CN1597690A - Enrichment method of rhodiola root glycoside compound - Google Patents
Enrichment method of rhodiola root glycoside compound Download PDFInfo
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- CN1597690A CN1597690A CN 200410040268 CN200410040268A CN1597690A CN 1597690 A CN1597690 A CN 1597690A CN 200410040268 CN200410040268 CN 200410040268 CN 200410040268 A CN200410040268 A CN 200410040268A CN 1597690 A CN1597690 A CN 1597690A
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- kirilow rhodiola
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Abstract
A process for enriching the salidroside compounds includes such steps as pulverizing rhodiola root, immersing in alcohol to obtain extract, dissolving in water, filtering, concentrating, dissolving in alcohol, adsorbing by macroreticular resin, alcohol eluting, recovering alcohol, and drying.
Description
Technical field
The present invention relates to a kind of from medicinal plant the method for extracting effective components, specifically, relate to the method for enrichment glycosides compound from Root of Kirilow Rhodiola.
Background technology
Root of Kirilow Rhodiola derives from the flower of the multiple red-spotted stonecrop of Crassulaceae rhodiola and tertia, root.The Crassulaceae Rhodida plant is perennial herb or semishrub plant, is born in high mountain crack of stone, the patana of height above sea level 3000-5000 rice.Mainly be distributed in state counties such as Hai Bei, Hai Xi, Hainan, Huang Nan, cajaput, Golog, kind has kind surplus the ancient Root of Kirilow Rhodiola of Radix Rhodiolae, rhodiola kirilowii Regel, long whip Root of Kirilow Rhodiola, Tang etc. 10.
Pick flowers every six July, dries in the shade; Nine, uproot October, section is dried.Cool in nature, it is sweet, bitter, puckery to distinguish the flavor of, and foster lung, heat-clearing, nourishing vigour are arranged, brings down a fever, the effect of sharp lung, controls pneumonia, neural paralysis disease, trachitis etc.The Tibetan medicine thinks that with root such as people's lung look, skin depth, smell have silver color dewdrop shape spot greatly, and redden autumn, and flower, fruit, all red person of seed are good.
The Root of Kirilow Rhodiola medicine is paid attention to by domestic and international the world of medicine deeply, The former Russian scholar think Root of Kirilow Rhodiola anti-hypoxia, antifatigue, cold-resistant, regulate aspect such as human immunity and all be better than genseng, Radix Et Caulis Acanthopanacis Senticosi; Qing Dynasty's Root of Kirilow Rhodiola is just granted a reward treasure to the borders minister as tribute and emperor.
Modern medicine study proves, Root of Kirilow Rhodiola mainly contains multiple flavonoid glycoside and two obedient constituents, Jujubogenin tyrosol etc., can be used as pilot, cosmonaut, frogman, high mountain worker and all are in the good medicine that the staff in the undesired environment builds up resistance.It all has tangible two-ways regulation and " adaptation source " sample effect to improving muscle power and brainwork efficient, is the ideal tonic.
Radix Rhodiolae is famous and precious medicinal plant, and China mainly is grown among the high mountain in Tibet, Sichuan, Yunnan, Xinjiang, Gansu and Jilin.It is a Crassulaceae, and rhodiola, per nnial herb mainly are used as medicine with root and stem.
Major ingredient has rhodioloside and to Jujubogenin tyrosol, and starch, protein, fat, tannin, flavonoid compound and micro-volatile oil are arranged in addition, and trace elements such as iron, zinc, tin, molybdenum, manganese.
According to the study, Root of Kirilow Rhodiola system " totipotency " newtype drug has foster lung, heat-clearing, nourishing vigour, brings down a fever, the effect of sharp lung, cures mainly diseases such as sexual disorder, diabetes, ypotension pneumonia, neural paralysis disease, trachitis.Abroad Radix Rhodiolae as " adaptogen " sample medicine, be widely used in antifatigue, anti-ageing and improve aspects such as mental and physical function.Can be used as pilot, cosmonaut, frogman, high mountain worker and all are in the good medicine that the staff in the undesired environment builds up resistance.It all has tangible dual regulation to improving muscle power and brainwork efficient, is the ideal tonic.
Contained rhodioloside and Jujubogenin tyrosol have antifatigue, anti-hypoxia, anti-microwave radiation, antitoxin and to neural system and metabolic dual regulation in the Root of Kirilow Rhodiola.But also be a kind of environmental adaptation medicine.Before more than thousand year, the Tibetan people of living on the Tibet plateau just is used as medicine with Root of Kirilow Rhodiola, in order to the strong body of body-building, the influence of opposing poor environment.Root of Kirilow Rhodiola has crucial meaning as a kind of environmental adaptation medicine on military medicine, aerospace medicine.
