CN104031734A - Method for preparing sesame oil by enzymolysis and supercritical fluid extraction - Google Patents
Method for preparing sesame oil by enzymolysis and supercritical fluid extraction Download PDFInfo
- Publication number
- CN104031734A CN104031734A CN201410293037.8A CN201410293037A CN104031734A CN 104031734 A CN104031734 A CN 104031734A CN 201410293037 A CN201410293037 A CN 201410293037A CN 104031734 A CN104031734 A CN 104031734A
- Authority
- CN
- China
- Prior art keywords
- sesame
- enzymolysis
- extraction
- supercritical
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing sesame oil by enzymolysis and supercritical fluid extraction. The method comprises the following steps: (1) preparing sesame pulp, namely smashing sesame into fine powder of 100-300 meshes, adding water in 5-7 times by weight of the sesame, uniformly stirring, heating to 40-50 DEG C, and performing heat preservation for 15-20 minutes, so that the sesame pulp is obtained; (2) carrying out enzymolysis, namely regulating pH of the obtained sesame pulp to 4.0-5.0, adding compound enzyme accounting for 0.2-0.3% of the weight of the sesame pulp and carrying out enzymolysis; and (3) carrying out supercritical fluid extraction, namely placing the sesame pulp subjected to enzymolysis in step (2) into an extraction kettle, and carrying out supercritical extraction by taking supercritical CO2 fluid as an extraction solvent and an ethanol solution with volume concentration of 60-70% as an entrainer, so that the sesame oil is obtained. Compared with the prior art, the method for preparing the sesame oil by enzymolysis and supercritical fluid extraction has the advantages that oil yield is high, technology is simple, production efficiency is high and safety and reliability are realized.
Description
Technical field
The present invention relates to technical field prepared by vegetables oil, particularly relate to a kind of method of enzymolysis supercritical extraction sesame oil.
Background technology
Sesame oil, claims again sesame oil, from sesame, extract, and has special fragrance, therefore be called sesame oil.Sesame belongs to pedaliaceae flax and belongs to, and is one of China's four large oil plants, has black, white two kinds, eats with white sesame as well, medicinally take Semen Sesami Nigrum as good.The stem of sesame, leaf, pod shell, Huadu can be done medicine.Sesame is containing mineral substance and various abundant nutritive ingredients such as VITAMIN e, VITAMIN b1, linolic acid, protein, sesame candy, pentosan, calcium, phosphorus, iron.Wherein content is maximum, becomes the VITAMIN e of main component, often by people, be called and prevent old and feeble VITAMIN again, it to improving blood circulation, good effect has enhanced metabolism.The linolic acid that also has unsaturated fatty acids, it has the function of cholesterol regulating, thereby be otherwise known as " nutrition source of remaining youthful forever ".Except sesame, although it is a lot of to contain linolenic food, be all not so good as the effective of sesame.Exactly because sesame contains linolenic acid and VITAMIN e simultaneously, both exist simultaneously, have not only prevented the easily shortcoming of oxidation of linolenic acid, play again synergy, have strengthened arteriosclerosis and hypertensive result for the treatment of.Traditional Chinese medicine has higher understanding to sesame medicinal, claims that it is tonic simply, enrich blood, ease constipation, promote the production of body fluid, the effect such as logical breast, hair care, be applicable to the symptoms such as in poor health, hair color, anaemia is sallow, body fluid deficiency, constipation due to dry stool, dizziness and tinnitus.It is reported, Semen Sesami Nigrum is all effective in cure for chronic neuritis, peripheral nerve paralysis.Because sesame oil has the effect that reduces cholesterol, therefore arteriosclerosis, it is useful that hyperpietic eats.
The preparation method of sesame oil mainly contains water substitution, milling process, lixiviation process and aqueous enzymatic method, and water substitution is that hot water heating is roasted in levigate sesame to process, utilizes profit exclusion principle, and water substitutes a kind of traditional technology out by grease from oil plant.In order to obtain " perfume (or spice) " gas, improve temperature and even surpass 220 ℃, high bake sesame burns easy generation burning, and it is large to bake labor force.Milling process can bake the extracting substances that sesame causes the bad change of nutritive ingredient high temperature and focus in sesame oil, and this oil treatment has burning, also has oil anti-nutrition component.Lixiviation process is according to the similar principle that mixes, and grease and part lipid-soluble substance are invaded in organic solvent, and solution separates from insoluble residue, then from solution, reclaims solvent and obtains grease, and this method step is more, and equipment is complicated.Aqueous enzymatic method is with machinery and enzyme means are destroyed, degrading plant seed cell wall, and grease is discharged.But in aqueous enzymatic method process, form milk sap, seriously limit oil yield.How for human consumer provides, a kind of oil yield is high, nutritive ingredient is destroyed less, healthy sesame oil becomes the new demand of field, oil market.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for the enzymolysis supercritical extraction sesame oil that a kind of technique is simple, production efficiency is high, safe and reliable.
