CN113633703A - Hypoglycemic application of tea extract - Google Patents
Hypoglycemic application of tea extract Download PDFInfo
- Publication number
- CN113633703A CN113633703A CN202111085451.6A CN202111085451A CN113633703A CN 113633703 A CN113633703 A CN 113633703A CN 202111085451 A CN202111085451 A CN 202111085451A CN 113633703 A CN113633703 A CN 113633703A
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- CN
- China
- Prior art keywords
- camellia oleifera
- leaf extract
- extract
- leaves
- oil tea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The invention discloses an application of a camellia oleifera leaf extract in reducing blood sugar, wherein the camellia oleifera leaf extract is used as an active ingredient of a composition or a mixture for inhibiting glycogen metabolism of a human body. The invention has the beneficial effects that the camellia oleifera leaf extract has good use effect in the aspect of reducing blood sugar of human bodies, thereby providing a new research direction and a great trend commercialization path for camellia oleifera leaves, exploring the application of the camellia oleifera leaf extract in the fields of related foods, health products and medicines, and exploring the application of the camellia oleifera leaf extract as a functional ingredient in the daily chemical fields of washing, skin care and the like, thereby expanding the research application range of camellia oleifera leaves.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a hypoglycemic application of a camellia oleifera leaf extract.
Background
Research shows that alpha-glucosidase is one of important members of carbohydrate metabolism in organisms and directly participates in the metabolism pathways of starch and glycogen. The alpha-glucosidase inhibitor has wide application space in the field of relieving and treating diabetes, can inhibit the activity of alpha-glucosidase, can play a role in regulating the blood sugar level by reducing the postprandial blood sugar peak value, is favorable for controlling the development of diabetes, particularly the occurrence of complications.
The camellia oleifera is a special high-grade natural oil material in China growing in mountains and hilly lands in subtropical regions in the south of China, is a evergreen small arbor in the camellia family, is one of four major woody oil materials, is used for extracting tea oil because camellia seeds are the part with the highest oil content, and is applied to many aspects, and meanwhile, the tea meal after the tea oil is extracted contains 20-25% of tea saponin, 18-20% of polysaccharide and 15-20% of protein and is also used as a raw material for secondary recycling. At present, the research and the industrial application of extracting tea saponin from the camellia seed and camellia seed cake are mature, and the single tea saponin has no function report of inhibiting alpha-glucosidase.
However, the application and research of the extract of the oil tea leaves are relatively few, on one hand, the oil tea leaves are used as a part of the oil tea tree and are not economic raw materials, and the oil tea trees cannot be harvested under normal conditions, on the other hand, the oil tea is an obvious young and old crop, the yield of the oil tea fruits is low in the young period, redundant branches and leaves need to be trimmed, a large number of old leaves are generated, and the oil tea tree is maintenance cost for the operation of an oil tea garden and cannot directly create economic value.
The invention application with the application number of CN202011473896.7 discloses a camellia oleifera cake extract and a preparation method and application thereof, and the camellia oleifera cake extract is mainly prepared by adding water into camellia oleifera cake, centrifuging to obtain supernatant, adding ethanol, standing for precipitation, and centrifuging for the second time. Aims to remove the tea saponin, overcomes the defect that the traditional water extraction and alcohol precipitation product contains the tea saponin, and the finished product is applied to the feed.
The invention application with the application number of CN201210566628.9 discloses a mild hand sanitizer containing a tea seed meal extract, which mainly utilizes tea saponin in tea seed meal as a natural surfactant to be applied to bacteriostasis and disinsection, and also discloses the technical characteristics that the washed skin can be more moistened due to the moisturizing effect of tea seed polysaccharide, and the anti-oxidation effect of the polysaccharide and the nutritional effect of polypeptide can increase the activity of cells.
The invention application with the application number of CN202110391266.3 discloses an application of a tea-oil tree leaf extract in inhibiting sebum secretion, and mainly discloses that the tea-oil tree leaf extract is used for inhibiting the sebum secretion of sebaceous glands, so that the tea-oil tree leaf can be used as an effective industrial raw material to be applied to related foods, health-care products and medicines, and the possibility of being used as a functional ingredient in daily chemical fields of washing and caring skin and the like is increased.
