CN109692248A - A kind of vine tea total flavone extracting method - Google Patents
A kind of vine tea total flavone extracting method Download PDFInfo
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- CN109692248A CN109692248A CN201710998006.6A CN201710998006A CN109692248A CN 109692248 A CN109692248 A CN 109692248A CN 201710998006 A CN201710998006 A CN 201710998006A CN 109692248 A CN109692248 A CN 109692248A
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- vine tea
- extracting method
- total flavone
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- tea total
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of vine tea total flavone extracting methods, using half bionical-biological enzyme extracting method, it overcomes conventional alcohol extracting method Extracting temperature height, be difficult to the disadvantages of separating-purifying, the addition of enzyme is conducive to leaching and the bioconversion of active material, under the premise of not destroying original ingredient, the general flavone in vine tea is more effectively extracted, the resulting vine tea general flavone extraction rate of the method for the present invention and content are higher.
Description
Technical field
The present invention relates to a kind of vine tea total flavone extracting methods, belong to pharmaceutical technology field.
Background technique
Vine tea, scientific name ampelopsis grossdentata belong to Vitaceae Ampelopsis, are a kind of Wild Lianes.Alias vine tea, rattan
Mother-in-law's tea, aobvious tea, Maoyanmei tea, manna tea, Radix Rhodiolae etc..Vine tea is distributed mainly on the south China Yangtze river basin, such as the Yunnan southeast
The ground such as portion, Fujian, Guangdong and Guangxi Provinces, Hunan, are commonly called as with many, and Hunan Zhangjiajie locality vine tea is commonly called as Tujia's mind tea, Radix Rhodiolae etc..?
Have specific record in the pharmacopeia such as " China book on Chinese herbal medicine ", " national Chinese herbal medicine compilation ", vine tea slightly sweet flavor is cool in nature, have it is clearing heat and detoxicating,
Drop heat promotes the production of body fluid, wind-damp dispelling, strengthening the bones and muscles, anti-inflammatory diuresis, and anti-arrhythmia resists myocardial ischemia, and alleviates alcohol function and other effects.
Flavonoid content is very high in vine tea, can exclude human body nocuousness acid eliminate with ketoboidies, remove blood in blood dirt,
Adjust blood lipid, reduce blood pressure, softening vessel effect it is significant, be adjustable reabsorption, improve sleep, pollakiuria etc..It can sterilization
It is anti-inflammatory, clearing heat and detoxicating, analgesia detumescence, blood fat-reducing blood pressure-decreasing, throat soothing cough-relieving, adjusting improve the immunity of the human body, to Staphylococcus aureus,
First, beta streptococcus, Diplococcus pneumopniae, Bacillus influenzae etc. have very strong killing ability.
Currently, the extracting method of flavones has hot water extraction method, Diluted Alcohol extraction method, acetone extraction, microwave loss mechanisms, surpasses
The shortcomings that filter method etc., these methods is time-consuming, labour-consuming and yield is lower.
Half bionical-biological enzyme extraction method is that first selection Suitable cellulases pre-process Chinese medicine, to destroy botanical medicine
Material cell wall.The method overcomes half biomimetic method, and Extracting temperature is high under normal pressure, is difficult to the disadvantages of separating-purifying, and the addition of enzyme is advantageous
Leaching and bioconversion in active material, are conducive to human body and active material are absorbed and utilized.
The present invention uses half bionical-biological enzyme extracting method, and overcoming half biomimetic method, Extracting temperature is high under normal pressure, is difficult to
The disadvantages of separating-purifying, the addition of enzyme is conducive to leaching and the bioconversion of active material, in the premise for not destroying original ingredient
Under, more effectively extract the general flavone in vine tea.
Summary of the invention
Abundant, reliable, stable vine tea total flavone extracting method is extracted the present invention provides a kind of.
For achieving the above object, a kind of vine tea total flavone extracting method of the present invention, the specific scheme is that
A kind of vine tea total flavone extracting method of the present invention, extracting method are as follows:
1. by the drying of vine tea cauline leaf, it crushed 20 ~ 40 meshes, it is spare.
2. a certain amount of powder is taken to be placed in flask, add 3 ~ 9 times of distilled water, salt acid for adjusting pH value (pH=1.0 ~ 3.0) impregnates 1
~ 2h reheats 0.5 ~ 2h of reflux, filtering；It is appropriate that biological enzyme is added into filter residue, 40 ~ 50 DEG C of 0.5 ~ 1h of hydrolysis, filtering；Again to
1 ~ 4 times of distilled water is added in filter residue, sodium hydroxide adjusts pH value (pH=6.0 ~ 8.0), is heated to reflux 0.5 ~ 2h, filters；Merge
Filtrate is drying to obtain.
A kind of vine tea total flavone extracting method of the present invention, step 2. the biological enzyme type be cellulase,
One of alpha-amylase, pectase, 1,4 beta-glucanase, lipase or multiple combinations.
A kind of vine tea total flavone extracting method of the present invention, the drying temperature are 60 ~ 70 DEG C.
The invention has the advantages that: the present invention to use half bionical-biological enzyme extracting method, overcomes half biomimetic method under normal pressure
Extracting temperature is high, is difficult to the disadvantages of separating-purifying, and the addition of enzyme is conducive to leaching and the bioconversion of active material, is not destroying
Under the premise of original ingredient, the general flavone in vine tea is more effectively extracted.
