CN103819572A - Extraction technology for production of polysaccharide from mulberry leaf - Google Patents

Extraction technology for production of polysaccharide from mulberry leaf Download PDF

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Publication number
CN103819572A
CN103819572A CN201410063375.2A CN201410063375A CN103819572A CN 103819572 A CN103819572 A CN 103819572A CN 201410063375 A CN201410063375 A CN 201410063375A CN 103819572 A CN103819572 A CN 103819572A
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polysaccharide
temperature
extraction
polysaccharides
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CN201410063375.2A
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王喜军
李海军
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Priority to CN201410063375.2A priority Critical patent/CN103819572A/en
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Abstract

The invention relates to an extraction technology for production of polysaccharide from mulberry leaf, especially to a method for extracting and separating high-purity polysaccharide from mulberry leaf. The invention adopts a method for removing impurities with a precipitating agent. The method is an extraction technology for production of polysaccharide from mulberry leaf, and the method has advantages of simple technology, high efficiency and high purity. By the adoption of the technology provided by the invention, a light-yellow high-purity mulberry-leaf polysaccharide product can be prepared at a time, and the purity can reach up to more than 95%. Production time occupied by the chromatogram process adopted for preparing a high-purity product by a conventional technology can be saved in quantity.

Description

The extraction process that a kind of polysaccharides of Folium Mori is produced
One, technical field
The present invention relates to the extraction process that a kind of polysaccharides of Folium Mori is produced, a kind of particularly method of extracting separating high-purity polysaccharides of Folium Mori from mulberry leaf.
Two, technical background
Mulberry leaf, it is the leaf of moraceae plants mulberry, mulberry tree has another name called a mulberry, Jing Sang, mulberry fruit tree, yellow wood leaf etc., whole nation most area has production more, has the effects such as anticoagulation, hypotensive, blood fat, decreasing cholesterol, antithrombotic formation and atherosclerosis, antiviral, antitumor, anti-ageing, antifatigue, spasmolysis, antiulcer agent, anti-inflammatory; Especially polysaccharides of Folium Mori, has effect significant hypoglycemic and that inhibition blood fat raises
Production technology and the technique of from mulberry leaf, extracting at present polysaccharides of Folium Mori are more, as: water extraction, ultrasonic extraction, microwave extraction, Enzymatic Extraction, the methods such as ultra-high voltage extraction are wherein the highest with ultrasonic extraction and microwave extraction yield, but the equal purity of the product of no matter taking which kind of extracting method gained is very low, product conventional content is conventionally in 20% left and right, and appearance luster is yellowish green, impure also many, can only serve as the mixed type pharmaceutical preparation of low side, not be suitable for the method for the opening application of high-end preparation; Therefore the content and the purity that improve product are particularly important.
Three, summary of the invention
The object of this invention is to provide the extraction process that a kind of polysaccharides of Folium Mori is produced, its technology is to adopt precipitation agent to go the technique of deimpurity method, is the extraction process that simple, efficient, the highly purified a kind of polysaccharides of Folium Mori of a kind of technique is produced.
The extraction process that a kind of polysaccharides of Folium Mori of the present invention is produced, is completed by following steps:
(1), by mulberry leaf raw material pulverizing to 1---5 order;
(2), add purifying waste water of 10 times of raw material weights, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times; United extraction liquid;
(3), add deployed precipitation agent to precipitate in extracting solution, the add-on of precipitation agent is extracting solution weight 5%, leaves standstill after 12 hours, and centrifugation is filtered and removed impurity, obtains filtered liquid;
(4), add the activated carbon powder of filtered liquid weight 2% in filtered liquid, at 60 ℃ of-75 ℃ of temperature, stir 30 minutes, after absorption soluble impurity, recentrifuge separating and filtering;
(5), by the filtered liquid after separating and filtering again, at 75 ℃ of-90 ℃ of temperature, when its weight 1/10 of vacuum concentration, add concentrated solution 5-10 doubly to measure high purity ethanol, carry out alcohol precipitation 12 hours;
(6), precipitation solution filtering separation, holds back solid part and with high purity ethanol washing, obtains polysaccharide goods;
(7), the polysaccharide vacuum dehydrating at lower temperature of gained, dried product is crushed to Powdered molecule;
(8), detect active constituent content, packing.
Described precipitation agent is by polymerize aluminum chloride 20%, polyacrylamide 5%, and gelatin 10%, calcium carbonate 5%, chitosan 60% forms, and after above raw material is merged and mixed, adds 4 times of pure water at 60 ℃ of temperature, to be stirred to completely and dissolves, and is statically placed under normal temperature for subsequent use.
Described high purity ethanol is the ethanol of 90--95%.
Technique provided by the invention can be accomplished disposable high-purity mulberry leaf polyose of producing the micro-Huang of color and luster, and purity, up to more than 95%, can be saved the shared production time of chromatography processes link that common process high-purity product adopts in a large number.
Four, embodiment:
Below in conjunction with embodiment, the present invention is done furtherly and carefully described.
Embodiment 1:
(1), take 100 grams of the mulberry leaf raw materials of pulverizing;
(2), add 10 times of raw material weight to purify waste water 1000 milliliters, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times, united extraction liquid;
(3), add 5 milliliters of deployed precipitation solvent to precipitate in extracting solution, leave standstill centrifugation after 12 hours and filter the solid impurity of removing absorption, obtain filtered liquid;
(4), filtered liquid adds the activated carbon powder of 2 grams recentrifuge separating and filtering after 30 minutes absorption soluble impurities of continuously stirring and pigment at 65 ℃ of temperature;
(5), filtered liquid is concentrated into 10 milliliters under 80 ℃ of vacuum conditions, adds the high purity ethanol of 50 milliliters of content 95% to carry out alcohol precipitation, the time is 12 hours;
(6), precipitation solution filtering separation, hold back the part of solid and with content 95% washing with alcohol to the colourless polysaccharide goods that obtain of washings;
(7), the polysaccharide goods vacuum dehydrating at lower temperature of gained, weigh 7.8 grams of gained after dry, colourity is slightly yellowish, reaches more than 95% through UV detection level;
(8), packing.

