CN107712170A - A kind of preparation method and applications of soluble dietary fiber - Google Patents
A kind of preparation method and applications of soluble dietary fiber Download PDFInfo
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- CN107712170A CN107712170A CN201710915616.5A CN201710915616A CN107712170A CN 107712170 A CN107712170 A CN 107712170A CN 201710915616 A CN201710915616 A CN 201710915616A CN 107712170 A CN107712170 A CN 107712170A
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- Prior art keywords
- dietary fiber
- soluble dietary
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- solution
- preparation
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/22—Drying or concentrating tea extract
- A23F3/225—Drying or concentrating tea extract by evaporation, e.g. drying in thin layers, foam drying
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a kind of preparation method of soluble dietary fiber, comprise the following steps:(1) tealeaves leftover bits and pieces is taken, dries, grinds, is sieved;(2) alpha amylase enzymolysis is added in tealeaves leftover bits and pieces after sizing, regulation pH value of solution is 6.0~6.8, and 4~6h is digested at a temperature of 40~50 DEG C;(3) pH to 4.2~5.0 of solution is adjusted, adds cellulase, 1~3h is digested under the conditions of 45~55 DEG C;(4) pH value of solution in step (3) is adjusted to 6.6~7.0, adds papain, digested 1~3 hour at 55~60 DEG C;(5) solution boils 5~10min, filtering, takes clear liquid to concentrate, and with the 95% ethanol alcohol precipitation 8~10 hours of 2~4 times of volumes, 4000r/min is centrifuged 10 minutes, the sediment fraction after centrifugation is evaporated near dry, re-dry, obtains soluble dietary fiber solid.The beverage prepared with obtained soluble dietary fiber remains functional component in tealeaves, and stability is high, suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of food auxiliary material and preparation method thereof and its application, more particularly to a kind of soluble dietary fiber
And its application.
Background technology
Dietary fiber refer to can not by edible plants cell that digestion is digested and assimilated, lignin, polysaccharide and
The summation of related substances, in terms of specific chemical constituent, dietary fiber includes cellulose, hemicellulose, pectin, glucan, wood
Quality etc..Dietary fiber is that health diet is indispensable, and it remains digestive system health, while it is fine to absorb enough meals
Dimension can also prevent diabetes, angiocardiopathy, cancer and other some diseases.Dietary fiber can be divided into two classes, soluble meals
Fiber (SDF) and insoluble dietary fiber (IDF) are eaten, SDF is water-soluble, from pectin, phycocolloid, konjaku etc., there is reduction
Blood fat and blood glucose etc. act on.
Tea, it is bud-leaf processed manufactured a kind of low heat value, the non-alcoholic drink of Theaceae Camellia Plants tea.Tealeaves
Composition mainly includes water, protein, amino acid, caffeine, polyatomic phenol, carbohydrate, lipid, mineral matter, organic acid etc..
Tea is as drink, and mainly edible is its water soluble ingredient.Tea has long medicinal history and wide in range health-care efficacy, early
Just on the books before thousands of years, tealeaves was once referred to as " medicines of ten thousand diseases ".As people deepen continuously to tealeaves research, the guarantor of tea
Strong effect increasingly causes the concern of all circles.
Chinese tea aboundresources, the prospect of comprehensive development and utilization are very wide.The tea products of research report are more at present
See extraction of functional components etc. in tea beverage, tea health-care food, tealeaves.Produce green tea soluble dietary fiber beverage
Research is not yet seen in report.
Traditional tea dietary fiber extraction technique uses chemical method more, but this method can pollute to environment, extract simultaneously
Also chemical substance can be introduced in thing.The present invention prepares soluble dietary fiber using the method for cellulase degradation, green,
Yield is high, has good popularizing application prospect.
The content of the invention
Goal of the invention:It is an object of the invention to provide a kind of soluble meals extracted using enzyme process from tealeaves leftover bits and pieces
The method for eating fiber.Another object of the present invention is to provide the application of the soluble dietary fiber in the beverage;In addition, this hair
The bright optimum formula for additionally providing the beverage for including the soluble dietary fiber.
