CN110251596A - A kind of extracting method of safflower active constituent - Google Patents
A kind of extracting method of safflower active constituent Download PDFInfo
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- CN110251596A CN110251596A CN201910491529.0A CN201910491529A CN110251596A CN 110251596 A CN110251596 A CN 110251596A CN 201910491529 A CN201910491529 A CN 201910491529A CN 110251596 A CN110251596 A CN 110251596A
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- safflower
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract
The present invention relates to a kind of extracting methods of safflower active constituent, it is main by cleaning, it is dry, broken, ultrasonic just mention, ultrasonic essence the methods of mentions, is centrifuged, be concentrated obtained crocus sativus, the extracting solution can promote scalp blood circulation, hair growing and other effects is played, while safflower extraction process of the invention is simply easily operated, recovery rate is higher.
Description
Technical field
The invention belongs to plant extracts fields, and in particular to a kind of extracting method of safflower active constituent.
Background technique
Since ancient times, safflower is just used as medicinal material, fragrance or dyestuff to be used widely between east and west, can be used for developing function
The fields such as energy property food, natural pigment, health cosmetics.But safflower is mainly that column cap is used as medicine, and low output, resource is limited,
Expensive, be otherwise known as " plant gold ".The principle active component of safranine flower extract is saffron glucoside constituents, modern medicine
Reason is studies have shown that the extract of safflower has the effects of anti-oxidant, activating microcirculation and removing stasis medicinal, liver-protecting and cancer-resisting.Utilize safranine flower extract
The effect of the effect of activating microcirculation and removing stasis medicinal, may be added to that hair washing strengthen the hair product, reach strengthen the hair hair tonic by promotion scalp blood circulation.
The extractive technique of traditional safflower has water extraction and lye to extract two kinds of extracting methods, but both extracting methods are all
There is some problems, though water extraction recovery rate easy to operate is lower；And lye extracts the effective component that can destroy safflower.
Summary of the invention
The present invention provides a kind of extracting methods of safflower active constituent to be adopted by the way that ethyl alcohol is added as Extraction solvent
Crocus sativus is extracted with Ultrasonic Heating method.The safflower extraction process of the invention is simply easily operated, recovery rate is higher.
Specifically, extracting method of the present invention is as follows:
A kind of extracting method of safflower active constituent, it is characterised in that safflower is added ethyl alcohol and makees after break process
For solvent, ultrasonic extraction is obtained.
A kind of extracting method of safflower active constituent, which comprises the following steps:
Step 1: safflower being placed in 45 DEG C of baking ovens and dries 10-12h, crosses 40-60 mesh after being crushed with high speed disintegrator
Sieve obtains saffron crocus powder；
Step 2: the saffron crocus powder of step 1 is placed in a beaker, and is 1: 30-50 addition matter according to feed liquid mass ratio
The ethanol solution ultrasonic extraction that score is 40%-70%% is measured, wherein ultrasonic temperature is 40-60 DEG C, extraction time 40-
60min filters off melt cinder, obtains extracting solution 1；
Step 3: being added the 40%-70% ethanol solution ultrasonic extraction of step 2) moderate in the melt cinder in step 2,
Melt cinder is filtered off, wherein ultrasonic temperature is 40-60 DEG C, and extraction time 40-60min obtains extracting solution 2, by extracting solution 1 and is extracted
Liquid 2 mixes；
Step 4: extracting solution mixed in step 3 is subjected to high speed refrigerated centrifuge, to obtain the higher safranine of purity
Flower extracting solution, wherein high speed freezing centrifuging temperature is 4 DEG C, centrifugation time 5-10min, revolving speed 9000rpm；
Step 5: the extracting solution in step 4 being concentrated using Rotary Evaporators, makes extracting solution quality after concentration
10 times of initial safflower quality, to obtain the higher crocus sativus of concentration, wherein thickening temperature is 40-60 DEG C；
Step 6: being added a certain amount of bacteriostatic agent in the extracting solution in step 5, wherein bright string coccus/radish root hair
Ferment Product filtrate, additional amount 2-3%.
Detailed description of the invention
Fig. 1 is that technique extracts flow chart.
Beneficial effects of the present invention
Since safflower is rare medicinal material, pharmacological activity main component is safflower glycoside, therefore saffron glucoside
Recovery rate is main target, and EtOH Sonicate of the present invention extracts saffron glucoside, and recovery rate is about 30%, and recovery rate is higher.
Specific embodiment
The present invention is described in further detail by the following examples, but protection scope of the present invention is not by specific reality
Any restrictions of example are applied, but are defined in the claims.
