CN113181275A

CN113181275A - Fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and preparation method thereof

Info

Publication number: CN113181275A
Application number: CN202110527397.XA
Authority: CN
Inventors: 李大伟; 李华伟; 陈卓
Original assignee: Henan Yushang Biotechnology Group Co ltd
Current assignee: Henan Yushang Biotechnology Group Co ltd
Priority date: 2021-05-14
Filing date: 2021-05-14
Publication date: 2021-07-30

Abstract

The invention relates to a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and a preparation method thereof, wherein the fermented traditional Chinese medicine is prepared by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 34-55 parts of cimicifuga foetida, 55-65 parts of radix puerariae, 15-25 parts of schizonepeta, 15-25 parts of divaricate saposhnikovia root, 25-35 parts of fructus forsythiae, 20-30 parts of burdock, 25-35 parts of wild chrysanthemum, 20-30 parts of platycodon grandiflorum, 15-25 parts of almond, 15-25 parts of fructus aurantii, 15-25 parts of lithospermum, 5-15 parts of periostracum cicadae, 25-35 parts of bunge corydalis herb and 25-35 parts of dandelion; the various microorganisms include: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum. The traditional Chinese medicine active ingredients are mutually cooperated to activate the nonspecific immunity function of an organism, gastric mucosa injury is repaired by a plurality of beneficial bacteria, the upper layer cells of gastrointestinal mucosa exert the immunity function, and pathogens can not adhere to the intestinal wall, so that the bovine sarcoidosis is effectively prevented and treated.

Description

Fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicines for treating bovine sarcoidosis, and in particular relates to a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and a preparation method thereof.

Background

Bovine sarcoidosis, also known as dermatoma bovis, is caused by a DNA virus of the genus capripoxvirus of the family poxviridae. Endemic diseases are prevalent in sub-saharan south africa and in madagaska. Also in Egypt and Stokes. The disease is introduced into China in 2019, 8 and 12 months, which is the first diagnosis of the disease in China. The virus may be present in the saliva of infected cattle, and the disease may be infected by the contaminated environment. However, the most important mode of transmission is arthropod-mediated mechanical transmission, i.e., the biting of mosquitoes and flies, so that insect activity after rain frequently occurs in previously prevalent areas, and the disease is related to rainy seasons. With the mechanical transmission of viruses by mosquito and fly bites, viremia associated with leucocytes develops rapidly in the body.

The incidence of the nodular skin disease of the cattle is 5% -45%, the mortality rate is 1% -10%, although the mortality rate is not high, the utilization value of the nodular skin disease of the cattle is reduced because the diseased cattle is thinned and the milk yield is reduced, and the node is caved in a pot shape or a hole after the skin is tanned, so that the great economic loss can be caused. Except cow and cattle, buffalo, rabbit, sheep, goat, giraffe and the like can also be infected, and the incubation period of natural infection is 2-5 weeks. The sick cattle are the main infection source of the disease, and the virus exists in skin nodules, muscles, blood, viscera, saliva, nasal secretion and semen of the sick cattle and is often infected for more than 3 weeks after the sick cattle recover.

At present, no related vaccine for the disease exists in China, the treatment principle mainly aims at resisting virus, improving the immunity of the organism, diminishing inflammation and controlling infection, and environmental disinfection and biological safety measures are taken as assistance

Disclosure of Invention

The invention aims to provide a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and a preparation method thereof.

In order to achieve the purpose, the invention adopts the following technical scheme: a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 34-55 parts of cimicifuga foetida, 55-65 parts of radix puerariae, 15-25 parts of schizonepeta, 15-25 parts of divaricate saposhnikovia root, 25-35 parts of fructus forsythiae, 20-30 parts of burdock, 25-35 parts of wild chrysanthemum, 20-30 parts of platycodon grandiflorum, 15-25 parts of almond, 15-25 parts of fructus aurantii, 15-25 parts of lithospermum, 5-15 parts of periostracum cicadae, 25-35 parts of bunge corydalis herb and 25-35 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

Further preferably, the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 50 parts of cimicifuga foetida, 60 parts of radix puerariae, 20 parts of schizonepeta, 20 parts of radix sileris, 30 parts of fructus forsythiae, 25 parts of burdock, 30 parts of wild chrysanthemum, 25 parts of platycodon grandiflorum, 20 parts of almond, 20 parts of fructus aurantii, 20 parts of lithospermum, 10 parts of periostracum cicadae, 30 parts of bunge corydalis herb and 30 parts of dandelion.