Technical scheme:A kind of preparation method of soluble dietary fiber of the present invention, comprises the following steps:(1) tea is taken
Leaf leftover bits and pieces, drying, grinds, and sieves;(2) enzymolyzing alpha-amylase is added in tealeaves leftover bits and pieces after sizing, adjusts pH value of solution
For 6.0-6.8,4-6h is digested at a temperature of 40-50 DEG C;(3) in regulating step (2) solution pH to 4.2-5.0, add fiber
Plain enzyme, digests 1-3h under the conditions of 45-55 DEG C;(4) pH value of solution in step (3) is adjusted to 6.6-7.0, adds Papain
Enzyme, 1-3 hours are digested at 55-60 DEG C;(5) solution after being digested in step (4) is boiled into 5-10min, filters, take clear liquid dense
Contracting, with 95% ethanol alcohol precipitation 8-10 hours of 2-4 times of volume, is centrifuged 10 minutes under 4000r/min rotating speeds, will be heavy after centrifugation
Forming sediment, it is near dry to be partly evaporated to, and is being dried, is obtaining soluble dietary fiber and carry solid.It is to improve that precipitation, which is evaporated to closely dry,
Dry efficiency, the degree that soluble dietary fiber extract solution is evaporated to close drying are the surface of solids without visible liquid.
In step (1), the aperture of the sieve is 60 mesh or 80 mesh.
In step (2), the mass ratio of the alpha-amylase and tealeaves leftover bits and pieces is 0.04:1~0.08:1.
In step (3), the mass ratio of the cellulase and tealeaves leftover bits and pieces is 0.06:1~0.1:1.
In step (4), the mass ratio of the papain and tealeaves leftover bits and pieces is 0.06:1~0.1:1.
The soluble dietary fiber prepared by the above method.
The application of above-mentioned soluble dietary fiber in the beverage.
The beverage described above for including soluble dietary fiber, includes following components:In terms of weight/mass percentage composition, 2.8%
~3.2% soluble dietary fiber, 0.15%~0.25% compound stabilizer, 5.5%~6.5% white granulated sugar, 0.05%~
0.15% citric acid and 0.03%~0.06% vitamin C.
The compound stabilizer is 3 by mass ratio:1~2:1 sodium carboxymethylcellulose forms with xanthans.
Beneficial effect:
1st, the method that the present invention prepares soluble dietary fiber, can efficiently extract soluble dietary fiber, recovery rate
Up to more than 60%.
2nd, while the beverage for preparing of the present invention provides abundant soluble dietary fiber, remain in tealeaves function into
Point, including Tea Polyphenols etc., have to refresh oneself and recover from fatigue, reduce cholesterol, reducing blood lipid blood glucose, fall fire, protection eyesight, prevent the work(such as treat constipation
Energy.
3rd, in beverage prepared by the soluble dietary fiber prepared using the present invention, soluble dietary fibre content 2.8-
3.2%, total plate count is less than 100CFU/mL.This drink color and luster is in light yellow, and state stable homogeneous, taste is sour-sweet, there is green tea wind
Taste.
Brief description of the drawings
Fig. 1 is the process chart of the present invention.
Embodiment
First, raw material sources
1st, tealeaves leftover bits and pieces derives from Jiangsu Cha Boyuan Ming Yuan companies, and tealeaves leftover bits and pieces of the present invention is not limited in addition
The kind of the tealeaves such as black tea or green tea.
2nd, alpha-amylase derives from Henan An Rui bio tech ltd.
3rd, cellulase derives from Henan Yang Shao bioengineering Co., Ltd.
4th, papain derives from Henan An Rui bio tech ltd.
5th, remaining material is gained purchased in market.
2nd, detection method
1st, the assay method of soluble dietary fiber recovery rate
95% ethanol alcohol precipitation 8~10 hours by dietary fiber extract solution with 4 times of volumes, 4000r/min are centrifuged 10 minutes
Taking precipitate is dried afterwards, weighs precipitation quality, as SDF mass.Soluble Fiber recovery rate calculation formula is as follows:
2nd, total plate count in beverage
Meet《GB7101-2015》Standard provides, and the bacterium colony detected with reference to the detection method wherein provided in beverage is total
Number.
3rd, the assay method of beverage stability
The beverage 10mL prepared is accurately added in centrifuge tube, then 15min is centrifuged in 4000r/min, discards top
Solution, Sediment weight is accurately weighed, precipitation capacity is calculated using formal.
