CN102885847B - Uses of camellia oleifera cake polysaccharide - Google Patents
Uses of camellia oleifera cake polysaccharide Download PDFInfo
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- CN102885847B CN102885847B CN201110202761.1A CN201110202761A CN102885847B CN 102885847 B CN102885847 B CN 102885847B CN 201110202761 A CN201110202761 A CN 201110202761A CN 102885847 B CN102885847 B CN 102885847B
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Abstract
The invention relates to novel uses of plant extract and especially relates to novel uses of camellia oleifera cake polysaccharide. The invention provides the use of camellia oleifera cake polysaccharide in inhibition of alpha-glucosidase activity and also provides the use of camellia oleifera cake polysaccharide in preparation of a drug for treating diabetes.
Description
Technical field
The present invention relates to the purposes of plant extract, be specifically related to the purposes of cake of camellia oleifera seeds polysaccharide.
Background technology
Vegetable polysaccharides has many bioactive functions, comprises immunomodulating, antitumor, blood sugar lowering, blood fat reducing, radioprotective, anti-bacteria and anti-virus, the liver protecting etc.Vegetable polysaccharides widely biological activity oneself by people, utilized gradually, the natural sex in its unique activity and source has very large potentiality in ensureing health application.Therefore, how to improve the matter of utmost importance that yield is research vegetable polysaccharides.
At present, China oil tea area approximately has 4,500 ten thousand mu.1,000,000 tons of left and right of Semen Camelliae annual production, the whole nation, 68.39 ten thousand tons of the cake of camellia oleifera seeds after oil expression.
Oil tea side-product comprehensive development and utilization technology mainly concentrates on the development and utilization aspect of tea saponin at present, and the report that other composition develops seldom.Domestic production tea saponin is produced 1.86 ten thousand tons per year at present, has been widely used in daily use chemicals, building materials, medicine and other fields.According to the literature, the content of tea saponin in cake of camellia oleifera seeds is 10～15%, and total sugar content reaches 30～35%, and wherein quite vast scale is polysaccharide.But due to still not fully aware of to the molecular structure of cake of camellia oleifera seeds polysaccharide and function, therefore, oil tea polysaccharide not yet forms industrial scale as tea saponin.
The method that tradition is extracted polysaccharide in cake of camellia oleifera seeds has:
(the food industry such as Wang Yuanfeng, 2008, (6): 29～32) by water extraction, alcohol extracting method being extracted to the optimum extraction condition of camellia seed polysaccharide, tea saponin, compare, determined that the optimum extraction condition of camellia seed polysaccharide, tea saponin makees extraction solvent with water, 75 ℃ of temperature, 2 lixiviates, solid-to-liquid ratio is respectively 1:9 and 1:5, each each 1.5 hours, camellia seed polysaccharide yield 9.09%, mass fraction 20.07%.
(the food industry science and technology such as Wu Xuehui, 2008,29(9): 197～199) adopt microwave technology to extract the polysaccharide in the tea dregs of rice, inquired into the impact of each factor on polysaccharide extraction effect, by orthogonal experiment and extreme difference and linear regression analysis, know that raw material granularity on polysaccharide extraction effect impact in cake of camellia oleifera seeds significantly.Show that best microwave extraction process conditions are: microwave power 640W, extraction time 3min, solid-to-liquid ratio 1: 25, granularity 80～100 orders, extraction time 1 time.Under this condition, the extraction ratio of oil tea polysaccharide is 15.15%.
Huang Baosheng (CN200710191396.2) has invented a kind of method of extracting tea polysaccharide from Semen Camelliae grouts.Specific practice be by camellia seed meal in 10Kg than the ratio of 100-200L water, in water, soak, then heat and stir simultaneously, filter while hot, the liquid after filtering is continued to heating, make liquid concentration to 1/4 volume, the yeast adding in concentrated solution, remain at 25-32 ℃ of temperature, vibration fermentation, after fermentation time 0.5-3 days.Stop vibration, make its precipitation, get the supernatant and add the ethanol of 3 times of clear liquid volumes to stir, then put into refrigerator freezing, freezing liquid is added to water and make it dissolve layering and precipitating, get supernatant liquid, by the spray-dried tea polysaccharide that obtains of supernatant liquid.
