CN101327237A - Composition containing plant effective component and preparation method and use thereof - Google Patents
Composition containing plant effective component and preparation method and use thereof Download PDFInfo
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Abstract
The invention discloses a plant active component composite containing ginseng polysaccharide, total saponins of panax ginseng, and hydrolysate of ginsenoside. The invention also discloses a preparation method thereof and an application thereof for regulating the immunity of the organism and resisting tumor.
Description
Technical field
The present invention relates to the compositions of a kind of plant active component, especially relate to a kind of composition and method of making the same and application of Radix Ginseng active component.
Technical background
Along with the quickening of socioeconomic prosperity, rhythm of life, the fierceness day by day of competition, modern particularly middle age will bear the dual heavy burden of family and work, very big variation has taken place in the factor that influences health, causes multiple disease to such an extent as to muscle power, the mental work of long-term over loading cause body's immunity to descend.
Modern medicine, cytobiology and development of molecular biology, make people recognize that immune disorder not only can produce multiple disease, and substantial connection is all arranged with the generation of human senility and old people's frequently-occurring disease such as tumor, hypertension, diabetes, immunologic function has the rheological properties of increasing and changes, and promptly the age increases its immunologic function degression or disorder, atrophy of thymus gland as a result, the T cell depletion, thus body aging caused, the lost of life.And the Chinese medicine immunomodulator can strengthen old people's immunologic function, closely during the last ten years, chemists have obtained multiple chemical compound from tonification class Chinese medicines such as strengthening the body resistance, not only can promote body's immunological function, and have confirmed to have really very strong immunocompetence.Therefore searching has good immunoregulation effect, with the medicine of multiple diseases such as prevention and treatment tumor, cardiovascular and cerebrovascular vessel.
The Chinese medicine Radix Ginseng is the dry root of Araliaceae Radix Ginseng (Panax ginseng C.A.Mey), and sweet, the little hardship of nature and flavor is flat.Have that strongly invigorating primordial QI, multiple arteries and veins take off admittedly, invigorating the spleen to benefit the lung, effect such as promote the production of body fluid, calm the nerves.China's pharmacy ancient books and records Shennong's Herbal the earliest claims that " main tonifying five ZANG-organs is pacified spirit to Radix Ginseng, decides soul, and spasmolytic is throbbed with fear, and eyesight-improving intelligence-developing is obeyed to make light of one's life by commiting suicide for a long time and prolonged life." afterwards, in medical books such as " Mingyi Bielu ", treatise on Febrile Diseases, Tang Materia Medica and Compendium of Material Medica, detailed record is arranged all." property of medicine opinion " says its " main the five internal organs deficiency, five kinds of strain and seven kinds of impairment, deficient thin and weak ", and all lung spleens lose empty, deficient qi and blood.The disease of body fluid consume all can be received good tonification effect.Pharmacological research shows that the effect of Radix Ginseng mainly shows the following aspects:
One, can regulate the central nervous system, improve the excitement and the process of inhibition of brain, make it to tend to balance.Can improve mental and blue-collar ability, increase work efficiency, and antifatigue effect is arranged.
Two, can promote the utilization of brain, improve ability of learning and memory energy matter.
Three, can increase myocardium power, decreased heart rate increases cardiac output and coronary flow, but ischemia resisting and arrhythmia.
Four, has the effect that strengthens sexual function and promote gonad function and growth.
But five human body immunity improving power.
Six, improve defensive ability/resistance ability to destructive stimulus, but the stress ability of enhancing body and adaptability.
Seven, can improve hemopoietic function.
Eight, also have many-sided effect such as radioprotective, antiviral, antitumor, shock.
Modern medicine is through chemical analysis, and Radix Ginseng comprises multiple composition, organic acid, vitamin, saccharide, inorganic salt, sterin, oligopeptide, polysaccharide, volatile oil and ginsenoside.Studies show that the main medicinal active ingredient of Radix Ginseng is exactly ginsenoside and ginseng polysaccharide, as ginsenoside Ro, Rb1, Rb2, Rc, Rd, Re, Rg1 etc.A large number of experiments show that the ginseng polysaccharide has tangible immunoregulation effect, the ginsenoside shows obvious antineoplastic.But studies show that multiple naturally occurring ginsenoside such as Rb1, Rg3 all can produce Rh in the Radix Ginseng under the metabolism of intestinal microbial population
2, (ginsenoside Rh
2Be a kind of in the contained glycol saponins of Radix Ginseng, the very little blood circulation that enters of content), this shows, the ginsenoside Rh
2It might be the real active component of ginsenoside.A large amount of researchs also confirm, the ginsenoside Rh
2Have clear and definite antitumor action, as inducing tumor cell break up again, tumor-inhibiting action, immunoregulation effect etc. in the multidrug resistance phenomenon of inducing apoptosis of tumour cell reversing tumor cell, body.
