects 0.000 claims description 42
- 244000131316 Panax pseudoginseng Species 0.000 claims description 39
- 239000002994 raw material Substances 0.000 claims description 39
- 235000003181 Panax pseudoginseng Nutrition 0.000 claims description 38
- 240000000560 Citrus x paradisi Species 0.000 claims description 36
- 244000302512 Momordica charantia Species 0.000 claims description 36
- 235000009811 Momordica charantia Nutrition 0.000 claims description 36
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 32
- 241001165494 Rhodiola Species 0.000 claims description 32
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- 238000001914 filtration Methods 0.000 claims description 20
- 239000007787 solid Substances 0.000 claims description 20
- 238000005237 degreasing agent Methods 0.000 claims description 16
- 239000013527 degreasing agent Substances 0.000 claims description 16
- 238000010304 firing Methods 0.000 claims description 16
- 239000003208 petroleum Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 230000018044 dehydration Effects 0.000 claims description 12
- 238000006297 dehydration reaction Methods 0.000 claims description 12
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 235000009812 Momordica cochinchinensis Nutrition 0.000 claims description 8
- 235000018365 Momordica dioica Nutrition 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 239000003480 eluent Substances 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 7
- 244000276331 Citrus maxima Species 0.000 claims description 4
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- 239000007788 liquid Substances 0.000 claims description 2
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- 229930182490 saponin Natural products 0.000 description 4
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- 241000196324 Embryophyta Species 0.000 description 3
- 239000003472 antidiabetic agent Substances 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000000767 anti-ulcer Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
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- 201000001421 hyperglycemia Diseases 0.000 description 2
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- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical group N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
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- -1 terpene saponin Chemical class 0.000 description 2
- 235000007586 terpenes Nutrition 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- 240000001879 Digitalis lutea Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
- 208000035150 Hypercholesterolemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036592 analgesia Effects 0.000 description 1
- 230000000146 antalgic effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002019 anti-mutation Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 230000002567 autonomic effect Effects 0.000 description 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
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- 230000036772 blood pressure Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
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- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000000496 cardiotonic agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000037111 immune power Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- LPMXVESGRSUGHW-HBYQJFLCSA-N ouabain Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1C[C@@]2(O)CC[C@H]3[C@@]4(O)CC[C@H](C=5COC(=O)C=5)[C@@]4(C)C[C@@H](O)[C@@H]3[C@@]2(CO)[C@H](O)C1 LPMXVESGRSUGHW-HBYQJFLCSA-N 0.000 description 1
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- 229930002534 steroid glycoside Natural products 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7032—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a polyol, i.e. compounds having two or more free or esterified hydroxy groups, including the hydroxy group involved in the glycosidic linkage, e.g. monoglucosyldiacylglycerides, lactobionic acid, gangliosides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
- A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
- A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/18—Acyclic radicals, substituted by carbocyclic rings
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
- C07H15/256—Polyterpene radicals
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- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
- C07H17/07—Benzo[b]pyran-4-ones
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- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a kind of hypoglycemic use glucoside extract and its manufacture method, the hypoglycemic glucoside extract is made up of the composition of following weight portion：25 32 parts of Charantin, 12 15 parts of rhodioloside, 58 parts of total triterpene saponin, 15 20 parts of naringin, 8 10 parts of Paeoniflorin, six steps etc. the extraction of its manufacture method including Charantin, the extraction of rhodioloside, the extraction of total triterpene saponin, the extraction of naringin, the extraction of Paeoniflorin, the manufacture of target glucoside extract.It is of the invention it is hypoglycemic with glucoside extract and its manufacture method it is applied widely, effective ingredient is more, effective ingredient has complementary functions mutually promote, extraction efficiency it is high, obtain product purity it is high, be easily achieved.
Description
Technical field
The present invention relates to biomedicine field, more particularly to a kind of hypoglycemic use glucoside extract and its manufacture method.