Except that above effect, go up record according to " herbal medicine is used in Tibet always ", Root of Kirilow Rhodiola also has promoting blood circulation and hemostasis, removing heat from the lung to relieve cough, analgesic, and ends the effect under the band.Cure mainly diseases such as hemoptysis, spitting of blood, pneumonia cough, women leukorrhea.Why does Root of Kirilow Rhodiola have anastalsis? assign to from the one-tenth of Root of Kirilow Rhodiola, have kind surplus the composition nearly tens of various physiologically actives.Wherein contained rhodioloside and Jujubogenin tyrosol are genseng type stimulant, and contained gallic acid, umbrella shape flower are done ester, Scopoletin, kaempferol etc. and had antibacterial and anti-inflammation functions, and kaempferol, Scopoletin also have cough-suppressing phlegm-dispelling functions simultaneously.The maximum composition of content is a tannin in the Root of Kirilow Rhodiola simultaneously, also claims tannin or tannic acid.Tannin is the thing that mixes up of a class polyatomic phenol, and molecular weight is big, polarity is stronger.Tannin can generate water-fast macromole throw out with protein knot is complete, thereby has convergency, also can make sclerosis of the skin, shields at mucomembranous surface, prevent too much secretion, stop excessive hemorrhage, so be used as hemostatic drug in the medical treatment.Be used for local hemorrhage clinically and be for external application, also oral administration to be ending the gastrorrhagia of stomach ulcer disease, and ulcer surface is also had the cuticula effect.Simultaneously can also treat watery diarrhea, because the cuticula interaction energy reduces secretion.Tannin can also be solidified the protoplasma of microorganism, so certain anti-microbial effect is arranged.
Chen Jijun etc. studies show that this platymiscium mainly contains fat, wax, sterol, phenoloid, flavonoid etc., and are in the majority with flavones especially.Pharmaceutical research shows that their contained rhodiosides (Salidroide), tyrosol (Tyrosol) and pyridrde are effective constituent.
" toxicological evaluation of food safety procedure " according to the health ministry promulgation, China Preventive Medicial Science Institute, Beijing Medical University, Capital Medical College have carried out the food safety toxicology test, and the result has proved nontoxic, no mutagenesis of Root of Kirilow Rhodiola and aberration inducing effect.Pharmacological testing proof Root of Kirilow Rhodiola has following main effect: 1, anti-aging effects: the old and feeble early stage mouse gavaging Root of Kirilow Rhodiola powder 1-2g/Kg that gave for 14 monthly ages, once a day, continuous 6 weeks, can obviously reduce by eight content of the lipofuscin of lipid peroxide (LPO) in blood, liver and the testis and testis, its action intensity is better than genseng, and 2, to immune influence: Root of Kirilow Rhodiola can improve mouse film chamber macrophage phagocytic function; Radix Rhodiolae polysaccharide does not have obvious influence to normal mouse peripheral white blood cell and thymic weight, but can promote normal mouse spleen lymphocyte to transform and raising NK cell killing activity.3, to cardiovascular influence: experimental results show that subcutaneous injection Radix Rhodiolae glucoside 200mg/ml at the body frog heart, frog heart shrinkage amplitude is obviously increased,, illustrate that rhodioloside has tangible cardiotonic than increasing by 128.6% before the administration; 4, to the influence of blood system: the Root of Kirilow Rhodiola crude extract hemopoietic progenitor cell of deficiency of blood disease mouse and each blood ingredient spinosity of peripheral blood are increased sharply give birth to, protection and repairing effect; 5, to the influence of endocrine system: Root of Kirilow Rhodiola can strengthen the endocrine function of rabbit suprarenal gland function, excited mouse rutting sedson and excited mouse and rat ovary; 6, radiation resistance: Root of Kirilow Rhodiola has its marrow polychromatic erythrocyte micronucleus production rate of reduction to the mouse of x-ray bombardment damage, suppresses the effect that lipid peroxide forms; 7, antineoplastic action: growth has certain restraining effect to rhodioloside to leukemia (K562), Root of Kirilow Rhodiola can suppress the speed of growth and the splitting ability of vitro culture people laryngeal cancer cell, promote glycogen synthetic, play the certain growth of preventing laryngeal cancer cell and proliferation function; 8, oxygen lack resistant function: under normal oxygen and the anoxia condition, it is oral that red scape is opened medicinal extract, can obviously improve the hypoxia-bearing capability of mouse cardiac muscle, and Root of Kirilow Rhodiola crude extract drug administration by injection can obviously improve mouse normal pressure hypoxia-bearing capability; 9, antifatigue effect: clinical pharmacology studies show that, rhodiola rosea formulated product all can improve trial volunteer's muscle power and intelligence, work capacity index, the error of performance rate is reduced, the efficient in the time of improving the literal check and correction, the reduction heart rate is opened and is made blood pressure recover normal.
