CN103497838A - Method for extracting peony essential oil from peonies - Google Patents
Method for extracting peony essential oil from peonies Download PDFInfo
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- CN103497838A CN103497838A CN201310506233.4A CN201310506233A CN103497838A CN 103497838 A CN103497838 A CN 103497838A CN 201310506233 A CN201310506233 A CN 201310506233A CN 103497838 A CN103497838 A CN 103497838A
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Abstract
The invention relates to a method for extracting peony essential oil from peonies. The method comprises the following steps that step 1, the peonies are picked, forced air drying is conducted on the peonies, and the dried peonies are taken out and ground; step 2, a cellulase water solution and a pectinase water solution are mixed, acetum is added, and therefore a phosphoesterases complex solution is obtained; step 3, the ground peonies are placed in a triangular flask, the phosphoesterases complex solution is added, standing is conducted, ultrasonic treatment is conducted, and standing is conducted; step 4, the obtained solution in the step 3 is added to a petroleum ether ethanol solution, backflow is conducted, digestion is conducted at the indoor temperature, and the obtained solution is transferred into a rotary evaporator for vacuum concentration. According to the method, enzyme treatment is firstly conducted on the peonies so that local changes of loosening, expansion, crashing and the like are generated in the cell walls of the peonies and the mesenchyme structures of the cell walls; then, ultrasonic treatment is conducted on the peonies for a short time, and therefore the extraction rate of the essential oil from the cells of the peonies is greatly improved.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to a kind of method of extracting peony essential oil from Tree Peony flower.
Background technology
Peony essential oil is to extract plant essence flower from tree peony, leaf, root, fruit, is rich in amino acid and trace element that multiple human body needs.It can effectively be removed oxyradical and suppress skin pigment formation, smoothing wrinkle, minimizing skin splash and the pregnant occasion line of women.Improve blood microcirculation, remove the skin toxin, improve dry skin and pore refining, make skin smooth, flexible.Peony essential oil is natural plant hormone, and hormone secretion in balance women's body is alleviated menalgia, nourishes uterus.Anti-oxidant: distinctive tree peony polyphenol in peony essential oil is very strong antioxidant, can also extend other antioxidants time in vivo, delay skin aging.Suppress color spot, senile plaque, prevent and treat acne simultaneously, bright and clean skin quality.Therefore study a kind of method of extracting fast and efficiently peony essential oil from Tree Peony flower significant.
The extracting method of peony essential oil has four kinds of methods at present, and steam distillation, be damaged the effective constituent of peony essential oil, causes the yield of peony essential oil and quality to descend.The organic solvent extraction method needs the time long, the waste extensive work time.The operating process of adsorbing and extracting method is many, and the production cycle is long, production efficiency is low.The supercritical carbon dioxide extraction method has to technical process that strict demand, required condition are high, apparatus expensive.
Summary of the invention
The object of the present invention is to provide a kind of method of extracting peony essential oil from Tree Peony flower, can extract fast and efficiently peony essential oil from Tree Peony flower.
The technical solution used in the present invention is that a kind of method of extracting peony essential oil from Tree Peony flower comprises the following steps:
Step 1, ripe, the disease-free Tree Peony flower of harvesting, be placed in by Tree Peony flower the baking oven forced air drying that temperature is 25～30 ℃, takes out afterwards Tree Peony flower and be crushed to 40 orders, standby;
The polygalacturonase aqueous solution equal-volume that step 2, the cellulase aqueous solution that is 0.2mg/mL by mass concentration and mass concentration are 0.1mg/mL mixes, add the acetum that mass percent concentration is 2% in mixed solution, until the pH of mixed value reaches 5.0, by the mixed solution rear standing 10～15min that stirs, obtain prozyme liquid;
Step 3, the Tree Peony flower that step 1 is obtained are put into triangular flask, the prozyme liquid that adds step 2 to prepare in triangular flask, the ratio of prozyme liquid and Tree Peony flower add-on is 45:1, wherein prozyme liquid is in milliliter, Tree Peony flower is in gram, first will be placed into the triangular flask of Tree Peony flower standing 20min under the condition that temperature is 35 ℃ afterwards, then keep this temperature, and triangular flask is applied to the ultrasonication 5min that frequency is 22 kHz, finally triangular flask is transferred to standing 40min under the condition that temperature is 40 ℃, standby;
Step 4, in the triangular flask after standing, add refluxing extraction 1h in the sherwood oil ethanolic soln that mass concentration is 2～3kg/L, sherwood oil ethanolic soln add-on is 5 times of solution in triangular flask, lixiviate 1h under room temperature, concentrating under reduced pressure in the Rotary Evaporators that to proceed to afterwards temperature be 40 ~ 45 ℃, under the condition that to be concentrated into temperature be 40 ~ 45 ℃, relative density is 1.0g/mL.