Summary of the invention
The purposes that the object of this invention is to provide cake of camellia oleifera seeds polysaccharide.
For achieving the above object, technical scheme of the present invention is:
The invention provides the application of cake of camellia oleifera seeds polysaccharide aspect Inhibiting α-glucosidase activity.The present invention also provides the application of cake of camellia oleifera seeds polysaccharide in preparation treatment diabetes medicament.
Described cake of camellia oleifera seeds polysaccharide is resulting by the extracting method of existing cake of camellia oleifera seeds polysaccharide.
A kind of preferred preparation method of described cake of camellia oleifera seeds polysaccharide, concrete steps are:
(1) cake of camellia oleifera seeds is crushed to 60 order～80 object grouts powder, then grouts powder is added in the aqueous solution that is dissolved with in advance cellulase and extracted; Wherein grouts powder and aqueous solution mass ratio are 1:20～40, and cellulase consumption is 0.3%～0.4% of grouts dried bean noodles weight, and extracting temperature is 45 ℃-55 ℃, 3 hours-5 hours extraction time;
(2) by extracting liquid filtering, adopt hollow fiber ultrafiltration membrane segregation apparatus that filtrate is concentrated, it is 3%～10% that the solution after adjustment is concentrated makes the quality percentage composition of solute in solution, polyamide column absorption 8 hours～12 hours, deionized water eluting;
(3) collect water lotion and concentrate, the ethanol that concentrated solution adds 3～5 times of volumes precipitates 12 hours～36 hours; Centrifugal acquisition precipitate with ethanol precipitation, the dry cake of camellia oleifera seeds polysaccharide that obtains.
The molecular weight of described cake of camellia oleifera seeds polysaccharide is 5253-12210.
The enzyme activity >15000u/g of described cellulase.
Described polysaccharide is canescence polysaccharide.
Above-mentioned preparation method can also be added a step: get above-mentioned thick cake of camellia oleifera seeds polysaccharide appropriate, adopt DEAE-52 column chromatography to carry out classification, use respectively 0.1mol/L NaCl, 0.5mol/L NaCl, 0.5mol/LNaOH eluant solution, collects respectively eluent, concentrating under reduced pressure, flowing water dialysis 24～36 hours, lyophilization, the classification polysaccharide of acquisition different molecular weight.
Below the present invention will be further explained:
Alpha-glucosidase suppresses experiment:
Alpha-glucosidase comprises and is positioned at the α-amylase of small intestinal lumen, the maltase of intestinal brush border, limit dextrinase and saccharase etc.After normal person's feed, carbohydrate in food is if starch is first through α-amylase effect generation maltose, maltotriose, schardinger dextrin etc., by alpha-glucosidase hydrolysis such as saccharases, generate glucose, galactose and fructose subsequently together with sucrose, through intestinal wall Cell uptake, by body, utilized.
Diabetics causes blood sugar concentration generation abnormal change because of dysbolismus.One of effective ways that prevent and treat this class disease are to limit or delay polysaccharide to decompose in digestive tract, just must reduce alpha-glucosidase activity for this reason.Alpha-glucosidase inhibitor is by reversibly occupying alpha-glucosidase and sugared complexation site, and Inhibiting α-glucosidase catalysis polysaccharide is degraded to monosaccharide, thereby delays the absorption of intestinal carbohydrate.Compare with other oral antidiabetic drug and insulinize, the most significant feature of alpha-glucosidase inhibitor medicine is effectively to stop the post-prandial glycemia of diabetics to raise, make patient's blood glucose steadily and lentamente maintain certain level, improve glucostasis index; Owing to having suppressed the decomposition rate of α-glucose glycosidase to intestinal carbohydrate, avoided that contingent blood glucose is too high after the meal.In addition, alpha-glucosidase inhibitor has the effects such as the lipogenesis of adjusting, anti-AIDS-virus infection.Therefore, alpha-glucosidase inhibitor becomes antidiabetic medicine safely and efficiently.
