CN105294811A - Method for preparing ganoderma lucidum karst triterpenic acid and ganoderma lucidum karst polysaccharide with Nyingchi ganoderma lucidum karst as material - Google Patents
Method for preparing ganoderma lucidum karst triterpenic acid and ganoderma lucidum karst polysaccharide with Nyingchi ganoderma lucidum karst as material Download PDFInfo
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- CN105294811A CN105294811A CN201510610535.5A CN201510610535A CN105294811A CN 105294811 A CN105294811 A CN 105294811A CN 201510610535 A CN201510610535 A CN 201510610535A CN 105294811 A CN105294811 A CN 105294811A
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Abstract
The invention discloses a method for preparing ganoderma lucidum karst triterpenic acid and ganoderma lucidum karst polysaccharide with Nyingchi ganoderma lucidum karst as a material. The method includes the following process steps: (1) the fruiting bodies of the Nyingchi ganoderma lucidum karst are processed into granules or slices, and in a high-pressure extracting tank of supercritical CO2, the granules or slices are dynamically extracted in the supercritical CO2 in order to remove fat-soluble impurities; CO2 and a polar solvent are injected into the extracting tank, wherein the extraction pressure is 30Mpa to 45Mpa, the amount of the injected polar solvent is 12.4 to 17.8 percent in percentage by volume, the extraction temperature is 37.6DEG C to 76.8DEG C, the extraction time is 2 to 4 hours, and thereby ganoderma lucidum karst triterpenic acid extract is obtained; water which is 9 to 15 times as much as the percentage by weight of extraction residue is added into the extraction residue, wherein the temperature is 83.6DEG C to 100DEG C, the extraction time is 30 to 180 minutes, and thereby ganoderma lucidum karst polysaccharide extract is obtained; concentration and drying are carried out under 57.6DEG C, so that the ganoderma lucidum karst polysaccharide is obtained. The ganoderma lucidum karst triterpenic acid and the ganoderma lucidum karst polysaccharide contained in the Nyingchi ganoderma lucidum karst are extracted to the maximum, and thereby the resource is optimally utilized.
Description
Technical field
The present invention relates to biological pharmacy technical field, specifically, is for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material with Linzhi glossy ganoderma.
Background technology
Glossy ganoderma (formal name used at school: GanodermaLucidumKarst), profile is umbrella, cap kidney shape, semicircle or subcircular, is the sporophore of On Polyporaceae glossy ganoderma.There is invigorating QI and tranquilization, relieving cough and asthma effect, cough breathe heavily for dizzy egersis, shortness of breath and palpitation, consumptive disease.
The medicinal history having more than 2000 year in China of glossy ganoderma, by the magical treasure that successive dynasties medicine man is considered as strengthening by means of tonics, strengthens the body resistance to consolidate the constitution.2000, known Ganoderma (Ganoderma) fungi about more than 100 is planted, distributing the widest is red sesame (G.lu-cidum), secondly be purple sesame (G.japonicum), also have all hyoscines such as artist's conk (G.applana-tum), Ganoderma tsugae (G.tsugae) and book tree sesame (G.capense).Through a large amount of clinical study, glossy ganoderma is to the curative effect of the degree of having nothing in common with each other such as neurasthenia, hyperlipidaemia, coronary heart disease and angina pectoris, irregular pulse, Keshan disease, plateau uncomfortable disease, hepatitis, hemorrhagic fever, maldigestion, trachitis.Pharmacological research proves, glossy ganoderma has many-sided biological activity.
Main containing amino acid, polypeptide, protein, fungal lysozyme (fungallysozyme), and carbohydrate (reducing sugar and polysaccharide), ergosterol, triterpenes, coumarin glucoside, volatile oil, stearic acid, phenylformic acid, alkaloid, Lin Suanna Vitamin B2 Sodium Phosphate and C etc.; Spore is also containing N.F,USP MANNITOL, trehalose (trehalose).
