CN1292280A - Functional tonifying health-care product produced by utilizing enzyme engineering and its preparation method - Google Patents
Functional tonifying health-care product produced by utilizing enzyme engineering and its preparation method Download PDFInfo
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Abstract
The persent invention provides a functional type health-care product prepared by using enzyme engineering and its preparation method. Its main raw material includes the Chinese medicinal materials of ginseng, velvet deerhorn, sea horse, dog's kidney, made silk moth, astragalus root, lycium berry and Chinese angelica root. According to the prescription of traditional Mongolian medicine said invention combines it with modern biological enzyme engineering technology to make deep processing treatment, at the same time it adopts the extraction methods of ethyl alcohol heating refluxing, aqueous vapour distillation and reduced pressure concentration, etc. so as to obtain the invented product with the obvious function for exciting potential energy of human body, raising vital function state, balancing, regulating and invigorating ondocrine and promoting blood circulation, etc.
Description
The present invention relates to a kind of nutrient health and manufacture method thereof, relate in particular to and a kind ofly utilize modern bio-enzyme engineering technology that mongolian medicine material and part Chinese herbal medicine are carried out deep processing, and a kind of natural function type nutrient health that obtains.
Mongolian medicine is a wonderful work in the Chinese medical treasure-house, and it and Chinese medicine, Tibetan medicine are listed as three big pillars of China's medicine in modern age, at enhancing physical ability and the blood aspect of fostering the spirit of nobility its unique effect is arranged more.Traditional mongolian medicine directly uses crude drug usually, or with crude drug infusion medicated wine etc., generally be single or a small amount of several raw materials, do not accomplish fully rationally utilizing of effective ingredient, and might reduce drug effect because of influencing each other between each composition, even cause having side effects.Raising along with progress of science and technology and people's living standard, consumer to the demand of health product by simple function to nutrition, health care, treatment, multi-functional direction in one such as health preserving develops, the manufacture method of above-mentioned common natural health-care products, being about to plant such as Chinese herbal medicine soaks, steaming and decocting, filter, concentrate, blend at last, perhaps animal drugs is immersed in the wine, these natural health-care products made from pure physics and/or chemical method can be subjected to bigger restriction on nutritional labeling, thereby exist absorbance low, function singleness, DeGrain or the defective that is not inconsistent with theory.So, in today of scientific and technical high speed development, excavate the mongolian medicine resource, by the infiltration of modern theory and technological means, revitalizing the mongolian medicine industry is to motherland's traditional medicine significant contribution already, and has become a new economic focus of pharmaceuticals industry development in following 10 years.
The present invention proposes under this background, promptly, the object of the present invention is to provide a kind of functional tonifying health-care product, it has been used for reference the traditional mongolian medicine prescription and in conjunction with modern biotechnology crude drug has been carried out deep processing, and it has possessed the drug effect of modern medicine when having kept the advantage of traditional mongolian medicine on the pharmacology.
Another object of the present invention has been to provide the preparation method of this functional tonifying health-care product, makes it to realize suitability for industrialized production.
At first, nutrient health provided by the invention is formed by extracting solution and Mel, the configuration of crystal sugar water of crude drug raw material, ethanol content in the final products is a 5-10% weight, described crude drug raw material comprises: Radix Ginseng 2-4 weight portion, Cornu Cervi Pantotrichum 0.5-1.5 weight portion, Hippocampus 0.5-1.5 weight portion, Testis et Pentis Canis 4-5 weight portion, Fructus Lycii 4-6 weight portion, Radix Angelicae Sinensis 2-5 part, male Bombycis mori 4-6 weight portion, Radix Astragali 2-4 weight portion, wherein:
The extracting solution of Radix Ginseng, Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis obtains through enzymolysis and ethanol extraction respectively for these crude drug, and the extracting solution of all the other crude drug is equal ethanol extract.
