CN1297353A

CN1297353A - Hypoylycemic agents

Info

Publication number: CN1297353A
Application number: CN99805141A
Authority: CN
Inventors: 纪再思; 伊藤裕之; 王晓林; 陶建生
Original assignee: SHANGHAI MEIJI HEALTH PHARMACEUTICAL CORP Ltd
Current assignee: SHANGHAI MEIJI HEALTH PHARMACEUTICAL CORP Ltd
Priority date: 1998-04-17
Filing date: 1999-04-16
Publication date: 2001-05-30
Also published as: KR20010074477A; KR100601191B1; AU3344499A; WO1999053936A1

Abstract

Hypoglycemic agents containing the active ingredient corn female inflorescence extract; and drinks and foods containing the same.

Description

Hypoylycemic agents

Technical scope

The present invention relates to the corn silk extract as the Hypoylycemic agents of active ingredient and contain the diet product of this extract.

Background technology

In recent years, the sickness rate of diabetes has the tendency that increases together with hypertension, cancer.This surge that increases the old-aged diabetic of the carbohydrate tolerance that causes due to descending with abundant calorie intake surplus, work hypomotility or the stress etc. that bring of dietetic life, age is relevant.

Diabetes are the disease that chronic generalized metabolic disorder causes, show as chronic hyperglycemia state.It is relevant with the multiple factor such as unusual of various environmental factors, insulin receptor gene or sugared transport agent gene that its morbidity is considered to.The main regulatory factors of blood glucose is that pancreas B cell is synthetic, excretory insulin, and hyperglycemia lacks insulin or its effect antagonism factor surplus causes.The shortage of insulin causes the Developmental and Metabolic Disorder of sugar, protein, fat, and the result causes various complication such as acidosis, kidney and amphiblestroid blood capillary are unusual, peripheral nerve obstacle, arteriosclerosis development promotion.

The target for the treatment of diabetes for remove whole body institute in a organized way insulin action deficiency, make metabolism normalization, and make it to keep, to prevent, to suppress these acute and chronic complication.

The treatment of diabetes and carbohydrate tolerance obstacle generally has food therapy (control heat) and exercise therapy, well in the blood sugar control value, is using pharmacotherapy with these Therapeutic Method.Dietetic therapy or exercise therapy become the huge sensual pressure that reaches mentally for the patient.In addition, the oral Hypoylycemic agents that requires pharmacotherapy to use is the medicine that has no side effect, can continue medication for a long time, use safely.

In this case, seek the composition that has hypoglycemic activity in the edible usually food of people.Various food components have been found that with hypoglycemic activity.For example, found that the hemicellulose part analyte of corn epidermis gained is as effective ingredient carbohydrate tolerance disorder improving agents.(spy opens flat 7-324036 number).Yet previous invention does not find that the corn silk extract has the blood sugar lowering effect.

So the present invention is to provide the novel extracts from crude drugs with good hypoglycemic activity as problem.

Disclosure of an invention

Present inventors further investigate in order to solve above-mentioned problem, find that the corn silk extract has good hypoglycemic activity, thereby have finished the present invention.

Promptly the invention provides with the Hypoylycemic agents of corn silk extract as effective ingredient.

In addition, the present invention also provides the diet product with blood sugar lowering or inhibition blood glucose value effect of increasing that contain the corn silk extract.

Further, the invention provides the corn silk extract in the drink Application in Food that has blood sugar lowering value or inhibition blood glucose value effect of increasing for manufacturing.

And the invention provides Therapeutic Method, it is characterized in that giving the corn silk extract diabetics.

Simple declaration Fig. 1 of accompanying drawing represents the gel permeation chromatography of corn silk extract.Post: Superdex 30 prep grade column lengths: 16mm ID* 60cmL flow velocity: 1.0mL/min sample size: 50 μ L eluents: 10mM HEPES-NaOH (pH7.4)/1M NaCl Fig. 2 is for to bring out rats with diabetes for ST2, the blood glucose reduction effect of corn silk extract performance.

The optimised form that carries out an invention

Corn silk is the style and stigma (the Corn silk of the new flower of grass family (Gramineae) corn Zea mays L.; Be called corn silk).Its place of production is distributed widely in China, Japan, Korea S etc.As pharmacological action, and effects such as the diuresis of existing report, blood pressure lowering, the hidropoiesis of promotion gallbladder (difficult ripple is permanent male: " oriental drug encyclopaedia illustrated handbook " [II]: 131, put down into (strain) child care society 6 years).

The used corn silk of the present invention can be to derive from the corn of all kinds of cultivation all over the world, but the kind of preferably using Chinese Shandong Province to produce is the dry product of tucking in list No. 12 or tucking in single No. 14 corn silk.

The extract of corn silk can adopt the general extracting method of vegetable crude drug active ingredient to obtain.

For example, take the corn silk after drying.Use solvent extraction then.Use about 10-30 times of water, aqueous ethanol or the weakly acidic water etc. that chop up or pulverize the back test portion during extraction.Extracting temperature is the boiling temperature of room temperature～extraction solvent, preferably extracts in hot water.Extraction time, carry out 30 minutes～a few hours for stirring down when using hot water.The gained extracting solution can directly use as corn silk extract of the present invention; Be the further effective ingredient of concentrated extracting solution, can be with extracting liquid filtering, centrifugalize, solid-liquid phase separately, liquid phase is partly reduced pressure (below 95 ℃) concentrate.Concentrated solution re-uses spray drying method, hypobaric drying method or freezing and drying lyophilization and carries out drying, also can be made into powder.In addition, as required, the also available chromatography of this extracting solution is carried out further refining.

