CN109846923A - A kind of extracting method of Caulis Kadsurae - Google Patents
A kind of extracting method of Caulis Kadsurae Download PDFInfo
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- CN109846923A CN109846923A CN201910060938.5A CN201910060938A CN109846923A CN 109846923 A CN109846923 A CN 109846923A CN 201910060938 A CN201910060938 A CN 201910060938A CN 109846923 A CN109846923 A CN 109846923A
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- caulis kadsurae
- extracting method
- crude drug
- complex enzyme
- kadsurae
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Abstract
The invention discloses a kind of extracting methods of Caulis Kadsurae, comprising the following steps: takes Caulis Kadsurae crude drug, coarse crushing is soaked in water；Complex enzyme is added to be digested, inactivates, filtering obtains Caulis Kadsurae extracting solution, is then condensed into cream.The invention has the advantages that the yield of extract can be effectively increased, moreover it is possible to increase the content of effective component.
Description
Technical field
The invention belongs to Chinese medicine processing technique fields, and in particular to a kind of extracting method of Caulis Kadsurae.
Background technique
Caulis Spatholobi is traditional blood-activating and stasis-removing, and property is bitter, sweet, warm, returns liver and kidney channel, has replenishing and activating blood, relaxing tendons and activating collaterals
The effect of, the treatment for illnesss such as irregular menstruation, blood deficiency chlorosis, paralysis and numbness, rheumatic arthralgias.
Caulis Kadsurae is mainly derived from the rattan of Schisandraceae Kadsura Kadsura interior and Kadsura heteroclita.Yunnan
Caulis Spatholobi is the main component of compound suberect spatholobus stem pastes, when in use, is that Caulis Kadsurae is directlyed adopt to water decoction, is condensed into cream
Mode come using.In the extraction process of Caulis Kadsurae, extraction yield is few, requires further improvement.
Summary of the invention
The object of the present invention is to provide a kind of extracting methods of Caulis Kadsurae that extraction yield is high.
For achieving the above object, the technical scheme adopted by the invention is that:
A kind of extracting method of Caulis Kadsurae, comprising the following steps: take Caulis Kadsurae crude drug, coarse crushing is soaked in water；Add
Enter complex enzyme to be digested, inactivate, filtering obtains Caulis Kadsurae extracting solution, is then condensed into cream.
Preferably, described plus water dosage is 4~8 times of weight of the Caulis Kadsurae crude drug；Time of the immersion
Number is 1~5 time, and the time impregnated every time is 1~5 hour.
Preferably, the complex enzyme is made of cellulase and alpha-amylase, the cellulase and alpha-amylase
Mass ratio is 2~4:1.
Preferably, the dosage of the complex enzyme is the 0.6%~1% of the Caulis Kadsurae crude drug weight.
Preferably, the temperature of the enzymatic hydrolysis is 48 DEG C~52 DEG C, the pH of enzymatic hydrolysis is 5.8~6.2, the time 1~3 of enzymatic hydrolysis
Hour.
The present invention has following technical advantage:
Compared to the mode that traditional water decoction is boiled, the present invention is extracted using complex enzyme, can effectively increase the yield of extract,
It also can increase the content of effective component.
Specific embodiment
The technical solution in the present invention is clearly and completely described below, it is clear that described embodiment is only
Several embodiments therein of the invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment 1
A kind of extracting method of Caulis Kadsurae, comprising the following steps: take Caulis Kadsurae crude drug, coarse crushing is soaked in water；Add
Enter complex enzyme to be digested, be then heated to 100 DEG C and inactivate 8 minutes, filtering obtains Caulis Kadsurae extracting solution, is then condensed into cream.
Wherein, add the dosage of water for 6 times of weight of the Caulis Kadsurae crude drug；The number of the immersion is 3 times, every time
The time of immersion is 3 hours.
Complex enzyme is made of cellulase (10000U/g) and alpha-amylase (20000U/g), the cellulase and α-shallow lake
The mass ratio of powder enzyme is 3:1.
The dosage of complex enzyme is the 0.8% of the Caulis Kadsurae crude drug weight.