This experiment be take sucrose as substrate, and it can resolve into D-Glucose and D-Fructose under the enzyme catalysis of mucous membrane of small intestine alpha-glucosaccharase, by glucose oxidase method, measures the inhibitory enzyme activity ability that glucose content is analyzed medicine.Application alpha-glucosidase inhibitor high flux screening model screens hypoglycemic drug from compound.Compound is contrasted with contrasting medicine acarbose, the market development of inquiring into compound is worth and as the potentiality of antidiabetic medicine simultaneously.This research is carried out external activity test by obtained cake of camellia oleifera seeds polysaccharide, finds that cake of camellia oleifera seeds polysaccharide has the effect of Inhibiting α-glucosidase activity.
The specific embodiment
Embodiment 1:
(1) preparation of cake of camellia oleifera seeds polysaccharide:
Take cake of camellia oleifera seeds and be crushed to 80 order powder 500g, add water 10000mL, add cellulase (enzyme activity >15000u/g) 1750mg, 50 ℃ of water-baths were extracted after 3 hours, and double gauze filters, and obtains faint yellow extraction solution.Gained extracting solution is concentrated into 1500ml by hollow fiber membrane separation device, the quality percentage composition that makes solute in solution is 6%, by the chromatographic column of prepackage 500g polyamide, fully mix homogeneously, standing adsorption 12 hours, carries out eluting with the deionized water of 4 times of column volumes.Collect the elute soln obtaining, be evaporated to smaller size smaller, add 95% ethanol of 5 times of concentrated solution volumes, fully stir standing 12 hours.Centrifugal (4500 turn/min, 10min).Get the lyophilization of precipitation final vacuum, obtain canescence polysaccharide 9.0g.
(2) test of cake of camellia oleifera seeds polysaccharide vitro inhibition alpha-glucosidase activity:
Embodiment 1 gained cake of camellia oleifera seeds polysaccharide is carried out to the active effect of Inhibiting α-glucosidase, the results are shown in Table 1.
Table 1 cake of camellia oleifera seeds polysaccharide is to the enzyme inhibition of small intestine in rats alpha-glucosaccharase
Embodiment 2:
Get gained polysaccharide 3g in embodiment 1, by the chromatographic column (the high 18cm of post, diameter 3cm, column volume 85cm) of DEAE-52 is housed, with 0.1mol/L NaCl eluant solution, collect eluent, concentrating under reduced pressure, flowing water dialysis 24～36 hours, lyophilization.The classification polysaccharide 129mg(called after CP2 that acquisition relative molecular weight is 8693).The active effect of Inhibiting α-glucosidase, the results are shown in Table 2.
Table 2 CP2 is to the enzyme inhibition of small intestine in rats alpha-glucosaccharase
Embodiment 3:
Get gained polysaccharide 3g in embodiment 1, by chromatographic column (the high 18cm of post of DEAE-52 is housed, diameter 3cm, column volume 85cm), with after 0.1mol/L NaCl eluant solution, continue with 0.5mol/L NaCl eluant solution chromatographic column, collect eluent, concentrating under reduced pressure, flowing water dialysis 24～36 hours, lyophilization.The classification polysaccharide 124mg(called after CP4 that acquisition relative molecular weight is 12210).The active effect of Inhibiting α-glucosidase, the results are shown in Table 3.
