CN102836194B - Extraction method of affine cudweed total flavonoids - Google Patents
Extraction method of affine cudweed total flavonoids Download PDFInfo
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- CN102836194B CN102836194B CN201110177622.8A CN201110177622A CN102836194B CN 102836194 B CN102836194 B CN 102836194B CN 201110177622 A CN201110177622 A CN 201110177622A CN 102836194 B CN102836194 B CN 102836194B
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- herba gnaphalii
- gnaphalii affinis
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Abstract
The invention discloses an extraction method of affine cudweed total flavonoids, and belongs to the field of medicinal material processing. The extraction method comprises the following steps of: crushing and screening dried affine cudweed; extracting with cellulose by using water as an extracting agent; filtering, and concentrating in reduced pressure, and lyophilizing to obtain the affine cudweed total flavonoid product. The extraction method has the advantages of easy and quick operation, low cost and no toxicity, and is a green production method.
Description
Technical field
The present invention relates to a kind of method of extracting total flavones from Herba Gnaphalii Affinis, specifically a kind of method of utilizing cellulase to extract gnaphalium affine total flavonoid, belongs to field of medicinal materials processing.
Background technology
Herba Gnaphalii Affinis (Gnaphlium affine D.Don) has another name called Herba Gnaphalii Affinis, affine cudweed, is Compositae cottonweed, has the multiple efficacies such as blood fat reducing, blood sugar lowering, blood pressure lowering, defying age, antiinflammatory are antibacterial, enhancing immunity.Before and after the Ching Ming Festival, the tender sharp leaf processing bait of Herba Gnaphalii Affinis that extensively utilizes among the people, therefore there is the title of " affine cudweed ".There are some researches show, in Herba Gnaphalii Affinis, contain more flavone compound.But, both at home and abroad the research of Herba Gnaphalii Affinis flavone compound is also quite lacked at present, and flavone compound has remarkable result to treatment coronary heart disease, alzheimer disease, cerebral thrombosis, nervous system disease and elimination free radical, the aspect such as antibacterial, anticancer, have no side effect, and develop multiple medicine and health food with this.Therefore, how efficiently and safely Herba Gnaphalii Affinis flavone compound to be extracted, be also more and more subject to the attention of Chinese scholars.
Conventional total flavone extracting method has decocting cooking method, and hot reflux is extracted, ultrasound wave assisted extraction, microwave-assisted extraction etc.But decocting cooking method extraction time is very long, general 4～6 hours; Hot reflux is extracted, and ultrasound wave assisted extraction and microwave-assisted extract total flavones to be needed with an organic solvent; And Enzymatic Extraction is as a kind of good extracting method, have simple and efficient to handle, extraction time is short, the rate of transform is high and the feature such as solvent-free pollution, oneself is widely used in the extraction aspect of bioactive substance at present, as Folium Crataegi total flavones, Folium Ginkgo total flavones, Celery etc., and be applied to gnaphalium affine total flavonoid extraction aspect so far there are no report.
Summary of the invention
The extracting method that the object of this invention is to provide a kind of gnaphalium affine total flavonoid, i.e. a kind of method of utilizing cellulase to extract gnaphalium affine total flavonoid.
For achieving the above object, the present invention adopts following technical scheme: dry Herba Gnaphalii Affinis was pulverized to 40 mesh sieves, water is extractant, cellulase consumption is 0.3%～0.5% of Herba Gnaphalii Affinis raw materials quality, the ratio 10ml/g～14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.0～5.0,40 ℃～50 ℃ of hydrolysis temperatures, enzymolysis time 50min～70min, obtains extracting solution, through the check total flavones rate of transform, is greater than 90%; Extracting solution is adopted under vacuum 0.08MPa～0.10MPa and 5 ℃～35 ℃ conditions of temperature Medium speed filter paper filter, obtain filtrate; Filtrate is concentrated under vacuum 0.08MPa～0.10MPa and temperature 50 C～60 ℃ condition initial volume 30% till, obtain concentrated solution; Concentrated solution is less than and dryly under 20Pa, condenser temperature-45 ℃～-60 ℃ of conditions totally obtains gnaphalium affine total flavonoid product in freezer dryer vacuum, through check purity, be greater than 3.5%.
Owing to having adopted above technical scheme, the present invention has the following advantages:
1, the present invention utilizes cellulase to extract gnaphalium affine total flavonoid, its major advantage is to take water as extractant, employing cellulase extracts, extract and compare with alcohol reflux, the extraction of ultrasound wave Assisted Ethanol or Microwave-Assisted Ethanol, during extraction, do not use the organic solvents such as ethanol, so the present invention is easy and simple to handle, quick, cheap, nontoxic;
2, by the inventive method, extract gnaphalium affine total flavonoid, its total flavones rate of transform is greater than 90%, and gnaphalium affine total flavonoid product purity is greater than 3.5%, and favorable reproducibility.
The specific embodiment
Embodiment 1
Dry Herba Gnaphalii Affinis was pulverized 40 mesh sieves, cellulase consumption is 0.40% of Herba Gnaphalii Affinis raw materials quality, the ratio 12ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.5,45 ℃ of hydrolysis temperatures and enzymolysis time 60min, now the total flavones rate of transform is 95.38%; Extracting solution is adopted under vacuum 0.09MPa and 25 ℃ of conditions of temperature Medium speed filter paper filter, obtain filtrate; Filtrate is concentrated under vacuum 0.10MPa and temperature 60 C condition initial volume 30% till, obtain concentrated solution; Concentrated solution is less than under 20Pa, condenser temperature-50 ℃ condition and is dried totally in freezer dryer vacuum, obtain gnaphalium affine total flavonoid product, this product purity is 3.99%.