The medicine that contains the ginseng effective component at present all is that a kind of composition with the ginseng effective component is used as medicine, and as ginsenoside or ginseng polysaccharide, does not give full play of the function curative effect of Radix Ginseng.Mend principle according to the phase patibhaga-nimitta of Chinese medicine, ginseng polysaccharide, ginsenoside and ginsenoside Rh2 with clear and definite curative effect are united use, bring into play the function curative effect of Chinese medicine better.
Summary of the invention
The object of the present invention is to provide a kind of plant active component compositions and a preparation thereof.
Another object of the present invention is to provide described preparation of compositions method.
A further object of the invention is to provide described compositions in preparation enhancing immunity, the application on antitumor drug or the health product.
The present invention adopts the ginseng polysaccharide by a large amount of experiment sievings, forms compositions or makes preparation with ginsenoside's catabolite compatibility in the Radix Ginseng total saponins.
The present invention is implemented by following technical proposals.
Compositions of the present invention, form by following component by weight percentage:
Ginsenoside's catabolite 0.1～14%,
The ginseng polysaccharide 68.0～98.0%,
Radix Ginseng total saponins 1.9～18.0%.
Compositions of the present invention, preferably form by following component by weight percentage:
Ginsenoside's catabolite 0.5～11%,
The ginseng polysaccharide 83～95%,
Radix Ginseng total saponins 4～15%.
Compositions of the present invention, most preferably form by following component by weight percentage:
Ginsenoside's catabolite 0.8～4%,
The ginseng polysaccharide 90～94.5%,
Radix Ginseng total saponins 4.5～10%
Compositions of the present invention, the best are made up of following component by weight percentage:
Ginsenoside's catabolite 1%
The ginseng polysaccharide 93.7%
Radix Ginseng total saponins 5.3%
According to compositions of the present invention, wherein the ginseng polysaccharide includes but not limited to: one or more in Radix Ginseng polysaccharide, Stem and leaf of Radix Ginseng polysaccharide, Flos Ginseng's polysaccharide, the Herba Herminii polysaccharide, its preparation method is with suitable water or low-concentration ethanol in Radix Ginseng, stem and leaf, flower or the fruit one or more to be decocted, extracting solution concentrates, through the pure repeated precipitation of height, filter, drying and other steps obtains ginseng polysaccharide's extractum.Removing the preparation method that adopts ginseng polysaccharide of the present invention also can utilize the preparation method of present technology to obtain the ginseng polysaccharide, for example utilize ultrasonic extraction ginseng polysaccharides' such as Chinese patent application CN1580078A, CN1634122A, CN1669558A, CN1821275A, Zhong Zhensheng research, [chemistry and biological engineering, 2006 (6)] etc. preparation method obtains the ginseng polysaccharide, can also purchase the ginseng polysaccharide on market.
Compositions according to the present invention, wherein Radix Ginseng total saponins includes but not limited to: one or more in stem and leaf of Radix Ginseng saponin, Radix Ginseng saponin, Herba Herminii saponin, the Flos Ginseng's saponin, its preparation method is that water or certain density ethanol are to one or more extractions in Radix Ginseng, stem and leaf, flower or the fruit, extracting solution is through concentrating precipitation, filtration, column chromatography, the dry ginsenoside's extractum that gets.Except that adopting said method, can also unite the use several different methods, utilize the preparation method of prior art to obtain the ginsenoside, for example utilize and permitted [the ultrasound wave rapid extracting method research of ginseng total saponins such as the Chinese-English people of grade, physical and chemical inspection and chemical fascicle, 2002 (12)] [Chinese patent medicine 2001 such as Cai Xiong, 12 (9)], [forest chemical engineering communication 2005 such as high rosy clouds, 12 (5)], Zhang Chunhong etc., the comparative study of conventional water extraction method of ginsenoside and new process for extracting, Chinese crude drug, 2006 (10) etc. method obtain the ginsenoside, can also on market, buy Radix Ginseng total saponins.