REISH is that light brown, brown are to puce.Mycelia is dispersed in or bonds agglomerating, and colourless or light brown is elongated, slightly bending, has branch, diameter 2.5 ~ 6.5 microns.Spore brown, avette, top is truncate, and outer wall is colourless, and inwall has verruca, long 8 ~ 12 microns, wide 5 ~ 8 microns.
The efficacy effect that glossy ganoderma possesses:
Protecting liver and detoxication: glossy ganoderma has provide protection to the liver injury that multiple physics and chemistry and biotic factor cause.No matter before liver damage occurs or after generation, take glossy ganoderma and all can protect liver, alleviate liver injury.Glossy ganoderma can also promote that liver is to the metabolism of medicine, poisonous substance, has definite curative effect for toxic hepatitis.Especially chronic hepatitis, glossy ganoderma obviously can eliminate the symptoms such as dizziness, weak, nauseating, uncomfortable liver area, and effectively can improve liver function, makes indices be tending towards normal.So glossy ganoderma can be used for treating chronic poisoning, all kinds of chronic hepatitis, liver cirrhosis, hepatic insufficiency.
Treatment diabetes: the hypoglycemic principle of glossy ganoderma is owing to promoting the utilization of tissue to sugar.Regular Insulin can be replaced after taking glossy ganoderma and suppress disengaging of lipid acid, the symptom such as blood sugar, glucose in urine can be improved.Water-soluble polysaccharide in glossy ganoderma, can alleviate the occurring degree of non insulin dependent diabetes.
Improve cardiovascular systems: experimentation on animals and clinical trial all show; glossy ganoderma can coronary artery dilator effectively; increase coronary flow; improve Myocardial Microcirculation; strengthen the supply of myocardium oxygen and energy; therefore, to myocardial ischemia, there is provide protection, treatment and the prevention of coronary heart diseases and angina pectoris etc. can be widely used in.To high fat of blood patient, glossy ganoderma can obviously reduce blood cholesterol, lipoprotein and triglyceride, and the formation of energy prevention of arterial atherosclerotic plaque.For atherosclerotic plaque former, then there are reduction Arterial Cholesterol content, vessel softening, prevent the effect of damage further.And can local microcirculation be improved, stop platelet aggregation.These effects have good preventive and therapeutic effect for polytype apoplexy.
Skin whitening: LI Shi-Zhen just points out in Compendium of Material Medica that glossy ganoderma has " increasing wisdom, good color ", glossy ganoderma is that one has crease-resistant, anti-inflammatory, removing color spot, protection skin.Glossy ganoderma contains the composition of check melanin and the multiple trace element etc. to skin beneficiating, these compositions can by reducing human free radical, accelerate the regeneration of cell, increase the thickness of skin and increase collagen, reach plump skin, eliminate the effect of microgroove and wrinkle.Polysaccharide component wherein by keeping and regulation of skin water-based, can recover skin elasticity, making skin wet, exquisiteness.
Glossy ganoderma pharmaceutical use:
Nature and flavor: warm in nature, lightly seasoned.
Proterties: gas is special, mildly bitter flavor is puckery.
Function cures mainly
1, have no peace of mind, insomnia, palpitation with fear.The sweet property of this taste is put down, and enters the heart channel of Hang-Shaoyin, can bushing blood, the beneficial motive, tranquilizing mind, thus availablely control insufficiency of vital energy and blood, the mind lose support caused to have no peace of mind, have a sleepless night, palpitate with fear, dreaminess, forgetful, the tired god of body is tired, eat the disease such as less.Alonely can grind into powder and swallow, or be equal to Radix Angelicae Sinensis, the root of herbaceous peony, Spina Date Seed, Semen Platycladi, longan aril and use.