A preparation method of a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis comprises the following steps:

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix Puerariae, herba Schizonepetae, radix Saposhnikoviae, fructus forsythiae, fructus Arctii, flos Chrysanthemi Indici, radix Platycodi, semen Armeniacae amarum, fructus Aurantii, radix Arnebiae, periostracum Cicadae, herba Violae, and herba Taraxaci, respectively pulverizing, and mixing with water to obtain Chinese medicinal mixture;

s2: respectively breeding aspergillus oryzae and aspergillus niger, and then inoculating the aspergillus oryzae and aspergillus niger into the traditional Chinese medicine mixture in S1 for primary aerobic fermentation to obtain primary fermented product;

s3: respectively breeding and culturing bacillus subtilis and bacillus licheniformis, and then inoculating the bacillus subtilis and the bacillus licheniformis into the first fermentation product obtained in the step S2 for secondary aerobic fermentation to obtain a second fermentation product;

s4: respectively breeding and culturing clostridium butyricum and lactobacillus plantarum, then inoculating the clostridium butyricum and lactobacillus plantarum into the second fermentation product obtained in S3 for anaerobic fermentation, and finally obtaining an anaerobic fermentation product;

s5, drying the anaerobic fermentation product in the step S4 at a low temperature to prepare dry Chinese medicinal powder, and then performing cold granulation at a low temperature to prepare the Chinese medicinal powder.

More preferably, the water adding amount of the water mixture in the S1 is 35-45% of the traditional Chinese medicine raw materials.

Further preferably, the breeding of the aspergillus oryzae and the aspergillus niger is to culture in a liquid culture medium, the culture temperature is 25-30 ℃, the rotating speed is 170-190 rpm, and the culture time is 15-20 hours; the breeding and culturing temperature of the bacillus subtilis and the bacillus licheniformis is 25-30 ℃, the rotating speed is 200-220 rpm, and the culturing time is 20-26 hours; the breeding and culturing temperature of the clostridium butyricum and the lactobacillus plantarum is 35-40 ℃, and the culturing time is 22-25 hours.

Further preferably, the temperature of the first aerobic fermentation is 25-30 ℃, the rotating speed is 80-100 rpm, and the fermentation time is 96-120 hours; the temperature of the second aerobic fermentation is 35-40 ℃, the rotating speed is 80-100 rpm, and the fermentation time is 48-72 hours; the temperature of anaerobic fermentation is 35-40 ℃, and the fermentation time is 22-26 hours.

s1, pulverizing cimicifugae rhizoma, radix Puerariae, herba Schizonepetae, radix Saposhnikoviae, fructus forsythiae, fructus Arctii, flos Chrysanthemi Indici, radix Platycodi, semen Armeniacae amarum, fructus Aurantii, radix Arnebiae, periostracum Cicadae, herba Violae, and herba Taraxaci, respectively, and extracting to obtain Chinese medicinal extractive solution;

s2: respectively breeding aspergillus oryzae and aspergillus niger, and then inoculating the aspergillus oryzae and aspergillus niger into the traditional Chinese medicine extract liquid in S1 for primary aerobic fermentation to obtain primary fermentation products;

s5: and concentrating the anaerobic fermentation product obtained in the step S4 to obtain the liquid traditional Chinese medicine.

Further preferably, the extraction time in S1 is 1-2 hours, and the extraction temperature is 75-85 ℃.

Has the advantages that: a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis and a preparation method thereof are obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, and the effective components of the traditional Chinese medicine are mutually cooperated to activate the nonspecific immunity function of an organism, phagocytize, inactivate and weaken pathogens, and simultaneously, various toxins in intestinal tracts are cleaned by a plurality of beneficial bacteria to repair the damage of gastric mucosa, so that the upper layer cells of the gastrointestinal tract mucosa can play the immunity function, and the pathogens can not adhere to the intestinal wall, thereby effectively preventing and treating the bovine sarcoidosis.

The recipe is mainly used for treating the symptoms of externally-expressed exanthema, eruption failure or unsmooth eruption, numbness of toxin, external pathogenic factors attacking the lung, pathogenic qi struggling with each other, disturbance of purification, wind-evil and exanthema toxin attacking the head and face, and heat scorching and body fluid injury. For treating exterior syndrome due to pungent and cool natured drugs, it can induce eruption and remove toxicity. In the formula, cimicifuga foetida, which is pungent, sweet and cold in nature, enters lung and stomach meridians, relieves muscles and promotes eruption, and clears heat and detoxifies as a monarch drug. The kudzu root is pungent, sweet and cool in taste, enters the stomach channel, relieves muscles and promotes eruption, and promotes the production of body fluid and removes heat, and is used as a ministerial drug. The two herbs are combined together to gently raise and disperse, and pass through the exterior and interior of the body surface, so they are the commonly used combination for treating eczema due to their actions of promoting the transmission of the pathogenic factors to the exterior of the body. In the formula, the peony root is used as an adjuvant drug for treating the diseases of red peony root, bitter in taste and cold in nature, entering blood system, clearing heat and cooling blood and promoting blood circulation. To harmonize the property of herbs with gan-Zhi-gan-Cao.

Drawings

FIG. 1 shows a process for preparing Chinese medicinal powder according to the present invention;

FIG. 2 shows a process for preparing Chinese medicinal liquid.

