CN104892695A - Preparation method for extracting tannin with camellia oleifera shells - Google Patents
Preparation method for extracting tannin with camellia oleifera shells Download PDFInfo
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- CN104892695A CN104892695A CN201410085241.0A CN201410085241A CN104892695A CN 104892695 A CN104892695 A CN 104892695A CN 201410085241 A CN201410085241 A CN 201410085241A CN 104892695 A CN104892695 A CN 104892695A
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Abstract
The present invention discloses a preparation method for extracting tannin with camellia oleifera shells, and relates to the technical field of biological extraction. According to the certain feed liquid ratio, the camellia oleifera shells, which are dried and crushed into powder, are added in a defined amount of ethanol solution; extraction, ultrasound auxiliary extraction, centrifugation, precipitation, spray drying and secondary extraction purification are conducted at a certain temperature to obtain the tannin. Compared with a conventional extraction method, the preparation method has the advantages of being short in extraction time and high in tannin extraction rate, and comprehensively utilizing camellia oleifera shell resource; through the technology, the extraction rate of the tannin can reach above 6.75%, protein purity degree is above 95%, and 67.5 kilograms of protein can be extracted in per ton of camellia oleifera seed cakes.
Description
Technical field
The present invention relates to technical field of biological extraction, particularly a kind of oil-tea camellia husks extracts the method for tannin.
Background technology
Oil tea is the important oilseed plant of one of south China knob, and China is that oil tea distribution is the widest in the world, and the country that kind is maximum, produce tea seed about 54.98 ten thousand tons per year, the annual output of oil tea industry byproduct---oil-tea camellia husks is about 600,000 tons.Tannin containing 6-7% in oil-tea camellia husks, tannin Bearberry Extract, for oral administration can be used for, treats gastrointestinal hemorrhage, the disease such as ulcer and watery diarrhea; Outer for wound, burn, can to make after wound protein coagulating in exudate, form scab film, can reduce secretion and protect from infection, tannin can make the capillary blood vessel of the surface of a wound shrink, and has local hemostasis effect.Tannin can solidify the protoplasma in microbe, therefore has bacteriostatic action, some tannin tool antivirus action, as rhizome of cyrtomium can suppress multiple influenza virus.Tannin can be used as toxinicide when alkaloid and some heavy metal poisoning.The reductibility that tannin tool is stronger, can remove the superoxide radical in organism, delay senility.In addition, tannin also has anti-allergic, anti-inflammatory, expelling parasite, the effect such as hypotensive, has very large Development volue.
Therefore utilize oil-tea camellia husks to be separated tannin for raw material, and it can be used as medical material to develop and utilize and be very important.
Summary of the invention
The technical problem to be solved in the present invention is: provide a kind of oil-tea camellia husks to extract the preparation method of tannin, and this preparation method extracts tannin, and to have extraction yield high, and product purity is high.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Oil-tea camellia husks extracts a preparation method for tannin, the steps include:
A, degreasing tea-seed pancake is placed in dry 1-2 hour at 40 DEG C of-60 DEG C of temperature, pulverizes, cross 60-100 mesh sieve, obtain degreasing tea-seed pancake powder;
B, by degreasing tea-seed pancake powder in 1: 10-30 ratio join and soak 40-80 minute in 40%-60% ethanolic soln after, with ultrasonic extraction 40-120 minute;
Add 30%-38% hydrochloric acid adjust pH to iso-electric point 3-6, centrifugal 10-30 minute in C, feed liquid after extraction, precipitation, abandoning supernatant, obtains throw out;
D, by throw out spraying dry, obtain tannin;
E, repeat extract 2-3 time, highly purified tannin can be obtained.
Described degreasing tea-seed pancake drying temperature is 50 DEG C, and time of drying is 1.5 hours.
Described degreasing tea-seed pancake and the solid-liquid ratio of ethanolic soln are 1: 20, and soak time is 60 minutes.
Described use ultrasonic extraction 80 minutes.
The described concentration of hydrochloric acid that adds is 35%, and adjust pH to iso-electric point is 4.5.
Described centrifugal rotational speed is 3000r/min, and centrifugation time is 20min.
The invention has the beneficial effects as follows:
In oil-tea camellia husks, content of tannin is about 6% ~ 7%, and tannin extraction yield of the present invention can reach more than 6.75%, and after second extraction, tannin purity is more than 95%, and tea-seed pancake per ton can extract 95% tannin 67.5 kilograms.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment one:
A, degreasing tea-seed pancake to be placed at 50 DEG C of temperature dry 1.5 hours, to pulverize, cross 80 mesh sieves, obtain degreasing tea-seed pancake powder;
B, by degreasing tea-seed pancake powder in 1: 20 ratio join in 50% ethanolic soln and soak after 60 minutes, by ultrasonic extraction 80 minutes;
Add 35% hydrochloric acid adjust pH to iso-electric point 4.5, centrifugal 20 minutes in C, feed liquid after extraction, precipitation, abandoning supernatant, obtains throw out;
D, by throw out spraying dry, obtain tannin;
E, repeat extract 2-3 time, highly purified tannin can be obtained.
Embodiment two:
A, degreasing tea-seed pancake to be placed at 40 DEG C of temperature dry 1 hour, to pulverize, cross 60 mesh sieves, obtain degreasing tea-seed pancake powder;
B, by degreasing tea-seed pancake powder in 1: 10 ratio join in 40% ethanolic soln and soak after 40 minutes, by ultrasonic extraction 40 minutes;
Add 30% hydrochloric acid adjust pH to iso-electric point 3, centrifugal 10 minutes in C, feed liquid after extraction, precipitation, abandoning supernatant, obtains throw out;
D, by throw out spraying dry, obtain tannin;
E, repeat extract 2-3 time, highly purified tannin can be obtained.