Background technology
Glucoside extract, also known as glycoside extracts, glycocide, it is combined into by sugar and nonsugar.The general character of glucoside exists
The part of sugar, there are different physiologically actives in different types of glucoside unit, with many functions.As contained cardiac stimulant in digitalis folia
The cardiac glycoside of effect, in ginseng containing tonifying Qi, promote the production of body fluid, the panaxoside of sedative action etc..As carbohydrate, amino acid, protein,
Enzyme, organic acid, grease, wax, resin, pigment, inorganic matter etc., respectively with special physiological function, wherein being much clinically
Important drugs.The legislation category and concept and service condition of comprehensive various countries, plant extracts this concept can be by institute of various countries
Receive and approve, be also to propagate herbal medicine in the general general character expression way in various countries.The export amount of Chinese Plants extract early in
The export amount for just having exceeded Chinese patent drug in 1999.In American-European countries, plant extracts and its product（Autonomic drug or food are supplemented
Agent）There are extensive market prospects, have evolved into a new industry for nearly 8,000,000,000 dollars of annual sales amount.The plant extract of China
Thing is generally the product for belonging to intermediate, and current purposes widely, is mainly used in medicine, health food, tobacco, cosmetic
Raw material or auxiliary material of product etc..The species of the material plant for extracting is also very more, and the plant variety of industrial abstract is entered at present
More than 300 kinds.
In the Patents applied at home, patent《Yuenkanin total saponins prepare the purposes of hypoglycemic drug》（Application number：
201110239059.2, publication date：2011-11-30）Disclose a kind of yuenkanin total saponins and prepare hypoglycemic drug, what it was used
It is the first alcohol extracting degreasing method that alcohol is sieved again again, is provided without ultrasonic extraction and enzymatic isolation method, extraction efficiency is relatively low, obtains product pure
Degree is relatively low；Further, since only a kind of active ingredient, it is impossible to suitable for many situations, also mutually promote effect without complementary, function compared with
Limitation.Patent《Watt careless Pentacyclic triterpene saponins compounds prepare the purposes of hypoglycemic drug》（Application number：
201310627408.7, publication date：2014-02-26）Disclose a kind of watt of careless Pentacyclic triterpene saponins compounds, but its public affairs
The chemical formula of effective ingredient is opened, undisclosed extracting mode is realized difficult.
The content of the invention
For drawbacks described above present in prior art, the present invention is intended to provide a kind of applied widely, effective ingredient it is many,
Effective ingredient have complementary functions mutually promote, extraction efficiency is high, obtain product purity high, the hypoglycemic use glucoside extract that is easily achieved and
Its manufacture method.
To achieve these goals, the present invention uses following technical scheme：A kind of manufacture of hypoglycemic use glucoside extract
Method, comprises the following steps：
1）The extraction of Charantin
1. raw material are prepared：Balsam pear, toasts, during baking after removing the peel the balsam pear, remove flesh, remove seed under 40 DEG C of -60 DEG C of environment
Between be 2h-3h；Dewatered bitter gourd is done and shreds and be milled into powder by baking after terminating, and after the sieve using 40-100 mesh, is sieved
What is gone out is as the bitter melon powder of raw material；
2. the bitter melon powder that 1. step obtains is placed in Soxhlet extractor and is used after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing bitter melon powder；
3. the degreasing bitter melon powder for 2. step being obtained is put into the absolute ethyl alcohol of its 50-60 times of quality multiple；Then will add
The absolute ethyl alcohol of degreasing bitter melon powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the absolute ethyl alcohol that degreasing bitter melon powder will be added is heated to 60 DEG C -70 DEG C, is then carried out with the power of 350W-450W
Ultrasonic activation is extracted, and ultrasonic activation extraction time is 35min-55min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Charantin needed for obtaining, the Charantin is in brownish-yellow powder shape；
2）The extraction of rhodioloside
1. raw material are prepared：Rhodiola root, dries by rhodiola root removal aerial part and after cleaning under 40 DEG C of -60 DEG C of environment
Roasting, baking time is 2h-3h；Dehydration rhodiola root is done and shreds and be milled into powder by baking after terminating, using the sieve of 80-100 mesh
After sieving, that is sifted out is as the rhodiola root dry powder of raw material；
2. 20% ethanol water that quality is 15-20 times of the rhodiola root dry powder that 1. step obtains is prepared, 20% ethanol is water-soluble
The pH value of liquid is allocated to 7.8-8.5；The rhodiola root dry powder that 1. step is obtained is put into 20% deployed ethanol water；So