Compositions according to the present invention, wherein ginsenoside's catabolite includes but not limited to that alkaline degradation, acid degradation, Smith degraded, heating hydrolysis or enzymatic degradation obtain, and the raw material ginsenoside of degraded can be one or more in stem and leaf of Radix Ginseng saponin, Radix Ginseng saponin, Herba Herminii saponin or the Flos Ginseng's saponin.
Its preparation method is: add acetic acid solution among the ginsenoside, heating, a kind of in stirring, filtration, extraction, column chromatography, drying or unite and use several different methods to be prepared into ginsenoside's catabolite; Add sodium hydroxide solution among the ginsenoside, heating, a kind of in stirring, filtration, extraction, column chromatography, drying or unite and use several different methods to be prepared into ginsenoside's catabolite; Ginsenoside and hydrolytic enzyme Mixed culture connect bacterium, a kind of in concussion, centrifugal, precipitation, dialysis, drying or unite and use several different methods to be prepared into ginsenoside's catabolite.Ginsenoside's catabolite contains but is not limited to following chemical compound as ginsenoside Rh1, ginsenoside Rh2, ginsenoside Rk2, dammarane type-20 (21), 24-diene-3 β, 12 beta-diols, ginsenoside Rg3, preferably ginseng's saponin Rh
2
Radix Ginseng of the present invention includes but not limited to: wild ginseng, Park Ginseng, Radix Ginseng, Radix Ginseng Rubra, Korean Ginseng.
Patent documentation of being quoted or non-patent literature herein, all in full as a reference.
According to method of the present invention prepare compositions by weight percentage among the ginseng polysaccharide polysaccharide component content be not less than 65%, saponin constituent content is not less than 80% in the Radix Ginseng total saponins, Ginseng secondary glycoside Rh in ginsenoside's catabolite
2Content is not less than 5%.And no matter to obtain by prior art for preparing still be to buy in market, and its purity does not reach above-mentioned content standard, then should carry out exquisiteness, makes it to meet above-mentioned content standard, can use as the raw material of compositions.
Compositions of the present invention can be made certain dosage form in conjunction with various carriers such as excipient or adjuvant, and wherein the dosage form of preparation is divided into oral, intramuscular, intraperitoneal, subcutaneous or intravenous administration.Oral agents is divided into conventional tablet, the quick releasing formulation of capsule, drop pill, granule, oral liquid, soft capsule, oral cavity disintegration tablet, powder, unguentum, sublimed preparation, pill, suppository and these dosage forms thereof or durative action preparation, powder spray, aerosol, gel.Subcutaneous or intravenously administrable is divided into injection, lyophilized injectable powder etc.
Compositions of the present invention can also be as one of effective ingredient of other drug or health product.
Beneficial effect provided by the present invention
To the comparative study of different proportioning pharmacodynamics.
One, compositions enhancing immunity experiment
The compositions (counting by weight percentage) that obtains according to the preparation method of embodiment 1
Compositions 1 ginseng polysaccharide 93.7%+ Radix Ginseng total saponins 5.3%+ ginsenoside catabolite 1%
Compositions 2 ginseng polysaccharide 83%+ Radix Ginseng total saponins 10%+ ginsenoside catabolites 7%
Compositions 3 ginseng polysaccharide 74%+ Radix Ginseng total saponins 15%+ ginsenoside catabolites 11%
Compositions 4 ginseng polysaccharide 68%+ Radix Ginseng total saponins 18%+ ginsenoside catabolites 14%
After per os gave the compositions of different proportionings, each treated animal vegetative activity was all normal, had carried out antibody-producting cell test, HD50 pH-value determination pH, delayed allergy respectively.Concrete test method is as follows:
Get 180 of the female secondary kunming mices of 17-22g, provide by Institute of Experimental Animals, Chinese Academy of Medical Sciences's breeding field.180 are divided into is 3 experimental grouies, 60 every group.Each experimental group 60 mice is divided into 5 groups, and 12 every group, 4 groups of compositionss that give different proportionings respectively wherein, another group compares.One group of mice of immunity carries out the antibody-producting cell test; Two groups of mices of immunity carry out the HD50 pH-value determination pH; Three groups of mice delayed allergy experiments of immunity.