Beneficial effect of the present invention has the following aspects:
One, under the condition that the present invention is first 35 ℃ in temperature, the Tree Peony flower enzyme is processed to 20min, can make the variations such as loose, expansion, collapse of Tree Peony flower cell walls and interstitial structure generation part thereof; Afterwards Tree Peony flower is applied the ultrasonication of short period of time, ultrasonic wave produces strong shockwave and microjet in the inside of liquid, this powerful impingement flow can effectively reduce, eliminate the block layer between solvent and water, thereby strengthened rate of mass transfer, in addition, impingement flow produces a kind of physics shearing force to cell tissue, make it distortion, break, and discharge inclusion, cell walls and interstitial structure generation part thereof loose, expand, this has accelerated the extraction process of peony essential oil in the cell greatly, has played the effect of getting twice the result with half the effort; After ultrasonication, Tree Peony flower is transferred under the condition that temperature is 40 ℃ and continues enzymolysis 40min, can make cell walls and interstitial thereof entirely collapse, greatly improved the extraction yield of peony essential oil in the Tree Peony flower cell.
Two, the ultrasonic frequency in the present invention is only 22kHz, can make the active centre of cellulase and polygalacturonase be moved, hydrophobicity is strengthened, while promoting enzyme digestion reaction and the better combination of substrate, promote Tree Peony flower cell walls and interstitial structure thereof to collapse faster, intracellular peony essential oil is entered in solvent faster.
Three, the present invention, by destroying cell walls and interstitial structure thereof, can make peony essential oil enter into fast solvent, has greatly shortened extraction time, has also improved the extraction yield of peony essential oil simultaneously.
Four, the present invention, by taking to take enzyme extraction as main, ultrasonic extraction are auxiliary method, while having avoided use ultrasonic extraction sample size large, arrives the ultrasonic energy decay of sample interior seriously, the problem that extraction effect is poor.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented take technical solution of the present invention under prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
A kind of method of extracting peony essential oil from Tree Peony flower comprises the following steps:
Step 1, ripe, the disease-free Tree Peony flower of harvesting, be placed in by Tree Peony flower the baking oven forced air drying that temperature is 25～30 ℃, takes out afterwards Tree Peony flower and be crushed to 40 orders, standby;
The polygalacturonase aqueous solution equal-volume that step 2, the cellulase aqueous solution that is 0.2mg/mL by mass concentration and mass concentration are 0.1mg/mL mixes, add the acetum that mass percent concentration is 2% in mixed solution, until the pH of mixed value reaches 5.0, by the mixed solution rear standing 10～15min that stirs, obtain prozyme liquid;
Step 3, the Tree Peony flower that step 1 is obtained are put into triangular flask, the prozyme liquid that adds step 2 to prepare in triangular flask, the ratio of prozyme liquid and Tree Peony flower add-on is 45:1, wherein prozyme liquid is in milliliter, Tree Peony flower is in gram, first will be placed into the triangular flask of Tree Peony flower standing 20min under the condition that temperature is 35 ℃ afterwards, then keep this temperature, and triangular flask is applied to the ultrasonication 5min that frequency is 22 kHz, finally triangular flask is transferred to standing 40min under the condition that temperature is 40 ℃, standby;
Step 4, in the triangular flask after standing, add refluxing extraction 1h in the sherwood oil ethanolic soln that mass concentration is 2～3kg/L, sherwood oil ethanolic soln add-on is 5 times of solution in triangular flask, lixiviate 1h under room temperature, concentrating under reduced pressure in the Rotary Evaporators that to proceed to afterwards temperature be 40 ~ 45 ℃, under the condition that to be concentrated into temperature be 40 ~ 45 ℃, relative density is 1.0g/mL.
Embodiment 1
A kind of method of extracting peony essential oil from Tree Peony flower comprises the following steps:
Step 1, ripe, the disease-free Tree Peony flower of harvesting, be placed in by Tree Peony flower the baking oven forced air drying that temperature is 30 ℃, takes out afterwards Tree Peony flower and be crushed to 40 orders, standby;
The polygalacturonase aqueous solution equal-volume that step 2, the cellulase aqueous solution that is 0.2mg/mL by mass concentration and mass concentration are 0.1mg/mL mixes, add the acetum that mass percent concentration is 2% in mixed solution, until the pH of mixed value reaches 5.0, by the mixed solution rear standing 12min that stirs, obtain prozyme liquid;
Step 3, the Tree Peony flower that step 1 is obtained are put into triangular flask, the prozyme liquid that adds step 2 to prepare in triangular flask, the ratio of prozyme liquid and Tree Peony flower add-on is 45:1, wherein prozyme liquid is in milliliter, Tree Peony flower is in gram, first will be placed into the triangular flask of Tree Peony flower standing 20min under the condition that temperature is 35 ℃ afterwards, then keep this temperature, and triangular flask is applied to the ultrasonication 5min that frequency is 22 kHz, finally triangular flask is transferred to standing 40min under the condition that temperature is 40 ℃, standby;
Step 4, in the triangular flask after standing, add refluxing extraction 1h in the sherwood oil ethanolic soln that mass concentration is 2.5kg/L, sherwood oil ethanolic soln add-on is 5 times of solution in triangular flask, lixiviate 1h under room temperature, concentrating under reduced pressure in the Rotary Evaporators that to proceed to afterwards temperature be 42 ℃, under the condition that to be concentrated into temperature be 42 ℃, relative density is 1.0g/mL.