The present invention is that the inventor passes through to excavate and arrangement traditional mongolian medicine prescription, mixes the tcm theory in modern age, in conjunction with modern biotechnology, the mongolian medicine material is carried out that deep processing is handled and the functional tonifying health-care product made.It has not only kept the advantage of traditional mongolian medicine on the pharmacology, has possessed the drug effect of modern medicine simultaneously, is the crystallization that the traditional mongolian medicine resource combines with modern biotechnology.During above-mentioned nutrient health provided by the invention is formed, only comprised essential primary raw material, on this basis, concrete condition according to patient or consumer groups, any personage who possesses certain Chinese medicine knowledge may increase and improves effect by allocating other auxiliary material medicine into, the inventor studies through long-felt, also there are different auxiliary crude drug to allocate into, so preferably in natural material medicine composition of the present invention, also comprise and be selected from Radix Rehmanniae Preparata 4-9 weight portion, Radix Polygoni Multiflori 4-6 weight portion, Cortex Cinnamomi 0.5-1.5 weight portion, Flos Caryophylli 0.5-1.5 weight portion, Fructus Litseae 1-3 weight portion, in the auxiliary material medicine of Rhizoma Chuanxiong 2-4 weight portion partly or entirely.
As the preferred product of the present invention, except that above-mentioned raw materials, described auxiliary material medicine also can comprise other Chinese medicine, for example Poria, Flos Carthami, Herba Cistanches etc., and their total amounts in raw material are the 31-59 weight portion.
According to another object of the present invention, also provide this nutrient health manufacture method, comprise that described crude drug raw material comprises: Radix Ginseng 2-4 weight portion, Cornu Cervi Pantotrichum 0.5-1.5 weight portion, Hippocampus 0.5-1.5 weight portion, Testis et Pentis Canis 4-5 weight portion, Fructus Lycii 4-6 weight portion, Radix Angelicae Sinensis 2-5 part, male Bombycis mori 4-6 weight portion, Radix Astragali 2-4 weight portion to the enzymolysis of crude drug raw material and/or alcohol extraction process and to the mixing preparation process of extracting solution;
Described enzymolysis and/or alcohol extraction process comprise: Radix Ginseng, Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis add enzyme reaction respectively earlier, add alcohol heating reflux again, preserve extracting solution separately; Fructus Lycii is used alcohol heating reflux, preserves extracting solution; The Radix Angelicae Sinensis vapor distillation is collected fraction and volatile oil is removed in separation, and remaining distilling material alcohol heating reflux is preserved extracting solution, merges with distillation fraction and carries out concentrating under reduced pressure; Male Bombycis mori and Radix Astragali alcohol heating reflux are preserved extracting solution;
The mixing preparation process of extracting solution comprises: above-mentioned all final extracting solution are merged, add protease and carry out degradation reaction, add Mel, crystal sugar water adjusting taste then, and the ethanol content of adjusting wherein is 5-10%.
Among the preparation technology of the present invention, difference according to crude drug, requirement is classified and is processed respectively and handle, not only extracts active ingredients is more perfect, removed impurity wherein again, so the present invention has not only used the modern medicine technology, also used for reference modern biotechnology, comprise and adopt enzyme engineering the Chinese crude drug deep processing, such as cellulose in the plant, pectic substance etc., they not only the utmost point be insoluble in water, in leaching process, produce precipitation, and stopped the stripping of effective ingredient in the cell as cell wall constituent, contain protein in the animal drugs simultaneously, also can influence the character of extracting solution, for this reason, the present invention at first degrades them with enzyme, not only extract effective ingredient more fully, and with cellulose, protein etc. are transformed into micromolecular soluble saccharide, have both improved medicinal liquid clarity, have increased the content of nutritional labeling again.Simultaneously, the collagen protein in the animal drug also can obtain the rich in amino acid composition by the degraded of enzyme preparation, the also basis of having established for superior curative effect, and the employing of biological enzyme formulation is important creative place of the present invention thus.
Wherein, the selection of enzyme preparation comprises: the enzymolysis of Radix Ginseng is selected cellulase, pectase or its mixed compound enzyme for use, and the enzymolysis of Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis is selected protease for use.
According to the present invention, the described protease that is used for enzymolysis can be selected neutrality or acid protease, for example bacillus protein enzyme, papain, bromelain, mold protease, animal pepsin, pancreas are eaten protease, ficin etc. with fixed attention, in actual production, can select to use single or mixing protease.
