CN109846923B - Extraction method of Yunnan caulis spatholobi - Google Patents
Extraction method of Yunnan caulis spatholobi Download PDFInfo
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- CN109846923B CN109846923B CN201910060938.5A CN201910060938A CN109846923B CN 109846923 B CN109846923 B CN 109846923B CN 201910060938 A CN201910060938 A CN 201910060938A CN 109846923 B CN109846923 B CN 109846923B
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Abstract
The invention discloses a method for extracting Yunnan caulis spatholobi, which comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, inactivating, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating into paste. The invention has the advantages that: can effectively increase the yield of the extract and the content of effective components.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a method for extracting Yunnan caulis spatholobi.
Background
The caulis spatholobi is a traditional Chinese medicine for promoting blood circulation and removing blood stasis, is bitter, sweet and warm in nature, enters liver and kidney channels, has the effects of enriching blood and promoting blood circulation, and relaxing and activating channels and collaterals, and is used for treating diseases such as irregular menstruation, blood deficiency and sallow complexion, numbness and paralysis, rheumatic arthralgia and the like.
Caulis Spatholobi is mainly derived from stems of Schisandra Inodorata and Schisandra heteroclita of Schisandra of Schisandraceae. Yunnan caulis Spatholobi is the main ingredient of compound caulis Spatholobi extract, and when in use, Yunnan caulis Spatholobi is directly decocted with water and concentrated into extract for use. In the process of extracting Yunnan caulis Spatholobi, the extraction yield is low, and further improvement is needed.
Disclosure of Invention
The invention aims to provide an extraction method of Yunnan caulis spatholobi with high extraction yield.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
a method for extracting Yunnan caulis Spatholobi comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, inactivating, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating into paste.
Preferably, the amount of the added water is 4-8 times of the weight of the caulis spatholobi raw medicinal material; the soaking times are 1-5 times, and the soaking time is 1-5 hours each time.
Preferably, the complex enzyme consists of cellulase and alpha-amylase, and the mass ratio of the cellulase to the alpha-amylase is 2-4: 1.
Preferably, the dosage of the compound enzyme is 0.6-1% of the weight of the raw medicinal materials of the Yunnan caulis spatholobi.
Preferably, the temperature of the enzymolysis is 48-52 ℃, the pH value of the enzymolysis is 5.8-6.2, and the time of the enzymolysis is 1-3 hours.
The invention has the following technical advantages:
compared with the traditional water decoction mode, the invention adopts the compound enzyme for extraction, can effectively increase the yield of the extract and can also increase the content of effective components.
Detailed Description
The technical solutions in the present invention are described clearly and completely below, and it is obvious that the described embodiments are only some of the embodiments of the present invention, not all of them. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for extracting Yunnan caulis Spatholobi comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, heating to 100 deg.C for inactivating for 8 min, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating into paste.
Wherein the amount of the added water is 6 times of the weight of the raw medicinal materials of the Yunnan caulis spatholobi; the soaking times are 3 times, and the soaking time is 3 hours each time.
The complex enzyme consists of cellulase (10000U/g) and alpha-amylase (20000U/g), and the mass ratio of the cellulase to the alpha-amylase is 3: 1.
The dosage of the compound enzyme is 0.8 percent of the weight of the raw medicinal materials of the Yunnan caulis spatholobi.
The temperature of enzymolysis is 50 ℃, the pH value of enzymolysis is 6, and the enzymolysis time is 2 hours.
Example 2
A method for extracting Yunnan caulis Spatholobi comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, heating to 100 deg.C for inactivating for 10 min, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating into paste.
Wherein the amount of the added water is 8 times of the weight of the raw medicinal materials of the Yunnan caulis spatholobi; the soaking times are 5 times, and the soaking time is 5 hours each time.
The complex enzyme consists of cellulase (10000U/g) and alpha-amylase (20000U/g), and the mass ratio of the cellulase to the alpha-amylase is 4: 1.
The dosage of the compound enzyme is 1 percent of the weight of the raw medicinal materials of the Yunnan suberect spatholobus stem.
The temperature of enzymolysis is 52 ℃, the pH of enzymolysis is 6.2, and the enzymolysis time is 3 hours.
Example 3
A method for extracting Yunnan caulis Spatholobi comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, heating to 100 deg.C for inactivating for 5 min, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating to obtain paste.
