CN107652371A - A kind of preparation method of Ganoderma lucidum extract - Google Patents
A kind of preparation method of Ganoderma lucidum extract Download PDFInfo
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- CN107652371A CN107652371A CN201710910838.8A CN201710910838A CN107652371A CN 107652371 A CN107652371 A CN 107652371A CN 201710910838 A CN201710910838 A CN 201710910838A CN 107652371 A CN107652371 A CN 107652371A
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- ganoderma lucidum
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- sintered filter
- pressure
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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Abstract
The invention discloses a kind of preparation method of Ganoderma lucidum extract, it is characterised in that：The device that preparation method uses includes high-pressure pump, heat exchanger, reactor, sintered filter, cooler, sintered filter, minimum pressure valve, the test tube being sequentially connected, and preparation flow is：Pressurized high-temperature processing → impurity screening → extraction → impurity screening → cooling → decompression → collection.Present invention extraction triterpene and β-macromolecule protein polysaccharide are safe and non-toxic, and any adjuvant is not used in production process whole process；Handled and extracted with pressurized hot water, inherently cut off the molecular structure of β-macromolecule protein polysaccharide, realize the extraction of water-soluble beta-macromolecule protein polysaccharide more than 30%；Water is dissolved according to β-macromolecule protein polysaccharide when collecting analyte, the property that triterpene is dissolved in fat carries out lock out operation, realizes that the classification of extract preserves to the full extent.
Description
Technical field
The present invention relates to plant extraction field, particularly a kind of preparation method of Ganoderma lucidum extract.
Background technology
Ganoderma lucidum, because its shape is as deer horn, therefore its name is obtained, in Japan, just formally ganoderma lucidum is entered since the seventies
Row artificial cultivation, 30%~50% can be reached by intending the content of β-macromolecule protein polysaccharide in Ganoderma lucidum, intended Ganoderma lucidum and also contained
There is the LPS for further enhancing immunity by activating macrophage activity.Through examining, LPS+ β-macromolecule egg of dry product
The content of white polysaccharide is up to the 43% of weight.Cellular anti-oxidant capacity can also be improved, regulation moisture of skin suppresses dermal melanin
Formed and precipitated, its dispelling toxicity, detoxicating ability extra-heavy.
It is more on clinical medicine to prepare ganoderma lucidums using concentration effective component of glossy ganoderma and carry in view of the powerful efficacy effect of Ganoderma lucidum
Thing is taken, and is applied to the chemoradiation therapy of tumour, cancer, toxic and side effects of chemoradiotherapy can be mitigated, improves patient to chemicotherapy
Tolerance, enhancing chemicotherapy to the lethal effect of tumour, cancer cell, and reduce the transfer after corrective surgery or chemicotherapy,
Recurrence rate.Wherein exciting, research shows important activity composition --- ganodenic acid and beta glucan in Ganoderma lucidum
It can directly be suppressed by a variety of effective ways or kill tumour, cancer cell.
But the glossy ganoderma extract obtained by general solvent extraction process is used, triterpene and beta glucan content be not high, and
And traditional heating extraction, method for concentration easily cause it to lose activity.Because triterpene substance is liposoluble constituent, and ganoderma lucidum is sub
Liposoluble constituent is seldom in entity, removes outside the insoluble substances such as the chitin for accounting for the dry weight overwhelming majority, cellulose, remaining big portion
Divide the water-soluble substanceses such as carbohydrate, protein, therefore utilize and be adapted to the low temperature CO_2 supercritical technology of liposoluble substance extraction theoretical
On can reach the effect of ganodenic acid in efficient, single-minded enrichment extraction ganoderma lucidum fruitbody.However, often received in practical application
Rate is too low, or collects at all less than product, and reason is that the liposoluble substance in ganoderma lucidum fruitbody is tangible very little, from extraction kettle
In extract after all adhere among device systems pipeline, it is difficult to be collected into final product.Usually using Jia Shui, wine
The method of the artificial entrainer such as essence, ethyl acetate carries out entrainment extraction to ganoderma lucidum fruitbody, above-mentioned to overcome the shortcomings of, but carries
The material of gained is taken to set concentration or drying process useless molten to reduce in addition again containing inedible or even poisonous entrainer
Agent, remove toxic solvent, the method for this reprocessabilty easily cause Ganodenna Lucidum P.E by thermal losses and extend process route institute
Caused by process loss.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preparation method of Ganoderma lucidum extract, its preparation side
Method is changed and the technology that various extractants are extracted is relied on conventional art, ensures depositing without any noxious material in extraction process
On the premise of ensureing not damage extract, caused by by thermal losses and extension process route of Ganodenna Lucidum P.E is being reduced
Process loss.