So the corn silk extract of gained just has hypoglycemic activity separately, uses but extract composition with other food with hypoglycemic activity, is expected to obtain the effect of collaborative or adduction.For example, the mycelial hot water extract of cultivation of Cordyceps (Cordyceps sinensis (Berkeley) Saccard) has various pharmacological actions such as heart tonifying, blood pressure lowering, antitussive, resisting fatigue and hypoglycemic activity, this extract and corn silk use as pharmaceuticals together, perhaps make an addition to the hypoglycemic activity of estimating can obtain having collaborative or adduction in the diet product.

The corn silk extract is risen when suppressing medicine and using as blood sugar lowering or blood glucose, can add appropriate excipients (lactose, corn starch, crystalline cellulose etc.), be mixed and made into various dosage form (tablet, capsule, powder, granula subtilis, granule etc.) that can be oral.In addition, add to the extract of corn silk in the diet product on a small quantity and do not influence its local flavor, the glycosuria condition of disease in the daily food life is played improve or preventive effect.

When corn silk extract of the present invention was used for pharmaceuticals as Hypoylycemic agents or blood glucose rising inhibitor, this extract can give with the amount per os of its drying solid composition 0.1～20g/kg the adult.

Embodiment

Below just embodiment the present invention is specifically described, but the present invention is not limited thereto.

The manufacturing of embodiment 1 extract

The water logging bubble that the dry product of corn silk (tucking in single No. 12 of Chinese Shandong Province product) adds about 15 times of amounts boiled and extracted active ingredient in 1 hour after 30 minutes.Extract the water that adds about 8 times of amounts in the residue, boiled 1 hour.Merge extractive liquid, filters with 100 purposes sieve.It is 1.08 that filtrate decompression is concentrated to proportion.Concentrated solution carries out centrifugalize (2000r.p.m20 minute), and the supernatant pasteurization obtains the hot water extract of corn silk.Fig. 1 is the gel permeation chromatography figure of this extract (drying solid composition 3.33g/100ml).Embodiment 2 contains the Production Example of the diet product of extract.

In corn silk extract 65.6kg, Cordyceps hot water extract (with reference to WO96/00580) 3.33kg (solid constituent 50mg/ml), sweeting agent 5.0kg, citric acid 0.5kg, malic acid 0.5kg and the spice 0.1kg of embodiment 1 gained, add entry to 100kg, stirring and dissolving.Add the 0.07kg antiseptic that is dissolved in 0.51 ethanol again.Pack into behind the pre-sterilizing in the bottle, in 90 ℃ of hot water, soaked 2 hours.Naturally put cold 1 evening then, obtain containing the beverage product of extract of the present invention.The test of embodiment 3 blood sugar lowering

The SD in 7 ages in week is after male rat is raised a week, every rat tail vein injection streptozotocin (streptozotocin: STZ) 50mg/2ml/kg.Measured the blood glucose value of every rat behind the injection STZ on the 3rd day.STZ for gained brings out diabetes rat, and its blood glucose meansigma methods is roughly the same, is divided into extract administration group (6) and matched group (6).3 of the rats of normal group (not taking anything).

For extract administration group, from the injection STZ after 3 days, continuous 7 days per os give the corn silk extract (drying solid composition 4.07g/kg/ day) of embodiment 1 gained.Matched group gives distilled water.Each variation in the daytime of organizing blood glucose value as shown in Figure 2.From the visible following result of Fig. 2.(1) blood glucose value of normal group is about 110mg/dL at duration of test, demonstrates roughly certain fluctuation.Corresponding, it was 557mg/dL on administration same day that STZ brings out diabetic groups, compared with normal group and demonstrated the hyperglycemia value, demonstrated the hyperglycemia value that remains on about 530mg/dL later on.(2) carry out the blood sugar lowering test for bring out diabetes rat with this STZ, extract administration group was administration the 3rd day, and blood glucose value is 370mg/dL, compares with matched group to demonstrate obvious reduction.After, blood glucose value fluctuates in certain low value of 400mg/dL roughly.(3) above as seen, the extract of corn silk have hypoglycemic activity or suppress the blood glucose value effect of increasing, for the prevention of diabetes or treat significant effective.

Utilize scope on the industry

The present invention finds that the extract of corn silk has obvious hypoglycemic value effect. This extraction Thing can be used as the prevention, therapeutic agent of diabetes or as arteriosclerotic blood due to the sugar tolerance obstacle The prophylactic of pipe obstacle. In addition, this extract is added in the diet product, by the picked-up to it, Can obtain in daily life the improvement that continues or the effect of preventing diabetes.

Claims

1. with the Hypoylycemic agents of corn silk extract as active ingredient.

2. according to the Hypoylycemic agents of claim 1 record, corn silk wherein derives from the kind of producing Chinese Shandong Province and tucks in list No. 12 or tuck in single No. 14 corns.

3. what contain the corn silk extract has hypoglycemic activity or blood glucose value inhibiting diet product that rise.

4. according to the diet product of claim 3 record, wherein to derive from the kind of producing Chinese Shandong Province be to tuck in single No. 12 or tuck in singly No. 14 corn to corn silk.

5. the corn silk extract has the effect of blood sugar lowering value or the blood glucose value inhibiting drink Application in Food that rises in manufacturing.

6. according to the use of claim 5, wherein to derive from the kind of producing Chinese Shandong Province be to tuck in single No. 12 or tuck in single No. 14 corn to corn silk.

7. a treatment of diabetes method is characterized in that taking the corn silk extract.

8. according to the Therapeutic Method of claim 7 record, wherein to derive from the kind of producing Chinese Shandong Province be to tuck in single No. 12 or tuck in single No. 14 corn to corn silk.