Need to prove, to those skilled in the art, selected the enzyme preparation kind, the control of in use condition can be according to known technology complete operation easily, the temperature that comprises reaction, time, the addition of pH control and enzyme in the process, so the present invention has only provided the primary condition of finishing enzyme digestion reaction, corresponding change does not on this basis all influence practical range of the present invention, for example, under normal enzyme activity effect, the temperature of reaction is hanged down then corresponding prolongation of time, or in the optimum temperature range of this enzyme preparation effect, suitably increase the use amount (raising enzyme activity) of enzyme, enzymolysis speed is accelerated, the reaction needed time is correspondingly short.The various enzyme preparations that the present invention selects for use are industrialization product, various enzymes all have its amount ranges, the enzyme activity of considering different manufacturers or brand has various criterion, in actual production, the vigor specification of the concrete enzyme preparation of foundation is used in normal ranges or is is suitably increased and decreased the enzyme amount, does not all break away from design philosophy of the present invention.
Another key of the present invention is combining of enzyme engineering and chemical technology, considers the polarity and the Temperature Influence of composition when promptly using chemical technology, can adopt methods such as Different concentrations of alcohol reflux and hydrous water steam distillation, concentrating under reduced pressure.Owing to contain several have remarkable efficacy in many-side middle pharmaceutically active ingredient in the prescription, and by complementary action appropriate, that coordinate between these effective ingredient, help more to realize that former Mongolian medicine prescription regulates the function of each system of human body on the whole, show kidney invigorating and YANG supporting, consolidate and keep fit, slow down aging, vigorate qi and replenish the blood, invigorating brain and relieving mental uneasiness, improvement sleep, enhancing and restorative function, improve multiple efficacies such as human body immunity, and prevented that those from breaking the side effect of the single medicine of total balance of the body.
The preferred scheme according to the present invention, these health product can preferably be made in accordance with the following methods:
Radix Ginseng adds cellulase and/or pectase after soaking, 30-80 ℃ of reaction 0.5-6 hour, with the alcoholic solution reflux of 30-60% 2-10 hour, reflux temperature 50-90 ℃, residue extracted 1-4 hour once more with the ethanol liquid of same concentrations, merged and preserved twice extracting solution; Cornu Cervi Pantotrichum adds protease, 30-80 ℃ of reaction 0.5-6 hour, and with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue extracted 1-4 hour once more with the ethanol liquid of 30-60%, merged and preserved twice extracting solution; After pulverizing, Hippocampus adds protease, 30-80 ℃ of reaction 0.5-6 hour, and with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue extracted 1-4 hour once more with the ethanol liquid of 30-60%, merged and preserved twice extracting solution; Add protease after Testis et Pentis Canis maceration, the rubbing, 30-80 ℃ of reaction 0.5-6 hour, with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue is with the ethanol liquid of 30-60% reflux, extract, 1-4 hour once more, merges and preserves twice extracting solution;
Fructus Lycii usefulness 30-60% alcohol heating reflux 1-3 hour, reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, twice extracting solution merges concentrating under reduced pressure;
The Radix Angelicae Sinensis steam distillation, the collection fraction also separates and removes volatile oil, remaining distillation is used 30-60% alcohol heating reflux 1-3 hour, reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, the fraction that merges extracted twice liquid and steam distillation also carries out concentrating under reduced pressure;
The male Bombycis mori and the Radix Astragali are used alcohol heating reflux 1-3 hour of 30-60%, and reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, twice extracting solution merges concentrating under reduced pressure;
Above-mentioned all final extracting solution are merged, add protease and reacted 5-12 hour down in 30-80 ℃, the high molecular weight protein in the mixed liquor of further degrading adds an amount of Mel, crystal sugar water then, regulate the taste of product, and the ethanol content of adjusting wherein is 5-10%.The used protease of this process is not particularly limited, and is preferably various acid protease.
Vitamin E and vitamin C that the present invention also can add convention amount rise fresh-keeping and the antiseptical effect.
Deployed mixed liquor passes through filtration, homogenizing, sterilization at last, and packing becomes the oral liquid product, also can make different dosage forms such as granule, capsule, tablet by conventional method.