Wherein the amount of the added water is 4 times of the weight of the raw medicinal materials of the Yunnan caulis spatholobi; the soaking times are 2 times, and the soaking time is 1 hour.
The complex enzyme consists of cellulase (10000U/g) and alpha-amylase (20000U/g), and the mass ratio of the cellulase to the alpha-amylase is 2: 1.
The dosage of the compound enzyme is 0.6 percent of the weight of the raw medicinal materials of the Yunnan caulis spatholobi.
The temperature of enzymolysis is 48 ℃, the pH value of enzymolysis is 5.8, and the enzymolysis time is 1 hour.
Comparative example 1
Directly decocting in water and concentrating to obtain paste.
Comparative example 2
Enzymatic hydrolysis was carried out using only cellulase, and the rest was carried out completely in the same manner as in example 1.
Comparative example 3
The enzymatic hydrolysis was carried out using only alpha-amylase, the rest being carried out exactly as in example 1.
In order to verify the technical effect of the invention, the following tests are specifically made and are detailed in table 1.
TABLE 1 comparison of extraction yield and effective component of Yunnan caulis Spatholobi
| Item | Yield of Yunnan caulis Spatholobi ointment (%) | Content of allotype kadsura longepedunculata D (g/100kg) |
| Example 1 | 16 | 58 |
| Example 2 | 15 | 56 |
| Example 3 | 15 | 55 |
| Comparative example 1 | 8 | 23 |
| Comparative example 2 | 11 | 34 |
| Comparative example 3 | 6 | 18 |
From experimental data, the compound enzyme is adopted for enzymolysis reaction, and compared with the traditional water decoction method, the method can effectively increase the yield of the Yunnan caulis Spatholobi ointment and the content of effective components. Compared with single enzyme for enzymolysis, the compound enzyme is also obviously improved, which shows that the cellulase and the alpha-amylase are jointly used and have synergistic action.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (2)
1. A method for extracting Yunnan caulis Spatholobi is characterized in that: the method comprises the following steps: taking Yunnan caulis Spatholobi as raw material, coarsely crushing, and soaking in water; adding complex enzyme for enzymolysis, inactivating, filtering to obtain Yunnan caulis Spatholobi extract, and concentrating into paste;
the compound enzyme consists of cellulase and alpha-amylase, and the mass ratio of the cellulase to the alpha-amylase is 2-4: 1; the dosage of the compound enzyme is 0.6 to 1 percent of the weight of the raw medicinal materials of the Yunnan suberect spatholobus stem; the temperature of the enzymolysis is 48-52 ℃, the pH value of the enzymolysis is 5.8-6.2, and the time of the enzymolysis is 1-3 hours.
2. The extraction method of Yunnan caulis Spatholobi according to claim 1, which is characterized in that: the amount of the added water is 4-8 times of the weight of the caulis spatholobi raw medicinal material; the soaking times are 1-5 times, and the soaking time is 1-5 hours each time.
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Citations (2)
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| CN101112415A (en) * | 2007-07-05 | 2008-01-30 | 复旦大学 | Compound milettia reticulata tablets and method for preparing the same |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102397320A (en) * | 2007-08-07 | 2012-04-04 | 北京北大维信生物科技有限公司 | Application of Chinese medicinal herb suberect spatholobus stem extract to preparation of weight-reducing and fat-reducing medicament or preparation of medicament having lipase activity inhibiting effect |
| CN104069238A (en) * | 2014-06-16 | 2014-10-01 | 南京泽朗医药科技有限公司 | Anti-tumor plant extract composition as well as preparation method and application thereof |
| CN104208156A (en) * | 2014-09-04 | 2014-12-17 | 南京标科生物科技有限公司 | Method for simultaneously extracting schisandra chinensis volatile oil and lignans |
| CN105796692A (en) * | 2016-04-25 | 2016-07-27 | 韩志强 | Waist-strengthening and kidney-invigorating tablet and preparation method thereof |
| CN108969731A (en) * | 2018-10-12 | 2018-12-11 | 广西壮族自治区药用植物园 | The preparation method for treating the Caulis Spatholobi ointment of breast cancer |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112415A (en) * | 2007-07-05 | 2008-01-30 | 复旦大学 | Compound milettia reticulata tablets and method for preparing the same |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| 复合酶法提取五味子果肉中总酚的工艺研究;张悦怡，等;《西北农林科技大学学报(自然科学版)》;20160108;第44卷(第02期);第187-192页 * |
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