CN103099827A - Extraction and preparation method of blood-enriching grass leaf flavone - Google Patents
Extraction and preparation method of blood-enriching grass leaf flavone Download PDFInfo
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- CN103099827A CN103099827A CN2013100504422A CN201310050442A CN103099827A CN 103099827 A CN103099827 A CN 103099827A CN 2013100504422 A CN2013100504422 A CN 2013100504422A CN 201310050442 A CN201310050442 A CN 201310050442A CN 103099827 A CN103099827 A CN 103099827A
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- flavone
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- enriching blood
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- 229930003944 flavone Natural products 0.000 title claims abstract description 39
- 235000011949 flavones Nutrition 0.000 title claims abstract description 39
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 30
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 27
- 244000025254 Cannabis sativa Species 0.000 title claims abstract description 25
- 238000000605 extraction Methods 0.000 title claims abstract description 12
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 claims abstract description 11
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 claims abstract description 11
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 claims abstract description 11
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 claims abstract description 10
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 claims abstract description 10
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims abstract description 9
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 7
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 claims abstract description 5
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000005487 catechin Nutrition 0.000 claims abstract description 5
- 229950001002 cianidanol Drugs 0.000 claims abstract description 5
- 229940074391 gallic acid Drugs 0.000 claims abstract description 5
- 235000004515 gallic acid Nutrition 0.000 claims abstract description 5
- 235000008777 kaempferol Nutrition 0.000 claims abstract description 5
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 claims abstract description 5
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims abstract description 4
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims abstract description 4
- PFTAWBLQPZVEMU-UHFFFAOYSA-N catechin Chemical compound OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000005875 quercetin Nutrition 0.000 claims abstract description 4
- 229960001285 quercetin Drugs 0.000 claims abstract description 4
- 239000008280 blood Substances 0.000 claims description 23
- 210000004369 blood Anatomy 0.000 claims description 23
- 239000000843 powder Substances 0.000 claims description 10
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 3
- 230000003252 repetitive effect Effects 0.000 claims description 3
- 206010013786 Dry skin Diseases 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000000287 crude extract Substances 0.000 abstract description 9
- 150000002213 flavones Chemical class 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 7
- 239000000284 extract Substances 0.000 abstract description 5
- 239000002904 solvent Substances 0.000 abstract description 4
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 abstract description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract description 3
- 239000003208 petroleum Substances 0.000 abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 2
- 239000013543 active substance Substances 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000002137 ultrasound extraction Methods 0.000 abstract 1
- 241000201282 Limonium Species 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 3
- -1 Flavone compound Chemical class 0.000 description 3
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 206010008111 Cerebral haemorrhage Diseases 0.000 description 1
- 206010013908 Dysfunctional uterine bleeding Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241000201281 Limonium gmelinii Species 0.000 description 1
- 241001149105 Limonium sinense Species 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
An extraction and preparation method of flavones from leaves of Chinese Buxue grass comprises extracting flavones from wild Buxue grass leaves by ultrasonic extraction method, hydrolyzing to obtain flavone aglycone solution, releasing active substances in crude flavones, and improving health promotion function. Defatting herba Oxalidis Corniculatae leaf with petroleum ether, extracting with ethanol of certain concentration as solvent to obtain herba Oxalidis Corniculatae leaf flavone extract, filtering, concentrating, and drying to obtain herba Oxalidis Corniculatae leaf total flavone crude extract, hydrolyzing the flavone crude extract with hydrochloric acid solution of certain concentration, concentrating, and removing solvent to obtain flavone aglycone solution containing quercetin, kaempferol, catechin and gallic acid.
Description
Technical field
The present invention relates to a kind of extraction hydrolysis process that utilizes the wild blade of grass flavone of enriching blood, belong to Food Science and technical field.