Claims (3)

1. the extraction process that polysaccharides of Folium Mori is produced, is characterized in that: its following steps complete:
(1), by mulberry leaf raw material pulverizing to 1---5 order;
(2), add purifying waste water of 10 times of raw material weights, under the temperature condition of 75 ℃, pump circulation is extracted 3 times continuously, each 3 hours extraction times; United extraction liquid;
(3), add deployed precipitation agent to precipitate in extracting solution, the add-on of precipitation agent is extracting solution weight 5%, leaves standstill after 12 hours, and centrifugation is filtered and removed impurity, obtains filtered liquid;
(4), add the activated carbon powder of filtered liquid weight 2% in filtered liquid, at 60 ℃ of-75 ℃ of temperature, stir 30 minutes, after absorption soluble impurity, recentrifuge separating and filtering;
(5), by the filtered liquid after separating and filtering again, at 75 ℃ of-90 ℃ of temperature, when its weight 1/10 of vacuum concentration, add concentrated solution 5-10 doubly to measure high purity ethanol, carry out alcohol precipitation 12 hours;
(6), precipitation solution filtering separation, holds back solid part and with high purity ethanol washing, obtains polysaccharide goods;
(7), the polysaccharide vacuum dehydrating at lower temperature of gained, dried product is crushed to Powdered molecule;
(8), detect active constituent content, packing.
2. the extraction process that a kind of polysaccharides of Folium Mori as claimed in claim 1 is produced, it is characterized in that: precipitation agent is by polymerize aluminum chloride 20%, polyacrylamide 5%, gelatin 10%, calcium carbonate 5%, chitosan 60% forms, and above raw material is merged after mixing, add 4 times of pure water at 60 ℃ of temperature, to be stirred to completely and dissolve, be statically placed under normal temperature for subsequent use.
3. the extraction process that a kind of polysaccharides of Folium Mori as claimed in claim 1 is produced, is characterized in that: the ethanol that high purity ethanol is 90--95%.
CN201410063375.2A 2014-02-17 2014-02-17 Extraction technology for production of polysaccharide from mulberry leaf Pending CN103819572A (en)