20% ethanol water that rhodiola root dry powder will be added afterwards is positioned in the ultrasonic activation machine for being provided with firing equipment；
3. 20% ethanol water that rhodiola root dry powder will be added is heated to 40 DEG C -60 DEG C, then with the power of 500W-600W
Ultrasonic activation extraction is carried out, ultrasonic activation extraction time is 50min-60min；
4. the extract solution for 3. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, rhodioloside needed for obtaining, the rhodioloside is in light brown or white powder；
3）The extraction of total triterpene saponin
1. raw material are prepared：Pseudo-ginseng, toasts after the pseudo-ginseng is cleaned under 40 DEG C of -60 DEG C of environment, and baking time is 2h-3h；
Dehydration pseudo-ginseng is done and shreds and be milled into powder by baking after terminating, and after the sieve using 60-80 mesh, that is sifted out is conduct
The pseudo-ginseng dry powder of raw material；
2. the pseudo-ginseng dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing pseudo-ginseng dry powder；
3. 75% ethanol water that quality is 30-40 times of the degreasing pseudo-ginseng dry powder that 2. step obtains is prepared, by 75% ethanol water
The pH value of solution is allocated to 7.5-8；The degreasing pseudo-ginseng dry powder that 2. step is obtained is put into 75% deployed ethanol water；
Then 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is heated to 40 DEG C -60 DEG C, then with the work(of 800W-1000W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 8min-15min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, total triterpene saponin needed for obtaining, the total triterpene saponin is in light brown or white powder；
4）The extraction of naringin
1. raw material are prepared：Grapefruit peel, toasts, baking time after the grapefruit peel is cleaned under 40 DEG C of -60 DEG C of environment
It is 2h-3h；Dewatered grape shaddock ped is done and shreds and be milled into powder by baking after terminating, and after the sieve using 60-80 mesh, is sieved
What is gone out is as the grapefruit peel dry powder of raw material；
2. the grapefruit peel dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to be carried out at degreasing for degreasing agent
Air-dried after reason, that is, obtain degreasing grapefruit peel dry powder；
3. 90% ethanol water that quality is 30-40 times of the degreasing grapefruit peel dry powder that 2. step obtains is prepared, by 90% second
The pH value of alcohol solution is allocated to 9-10；The degreasing grapefruit peel dry powder that 2. step is obtained is put into 90% deployed ethanol water
In solution；Then 90% ethanol water that degreasing grapefruit peel dry powder will be added is positioned over the ultrasound for being provided with firing equipment
In ripple bobbing machine；
4. 90% ethanol water that degreasing grapefruit peel dry powder will be added is heated to 85 DEG C -90 DEG C, then with 500W-600W
Power carry out ultrasonic activation extraction, ultrasonic activation extraction time is 1.5h-2h；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, naringin needed for obtaining, the naringin is in faint yellow or off-white powder shape；
5）The extraction of Paeoniflorin
1. raw material are prepared：Peony Root, toasts after the Peony Root is cleaned under 40 DEG C of -60 DEG C of environment, and baking time is 2h-
3h；Baking the dry chopping of dehydration Peony Root and will be milled into powder after terminating, after the sieve using 60-80 mesh, sifted out i.e.
It is as the Peony Root dry powder of raw material；
2. the Peony Root dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing Peony Root dry powder；
3. it is what 2. step obtained to prepare the cellulase and quality that quality is the degreasing Peony Root dry powder 1%-3% that 2. step obtains
The pure water of 8-10 times of degreasing Peony Root dry powder；Degreasing Peony Root dry powder, cellulase, pure water are mixed and stirred for uniformly；
4. the mixed liquor that 3. step obtains is heated to 30 DEG C -50 DEG C, soaking time 3h-4h；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and macroporous absorbent resin is then put into surplus solution
Post, keeps 10min-15min；
6. the large pore resin absorption column for having adsorbed extract that 5. step obtains is cleaned using pure water, then again using anhydrous
Ethanol elution, obtains eluent；
7. the eluent that 6. step obtains is placed under closed environment and spontaneously dried, that is, Paeoniflorin needed for obtaining, the Paeoniflorin
In sepia or off-white powder shape；
6）Target glucoside extract is produced
By 1）~5）The various glucoside extracts for obtaining uniformly are mixed by following weight portion：Charantin 25-32 parts, rhodioloside 12-
15 parts, 5-8 parts of total triterpene saponin, naringin 15-20 parts, Paeoniflorin 8-10 parts, that is, hypoglycemic use glucoside extract needed for obtaining.
The hypoglycemic of above-mentioned manufacture method manufacture uses glucoside extract, the composition composition of following weight portion：Charantin 25-32
Part, rhodioloside 12-15 parts, 5-8 parts, naringin 15-20 parts, Paeoniflorin 8-10 parts of total triterpene saponin.