The invention application with the application number of CN2011102027611 discloses a new application of oil-tea camellia cake polysaccharide, and the obtained oil-tea camellia cake polysaccharide is subjected to in vitro activity test, so that the extracted and purified (concentrated) oil-tea camellia cake polysaccharide has a good effect of inhibiting the activity of alpha-glucosidase.
Disclosure of Invention
Aiming at the technical defects in the background art, the invention provides the hypoglycemic application of the camellia oleifera leaf extract, solves the technical problems and meets the actual requirements, and the specific technical scheme is as follows:
use of an extract of camellia oleifera leaves as an active ingredient of a composition or mixture for inhibiting glycogen metabolism in a human body for lowering blood glucose.
As a further technical scheme of the invention, the camellia oleifera leaf extract is used as an active ingredient of a medicine, a health product, a food or a washing and caring daily chemical product for inhibiting glycogen metabolism of a human body.
As a further technical scheme of the invention, the camellia oleifera leaf extract is used as an active ingredient for inhibiting the activity of alpha-glucosidase in medicaments, health products, foods or washing and caring daily chemical products for inhibiting glycogen metabolism of human bodies.
As a further technical scheme of the invention, the camellia oleifera leaf extract is extracted by the following steps: removing pest and pathological part of the selected oil tea leaves with complete leaves, removing leaf stalks, crushing, taking fine powder through a screen with the mesh number not less than 50 meshes, drying, weighing, and storing at 0-4 ℃ for later use; weighing an ethanol solution with a volume not less than 30 times of that of water equivalent to the weight of the camellia oleifera leaf powder, mixing, performing ultrasonic extraction, and centrifuging the extract to obtain a supernatant, namely the camellia oleifera leaf extract; and drying the extracting solution until no water exists and only solid residues exist, namely the camellia oleifera leaf extract.
As a further technical scheme of the invention, the camellia oleifera leaf extracting solution is diluted by distilled water until the concentration of the camellia oleifera leaf powder is not more than 5x 10-5g/L, is used for testing the inhibition rate of the activity of the alpha-glucosidase.
The invention has the beneficial effects that: the camellia oleifera leaf extract has a remarkable inhibition effect on alpha-glucosidase, has a better blood sugar reducing capability than the mulberry leaf extract and acarbose which are proved in the existing research, and has a better inhibition capability even in a diluted state.
More importantly, the camellia oleifera leaf extract obtained by the extraction process provides a new research direction and a great trend commercialization path for camellia oleifera leaves, converts camellia oleifera leaves which can be picked all the year round and have almost zero application value into industrial raw materials with good application value, especially for old leaves, finds a way for reasonably utilizing the wastes, enables the wastes to serve as active ingredients of hypoglycemic compounds, explores the application of the wastes in the fields of related foods, health products and medicines, and serves as functional ingredients in the daily chemical fields of washing, skin care and the like, and accordingly widens the research application range of the camellia oleifera leaves.
Drawings
FIG. 1 is a graph of the results of the inhibition of alpha-glucosidase by Camellia oleifera leaf extract at various concentrations.
FIG. 2 is a free amino acid content profile of Camellia oleifera leaf extract.
Detailed Description
Embodiments of the present invention will be described below with reference to the accompanying drawings and related embodiments, which are not limited to the following embodiments, and the present invention relates to relevant necessary components in the technical field, and should be regarded as known in the art and known to those skilled in the art and understood by the technical field.
In the invention, the extraction process of the camellia oleifera leaf extract can be implemented according to the following steps: removing pest and pathological part of the selected oil tea leaves with complete leaves, removing leaf stalks, crushing, taking fine powder through a screen with the mesh number not less than 50 meshes, drying, weighing, and storing at 4 ℃ for later use; weighing a certain amount of oil tea powder, mixing with 70% ethanol solution with the volume 30 times that of the oil tea powder and the like, performing ultrasonic extraction for 4 hours, and centrifuging the extract to obtain supernatant, namely the oil tea leaf extract; and drying the extracting solution until no water exists and only solid residues exist, namely the camellia oleifera leaf extract.
In order to verify the utilization value of the oil tea leaves, most of the samples of the oil tea leaves taken by the invention are old leaves which are 5 years old or more and have reached the high yield stage and 6 months old or more.
