CN105412245A - Extraction process of cassia twig and application of extraction process - Google Patents
Extraction process of cassia twig and application of extraction process Download PDFInfo
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- CN105412245A CN105412245A CN201510889395.XA CN201510889395A CN105412245A CN 105412245 A CN105412245 A CN 105412245A CN 201510889395 A CN201510889395 A CN 201510889395A CN 105412245 A CN105412245 A CN 105412245A
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- CN
- China
- Prior art keywords
- extraction process
- ramulus cinnamomi
- carry out
- cassia twig
- concentrated
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention provides an extraction process of cassia twig and application of extraction process. The extraction process comprises the following operating steps: 1) digesting: cleaning the cassia twig with impurities removed with clean water, smashing the cassia twig and digesting the smashed cassia twig in a digester, so that a mixture is obtained; 2) water-extracting: filtering an obtained mixed liquid by virtue of filter cloth after heating and digesting the mixture for a duration, so that a water extract is obtained; 3) finely extracting: separating and centrifuging the obtained water extract once again so as to obtain a supernatant; 4) concentrating: concentrating the supernatant by virtue of a rotary evaporator in a vacuum environment, so that a concentrated solution is obtained; 5) rinsing: after concentrating, centrifuging the concentrated solution, removing supernatant, and rinsing by virtue of an organic solvent; and 6) drying: drying a rinsed product obtained in the step 5) at low temperature, so that the extraction process is completed. The entire processing technology achieves the full extraction of traditional Chinese medicinal ingredients in the cassia twig, so that the medicinal value of an extract is greatly improved, and the extract plays an important role in important carcinogenic drugs; and the extraction process is more suitable for production than a conventional process.
Description
Technical field
The present invention relates to Chinese medicinal material field, be specifically related to a kind of extraction process and application thereof of Ramulus Cinnamomi.
Background technology
On Chinese Clinical, Chinese medicine has been widely used and has obtained very ten-strike.The disease that different Chinese herbal medicine combined therapies is different.But treatment by Chinese herbs mechanism is still not very clear.In many Chinese medicine formulas, Ramulus Cinnamomi is used, such as, treat arthralgia, numb limbs and tense tendons, alleviates adhesion, treatment amenorrhea and menorrhagia etc.Recent studies have shown that Ramulus Cinnamomi has unique curative effect in Tumor suppression.In order to the biologic activity at molecular biology horizontal detection Ramulus Cinnamomi, and then illustrate Ramulus Cinnamomi extract antineoplastic mechanism, we study Ramulus Cinnamomi extract, i.e. TIMP.By the mode of organic solvent extraction, the water extract of Ramulus Cinnamomi is produced.
Matrix metalloproteinase is the dependent high conservative proteolytic enzyme of zinc, can degradation of cell epimatrix and basement membrane.The exception of stromatin enzyme unconventionality expression and function, will cause the growth of cancer cell, invasion and attack, and transfer and new vessels formation, these phenomenons attract numerous research worker that sight is put into the target spot that Proteolytic enzyme enzyme family studies as treatment of cancer.Nearly ten years, a series of efficient matrix inhibitors of metalloproteinase has been widely deployed and has been applied in clinical experiment, but significantly side effect and non-specific seriously limit development.Therefore, developing efficient and that side effect is little matrix metallo-proteinase inhibitor will be very promising research work.
Come in, many Chinese herbal medicine have been proved to be the activity or expression that can suppress stromatin enzyme, and then the propagation of anticancer, transfer and invasion and attack.Some researchs find that Fructus Gardeniae water solubility extract is also inhibited to the activity of stromatin enzyme.In addition, some compounds separated from Chinese herbal medicine are proved the activity that effectively can suppress matrix metalloproteinase.
Ramulus Cinnamomi is as a kind of traditional important Chinese medicine material, and also usually add in some Chinese medicine formulas, but be that how to produce antitumor mechanism to human body be not also very clear and definite for which kind of material in Ramulus Cinnamomi, usually need a large amount of blindnesses to add edible, and really play the still very limited of beneficial effect.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of extraction process and application thereof of Ramulus Cinnamomi, the effective important component in Ramulus Cinnamomi is fully refined, greatly can improve the extraction of Ramulus Cinnamomi in production and processing link, strengthen its medical value.