For achieving the above object, the present invention takes following technical scheme:
A method for enzymolysis supercritical extraction sesame oil, preparation method is as follows:
(1) prepare sesame slurry: it is 100～300 order fine powders that sesame is pulverized, and adds 5～7 times of water of sesame weight, stirs, and is heated to 40～50 ℃, keeps 15～20min, obtain sesame slurry;
(2) enzymolysis: the sesame slurry obtaining is regulated to pH to 4.0～5.0, add 0.2%～0.3% prozyme of sesame slurry weight, enzymolysis 1～2 hour, hydrolysis temperature is 37～47 ℃;
(3) supercritical extraction: the sesame slurries that the enzymolysis that step (2) is obtained is crossed are placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 60～70% carries out supercritical extraction as entrainment agent, and the quality of described sesame slurries and the volume of described ethanolic soln are 1:1.5～1:2; Described CO
2flow velocity is 18m/s; Described extraction temperature is 35～45 ℃, and extracting pressure is 25～35Mpa, and extraction time is 2～3 hours, obtains sesame oil.
Preferably, above-mentioned prozyme is cellulase, polygalacturonase, proteolytic enzyme, and their weight ratio is 3:2:1.
Compared with the prior art, the invention has the advantages that:
1, the present invention pulverizes sesame, and the specific surface area of sesame increases greatly, improves enzymolysis speed, shortens enzymolysis time.
2, before extraction, sesame is carried out to enzyme processing, destroy the cell walls of sesame, improve grease release rate.
3, supercritical extraction sesame oil, operational condition is gentle, and effective constituent is destroyed less, and sesame oil purity and the oil yield of proposition are high, through test, by this legal system, for sesame oil oil yield, reach 90%; Supercritical extraction is with separated two-in-one, and technique is simple, and production efficiency is high, reduces costs; CO used
2fluid is inactive gas, safe and reliable.
4, whole preparation process is all to carry out at lesser temps, compares traditional high temperature and bakes, and nutritive ingredient is not easy destroyed, and without burning.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiment.
Embodiment 1
A method for enzymolysis supercritical extraction sesame oil, comprises the steps:
(1) prepare sesame slurry: it is 100 order fine powders that sesame is pulverized, and adds 5 times of water of sesame weight, stirs, and is heated to 40 ℃, keeps 20min, obtain sesame slurry;
(2) enzymolysis: the sesame slurry obtaining is regulated to pH to 4.0, cellulase, polygalacturonase, protease composite enzyme that 0.2% weight ratio that adds sesame slurry weight is 3:2:1, enzymolysis 2 hours, hydrolysis temperature is 47 ℃;
(3) supercritical extraction: the sesame slurries that the enzymolysis that step (2) is obtained is crossed are placed in extraction kettle, the supercritical CO that the flow velocity of take is 18m/s
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 60% is entrainment agent, and the quality of sesame slurries of putting into and the volume of ethanolic soln are 1:1.5, in temperature, is 35 ℃, and extracting pressure is under 35Mpa, and supercritical extraction 2 hours, obtains sesame oil.
Embodiment 2
A method for enzymolysis supercritical extraction sesame oil, comprises the steps:
(1) prepare sesame slurry: it is 200 order fine powders that sesame is pulverized, and adds 6 times of water of sesame weight, stirs, and is heated to 45 ℃, keeps 18min, obtain sesame slurry;
(2) enzymolysis: the sesame slurry obtaining is regulated to pH to 4.5, cellulase, polygalacturonase, protease composite enzyme that 0.2% weight ratio that adds sesame slurry weight is 3:2:1, enzymolysis 3 hours, hydrolysis temperature is 42 ℃;
(3) supercritical extraction: the sesame slurries that the enzymolysis that step (2) is obtained is crossed are placed in extraction kettle, the supercritical CO that the flow velocity of take is 18m/s
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 65% is entrainment agent, and the quality of sesame slurries of putting into and the volume of ethanolic soln are 1:2, in temperature, is 40 ℃, and extracting pressure is under 30Mpa, and supercritical extraction 2.5 hours, obtains sesame oil.