Wherein the rotation speed during centrifugation is not less than 4000 r/min, the centrifugation time is not less than 10 min, and the solid content of the camellia oleifera leaf extract measured by a drying method is 10 mg/mL.
In human body, alpha-glucosidase is one of important members in the sugar metabolism path in organism, directly participates in the metabolism path of starch and glycogen, and can play a role in regulating blood sugar level by reducing postprandial blood sugar peak value by inhibiting the activity of alpha-glucosidase50A value of 0.080 mg/mL, as a positive control, IC of acarbose500.110 mg/mL, and it is generally considered that IC50The smaller the value of (A), the stronger the specific property of the antibody, and the better the inhibitory effect.
Combining with the in vitro study on the inhibitory activity of different extraction parts of mulberry leaves on alpha-glucosidase from the section of volume 6 of volume 38 of 2015, the statement of university of Xinjiang medical science, the preparation of the mulberry leaf extract preferably adopts the total water extract which is formed by soaking a certain amount of mulberry leaves in hot water and then filtering and refluxing the soaked mulberry leaves for 2 times and has the best inhibitory effect on the alpha-glucosidase, thereby showing that the mulberry leaf extract has the best inhibitory effect on the alpha-glucosidaseThe extract should have a specific gravity of the active ingredient lighter than that of water. In the research on the in vitro blood sugar lowering and antioxidant effects of the folium Mori DNJ extract disclosed in 2017, volume 42, volume 8, it is shown that, according to the determination, folium Mori DNJ is effective alpha-glucosidase inhibitory active component, and is purified compound, IC thereof50Is 0.350 mg/mL, but is worse than the total water extract, so the mulberry leaf extract has better using effect under the water-based environment.
Therefore, according to the above situation, the inhibition effect of the camellia oleifera leaf extract solution prepared in the invention on the alpha-glucosidase activity is detected, the efficacy of the camellia oleifera leaf extract in reducing blood sugar can be judged compared with the existing method, and the degree of the effect of the camellia oleifera leaf extract in reducing blood sugar, especially the alpha-glucosidase inhibition rate can be judged by comparing the existing optimal camellia oleifera leaf extract with acarbose serving as a reference. Currently, enzyme activity determination methods are commonly used to test inhibition rates.
The reaction principle is as follows:
p-nitrophenyl-beta-D-glucopyranoside (PNPG) is used as a substrate, alpha-glucosidase can specifically hydrolyze glycosidic bonds in the PNPG, the generated hydrolysate p-nitrophenol (PNP) has a light absorption value at 405 nm, and the content of the hydrolysate PNP is calculated by measuring the light absorption value, so that the alpha-glucosidase inhibition activity of the sample extract is calculated.
The reaction system is as follows:
respectively sucking 20 mu L of 0.1 mol/L phosphate buffer solution (pH 6.8) and 20 mu L of sample solution to be detected, adding 40 mu L of each of 2.0 mmol/L PNPG and 0.1U/mL alpha-glucosidase, shaking up, keeping the temperature at 37 ℃ for reaction for 20 min, adding 80 mu L of 0.2 mol/L sodium carbonate solution to stop the reaction, and measuring the absorbance at 405 nm.
And (3) replacing enzyme liquid with PBS as blank control, repeating for 3 times, taking an average value, and calculating the activity of the alpha-glucosidase according to the content change of PNP generated by the reaction. The final reaction volume was determined to be 200. mu.L using a 96-well plate as a reaction carrier.
Since all the extracts of the samples contained the pigment, the inhibition ratio of the α -glucosidase activity was calculated as follows:
inhibition (%) = (a)m-Ay）/Am×100；
In the formula: a. themAbsorbance values after reaction of enzyme with substrate (minus the corresponding blank);
Aythe absorbance of the enzyme reaction after addition of inhibitor (minus the corresponding blank) was used.
Example 1: weighing and quantifying the prepared solid matters of the camellia oleifera leaf extract, and preparing a camellia oleifera leaf extract solution with the concentration of 0.040 mg/mL.
Example 2: weighing and quantifying the prepared solid matters of the camellia oleifera leaf extract, and preparing a camellia oleifera leaf extract solution with the concentration of 0.078 mg/mL.