3rd, sample preparation
The preparation of 3.1 soluble dietary fibers
Embodiment 1:200mL citric acid of the tealeaves leftover bits and pieces addition containing 0.8g alpha-amylases after 10g is crushed is weighed to delay
Fliud flushing (pH 6.4), digested 4 hours in 45 DEG C of water-baths.Enzymolysis liquid pH to 4.8 is adjusted, adds 1.0g cellulases at 50 DEG C
Enzymolysis 3 hours.Enzymolysis liquid pH to 6.8 is adjusted, 0.6g papains is added and is digested 3 hours at 60 DEG C.By enzyme after having digested
Solution liquid boils 10 minutes, filters, takes clear liquid to concentrate, and with the 95% ethanol alcohol precipitation 9 hours of 3 times of volumes, 4000r/min centrifuges 10 points
Clock.Sediment fraction after centrifugation is evaporated to and closely done, re-dry, obtains soluble dietary fiber solid.Take 0.4g compound stabilizers
(sodium carboxymethylcellulose and xanthans mass ratio 3:1) it is well mixed with 12g white granulated sugars, adds solubility prepared by above step
Dietary fiber solid 6g, 0.2g citric acids, 0.1g vitamin Cs are added, stirring, adds water to be settled to 200mL, is well mixed, will drink
Material is filled into the vial after washing and sterilizing, is sterilized 20 minutes at 95 DEG C, and finished product is made in sealing.
Embodiment 2:Weigh the tealeaves leftover bits and pieces after 100g is crushed, 7g alpha-amylases are dissolved in 2000mL citrate buffer solutions
(pH 6.4), digested 5 hours in 48 DEG C of water-baths.Enzymolysis liquid pH to 4.8 is adjusted, 10g cellulases is added and digests 3 at 50 DEG C
Hour.Enzymolysis liquid pH to 7.0 is adjusted, 7g papains is added and is digested 2.5 hours at 56 DEG C.Enzymolysis liquid is boiled after having digested
Boiling 10 minutes, filtering, takes clear liquid to concentrate, and with the 95% ethanol alcohol precipitation 8 hours of 2 times of volumes, 4000r/min is centrifuged 10 minutes.Will
Sediment fraction after centrifugation, which is evaporated to, closely to be done, and re-dry, obtains soluble dietary fiber solid.Take 5g compound stabilizer (carboxymethyls
Sodium cellulosate and xanthans mass ratio 2:1) it is well mixed with 100g white granulated sugars, adds soluble dietary fiber solid 64g, is added
3g citric acids, 1.2g vitamin Cs, stirring, add water to be settled to 2000mL, are well mixed, after beverage filling is entered into washing and sterilizing
Vial, sterilize 20 minutes at 95 DEG C, finished product is made in sealing.
Embodiment 3:Weigh the tealeaves leftover bits and pieces after 100g is crushed, 5g alpha-amylases are dissolved in 2000mL citrate buffer solutions
(pH 6.6), digested 5 hours in 48 DEG C of water-baths.Enzymolysis liquid pH to 4.4 is adjusted, 6g cellulases is added and digests 3 at 50 DEG C
Hour.Enzymolysis liquid pH to 7.0 is adjusted, 9g papains is added and is digested 2.5 hours at 56 DEG C.Enzymolysis liquid is boiled after having digested
Boiling 10 minutes, filtering, takes clear liquid to concentrate, and with the 95% ethanol alcohol precipitation 8 hours of 4 times of volumes, 4000r/min is centrifuged 10 minutes.Will
Sediment fraction after centrifugation, which is evaporated to, closely to be done, and re-dry, obtains soluble dietary fiber solid.Take 2g compound stabilizer (carboxymethyls
Sodium cellulosate and xanthans mass ratio 2:1) it is well mixed with 50g white granulated sugars, adds soluble dietary fiber solid 30g, is added
1g citric acids, 0.4g vitamin Cs, stirring, add water to be settled to 1000mL, are well mixed, after beverage filling is entered into washing and sterilizing
Vial, sterilize 20 minutes at 95 DEG C, finished product is made in sealing.
4th, result determines
1st, the assay method of soluble dietary fiber recovery rate
95% ethanol alcohol precipitation 8~10 hours by dietary fiber extract solution with 4 times of volumes, 4000r/min are centrifuged 10 minutes
Taking precipitate is dried afterwards, weighs precipitation quality, as SDF mass.Soluble Fiber recovery rate calculation formula is as follows:
The Soluble Fiber recovery rate obtained in embodiment 1,2,3 is as shown in table 1.
The soluble dietary fiber recovery rate of table 1
Embodiment | Soluble dietary fiber recovery rate (%) |
Embodiment 1 | 62 |
Embodiment 2 | 65 |
Embodiment 3 | 63 |
2nd, the assay method of beverage stability
The beverage 10mL prepared is accurately added in centrifuge tube, then 15min is centrifuged in 4000r/min, discards top
Solution, Sediment weight is accurately weighed, precipitation capacity is calculated using formal.