Compositions is configured to solution with distilled water, and per os is irritated stomach, and matched group gives the distilled water of equivalent, once a day, gives 20-28 days continuously, measures every index after the last administration in 24 hours.
The different proportioning compositionss of table 1.1 are to the influence (means standard deviation) of mice delayed allergy (the foot system thickens method)
| Group | Number of animals | Toes thickness difference (mm) before and after attacking | The P value |
| Matched group | 12 | 0.28±0.16 | - |
| Compositions 1 | 12 | 0.41±0.18 | 0.047 |
| Compositions 2 | 12 | 0.49+0.20 | 0.036 |
| Compositions 3 | 12 | 0.57±0.17 | 0.00 |
| Compositions 4 | 12 | 0.52±0.15 | 0.00 |
By table 1.1 as seen, the toes thickness difference all was higher than matched group before and after each proportioning compositions group mice was attacked, and with the matched group comparing difference significance (P＜0.05) was arranged all.
The different proportioning compositionss of table 1.2 are to the influence (means standard deviation) of mice delayed allergy (ear swelling method)
| Group | Number of animals | Toes thickness difference (mm) before and after attacking | The P value |
| Matched group | 12 | 14.1±7.9 | - |
| Compositions 1 | 12 | 18.1±5.3 | 0.047 |
| Compositions 2 | 12 | 18.7±9.3 | 0.036 |
| Compositions 3 | 12 | 22.0±6.8 | 0.00 |
| Compositions 4 | 12 | 21.5±7.3 | 0.00 |
By table 1.2 as seen, each proportioning compositions group mice left and right sides ear weight difference all is higher than matched group, and with the matched group comparing difference significance (P＜0.05) is arranged all.
The different proportioning compositionss of table 2 are to mice half hemolysis value (HC
50) influence (means standard deviation)
| Group | Number of animals | HC 50 | The P value |
| Matched group | 12 | 117.43±35.9 | - |
| Compositions 1 | 12 | 119.72±50.67 | 0.875 |
| Compositions 2 | 12 | 120.36±29.55 | 0.768 |
| Compositions 3 | 12 | 131.59±47.39 | 0.427 |
| Compositions 4 | 12 | 128.79±26.31 | 0.579 |
By table 2 as seen, each proportioning compositions group mice half hemolysis value (HC
50) all be higher than matched group.
The different proportioning compositionss of table 3 are to the influence (means standard deviation) of mouse antibodies cellulation experiment
| Group | Number of animals | Hemolysis plaque number (* 10 3/ full spleen) | The P value |
| Matched group | 12 | 113.5±49.3 | - |
| Compositions 1 | 12 | 160.5±75.2 | 0.042 |
| Compositions 2 | 12 | 145.3±58.9 | 0.393 |
| Compositions 3 | 12 | 175.6±62.3 | 0.000 |
| Compositions 4 | 12 | 188.0±63.5 | 0.000 |
By table 3 as seen, each proportioning compositions group mouse antibodies cellulation number average is higher than matched group.
Experimental result shows that each proportioning compositions all is positive to the influence of mice half hemolysis value and antibody-producting cell, proves that compositions to the humoral immunization adjusting effectively; Each proportioning compositions must influence all the mice delayed allergy and be positive, and proves that the immunomodulating of compositions pair cell is effective.
Conclusion: the present composition has immunoregulation effect.
Two, the experiment of compositions function of tumor inhibition:
The compositions (counting by weight percentage) that obtains according to the preparation method of embodiment 1
Compositions 1 ginseng polysaccharide 93.7%+ Radix Ginseng total saponins 5.3%+ ginsenoside catabolite 1%
Compositions 2 ginseng polysaccharide 83%+ Radix Ginseng total saponins 10%+ ginsenoside catabolites 7%
Compositions 3 ginseng polysaccharide 74%+ Radix Ginseng total saponins 15%+ ginsenoside catabolites 11%
Compositions 4 ginseng polysaccharide 68%+ Radix Ginseng total saponins 18%+ ginsenoside catabolites 14%
After per os gave the compositions of different proportionings, each treated animal vegetative activity was all normal, carried out tumor growth inhibition test and life respectively and prolonged test.
