CN103520372A - Method for extracting tyrosinase inhibitor from tea seed pulp - Google Patents
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Abstract
The invention relates to an extraction method of a tyrosinase inhibitor, and aims to provide a method for extracting a tyrosinase inhibitor from tea seed pulp. The method comprises the following steps: pretreating tea seed pulp, degreasing, adding an ethanol water solution, extracting, and centrifuging to obtain filter residue and an extraction solution; adding a buffer solution into the filter residue, leaching, performing ultrasonic extraction, and centrifuging to obtain an extraction solution; performing vacuum concentration on the previous extraction solution, extracting to obtain an extraction raffinate, and performing gradient elution on the latter extraction solution with a 60% ethanol solution to obtain a 60% ethanol eluate; and mixing the extraction raffinate with the 60% ethanol eluate, concentrating, and performing freeze-drying to obtain the tyrosinase inhibitor. According to the invention, the extraction precision of the target substance is effectively improved, the extraction efficiency is greatly increased, and the economic benefits of tea seed pulp resources can be effectively increased; and the prepared tyrosinase inhibitor is a natural efficient tyrosinase inhibitor and has remarkable inhibition effect on tyrosinase.
Description
Technical field
The invention relates to the extracting method of tyrosinase inhibitor, particularly a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice.
Background technology
All kinds of mottles that melanin deposition forms have caused physiology and psychological puzzlement to modern especially women, and speckle dispelling has been the primary goal of numerous women's beauty treatments.Research shows, tryrosinase is the key enzyme that melanin forms.Tryrosinase is that a class is extensively present in the copper desmoenzyme in organism, by by tyrosine hydroxylation, produces L-DOPA (L-DOPA), and then L-DOPA is further oxidized to DOPA quinone, thereby form melanin.Meanwhile, in the brown stain that tryrosinase produces in fruit and vegerable storage and transport process, playing the part of key player, can reduce outward appearance and the quality of fruit and vegerable, thereby reduce its value.Therefore, to effectively check melanin formation of the inhibition of tryrosinase, thus the formation of all kinds of mottles of retardance human body, and can effectively prevent fruit and vegerable brown stain, keep its quality and value.
The tea-seed dregs of rice are the solid residues after tea-seed oil expression.From in December, 2009 Ministry of Public Health approval, list tea-seed oil in new food resource, tea-seed oil production scale constantly expands, and causes the output of this processing byproduct of the tea-seed dregs of rice also constantly to increase.Research shows, contains multiple bioactive ingredients such as comprising polyphenol, polysaccharide, saponin and albumen in the tea-seed dregs of rice, has high exploitation and is worth.Yet except a small amount of tea-seed dregs of rice are used as the utilization of fertilizer low value, these living resources are not also by effective exploitation at present.In recent years, from plant, extracting safe and effective natural tyrosinase inhibitor receives much concern.Yet there are no relevant report or the patent of from the tea-seed dregs of rice, extracting tyrosinase inhibitor.
Summary of the invention
Main purpose of the present invention is to overcome deficiency of the prior art, provides a kind of and can effectively utilize tea-seed dregs of rice resource, and can extract the method for natural tyrosinase inhibitor.For solving the problems of the technologies described above, solution of the present invention is:
A kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice is provided, comprises the pulverizing tea seed dregs of rice, comprise following concrete steps:
Steps A: will carry out defat after the pretreatment of the tea-seed dregs of rice, the ratio that the ethanol water of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:15ml～1g:25ml, to the ethanol water that adds volumetric concentration 95% in the tea-seed dregs of rice after defat, at 40～50 ℃, extract 30～40min, then with 12000 revs/min, after centrifugal 20 minutes, obtain filtering residue and extracting solution;
Step B: the ratio that the filtering residue that the steps A of take obtains and buffer are 1g:20ml～1g:30ml, in the filtering residue obtaining to steps A, add buffer, lixiviate 2～3h at 40～60 ℃, then after supersound extraction 10～20min, with 12000 revs/min centrifugal 20 minutes, obtain extracting solution; Described buffer is that PH is 7～9, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 3～5% after defat;
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/5～1/15 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/5～1/7 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor from the tea-seed dregs of rice.
As further improvement, the pretreatment in described steps A is that the tea-seed dregs of rice are pulverized, and then crosses 40～60 mesh sieves.