The temperature of enzymatic hydrolysis is 50 DEG C, and the pH of enzymatic hydrolysis is 6, the time 2 h of enzymatic hydrolysis.
Embodiment 2
A kind of extracting method of Caulis Kadsurae, comprising the following steps: take Caulis Kadsurae crude drug, coarse crushing is soaked in water；Add
Enter complex enzyme to be digested, be then heated to 100 DEG C and inactivate 10 minutes, filtering obtains Caulis Kadsurae extracting solution, is then condensed into
Cream.
Wherein, add the dosage of water for 8 times of weight of the Caulis Kadsurae crude drug；The number of the immersion is 5 times, every time
The time of immersion is 5 hours.
Complex enzyme is made of cellulase (10000U/g) and alpha-amylase (20000U/g), the cellulase and α-shallow lake
The mass ratio of powder enzyme is 4:1.
The dosage of complex enzyme is the 1% of the Caulis Kadsurae crude drug weight.
The temperature of enzymatic hydrolysis is 52 DEG C, and the pH of enzymatic hydrolysis is 6.2, the 3 hours time of enzymatic hydrolysis.
Embodiment 3
A kind of extracting method of Caulis Kadsurae, comprising the following steps: take Caulis Kadsurae crude drug, coarse crushing is soaked in water；Add
Enter complex enzyme to be digested, be then heated to 100 DEG C and inactivate 5 minutes, filtering obtains Caulis Kadsurae extracting solution, is then condensed into cream.
Wherein, add the dosage of water for 4 times of weight of the Caulis Kadsurae crude drug；The number of the immersion is 2 times, every time
The time of immersion is 1 hour.
Complex enzyme is made of cellulase (10000U/g) and alpha-amylase (20000U/g), the cellulase and α-shallow lake
The mass ratio of powder enzyme is 2:1.
The dosage of complex enzyme is the 0.6% of the Caulis Kadsurae crude drug weight.
The temperature of enzymatic hydrolysis is 48 DEG C, and the pH of enzymatic hydrolysis is 5.8, the 1 hour time of enzymatic hydrolysis.
Comparative example 1
Direct decocting boiling down shortens the mode of cream into.
Comparative example 2
It is digested only with cellulase, remaining is carried out fully according to the mode of embodiment 1.
Comparative example 3
It is digested only with alpha-amylase, remaining is carried out fully according to the mode of embodiment 1.
In order to verify the technical effects of the present invention, spy does following inspection, and see Table 1 for details.
The extraction yield and effective component of 1 embodiment and comparative example Caulis Kadsurae of table compare
| Project | Caulis Kadsurae cream yield (%) | Kadsura heteroclita D prime content (g/100kg) |
| Embodiment 1 | 16 | 58 |
| Embodiment 2 | 15 | 56 |
| Embodiment 3 | 15 | 55 |
| Comparative example 1 | 8 | 23 |
| Comparative example 2 | 11 | 34 |
| Comparative example 3 | 6 | 18 |
It is known that it can be effective compared to the method that traditional water decoction is boiled using complex enzyme progress enzyme digestion reaction from experimental data
Increase the yield of Caulis Kadsurae cream and the content of effective component.It is digested, is also had significant using single enzyme using complex enzyme ratio
Improvement, illustrate being used in conjunction with for cellulase and alpha-amylase, there is synergistic effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of extracting method of Caulis Kadsurae, it is characterised in that: the following steps are included: taking Caulis Kadsurae crude drug, coarse crushing adds
Water impregnates；Complex enzyme is added to be digested, inactivates, filtering obtains Caulis Kadsurae extracting solution, is then condensed into cream.
2. a kind of extracting method of Caulis Kadsurae according to claim 1, it is characterised in that: described plus water dosage is institute
State 4~8 times of weight of Caulis Kadsurae crude drug；The number of the immersion is 1~5 time, and the time impregnated every time is 1~5 hour.
3. a kind of extracting method of Caulis Kadsurae according to claim 1, it is characterised in that: the complex enzyme is by cellulose
The mass ratio of enzyme and alpha-amylase composition, the cellulase and alpha-amylase is 2~4:1.
4. a kind of extracting method of Caulis Kadsurae according to claim 1, it is characterised in that: the dosage of the complex enzyme is
The 0.6%~1% of the Caulis Kadsurae crude drug weight.