Table 3 CP4 is to the enzyme inhibition of small intestine in rats alpha-glucosaccharase
Embodiment 4:
Get gained polysaccharide 3g in embodiment 1, by chromatographic column (the high 18cm of post of DEAE-52 is housed, diameter 3cm, column volume 85cm), after using respectively 0.1mol/L NaCl and 0.5mol/L NaCl eluant solution, continue with 0.5mol/L NaOH eluant solution chromatographic column, collect eluent, concentrating under reduced pressure, flowing water dialysis 24～36 hours, lyophilization.The classification polysaccharide 236mg(called after CP5 that acquisition relative molecular weight is 5253).The active effect of Inhibiting α-glucosidase, the results are shown in Table 4.
Table 4 CP5 is to the enzyme inhibition of small intestine in rats alpha-glucosaccharase
Table 5 contrast medicine acarbose is to the enzyme inhibition of small intestine in rats alpha-glucosaccharase
Claims (2)
1. cake of camellia oleifera seeds polysaccharide is treated the purposes in diabetes medicament in preparation, and the molecular weight of wherein said cake of camellia oleifera seeds polysaccharide is 5253-12210.
2. according to claim, require the purposes described in 1, the cake of camellia oleifera seeds polysaccharide described in it is characterized in that is prepared by following method:
(1) cake of camellia oleifera seeds is crushed to 60 order～80 object grouts powder, then grouts powder is added in the aqueous solution that is dissolved with in advance cellulase and extracted; Wherein grouts powder and aqueous solution mass ratio are 1:20～40, and cellulase consumption is 0.3%～0.4% of grouts dried bean noodles weight, and extracting temperature is 45 ℃-55 ℃, 3 hours-5 hours extraction time;
(2) by extracting liquid filtering, adopt hollow fiber ultrafiltration membrane segregation apparatus that filtrate is concentrated, it is 3%～10% that the solution after adjustment is concentrated makes the quality percentage composition of solute in solution, polyamide column absorption 8 hours～12 hours, deionized water eluting;
(3) collect water lotion and concentrate, the ethanol that concentrated solution adds 3～5 times of volumes precipitates 12 hours～36 hours; Centrifugal acquisition precipitate with ethanol precipitation, the dry cake of camellia oleifera seeds polysaccharide that obtains.
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| CN108003253B (en) * | 2017-12-11 | 2019-05-03 | 盛蓓蓓 | The effect that a kind of Extracted From Oil-tea-cake polysaccharide, preparation method and antagonism aftosa vaccine damage bull semen |
| CN110693897B (en) * | 2019-11-22 | 2023-04-18 | 赣南医学院 | Application of oil tea flesh fruit polysaccharide in preparation of medicine or health-care product for preventing and treating type II diabetes |
| CN113633703A (en) * | 2021-09-16 | 2021-11-12 | 江山之间生物科技有限公司 | Hypoglycemic application of tea extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560265A (en) * | 2009-06-01 | 2009-10-21 | 浙江大学 | Method for preparing oil-tea camellia husk polysaccharide and purifying method |
| CN101942355A (en) * | 2010-08-20 | 2011-01-12 | 上海师范大学 | Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560265A (en) * | 2009-06-01 | 2009-10-21 | 浙江大学 | Method for preparing oil-tea camellia husk polysaccharide and purifying method |
| CN101942355A (en) * | 2010-08-20 | 2011-01-12 | 上海师范大学 | Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds |
Non-Patent Citations (4)
| Title |
|---|
| 从油茶饼粕中提取油茶籽多糖和茶皂素的工艺研究;王元凤 等;《食品工业》;20081231(第06期);29-32 * |
| 周金沙 等.油茶籽的综合利用现状及前景分析.《农产品加工(学刊)》.2006,(第07期),58-60, 78. |
| 油茶籽的综合利用现状及前景分析;周金沙 等;《农产品加工(学刊)》;20060731(第07期);58-60,78 * |
| 王元凤 等.从油茶饼粕中提取油茶籽多糖和茶皂素的工艺研究.《食品工业》.2008,(第06期),29-32. |
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