CN105168326A - Radix puerariae extract preparation method - Google Patents
Radix puerariae extract preparation method Download PDFInfo
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- CN105168326A CN105168326A CN201510357381.3A CN201510357381A CN105168326A CN 105168326 A CN105168326 A CN 105168326A CN 201510357381 A CN201510357381 A CN 201510357381A CN 105168326 A CN105168326 A CN 105168326A
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Abstract
The present invention discloses a radix puerariae extract preparation method, which comprises: a) taking washed radix puerariae, grinding to obtain a slurry, adding cellulase to the radix puerariae slurry, drying the enzymolysis mixing liquid, and crushing; b) adding to ethanol, and carrying out microwave-assisted extraction; c) repeatedly extracting the filtration residue three times; d) merging the filtrates, recovering the ethanol, and concentrating the residue liquid; and e) carrying out static adsorption with a macroporous resin column, eluting with ethanol, and carrying out spray-drying to obtain the radix puerariae extract. According to the present invention, on the basis of the ethanol reflux extraction, the microwave-assisted extraction is used, such that the penetrating power is strong, the operating time is short, and the mass fraction of the active ingredient is substantially improved; the resin adsorption purification is used and the resin can be regenerated and recycled, such that the industrial continuous production can be achieved, and the production cost is saved; and the enzymolysis cellulose is used to extract a lot of the puerarin in the crude fibers of the radix puerariae epidermis, the process is simple, the extraction is efficient, the extraction operation and the final product are non-toxic and harmless, and the pollution on the environment is reduced.
Description
[technical field]
The present invention relates to the technical field of health food, particularly a kind of technical field of preparation method of Radix Puerariae extract.
[background technology]
Radix Puerariae, Chinese medicine name.For the dry root of legume pueraria lobata, practise and claim Herba Gelsemii Elegantis.Dry.Sweet, pungent, cool.There is expelling pathogenic factors from muscles for reducing heat, rash, promoting the production of body fluid to quench thirst, the merit of yang invigorating antidiarrheal.Mainly contain relieve the effect of alcohol, blood fat reducing, blood pressure lowering, pathogenic fire reducing, heat-clearing and toxic substances removing, yang invigorating expelling pathogenic factors from muscles, spasmolysis and analgesia, rash antidiarrheal, increase brain and crown blood glucose, improve the effects such as brain microcirculation, be the important source material of the medicine preventing and treating the various diseases such as hypertension, hyperlipidemia, coronary heart diseases and angina pectoris, diabetes, cerebral thrombosis, gastroenteropathy, to women's cosmetology and health etc., there is special efficacy simultaneously.
Radix Puerariae is traditional Chinese crude drug, is again superior health food.In China, people eat the long history having 3,000 years with medicinal Radix Puerariae at least.Said after Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) tastes Radix Puerariae: " Radix Puerariae; people all steamed wheaten foods it; ... fleshiness and few muscle; sweet and refreshing ... " show according to modern pharmacological research, Radix Puerariae contains multiple composition of curing the disease, such as Flavonoid substances (Gly, Acetylpuerarin, arachidic acid etc.), therefore, it have antipyreticly to promote the production of body fluid, invigorating YANG QI to promote eruption, the effects such as relieving muscles diaphoresis, the diseases such as the headache of every diseases caused by exogenous pathogenic factor fever, nape aching, measles initial stage fever, chronic diarrhea, calentura are thirsty, hypertension, angina pectoris, migraine, all can treat with Radix Puerariae.Radix Puerariae extract derives from the dry root of plant Herba Gelsemii Elegantis, and active component is isoflavone (puerarin, daidzin, daidzein, genistein).
Existing Radix Puerariae volume of production is sufficient but due to its extracting method imperfection, production efficiency is low, and energy consumption is large, does not have competitiveness to making product.Traditional method uses solvent extraction method, and general is dissolved as methanol, ethanol etc., and this method rate of output is low, and product purity is lower than 50%, and a series of shortcoming such as the time is very long.A large amount of Radix Puerariae flavones because of be present in Radix Puerariae epidermis crude fibre in cannot be extracted, extract of poor benefits, be not suitable for large-scale production.