Background technology
China's Herba Limoii Bicoloris is herbaceos perennial, and the lumbaginaceae Statice can be invigorated blood circulation, hemostasis, and warming middle-JIAO and strengthening the spleen, strengthening by means of tonics is used for menoxenia, dysfunctional uterine hemorrhage, bleeding hemorrhoids, gastric ulcer, insufficiency of the spleen edema.The Herba Limonii Gmelinii aboundresources mainly is distributed in the ground such as northeast, the Inner Mongol, Xinjiang, Hebei, Shandong.Therefore, in recent years from limonium sinenseKuntze the abstraction function composition obtained extensive concern.For example, publication number is CN1927237, and application number 200610086169.9 " limonium sinenseKuntze is for the preparation of the purposes of antitumor drug " is to prepare antitumor drug take Herba Limonii Gmelinii as raw material; Publication number is CN101838341A, and application number 201010184071.3 " a kind of method that polysaccharide from limonium sinense kuntze extracts " is to extract polysaccharide take limonium sinenseKuntze as raw material; Publication number is CN101732370A, and application number 200910249049.X " extracts the method for alpha-glucosidase activity inhibitor " from Limonium gmelinii (Wildl.) Kuntze, there is no the report of the blade of grass flavone of enriching blood.
Flavone compound distributes very wide in plant kingdom, be a class secondary metabolism class material that extensively exists in higher plant, the most of and sugared form existence that is combined into glycoside or carbon glycosyl in plant.Medical value is arranged, can reduce the fragility of blood vessel, and improve permeability, reduction blood fat and the cholesterol of blood vessel, can be used for preventing and treating senile hypertension and cerebral hemorrhage.The biological activity of flavone glycoside removing human body oxygen-derived free radicals will be far below flavone aglycone.At present, the exploitation of the blade of grass flavone of enriching blood still belonged to the blank stage.
The present invention adopts solvent that the blade of grass flavone of enriching blood is extracted; and flavone gruff bring up substance is hydrolyzed; obtain flavone aglycone solution; for the deep development utilization of Herba Limonii Gmelinii provides a scientific and effective, is convenient to the preparation technology of large-scale production, also provide new approach for the comprehensive development and utilization of wild Halophyte Resources simultaneously.
Summary of the invention
The object of the invention is to utilize the limonium sinenseKuntze leaf, extract flavone compound, thereby and the hydrolysis flavone extract obtain flavone aglycone, for more wide market prospect is opened up in the industrialization development of Herba Limonii Gmelinii Related product.
Process chart is seen Figure of description, and detailed process is as follows:
(1) the blade of grass drying and crushing of enriching blood
Get the blade of grass of enriching blood clean dry after, 35 ℃ of aeration-dryings are pulverized to constant weight;
(2) enrich blood blade of grass powder preparation of defat
With the blade of grass powder of enriching blood of drying and crushing, the ultrasonic 20min of petroleum ether with 20 times of volumes of its quality carries out desolventizing, and repetitive operation once obtains the defat blade of grass powder of enriching blood;
(3) enrich blood blade of grass crude extract preparation
Take a certain amount of defat blade of grass powder of enriching blood, adopting volume ratio is 70% alcohol solvent supersound extraction 40min, solid-liquid ratio 1:40.After extraction is completed, filter and to discard precipitation, precipitation repeats to extract once, merge extractive liquid,, and under lower than the condition of 60 ℃ vacuum-concentrcted, desolventizing.Then it is carried out obtaining powdery total flavones crude extract after vacuum lyophilization;
(4) enrich blood blade of grass flavone aglycone preparation
Take a certain amount of total flavones crude extract, with 4.0 M HC1 of 80% ethanol and 40 times of volumes of 80 times of volumes of its quality, 70 ℃ of hydrolysis 4h, concentrating under reduced pressure desolventizing and volatile matter obtain flavone aglycone solution;
(5) product the key technical indexes
In the total flavones crude extract, general flavone content is 700-1200 mg/100g, contains Quercetin in flavone aglycone solution, kaempferol, catechin and gallic acid;
(6) detection method
Adopt alkaline NaNO
2-Al (NO
3)
3Development process in wavelength 510nm place measuring samples light absorption value, calculates Herba Limonii Gmelinii leaf flavonoids content according to standard curve take rutin as standard substance.Adopt high effective liquid chromatography for measuring Quercetin, kaempferol, catechin and gallic acid content.