Compared with the prior art, it is of the invention as a result of such scheme, with advantages below：Employ various different
Glucoside extract, wherein：Charantin can be steady hypoglycemic, can improve insulin function, repairs β cells, mitigates and II type of control
Hyperglycemia complication, with adjusting hypertension, high fat of blood, high cholesterol, protects effect of cardiovascular and cerebrovascular, can adjust immune
Power, enhances metabolism；Rhodioloside can protect angiocarpy, promote blood glucose balance, anti-aging, radioresistance；Total triterpene saponin energy
Protection nervous centralis, balances endocrine（Become phase control blood sugar concentration）, lifting immunity, improve body energy；Naringin is resistant to
Scorching, antiviral, anticancer, anti-mutation, antiallergy, antiulcer, analgesia, hypoglycemic, hypotensive activity, can hypercholesterolemia, reduction
The formation of thrombus；Paeoniflorin can reduce blood sugar, expansion of blood vessels, antalgic and sedative, anti-inflammatory antiulcer, antipyretic spasmolysis, diuresis；To sum up institute
State, five kinds of effective ingredients play the role of certain hypoglycemic or control blood sugar concentration, it is outer also respectively with protection blood vessel, protection
The functions such as nervous system, balance endocrine, lifting immunity, lowering blood pressure and blood fat, anti-aging, radioresistance.It is well known that hyperglycaemia
Be easily caused diabetes, and diabetes mainly by complication cause a disease it is lethal die, therefore treatment diabetes can not be only hypoglycemic, carries
Rise human body each side function equally play the role of it is important, by the rational proportion obtained by test of many times can obtain institute it is active
The optimum proportioning of effect, therefore applied widely, effective ingredient of the invention is more, effective ingredient has complementary functions mutually rush.Due to what is used
All it is that the alcohol extracting affine to glucoside extract or alcohol-water mixture are extracted, then aids in ultrasonic wave extraction or enzymolysis and extraction,
And whole process is all carried out under closed, clean environment, extraction efficiency is high, it is high to obtain product purity；It is whole due to disclosing
Technological process and formula, are easily achieved Manufacturing Method of Products of the invention.
Specific embodiment
Embodiment 1：
A kind of hypoglycemic composition for using glucoside extract, following weight portion is constituted：25 parts of Charantin, 12 parts of rhodioloside, total three
5 parts of terpene saponin, 15 parts of naringin, 8 parts of Paeoniflorin.
The manufacture method of above-mentioned hypoglycemic use glucoside extract, comprises the following steps：
1）The extraction of Charantin
1. raw material are prepared：Balsam pear, toasts after removing the peel the balsam pear, remove flesh, remove seed under 40 DEG C of environment, and baking time is
2h；Dewatered bitter gourd is done and shreds and be milled into powder by baking after terminating, and after the sieve using 40 mesh, that is sifted out is conduct
The bitter melon powder of raw material；
2. the bitter melon powder that 1. step obtains is placed in Soxhlet extractor and is used after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing bitter melon powder；
3. the degreasing bitter melon powder for 2. step being obtained is put into the absolute ethyl alcohol of 50 times of its quality multiple；Then will add de-
The absolute ethyl alcohol of fat bitter melon powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the absolute ethyl alcohol that degreasing bitter melon powder will be added is heated to 60 DEG C, then carries out ultrasonic activation with the power of 350W
Extract, ultrasonic activation extraction time is 35min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Charantin needed for obtaining, the Charantin is in brownish-yellow powder shape；
2）The extraction of rhodioloside
1. raw material are prepared：Rhodiola root, toasts, baking by rhodiola root removal aerial part and after cleaning under 40 DEG C of environment
Time is 2h；Dehydration rhodiola root is done and shreds and be milled into powder by baking after terminating, and after the sieve using 80 mesh, is sifted out
As the rhodiola root dry powder of raw material；
2. 20% ethanol water that quality is 15 times of the rhodiola root dry powder that 1. step obtains is prepared, by 20% ethanol water
PH value is allocated to 7.8；The rhodiola root dry powder that 1. step is obtained is put into 20% deployed ethanol water；Then will add
20% ethanol water of rhodiola root dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
3. 20% ethanol water that rhodiola root dry powder will be added is heated to 40 DEG C, then carries out ultrasonic wave with the power of 500W