Embodiment three:
A, degreasing tea-seed pancake to be placed at 60 DEG C of temperature dry 2 hours, to pulverize, cross 100 mesh sieves, obtain degreasing tea-seed pancake powder;
B, by degreasing tea-seed pancake powder in 1: 30 ratio join in 60% ethanolic soln and soak after 80 minutes, by ultrasonic extraction 120 minutes;
Add 38% hydrochloric acid adjust pH to iso-electric point 6, centrifugal 30 minutes in C, feed liquid after extraction, precipitation, abandoning supernatant, obtains throw out;
D, by throw out spraying dry, obtain tannin;
E, repeat extract 2-3 time, highly purified tannin can be obtained.
Claims (6)
1. extract a preparation method for tannin with oil-tea camellia husks, the steps include:
A, degreasing tea-seed pancake is placed in dry 1-2 hour at 40 DEG C of-60 DEG C of temperature, pulverizes, cross 60-100 mesh sieve, obtain degreasing tea-seed pancake powder;
B, by degreasing tea-seed pancake powder in 1: 10-30 ratio join and soak 40-80 minute in 40%-60% ethanolic soln after, with ultrasonic extraction 40-120 minute;
Add 30%-38% hydrochloric acid adjust pH to iso-electric point 3-6, centrifugal 10-30 minute in C, feed liquid after extraction, precipitation, abandoning supernatant, obtains throw out;
D, by throw out spraying dry, obtain tannin;
E, by above-mentioned steps repeat extract 2-3 time, highly purified tannin can be obtained.
2. a kind of oil-tea camellia husks according to claim 1 extracts the preparation method of tannin, it is characterized in that: described degreasing tea-seed pancake drying temperature is 50 DEG C, and time of drying is 1.5 hours.
3. a kind of oil-tea camellia husks according to claim 1 extracts the preparation method of tannin, it is characterized in that: described degreasing tea-seed pancake and the solid-liquid ratio of ethanolic soln are 1: 20, and soak time is 60 minutes.
4. a kind of oil-tea camellia husks according to claim 1 extracts the preparation method of tannin, it is characterized in that: described use ultrasonic extraction 80 minutes.
5. a kind of oil-tea camellia husks according to claim 1 extracts the preparation method of tannin, it is characterized in that: the described concentration of hydrochloric acid that adds is 35%, and adjust pH to iso-electric point is 4.5.
6. a kind of oil-tea camellia husks according to claim 1 extracts the preparation method of tannin, and it is characterized in that: described centrifugal rotational speed is 3000r/min, centrifugation time is 20min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410085241.0A CN104892695A (en) | 2014-03-05 | 2014-03-05 | Preparation method for extracting tannin with camellia oleifera shells |
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| CN201410085241.0A CN104892695A (en) | 2014-03-05 | 2014-03-05 | Preparation method for extracting tannin with camellia oleifera shells |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106667833A (en) * | 2016-11-10 | 2017-05-17 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN111233554A (en) * | 2020-03-19 | 2020-06-05 | 广西壮族自治区林业科学研究院 | Organic fertilizer prepared by resource utilization of forestry processing residues and method thereof |
| CN112174977A (en) * | 2020-11-06 | 2021-01-05 | 浙江万里学院 | Method for extracting ellagic acid from oil tea fruit shell |
Citations (4)
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| JPS62106098A (en) * | 1985-11-01 | 1987-05-16 | Tsumura Juntendo Inc | Production of tannin |
| CN1149972A (en) * | 1995-11-13 | 1997-05-21 | 杨阗 | Natural multifunctional health tea beverage and producing process thereof |
| CN1450077A (en) * | 2003-04-22 | 2003-10-22 | 上海大学 | Method for extracting polygonum cuspidatum tannic acid |
| CN1872863A (en) * | 2006-06-23 | 2006-12-06 | 厦门大学 | Method for distilling tannin from plant |
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2014
- 2014-03-05 CN CN201410085241.0A patent/CN104892695A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| JPS62106098A (en) * | 1985-11-01 | 1987-05-16 | Tsumura Juntendo Inc | Production of tannin |
| CN1149972A (en) * | 1995-11-13 | 1997-05-21 | 杨阗 | Natural multifunctional health tea beverage and producing process thereof |
| CN1450077A (en) * | 2003-04-22 | 2003-10-22 | 上海大学 | Method for extracting polygonum cuspidatum tannic acid |
| CN1872863A (en) * | 2006-06-23 | 2006-12-06 | 厦门大学 | Method for distilling tannin from plant |
Non-Patent Citations (2)
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| 南京林学院树木提炼物工艺学教研组编: "《树木提炼物工艺学》", 31 July 1961 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106667833A (en) * | 2016-11-10 | 2017-05-17 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN106667833B (en) * | 2016-11-10 | 2019-08-09 | 湖南御家化妆品制造有限公司 | Camellia oleifera seed coat extract and preparation method and application thereof |
| CN111233554A (en) * | 2020-03-19 | 2020-06-05 | 广西壮族自治区林业科学研究院 | Organic fertilizer prepared by resource utilization of forestry processing residues and method thereof |
| CN112174977A (en) * | 2020-11-06 | 2021-01-05 | 浙江万里学院 | Method for extracting ellagic acid from oil tea fruit shell |
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