CN101412703B - Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid - Google Patents
Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid Download PDFInfo
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- CN101412703B CN101412703B CN2008102361441A CN200810236144A CN101412703B CN 101412703 B CN101412703 B CN 101412703B CN 2008102361441 A CN2008102361441 A CN 2008102361441A CN 200810236144 A CN200810236144 A CN 200810236144A CN 101412703 B CN101412703 B CN 101412703B
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Abstract
The invention relates to a complex extraction process for mulberry leaf flavonoid, polysaccharide and alkaloid through cogeneration, which is characterized by having the following extraction steps: raw material processing, extraction, and separation. The complex extraction process adopting a complex extraction method for the mulberry leaf flavonoid, the polysaccharide and the alkaloid obtained through the cogeneration has the advantages of mild condition, low energy consumption, small environment pollution; macroporous absorbent resin and chloroform used in the complex extraction process can be reused through regeneration; the economic value of the obtained effective ingredients is very high; the mulberry leaf resource is greatly saved; a new utilization approach of the agricultural product mulberry leaf is opened up; and the medicinal utilization value and the economic added value of the mulberry leaf are improved.
Description
One, technical field
The invention belongs to the bioseparation technology field, particularly a kind of coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique.
Two, background technology
Prior art: mulberry leaf are the dry leave of moraceae plants mulberry (Morus alba L.), cold in nature, sweet-bitter flavor, effect such as have dispelling wind and heat pathogens, clearing away lung-heat and moistening dryness, clear liver and improve vision, it is one of conventional Chinese medicine, be mainly used in diseases such as the heating due to the affection of exogenous wind-heat, dizzy headache, cough, swelling and pain in the throat, also be used for diseases such as the hot eyes due to the real heat of Liver Channel, puckery pain, many tears.Mulberry leaf nutrient is abundant, contains 16 seed amino acids of needed by human body, and 7 kinds of VITAMIN also contain nutritive elements such as calcium, iron, zinc, manganese, has that the property of medicine is gentle, characteristics such as have no side effect.Mulberry leaf medicinal with a long history in China is applied it very extensive as natural drug.Shennong's Herbal claims that mulberry leaf are " angle's leaf ", has nourishing YIN and supplementing blood, dispelling wind and heat pathogens, beneficial liver ventilation, the effect of antihypertensive diuretic.The Compendium of Material Medica record: mulberry leaf are the medicine of brothers Yang Ming, and juice is fried in shallow oil Dai Ming, can only quench one's thirst, and long hair makes eye bright.Japan's Zhibao Dan based on mulberry leaf in " Longevity and Life Preservation " " is obeyed not to climb up the longevity certainly " for a long time.
China is the origin center of mulberry tree, and the Morus plant has 4 mutation of 15 kinds, so the germplasm type of mulberry tree is rich and varied.All there is the mulberry tree distribution and cultivation in the most of area of China, and about 1,000 ten thousand mu of national mulberry field area is wherein maximum with main producing region output such as Jiangsu, Zhejiang, Sichuan.Mulberry leaf are mulberry tree biological yield the best parts, in actual silkworm and mulberry are produced, the situation of mulberry leaf surplus often occurs, and this causes the significant wastage of resource.If these resources are made full use of, its economic benefit and social benefit are all quite considerable.Modern pharmacological research is found, Folium Mori extract all has good curative effect at aspects such as disease such as treatment diabetes, hypertension, hyperlipidemia and anti-ageing, antiviral, anti-inflammatories, effective constituents such as flavones, polysaccharide and alkaloid in this and the mulberry leaf are closely related, and wherein the pharmacologically active of flavones in mulberry leaves is the most remarkable.Therefore, people have begun to be concerned about more and more the comprehensive utilization and the exploitation of medicinal ingredients resources such as flavones in mulberry leaves, polysaccharide and alkaloid in recent years.