To reach above-mentioned purpose, technical solution of the invention is：
A kind of preparation method of Ganoderma lucidum extract, it is characterised in that：The device that preparation method uses is included successively
High-pressure pump, heat exchanger, reactor, sintered filter, cooler, sintered filter, minimum pressure valve, the test tube of connection, system
It is for flow：Pressurized high-temperature processing → impurity screening → extraction → impurity screening → cooling → decompression → collection.
Concrete operation step：
(1) pressurized high-temperature is handled：High-pressure pump is added water to, the pressure of whole procedure, then, water are controlled to adjust by high-pressure pump
Heat exchanger is flowed through, the temperature control of whole production procedure is realized using heat exchanger, so as to reach the temperature pressure ring of setting
Border；
(2) impurity screening：Water filters out the impurity in water by sintered filter by sintered filter；Then water enters
Enter reactor；Before water flows into reactor, it is necessary to by sintered filter, the impurity in water is filtered out by filter；
(3) extract：Under the temperature pressure atmosphere of setting, to being placed on the Ganoderma lucidum plant organism of reactor in water
Environment carries out extracting triterpene and β-macromolecule protein polysaccharide；
(4) impurity screening：Reaction product after extraction passes through sintered filter impurity screening；After chemical reaction
Afterwards, meeting doped portion impurity among reactant, utilizes sintered filter to filter unwanted impurity；
(5) cool down：Reaction product after filtering is cooled down through subcooler；Product temperatur after reaction is higher, needs
To be cooled down using cooler；
(6) it is depressured：Reaction stream is through minimum pressure valve；Using the pressure that minimum pressure valve isolation is extraneous, ensure reaction pressure
It is unaffected by force, meanwhile, reduce the pressure values after reaction；
(7) collect：Pressurized hot water analyte is collected with test tube.
Further, the temperature for the temperature pressure atmosphere that (1) is set is 100 DEG C -374 DEG C, pressure 1atm-
217.7atm。
Further, step (3) reactor capacity is 3.5 liters.
Further, high-pressure pump highest pressure voltage is 3MPa among step (1).
Further, described reactor the right and left respectively has a sintered filter.
The remarkable advantage of the present invention is：
1st, extract triterpene using this method and β-macromolecule protein polysaccharide is safe and non-toxic, production process whole process is not used
Any adjuvant；
2nd, handled and extracted with pressurized hot water, inherently cut off the molecular structure of β-macromolecule protein polysaccharide, realize water
The extraction of dissolubility β-macromolecule protein polysaccharide more than 30%；
Water is dissolved according to β-macromolecule protein polysaccharide when the 3rd, collecting analyte, the property that triterpene is dissolved in fat carries out separation behaviour
Make, realize that the classification of extract preserves to the full extent.
Embodiment
With reference to specific embodiment, the invention will be further described.It is herein it should be noted that disclosed below
As long as involved technical characteristic does not form conflict can and is mutually combined each other in each embodiment of the present invention.
A kind of preparation method of Ganoderma lucidum extract, it is characterised in that：The device that preparation method uses is included successively
High-pressure pump, heat exchanger, reactor, sintered filter, cooler, sintered filter, minimum pressure valve, the test tube of connection, system
It is for flow：Pressurized high-temperature processing → impurity screening → extraction → impurity screening → cooling → decompression → collection.
Concrete operation step includes：
(1) pressurized high-temperature is handled：High-pressure pump is added water to, the pressure of whole procedure, pressure model are controlled to adjust by high-pressure pump
Pressure is trapped among between 1atm-217.7atm, high-pressure pump highest pressure voltage is 3MPa, and high-pressure pump is connected with heat exchanger；Secondly,
Water can flow through heat exchanger, set temperature to realize the temperature of whole production procedure using heat exchanger between 100 DEG C -374 DEG C
Control；
(2) impurity screening：Water is filtered out miscellaneous in water by the sintered filter on the left of reactor by sintered filter
Matter；Then water enters reactor；Before water flows into reactor, it is necessary to by sintered filter, water is filtered out by filter
In impurity；
(3) extract：Under the temperature pressure atmosphere set, namely temperature is 100 DEG C -374 DEG C, pressure 1atm-
217.7atm, to be placed on capacity be 3 liters reactor Ganoderma lucidum plant organism water environment carry out extract triterpene and
β-macromolecule protein polysaccharide；
(4) impurity screening：Reaction product after extraction is by the sintered filter impurity screening on the right side of reactor；
After after chemical reaction, meeting doped portion impurity among reactant, sintered filter is utilized to filter unwanted impurity；
(5) reaction product after filtering is cooled down through subcooler；Product temperatur after reaction is higher, it is necessary to utilize
Cooler is cooled down；
(6) it is depressured：Reaction stream is through minimum pressure valve；Using the pressure that minimum pressure valve isolation is extraneous, ensure reaction pressure
It is unaffected by force, meanwhile, reduce the pressure values after reaction；
(7) collect：Pressurized hot water analyte is collected with test tube.