The method of rhodioside compounds extraction separation mainly contains (1) and gets air-dry Root of Kirilow Rhodiola rhizome at present, pulverize, 70% alcohol reflux is 4 times in 80 ℃ of waters bath with thermostatic control, and each little extract concentrating under reduced pressure on rotary film evaporator reclaims ethanol, gets concentrated extracting solution; (2) lead acetate precipitate and separate: add 10% acetic acid lead solution in concentrated extracting solution, to the precipitation appearance, centrifugal 20min under 4000r/min removes precipitation, gets supernatant liquor; (3) n-butanol extraction: with supernatant liquor extraction 3 times, discard water layer with propyl carbinol, the reclaim under reduced pressure propyl carbinol obtains the mixture based on rhodioside.It is not high to extract rhodioside enriched substance purity with this method from Root of Kirilow Rhodiola, and organic solvent residual is arranged, and the use of Root of Kirilow Rhodiola has been formed restriction to a certain degree; Adopt microwave broken wall method from the Radix Rhodiolae rhizome, to extract rhodioside in the known technology in addition, but the pharmacological action that there is amplification technique flow process difficulty in microwave treatment and whether influences rhodioside wait further research, discussion.
Summary of the invention
The objective of the invention is to be intended to overcome above-mentioned prior art and lack limit, a kind of method of extracting glycosides compound in organic Root of Kirilow Rhodiola residual, safe and simple for process, easy and simple to handle of not having is provided.
The method of extracting glycosides compound in the Root of Kirilow Rhodiola provided by the present invention may further comprise the steps and undertaken by following order:
The preparation of a, Root of Kirilow Rhodiola medicinal extract: with 60% ethanol and the Root of Kirilow Rhodiola after suitably pulverizing place extractor by 10-20:1, soak after half an hour refluxing extraction 2 times, each 1-3 hour, after reclaiming ethanol, make Root of Kirilow Rhodiola medicinal extract;
The preparation of b, rhodioside: Root of Kirilow Rhodiola medicinal extract is dissolved in water, filters, filtrate is concentrated into paste, use 20% dissolve with ethanol, lysate is through absorption with macroporous adsorbent resin, dash after colourless with 1-5 times of water, carry out wash-out with 35% to 45% ethanol again, elutriant is through reclaiming ethanol and concentrating, promptly get the rhodioside compounds after the drying.
Above-mentioned Root of Kirilow Rhodiola is commercially available Root of Kirilow Rhodiola, and its rhodioside content is not less than 0.8% (wt);
Described water is the pure water that meets medicinal standard;
Described macroporous adsorbent resin is that granularity is the 20-60 purpose styrene type or third ethenoid resins;
The present invention compared with prior art has the following advantages:
1, no organic residual, safe: except adding ethanol, need not use additional organic solvents in whole processing method, ethanol is easy to recovery, do not have organic residual, the security of products height.
2, extraction yield height: rhodioside extraction yield height, extraction yield is at 15-25%.
3, simple for process, easy and simple to handle: this processing method is to carry out at normal temperatures and pressures, and the method simple operations is convenient.
Embodiment
Embodiment 1: the Radix Rhodiolae 1Kg after will suitably pulverizing inserts in the extractor, and add weight be 20Kg, concentration is 60% ethanol, thorough mixing stirs, soak after half an hour refluxing extraction 2 times, each return time 3 hours makes rhodioside extraction using alcohol medicinal extract after reclaiming ethanol; Medicinal extract is added dissolved in purified water, filtration, reconcentration is to paste, it with concentration 20% dissolve with ethanol, this lysate adsorbs through 20 purpose styrene type polymeric adsorbents, dash after colourless with 2 times of pure water, be that 35% ethanol carries out wash-out with concentration again, elutriant is through returning ethanol and concentrating, promptly get the rhodioside enriched substance after the vacuum-drying.
Embodiment 2: the Radix Rhodiolae 2Kg after will suitably pulverizing inserts in the extractor, and add weight be 20Kg, concentration is 60% ethanol, thorough mixing stirs, soak after half an hour refluxing extraction 2 times, each return time 2 hours makes rhodioside extraction using alcohol medicinal extract after reclaiming ethanol; Medicinal extract is added dissolved in purified water, filtration, reconcentration is to paste, it with concentration 20% dissolve with ethanol, this lysate adsorbs through 60 purposes, the third ethylene type polymeric adsorbent, dash after colourless with 5 times of pure water, be that 45% ethanol carries out wash-out with concentration again, elutriant is through returning ethanol and concentrating, promptly get the rhodioside enriched substance after the vacuum-drying.