Experimental result shows: the present invention compares with organic solvent extraction method commonly used, and extraction time has shortened one hour, and extraction yield has raise 13%, has obtained outstanding significant progressive.
Claims (1)
1. a method of extracting peony essential oil from Tree Peony flower, is characterized in that, comprises the following steps:
Step 1, ripe, the disease-free Tree Peony flower of harvesting, be placed in by Tree Peony flower the baking oven forced air drying that temperature is 25～30 ℃, takes out afterwards Tree Peony flower and be crushed to 40 orders, standby;
The polygalacturonase aqueous solution equal-volume that step 2, the cellulase aqueous solution that is 0.2mg/mL by mass concentration and mass concentration are 0.1mg/mL mixes, add the acetum that mass percent concentration is 2% in mixed solution, until the pH of mixed value reaches 5.0, by the mixed solution rear standing 10～15min that stirs, obtain prozyme liquid;
Step 3, the Tree Peony flower that step 1 is obtained are put into triangular flask, the prozyme liquid that adds step 2 to prepare in triangular flask, the ratio of prozyme liquid and Tree Peony flower add-on is 45:1, wherein prozyme liquid is in milliliter, Tree Peony flower is in gram, first will be placed into the triangular flask of Tree Peony flower standing 20min under the condition that temperature is 35 ℃ afterwards, then keep this temperature, and triangular flask is applied to the ultrasonication 5min that frequency is 22 kHz, finally triangular flask is transferred to standing 40min under the condition that temperature is 40 ℃, standby;
Step 4, in the triangular flask after standing, add refluxing extraction 1h in the sherwood oil ethanolic soln that mass concentration is 2～3kg/L, sherwood oil ethanolic soln add-on is 5 times of solution in triangular flask, lixiviate 1h under room temperature, concentrating under reduced pressure in the Rotary Evaporators that to proceed to afterwards temperature be 40 ~ 45 ℃, under the condition that to be concentrated into temperature be 40 ~ 45 ℃, relative density is 1.0g/mL.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784523A (en) * | 2014-01-28 | 2014-05-14 | 齐鲁工业大学 | Peony polyphenol extraction process |
| CN104277915A (en) * | 2014-09-25 | 2015-01-14 | 齐鲁工业大学 | Method for extracting peony essential oil from peony |
| CN107308100A (en) * | 2017-07-21 | 2017-11-03 | 江苏岩藤农业发展有限公司 | A kind of additive, its preparation method and the peony essential oil comprising it that prevent peony essential oil from volatilizing |
| CN109588722A (en) * | 2019-02-13 | 2019-04-09 | 山东贝世康生物科技有限公司 | A kind of compound tree peony seed oil soft capsule and preparation method thereof with blood pressure reduction effect |
| CN109730948A (en) * | 2019-01-24 | 2019-05-10 | 山东贝世康生物科技有限公司 | The method and application of fresh peony flower cellular water are extracted from fresh peony flower |
| CN116240067A (en) * | 2023-03-13 | 2023-06-09 | 菏泽牡丹产业技术研究院 | Peony seed crude oil, refined peony seed oil, and preparation methods and applications thereof |
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| CN107308100A (en) * | 2017-07-21 | 2017-11-03 | 江苏岩藤农业发展有限公司 | A kind of additive, its preparation method and the peony essential oil comprising it that prevent peony essential oil from volatilizing |
| CN109730948A (en) * | 2019-01-24 | 2019-05-10 | 山东贝世康生物科技有限公司 | The method and application of fresh peony flower cellular water are extracted from fresh peony flower |
| CN109588722A (en) * | 2019-02-13 | 2019-04-09 | 山东贝世康生物科技有限公司 | A kind of compound tree peony seed oil soft capsule and preparation method thereof with blood pressure reduction effect |
| CN109588722B (en) * | 2019-02-13 | 2022-10-14 | 山东贝世康生物科技有限公司 | Compound peony seed oil soft capsule with blood pressure lowering effect and preparation method thereof |
| CN116240067A (en) * | 2023-03-13 | 2023-06-09 | 菏泽牡丹产业技术研究院 | Peony seed crude oil, refined peony seed oil, and preparation methods and applications thereof |
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