60 of experiment 1:S-180 sarcoma laboratory mices, body weight 18-22 gram, Military Medical Science Institute's Experimental Animal Center provides.Under aseptic condition, inoculation S-180 oncocyte is subcutaneous in the right axil of mice in mouse peritoneal, every inoculation oncocyte 3 * 10
6Individual.Tumor-bearing mice is divided into 5 groups at random, 12 every group, 4 groups of compositionss that give different proportionings respectively wherein, another group compares.Inoculate 24 hours gastric infusions, once a day, each is organized administration time and is 27 days, peels off the tumor piece on the 28th day, weighs, and calculates inhibition rate of tumor growth.
The different proportioning compositionss of table 4 are to the influence (means standard deviation) of mice S-180 tumor cell growth experiment
| Group | Number of animals | Average tumor heavy (g) | Suppression ratio |
| Normal saline | 12 | 2.62±1.50 | - |
| Compositions 1 | 12 | 1.33±0.59 | 49.38 * |
| Compositions 2 | 12 | 1.62±1.12 | 30.12 |
| Compositions 3 | 12 | 1.43±1.09 | 45.87 * |
| Compositions 4 | 12 | 1.48±1.59 | 40.36 * |
*Expression P value＜0.05
By table 4 as seen, different proportioning compositionss all have inhibitory action to the growth of mice S-180 tumor.
75 of experiment 2:S-180 sarcoma laboratory mices, body weight 18-22 gram, Military Medical Science Institute's Experimental Animal Center provides.Under aseptic condition, inoculation H-22 ehrlich ascites carcinoma oncocyte is inoculated oncocyte 1 * 10 for every in mouse peritoneal in mouse peritoneal
7Individual.Tumor-bearing mice is divided into 5 groups at random, 15 every group, 4 groups of compositionss that give different proportionings respectively wherein, another group compares.Inoculate 24 hours gastric infusions, once a day, each organizes administration time till dead mouse.Calculate and respectively organize the mean survival time, calculate increase in life span.
The different proportioning compositionss of table 5 are to mice H-22 ehrlich ascites carcinoma pharmacodynamic observation (means standard deviation)
| Group | Number of animals | Mean survival time (my god) | Increase in life span (%) |
| Normal saline | 15 | 18.21±0.58 | - |
| Compositions 1 | 15 | 24.00±0.85 | 10.38 * |
| Compositions 2 | 15 | 21.31±0.63 | 9.57 |
| Compositions 3 | 15 | 20.85±0.51 | 9.06 * |
| Compositions 4 | 15 | 21.17±0.76 | 9.32 |
*Expression P value＜0.05
By table 5 as seen, different proportioning compositionss all can prolong the trophophase of H-22 ehrlich ascites carcinoma mice.
When the reference specific embodiment is described the present invention, embodiment does not attempt in office where face limits the scope of the invention, and can carry out multiple variation and modification to these embodiments simultaneously.
Specific embodiment
The preparation method of mixture material:
Embodiment 1
Get Radix Ginseng 500g, adding distil water decocts 3 times, and amount of water is respectively 3000ml, 2500ml, 2500ml, and decocting time was respectively 2 hours, and 2 hours, 1.5 hours.Merge extractive liquid, is concentrated into 800ml, and adding 95% ethanol, to make determining alcohol be 80%.With sedimentation and filtration, after water redissolved, making determining alcohol at adding 95% ethanol was 70%, and with sedimentation and filtration, vacuum drying promptly gets ginseng polysaccharide 20.4g, and polyoses content is 75.8% in anthrone-this ginseng polysaccharide of sulphuric acid determination of color.
Get Radix Ginseng 500g, add 75% alcohol reflux 3 times, 75% ethanol consumption is respectively 3000ml, 2500ml, 2500ml, and return time was respectively 2 hours, and 2 hours, 1.5 hours.Merge extractive liquid,, and be evaporated to nothing alcohol flavor, with D on the concentrated solution
101Macroporous adsorbent resin, wash 5 times of column volumes after, with 60% ethanol elution and collect, concentrate eluant is to doing.Get Radix Ginseng total saponins 42.8 grams.With saponin content in vanillin-this Radix Ginseng total saponins of perchloric acid determination of color is 89.5%.