5. a kind of extracting method of Caulis Kadsurae according to claim 1, it is characterised in that: the temperature of the enzymatic hydrolysis is 48
DEG C~52 DEG C, the pH of enzymatic hydrolysis is 5.8~6.2,1~3 hour time of enzymatic hydrolysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910060938.5A CN109846923B (en) | 2019-01-23 | 2019-01-23 | Extraction method of Yunnan caulis spatholobi |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910060938.5A CN109846923B (en) | 2019-01-23 | 2019-01-23 | Extraction method of Yunnan caulis spatholobi |
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| CN109846923A true CN109846923A (en) | 2019-06-07 |
| CN109846923B CN109846923B (en) | 2021-09-17 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112415A (en) * | 2007-07-05 | 2008-01-30 | 复旦大学 | Compound milettia reticulata tablets and method for preparing the same |
| CN102397320A (en) * | 2007-08-07 | 2012-04-04 | 北京北大维信生物科技有限公司 | Application of Chinese medicinal herb suberect spatholobus stem extract to preparation of weight-reducing and fat-reducing medicament or preparation of medicament having lipase activity inhibiting effect |
| CN104069238A (en) * | 2014-06-16 | 2014-10-01 | 南京泽朗医药科技有限公司 | Anti-tumor plant extract composition as well as preparation method and application thereof |
| CN104208156A (en) * | 2014-09-04 | 2014-12-17 | 南京标科生物科技有限公司 | Method for simultaneously extracting schisandra chinensis volatile oil and lignans |
| CN105796692A (en) * | 2016-04-25 | 2016-07-27 | 韩志强 | Waist-strengthening and kidney-invigorating tablet and preparation method thereof |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
| CN108969731A (en) * | 2018-10-12 | 2018-12-11 | 广西壮族自治区药用植物园 | The preparation method for treating the Caulis Spatholobi ointment of breast cancer |
-
2019
- 2019-01-23 CN CN201910060938.5A patent/CN109846923B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112415A (en) * | 2007-07-05 | 2008-01-30 | 复旦大学 | Compound milettia reticulata tablets and method for preparing the same |
| CN102397320A (en) * | 2007-08-07 | 2012-04-04 | 北京北大维信生物科技有限公司 | Application of Chinese medicinal herb suberect spatholobus stem extract to preparation of weight-reducing and fat-reducing medicament or preparation of medicament having lipase activity inhibiting effect |
| CN104069238A (en) * | 2014-06-16 | 2014-10-01 | 南京泽朗医药科技有限公司 | Anti-tumor plant extract composition as well as preparation method and application thereof |
| CN104208156A (en) * | 2014-09-04 | 2014-12-17 | 南京标科生物科技有限公司 | Method for simultaneously extracting schisandra chinensis volatile oil and lignans |
| CN105796692A (en) * | 2016-04-25 | 2016-07-27 | 韩志强 | Waist-strengthening and kidney-invigorating tablet and preparation method thereof |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
| CN108969731A (en) * | 2018-10-12 | 2018-12-11 | 广西壮族自治区药用植物园 | The preparation method for treating the Caulis Spatholobi ointment of breast cancer |
Non-Patent Citations (6)
| Title |
|---|
| CEN Y,ET AL.: "Isolation of α-Amylase Inhibitors from Kadsura longipedunculata Using a High-Speed Counter-Current Chromatography Target Guided by Centrifugal Ultrafiltration with LC-MS", 《MOLECULES》 * |
| 刘仰斌，等: "酶解法提取鸡血藤原儿茶酸的工艺研究", 《新乡医学院学报》 * |
| 张悦怡，等: "复合酶法提取五味子果肉中总酚的工艺研究", 《西北农林科技大学学报(自然科学版)》 * |
| 张慧君，等: "响应面法优化亚麻籽粕木脂素的超声酶解提取工艺", 《食品工业》 * |
| 阴冠秀，等: "超声波辅助酶解法提取五味子乙素", 《食品科学》 * |
| 黄福荣，等: "滇鸡血藤水、醇浸出物中总木脂素含量比较", 《科技风》 * |
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