At present, extraction of effective components has had some relevant patent reports in the relevant mulberry leaf, disclose as CN1683360 that to adopt the second alcohol and water respectively be extraction agent and eluent, utilize the method for macroporous adsorbent resin extraction separation flavones in mulberry leaves, CN1351085 discloses a kind of method of extracting the polysaccharides of Folium Mori component of molecular weight between 15000-50000 dalton from mulberry leaf, CN1338275 discloses the preparation method who extracts the total alkaloids of Folium Mori with alpha-glucosidase inhibitor activity from mulberry leaf, and CN1762428 discloses and utilized supercritical CO
2The method of the plain Alkaloid of the wild buttocks enzyme of extraction deoxidation and two kinds of effective constituents of flavones from mulberry leaf, CN1850166 discloses from mulberry leaf preparation polysaccharide and alkaloidal separation method and has prepared composition of white mulberry leaf blood-sugar-reducing effective components with this, and CN1589835 discloses Folium Mori extract of multiple bioactive ingredients such as containing flavones in mulberry leaves, mulberry leaf polyphenol, polysaccharides of Folium Mori, DNJ, GABA and uses thereof.Above patent is at single flavones or polysaccharide or alkaloid to mulberry leaf efficient part extraction process mostly, or two kinds of efficient parts wherein, do not see that with flavones in mulberry leaves, polysaccharide and alkaloid three be the composite extracting technique of target, do not realize coproduction of mulberry tea flavone, polysaccharide and three kinds of medicinal ingredientss of alkaloid, wasted valuable mulberry leaf resource greatly.
Though prior art shows the single active substances such as flavones, polysaccharide and alkaloid in the mulberry leaf relevant extraction process is arranged all, but not from fully utilizing the angle deep development mulberry leaf resource of medicinal ingredients resource, do not form the recombining process that a cover extracts typical case's three big active substances in the mulberry leaf, the flavones in the compound extraction mulberry leaf, polysaccharide and alkaloidal applied research are lacked basic data.Thereby exploitation coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique prepare corresponding extract, are suitable for suitability for industrialized production, for it has crucial meaning industrial tapping new markets such as medicine, health care, food, makeup.
Three, summary of the invention
Technical problem: the present invention is directed to above-mentioned technological gap, a kind of coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique are provided.This method goes for the extraction separation of three kinds of active principles in the multiple mulberry leaf resource, realizes the high-valued polycomponent comprehensive utilization of mulberry leaf resource.
Technical scheme: a kind of coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique, extraction step is:
A. raw material is handled: with new fresh mulberry leaf is raw material, and after oven dry became fragile, crushing screening got mulberry leaf powder;
B. extract: with mulberry leaf powder flooding after-filtration, get mulberry leaf residue and mulberry leaf water extract, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution extract the solution that obtains to contain Folium Mori alkaloid;
C. separate: after gained mulberry leaf water extract is adsorbed by macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, get the flavones in mulberry leaves ethanolic soln with ethanolic soln wash-out resin column again, alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing, can get the polysaccharides of Folium Mori powder, flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder, the Folium Mori alkaloid solution concentration is added hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate with ammoniacal liquor transfer pH to scope be 9～11, chloroform extraction, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder with the normal hexane washing.
The above mulberry leaf is white mulberry, Morus multicaulis or chicken mulberry.
In the above-mentioned flooding mulberry leaf powder process: extraction temperature is 60～90 ℃, and the pH scope of water is 7～10, and the solid-to-liquid ratio scope of mulberry leaf powder and water is 1:10～1:50.
Above-mentioned hydrochloric acid-ethanol-water solution extracts in the mulberry leaf residue process: the volume fraction scope of hydrochloric acid is 1%～3% in hydrochloric acid-ethanol-water solution, alcoholic acid volume fraction scope is 40%～80%, and the solid-to-liquid ratio scope of mulberry leaf residue and hydrochloric acid-ethanol-water solution is 1:10～1:50.
The content of total flavone scope of above-mentioned mulberry leaf water extract is that the content range of 2～8mg/mL, total polysaccharides is 2.5～10mg/mL.
In the above-mentioned hydrochloric acid soln leaching process: the volumetric concentration scope of hydrochloric acid is 0.3%～1%.
Above-mentioned macroporous adsorbent resin is crosslinked polystyrene macroporous adsorbent resin or crosslinked polypropylene macroporous adsorbent resin.
The volumetric concentration scope of the ethanolic soln that above-mentioned wash-out flavones in mulberry leaves is used is 50%～70%.
Above-mentioned chloroform extraction number of times scope is 1～10.
Above-mentioned normal hexane washing times scope is 1～3.