Get ginsenoside's 50 grams, add sodium hydroxide solution 300ml, stir evenly, be heated to boiling.And kept 1.5 hours, in degraded solutions, adding isopyknic distilled water, the limit edged stirs, and adds the back standing over night.Get precipitation, drying.Get ginsenoside's catabolite 18.6 grams.Record Rh in this ginsenoside's catabolite with HPLC
2Content be 7.6%.
Embodiment 2
Get Radix Ginseng 500g, cross 80～100 mesh sieves, soak and spend the night, dry, extract or reflux, extract, at least 5 times, with the medicinal residues volatilization to the greatest extent behind the ethanol with 75～80% alcohol dipping, soak, be heated to boiling, keep little extraction of boiling, extract altogether 5 times, merge extractive liquid, is concentrated into 1/10 volume, add 95% ethanol or dehydrated alcohol, to alcoholic degree be 40%, centrifugal 15 minutes of 3000rpm discards precipitation, supernatant continues to add 95% ethanol or dehydrated alcohol, to determining alcohol be 60%, concentrate the back and add 95% ethanol or dehydrated alcohol, to determining alcohol be 80%, centrifugal 15 minutes of 3000rpm, collecting precipitation embathes 1～2 time with dehydrated alcohol, obtains ginseng polysaccharide 18.7g.
Get Radix Ginseng 1000g, with 75% alcohol reflux 4 times, at every turn with 8 times of amount alcohol reflux 1.5 hours, united extraction night, filter filtrate recycling ethanol, concentration is concentrated into relative density 1.05 (60 ℃ of mensuration), is chilled to room temperature, measures extracted with diethyl ether 3 times with 1/4, discard ether solution, water boils, and flings to residual ether, filters, filtrate is chilled to room temperature, with water saturated equal-volume sec-butyl alcohol extraction 4 times, merge the sec-butyl alcohol extract, with 1/4 water washing of measuring 2 times, discard water liquid, the reclaim under reduced pressure sec-butyl alcohol is concentrated into relative density 1.05～1.10 (60 ℃ of mensuration), adds ethanol and makes concentration of alcohol reach 40%, stir, cold preservation is spent the night, and filters, and filtrate concentrates, drying promptly gets ginsenoside 105g.
Get ginsenoside's 50 grams, add acetic acid solution 300ml, stir evenly, be heated to boiling.And kept 1.5 hours, in degraded solutions, adding isopyknic distilled water, the limit edged stirs, and adds the back standing over night.Get precipitation, drying.Promptly get ginsenoside's catabolite 18.0 grams, standby.
Embodiment 3
Get Radix Ginseng 250g, decoct with water 3 times, each 2 hours, merge extractive liquid,, extracting solution is removed ethanol extremely to the greatest extent with 75% precipitation, precipitation drying, the drying precipitated water dissolution of using, after the dissolving fully, cold preservation is spent the night and is got supernatant concentration to relative density about 1.2 (60 ℃ of mensuration), adds the 200g polyamide and mixes drying thoroughly, be loaded in the chromatographic column, the water eluting is collected eluent, is concentrated into relative density 1.2 (60 ℃ of mensuration), adds ethanol and makes concentration of alcohol reach 80%, stir, left standstill 12 hours, and filtered and get precipitation, after absolute ethanol washing precipitation 2～3 times, vacuum drying promptly gets ginseng polysaccharide 15.6g, and is standby.
Get Radix Ginseng 250g, with 70% alcohol reflux three times, each 2 hours, merge extractive liquid,, and be evaporated to and do not have the alcohol flavor, with D on the concentrated solution
101Macroporous adsorbent resin, wash 3 times of column volumes after, with 65% ethanol elution and collect, concentrate eluant is to doing, and is standby.
Get ginsenoside's 50 grams, adding hydrolytic enzyme 3g mixes and makes culture medium, connects bacterium, and 3 days, the centrifugal thalline of removing are cultivated in 30 ℃ of concussions, add the saturated ammonium sulfate centrifugal collecting precipitation in the supernatant, gets enzymatic degradation product 19.3 grams.
Embodiment 4
According to the preparation method of the ginseng polysaccharide of embodiment 1, ginsenoside, Radix Ginseng catabolite, corresponding quantity is enlarged 3 times, promptly get the raw material of compositions of the present invention.