The above described is only a preferred embodiment of the present invention, be not intended to limit the scope of the present invention,
Therefore the change or modification that claim under this invention and specification are done in every case, it should all belong to the scope that patent of the present invention covers
Within.
Claims (6)
- A kind of 1. preparation method of Ganoderma lucidum extract, it is characterised in that：The device that preparation method uses includes connecting successively High-pressure pump, heat exchanger, reactor, sintered filter, cooler, sintered filter, minimum pressure valve, the test tube connect, prepare Flow is：Pressurized high-temperature processing → impurity screening → extraction → impurity screening → cooling → decompression → collection.
- 2. the preparation method of a kind of Ganoderma lucidum extract according to claim 1, it is characterised in that preparation flow includes Operating procedure in detail below：(1) pressurized high-temperature is handled：High-pressure pump is added water to, the pressure of whole procedure is controlled to adjust by high-pressure pump, then, water flows through Heat exchanger, the temperature control of whole production procedure is realized using heat exchanger, so as to reach the temperature pressure atmosphere of setting；(2) impurity screening：Water filters out the impurity in water by sintered filter by sintered filter；Then water enters anti- Answer device；(3) extract：Under the temperature pressure atmosphere of setting, to being placed on the Ganoderma lucidum plant organism of reactor in water environment Carry out extracting triterpene and β-macromolecule protein polysaccharide；(4) impurity screening：Reaction product after extraction passes through sintered filter impurity screening；(5) cool down：Reaction product after filtering is cooled down through subcooler；(6) it is depressured：Reaction stream is through minimum pressure valve；(7) collect：Pressurized hot water analyte is collected with test tube.
- 3. the preparation method of a kind of Ganoderma lucidum extract according to claim 2, it is characterised in that step (1) is set Temperature pressure atmosphere temperature be 100 DEG C -374 DEG C, pressure 1atm-217.7atm.
- 4. the preparation method of a kind of Ganoderma lucidum extract according to claim 2, it is characterised in that step (3) is reacted Device capacity is 3.5 liters.
- 5. the preparation method of a kind of Ganoderma lucidum extract according to claim 2, it is characterised in that among step (1) High-pressure pump highest pressure voltage is 3MPa.
- 6. the preparation method of a kind of Ganoderma lucidum extract according to claim 2, it is characterised in that the reactor is left Respectively there is a sintered filter on right both sides.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710910838.8A CN107652371A (en) | 2017-09-29 | 2017-09-29 | A kind of preparation method of Ganoderma lucidum extract |
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| CN201710910838.8A CN107652371A (en) | 2017-09-29 | 2017-09-29 | A kind of preparation method of Ganoderma lucidum extract |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560072A (en) * | 2004-02-18 | 2005-01-05 | 北京宝泰宁堂生物技术有限公司 | Process of preparing ganoderma tricterpinyl acid and ganoderma polyose from ganoderma |
| CN101357951A (en) * | 2008-09-19 | 2009-02-04 | 南方李锦记有限公司 | Method for extracting ganoderma lucidum fruitbody polysaccharide using ultrahigh pressure |
| CN104223038A (en) * | 2014-08-19 | 2014-12-24 | 上海交通大学 | Preparation method of reishi shell-broken spore powder at temperature difference ultrahigh pressure by wet process |
| CN105294811A (en) * | 2015-09-23 | 2016-02-03 | 成都艾比科生物科技有限公司 | Method for preparing ganoderma lucidum karst triterpenic acid and ganoderma lucidum karst polysaccharide with Nyingchi ganoderma lucidum karst as material |
-
2017
- 2017-09-29 CN CN201710910838.8A patent/CN107652371A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1560072A (en) * | 2004-02-18 | 2005-01-05 | 北京宝泰宁堂生物技术有限公司 | Process of preparing ganoderma tricterpinyl acid and ganoderma polyose from ganoderma |
| CN101357951A (en) * | 2008-09-19 | 2009-02-04 | 南方李锦记有限公司 | Method for extracting ganoderma lucidum fruitbody polysaccharide using ultrahigh pressure |
| CN104223038A (en) * | 2014-08-19 | 2014-12-24 | 上海交通大学 | Preparation method of reishi shell-broken spore powder at temperature difference ultrahigh pressure by wet process |
| CN105294811A (en) * | 2015-09-23 | 2016-02-03 | 成都艾比科生物科技有限公司 | Method for preparing ganoderma lucidum karst triterpenic acid and ganoderma lucidum karst polysaccharide with Nyingchi ganoderma lucidum karst as material |
Non-Patent Citations (1)
| Title |
|---|
| YUI MATSUNAGA等: ""Hot compressed water extraction of polysaccharides from Ganoderma lucidum using a semibatch reactor"", 《ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING》 * |
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