Embodiment 1
The present invention provides a kind of extracting methods of safflower active constituent, comprising the following steps:
Step 1: safflower being placed in 45 DEG C of baking ovens and dries 12h, is crossed 40 meshes after being crushed with high speed disintegrator and is obtained
Saffron crocus powder；
Step 2: saffron crocus powder is placed in a beaker, according to feed liquid mass ratio be 1: 30 addition mass fraction be 60%
Ethanol solution ultrasonic extraction, wherein ultrasonic temperature be 40 DEG C, extraction time 60min, filter off melt cinder, obtain extracting solution 1；
Step 3: 60% ethanol solution ultrasonic extraction of step 2 moderate, filter are added in the melt cinder in step 2
Melt cinder is removed, wherein ultrasonic temperature is 40 DEG C, and extraction time 60min obtains extracting solution 2, and extracting solution 1 and extracting solution 2 are mixed；
Step 4: extracting solution mixed in step 3 is subjected to high speed refrigerated centrifuge, to obtain the higher safranine of purity
Flower extracting solution, wherein high speed freezing centrifuging temperature is 4 DEG C, centrifugation time 10min, revolving speed 9000rpm；
Step 5: the extracting solution in step 4 being concentrated using Rotary Evaporators, makes extracting solution quality after concentration
10 times of initial safflower quality, wherein thickening temperature is 40 DEG C；
Step 6: being added bright string coccus/radish root tunning filtrate in the extracting solution in step 5, additional amount is
2%.
Embodiment 2
The present invention provides a kind of extracting methods of safflower active constituent, comprising the following steps:
Step 1: safflower being placed in 45 DEG C of baking ovens and dries 12h, is crossed 40 meshes after being crushed with high speed disintegrator and is obtained
Saffron crocus powder；
Step 2: saffron crocus powder is placed in a beaker, according to feed liquid mass ratio be 1: 50 addition mass fraction be 50%
Ethanol solution ultrasonic extraction, wherein ultrasonic temperature be 50 DEG C, extraction time 60min, filter off melt cinder, obtain extracting solution 1；
Step 3: being added 50% ethanol solution ultrasonic extraction of step 2 moderate in the melt cinder in step 2, filters off
Melt cinder, wherein ultrasonic temperature is 50 DEG C, and extraction time 60min obtains extracting solution 2, and extracting solution 1 and extracting solution 2 are mixed；
Step 4: extracting solution mixed in step 3 is subjected to high speed refrigerated centrifuge, to obtain the higher safranine of purity
Flower extracting solution, wherein high speed freezing centrifuging temperature is 4 DEG C, centrifugation time 5min, revolving speed 9000rpm；
Step 5: the extracting solution in step 4 being concentrated using Rotary Evaporators, makes extracting solution quality after concentration
10 times of initial safflower quality, wherein thickening temperature is 50 DEG C；
Step 6: being added bright string coccus/radish root tunning filtrate in the extracting solution in step 5, additional amount is
2.5%.
Embodiment 3
The present invention provides a kind of extracting methods of safflower active constituent, comprising the following steps:
Step 1: safflower being placed in 45 DEG C of baking ovens and dries 10h, is crossed 60 meshes after being crushed with high speed disintegrator and is obtained
Saffron crocus powder；
Step 2: saffron crocus powder is placed in a beaker, according to feed liquid mass ratio be 1: 40 addition mass fraction be 70%
Ethanol solution ultrasonic extraction, wherein ultrasonic temperature be 60 DEG C, extraction time 60min, filter off melt cinder, obtain extracting solution 1；
Step 3: being added 70% ethanol solution ultrasonic extraction of step 2 moderate in the melt cinder in step 3, filters off
Melt cinder, wherein ultrasonic temperature is 60 DEG C, and extraction time 60min obtains extracting solution 2, and extracting solution 1 and extracting solution 2 are mixed；
Step 4: extracting solution mixed in step 3 is subjected to high speed refrigerated centrifuge, to obtain the higher safranine of purity
Flower extracting solution, wherein high speed freezing centrifuging temperature is 4 DEG C, centrifugation time 10min, revolving speed 9000rpm；
Step 5: the extracting solution in step 4 being concentrated using Rotary Evaporators, makes extracting solution quality after concentration
10 times of initial safflower quality, wherein thickening temperature is 60 DEG C；
Step 6: being added bright string coccus/radish root tunning filtrate in the extracting solution in step 5, additional amount is
3%.