As further improvement, degreasing method in described steps A is: the ratio that is 1g:10ml～1g:20ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 1～2h at 30～50 ℃, then with 5000 revs/min, abandon supernatant after centrifugal 15 minutes, get residue and dry.
As further improvement, the vigor of the alkaline protease in described step B is not less than 20000U/g.
As further improvement, the buffer in described step B is sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution.
As further improvement, the frequency of the supersound extraction in described step B is 150～250W.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention gets effective extraction precision that has improved object indescribably by doping region, and in conjunction with supersound extraction, has greatly improved the efficiency of extracting by enzyme process, is applicable to industrialization and produces.Meanwhile, the tyrosinase inhibitor of a kind of efficiency natural of tyrosinase inhibitor of preparing in the present invention, remarkable to the inhibition of tryrosinase.In addition, the technology of the present invention can effectively improve the economic benefit of tea-seed dregs of rice resource.
Accompanying drawing explanation
Fig. 1 is the tyrosinase inhibitor that extracts in the embodiment of the present invention inhibition comparison diagram to tryrosinase.
The specific embodiment
Below in conjunction with accompanying drawing and the specific embodiment, the present invention is described in further detail:
The method concrete steps that the present invention extracts angiotensin I-converting enzyme inhibitor from tea fruit skin comprise:
Steps A: by the pretreatment of the tea-seed dregs of rice, preprocess method is that the tea-seed dregs of rice are pulverized, and then crosses 40～60 mesh sieves; After the pretreatment of the tea-seed dregs of rice, carry out defat, degreasing method is: the ratio that is 1g:10ml～1g:20ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 1～2h at 30～50 ℃, then with 5000 revs/min, abandon supernatant after centrifugal 15 minutes, get residue and dry.The ratio that the ethanol water of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:15ml～1g:25ml, to the ethanol water that adds volumetric concentration 95% in the tea-seed dregs of rice after defat, at 40～50 ℃, extract 30～40min, then with 12000 revs/min, after centrifugal 20 minutes, obtain filtering residue and extracting solution.
Step B: the ratio that the filtering residue that the steps A of take obtains and buffer are 1g:20ml～1g:30ml, in the filtering residue obtaining to steps A, add buffer, lixiviate 2～3h at 40～60 ℃, after the supersound extraction 10～20min that is 150～250W by frequency again, with 12000 revs/min centrifugal 20 minutes, obtain extracting solution.Described buffer is that PH is 7～9, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 3～5% after defat, the vigor of described alkaline protease is not less than 20000U/g, buffer preferably phosphoric acid disodium hydrogen-phosphate sodium dihydrogen buffer solution.
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/5～1/15 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/5～1/7 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor from the tea-seed dregs of rice.
The following examples can make this professional professional and technical personnel's comprehend the present invention, but do not limit the present invention in any way.
Embodiment 1
Steps A: by the pretreatment of the tea-seed dregs of rice, preprocess method is that the tea-seed dregs of rice are pulverized, and then crosses 40 mesh sieves; After the pretreatment of the tea-seed dregs of rice, carry out defat, degreasing method is: the ratio that is 1g:10ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 2h at 30 ℃, then 5000 revs/min, after centrifugal 15 minutes, abandon supernatant, get residue and dry; The ratio that the ethanol of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:15mlml, the ethanol to adding volumetric concentration 95% in the tea-seed dregs of rice after 20g defat extracts 40min at 40 ℃, then 12000 revs/min, after centrifugal 20 minutes, obtains filtering residue and extracting solution;
Step B: the ratio that the filtering residue that the steps A of take obtains and sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution are 1g:20ml, in the filtering residue obtaining to steps A, add sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, lixiviate 3h at 40 ℃, after the supersound extraction 20min that is 150W by frequency again, 12000 revs/min, centrifugal 20 minutes, obtain extracting solution; Described sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution is that PH is 7, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 3% after defat, the vigor of described alkaline protease is not less than 20000U/g;
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/5 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/5 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor 0.72g from the tea-seed dregs of rice, yield is that 3.6%(accounts for tea-seed dregs of rice dry weight after defat).
