CN104130862A - Extraction method of indocalamus leaf volatile oil - Google Patents
Extraction method of indocalamus leaf volatile oil Download PDFInfo
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- CN104130862A CN104130862A CN201410266388.XA CN201410266388A CN104130862A CN 104130862 A CN104130862 A CN 104130862A CN 201410266388 A CN201410266388 A CN 201410266388A CN 104130862 A CN104130862 A CN 104130862A
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- volatile oil
- indicalamus leaf
- extraction
- indicalamus
- leaf volatile
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Abstract
The invention relates to an extraction method of an indocalamus leaf volatile oil, and belongs to the field of plant volatile oil extraction. The extraction method comprises the processing steps: fermenting an indocalamus leaf powder by cellulase; after fermentation, extracting the raw material by an ultrasonic-microwave method; and leaching the extraction liquid with an organic solvent, drying with anhydrous sodium sulfate, carrying out reduced pressure concentration and other steps, and thus obtaining the indocalamus leaf volatile oil. The extraction method has the advantages of simple process, small energy consumption, mild reaction conditions, and high yield of the product, and overcomes the shortcomings that a traditional steam distillation method wastes time and energy and a product is easy to lose.
Description
Technical field
The present invention relates to a kind of extracting method of indicalamus leaf volatile oil, be specifically related to the method for a kind of enzymic fermentation in conjunction with Ultrasound-microwave assisted extraction indicalamus leaf volatile oil.
Background technology
Indicalamus leaf is commonly called as rice-pudding leaf, and it is the blade of the broadleaf indocalamus during Gramineae Bambusoideae indocalamus belongs to.Cure mainly that throat closes that pain, lung carbuncle, nose is bleeding, menstruation are more than, discharging fresh blood stool, urinary obstruction, and for women's lactagogue etc.The natural flavor that the distinctive rice-pudding fragrance of indicalamus leaf is a kind of pleasant, meets the requirement that modern goes back to nature.Therefore the volatile oil, extracting from indicalamus leaf can have broad application prospects at the development field of the products such as natural essence, foodstuff additive, makeup, reodorant and air freshener.
The common process that extracts at present indicalamus leaf volatile component is steam distillation, supercritical extraction etc.Extraction by steam distillation temperature is high, and the time is long, easily make damp and hot unsettled volatile component change, and high boiling composition is difficult to detect; And supercritical fluid extraction is a kind of new extracting method, extract volatile oil ability strong, there is anti-oxidation, pyrolysis and improve the advantages such as volatile oil quality, but this technique needs high-tension apparatus, technical requirements is high, and cost of equipment investment is large, applies also not general.
By literature search, have no the relevant report of enzymic fermentation in conjunction with Ultrasound-microwave assisted extraction indicalamus leaf volatile oil.
Summary of the invention
The object of the present invention is to provide the method for the extraction indicalamus leaf volatile oil that a kind of technique is simple, extraction yield is high and product quality is good.
The present invention includes following steps:
A. by indicalamus leaf drying and crushing, cross 20 mesh sieves;
B. get in the citric acid/sodium citrate buffered soln of pH4.0 ~ 5.0 that the cellulase that is equivalent to indicalamus leaf powder weight 1 ~ 3% is dissolved in 0.5 ~ 1 times, by gained spray solution, in indicalamus leaf powder, diaphragm seal, in 45 ~ 55 DEG C of fermentations 3 ~ 5 days;
C. the raw material after fermentation is added to 60 ~ 70% the ethanol of 3 ~ 5 times, under the condition that ultrasonic power 600W often opens, microwave power is 600 ~ 800W, extract 1.5 ~ 2.5h;
D. filter, filtrate is used organic solvent extraction 2 ~ 3 times, combining extraction liquid, then through anhydrous sodium sulfate drying dehydration, concentrating under reduced pressure, obtain indicalamus leaf volatile oil.
The activity of the cellulase described in step b is 30 ~ 60u/mg.
Organic solvent described in steps d is the one in sherwood oil, ether.
Beneficial effect of the present invention is: (1) the present invention adopts enzymic fermentation technology, destroys the cell walls of raw material, increases the stripping of effective constituent; (2) adopt modern Ultrasound-microwave assisted extraction indicalamus leaf volatile oil, its yield is apparently higher than traditional steam distillation, and superior product quality; (3) save time, energy-conservation, and environmental protection.
Further illustrate the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to following embodiment.
embodiment:
Embodiment 1:
By indicalamus leaf drying and crushing, cross 20 mesh sieves, get 500g powder; Get in the citric acid/sodium citrate buffered soln of pH4.0 that the cellulase (active for 30u/mg) that is equivalent to indicalamus leaf powder weight 1% is dissolved in 0.5 times, by gained spray solution, in indicalamus leaf powder, diaphragm seal, in 45 DEG C of fermentations 3 days; Raw material after fermentation is added to 60% the ethanol of 3 times, under the condition that ultrasonic power 600W often opens, microwave power is 600W, extract 1.5h; Filter, filtrate is used petroleum ether extraction 2 times, combining extraction liquid, then through anhydrous sodium sulfate drying dehydration, concentrating under reduced pressure, obtain indicalamus leaf volatile oil 4.5mL.