Following table is saffron glucoside recovery rate in different embodiments
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Saffron glucoside recovery rate (%) | 30.17 | 28.36 | 29.68 |
As seen from the above embodiment, the recovery rate of saffron glucoside is about 30% in extracting method of the present invention, is extracted
Rate is higher, and furthermore the extracting method simple process, easy to operate, is a kind of preferable extracting method.
Claims (10)
1. a kind of extracting method of safflower active constituent, it is characterised in that: ethyl alcohol conduct is added after break process in safflower
Solvent, ultrasonic extraction.
2. a kind of extracting method of safflower active constituent, which comprises the following steps:
1) it extracts pre-treatment: safflower being placed in 45 DEG C of baking ovens and is dried, after being crushed with high speed disintegrator, sieving obtains safflower
Powder；
2) it just mentions: the saffron crocus powder of step 1) is placed in a beaker, ethanol solution ultrasonic extraction is added, filter off melt cinder, obtain
Extracting solution 1；
3) essence mentions: the ethanol solution ultrasonic extraction of step 2) moderate being added in the melt cinder in step 2), filters off melt cinder, obtains
Extracting solution 2 mixes extracting solution 1 and extracting solution 2；
4) it is centrifuged: extracting solution mixed in step 3) being subjected to high speed refrigerated centrifuge, is mentioned with obtaining the higher safflower of purity
Take liquid；
5) it is concentrated: the extracting solution in step 4) being concentrated using Rotary Evaporators, keep extracting solution quality after concentration initial
10 times of safflower quality, to obtain the higher crocus sativus of concentration；
6) a certain amount of bacteriostatic agent is added in the extracting solution in step 5).
3. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that drying in step 1)
Time is 10-12h, and crossing grit number is 40-60 mesh.
4. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that safranine in step 2)
Colored and ethanol solution feed liquid mass ratio is 1: 30-50.
5. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that step 2) and 3) in
Extracting ethanol solution mass fraction used is all 40%-70%.
6. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that step 2) and 3) in
Ultrasonic temperature is all 40-60 DEG C.
7. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that step 2) and 3) in
Extraction time is all 40-60min.
8. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that extracted in step 4)
Liquid high speed freezing centrifuging temperature is 4 DEG C, centrifugation time 5-10min, revolving speed 9000rpm.
9. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that concentration in step 5)
Temperature is 40-60 DEG C.
10. a kind of extracting method of safflower active constituent according to claim 2, which is characterized in that in step 6)
Bacteriostatic agent is bright string coccus/radish root tunning filtrate, additional amount 2-3%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114146042A (en) * | 2021-09-18 | 2022-03-08 | 西行班达米尔(海南经济特区)生物科技有限公司 | Saffron crocus fermentation product and preparation method and application thereof |
| CN114515001A (en) * | 2021-08-31 | 2022-05-20 | 陕西佰瑞衡健康科技有限公司 | Preparation method of fermented saffron exosomes |
| CN114736736A (en) * | 2022-04-15 | 2022-07-12 | 甘肃烟草工业有限责任公司 | Saffron spice and preparation method and application thereof |
| CN115028668A (en) * | 2022-06-15 | 2022-09-09 | 西藏天虹科技股份有限责任公司 | Extraction process of crocin |
-
2019
- 2019-06-06 CN CN201910491529.0A patent/CN110251596A/en active Pending
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| 刘文娟等: "无防腐剂护肤化妆品体系的防腐效能及安全性评价", 《日用化学工业》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114515001A (en) * | 2021-08-31 | 2022-05-20 | 陕西佰瑞衡健康科技有限公司 | Preparation method of fermented saffron exosomes |
| CN114146042A (en) * | 2021-09-18 | 2022-03-08 | 西行班达米尔(海南经济特区)生物科技有限公司 | Saffron crocus fermentation product and preparation method and application thereof |
| CN114146042B (en) * | 2021-09-18 | 2023-11-10 | 西行班达米尔(海南经济特区)生物科技有限公司 | Saffron crocus fermentation product and preparation method and application thereof |
| CN114736736A (en) * | 2022-04-15 | 2022-07-12 | 甘肃烟草工业有限责任公司 | Saffron spice and preparation method and application thereof |
| CN114736736B (en) * | 2022-04-15 | 2023-08-04 | 甘肃烟草工业有限责任公司 | Saffron perfume and preparation method and application thereof |
| CN115028668A (en) * | 2022-06-15 | 2022-09-09 | 西藏天虹科技股份有限责任公司 | Extraction process of crocin |
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Application publication date: 20190920 |