The method according to this invention, reflux temperature during ethanol extraction is 50-90 ℃, and preferably 50-70 ℃, this backflow can realize under normal pressure or reduced pressure, according to general operation general knowledge, required return time should be looked back-flow velocity and determines when the present invention utilized ethanol extraction, for example temperature is lower or normal pressure under back-flow velocity slow, can corresponding prolongation return time, otherwise, during higher or decompression operation, back-flow velocity is very fast relatively when temperature, and return time can correspondingly shorten.
According to preferred version of the present invention, used crude drug raw material also comprises: be selected from the auxiliary material medicine of Radix Rehmanniae Preparata 4-9 weight portion, Radix Polygoni Multiflori 4-6 weight portion, Cortex Cinnamomi 0.5-1.5 weight portion, Flos Caryophylli 0.5-1.5 weight portion, Fructus Litseae 1-3 weight portion, Rhizoma Chuanxiong 2-4 weight portion partly or entirely, and, Radix Rehmanniae Preparata, Radix Polygoni Multiflori and Fructus Lycii are mixed with extracting solution, and Cortex Cinnamomi, Flos Caryophylli, Fructus Litseae, Rhizoma Chuanxiong and Radix Angelicae Sinensis are mixed with extracting solution.
When Cortex Cinnamomi, Flos Caryophylli, Fructus Litseae, Rhizoma Chuanxiong and Radix Angelicae Sinensis use simultaneously, can mix according to the weight ratio of 1-3: 1-3: 2-4: 8-10: 5-7 in advance.
Except that above-mentioned raw material, the present invention is and alternative uses other raw material of Chinese medicine as the auxiliary material medicine, for example can further comprise Poria, Flos Carthami, Herba Cistanches etc., their total amounts in raw material are the 31-59 weight portion, and are mixed with extracting solution with the male Bombycis mori and the Radix Astragali.
Accompanying drawing 1 is the process flow diagram that function type health care product of the present invention are made.
The processing of Fig. 1 Raw medicine is processed and has been adopted three kinds of methods, is the note corresponding to technique scheme Carry, namely " enzymolysis " refers to the processing of ginseng, pilose antler, hippocampus and dog kidney, and " steam distillation " refers to The processing of the bulk drugs such as Radix Angelicae Sinensis, other bulk drug all adopts alcohol extract, in actual production can with Raw material batch job, hereby explanation.
In sum, the present invention can utilize tradition to cover by scientific and reasonable assembly and processing better Build up one's health by taking tonic kidney and enhancing body, consolidate and keep fit, delay senility, blood-enrich, invigorating brain and relieving mental uneasiness, improvement sleep, strengthen With generally acknowledged main effects such as restorative function and raising human immunological competences. So, protect as functional form Strong product, characteristics of the present invention can embody from following several respects:
1) be used for daily health caring, can strengthen physical efficiency, radiate vigour, increase the gastric secretion function, and blood Foster the spirit of nobility, help kidney Xing Yang.
2) protein, amino acid, vitamin and various trace elements are rich in the present invention, especially use natural Vegeto-animal extract modulation, nutritional labeling is more comprehensive, not only can promote human body to the suction of nutritional labeling Receive, and be conducive to regulate the allomeric function of human brain, to activating the brain vigor, excite nervous excitation, pre-Anti-senile dementia has good effect.
3) the traditional Chinese medicine theory of " strengthening the body resistance to consolidate the constitution " is thought: human body deficiency of blood essence thanks to is the source of ten thousand diseases, this The health products that invention provides have benefiting essence-blood, and the function of filling liver kidney is to strengthening body immunity, raising machine Body allomeric function state has a significant effect, and contains multiple medicinal herb components, is used for multiple cancer Disease, rheumatic arthritis, low blood pressure, cardiovascular and cerebrovascular disease, neurasthenic auxiliary therapy have comparatively Significant curative effect, especially to sexual deterioration, premature ejaculation and impotence, femal sexual function indifferences etc. are by having Take targetedly, can have obvious effect.
Further specify realization of the present invention below in conjunction with example, but practical range of the present invention is not constituted any qualification.