Embodiment 3
A method for enzymolysis supercritical extraction sesame oil, comprises the steps:
(1) prepare sesame slurry: it is 300 order fine powders that sesame is pulverized, and adds 7 times of water of sesame weight, stirs, and is heated to 50 ℃, keeps 15min, obtain sesame slurry;
(2) enzymolysis: the sesame slurry obtaining is regulated to pH to 5.0, cellulase, polygalacturonase, protease composite enzyme that 0.3% weight ratio that adds sesame slurry weight is 3:2:1, enzymolysis 3 hours, hydrolysis temperature is 37 ℃;
(3) supercritical extraction: the sesame slurries that the enzymolysis that step (2) is obtained is crossed are placed in extraction kettle, the supercritical CO that the flow velocity of take is 18m/s
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 70% is entrainment agent, and the quality of sesame slurries of putting into and the volume of ethanolic soln are 1:2, in temperature, is 45 ℃, and extracting pressure is under 25Mpa, and supercritical extraction 2 hours, obtains sesame oil.
Claims (2)
1. a method for enzymolysis supercritical extraction sesame oil, is characterized in that, preparation process is as follows:
(1) prepare sesame slurry: it is 100～300 order fine powders that sesame is pulverized, and adds 5～7 times of water of sesame weight, stirs, and is heated to 40～50 ℃, keeps 15～20min, obtain sesame slurry;
(2) enzymolysis: the sesame slurry obtaining is regulated to pH to 4.0～5.0, add 0.2%～0.3% prozyme of sesame slurry weight, enzymolysis 2～3 hours, hydrolysis temperature is 37～47 ℃;
(3) supercritical extraction: the sesame slurries that the enzymolysis that step (2) is obtained is crossed are placed in extraction kettle, with supercritical CO
2fluid is extraction solvent, and the ethanolic soln that the volumetric concentration of take is 60～70% carries out supercritical extraction as entrainment agent, and the quality of described sesame slurries and the volume of described ethanolic soln are 1:1.5～1:2; Described CO
2flow velocity is 18m/s; Described extraction temperature is 35～45 ℃, and extracting pressure is 25～35Mpa, and extraction time is 2～3 hours, obtains sesame oil.
2. the method for enzymolysis supercritical extraction sesame oil according to claim 1, is characterized in that: described prozyme is cellulase, polygalacturonase, proteolytic enzyme, and their weight ratio is 3:2:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410293037.8A CN104031734A (en) | 2014-06-26 | 2014-06-26 | Method for preparing sesame oil by enzymolysis and supercritical fluid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410293037.8A CN104031734A (en) | 2014-06-26 | 2014-06-26 | Method for preparing sesame oil by enzymolysis and supercritical fluid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104031734A true CN104031734A (en) | 2014-09-10 |
Family
ID=51462733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410293037.8A Pending CN104031734A (en) | 2014-06-26 | 2014-06-26 | Method for preparing sesame oil by enzymolysis and supercritical fluid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104031734A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400590A (en) * | 2015-12-24 | 2016-03-16 | 兰州乾源生物科技开发有限公司 | Preparation method of bitter apricot kernel unsaturated fatty acids |
| CN106281653A (en) * | 2016-08-17 | 2017-01-04 | 江南大学 | A kind of method preparing Oleum sesami by water law breakdown of emulsion Oleum sesami body |
| CN109536273A (en) * | 2018-10-12 | 2019-03-29 | 贵州航天乌江机电设备有限责任公司 | The technique of fatty acid substance in a kind of supercritical fluid extraction sesame |
| CN113278462A (en) * | 2021-06-18 | 2021-08-20 | 汪谦 | Extraction method of sesame oil and application of sesame oil in field of skin repair |
| CN113461703A (en) * | 2021-08-16 | 2021-10-01 | 内蒙古泓兴生物科技有限公司 | Method for preparing sesamin from sesame and sesamin |
| CN113717191A (en) * | 2021-09-18 | 2021-11-30 | 安徽省凤阳县御膳油脂有限公司 | Method for preparing sesamin by using sesame seed meal |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883590A (en) * | 2006-05-24 | 2006-12-27 | 广东粤微食用菌技术有限公司 | Method for preparing ganoderma spore oil |
| CN103468392A (en) * | 2013-09-11 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Method for extracting watermelon seed oil |
| CN103789086A (en) * | 2014-02-24 | 2014-05-14 | 中华全国供销合作总社南京野生植物综合利用研究院 | Luffa cylindrica seed oil and preparation method thereof |