For realizing above object, the present invention is achieved by the following technical programs:
1, an extraction process for Ramulus Cinnamomi, operating procedure is as follows:
1) steaming and decocting: by removing the Ramulus Cinnamomi of foreign material, by clean water, putting into digester after smashing to pieces and carrying out steaming and decocting, obtaining mixture;
2) water extraction: after hot digestion a period of time, by mixed liquor filter-cloth filtering, obtains Aqueous extracts;
3) essence is extracted: the Aqueous extracts obtained is carried out separation centrifugal treating again, obtains supernatant;
4) concentrated: to use rotary evaporator to concentrate under vacuo, obtain concentrated solution;
5) rinse: by after concentrated concentrated solution carrying out centrifugally operated, remove supernatant, carry out rinse operation with organic solvent;
6) dry: by step 5) after rinse product carry out drying at low temperatures.
Preferably, described step 1) steaming and decocting operation to carry out 3 times.
Preferably, described step 1) control when heating to carry out magnetic agitation simultaneously.
Preferably, described step 2) add a small amount of NaOH solution in water extraction operation, adjust ph is 8.
Preferably, described step 4) carry out dialysis operation after concentrated maneuvering sequence, add hydrochloric acid in extracting solution, adjust ph is 7.2, dialyses with bag filter.
Preferably, described step 5) in the organic solvent that adopts be petroleum ether, chloroform, one or more in ethyl acetate and bytyry ethanol.
The invention provides a kind of extraction process and application thereof of Ramulus Cinnamomi, extracted by steaming and decocting, water extraction, essence, concentrated, rinse, drying, effectively extracted by the active drug composition in Ramulus Cinnamomi, more traditional extraction process efficiency is higher, and by concentrated, rinse, dialysis operation, the extract of Ramulus Cinnamomi effectively can be preserved, do not damage the property of medicine, make its extract have higher antiinflammatory, antiallergic, antiviral, antibacterial., the Ramulus Cinnamomi composition extracted can be used in cancer therapy drug, can produce important medical value, and extraction step of the present invention is also more applicable for industrialized production and processing, can further industrialized production and further genralrlization.
Detailed description of the invention
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with embodiments of the invention, the technical scheme in the embodiment of the present invention is clearly and completely described.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1:
The present embodiment provides a kind of extraction process of Ramulus Cinnamomi, and operating procedure is as follows:
1) steaming and decocting: by removing the Ramulus Cinnamomi of foreign material, by clean water, putting into digester after smashing to pieces and carrying out steaming and decocting, obtaining mixture;
2) water extraction: after hot digestion a period of time, by mixed liquor filter-cloth filtering, obtains Aqueous extracts;
3) essence is extracted: the Aqueous extracts obtained is carried out separation centrifugal treating again, obtains supernatant;
4) concentrated: to use rotary evaporator to concentrate under vacuo, obtain concentrated solution;
5) rinse: by after concentrated concentrated solution carrying out centrifugally operated, remove supernatant, carry out rinse operation with organic solvent;
6) dry: by step 5) after rinse product carry out drying at low temperatures.
Step 1) steaming and decocting operation to carry out 3 times.
Step 1) control when heating to carry out magnetic agitation simultaneously.
Step 2) water extraction operation in add a small amount of NaOH solution, adjust ph is 8.
Step 4) carry out dialysis operation after concentrated maneuvering sequence, add hydrochloric acid in extracting solution, adjust ph is 7.2, dialyses with bag filter.
Step 5) in the organic solvent that adopts be the mixing of petroleum ether, chloroform.
Embodiment 2:
The present embodiment provides a kind of extraction process of Ramulus Cinnamomi, and operating procedure is as follows:
1) steaming and decocting: by removing the Ramulus Cinnamomi of foreign material, by clean water, putting into digester after smashing to pieces and carrying out steaming and decocting, obtaining mixture;
2) water extraction: after hot digestion a period of time, by mixed liquor filter-cloth filtering, obtains Aqueous extracts;
3) essence is extracted: the Aqueous extracts obtained is carried out separation centrifugal treating again, obtains supernatant;
4) concentrated: to use rotary evaporator to concentrate under vacuo, obtain concentrated solution;
5) rinse: by after concentrated concentrated solution carrying out centrifugally operated, remove supernatant, carry out rinse operation with organic solvent;
6) dry: by step 5) after rinse product carry out drying at low temperatures.
Step 1) control when heating to carry out magnetic agitation simultaneously.
Step 2) water extraction operation in add a small amount of NaOH solution, adjust ph is 8.
Step 4) carry out dialysis operation after concentrated maneuvering sequence, add hydrochloric acid in extracting solution, adjust ph is 7.2, dialyses with bag filter.
Step 5) in adopt organic solvent be bytyry ethanol.