Example 3: weighing and quantifying the prepared solid matters of the camellia oleifera leaf extract, and preparing a camellia oleifera leaf extract solution with the concentration of 0.156 mg/mL.
Example 4: weighing and quantifying the prepared solid matters of the camellia oleifera leaf extract, and preparing a camellia oleifera leaf extract solution with the concentration of 0.312 mg/mL.
Example 5: weighing and quantifying the prepared solid matters of the camellia oleifera leaf extract, and preparing a camellia oleifera leaf extract solution with the concentration of 4.200 mg/mL.
Among them, the observed inhibition ratio of the sample of example 1 was 28.72%, the observed inhibition ratio of the sample of example 2 was 51.68%, the observed inhibition ratio of the sample of example 3 was 84.34%, the observed inhibition ratio of the sample of example 4 was 87.68%, and the observed inhibition ratio of the sample of example 5 was 86.48%.
Among them, the actual inhibition ratios of the first 4 examples can be measured according to data analysis, and the inhibition curves are y = -16.958x2+ 8.5285x-0.0308 (R = 0.9978), wherein x is the concentration of camellia oleifera leaf extract, y is the inhibition ratio of the sample on α -glucosidase, and the specific curve can be seen with reference to fig. 1.
Referring to fig. 1, it can be seen that after a certain concentration is exceeded, the inhibition rate of the camellia oleifera leaf extract on alpha-glucosidase is obviously increased until the peak value falls, and it is determined that an optimal concentration value exists at the concentration of the camellia oleifera leaf extract, so that the inhibition rate of all effective components in the camellia oleifera leaf extract is the highest, and at the concentration, other compounds such as polysaccharides and polyphenols exert effects synergistically.
By calculating the above test values, the IC of the tea extract can be determined according to the calculation50The value of (A) is 0.073mg/mL, which is obviously smaller than the IC of acarbose which is widely used in medicine for blood sugar inhibition50Value, IC of mulberry leaf extract which is also less than the current best hypoglycemic component50It is much better than the tea seed cake extract, and thus shows a very strong inhibitory effect.
More importantly, in the invention, the preparation of the camellia oleifera leaf extract is based on the dilution of the solid concentration, but the better blood sugar reducing effect is reflected, and the dilution of the extract concentration can reduce the ratio of polysaccharide in the extract, so that the camellia oleifera leaf extract cannot be purely used as a polysaccharide component but is a result of the combined action of the polysaccharide component, the polyphenol component and other components, on the premise of being based on natural raw materials, and meanwhile, the camellia oleifera leaf extract is also used as a raw material with more active ingredients. However, in the prior art, the research on the tea seed meal extract is based on the inhibition of polysaccharide components in the tea seed meal on alpha-glucosidase, and the effect is poor, and meanwhile, the published contents prove that the flavone and tea saponin components of new leaves are more than those of old leaves, but even if the flavone and tea saponin components are more than those of the old leaves, the utilization of the tea seed meal leaves is still in the analysis stage of single components, and the method cannot provide obvious guidance for developing and utilizing the industrial value of the tea leaves.
Therefore, compared with the concentrated tea seed cake extract and the simple analysis of the components of the tea-oil tree leaves, the tea-oil tree leaf extract provided by the invention adopts different ideas, and achieves a better effect.
In the existing oil-tea camellia industry, oil-tea camellia fruits, oil-tea camellia seeds, oil-extracted tea dregs and oil-tea camellia seed oil are key points of current research and production, are the most important value creation products in the whole industry chain, and basically reflect the fact in the background technology. For the camellia oleifera, the branch and leaf parts of camellia oleifera are often the most neglected, and no matter general fruit growers, camellia oleifera garden operators or even raw material purchasers, the camellia oleifera leaves do not have any high value factor, especially for a plurality of leaves growing in the middle of the young, countless camellia oleifera leaves are converted from old leaves into waste after falling off or being trimmed, and then are converted into humus soil to be recycled and reused in the cheapest way, so that countless camellia oleifera branch and leaves do not enter the camellia oleifera industrial chain to be greatly wasted, and moreover, as the product after the camellia oleifera fruits are hulled, camellia oleifera cattail is burned off to be used as fertilizer in most cases or is used for extracting some special raw materials.