Embodiment 1
Radix Ginseng 30kg, soaked overnight is thinly sliced and is placed extraction pot, adds water 90L, adds cellulase 30g, pectase 30g, 45 ℃ were reacted 1 hour, added 95% ethanol 135L, were made into 50% ethanol liquid, and 55 ℃ were refluxed 3 hours, got the about 220L of extracting solution; Residue adds 50% ethanol 195L, 55 ℃ of backflows, and return time 2 hours gets the about 195L of extracting solution, and merge extractive liquid, gets 415L.
Cornu Cervi Pantotrichum 10kg places extraction pot, adds water 40L, adds papain 40ml, trypsin 100g, and 55 ℃ were reacted 1 hour, added 95% ethanol 11L, were made into 20% alcoholic solution, and 55 ℃ were refluxed 3 hours, got the about 45L of extracting solution; Residue adds 50% ethanol 145L, and 55 ℃ were refluxed 2 hours, got extracting solution 145L, and merge extractive liquid, gets 190L.
Hippocampus 10kg adds water 40L after the pulverizing, add papain 40ml, trypsin 100g, and 55 ℃ were reacted 1 hour, added 95% ethanol 21L and added water 39L, were made into 100L20% ethanol liquid, and 55 ℃ were refluxed 3 hours, got extracting solution 90L; Residue adds 50% ethanol 100L, reflux, 55 ℃ were refluxed 2 hours, extracting solution 100L, merge extracting solution 190L.
Testis et Pentis Canis 50kg soaks and rubs, and adds water 150kg, adds papain 200ml, and 55 ℃ were reacted 1 hour, and add 95% ethanol 53.3L and be made into 20% ethanol liquid, reflux, 55 ℃ were refluxed 3 hours, got extracting solution 240L, removed liquid level oil; Residue adds 50% ethanol 210L, reflux, 55 ℃ were refluxed 2 hours, extracting solution 210L, merge extracting solution 450L.
Radix Rehmanniae Preparata 90kg, Radix Polygoni Multiflori 60kg, Fructus Lycii 50kg place extraction pot, add 40% ethanol 1000L, and 55 ℃ were refluxed 2 hours, got extracting solution 800L; Residue adds 40% ethanol 1000L, and 55 ℃ were refluxed 1 hour, the about 1000L of extracting solution, merge extracting solution 1800L, concentrating under reduced pressure is to 300L.
Cortex Cinnamomi 10kg, Flos Caryophylli 10kg, Fructus Litseae 15kg, Radix Angelicae Sinensis 45kg, Rhizoma Chuanxiong 30kg, 110kg places extraction pot altogether, adds water 350L, and vapor distillation goes out volatile oil, preserves fraction and last the adding and blends in the jar; Add 95% ethanol 389L in the extraction pot residue distillation, be made into 50% ethanol liquid, 55 ℃ were refluxed 2 hours, preserved the about 630L of extracting solution; Residue adds 50% ethanol 361L, and 55 ℃ were refluxed 1 hour, preserved the about 360L of extracting solution, and twice the extracting solution and the fraction of steam distillation merge in blending jar, and concentrating under reduced pressure is to 100L.
Male Bombycis mori 50kg, Radix Astragali 30kg, other Chinese crude drug: Poria, Flos Carthami, Herba Cistanches etc. are 520kg altogether, is divided into trisection after the mixing, and every group of 200kg places extraction pot, adds 50% ethanol 1000L, and 55 ℃ were refluxed 2 hours, got extracting solution 800L; Residue adds 50% ethanol 1000L, and 55 ℃ were refluxed 1 hour, preserved the about 1000L of extracting solution, and merge extractive liquid, gets 1800L, and concentrating under reduced pressure is preserved to 200L.Repeat to do second, third group, extracting solution merge 600L.
With various extracting solution merge 2245L, add ficin 500g, bromelain 500g, papain 500ml, 55 ℃ of temperature, 8 hours, in order to the degraded macro-molecular protein; Add the 5Kg vitamin E, the 5Kg vitamin C is in order to fresh-keeping and anticorrosion; Add Mel 100L, with crystal sugar 150kg, adding distil water is adjusted to 5% with concentration of alcohol, passes through filtration, homogenizing, sterilization at last, and packing obtains the oral liquid product.