Embodiment 2
Steps A: by the pretreatment of the tea-seed dregs of rice, preprocess method is that the tea-seed dregs of rice are pulverized, and then crosses 50 mesh sieves; After the pretreatment of the tea-seed dregs of rice, carry out defat, degreasing method is: the ratio that is 1g:15ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 1.5h at 40 ℃, then 5000 revs/min, after centrifugal 15 minutes, abandon supernatant, get residue and dry; The ratio that the ethanol of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:20ml, the ethanol to adding volumetric concentration 95% in the tea-seed dregs of rice after 20g defat extracts 35min at 45 ℃, then 12000 revs/min, after centrifugal 20 minutes, obtains filtering residue and extracting solution;
Step B: the ratio that the filtering residue that the steps A of take obtains and sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution are 1g:25ml, in the filtering residue obtaining to steps A, add sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, lixiviate 2.5h at 50 ℃, after the supersound extraction 15min that is 200W by frequency again, 12000 revs/min, centrifugal 20 minutes, obtain extracting solution; Described sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution is that PH is 8, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 4% after defat, the vigor of described alkaline protease is not less than 20000U/g;
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/10 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/6 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor 0.86g from the tea-seed dregs of rice, yield is that 4.3%(accounts for tea-seed dregs of rice dry weight after defat).
Embodiment 3
Steps A: by the pretreatment of the tea-seed dregs of rice, preprocess method is that the tea-seed dregs of rice are pulverized, and then crosses 60 mesh sieves; After the pretreatment of the tea-seed dregs of rice, carry out defat, degreasing method is: the ratio that is 1g:20ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 1h at 50 ℃, then 5000 revs/min, after centrifugal 15 minutes, abandon supernatant, get residue and dry; The ratio that the ethanol of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:25ml, the ethanol to adding volumetric concentration 95% in the tea-seed dregs of rice after 20g defat extracts 30min at 50 ℃, then 12000 revs/min, after centrifugal 20 minutes, obtains filtering residue and extracting solution;
Step B: the ratio that the filtering residue that the steps A of take obtains and sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution are 1g:30ml, in the filtering residue obtaining to steps A, add sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution, lixiviate 2h at 60 ℃, after the supersound extraction 10min that is 250W by frequency again, 12000 revs/min, centrifugal 20 minutes, obtain extracting solution; Described sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution is that PH is 9, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 5% after defat, the vigor of described alkaline protease is not less than 20000U/g;
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/15 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/7 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor 0.98g from the tea-seed dregs of rice, yield is that 4.9%(accounts for tea-seed dregs of rice dry weight after defat).
The tyrosinase inhibitor of above-mentioned 3 embodiment is carried out to the checking of tryrosinase inhibition: tyrosinase inhibitor will be extracted from the tea-seed dregs of rice and be dissolved in distilled water, get the above-mentioned solution of 100 μ l, adding PH is 6.8 phosphate buffer 56 μ l and the tyrosine solution 94 μ l of 2.0mmol/L, to be mixed evenly after, in ice bath, add rapidly tryrosinase solution 50 μ l (50U), incubation 10min in 37 ℃, measures absorbance in 490nm place.
Tyrosinase inhibition rate is calculated as follows:
Tyrosinase inhibition rate (%)=[(a-b)-(c-d)]/[a-b] * 100%
A: do not add extract and absorbance that enzyme-added mixed liquor is surveyed;
B: do not add the extract absorbance that also not enzyme-added mixed liquor is surveyed;
C: add the absorbance that the mixed liquor of extract and enzyme is surveyed;
D: add extract and absorbance that not enzyme-added mixed liquor is surveyed.
As shown in Figure 1, from experimental data, can find out, the tyrosinase inhibitor that application the technology of the present invention is extracted from the tea-seed dregs of rice has significant inhibitory action to tryrosinase, and present dose-dependent effect, can be applied to the exploitation of the medicines such as beauty treatment and eliminating spot and health product, and the field such as fruit and vegerable keeping fresh and protecting color.