The application mode of the invention is provided, so that the utilization value of the oil tea is fully reflected: firstly, the camellia oleifera leaves have the characteristic of being picked all the year round, in the production process of camellia oleifera fruits, not only can the falling of camellia oleifera leaves continuously occur, but also redundant camellia oleifera leaves trimmed according to the planting requirement continuously exist, particularly, camellia oleifera trees are annual crops with obvious sizes, the yield of the camellia oleifera fruits is limited by the land condition, the annual harvest cannot be realized, the picking of the camellia oleifera leaves in the small year has no influence on the harvest of the camellia oleifera fruits, so that the sources of camellia oleifera branches and leaves are far more stable than that of the camellia oleifera fruits, and the camellia oleifera branches and leaves have extremely good advantages as raw materials required by industrial production; furthermore, the extraction mode of the camellia oleifera leaf extract is close to that of the existing mulberry leaf extract, so that the extraction difficulty is extremely low, further, the camellia oleifera leaf extract can be used as a raw material in a diluent form without refining and refining after being extracted, the corresponding alpha-glucosidase inhibition effect is exerted, the dilution and utilization form is completely consistent with the actual production scene, the application conversion efficiency of the camellia oleifera leaf extract is improved, and the camellia oleifera leaf extract is also suitable for mass production and use and is easy to store.
The alpha-glucosidase inhibitor has wide application space in the field of relieving and treating diabetes, so that the camellia oleifera leaf extract can be used as an active component for inhibiting alpha-glucosidase in medicines, foods or health care products.
While numerous studies have shown that the products of the "glycation end products" (AGEs), which affect collagen function in the Skin, are indeed related to Skin Aging, they are also present in human activities and are involved in the development of various diseases, such as diabetic complications, and in recent years, they have been found to be related to Skin Aging (Skin Aging), although they are used to describe "browning during food heating", i.e., "maillard reaction".
In brief, the principle is that carbohydrate molecules are combined with protein or lipid molecules without enzyme action to generate AGEs, which causes adverse effects on human body including skin.
Therefore, the inhibition of the camellia oleifera leaf extract on the activity of the alpha-glucosidase can obviously improve the adverse state of blood sugar in a human body, and adjust the blood sugar to a healthier low level, so that the camellia oleifera leaf extract can also participate in the inhibition of the saccharification reaction of the skin for external use, thereby delaying the aging of the skin, and the adverse current situation of the skin such as alopecia, skin chap and the like of a user can be obviously improved by adding the camellia oleifera leaf extract into washing, protecting and daily chemical products such as shower gel, facial cleanser, shampoo and even toothpaste.
The content disclosed by the invention is logically that organic components are inevitably distributed in different parts of a plant in different ways, so that the research on the camellia oleifera seed cake in the prior art cannot be directly applied to the research on the camellia oleifera leaves, the research on the camellia oleifera seed cake lies in the extraction and utilization of tea saponin, the effect of the tea saponin is generally used for cleaning, nursing and skin care, but the tea saponin cannot be used as the inhibition of the activity of alpha-glucosidase, and the research in the prior art cannot reflect the effect of the camellia oleifera leaves in the aspect of reducing the blood sugar.
The camellia oleifera is widely planted in China, many farms exist, and the yield of tea leaves is high, so that the camellia oleifera has a very good application prospect, has a good degree of inhibition effect on the activity of alpha-glucosidase, and can intuitively and accurately reflect the postprandial blood sugar level of a user.
In order to better research the oil tea, the invention also detects the effective components of the oil tea, and finds that the moisture content in the oil tea is 6.62 +/-0.16 percent and the protein content is 6.99 +/-0.24 percent. The low water content is beneficial to the preparation of the camellia oleifera leaf extract and the storage and transportation of the camellia oleifera leaves, and the protein is proved to have the effects of stimulating the growth of new hair, delaying the hair damage process and improving the conditions of the hair and the scalp, so that the certain protein content is beneficial to reducing the skin aging problem caused by saccharification.
Referring to fig. 2, proteins are macromolecules composed of amino acids and are not easily absorbed and utilized by skin, and hydrolysis products of the proteins, namely small molecular peptides and amino acids, are the tendency of the proteins to be applied to daily chemical products. Through measurement and calculation, the tea-oil tree leaves contain 7 essential amino acids such as Threonine (THR), Valine (VAL), Methionine (MRT), Isoleucine (ILE), Leucine (LEU), Lysine (LYS) and Histidine (HIS), the contents of the essential amino acids are 17.632, 3.262, 0.156, 3.102, 3.332, 4.389 and 2.480 respectively, and the above units are mg/100 g.