2, cough that to breathe heavily phlegm many.This taste is sweet can mend, and the flat temperature partially of property, enters lung channel, replenishing and restoring lung-QI, warming lung and reducing phlegm, relieving cough and asthma, often can control phlegm retention syndrome, sees the cold cough of shape, phlegm many asthma person, especially to phlegm-damp type or asthenic cold type curative effect better.Can be alone or astringe the lung with the beneficial gas such as Radix Codonopsis, shizandra berry, rhizoma zingiberis, the tuber of pinellia, warming yang for resolving fluid retention medicine is with using.
3, consumptive disease.These product have invigorating QI and blood effect, therefore conventional control the diseases such as consumptive disease is losed heart, anorexia, cold hands and feet or irritated dry, and the qi-restoratives medicine compatibilities such as normal and skunk bush, ginseng, glutinous rehmannia, as purple sesame ball (" General Medical Collection of Royal Benevolence ").
Pharmacological action
1, to the effect of central nervous system: all can reduce spontaneous activity in mice during alcohol extract abdominal injection after glossy ganoderma tincture, glossy ganoderma fermentation concentrated solution, mycelium alcohol extract and spore powder degreasing.
2, to the effect of cardiovascular systems: glossy ganoderma tincture has cardiotonic to Isolated Toad Heart, the red sesame tincture of rabbit abdominal injection can make contractile force strengthen, changes in heart rate is little, abdominal injection glossy ganoderma alcohol extract, the rabbit ECG T wave caused Pituitrin is towering obvious antagonistic action, shows that it has certain antagonism to acute myocardial ischemia.
3, platelet aggregation-against and anti thrombotic action: Ganoderma Ludicum Injection has obvious restraining effect to ADP and collagen-induced human platelet aggregation in vitro, Healthy People takes purple sesame 0.2 gram totally 1 week every day, also there is obvious suppression to the platelet aggregation of ADP induction, act on poor to collagen-induced person.Myocardial infarction or Patients with Cerebral Infarction take glossy ganoderma 2 week, the platelet aggregation that internal and external test all can suppress ADP to induce, and also have obvious restraining effect after taking medicine to ex vivo thrombosis.
4, to the effect of respiratory system: the experiment of mouse tracheae PSP proves, glossy ganoderma alcohol extract and glossy ganoderma (Ganodermasp.) fermented liquid abdominal injection all have phlegm-dispelling functions.
5, on the impact of metabolism and endocrine function: mouse peritoneal injection ganoderan BN3C(and D6) protein of serum, liver and marrow and the synthesis of nucleic acid can be promoted, the division of accelerated bone myelocyte, propagation, the content of mouse liver even slurry cell P 1 can also be increased, improve the function of detoxification of liver.Ganoderma extractum can increase the content of blood plasma cortisol to scalding animal.
6, hepatoprotective effect: mouse gavaging glossy ganoderma tincture can alleviate the liver pathological lesion caused by carbon tetrachloride poisoning.
7, to experimental myotonic effect: mouse peritoneal injection Ganoderma Ludicum Injection, has to the experimental myotony that 2,4 dichlorophenoxyacetic acid causes the effect obviously reducing zymohexase.
8, anti-oxidant, delaying senility function: ganoderan GLA, GLB, GLC all have restraining effect to the generation of superoxide anion (O) free radical and erythrocyte lipid peroxidation, and have scavenging(action) to hydroxyl radical free radical (OH), having superoxide-dismutase sample active, is the important factor that glossy ganoderma delays senility.
9, anti-inflammatory action: the artificial and natural glossy ganoderma decocting wine of rats gavaged sinks enriched material and all can dramatically and suppress that carrageenin is joint caused to swell.
10, antitumor action: Ganoderma sporophore handle crude extract reaches 87.6% to mouse S180 inhibiting rate, its composition is containing polysaccharide and protein.Be separated to obtain multiple polysaccharide abdominal injection in glossy ganoderma hot water extractives, have restraining effect to mouse S180 transplanted tumor.Oral then invalid.