Vibration extraction, ultrasonic activation extraction time is 50min；
4. the extract solution for 3. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, rhodioloside needed for obtaining, the rhodioloside is in light brown or white powder；
3）The extraction of total triterpene saponin
1. raw material are prepared：Pseudo-ginseng, toasts after the pseudo-ginseng is cleaned under 40 DEG C of environment, and baking time is 2h；Baking terminates
Dehydration pseudo-ginseng is done into chopping afterwards and powder is milled into, after the sieve using 60 mesh, that is sifted out is as the three of raw material
Seven dry powder；
2. the pseudo-ginseng dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing pseudo-ginseng dry powder；
3. 75% ethanol water that quality is 30 times of the degreasing pseudo-ginseng dry powder that 2. step obtains is prepared, by 75% ethanol water
PH value allocate to 7.5；The degreasing pseudo-ginseng dry powder that 2. step is obtained is put into 75% deployed ethanol water；Then will
75% ethanol water for adding degreasing pseudo-ginseng dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is heated to 40 DEG C, then carries out ultrasound with the power of 800W
Ripple vibration extraction, ultrasonic activation extraction time is 8min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, total triterpene saponin needed for obtaining, the total triterpene saponin is in light brown or white powder；
4）The extraction of naringin
1. raw material are prepared：Grapefruit peel, toasts after the grapefruit peel is cleaned under 40 DEG C of environment, and baking time is 2h；
Dewatered grape shaddock ped is done and shreds and be milled into powder by baking after terminating, and after the sieve using 60 mesh, being for being sifted out is made
It is the grapefruit peel dry powder of raw material；
2. the grapefruit peel dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to be carried out at degreasing for degreasing agent
Air-dried after reason, that is, obtain degreasing grapefruit peel dry powder；
3. 90% ethanol water that quality is 30 times of the degreasing grapefruit peel dry powder that 2. step obtains is prepared, by 90% ethanol water
The pH value of solution is allocated to 9；The degreasing grapefruit peel dry powder that 2. step is obtained is put into 90% deployed ethanol water；
Then 90% ethanol water that degreasing grapefruit peel dry powder will be added is positioned over the ultrasonic activation machine for being provided with firing equipment
It is interior；
4. 90% ethanol water that degreasing grapefruit peel dry powder will be added is heated to 85 DEG C, is then carried out with the power of 500W
Ultrasonic activation is extracted, and ultrasonic activation extraction time is 1.5h；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, naringin needed for obtaining, the naringin is in faint yellow or off-white powder shape；
5）The extraction of Paeoniflorin
1. raw material are prepared：Peony Root, toasts after the Peony Root is cleaned under 40 DEG C of environment, and baking time is 2h；Baking
Dehydration Peony Root is done after end is shredded and is milled into powder, after the sieve using 60 mesh, that is sifted out is as former material
The Peony Root dry powder of material；
2. the Peony Root dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing Peony Root dry powder；
3. it is taking off that 2. step obtains to prepare the cellulase and quality that quality is the degreasing Peony Root dry powder 1% that 2. step obtains
The pure water of 8 times of fat Peony Root dry powder；Degreasing Peony Root dry powder, cellulase, pure water are mixed and stirred for uniformly；
4. the mixed liquor that 3. step obtains is heated to 30 DEG C, soaking time 3h；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and macroporous absorbent resin is then put into surplus solution
Post, keeps 10min；
6. the large pore resin absorption column for having adsorbed extract that 5. step obtains is cleaned using pure water, then again using anhydrous
Ethanol elution, obtains eluent；
7. the eluent that 6. step obtains is placed under closed environment and spontaneously dried, that is, Paeoniflorin needed for obtaining, the Paeoniflorin
In sepia or off-white powder shape；
6）Target glucoside extract is produced
By 1）~5）The various glucoside extracts for obtaining uniformly are mixed by following weight portion：25 parts of Charantin, 12 parts of rhodioloside,
5 parts of total triterpene saponin, 15 parts of naringin, 8 parts of Paeoniflorin, that is, it is hypoglycemic needed for obtaining to use glucoside extract.
Embodiment 2：
A kind of hypoglycemic composition for using glucoside extract, following weight portion is constituted：32 parts of Charantin, 15 parts of rhodioloside, total three
8 parts of terpene saponin, 20 parts of naringin, 10 parts of Paeoniflorin.