Claims (10)
1, a kind of nutrient health, extracting solution and Mel, the configuration of crystal sugar water by the crude drug raw material form, ethanol content in the final products is a 5-10% weight, described crude drug raw material comprises: Radix Ginseng 2-4 weight portion, Cornu Cervi Pantotrichum 0.5-1.5 weight portion, Hippocampus 0.5-1.5 weight portion, Testis et Pentis Canis 4-5 weight portion, Fructus Lycii 4-6 weight portion, Radix Angelicae Sinensis 2-5 part, male Bombycis mori 4-6 weight portion, Radix Astragali 2-4 weight portion, wherein:
The extracting solution of Radix Ginseng, Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis obtains through enzymolysis and ethanol extraction respectively for these crude drug, and the extracting solution of all the other crude drug is ethanol extract.
2, the nutrient health of claim 1, wherein, described crude drug raw material also comprise in the auxiliary material medicine that is selected from Radix Rehmanniae Preparata 4-9 weight portion, Radix Polygoni Multiflori 4-6 weight portion, Cortex Cinnamomi 0.5-1.5 weight portion, Flos Caryophylli 0.5-1.5 weight portion, Fructus Litseae 1-3 weight portion, Rhizoma Chuanxiong 2-4 weight portion partly or entirely.
3, the nutrient health of claim 2, wherein, this auxiliary material medicine also further comprises Poria, Flos Carthami, Herba Cistanches, their total amounts in raw material are the 31-59 weight portion.
4, a kind of nutrient health manufacture method, comprise that described crude drug raw material comprises: Radix Ginseng 2-4 weight portion, Cornu Cervi Pantotrichum 0.5-1.5 weight portion, Hippocampus 0.5-1.5 weight portion, Testis et Pentis Canis 4-5 weight portion, Fructus Lycii 4-6 weight portion, Radix Angelicae Sinensis 2-5 part, male Bombycis mori 4-6 weight portion, Radix Astragali 2-4 weight portion to the enzymolysis of crude drug raw material and/or alcohol extraction process and to the mixing preparation process of extracting solution;
Described enzymolysis and/or alcohol extraction process comprise: Radix Ginseng, Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis be the enzyme-added enzymolysis that carries out of elder generation respectively, adds alcohol heating reflux again, preserves extracting solution separately; Fructus Lycii is used alcohol heating reflux, preserves extracting solution; The Radix Angelicae Sinensis vapor distillation is collected fraction and volatile oil is removed in separation, and residue and aqueous solution alcohol heating reflux are preserved extracting solution, merges with distillation fraction and carries out concentrating under reduced pressure; Male Bombycis mori and Radix Astragali alcohol heating reflux are preserved extracting solution;
The mixing preparation process of extracting solution comprises: above-mentioned all final extracting solution are merged, add proteasome degradation, regulate taste with Mel, crystal sugar water then, and the ethanol content of adjusting wherein is 5-10%.
5, the described method of claim 4, wherein, the enzymolysis of Radix Ginseng is selected cellulase, pectase or its mixed compound enzymic preparation for use, and the enzymolysis of Cornu Cervi Pantotrichum, Hippocampus, Testis et Pentis Canis is selected protease for use.
6, the described method of claim 5, wherein, the protease that is used for enzymolysis is single or mixed enzyme.