11, immunoregulation effect: the protein-polysaccharide abdominal injection be separated in glossy ganoderma can make the cell in mouse peritoneal transudate, scavenger cell, polymorphonuclear leukocyte increase, show there is immune strengthening effect, obviously increase the spleen Plaque forming cell number of sheep red blood cell (SRBC) (SRBC) immune mouse, this kind of polysaccharide is containing semi-lactosi, glucose and xylose.
Linzhi glossy ganoderma place of production scope is Tibet Autonomous Region's Nyingchi Prefecture's Nyingchi County, Milin County, Gongbujiangda country, Bowo County, Chayu County, Lang County, Motuo County Xian Xia administrative region, totally 7 counties.
It has following characteristic:
1. sense organ characteristic: stem is shorter, in dark red, having paint-like light, is white sponge in stem; The rounded shape of cap, surface redness or scarlet, edge color is slightly light, and there is paint-like light at center before not dusting, periphery is white, and after dusting, cap is covered by spore powder, matt, faint yellow.
2. physical and chemical index:
Wild product and cultivation product: ganoderma lucidum crude polysaccharide content >=1%, Ganoderma triterpenoids >=1.5%, adenosine >=7.2%.
3. safety and other quality technologies require: product safety and other quality technologies require to meet national relevant regulations.
Ganoderma triterpenoids acid is the class triterpene compound found in glossy ganoderma, and that is triterpene compound is one of the primary chemical and effective component of glossy ganoderma.In glossy ganoderma, contained triterpene compound has significant especially physiologically active.In the society of high competition, overwrought work, living-pattern preservation or because of environmental pollution, cause the grievous injury to HUMAN HEALTH, triterpene compound has huge using value.
Show the research of glossy ganoderma physiological and biochemical procedure, triterpene compound (Ganodenic acid) belongs to the secondary metabolism thing of glossy ganoderma.Find in glossy ganoderma physiological ecological and the research life history, mycelium, by after mycelia, secondary mycelia to three mycelia, could produce sporophore by knot.The pre-structure material of enough triterpene compounds (Ganodenic acid) can only be accumulated between mycelium period, then could fruiting (namely transferring sexual propagation to by vegetative propagation), and produce sporophore.In the ripening process of sporophore, it is perfect that triterpene substance is just able to biological chemical combination, becomes the triterpenes components with physiologically active.Can say: ganoderma lucidum triterpene is Ganoderma mycelium I and II meta-bolites, so the product that every glossy ganoderma mycelium fermentation is made, due to not through sporophore later stage---ripening process, triterpene compound content does not almost have or extremely small.Meanwhile, in prematurity sporophore, triterpene compound content is also few, only has ripe of the right age sporophore, and namely grow the sporophore of l ~ 2 month, triterpene compound content is just more.Here it is why not with product or the preparation of glossy ganoderma, the distinct essence reason of its Costco Wholesale, quality, effect.
Due to singularity and the physiological function widely thereof of ganoderma lucidum triterpene compounds, the healthy and nutritive food association identification triterpenes of Japan is only the important indicator judging ganoderma lucidum prod quality.
Glossy ganoderma (spore) play anti-inflammatory, analgesia, calmness, anti-ageing, poisoning tumour cell, anti-hypoxia etc. effect main composition, experiment proves that ganoderma lucidum triterpene has the effect improving rapidly immunizing power, simultaneously can be difficult to the G0 phase tumour cell that kills by activated chemotherapy medicine, carry out separately and combine poisoning.
Human body tumour cell 60% is proliferative cell, the basis of be tumor development, grow up, shifting, very big to harm, but responsive to chemicotherapy, easily kills; 40% is G0 phase cell, cry again resting stage cell, do not divide, do not grow, but insensitive to chemicotherapy, be difficult to be killed by medicines such as chemotherapy.When some factor makes the mortality of proliferation period cell (during as chemotherapy), G0 phase tumour cell can enter proliferating cycle, becomes the root of tumor recurrence.The maximum feature of Ganoderma triterpenoids shows as and can be difficult to the G0 phase tumour cell that kills by activated chemotherapy medicine, orders about it and enters phase of cell growth, then combined chemotherapy or kill it separately, amount reproduction and transfer after preventing it from terminating resting stage.Ganoderma triterpenoids is to the unique effect of G0 phase cell.