Beneficial effect:
(1) the present invention adopts the composite extracting method mild condition of coproduction gained flavones in mulberry leaves, polysaccharides of Folium Mori and Folium Mori alkaloid, the low pollution of energy consumption is little, used macroporous adsorbent resin and chloroform are reusable through regenerating, the active principle economic worth of gained is high, saved the mulberry leaf resource greatly, open up the new way of the utilization of these class agricultural byproducts of mulberry leaf, improved the medicinal utilization ratio and the economic value added of mulberry leaf resources.
(2) composite extracting technique of the present invention's employing adopts the buck formulation to extract flavones in mulberry leaves and polysaccharide, can obtain further to separate on the basis of flavones in mulberry leaves and polysaccharide Folium Mori alkaloid at the same time, and used extracting solution is a weak caustic solution, flavones in mulberry leaves and polysaccharide physico-chemical property there is not influence, and alkali aqueous solution has destroyed the fibrous tissue of vegetable cell, is beneficial to the extraction of later stage Folium Mori alkaloid.
(3) the present invention adopts resin adsorption method to separate flavones in mulberry leaves and polysaccharide, can remove a large amount of impurity simultaneously, and effectively separate two kinds of active principles, and the resin absorption partition method is simple to operate, is easy to industrial production and amplifies.
(4) the mulberry leaf residue with behind extraction flavones in mulberry leaves and the polysaccharide of the present invention's employing is a raw material, can effectively obtain Folium Mori alkaloid through extracting the method that obtains Folium Mori alkaloid once more, and foreign matter content is less in the gained alkaloid solution, and the medicinal extract after concentrating can improve the purity of Folium Mori alkaloid greatly after hydrochloric acid extracts once more.
(5) method of the chloroform extraction Folium Mori alkaloid of the present invention's employing can be finished removal of impurities and the operation of enrichment two portions simultaneously, and chloroform is bigger to the selectivity of total alkaloids of Folium Mori, through extraction repeatedly can come together fully in the mulberry leaf content lower but Folium Mori alkaloid active principles such as DNJ that economic worth is high significantly improve the economic worth of mulberry leaf.
Four, description of drawings
Fig. 1 coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique schema
Five, embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Selecting white Sang Sangye for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 60 ℃ of temperature, solid-to-liquid ratio 1:10, pH7 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 1%, ethanol 40% by solid-to-liquid ratio 1:10, volume fraction), extract the solution that obtains to contain Folium Mori alkaloid, flavones in mulberry leaves content is 2.5mg/mL in the gained mulberry leaf water extract, and polysaccharides of Folium Mori content is 2.0mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polystyrene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 50% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 0.3% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 9 with ammoniacal liquor, chloroform extraction 1 time, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 1 time with the normal hexane washing.Wherein, the flavones in mulberry leaves detection method: with the rutin is reference substance, adopts aluminum chloride development process spectrophotometer to measure at 510nm; The polysaccharides of Folium Mori detection method: with glucose is reference substance, adopts sulfuric acid phynol method spectrophotometer to measure at 490nm; The Folium Mori alkaloid detection method: with 1-S-GI (1-DNJ) is reference substance, adopts tetrabromo-mcresolsulfonphthalein staining spectrophotometer to measure at 415nm.Gained flavones in mulberry leaves purity is 75.21%, and polysaccharides of Folium Mori purity is 44.36%, and Folium Mori alkaloid purity is 32.98%.
Embodiment 2
Selecting the Morus multicaulis mulberry leaf for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 90 ℃ of temperature, solid-to-liquid ratio 1:50, pH 10 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 3%, ethanol 80% by solid-to-liquid ratio 1:50, volume fraction), extract the solution that obtains to contain Folium Mori alkaloid, flavones in mulberry leaves content is 3.2mg/mL in the gained mulberry leaf water extract, and polysaccharides of Folium Mori content is 2.8mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polystyrene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 70% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 1% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 11 with ammoniacal liquor, chloroform extraction 10 times, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 3 times with the normal hexane washing.Gained flavones in mulberry leaves purity is 73.37%, and polysaccharides of Folium Mori purity is 48.11%, and Folium Mori alkaloid purity is 38.48%.