[summary of the invention]
Object of the present invention solves the problems of the prior art exactly, a kind of preparation method of Radix Puerariae extract is proposed, by on the basis of alcohol reflux, use microwave radiation exaraction, penetration power is strong, operating time is short, effective ingredient mass fraction improves greatly, and finally adopt resin absorption purification, resin is renewable to be reused, be applicable to industrial continuous seepage, save production cost.Using enzymolysis cellulose, also extracting being present in Radix Puerariae flavones a large amount of in the crude fibre of Radix Puerariae epidermis, technique is simple, extracts efficient, extract operation and end product harmless nontoxic, reduce the pollution to environment.
For achieving the above object, the present invention proposes a kind of preparation method of Radix Puerariae extract, comprise concrete steps as follows:
A) get the Radix Puerariae of cleaning, send into fiberizer, add water mixing defibrination, obtain Radix Puerariae serosity, in Radix Puerariae serosity, add cellulase, carry out enzymolysis, enzymolysis mixed liquor is dried, after pulverizing with pulverizer, cross 40 mesh sieve, the Radix Puerariae process powder obtained;
B) the Radix Puerariae process powder obtained adds in 60% ethanol, then put in extractor carry out first time extract, microwave radiation exaraction, cold filtration, obtains Radix Puerariae extracting solution and filtering residue;
C) then by filtering the filtering residue obtained for the first time, adding ethanol, reacting with under b) identical condition, repeating extraction three times;
The filtrate of d) extracting four times merges, and ethanol is reclaimed in first distilling under reduced pressure, to residual liquid without alcohol taste, then is concentrated by residual liquid;
When e) being concentrated into 1:2, use macroporous resin column static adsorption, distilled water eluting, then the ethanol elution using 60% ~ 70%, collect eluent, spraying dry, obtains Radix Puerariae extract.
As preferably, described step a) in Radix Puerariae select Radix puerariae, trophophase is 3 ~ 5 years.
As preferably, described step a) in, the amount adding cellulase is Radix Puerariae quality 0.2% ~ 0.5%, and enzymolysis time is 1 ~ 3 hour, and the temperature of enzymolysis process is 35 DEG C ~ 45 DEG C.
As preferably, described step b) and c) in, it is 1g:10 ~ 20ml that first time extracts powder of Radix Puerariae with the solid-to-liquid ratio of ethanol, and second time is extracted and third time extraction, and the solid-to-liquid ratio of powder of Radix Puerariae and ethanol is 1g:6 ~ 16ml; Ethanol used is edible ethanol.
As preferably, described step b) in, adopt the microwave action time to be 20 ~ 40s, microwave intensity is medium-to-high grade.
As preferably, described step b) in, carry out first time in extractor and extract and be heated to 30 ~ 60 DEG C, ethanol is 40 ~ 80 minutes to the extraction time of powder of Radix Puerariae.
As preferably, described step e) in, macroporous resin column selects S-8 resin column static adsorption, and concentrated liquid measure is 3 times of resin column volumes, and ethanol consumption is 5 times of resin column volumes, and flow velocity is 1.5mL/min.
Beneficial effect of the present invention: the present invention is by the basis of alcohol reflux, use microwave radiation exaraction, penetration power is strong, operating time is short, effective ingredient mass fraction improves greatly, and finally adopt resin absorption purification, resin is renewable to be reused, be applicable to industrial continuous seepage, save production cost.Using enzymolysis cellulose, also extracting being present in Radix Puerariae flavones a large amount of in the crude fibre of Radix Puerariae epidermis, technique is simple, extracts efficient, extract operation and end product harmless nontoxic, reduce the pollution to environment.
[detailed description of the invention]
The preparation method of a kind of Radix Puerariae extract of the present invention, comprises concrete steps as follows:
A) get the Radix Puerariae of cleaning, send into fiberizer, add water mixing defibrination, obtain Radix Puerariae serosity, in Radix Puerariae serosity, add cellulase, carry out enzymolysis, enzymolysis mixed liquor is dried, after pulverizing with pulverizer, cross 40 mesh sieve, the Radix Puerariae process powder obtained;
B) the Radix Puerariae process powder obtained adds in 60% ethanol, then put in extractor carry out first time extract, microwave radiation exaraction, cold filtration, obtains Radix Puerariae extracting solution and filtering residue;
C) then by filtering the filtering residue obtained for the first time, adding ethanol, reacting with under b) identical condition, repeating extraction three times;
The filtrate of d) extracting four times merges, and ethanol is reclaimed in first distilling under reduced pressure, to residual liquid without alcohol taste, then is concentrated by residual liquid;
When e) being concentrated into 1:2, use macroporous resin column static adsorption, distilled water eluting, then the ethanol elution using 60% ~ 70%, collect eluent, spraying dry, obtains Radix Puerariae extract.