Various countries are in the research of glossy ganoderma, and about Ganoderma sporophore, mycelial chemical composition, and Ganoderma triterpenoids (i.e. Ganodenic acid) research is the most detailed, and structure clearly widely can be utilized by modern science and medical science, and Ganoderma triterpenoids is the weathervane of glossy ganoderma inhibiting cancer research.World Ganoderma triterpenoids researcher has delivered increasing paper, prove that triterpene compound is the main pharmacological components of glossy ganoderma, that glossy ganoderma plays anti-inflammatory, analgesia, calm, anti-ageing, poisoning tumour cell, the main composition experiment of the effect such as anti-hypoxia proves that ganoderma lucidum triterpene has the effect improving rapidly immunizing power, show promotion lymphopoiesis, improve scavenger cell, NK cell, the phagocytic activity of T cell and lethality, and directly and indirectly kill tumour cell, simultaneously can be difficult to the G0 phase tumour cell that kills by activated chemotherapy medicine, carry out independent and combine poisoning.
Ganoderma triterpenoids is to the unique effect of G0 phase cell, and effectively checking Malignant Tumor Recurrence transfer, compensate for the deficiency of traditional tumour treatment (operation, radiotherapy, chemotherapy), becomes the main force in immunotherapy of tumors.
Summary of the invention
The object of the invention is to design with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, super critical CO 2 technology is adopted to extract Ganoderma triterpenoids acid and extracted by polysaccharide component contained in raffinate, reach maximized to extract Ganoderma triterpenoids acid and ganoderan contained by Linzhi glossy ganoderma, make resource obtain optimized utilization.
The present invention is achieved through the following technical solutions: with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, comprise following processing step:
1) Linzhi's Ganoderma sporophore is processed into granular or flap, then raw material is put into supercritical CO
2high-pressure extracting tank in, inject a little polar solvent and soak raw material, in 30 ~ 45Mpa, the supercritical CO at 37.6 ~ 76.8 DEG C
2dynamic extraction 18 ~ 25min, to remove oil-soluble impurities;
2) in extractor, CO is injected
2and polar solvent, extraction pressure is 30 ~ 45Mpa, and polar solvent injection rate by volume per-cent is 12.4 ~ 17.8%, and Extracting temperature is 37.6 ~ 76.8 DEG C, 2 ~ 4 hours extraction times, obtains Ganoderma triterpenoids acid extraction liquid;
3) through step 2) after, add the water of its mass percent 9 ~ 15 times to raffinate, 83.6 ~ 100 DEG C, extraction time 30 ~ 180min, obtains ganoderma lucidum polysaccharide extract;
4) described ganoderma lucidum polysaccharide extract less than 57.6 DEG C is concentrated and dry, obtain ganoderan.
Further to better implement the present invention, the following set-up mode of special employing: described CO
2purity be 99.9 ~ 99.999%.
Further to better implement the present invention, the following set-up mode of special employing: step 3) can be carried out in high-pressure extracting tank or common extractor.
Further to better implement the present invention, the following set-up mode of special employing: in step 4), described concentrated referring to drying is concentrated into liquid specific gravity 1.042 ~ 1.078 time, and input flash dryer carries out drying.
Further to better implement the present invention, the following set-up mode of special employing: described flash dryer is spray-dryer.
Further to better implement the present invention, the following set-up mode of special employing: described polar solvent is volume percent 92.6 ~ 98.7% ethanol, methyl alcohol or 100% ethyl acetate.