Embodiment 3
Selecting chicken Sang Sangye for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 85 ℃ of temperature, solid-to-liquid ratio 1:40, pH 8 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 1.5%, ethanol 65% by solid-to-liquid ratio 1:30, volume fraction), extract the solution that obtains to contain total alkaloids of Folium Mori, total flavonoids of Folium Mori content is 2.8mg/mL in the gained mulberry leaf water extract, and mulberry leaf total polysaccharides content is 2.9mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polypropylene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 65% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 0.8% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 9 with ammoniacal liquor, chloroform extraction 8 times, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 2 times with the normal hexane washing.Gained flavones in mulberry leaves purity is 76.22%, and polysaccharides of Folium Mori purity is 44.41%, and Folium Mori alkaloid purity is 31.02%.
Embodiment 4
Selecting white Sang Sangye for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 70 ℃ of temperature, solid-to-liquid ratio 1:30, pH 9 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 2%, ethanol 75% by solid-to-liquid ratio 1:40, volume fraction), extract the solution that obtains to contain Folium Mori alkaloid, flavones in mulberry leaves content is 5.7mg/mL in the gained mulberry leaf water extract, and polysaccharides of Folium Mori content is 4.2mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polypropylene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 55% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 0.5% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 10 with ammoniacal liquor, chloroform extraction 6 times, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 1 time with the normal hexane washing.Gained flavones in mulberry leaves purity is 74.22%, and polysaccharides of Folium Mori purity is 56.91%, and Folium Mori alkaloid purity is 45.65%.
Embodiment 5
Selecting white Sang Sangye for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 80 ℃ of temperature, solid-to-liquid ratio 1:20, pH 10 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 2.5%, ethanol 70% by solid-to-liquid ratio 1:30, volume fraction), extract the solution that obtains to contain Folium Mori alkaloid, flavones in mulberry leaves content is 6.1mg/mL in the gained mulberry leaf water extract, and polysaccharides of Folium Mori content is 5.9mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polystyrene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 50% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 0.7% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 9.5 with ammoniacal liquor, chloroform extraction 9 times, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 3 times with the normal hexane washing.Gained flavones in mulberry leaves purity is 76.52%, and polysaccharides of Folium Mori purity is 58.74%, and Folium Mori alkaloid purity is 47.63%.
Embodiment 6
Selecting white Sang Sangye for use is raw material, and 60 ℃ of following oven dry are to becoming fragile after clean drying, and grinding and sieving (screen aperture 40～80 orders) obtains mulberry leaf powder.Mulberry leaf powder is obtained mulberry leaf water extract and mulberry leaf residue in the condition of 65 ℃ of temperature, solid-to-liquid ratio 1:40, pH 8 with the flooding after-filtration, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution (hydrochloric 2%, ethanol 70% by solid-to-liquid ratio 1:30, volume fraction), extract the solution that obtains to contain Folium Mori alkaloid, flavones in mulberry leaves content is 5.6mg/mL in the gained mulberry leaf water extract, and polysaccharides of Folium Mori content is 4.8mg/mL.After gained mulberry leaf water extract adsorbed by the crosslinked polypropylene macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, use 60% (volume fraction) ethanolic soln wash-out resin column to get the flavones in mulberry leaves ethanolic soln again.Alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing can get the polysaccharides of Folium Mori powder, and flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder.The Folium Mori alkaloid solution concentration is added 0.9% (volume fraction) hydrochloric acid soln to the medicinal extract fully to be extracted, filter back gained filtrate and transfer pH to 10 with ammoniacal liquor, chloroform extraction 6 times, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder 3 times with the normal hexane washing.Gained flavones in mulberry leaves purity is 77.79%, and polysaccharides of Folium Mori purity is 54.23%, and Folium Mori alkaloid purity is 42.46%.