Specific embodiment
Following example is only to further illustrate the present invention, range that the invention is not limited in any way.
Embodiment 1
Vine tea powder is placed in flask, is added 4 times of distilled water (hydrochloric acid tune pH=1.0), 2h is impregnated, is heated to reflux 2h, is filtered；To
1% cellulase and 0.5% alpha-amylase, 40 DEG C of hydrolysis 1h, filtering are added in filter residue；4 times of (pH of distilled water are added into filter residue
=8.0) it, is heated to reflux 2.0h, is filtered；Merging filtrate is drying to obtain.
Embodiment 2
Vine tea powder is placed in flask, is added 5 times of distilled water (hydrochloric acid tune pH=1.5), 2h is impregnated, is heated to reflux 2h, is filtered；To
0.5% 1,4 beta-glucanase and 0.5% alpha-amylase, 45 DEG C of hydrolysis 2h, filtering are added in filter residue；Distilled water 3 is added into filter residue
Again (pH=7.0), it is heated to reflux 1.5h, filtered；Merging filtrate is drying to obtain.
Embodiment 3
Vine tea powder is placed in flask, is added 4 times of distilled water (hydrochloric acid tune pH=2.0), 2h is impregnated, is heated to reflux 1.5h, is filtered；
1.0% cellulase and 1.0% pectase, 40 DEG C of hydrolysis 1h, filtering are added into filter residue；4 times of distilled water are added into filter residue
(pH=8.0) are heated to reflux 2.0h, filtering；Merging filtrate is drying to obtain.
Embodiment 4
Using extraction rate % and general flavone content % as index, comparative example 1,2,3 and patent CN201510032579,
The effect of the extraction general flavone method of CN201611227557, CN201010148980.
Calculation formula:
Extraction rate %(g/g)=medicinal extract amount/crude drug amount × 100%
General flavone content %(g/g)=general flavone amount/crude drug amount × 100%
The result shows that the general flavone extraction rate and content of embodiment 1,2,3 are higher, as a result see the table below:
Claims (3)
1. a kind of vine tea total flavone extracting method, which is characterized in that its extracting method are as follows:
1) vine tea cauline leaf is dry, crushed 20 ~ 40 meshes, it is spare；
2) it taking a certain amount of powder to be placed in flask, adds 3 ~ 9 times of distilled water, salt acid for adjusting pH value (pH=1.0 ~ 3.0) impregnates 1 ~ 2h,
Reheat 0.5 ~ 2h of reflux, filtering；It is appropriate that biological enzyme is added into filter residue, 40 ~ 50 DEG C of 0.5 ~ 1h of hydrolysis, filtering；Again to filter residue
Middle to be added 1 ~ 4 times of distilled water, sodium hydroxide adjusts pH value (pH=7.0 ~ 8.5), is heated to reflux 0.5 ~ 2h, filters；Merge filter
Liquid is drying to obtain.
2. a kind of vine tea total flavone extracting method according to claim 1, which is characterized in that the step 2. biological enzyme
Type is one of cellulase, alpha-amylase, pectase, 1,4 beta-glucanase, lipase or multiple combinations.
3. a kind of vine tea total flavone extracting method according to claim 1, which is characterized in that the step 3. drying temperature
It is 60 ~ 70 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710998006.6A CN109692248A (en) | 2017-10-24 | 2017-10-24 | A kind of vine tea total flavone extracting method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710998006.6A CN109692248A (en) | 2017-10-24 | 2017-10-24 | A kind of vine tea total flavone extracting method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112390773A (en) * | 2020-12-02 | 2021-02-23 | 河南智盛优品生物科技有限公司 | Extraction process of vine tea flavone |
| CN114699468A (en) * | 2022-03-30 | 2022-07-05 | 湖北中烟工业有限责任公司 | Preparation method of vine tea extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105012372A (en) * | 2015-07-21 | 2015-11-04 | 西安交通大学 | Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones |
| CN106831454A (en) * | 2015-12-03 | 2017-06-13 | 康普药业股份有限公司 | A kind of ephedrine extraction method |
-
2017
- 2017-10-24 CN CN201710998006.6A patent/CN109692248A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105012372A (en) * | 2015-07-21 | 2015-11-04 | 西安交通大学 | Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones |
| CN106831454A (en) * | 2015-12-03 | 2017-06-13 | 康普药业股份有限公司 | A kind of ephedrine extraction method |
Non-Patent Citations (3)
| Title |
|---|
| 刘建兰等: "藤茶总黄酮和二氢杨梅素的提取检测研究进展", 《广东农业科学》 * |
| 薛璇玑等: "半仿生酶法提取柿叶中总黄酮的工艺筛选及优化", 《中国药房》 * |
| 金慧鸣等: "茅岩莓黄酮提取分离纯化技术的研究进展", 《食品研究与开发》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112390773A (en) * | 2020-12-02 | 2021-02-23 | 河南智盛优品生物科技有限公司 | Extraction process of vine tea flavone |
| CN114699468A (en) * | 2022-03-30 | 2022-07-05 | 湖北中烟工业有限责任公司 | Preparation method of vine tea extract |
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Application publication date: 20190430 |