Described step a) middle Radix Puerariae selects Radix puerariae, and trophophase is 3 ~ 5 years.Described step a) in, the amount adding cellulase is Radix Puerariae quality 0.2% ~ 0.5%, and enzymolysis time is 1 ~ 3 hour, and the temperature of enzymolysis process is 35 DEG C ~ 45 DEG C.Described step b) and c) in, the solid-to-liquid ratio that first time extracts powder of Radix Puerariae and ethanol is 1g:10 ~ 20ml, and second time is extracted and third time extraction, and the solid-to-liquid ratio of powder of Radix Puerariae and ethanol is 1g:6 ~ 16ml; Ethanol used is edible ethanol.Described step b) in, adopt the microwave action time to be 20 ~ 40s, microwave intensity is medium-to-high grade.Described step b) in, carry out first time extraction in extractor and be heated to 30 ~ 60 DEG C, ethanol is 40 ~ 80 minutes to the extraction time of powder of Radix Puerariae.Described step e) in, macroporous resin column selects S-8 resin column static adsorption, and concentrated liquid measure is 3 times of resin column volumes, and ethanol consumption is 5 times of resin column volumes, and flow velocity is 1.5mL/min.
Embodiment
A) Radix puerariae that trophophase is 3 years is got, cleaning Radix Puerariae, send into fiberizer, add water mixing defibrination, obtain Radix Puerariae serosity, in Radix Puerariae serosity, add cellulase, the amount adding cellulase is Radix Puerariae quality 0.5%, carry out enzymolysis, the temperature of enzymolysis process is 40 DEG C, and enzymolysis time is 1.5 hours.Enzymolysis mixed liquor is dried, after pulverizing with pulverizer, crosses 40 mesh sieve, the Radix Puerariae process powder obtained;
B) the Radix Puerariae process powder obtained adds in 60% ethanol, put into again in extractor and carry out first time extraction, the solid-to-liquid ratio that first time extracts powder of Radix Puerariae and ethanol is 1g:12ml, carry out first time extraction in extractor and be heated to 40 DEG C, extraction time is 60 minutes, microwave radiation exaraction, the microwave action time is adopted to be 30s, microwave intensity is medium-to-high grade, and cold filtration obtains Radix Puerariae extracting solution and filtering residue;
C) then by filtering the filtering residue obtained for the first time, adding ethanol, reacting with under b) identical condition, repeating extraction three times; Second time is extracted and third time extracts, and the solid-to-liquid ratio of powder of Radix Puerariae and ethanol is 1g:10ml;
The filtrate of d) extracting four times merges, and ethanol is reclaimed in first distilling under reduced pressure, to residual liquid without alcohol taste, then is concentrated by residual liquid;
When e) being concentrated into 1:2, use macroporous resin column static adsorption, macroporous resin column selects S-8 resin column static adsorption, and concentrated liquid measure is 3 times of resin column volumes, and flow velocity is 1.5mL/min.Distilled water eluting, then the ethanol elution using 60%, ethanol consumption is 5 times of resin column volumes, and collect eluent, spraying dry, obtains Radix Puerariae extract.
The present invention, by the basis of alcohol reflux, use microwave radiation exaraction, penetration power is strong, operating time is short, and effective ingredient mass fraction improves greatly, finally adopts resin absorption purification, resin is renewable to be reused, and is applicable to industrial continuous seepage, saves production cost.Using enzymolysis cellulose, also extracting being present in Radix Puerariae flavones a large amount of in the crude fibre of Radix Puerariae epidermis, technique is simple, extracts efficient, extract operation and end product harmless nontoxic, reduce the pollution to environment.
Above-described embodiment is to explanation of the present invention, is not limitation of the invention, anyly all belongs to protection scope of the present invention to the scheme after simple transformation of the present invention.