Embodiment 2:
By indicalamus leaf drying and crushing, cross 20 mesh sieves, get 500g powder; Get in the citric acid/sodium citrate buffered soln of pH4.5 that the cellulase (active for 60u/mg) that is equivalent to indicalamus leaf powder weight 3% is dissolved in 1 times, by gained spray solution, in indicalamus leaf powder, diaphragm seal, in 55 DEG C of fermentations 5 days; Raw material after fermentation is added to 65% the ethanol of 4 times, under the condition that ultrasonic power 600W often opens, microwave power is 800W, extract 2h; Filter, filtrate is used extracted with diethyl ether 2 times, combining extraction liquid, then through anhydrous sodium sulfate drying dehydration, concentrating under reduced pressure, obtain indicalamus leaf volatile oil 5.8mL.
Embodiment 3:
By indicalamus leaf drying and crushing, cross 20 mesh sieves, get 500g powder; Get in the citric acid/sodium citrate buffered soln of pH5.0 that the cellulase (active for 50u/mg) that is equivalent to indicalamus leaf powder weight 2% is dissolved in 0.5 times, by gained spray solution, in indicalamus leaf powder, diaphragm seal, in 50 DEG C of fermentations 4 days; Raw material after fermentation is added to 70% the ethanol of 5 times, under the condition that ultrasonic power 600W often opens, microwave power is 700W, extract 2.5h; Filter, filtrate is used petroleum ether extraction 3 times, combining extraction liquid, then through anhydrous sodium sulfate drying dehydration, concentrating under reduced pressure, obtain indicalamus leaf volatile oil 5.1mL.
Claims (3)
1. an extracting method for indicalamus leaf volatile oil, is characterized in that, comprises the following steps:
A. by indicalamus leaf drying and crushing, cross 20 mesh sieves;
B. get in the citric acid/sodium citrate buffered soln of pH4.0 ~ 5.0 that the cellulase that is equivalent to indicalamus leaf powder weight 1 ~ 3% is dissolved in 0.5 ~ 1 times, by gained spray solution, in indicalamus leaf powder, diaphragm seal, in 45 ~ 55 DEG C of fermentations 3 ~ 5 days;
C. the raw material after fermentation is added to 60 ~ 70% the ethanol of 3 ~ 5 times, under the condition that ultrasonic power 600W often opens, microwave power is 600 ~ 800W, extract 1.5 ~ 2.5h;
D. filter, filtrate is used organic solvent extraction 2 ~ 3 times, combining extraction liquid, then through anhydrous sodium sulfate drying dehydration, concentrating under reduced pressure, obtain indicalamus leaf volatile oil.
2. the extracting method of a kind of indicalamus leaf volatile oil according to claim 1, is characterized in that, the activity of the cellulase described in step b is 30 ~ 60u/mg.
3. the extracting method of a kind of indicalamus leaf volatile oil according to claim 1, is characterized in that, the organic solvent described in steps d is the one in sherwood oil, ether.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410266388.XA CN104130862A (en) | 2014-06-16 | 2014-06-16 | Extraction method of indocalamus leaf volatile oil |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410266388.XA CN104130862A (en) | 2014-06-16 | 2014-06-16 | Extraction method of indocalamus leaf volatile oil |
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| CN104130862A true CN104130862A (en) | 2014-11-05 |
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| CN201410266388.XA Pending CN104130862A (en) | 2014-06-16 | 2014-06-16 | Extraction method of indocalamus leaf volatile oil |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106377481A (en) * | 2016-11-17 | 2017-02-08 | 东莞波顿香料有限公司 | Extraction method of cyperus rotundus extract and toothpaste and mouth wash containing cyperus rotundus extract |
| CN107952095A (en) * | 2017-12-26 | 2018-04-24 | 安徽仁维环保工程科技有限公司 | A kind of preparation method of bamboo leaf powder for deodorization |
| CN112385774A (en) * | 2020-11-09 | 2021-02-23 | 颍上县皖润米业有限公司 | Processing method of health-care rice with bamboo fragrance |
| CN113967188A (en) * | 2021-12-10 | 2022-01-25 | 杭州雅妍化妆品有限公司 | Preparation method and application of bamboo leaf fermentation filtrate |
-
2014
- 2014-06-16 CN CN201410266388.XA patent/CN104130862A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106377481A (en) * | 2016-11-17 | 2017-02-08 | 东莞波顿香料有限公司 | Extraction method of cyperus rotundus extract and toothpaste and mouth wash containing cyperus rotundus extract |
| CN107952095A (en) * | 2017-12-26 | 2018-04-24 | 安徽仁维环保工程科技有限公司 | A kind of preparation method of bamboo leaf powder for deodorization |
| CN112385774A (en) * | 2020-11-09 | 2021-02-23 | 颍上县皖润米业有限公司 | Processing method of health-care rice with bamboo fragrance |
| CN113967188A (en) * | 2021-12-10 | 2022-01-25 | 杭州雅妍化妆品有限公司 | Preparation method and application of bamboo leaf fermentation filtrate |
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Application publication date: 20141105 |