Embodiment 3:
The present embodiment provides a kind of extraction process of Ramulus Cinnamomi, and operating procedure is as follows:
1) steaming and decocting: by removing the Ramulus Cinnamomi of foreign material, by clean water, putting into digester after smashing to pieces and carrying out steaming and decocting, obtaining mixture;
2) water extraction: after hot digestion a period of time, by mixed liquor filter-cloth filtering, obtains Aqueous extracts;
3) essence is extracted: the Aqueous extracts obtained is carried out separation centrifugal treating again, obtains supernatant;
4) concentrated: to use rotary evaporator to concentrate under vacuo, obtain concentrated solution;
5) rinse: by after concentrated concentrated solution carrying out centrifugally operated, remove supernatant, carry out rinse operation with organic solvent;
6) dry: by step 5) after rinse product carry out drying at low temperatures.
Step 1) steaming and decocting operation to carry out 3 times.
Step 2) water extraction operation in add a small amount of NaOH solution, adjust ph is 8.
Step 4) carry out dialysis operation after concentrated maneuvering sequence, add hydrochloric acid in extracting solution, adjust ph is 7.2, dialyses with bag filter.
Step 5) in adopt organic solvent be ethyl acetate.
Above embodiment only in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (6)
1. an extraction process for Ramulus Cinnamomi, is characterized in that, operating procedure is as follows:
1) steaming and decocting: by removing the Ramulus Cinnamomi of foreign material, by clean water, putting into digester after smashing to pieces and carrying out steaming and decocting, obtaining mixture;
2) water extraction: after hot digestion a period of time, by mixed liquor filter-cloth filtering, obtains Aqueous extracts;
3) essence is extracted: the Aqueous extracts obtained is carried out separation centrifugal treating again, obtains supernatant;
4) concentrated: to use rotary evaporator to concentrate under vacuo, obtain concentrated solution;
5) rinse: by after concentrated concentrated solution carrying out centrifugally operated, remove supernatant, carry out rinse operation with organic solvent;
6) dry: by step 5) after rinse product carry out drying at low temperatures.
2. the extraction process of Ramulus Cinnamomi as claimed in claim 1, is characterized in that, described step 1) steaming and decocting operation to carry out 3 times.
3. the extraction process of Ramulus Cinnamomi as claimed in claim 1, is characterized in that, described step 1) control when heating to carry out magnetic agitation simultaneously.
4. the extraction process of Ramulus Cinnamomi as claimed in claim 1, is characterized in that, described step 2) add a small amount of NaOH solution in water extraction operation, adjust ph is 8.
5. the extraction process of Ramulus Cinnamomi as claimed in claim 1, is characterized in that, described step 4) concentrated handle after, then carry out dialysis operation, add hydrochloric acid in extracting solution, adjust ph is 7.2, dialyses with bag filter.
6. the extraction process of Ramulus Cinnamomi as claimed in claim 1, is characterized in that, described step 5) in the organic solvent that adopts be petroleum ether, chloroform, one or more in ethyl acetate and bytyry ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510889395.XA CN105412245A (en) | 2015-12-07 | 2015-12-07 | Extraction process of cassia twig and application of extraction process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510889395.XA CN105412245A (en) | 2015-12-07 | 2015-12-07 | Extraction process of cassia twig and application of extraction process |
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| CN105412245A true CN105412245A (en) | 2016-03-23 |
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| CN201510889395.XA Pending CN105412245A (en) | 2015-12-07 | 2015-12-07 | Extraction process of cassia twig and application of extraction process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106491696A (en) * | 2016-10-31 | 2017-03-15 | 秦冬妹 | The method for extracting toxin cyanide from Maninot esculenta crantz. |
| CN110585270A (en) * | 2019-10-16 | 2019-12-20 | 中山市道和生物科技有限公司 | Method for extracting active ingredients of cassia twig |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104292350A (en) * | 2014-09-17 | 2015-01-21 | 上海交通大学 | Extraction process and application of chrysanthemum polysaccharide |
-
2015
- 2015-12-07 CN CN201510889395.XA patent/CN105412245A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104292350A (en) * | 2014-09-17 | 2015-01-21 | 上海交通大学 | Extraction process and application of chrysanthemum polysaccharide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106491696A (en) * | 2016-10-31 | 2017-03-15 | 秦冬妹 | The method for extracting toxin cyanide from Maninot esculenta crantz. |
| CN110585270A (en) * | 2019-10-16 | 2019-12-20 | 中山市道和生物科技有限公司 | Method for extracting active ingredients of cassia twig |
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Application publication date: 20160323 |