Embodiment 2
Dry Herba Gnaphalii Affinis was pulverized 40 mesh sieves, cellulase consumption is 0.46% of Herba Gnaphalii Affinis raw materials quality, the ratio 12ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.5,47 ℃ of hydrolysis temperatures and enzymolysis time 60min, now the total flavones rate of transform is 96.92%; Extracting solution is adopted under vacuum 0.09MPa and 25 ℃ of conditions of temperature Medium speed filter paper filter, obtain filtrate; Filtrate is concentrated under vacuum 0.10MPa and temperature 60 C condition initial volume 30% till, obtain concentrated solution; Concentrated solution is less than under 20Pa, condenser temperature-50 ℃ condition and is dried totally in freezer dryer vacuum, obtain gnaphalium affine total flavonoid product, this product purity is 3.86%.
Embodiment 3
Dry Herba Gnaphalii Affinis was pulverized 40 mesh sieves, cellulase consumption is 0.50% of Herba Gnaphalii Affinis raw materials quality, the ratio 14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.8,44 ℃ of hydrolysis temperatures and enzymolysis time 50min, now the total flavones rate of transform is 91.54%; Extracting solution is adopted under vacuum 0.09MPa and 25 ℃ of conditions of temperature Medium speed filter paper filter, obtain filtrate; Filtrate is concentrated under vacuum 0.10MPa and temperature 60 C condition initial volume 30% till, obtain concentrated solution; Concentrated solution is less than under 20Pa, condenser temperature-50 ℃ condition and is dried totally in freezer dryer vacuum, obtain gnaphalium affine total flavonoid product, this product purity is 3.78%.
Claims (1)
1. the extracting method of a gnaphalium affine total flavonoid, it is characterized in that take that Herba Gnaphalii Affinis is as raw material, dry Herba Gnaphalii Affinis was pulverized to 40 mesh sieves, take water as extractant, and adopted cellulase to extract, cellulase consumption is 0.3%～0.5% of Herba Gnaphalii Affinis raw materials quality, ratio 10ml/g～the 14ml/g of the volume of extractant water and Herba Gnaphalii Affinis raw materials quality, pH value 4.0～5.0,40 ℃～50 ℃ of hydrolysis temperatures, enzymolysis time 50min～70min, obtains extracting solution; Again after filtration, concentrating under reduced pressure and lyophilization, obtain gnaphalium affine total flavonoid product;
In described extracting solution, the total flavones rate of transform is greater than 90%;
The method of described filtration is extracting solution to be adopted under vacuum 0.08MPa～0.10MPa and 5 ℃～35 ℃ conditions of temperature Medium speed filter paper filter, and obtains filtrate;
The method of described concentrating under reduced pressure be filtrate is concentrated under vacuum 0.08MPa～0.10MPa and temperature 50 C～60 ℃ condition initial volume 30% till, obtain concentrated solution;
Described cryodesiccated method be by concentrated solution freezer dryer vacuum be less than under 20Pa, condenser temperature-45 ℃～-60 ℃ of conditions dry totally, obtain gnaphalium affine total flavonoid product;
The total flavones product purity of described gnaphalium affine total flavonoid product is greater than 3.5%.
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| CN102836194B true CN102836194B (en) | 2014-04-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103142742B (en) * | 2013-03-21 | 2014-05-07 | 河北康利动物药业有限公司 | Method for preparing oral solution of poplar flower |
| CN103919977A (en) * | 2014-04-29 | 2014-07-16 | 韦江南 | Chinese herbal preparation for treating hyperlipemia |
| CN106858264A (en) * | 2016-12-26 | 2017-06-20 | 福建省龙海市安利达工贸有限公司 | A kind of preparation method of affine cudweed antioxidant |
| CN107094959A (en) * | 2017-06-30 | 2017-08-29 | 邝义槐 | A kind of herbal tea with hypoglycemic effect and preparation method thereof |
| CN108464482A (en) * | 2018-03-05 | 2018-08-31 | 周祥宝 | A kind of preparation method of affine cudweed toppings |
| CN111870649A (en) * | 2020-09-04 | 2020-11-03 | 杨鹏 | Traditional Chinese medicine composition for treating type II diabetes and preparation method thereof |
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| CN1195545A (en) * | 1998-04-14 | 1998-10-14 | 梅景森 | Plaster for curing cough and asthma and its producing method |
| CN101019906A (en) * | 2007-03-19 | 2007-08-22 | 沈信堂 | Chinese medicine for relieving cough, eliminating phlegm and relieving asthma |
| CN101972283A (en) * | 2010-10-19 | 2011-02-16 | 徐州工程学院 | Preparation method of common wedgelet fern herb total flavone |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1195545A (en) * | 1998-04-14 | 1998-10-14 | 梅景森 | Plaster for curing cough and asthma and its producing method |
| CN101019906A (en) * | 2007-03-19 | 2007-08-22 | 沈信堂 | Chinese medicine for relieving cough, eliminating phlegm and relieving asthma |
| CN101972283A (en) * | 2010-10-19 | 2011-02-16 | 徐州工程学院 | Preparation method of common wedgelet fern herb total flavone |
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| 《中药材黄酮类化合物提取分离技术研究进展》;潘俊英 等;《承德医学院学报》;20101231;第3卷(第27期);305-307 * |
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