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Application Number Priority Date Filing Date Title
CN201410063375.2A CN103819572A (en) 2014-02-17 2014-02-17 Extraction technology for production of polysaccharide from mulberry leaf

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Application Number Priority Date Filing Date Title
CN201410063375.2A CN103819572A (en) 2014-02-17 2014-02-17 Extraction technology for production of polysaccharide from mulberry leaf

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CN103819572A true CN103819572A (en) 2014-05-28

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104403016A (en) * 2014-11-17 2015-03-11 安徽工程大学 Folium mori polysaccharide as well as separation and purification preparation method and application thereof
CN104829737A (en) * 2015-04-30 2015-08-12 华南理工大学 Crude raspberry leaf polysaccharide, and preparation method and application thereof
CN104877038A (en) * 2015-05-28 2015-09-02 北京石油化工学院 Method of extracting polysaccharide from mulberry leaves
CN105085700A (en) * 2015-08-25 2015-11-25 北京石油化工学院 Method for extracting, separating and purifying polysaccharide from mulberry leaves

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006160862A (en) * 2004-12-07 2006-06-22 Oki Bussan:Kk Method for extracting fucoidan
CN101121920A (en) * 2007-08-01 2008-02-13 陕西省科学院酶工程研究所 Preparation method for biological flocculant
CN101214279A (en) * 2008-01-09 2008-07-09 浙江工商大学 Method for extracting flavone and polysaccharide from mulberry leaf
CN101412703A (en) * 2008-11-17 2009-04-22 江苏科技大学 Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid
CN101709278A (en) * 2009-11-23 2010-05-19 浙江省环境保护科学设计研究院 Large-scaled culture method of high-concentration nitrosobacteria and application thereof
CN101712726A (en) * 2009-10-30 2010-05-26 浙江工业大学 Method for preparing high-purity mulberry leaf polysaccharide
CN102774947A (en) * 2012-07-25 2012-11-14 华南理工大学 Biological compound flocculant and application thereof
CN103524632A (en) * 2013-03-21 2014-01-22 王喜军 Rapid preparation process of astragalus polysaccharide

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006160862A (en) * 2004-12-07 2006-06-22 Oki Bussan:Kk Method for extracting fucoidan
CN101121920A (en) * 2007-08-01 2008-02-13 陕西省科学院酶工程研究所 Preparation method for biological flocculant
CN101214279A (en) * 2008-01-09 2008-07-09 浙江工商大学 Method for extracting flavone and polysaccharide from mulberry leaf
CN101412703A (en) * 2008-11-17 2009-04-22 江苏科技大学 Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid
CN101712726A (en) * 2009-10-30 2010-05-26 浙江工业大学 Method for preparing high-purity mulberry leaf polysaccharide
CN101709278A (en) * 2009-11-23 2010-05-19 浙江省环境保护科学设计研究院 Large-scaled culture method of high-concentration nitrosobacteria and application thereof
CN102774947A (en) * 2012-07-25 2012-11-14 华南理工大学 Biological compound flocculant and application thereof
CN103524632A (en) * 2013-03-21 2014-01-22 王喜军 Rapid preparation process of astragalus polysaccharide

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104403016A (en) * 2014-11-17 2015-03-11 安徽工程大学 Folium mori polysaccharide as well as separation and purification preparation method and application thereof
CN104403016B (en) * 2014-11-17 2016-09-07 安徽工程大学 A kind of mulberry leaf polysaccharide and preparation method for separating and purifying thereof and application
CN104829737A (en) * 2015-04-30 2015-08-12 华南理工大学 Crude raspberry leaf polysaccharide, and preparation method and application thereof
CN104829737B (en) * 2015-04-30 2017-09-29 华南理工大学 A kind of palmleaf raspberry leaf Thick many candies and preparation method and application
CN104877038A (en) * 2015-05-28 2015-09-02 北京石油化工学院 Method of extracting polysaccharide from mulberry leaves
CN105085700A (en) * 2015-08-25 2015-11-25 北京石油化工学院 Method for extracting, separating and purifying polysaccharide from mulberry leaves

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Application publication date: 20140528