Further to better implement the present invention, the following set-up mode of special employing: in step 3), water used is that tap water, distilled water are or/and pure water.
The present invention compared with prior art, has the following advantages and beneficial effect:
The present invention adopts super critical CO 2 technology extract Ganoderma triterpenoids acid and extracted by polysaccharide component contained in raffinate, reach maximized to extract Ganoderma triterpenoids acid and ganoderan contained by Linzhi glossy ganoderma, make resource obtain optimized utilization.
Preparation method of the present invention, shows through studying for a long period of time, and the Ganoderma triterpenoids acid of extracting and ganoderan are one to three times of prior art acquisition rate.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1:
With Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, comprise following processing step:
1) Linzhi's Ganoderma sporophore is processed into granular or flap, then raw material is put into supercritical CO
2high-pressure extracting tank in, inject a little polar solvent and soak raw material, in 30 ~ 45Mpa, the supercritical CO at 37.6 ~ 76.8 DEG C
2dynamic extraction 18 ~ 25min, to remove oil-soluble impurities;
2) in extractor, CO is injected
2and polar solvent, extraction pressure is 30 ~ 45Mpa, and polar solvent injection rate by volume per-cent is 12.4 ~ 17.8%, and Extracting temperature is 37.6 ~ 76.8 DEG C, 2 ~ 4 hours extraction times, obtains Ganoderma triterpenoids acid extraction liquid;
3) through step 2) after, add the water of its mass percent 9 ~ 15 times to raffinate, 83.6 ~ 100 DEG C, extraction time 30 ~ 180min, obtains ganoderma lucidum polysaccharide extract;
4) described ganoderma lucidum polysaccharide extract less than 57.6 DEG C is concentrated and dry, obtain ganoderan.
Embodiment 2:
The present embodiment is at the enterprising one-step optimization in the basis of above-described embodiment, further to better implement the present invention, and the following set-up mode of special employing: described CO
2purity be 99.9 ~ 99.999%.
Embodiment 3:
The present embodiment is at the enterprising one-step optimization in the basis of above-mentioned any embodiment, further to better implement the present invention, and the following set-up mode of special employing: step 3) can be carried out in high-pressure extracting tank or common extractor.
Embodiment 4:
The present embodiment is at the enterprising one-step optimization in the basis of above-mentioned any embodiment, further to better implement the present invention, the following set-up mode of special employing: in step 4), described concentrated referring to drying is concentrated into liquid specific gravity 1.042 ~ 1.078 time, and input flash dryer carries out drying.
Embodiment 5:
The present embodiment is at the enterprising one-step optimization in the basis of above-described embodiment, further to better implement the present invention, and the following set-up mode of special employing: described flash dryer is spray-dryer.
Embodiment 6:
The present embodiment is at the enterprising one-step optimization in the basis of above-mentioned any embodiment, further to better implement the present invention, and the following set-up mode of special employing: described polar solvent is volume percent 92.6 ~ 98.7% ethanol, methyl alcohol or 100% ethyl acetate.
Embodiment 7:
The present embodiment is at the enterprising one-step optimization in the basis of above-mentioned any embodiment, further to better implement the present invention, and the following set-up mode of special employing: in step 3), water used is that tap water, distilled water are or/and pure water.
The present invention adopts super critical CO 2 technology extract Ganoderma triterpenoids acid and extracted by polysaccharide component contained in raffinate, reach maximized to extract Ganoderma triterpenoids acid and ganoderan contained by Linzhi glossy ganoderma, make resource obtain optimized utilization.
The above is only preferred embodiment of the present invention, and not do any pro forma restriction to the present invention, every any simple modification, equivalent variations done above embodiment according to technical spirit of the present invention, all falls within protection scope of the present invention.