Wherein, the threonine structure contains hydroxyl, has a water-holding effect on human skin, is combined with oligosaccharide chain, and plays an important role in protecting cell membranes. Aspartic acid has antioxidant effect, and can relieve oxidation toxicity of dermal fibroblast caused by increase of free radical generation source. Glutamic acid has the effects of protecting skin barrier function and promoting functional recovery thereof. Methionine can enhance tissue metabolism and anti-inflammatory ability, and can be used for skin care products for conditioning, anti-aging or preventing and treating acne; has effects in regulating and protecting hair, and promoting hair growth. Cystine has effects of preventing skin allergy, treating wound, and preventing and treating eczema.
The oil tea also contains 0.90 + -0.00% of polysaccharide, 2.61 + -0.04% of flavone, and 8.35 + -0.26% of polyphenol.
In addition, researches report that the polysaccharide can repair skin tissues by activating cell growth factors, and meanwhile, the polysaccharide has good affinity with human skin and can be widely applied to cosmetics as a functional ingredient.
Flavone compounds are the main active substances in plants, and the content thereof is related to the species, source and even season of leaf picking of the plants. The camellia oleifera leaves can be picked in four seasons, so that the content of flavone can be kept stable, and the flavone has good antioxidant activity and good medical and cosmetic values.
The high polyphenol content indicates that the biological activity is strong. A large number of research results show that the plant polyphenol has strong antioxidation, obvious effects of bacteriostasis, cancer resistance, ageing resistance, cholesterol increase inhibition and the like, and the disease can be effectively prevented and inhibited by taking a certain amount of plant polyphenol.
In addition, published research data indicate that the content of tea saponin in camellia oleifera leaves is not high and is much lower than that in camellia fruits. And under the influence of the age of the tree and the age of the leaves, the tea saponin content of the oil tea leaves with the age of more than 5 years is gradually reduced, and the tea saponin content of the oil tea leaves is higher as the age of the leaves is larger. However, since the picking of the camellia oleifera fruit is also affected by seasons, the act of harvesting in advance for picking up the camellia oleifera fruit is not beneficial to the ripening of the camellia oleifera fruit, and therefore, fresh camellia oleifera leaves are not usually specially received for collecting the tea saponin. Therefore, the research on the content of tea saponin in the application of reducing the blood sugar by using the oil-tea camellia meal does not have continuity and reference on the research on the blood sugar of the oil-tea camellia leaf extract, which is also a main factor generally ignored by the existing industry for the oil-tea camellia leaves.
Table 1: content of metal elements in branches and leaves of camellia oleifera
The table above shows the content of metal elements in the camellia oleifera leaves after measurement, wherein the content of lead, arsenic, mercury and cadmium in the camellia oleifera leaves is far lower than the limit value in technical safety specifications for cosmetics (2015 edition), and the content of heavy metal elements simultaneously meets the recommended requirements of CIR on plant raw materials in the literature, so that the camellia oleifera leaves as industrial raw materials for mass production meet safe use, are rich in trace elements necessary for human bodies such as iron, copper, zinc, selenium and manganese, and have more contents and important elements:
1) the content of potassium and sodium is the most, the potassium and sodium account for the main part of the extract of the camellia oleifera leaves and are important for regulating the osmotic pressure balance of the body fluid circulation of a human body.
2) Magnesium as an activator of various enzymes can participate in more than 300 enzymatic reactions in human bodies, and plays an important role in the normal operation of human body functions.
3) Manganese is a component of Mn-SOD, can remove peroxide, enhance organism immunity, delay skin aging, and reduce wrinkles.
4) Calcium affects the health of bones and cardiovascular system of human body, and calcium is one of the most obvious elements which can be quickly lost from human body with the increase of human age.
5) Iron is involved in the activity of various enzymes in the body, and iron deficiency can cause anemia, affect nutrient supply, and cause skin to be more susceptible to aging.
6) Zinc is widely involved in the composition of various enzymes in the body, and zinc has the effects of resisting oxidation and inhibiting the activity of the tyrosine kinase.