By taking embodiment 1,2,3 as an example, beverage stability is determined, is shown in Table 2.
The beverage stability of table 2 determines
Embodiment | Precipitation capacity (%) |
Embodiment 1 | 0.212 |
Embodiment 2 | 0.230 |
Embodiment 3 | 0.226 |
The content of soluble dietary fiber in the beverage is improved in the present invention it can be seen from the result of table 2, and is prepared
Beverage systems have good stability.
Claims (9)
1. a kind of preparation method of soluble dietary fiber, it is characterised in that comprise the following steps:
(1) tealeaves leftover bits and pieces is taken, dries, grinds, is sieved;
(2) enzymolyzing alpha-amylase is added in tealeaves leftover bits and pieces after sizing, regulation pH value of solution is 6.0~6.8, at 40~50 DEG C
At a temperature of digest 4~6h;
(3) in regulating step (2) solution pH to 4.2~5.0, add cellulase, under the conditions of 45~55 DEG C digest 1~
3h;
(4) pH value of solution in step (3) is adjusted to 6.6~7.0, adds papain, it is small that 1~3 is digested at 55~60 DEG C
When;
(5) solution after being digested in step (4) is boiled into 5~10min, filters, take clear liquid to concentrate, with the 95% of 2~4 times of volumes
Ethanol alcohol precipitation 8~10 hours, 4000r/min are centrifuged 10 minutes, and the sediment fraction after centrifugation is evaporated to and closely done, re-dry, is obtained
Soluble dietary fiber solid.
2. the preparation method of soluble dietary fiber according to claim 1, it is characterised in that in step (1), the sieve
Aperture be 60 or 80 mesh.
3. the preparation method of soluble dietary fiber according to claim 1, it is characterised in that in step (2), the α-
The mass ratio of amylase and tealeaves leftover bits and pieces is 0.04:1~0.08:1.
4. the preparation method of soluble dietary fiber according to claim 1, it is characterised in that in step (3), the fibre
The mass ratio for tieing up plain enzyme and tealeaves leftover bits and pieces is 0.06:1~0.1:1.
5. the preparation method of soluble dietary fiber according to claim 1, it is characterised in that in step (4), the wood
The mass ratio of melon protease and tealeaves leftover bits and pieces is 0.06:1~0.1:1.
A kind of 6. soluble dietary fiber prepared by claim 1-5 methods.
A kind of 7. application of soluble dietary fiber as described in claim 6 in the beverage.
8. the beverage according to claim 7 for including soluble dietary fiber, it is characterised in that include following components:With
Weight/mass percentage composition meter, 2.8%~3.2% soluble dietary fiber, 0.15%~0.25% compound stabilizer, 5.5%~
6.5% white granulated sugar, 0.05%~0.15% citric acid and 0.03%~0.06% vitamin C.
9. the beverage according to claim 8 for including soluble dietary fiber, it is characterised in that the compound stabilizer by
Mass ratio is 3:1~2:1 sodium carboxymethylcellulose forms with xanthans.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110699404A (en) * | 2019-11-17 | 2020-01-17 | 横县振茂茶厂 | Method for producing dietary fiber by taking tea milli as raw material |
CN114158740A (en) * | 2021-11-05 | 2022-03-11 | 长春中医药大学 | Preparation method of hazelnut meal soluble dietary fiber and application of hazelnut meal soluble dietary fiber in regulating blood fat |
CN114586988A (en) * | 2022-03-29 | 2022-06-07 | 茅台学院 | Extraction process and application of soluble dietary fiber of holboellia latifolia peel with blood sugar reducing function |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110699404A (en) * | 2019-11-17 | 2020-01-17 | 横县振茂茶厂 | Method for producing dietary fiber by taking tea milli as raw material |
CN114158740A (en) * | 2021-11-05 | 2022-03-11 | 长春中医药大学 | Preparation method of hazelnut meal soluble dietary fiber and application of hazelnut meal soluble dietary fiber in regulating blood fat |
CN114586988A (en) * | 2022-03-29 | 2022-06-07 | 茅台学院 | Extraction process and application of soluble dietary fiber of holboellia latifolia peel with blood sugar reducing function |
CN114586988B (en) * | 2022-03-29 | 2023-10-31 | 茅台学院 | Extraction process and application of soluble dietary fiber with blood glucose reducing function for fruit skin of holboellia latifolia |
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Application publication date: 20180223 |