Claims (6)
1. from the tea-seed dregs of rice, extract a method for tyrosinase inhibitor, comprise the pulverizing tea seed dregs of rice, it is characterized in that, comprise following concrete steps:
Steps A: will carry out defat after the pretreatment of the tea-seed dregs of rice, the ratio that the ethanol water of the tea-seed dregs of rice and volumetric concentration 95% of take is 1g:15ml～1g:25ml, to the ethanol water that adds volumetric concentration 95% in the tea-seed dregs of rice after defat, at 40～50 ℃, extract 30～40min, then with 12000 revs/min, after centrifugal 20 minutes, obtain filtering residue and extracting solution;
Step B: the ratio that the filtering residue that the steps A of take obtains and buffer are 1g:20ml～1g:30ml, in the filtering residue obtaining to steps A, add buffer, lixiviate 2～3h at 40～60 ℃, then after supersound extraction 10～20min, with 12000 revs/min centrifugal 20 minutes, obtain extracting solution; Described buffer is that PH is 7～9, and containing the buffer that accounts for the alkaline protease of tea-seed dregs of rice dry weight 3～5% after defat;
Step C: the extracting solution that steps A is obtained is concentrated in vacuo to 1/5～1/15 of original volume, the ratio that the volume ratio of extracting solution after concentrating and chloroform of then take is 1:1 adds chloroform to extract in the extracting solution after concentrated, obtains raffinate;
Step D: the extracting solution that step B is obtained is concentrated in vacuo to 1/5～1/7 of original volume, cross D101 macroporous resin and carry out column chromatography, by volumetric concentration, be respectively 20%, 60% and 100% alcoholic solution and carry out gradient elution, elution flow rate is 1ml/min, collects 60% ethanol elution;
Step e: 60% ethanol elution obtaining in the raffinate obtaining in step C and step D is mixed, and concentrated postlyophilization, obtains extracting tyrosinase inhibitor from the tea-seed dregs of rice.
2. a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice according to claim 1, is characterized in that, the pretreatment in described steps A is that the tea-seed dregs of rice are pulverized, and then crosses 40～60 mesh sieves.
3. a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice according to claim 1, it is characterized in that, degreasing method in described steps A is: the ratio that is 1g:10ml～1g:20ml according to the pretreated tea-seed dregs of rice and normal hexane, in the pretreated tea-seed dregs of rice, add normal hexane, lixiviate 1～2h at 30～50 ℃, then with 5000 revs/min, abandon supernatant after centrifugal 15 minutes, get residue and dry.
4. a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice according to claim 1, is characterized in that, the vigor of the alkaline protease in described step B is not less than 20000U/g.
5. a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice according to claim 1, is characterized in that, the buffer in described step B is sodium hydrogen phosphate-phosphate sodium dihydrogen buffer solution.
6. a kind of method of extracting tyrosinase inhibitor from the tea-seed dregs of rice according to claim 1, is characterized in that, the frequency of the supersound extraction in described step B is 150～250W.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104523521A (en) * | 2014-12-19 | 2015-04-22 | 韩嘉 | Application of philippine flemingia root extract used as effective constituent for whitening |
| CN105394169A (en) * | 2015-11-08 | 2016-03-16 | 常州大学 | Compound fruit and vegetable juice/fruit and vegetable slice browning inhibitor and preparation method thereof |
| CN106420699A (en) * | 2016-09-09 | 2017-02-22 | 河南城建学院 | Novel tyrosinase inhibitor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102372761A (en) * | 2011-10-10 | 2012-03-14 | 安徽农业大学 | Method for extracting tea saponin from sasanglla cake |
| CN102860925A (en) * | 2012-08-30 | 2013-01-09 | 扬州泊瑞生物农业科技开发有限公司 | Application of theaflavin to preparation of tyrosinase inhibitor |
-
2013
- 2013-09-22 CN CN201310433236.XA patent/CN103520372B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102372761A (en) * | 2011-10-10 | 2012-03-14 | 安徽农业大学 | Method for extracting tea saponin from sasanglla cake |
| CN102860925A (en) * | 2012-08-30 | 2013-01-09 | 扬州泊瑞生物农业科技开发有限公司 | Application of theaflavin to preparation of tyrosinase inhibitor |
Non-Patent Citations (1)
| Title |
|---|
| 李敏等: "油茶籽粕中茶皂素的提取工艺研究", 《中国粮油学报》, vol. 26, no. 5, 31 May 2011 (2011-05-31), pages 38 - 42 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104523521A (en) * | 2014-12-19 | 2015-04-22 | 韩嘉 | Application of philippine flemingia root extract used as effective constituent for whitening |
| CN105394169A (en) * | 2015-11-08 | 2016-03-16 | 常州大学 | Compound fruit and vegetable juice/fruit and vegetable slice browning inhibitor and preparation method thereof |
| CN106420699A (en) * | 2016-09-09 | 2017-02-22 | 河南城建学院 | Novel tyrosinase inhibitor |
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