7) Selenium has effects of resisting oxidation and enhancing immunity, and can be combined with various heavy metals such as mercury, lead, cadmium, etc. in vivo to form metal selenium protein compound for removing toxic substance and removing toxic substance. Research shows that selenium is closely related to the occurrence and development of certain skin diseases, and oral or local external selenium or compounds thereof have prevention and treatment effects on the skin diseases.
In addition, although the components in the camellia oleifera leaves are influenced by the leaf age factors, the content of the components is changed, but the influence of the leaf age on the active ingredients of the extract is negligible for preparing the hypoglycemic extract.
According to the invention, the inhibition effect of the camellia oleifera leaf extract on alpha-glucosidase is very remarkable, the inhibition effect of the camellia oleifera leaf extract on alpha-glucosidase is better than that of a mulberry leaf extract and acarbose which are proved in the existing research, the inhibition effect of the camellia oleifera leaf on alpha-glucosidase is basically judged by combining the analysis of the components, the inhibition effect of the camellia oleifera leaf on alpha-glucosidase is obtained based on the synergistic action of a plurality of components such as polysaccharide, polyphenol, flavone and amino acid, the study fact of the influence on alpha-glucosidase is met, although the specific components are extracted independently in the study stage, the application of the camellia oleifera leaf extract on glucose reduction has bright application scenes and specific application fields.
The camellia oleifera leaf extract obtained by the extraction process provides a new research direction and a great trend commercialization path for camellia oleifera leaves, especially finds a more effective development and utilization path with good economic prospects for the camellia oleifera leaves with the largest yield in the whole field of the camellia oleifera industry, explores the application of the camellia oleifera leaf extract in the fields of related foods, health products and medicines, and also finds the application of the camellia oleifera leaf extract as a functional ingredient in the daily chemical fields of washing, skin care and the like, thereby expanding the research and application range of the camellia oleifera leaves.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. Use of an oil tea extract for lowering blood sugar, characterized in that the oil tea extract is used as an active ingredient of a composition or mixture for inhibiting glycogen metabolism in a human body.
2. The use according to claim 1, wherein the oil tea leaf extract is used as an active ingredient of a drug, health product, food or cleaning and caring daily chemical for inhibiting glycogen metabolism in a human body.
3. The use according to claim 2, wherein the oil tea leaf extract is used as an active ingredient for inhibiting the activity of α -glucosidase in a human glycogen metabolism-inhibiting drug, health product, food or daily use chemical.
4. Use according to any one of claims 1 to 3, wherein the oil tea leaf extract is extracted by: removing pest and pathological part of the selected oil tea leaves with complete leaves, removing leaf stalks, crushing, taking fine powder through a screen with the mesh number not less than 50 meshes, drying, weighing, and storing at 0-4 ℃ for later use; weighing an ethanol solution with a volume not less than 30 times of that of water equivalent to the weight of the camellia oleifera leaf powder, mixing, performing ultrasonic extraction, and centrifuging the extract to obtain a supernatant, namely the camellia oleifera leaf extract; and drying the extracting solution until no water exists and only solid residues exist, namely the camellia oleifera leaf extract.
5. The use according to claim 4, wherein the oil tea leaf extract is diluted with distilled water to a concentration of the oil tea leaf powder of not more than 5x 10-5g/L, is used for testing the inhibition rate of the activity of the alpha-glucosidase.
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Citations (3)
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| CN102885847A (en) * | 2011-07-19 | 2013-01-23 | 中南林业科技大学 | Novel uses of camellia oleifera cake polysaccharide |
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| CN105924484A (en) * | 2016-04-29 | 2016-09-07 | 中南林业科技大学 | Method for extracting quercetin derivatives from camellia oleifera leaves |
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| CN102885847A (en) * | 2011-07-19 | 2013-01-23 | 中南林业科技大学 | Novel uses of camellia oleifera cake polysaccharide |
| CN104906264A (en) * | 2015-07-01 | 2015-09-16 | 江南大学 | Novel alpha-glucosidase active inhibitor |
| CN105924484A (en) * | 2016-04-29 | 2016-09-07 | 中南林业科技大学 | Method for extracting quercetin derivatives from camellia oleifera leaves |
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