The manufacture method of above-mentioned hypoglycemic use glucoside extract, comprises the following steps：
1）The extraction of Charantin
1. raw material are prepared：Balsam pear, toasts after removing the peel the balsam pear, remove flesh, remove seed under 60 DEG C of environment, and baking time is
3h；Dewatered bitter gourd is done and shreds and be milled into powder by baking after terminating, and after the sieve using 100 mesh, being for being sifted out is made
It is the bitter melon powder of raw material；
2. the bitter melon powder that 1. step obtains is placed in Soxhlet extractor and is used after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing bitter melon powder；
3. the degreasing bitter melon powder for 2. step being obtained is put into the absolute ethyl alcohol of 60 times of its quality multiple；Then will add de-
The absolute ethyl alcohol of fat bitter melon powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the absolute ethyl alcohol that degreasing bitter melon powder will be added is heated to 70 DEG C, then carries out ultrasonic activation with the power of 450W
Extract, ultrasonic activation extraction time is 55min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Charantin needed for obtaining, the Charantin is in brownish-yellow powder shape；
2）The extraction of rhodioloside
1. raw material are prepared：Rhodiola root, toasts, baking by rhodiola root removal aerial part and after cleaning under 60 DEG C of environment
Time is 3h；Dehydration rhodiola root is done and shreds and be milled into powder by baking after terminating, and after the sieve using 100 mesh, is sifted out
Be as raw material rhodiola root dry powder；
2. 20% ethanol water that quality is 20 times of the rhodiola root dry powder that 1. step obtains is prepared, by 20% ethanol water
PH value is allocated to 8.5；The rhodiola root dry powder that 1. step is obtained is put into 20% deployed ethanol water；Then will add
20% ethanol water of rhodiola root dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
3. 20% ethanol water that rhodiola root dry powder will be added is heated to 60 DEG C, then carries out ultrasonic wave with the power of 600W
Vibration extraction, ultrasonic activation extraction time is 60min；
4. the extract solution for 3. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, rhodioloside needed for obtaining, the rhodioloside is in light brown or white powder；
3）The extraction of total triterpene saponin
1. raw material are prepared：Pseudo-ginseng, toasts after the pseudo-ginseng is cleaned under 60 DEG C of environment, and baking time is 3h；Baking terminates
Dehydration pseudo-ginseng is done into chopping afterwards and powder is milled into, after the sieve using 80 mesh, that is sifted out is as the three of raw material
Seven dry powder；
2. the pseudo-ginseng dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing pseudo-ginseng dry powder；
3. 75% ethanol water that quality is 40 times of the degreasing pseudo-ginseng dry powder that 2. step obtains is prepared, by 75% ethanol water
PH value allocate to 8；The degreasing pseudo-ginseng dry powder that 2. step is obtained is put into 75% deployed ethanol water；Then will add
75% ethanol water for having entered degreasing pseudo-ginseng dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is heated to 60 DEG C, then carries out ultrasound with the power of 1000W
Ripple vibration extraction, ultrasonic activation extraction time is 15min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, total triterpene saponin needed for obtaining, the total triterpene saponin is in light brown or white powder；
4）The extraction of naringin
1. raw material are prepared：Grapefruit peel, toasts after the grapefruit peel is cleaned under 60 DEG C of environment, and baking time is 3h；
Dewatered grape shaddock ped is done and shreds and be milled into powder by baking after terminating, and after the sieve using 80 mesh, being for being sifted out is made
It is the grapefruit peel dry powder of raw material；
2. the grapefruit peel dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to be carried out at degreasing for degreasing agent
Air-dried after reason, that is, obtain degreasing grapefruit peel dry powder；
3. 90% ethanol water that quality is 40 times of the degreasing grapefruit peel dry powder that 2. step obtains is prepared, by 90% ethanol water
The pH value of solution is allocated to 10；The degreasing grapefruit peel dry powder that 2. step is obtained is put into 90% deployed ethanol water；
Then 90% ethanol water that degreasing grapefruit peel dry powder will be added is positioned over the ultrasonic activation machine for being provided with firing equipment
It is interior；
4. 90% ethanol water that degreasing grapefruit peel dry powder will be added is heated to 90 DEG C, is then carried out with the power of 600W
Ultrasonic activation is extracted, and ultrasonic activation extraction time is 2h；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, naringin needed for obtaining, the naringin is in faint yellow or off-white powder shape；