Claims (7)
1. with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: comprise following processing step:
1) Linzhi's Ganoderma sporophore is processed into granular or flap, then raw material is put into supercritical CO
2high-pressure extracting tank in, inject a little polar solvent and soak raw material, in 30 ~ 45Mpa, the supercritical CO at 37.6 ~ 76.8 DEG C
2dynamic extraction 18 ~ 25min, to remove oil-soluble impurities;
2) in extractor, CO is injected
2and polar solvent, extraction pressure is 30 ~ 45Mpa, and polar solvent injection rate by volume per-cent is 12.4 ~ 17.8%, and Extracting temperature is 37.6 ~ 76.8 DEG C, 2 ~ 4 hours extraction times, obtains Ganoderma triterpenoids acid extraction liquid;
3) through step 2) after, add the water of its mass percent 9 ~ 15 times to raffinate, 83.6 ~ 100 DEG C, extraction time 30 ~ 180min, obtains ganoderma lucidum polysaccharide extract;
4) described ganoderma lucidum polysaccharide extract less than 57.6 DEG C is concentrated and dry, obtain ganoderan.
2. according to claim 1 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: described CO
2purity be 99.9 ~ 99.999%.
3. according to claim 1 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: step 3) can be carried out in high-pressure extracting tank or common extractor.
4. according to claim 1 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: in step 4), described concentrated referring to drying is concentrated into liquid specific gravity 1.042 ~ 1.078 time, and input flash dryer carries out drying.
5. according to claim 4 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: described flash dryer is spray-dryer.
6. according to any one of claim 1-5 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: described polar solvent is volume percent 92.6 ~ 98.7% ethanol, methyl alcohol or 100% ethyl acetate.
7. according to any one of claim 1-5 with Linzhi glossy ganoderma for the method for Linzhi's triterpenic acid and ganoderan prepared by starting material, it is characterized in that: in step 3), water used is that tap water, distilled water are or/and pure water.
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Cited By (5)
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| CN106581083A (en) * | 2016-12-07 | 2017-04-26 | 盐城工学院 | Extraction method for ganoderma lucidum components, biological feed and preparation method thereof |
| CN107652371A (en) * | 2017-09-29 | 2018-02-02 | 福建绿宝食品集团有限公司 | A kind of preparation method of Ganoderma lucidum extract |
| CN108395487A (en) * | 2018-05-07 | 2018-08-14 | 福建仙芝楼生物科技有限公司 | A kind of preparation method and its equipment of the ganoderma lucidum polysaccharide that antitumor activity is high |
| CN109134584A (en) * | 2018-08-14 | 2019-01-04 | 四川兴杰象药业有限公司 | A kind of preparation method for separating and purifying extracting Corosolic acid from loguat leaf |
| CN111662393A (en) * | 2020-07-01 | 2020-09-15 | 广东悦生生物科技有限公司 | Method for extracting total components of lucid ganoderma |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106581083A (en) * | 2016-12-07 | 2017-04-26 | 盐城工学院 | Extraction method for ganoderma lucidum components, biological feed and preparation method thereof |
| CN107652371A (en) * | 2017-09-29 | 2018-02-02 | 福建绿宝食品集团有限公司 | A kind of preparation method of Ganoderma lucidum extract |
| CN108395487A (en) * | 2018-05-07 | 2018-08-14 | 福建仙芝楼生物科技有限公司 | A kind of preparation method and its equipment of the ganoderma lucidum polysaccharide that antitumor activity is high |
| CN108395487B (en) * | 2018-05-07 | 2022-12-27 | 福建仙芝楼生物科技有限公司 | Preparation method and equipment of ganoderma lucidum polysaccharide with high antitumor activity |
| CN109134584A (en) * | 2018-08-14 | 2019-01-04 | 四川兴杰象药业有限公司 | A kind of preparation method for separating and purifying extracting Corosolic acid from loguat leaf |
| CN111662393A (en) * | 2020-07-01 | 2020-09-15 | 广东悦生生物科技有限公司 | Method for extracting total components of lucid ganoderma |
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Application publication date: 20160203 |