Embodiment 3: the Radix Rhodiolae 1Kg after will suitably pulverizing inserts in the extractor, and add weight be 25Kg, concentration is 60% ethanol, thorough mixing stirs, soak after half an hour refluxing extraction 2 times, each return time 3 hours makes rhodioside extraction using alcohol medicinal extract after reclaiming ethanol; Medicinal extract is added dissolved in purified water, filtration, reconcentration is to paste, it with concentration 20% dissolve with ethanol, this lysate adsorbs through 50 purpose styrene type polymeric adsorbents, dash after colourless with 3 times of pure water, be that 35% ethanol carries out wash-out with concentration again, elutriant is through returning ethanol and concentrating, promptly get the rhodioside enriched substance after the vacuum-drying.
Claims (4)
1, a kind of enriching method of rhodioside compounds is characterized in that: may further comprise the steps and undertaken by following order:
The preparation of a, Root of Kirilow Rhodiola medicinal extract: with 60% ethanol and the Root of Kirilow Rhodiola after suitably pulverizing by 10-20: 1 places extractor, soaks after half an hour refluxing extraction 2 times, each 1-3 hour, makes Root of Kirilow Rhodiola medicinal extract behind recovery ethanol;
The preparation of b, rhodioside: Root of Kirilow Rhodiola medicinal extract is dissolved in water, filters, filtrate is concentrated into paste, use 20% dissolve with ethanol, lysate is through absorption with macroporous adsorbent resin, dash after colourless with 1-5 times of water, carry out wash-out with 35% to 45% ethanol again, elutriant is through reclaiming ethanol and concentrating, promptly get the rhodioside compounds after the drying.
2, the enriching method of rhodioside compounds according to claim 1 is characterized in that: above-mentioned Root of Kirilow Rhodiola is commercially available Root of Kirilow Rhodiola, and its rhodioside content is not less than 0.8% (wt).
3, the enriching method of rhodioside compounds according to claim 1 is characterized in that: described water is the pure water that meets medicinal standard.
4, the enriching method of rhodioside compounds according to claim 1 is characterized in that: described macroporous adsorbent resin is that granularity is the 20-60 purpose styrene type or third ethenoid resins;
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| CN 200410040268 CN1597690A (en) | 2004-07-21 | 2004-07-21 | Enrichment method of rhodiola root glycoside compound |
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| CN 200410040268 CN1597690A (en) | 2004-07-21 | 2004-07-21 | Enrichment method of rhodiola root glycoside compound |
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| CN1597690A true CN1597690A (en) | 2005-03-23 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355767C (en) * | 2005-12-22 | 2007-12-19 | 中国人民解放军第二军医大学 | Method of preparing Rhodiola sacra glycoside |
| CN100427096C (en) * | 2006-10-28 | 2008-10-22 | 江南大学 | Method for preparing suspension of rhodiola root nanoliposome |
| CN1990496B (en) * | 2005-12-29 | 2010-05-05 | 复旦大学 | Process for preparing high purity rhodiola root glycosides |
| CN101955501A (en) * | 2010-10-19 | 2011-01-26 | 北京大学 | Method for extracting and separating high-purity salidroside in pilot scale |
| CN102875614A (en) * | 2012-10-05 | 2013-01-16 | 南通四海植物精华有限公司 | Method for preparing salidroside from rose roots |
| CN102885728A (en) * | 2011-07-21 | 2013-01-23 | 新乡博凯生物技术有限公司 | Natural skincare agent material mainly containing Honeysuckle total flavonoids and preparation method of natural healthcare agent material |
-
2004
- 2004-07-21 CN CN 200410040268 patent/CN1597690A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100355767C (en) * | 2005-12-22 | 2007-12-19 | 中国人民解放军第二军医大学 | Method of preparing Rhodiola sacra glycoside |
| CN1990496B (en) * | 2005-12-29 | 2010-05-05 | 复旦大学 | Process for preparing high purity rhodiola root glycosides |
| CN100427096C (en) * | 2006-10-28 | 2008-10-22 | 江南大学 | Method for preparing suspension of rhodiola root nanoliposome |
| CN101955501A (en) * | 2010-10-19 | 2011-01-26 | 北京大学 | Method for extracting and separating high-purity salidroside in pilot scale |
| CN102885728A (en) * | 2011-07-21 | 2013-01-23 | 新乡博凯生物技术有限公司 | Natural skincare agent material mainly containing Honeysuckle total flavonoids and preparation method of natural healthcare agent material |
| CN102875614A (en) * | 2012-10-05 | 2013-01-16 | 南通四海植物精华有限公司 | Method for preparing salidroside from rose roots |
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