5）The extraction of Paeoniflorin
1. raw material are prepared：Peony Root, toasts after the Peony Root is cleaned under 60 DEG C of environment, and baking time is 3h；Baking
Dehydration Peony Root is done after end is shredded and is milled into powder, after the sieve using 80 mesh, that is sifted out is as former material
The Peony Root dry powder of material；
2. the Peony Root dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing Peony Root dry powder；
3. it is taking off that 2. step obtains to prepare the cellulase and quality that quality is the degreasing Peony Root dry powder 3% that 2. step obtains
The pure water of 10 times of fat Peony Root dry powder；Degreasing Peony Root dry powder, cellulase, pure water are mixed and stirred for uniformly；
4. the mixed liquor that 3. step obtains is heated to 50 DEG C, soaking time 4h；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and macroporous absorbent resin is then put into surplus solution
Post, keeps 15min；
6. the large pore resin absorption column for having adsorbed extract that 5. step obtains is cleaned using pure water, then again using anhydrous
Ethanol elution, obtains eluent；
7. the eluent that 6. step obtains is placed under closed environment and spontaneously dried, that is, Paeoniflorin needed for obtaining, the Paeoniflorin
In sepia or off-white powder shape；
6）Target glucoside extract is produced
By 1）~5）The various glucoside extracts for obtaining uniformly are mixed by following weight portion：32 parts of Charantin, 15 parts of rhodioloside,
8 parts of total triterpene saponin, 20 parts of naringin, 10 parts of Paeoniflorin, that is, it is hypoglycemic needed for obtaining to use glucoside extract.
Present invention is particularly suitable for severe diabetic or the diabetic of complication is produced, due to that will not produce
The resistance to the action of a drug or other side effects, the hypoglycemic suitable long-term taking of glucoside extract of the invention are taken good care of.
The foregoing description of the disclosed embodiments, only for enabling professional and technical personnel in the field to realize or using this
Invention.Various modifications to these embodiments will be apparent for those skilled in the art, institute herein
The General Principle of definition can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent scope most wide of point.
Claims (2)
1. a kind of manufacture method of hypoglycemic use glucoside extract, it is characterised in that comprise the following steps：
1）The extraction of Charantin
1. raw material are prepared：Balsam pear, toasts, during baking after removing the peel the balsam pear, remove flesh, remove seed under 40 DEG C of -60 DEG C of environment
Between be 2h-3h；Dewatered bitter gourd is done and shreds and be milled into powder by baking after terminating, and after the sieve using 40-100 mesh, is sieved
What is gone out is as the bitter melon powder of raw material；
2. the bitter melon powder that 1. step obtains is placed in Soxhlet extractor and is used after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing bitter melon powder；
3. the degreasing bitter melon powder for 2. step being obtained is put into the absolute ethyl alcohol of its 50-60 times of quality multiple；Then will add
The absolute ethyl alcohol of degreasing bitter melon powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the absolute ethyl alcohol that degreasing bitter melon powder will be added is heated to 60 DEG C -70 DEG C, is then carried out with the power of 350W-450W
Ultrasonic activation is extracted, and ultrasonic activation extraction time is 35min-55min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Charantin needed for obtaining, the Charantin is in brownish-yellow powder shape；
2）The extraction of rhodioloside
1. raw material are prepared：Rhodiola root, dries by rhodiola root removal aerial part and after cleaning under 40 DEG C of -60 DEG C of environment
Roasting, baking time is 2h-3h；Dehydration rhodiola root is done and shreds and be milled into powder by baking after terminating, using the sieve of 80-100 mesh
After sieving, that is sifted out is as the rhodiola root dry powder of raw material；
2. 20% ethanol water that quality is 15-20 times of the rhodiola root dry powder that 1. step obtains is prepared, 20% ethanol is water-soluble
The pH value of liquid is allocated to 7.8-8.5；The rhodiola root dry powder that 1. step is obtained is put into 20% deployed ethanol water；So
20% ethanol water that rhodiola root dry powder will be added afterwards is positioned in the ultrasonic activation machine for being provided with firing equipment；
3. 20% ethanol water that rhodiola root dry powder will be added is heated to 40 DEG C -60 DEG C, then with the power of 500W-600W
Ultrasonic activation extraction is carried out, ultrasonic activation extraction time is 50min-60min；