7, claim 4,5 or 6 described methods, it comprises following process:
Radix Ginseng adds cellulase and/or pectase after soaking, 30-80 ℃ of reaction 0.5-6 hour, with the alcoholic solution reflux of 30-60% 2-10 hour, reflux temperature 50-90 ℃, residue is with the ethanol liquid of same concentrations reflux, extract, 1-4 hour once more, merges and preserves twice extracting solution; Cornu Cervi Pantotrichum adds protease, 30-80 ℃ of reaction 0.5-6 hour, and with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue was with the ethanol liquid of 30-60% reflux, extract, 1-4 hour once more, merged and preserved twice extracting solution; After pulverizing, Hippocampus adds protease, 30-80 ℃ of reaction 0.5-6 hour, and with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue was with the ethanol liquid of 30-60% reflux, extract, 1-4 hour once more, merged and preserved twice extracting solution; Add protease after Testis et Pentis Canis maceration, the rubbing, 30-80 ℃ of reaction 0.5-6 hour, with the alcoholic solution reflux of 10-30% 1-5 hour, reflux temperature 50-90 ℃, residue is with the ethanol liquid of 30-60% reflux, extract, 1-4 hour once more, merges and preserves twice extracting solution;
Fructus Lycii usefulness 30-60% alcohol heating reflux 1-3 hour, reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, twice extracting solution merges concentrating under reduced pressure;
The Radix Angelicae Sinensis steam distillation, the collection fraction also separates and removes volatile oil, residue and aqueous solution are used 30-60% alcohol heating reflux 1-3 hour, reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, the fraction of extracted twice liquid and steam distillation merges, concentrating under reduced pressure;
The male Bombycis mori and the Radix Astragali are used alcohol heating reflux 1-3 hour of 30-60%, and reflux temperature 50-90 ℃, the ethanol liquid of residue usefulness 30-60% reflux, extract, 1-2 hour once more, twice extracting solution merges concentrating under reduced pressure;
Above-mentioned all final extracting solution are merged, add protease and reacted 5-12 hour down, add Mel, crystal sugar water adjusting taste, and the ethanol content of adjusting wherein is 5-10% in 30-80 ℃.
8, the described method of claim 7, wherein, used crude drug raw material also comprises: be selected from the auxiliary material medicine of Radix Rehmanniae Preparata 4-9 weight portion, Radix Polygoni Multiflori 4-6 weight portion, Cortex Cinnamomi 0.5-1.5 weight portion, Flos Caryophylli 0.5-1.5 weight portion, Fructus Litseae 1-3 weight portion, Rhizoma Chuanxiong 2-4 weight portion partly or entirely, and, Radix Rehmanniae Preparata, Radix Polygoni Multiflori and Fructus Lycii are mixed with extracting solution, and Cortex Cinnamomi, Flos Caryophylli, Fructus Litseae, Rhizoma Chuanxiong and Radix Angelicae Sinensis are mixed with extracting solution.
9, the described method of claim 8, wherein, the weight proportion of Cortex Cinnamomi, Flos Caryophylli, Fructus Litseae, Rhizoma Chuanxiong and Radix Angelicae Sinensis is 1-3: 1-3: 2-4: 8-10: 5-7.
10, claim 6 or 7 described methods, wherein, described auxiliary material medicine also further comprises Poria, Flos Carthami, Herba Cistanches, their total amounts in raw material are the 31-59 weight portion, and are mixed with extracting solution with the male Bombycis mori and the Radix Astragali.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100341541C (en) * | 2003-03-05 | 2007-10-10 | 王光辉 | Nourishing wolfberry fruit ointment |
| CN102399665A (en) * | 2010-09-13 | 2012-04-04 | 温州士福梅花鹿养殖有限公司 | Process for preparing wine from fresh spotted deer antler through enzymolysis |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN104825757A (en) * | 2015-04-24 | 2015-08-12 | 王敬侠 | Health steeping wine and preparation method thereof |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
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- 2000-10-25 CN CN00129812A patent/CN1128630C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341541C (en) * | 2003-03-05 | 2007-10-10 | 王光辉 | Nourishing wolfberry fruit ointment |
| CN102399665A (en) * | 2010-09-13 | 2012-04-04 | 温州士福梅花鹿养殖有限公司 | Process for preparing wine from fresh spotted deer antler through enzymolysis |
| CN103173512A (en) * | 2013-03-07 | 2013-06-26 | 安徽丰润生物技术有限公司 | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
| CN104825757A (en) * | 2015-04-24 | 2015-08-12 | 王敬侠 | Health steeping wine and preparation method thereof |
| CN107595893A (en) * | 2017-08-25 | 2018-01-19 | 广西中医药大学 | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof |
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| CN1128630C (en) | 2003-11-26 |
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