Claims (7)
1. a preparation method for Radix Puerariae extract, is characterized in that: comprise concrete steps as follows:
A) get the Radix Puerariae of cleaning, send into fiberizer, add water mixing defibrination, obtain Radix Puerariae serosity, in Radix Puerariae serosity, add cellulase, carry out enzymolysis, enzymolysis mixed liquor is dried, after pulverizing with pulverizer, cross 40 mesh sieve, the Radix Puerariae process powder obtained;
B) the Radix Puerariae process powder obtained adds in 60% ethanol, then put in extractor carry out first time extract, microwave radiation exaraction, cold filtration, obtains Radix Puerariae extracting solution and filtering residue;
C) then by filtering the filtering residue obtained for the first time, adding ethanol, reacting with under b) identical condition, repeating extraction three times;
The filtrate of d) extracting four times merges, and ethanol is reclaimed in first distilling under reduced pressure, to residual liquid without alcohol taste, then is concentrated by residual liquid;
When e) being concentrated into 1:2, use macroporous resin column static adsorption, distilled water eluting, then the ethanol elution using 60% ~ 70%, collect eluent, spraying dry, obtains Radix Puerariae extract.
2. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, is characterized in that: described step a) middle Radix Puerariae selects Radix puerariae, and trophophase is 3 ~ 5 years.
3. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, is characterized in that: described step a) in, the amount adding cellulase is Radix Puerariae quality 0.2% ~ 0.5%, and enzymolysis time is 1 ~ 3 hour, and the temperature of enzymolysis process is 35 DEG C ~ 45 DEG C.
4. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, it is characterized in that: described step b) and c) in, the solid-to-liquid ratio that first time extracts powder of Radix Puerariae and ethanol is 1g:10 ~ 20ml, second time is extracted and third time extracts, and the solid-to-liquid ratio of powder of Radix Puerariae and ethanol is 1g:6 ~ 16ml; Ethanol used is edible ethanol.
5. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, is characterized in that: described step b) in, adopt the microwave action time to be 20 ~ 40s, microwave intensity is medium-to-high grade.
6. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, is characterized in that: described step b) in, carry out first time extraction in extractor and be heated to 30 ~ 60 DEG C, ethanol is 40 ~ 80 minutes to the extraction time of powder of Radix Puerariae.
7. the preparation method of a kind of Radix Puerariae extract as claimed in claim 1, it is characterized in that: described step e) in, macroporous resin column selects S-8 resin column static adsorption, and concentrated liquid measure is 3 times of resin column volumes, ethanol consumption is 5 times of resin column volumes, and flow velocity is 1.5mL/min.
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Cited By (4)
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| CN106419364A (en) * | 2016-08-24 | 2017-02-22 | 十堰市绿田生物科技有限公司 | Cervical vertebra shaped medicinal pillow containing radix puerariae and manufacturing method of medicinal pillow |
| CN107156834A (en) * | 2016-03-08 | 2017-09-15 | 云南奥咖生物技术有限公司 | A kind of production technology of Maca extract |
| CN108685061A (en) * | 2017-04-12 | 2018-10-23 | 西南大学 | A kind of pueraria lobata ultramicro whole powder and the composition comprising it and application |
| CN114533631A (en) * | 2022-03-18 | 2022-05-27 | 广西农业职业技术大学 | Kudzu vine root and dendrobium whitening cream and preparation method thereof |
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| CN101766671A (en) * | 2008-12-30 | 2010-07-07 | 郑鹏 | Pharmaceutical composition for preventing and treating restenosis after coronary stent implantation and preparation method thereof |
| CN101736052A (en) * | 2009-12-25 | 2010-06-16 | 重庆正里元实业有限公司 | Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107156834A (en) * | 2016-03-08 | 2017-09-15 | 云南奥咖生物技术有限公司 | A kind of production technology of Maca extract |
| CN106419364A (en) * | 2016-08-24 | 2017-02-22 | 十堰市绿田生物科技有限公司 | Cervical vertebra shaped medicinal pillow containing radix puerariae and manufacturing method of medicinal pillow |
| CN108685061A (en) * | 2017-04-12 | 2018-10-23 | 西南大学 | A kind of pueraria lobata ultramicro whole powder and the composition comprising it and application |
| CN114533631A (en) * | 2022-03-18 | 2022-05-27 | 广西农业职业技术大学 | Kudzu vine root and dendrobium whitening cream and preparation method thereof |
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