4. the extract solution for 3. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, rhodioloside needed for obtaining, the rhodioloside is in light brown or white powder；
3）The extraction of total triterpene saponin
1. raw material are prepared：Pseudo-ginseng, toasts after the pseudo-ginseng is cleaned under 40 DEG C of -60 DEG C of environment, and baking time is 2h-3h；
Dehydration pseudo-ginseng is done and shreds and be milled into powder by baking after terminating, and after the sieve using 60-80 mesh, that is sifted out is conduct
The pseudo-ginseng dry powder of raw material；
2. the pseudo-ginseng dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing pseudo-ginseng dry powder；
3. 75% ethanol water that quality is 30-40 times of the degreasing pseudo-ginseng dry powder that 2. step obtains is prepared, by 75% ethanol water
The pH value of solution is allocated to 7.5-8；The degreasing pseudo-ginseng dry powder that 2. step is obtained is put into 75% deployed ethanol water；
Then 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 75% ethanol water that degreasing pseudo-ginseng dry powder will be added is heated to 40 DEG C -60 DEG C, then with the work(of 800W-1000W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 8min-15min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, total triterpene saponin needed for obtaining, the total triterpene saponin is in light brown or white powder；
4）The extraction of naringin
1. raw material are prepared：Grapefruit peel, toasts, baking time after the grapefruit peel is cleaned under 40 DEG C of -60 DEG C of environment
It is 2h-3h；Dewatered grape shaddock ped is done and shreds and be milled into powder by baking after terminating, and after the sieve using 60-80 mesh, is sieved
What is gone out is as the grapefruit peel dry powder of raw material；
2. the grapefruit peel dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to be carried out at degreasing for degreasing agent
Air-dried after reason, that is, obtain degreasing grapefruit peel dry powder；
3. 90% ethanol water that quality is 30-40 times of the degreasing grapefruit peel dry powder that 2. step obtains is prepared, by 90% second
The pH value of alcohol solution is allocated to 9-10；The degreasing grapefruit peel dry powder that 2. step is obtained is put into 90% deployed ethanol water
In solution；Then 90% ethanol water that degreasing grapefruit peel dry powder will be added is positioned over the ultrasound for being provided with firing equipment
In ripple bobbing machine；
4. 90% ethanol water that degreasing grapefruit peel dry powder will be added is heated to 85 DEG C -90 DEG C, then with 500W-600W
Power carry out ultrasonic activation extraction, ultrasonic activation extraction time is 1.5h-2h；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then does surplus solution naturally under closed environment
Dry, that is, naringin needed for obtaining, the naringin is in faint yellow or off-white powder shape；
5）The extraction of Paeoniflorin
1. raw material are prepared：Peony Root, toasts after the Peony Root is cleaned under 40 DEG C of -60 DEG C of environment, and baking time is 2h-
3h；Baking the dry chopping of dehydration Peony Root and will be milled into powder after terminating, after the sieve using 60-80 mesh, sifted out i.e.
It is as the Peony Root dry powder of raw material；
2. the Peony Root dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing Peony Root dry powder；
3. it is what 2. step obtained to prepare the cellulase and quality that quality is the degreasing Peony Root dry powder 1%-3% that 2. step obtains
The pure water of 8-10 times of degreasing Peony Root dry powder；Degreasing Peony Root dry powder, cellulase, pure water are mixed and stirred for uniformly；
4. the mixed liquor that 3. step obtains is heated to 30 DEG C -50 DEG C, soaking time 3h-4h；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and macroporous absorbent resin is then put into surplus solution
Post, keeps 10min-15min；
6. the large pore resin absorption column for having adsorbed extract that 5. step obtains is cleaned using pure water, then again using anhydrous
Ethanol elution, obtains eluent；
7. the eluent that 6. step obtains is placed under closed environment and spontaneously dried, that is, Paeoniflorin needed for obtaining, the Paeoniflorin
In sepia or off-white powder shape；
6）Target glucoside extract is produced
By 1）~5）The various glucoside extracts for obtaining uniformly are mixed by following weight portion：Charantin 25-32 parts, rhodioloside 12-
15 parts, 5-8 parts of total triterpene saponin, naringin 15-20 parts, Paeoniflorin 8-10 parts, that is, hypoglycemic use glucoside extract needed for obtaining.
2. the hypoglycemic of manufacture method manufacture uses glucoside extract according to claim 1, it is characterised in that：The hypoglycemic use
Glucoside extract is made up of the composition of following weight portion：Charantin 25-32 parts, rhodioloside 12-15 parts, total triterpene saponin 5-8
Part, naringin 15-20 parts, Paeoniflorin 8-10 parts.
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