Embodiment 5
With the raw material of embodiment 4, get ginseng polysaccharide 90g, Radix Ginseng total saponins 9.2g, ginsenoside's catabolite 0.8g mix homogeneously gets the present composition.
Embodiment 6
With the raw material of embodiment 4, get ginseng polysaccharide 98g, Radix Ginseng total saponins 1.9g, ginsenoside's catabolite 0.1g mix homogeneously gets the present composition.
Embodiment 7
With the raw material of embodiment 4, get ginseng polysaccharide 68g, Radix Ginseng total saponins 18g, ginsenoside's catabolite 14g, mix homogeneously gets the present composition.
Embodiment 8
Raw material with embodiment 4.Get ginseng polysaccharide 94.5g, Radix Ginseng total saponins 4g, ginsenoside's catabolite 1.5g, mix homogeneously obtains the present composition.
Embodiment 9
With the raw material of embodiment 4, get ginseng polysaccharide 95g, Radix Ginseng total saponins 4.5g, behind ginsenoside's catabolite 0.5g mix homogeneously, add 1.5 times of amount dextrin, 0.5% sucrose, 1.5% microcrystalline Cellulose, make soft material with an amount of alcoholic solution, granulate, 60 ℃ of forced air dryings are granulated, granulate promptly gets granule.
Embodiment 10
With the raw material of embodiment 4, get ginseng polysaccharide 93.7g, Radix Ginseng total saponins 5.3g, behind ginsenoside's catabolite 1g mix homogeneously, add 5% crosslinked adding 5% polyvinylpolypyrrolidone, 0.1% magnesium stearate, 50% microcrystalline Cellulose, make soft material with an amount of alcoholic solution, granulate 60 ℃ of forced air dryings, granulate, granulate, compacting promptly gets oral cavity disintegration tablet in flakes.
Embodiment 11
Get the present composition 100g of embodiment 5-8, add equivalent starch, granulate, incapsulate, promptly get capsule.
Embodiment 12
Get the present composition 400g of embodiment 5-8, add 400mgPEG, mix homogeneously, fusion, last drop pill machine is made drop pill.
Embodiment 13
Get the present composition 230 grams of embodiment 5-8 respectively, mannitol 90 grams, EDTA15 gram and distilled water 15ml, behind the said components mix homogeneously, lyophilization, 1000 of packing.
Claims (12)
1, a kind of compositions is characterized in that: said composition is made up of ginseng polysaccharide, Radix Ginseng total saponins and ginsenoside's catabolite.
2, a kind of compositions as claimed in claim 1, it is characterized in that: said composition is made up of following component by weight percentage:
Ginsenoside's catabolite 0.1～14%,
The ginseng polysaccharide 68.0～98.0%,
Radix Ginseng total saponins 1.9～18.0%.
3, a kind of compositions as claimed in claim 2, wherein
Ginsenoside's catabolite 0.5～11%,
The ginseng polysaccharide 83～95%,
Radix Ginseng total saponins 4～15%.
4, as claim 3 described a kind of compositionss, wherein
Ginsenoside's catabolite 0.8～4%,
The ginseng polysaccharide 90～94.5%,
Radix Ginseng total saponins 4.5～10%.
5, a kind of compositions as claimed in claim 4, wherein
Ginsenoside's catabolite 1%
The ginseng polysaccharide 93.7%
Radix Ginseng total saponins 5.3%.
6, any described a kind of preparation of compositions method of claim 1～5, step is as follows:
A ginseng polysaccharide's preparation: get Radix Ginseng, decoct with suitable water or low-concentration ethanol, extracting solution concentrates, and through the pure repeated precipitation of height, filters, and drying obtains the ginseng polysaccharide;
The preparation of B Radix Ginseng total saponins: get Radix Ginseng, water or certain density ethanol extraction, extracting solution is through concentrating precipitation, filtration, column chromatography, the dry Radix Ginseng total saponins that gets;
The preparation of C ginsenoside catabolite: get Radix Ginseng total saponins, with alkaline degradation, acid degradation or enzymatic degradation any one or unite the product that uses several different methods to obtain:
D preparation of compositions: the ginseng polysaccharide in the steps A, Radix Ginseng total saponins among the step B and the ginsenoside's catabolite mix homogeneously among the step C are got final product.