Claims (6)
1. a coproduction of mulberry tea flavone, polysaccharide and alkaloidal composite extracting technique is characterized in that extraction step is:
A. raw material is handled: with new fresh mulberry leaf is raw material, and after oven dry became fragile, crushing screening got mulberry leaf powder;
B. extract: with mulberry leaf powder flooding after-filtration, get mulberry leaf residue and mulberry leaf water extract, the mulberry leaf residue is added hydrochloric acid-ethanol-water solution extract the solution that obtains to contain Folium Mori alkaloid; The volume fraction scope of hydrochloric acid is 1%～3% in hydrochloric acid-ethanol-water solution, alcoholic acid volume fraction scope is 40%～80%;
C. separate: after gained mulberry leaf water extract is adsorbed by macroporous adsorptive resins, extremely colourless with distilled water wash-out resin column earlier, obtain the polysaccharides of Folium Mori aqueous solution, get the flavones in mulberry leaves ethanolic soln with ethanolic soln wash-out resin column again, the volumetric concentration scope of the ethanolic soln that the wash-out flavones in mulberry leaves is used is 50%～70%, alcohol precipitation drying behind the gained polysaccharides of Folium Mori aqueous solution employing activated carbon decolorizing, can get the polysaccharides of Folium Mori powder, flavones in mulberry leaves ethanolic soln drying can get the flavones in mulberry leaves powder, the Folium Mori alkaloid solution concentration is added hydrochloric acid soln to the medicinal extract fully to be extracted, the volumetric concentration scope of hydrochloric acid is 0.3%～1%, filter back gained filtrate with ammoniacal liquor transfer pH to scope be 9～11, chloroform extraction, chloroform is removed in underpressure distillation, and dry back can get the Folium Mori alkaloid powder with the normal hexane washing.
2. coproduction of mulberry tea flavone according to claim 1, polysaccharide and alkaloidal composite extracting technique is characterized in that described mulberry leaf are white mulberry, Morus multicaulis or chicken mulberry.
3. coproduction of mulberry tea flavone according to claim 1, polysaccharide and alkaloidal composite extracting technique, the content of total flavone scope that it is characterized in that described mulberry leaf water extract are that the content range of 2～8mg/mL, total polysaccharides is 2.5～10mg/mL.
4. coproduction of mulberry tea flavone according to claim 1, polysaccharide and alkaloidal composite extracting technique is characterized in that described macroporous adsorbent resin is crosslinked polystyrene macroporous adsorbent resin or crosslinked polypropylene macroporous adsorbent resin.
5. coproduction of mulberry tea flavone according to claim 1, polysaccharide and alkaloidal composite extracting technique is characterized in that described chloroform extraction number of times scope is 1～10.
6. coproduction of mulberry tea flavone according to claim 1, polysaccharide and alkaloidal composite extracting technique is characterized in that described normal hexane washing times scope is 1～3.
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| CN101671294B (en) * | 2009-09-22 | 2011-12-21 | 广东太阳神集团有限公司 | Method for continuously extracting and separating 1-deoxynojirimycin (DNJ) and flavone from folium mori |
| CN101869605B (en) * | 2010-07-02 | 2012-05-02 | 安徽济人药业有限公司 | Method for extracting and separating mulberry leaf flavone and alkaloid composite |
| CN102327299A (en) * | 2011-07-26 | 2012-01-25 | 苏州宝泽堂医药科技有限公司 | Process for preparing daphniphyllum calycinum general flavones and total alkaloids simultaneously |
| CN102302557B (en) * | 2011-08-04 | 2014-03-19 | 广东省农业科学院蚕业与农产品加工研究所 | Continuous extraction method of natural alpha-glucosidase inhibitor |
| CN102726479B (en) * | 2012-04-20 | 2014-02-12 | 江苏大学 | Mulberry leaf flour with hyperglycemic effect and preparation method thereof |
| CN102964460A (en) * | 2012-11-16 | 2013-03-13 | 北京石油化工学院 | Method for continuously extracting and separating polysaccharides and 1-deoxynojirimycin from mulberry leaves |
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| CN103819572A (en) * | 2014-02-17 | 2014-05-28 | 王喜军 | Extraction technology for production of polysaccharide from mulberry leaf |
| CN104087421B (en) * | 2014-07-24 | 2016-05-25 | 中国烟草总公司郑州烟草研究院 | A kind of hollyhock flower extract co-production preparation method and application thereof |
| CN104940293B (en) * | 2015-06-15 | 2018-01-09 | 塔里木大学 | A kind of method that alkaloid, polysaccharide and flavones are extracted from mulberry leaf |
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| CN107721909B (en) * | 2017-11-02 | 2023-12-29 | 广西五和博澳药业有限公司 | Method and system for continuously extracting DNJ, flavone and polysaccharide from Moraceae plant |
| CN110393738B (en) * | 2019-08-27 | 2022-09-20 | 北京五和博澳药业股份有限公司 | Plant extraction method |
| CN111533680A (en) * | 2020-05-28 | 2020-08-14 | 成都医学院 | Folium Mori extract and folium Mori multicomponent mixture with blood sugar lowering effect prepared from the same |
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