-
2014
- 2014-06-26 CN CN201410293037.8A patent/CN104031734A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883590A (en) * | 2006-05-24 | 2006-12-27 | 广东粤微食用菌技术有限公司 | Method for preparing ganoderma spore oil |
| CN103468392A (en) * | 2013-09-11 | 2013-12-25 | 江苏丘陵地区镇江农业科学研究所 | Method for extracting watermelon seed oil |
| CN103789086A (en) * | 2014-02-24 | 2014-05-14 | 中华全国供销合作总社南京野生植物综合利用研究院 | Luffa cylindrica seed oil and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张令莉等: "CO_2超临界萃取芝麻油中芝麻素含量的GC-MS测定", 《齐齐哈尔大学学报》 * |
| 肖杨等: "含夹带剂超临界CO_2萃取芝麻油", 《浙江化工》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400590A (en) * | 2015-12-24 | 2016-03-16 | 兰州乾源生物科技开发有限公司 | Preparation method of bitter apricot kernel unsaturated fatty acids |
| CN106281653A (en) * | 2016-08-17 | 2017-01-04 | 江南大学 | A kind of method preparing Oleum sesami by water law breakdown of emulsion Oleum sesami body |
| CN106281653B (en) * | 2016-08-17 | 2019-08-30 | 油谷生物科技南京有限公司 | A method of sesame oil is prepared by water law demulsification sesame oil body |
| CN109536273A (en) * | 2018-10-12 | 2019-03-29 | 贵州航天乌江机电设备有限责任公司 | The technique of fatty acid substance in a kind of supercritical fluid extraction sesame |
| CN113278462A (en) * | 2021-06-18 | 2021-08-20 | 汪谦 | Extraction method of sesame oil and application of sesame oil in field of skin repair |
| CN113278462B (en) * | 2021-06-18 | 2024-06-04 | 汪谦 | Sesame oil extraction method and application thereof in skin repair field |
| CN113461703A (en) * | 2021-08-16 | 2021-10-01 | 内蒙古泓兴生物科技有限公司 | Method for preparing sesamin from sesame and sesamin |
| CN113717191A (en) * | 2021-09-18 | 2021-11-30 | 安徽省凤阳县御膳油脂有限公司 | Method for preparing sesamin by using sesame seed meal |
| CN113717191B (en) * | 2021-09-18 | 2024-06-11 | 安徽省凤阳县御膳油脂有限公司 | Method for preparing sesamin by using sesame seed meal |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104031734A (en) | Method for preparing sesame oil by enzymolysis and supercritical fluid extraction | |
| CN104432388B (en) | Shiny-leaved yellowhorn peptide nutrient food and preparation method thereof | |
| CN103540493B (en) | A kind of compound method of ganoderma lucidum tea wine | |
| CN102293292B (en) | Health tea with functions of yin nourishing and blood nourishing, and processing method thereof | |
| CN105475787A (en) | Dendrobium and Radix Ophiopogonis milk beverage and preparation method thereof | |
| CN100586442C (en) | Method of producing glossy ganoderma polypeptide product | |
| CN104026263A (en) | Anti-oxidation anti-aging health-care sesame oil and preparation method thereof | |
| CN105532979A (en) | Red date lactic acid bacterium beverage and preparation method thereof | |
| CN102008411A (en) | Production formula of skin cream and preparation method of skin cream | |
| KR101914239B1 (en) | The useful for hair growth in external application composition of the manufacturing methods | |
| CN105087139A (en) | Peanut oil with effects of invigorating spleen and nourishing liver and making method thereof | |
| CN106265785A (en) | A kind of Fructus Hippophae depression capsule and production technology thereof | |
| CN104187583A (en) | Processing method for ganoderma lucidum powder | |
| CN105942093A (en) | Lotus plumule-almond protein beverage and production method thereof | |
| CN105028698A (en) | Wind and cold dispelling walnut oil | |
| KR100412785B1 (en) | Tea composition for improving circulation of the blood and manufacturing method thereof | |
| CN105104580A (en) | Wind-dispelling and cold-removal snakegourd fruit seed oil | |
| CN105028697A (en) | Wind and cold dispelling walnut oil | |
| CN103602539A (en) | Preparation method for health beer | |
| CN104996601A (en) | Walnut oil capable of expelling wind and clearing away cold | |
| CN109170047A (en) | A kind of concentration tea and preparation method thereof improving sleep and treatment constipation | |
| CN109112031A (en) | A kind of honey health preserving wine and preparation method thereof | |
| CN104026268A (en) | Health-care sesame oil for tonifying brain and quieting spirit and preparation method thereof | |
| CN102265956B (en) | Ginseng honey tea | |
| CN105941640A (en) | Honey-lotus root milk beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140910 |