7, a kind of preparation of compositions method as claimed in claim 6 is characterized in that the preparation method of ginsenoside's catabolite among the described step C is:
Acid degradation: get the ginsenoside that step B obtains, add acetic acid solution, heating is through stirring, filtration, extraction, column chromatography, the dry ginsenoside's catabolite that gets;
Alkaline degradation: get the ginsenoside that step B obtains, middle adding sodium hydroxide solution, heating is through stirring, filtration, extraction, column chromatography, the dry ginsenoside's catabolite that gets; Perhaps
Enzymatic degradation: get the ginsenoside and the hydrolytic enzyme Mixed culture that obtain among the step B, connect bacterium, through concussion, centrifugal, precipitation, dialysis, dry ginsenoside's catabolite.
8, as any compositions in the claim 1～5, it is characterized in that: polysaccharide component content is not less than 65% by weight percentage among the described ginseng polysaccharide, saponin constituent content is not less than 80% by weight percentage in the Radix Ginseng total saponins, Ginseng secondary glycoside Rh in ginsenoside's catabolite
2Content is not less than 5% by weight percentage.
9, a kind of preparation of compositions method as claimed in claim 6, it is characterized in that: polysaccharide component content is not less than 65% by weight percentage among the steps A acquisition ginseng polysaccharide, obtain among the step B that saponin constituent content is not less than 80% by weight percentage in the Radix Ginseng total saponins, Ginseng secondary glycoside Rh in ginsenoside's catabolite during step C obtains
2Content is not less than 5% by weight percentage.
10, make pharmaceutically acceptable preparation by any compositions in the claim 1～5.
11, any compositions is preparing enhancing immunity, the application on antitumor drug or the health product in the claim 1～5.
12, the described preparation of claim 10 is in preparation enhancing immunity, the application on antitumor drug or the health product.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101113242A CN101327237A (en) | 2007-06-18 | 2007-06-18 | Composition containing plant effective component and preparation method and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101113242A CN101327237A (en) | 2007-06-18 | 2007-06-18 | Composition containing plant effective component and preparation method and use thereof |
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| CN101327237A true CN101327237A (en) | 2008-12-24 |
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| CNA2007101113242A Pending CN101327237A (en) | 2007-06-18 | 2007-06-18 | Composition containing plant effective component and preparation method and use thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695513B (en) * | 2009-10-28 | 2011-12-14 | 上海永神生物科技有限公司 | Composition with anti-tumor effect and application thereof |
| CN102058647B (en) * | 2009-11-17 | 2014-05-14 | 天士力制药集团股份有限公司 | Ginseng glycoside H extract and preparation method thereof |
| CN107752001A (en) * | 2017-10-15 | 2018-03-06 | 长春中医药大学 | A kind of ginseng small molecule oral liquid and preparation method thereof |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN110973432A (en) * | 2019-08-20 | 2020-04-10 | 泰州医药城国科化物生物医药科技有限公司 | Ginseng solid beverage and preparation method thereof |
| CN115260269A (en) * | 2022-06-28 | 2022-11-01 | 成都大学 | Composition containing ginseng glucoside and aglycone thereof, preparation method and application |
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2007
- 2007-06-18 CN CNA2007101113242A patent/CN101327237A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695513B (en) * | 2009-10-28 | 2011-12-14 | 上海永神生物科技有限公司 | Composition with anti-tumor effect and application thereof |
| CN102058647B (en) * | 2009-11-17 | 2014-05-14 | 天士力制药集团股份有限公司 | Ginseng glycoside H extract and preparation method thereof |
| CN107752001A (en) * | 2017-10-15 | 2018-03-06 | 长春中医药大学 | A kind of ginseng small molecule oral liquid and preparation method thereof |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN110973432A (en) * | 2019-08-20 | 2020-04-10 | 泰州医药城国科化物生物医药科技有限公司 | Ginseng solid beverage and preparation method thereof |
| CN115260269A (en) * | 2022-06-28 | 2022-11-01 | 成都大学 | Composition containing ginseng glucoside and aglycone thereof, preparation method and application |
| CN115260269B (en) * | 2022-06-28 | 2024-04-05 | 成都大学 | Composition containing ginseng secondary glycoside and aglycone thereof, preparation method and application |
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Application publication date: 20081224 |