The specific embodiment
The present invention is described in further detail by following examples, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
The blade of grass of enriching blood of drying is ground into powder, with the petroleum ether supersound extraction 20min defat of 20 times of volumes of its quality, the blade of grass powder of enriching blood after defat is carried out desolventizing, repetitive operation once obtains the defat blade of grass powder of enriching blood.The defat that the takes a certain amount of drying blade of grass powder of enriching blood adds the 70%(volume ratio) alcoholic solution, supersound extraction 40min, the ethanol consumption is solid-liquid ratio 1:40, filters after taking into, repeats once, collects merging filtrate.With the centrifugal remove impurity of above-mentioned Herba Limonii Gmelinii extracting solution, collect supernatant stand-by.Supernatant is carried out vacuum-concentrcted, and thickening temperature is higher than 60 ℃, vacuum-0.1～-0.06MPa, desolventizing.Then it is carried out obtaining powdery total flavones crude extract after vacuum lyophilization.With 4.0 M HC1 of 80% ethanol and 40 times of volumes of 80 times of volumes of powdery total flavones crude extract quality, 70 ℃ of hydrolysis 4h, concentrating under reduced pressure desolventizing and volatile matter obtain flavone aglycone solution.In the total flavones crude extract, general flavone content is 1132mg/100g, quercetin content 1.11 mg/100g in flavone aglycone solution, kaempferol content 0.16 mg/100g, catechin content 122 mg/100g, gallic acid content 157 mg/100g.
Claims (1)
1. the extraction preparation method of the blade of grass flavone of enriching blood is characterized in that comprising following preparation process:
(1) get the wild blade of grass of enriching blood and clean and to dry, pulverize to constant weight 35 ℃ of lower aeration-dryings, defat obtains the defat blade of grass powder of enriching blood;
(2) with 70% ethanol (volume ratio) supersound extraction step (1) the blade of grass powder of enriching blood, the extraction Alcohol soluble composition in blade of grass of enriching blood, obtain extracting solution, extraction time is 40min, solid-liquid ratio is 1:40, repetitive operation once, merge extractive liquid,, reclaim ethanol, obtain the powdery flavone gruff bring up substance through after vacuum lyophilization;
(3) flavone gruff bring up substance that obtains of step (2) adds 80% ethanol and 4.0 M HC1,70 ℃ of hydrolysis 4h, and concentrating under reduced pressure desolventizing and volatile material obtain flavone aglycone solution, wherein contain Quercetin, kaempferol, catechin and gallic acid.
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| CN2013100504422A CN103099827A (en) | 2013-02-13 | 2013-02-13 | Extraction and preparation method of blood-enriching grass leaf flavone |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100504422A CN103099827A (en) | 2013-02-13 | 2013-02-13 | Extraction and preparation method of blood-enriching grass leaf flavone |
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| CN103099827A true CN103099827A (en) | 2013-05-15 |
Family
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494847A (en) * | 2013-09-29 | 2014-01-08 | 西北师范大学 | Method for extracting yellow pigment from limonium aureum |
| CN105132172A (en) * | 2015-08-28 | 2015-12-09 | 安徽中烟工业有限责任公司 | Method for preparing orris root flavonoid matter for tobaccos from orris roots |
-
2013
- 2013-02-13 CN CN2013100504422A patent/CN103099827A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 中国医学科学院药物研究所编: "《中草药有效成分的研究（第一分册）提取、分离、鉴定和含量测定》", 30 September 1972, 人民卫生出版社 * |
| 冉晓燕: "槐角中总黄酮分离提取工艺、山奈素纯化工艺及生物活性研究", 《贵州大学硕士学位论文》 * |
| 张博雅: "槐角异黄酮的提取、纯化分离、水解及清除自由基能力的研究", 《东北农业大学硕士学位论文》 * |
| 李晓雁、甄润英 等: "二色补血草黄酮的提取工艺及抗氧化功能研究", 《安徽农业科学》 * |
| 谷建: "均匀设计法优化超声提取黄花补血草中总黄酮", 《中药材》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494847A (en) * | 2013-09-29 | 2014-01-08 | 西北师范大学 | Method for extracting yellow pigment from limonium aureum |
| CN105132172A (en) * | 2015-08-28 | 2015-12-09 | 安徽中烟工业有限责任公司 | Method for preparing orris root flavonoid matter for tobaccos from orris roots |
| CN105132172B (en) * | 2015-08-28 | 2019-06-18 | 安徽中烟工业有限责任公司 | A method of preparing tobacco orrisroot Flavonoid substances from orrisroot |
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Application publication date: 20130515 |