Detailed Description

The technical solution in the embodiment of the present invention will be clearly and completely described below with reference to fig. 1 to 2 in the embodiment of the present invention.

Example 1:

as shown in figure 1, a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 34 parts of cimicifuga foetida, 55 parts of radix puerariae, 15 parts of schizonepeta, 15 parts of divaricate saposhnikovia root, 25 parts of fructus forsythiae, 20 parts of great burdock achene, 25 parts of wild chrysanthemum, 20 parts of platycodon grandiflorum, 15 parts of almond, 15 parts of fructus aurantii, 15 parts of lithospermum, 5 parts of periostracum cicadae, 25 parts of bunge corydalis herb and 25 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

The fermented traditional Chinese medicine is prepared by the following raw materials according to the steps shown in figure 1:

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix Puerariae, herba Schizonepetae, radix Saposhnikoviae, fructus forsythiae, fructus Arctii, flos Chrysanthemi Indici, radix Platycodi, semen Armeniacae amarum, fructus Aurantii, radix Arnebiae, periostracum Cicadae, herba Violae, and herba Taraxaci, respectively pulverizing, and mixing with water to obtain Chinese medicinal mixture, wherein the water content is 35% of the Chinese medicinal materials;

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 35 ℃, the rotating speed is 170rpm, and the culture time is 15 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the fermented product into the traditional Chinese medicine mixture in S1 for primary aerobic fermentation to obtain a primary fermented product, wherein the temperature of the primary aerobic fermentation is 25 ℃, the rotating speed is 80rpm, and the fermentation time is 96 hours;

s3: respectively inoculating bacillus subtilis and bacillus licheniformis into a liquid culture medium for breeding culture, wherein the culture temperature is 25 ℃, the rotation speed is 200, and the culture time is 20 hours, so that the bacillus subtilis with antibacterial performance and high antibacterial peptide component of antibacterial active substances is bred, and the bacillus licheniformis with high active substance protease is produced by decomposing protein; then inoculating the fermented product into the first fermented product obtained in S2 for second aerobic fermentation to obtain a second fermented product, wherein the temperature of the second aerobic fermentation is 35 ℃, the rotating speed is 80rpm, and the fermentation time is 48 hours;

s4: the breeding technology belongs to the prior art and is not described herein any more. Inoculating the bred clostridium butyricum and lactobacillus plantarum into the second fermentation product obtained in S3 for anaerobic fermentation, and finally obtaining an anaerobic fermentation product, wherein the anaerobic fermentation temperature is 35 ℃, and the fermentation time is 22 hours;

Example 2:

as shown in figure 1, a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 34-55 parts of cimicifugae foetidae, 65 parts of kudzu vine root, 25 parts of schizonepeta, 25 parts of divaricate saposhnikovia root, 35 parts of weeping forsythia, 30 parts of great burdock achene, 35 parts of wild chrysanthemum, 30 parts of platycodon root, 25 parts of almond, 25 parts of bitter orange, 25 parts of lithospermum, 15 parts of cicada shell, 35 parts of bunge corydalis herb and 35 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 25 ℃, the rotating speed is 170rpm, and the culture time is 15 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and the Aspergillus oryzae strain which can utilize the polysaccharide substance in the polysaccharide component to purify the effective component of the traditional Chinese medicine, the breeding technique belongs to the prior art, and is not described again; then inoculating the fermented product into the traditional Chinese medicine mixture in S1 for primary aerobic fermentation to obtain a primary fermented product, wherein the temperature of the primary aerobic fermentation is 25 ℃, the rotating speed is 80rpm, and the fermentation time is 96 hours;

Example 3:

as shown in figure 1, a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 50 parts of cimicifuga foetida, 60 parts of radix puerariae, 20 parts of schizonepeta, 20 parts of radix sileris, 30 parts of fructus forsythiae, 25 parts of burdock, 30 parts of wild chrysanthemum, 25 parts of platycodon grandiflorum, 20 parts of almond, 20 parts of fructus aurantii, 20 parts of lithospermum, 10 parts of periostracum cicadae, 30 parts of bunge corydalis herb and 30 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix Puerariae, herba Schizonepetae, radix Saposhnikoviae, fructus forsythiae, fructus Arctii, flos Chrysanthemi Indici, radix Platycodi, semen Armeniacae amarum, fructus Aurantii, radix Arnebiae, periostracum Cicadae, herba Violae, and herba Taraxaci, respectively pulverizing, and mixing with water to obtain Chinese medicinal mixture, wherein the water content is 40% of the Chinese medicinal materials;

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 28 ℃, the rotating speed is 180rpm, and the culture time is 18 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the fermented product into the traditional Chinese medicine mixture in S1 for primary aerobic fermentation to obtain a primary fermented product, wherein the temperature of the primary aerobic fermentation is 28 ℃, the rotating speed is 90rpm, and the fermentation time is 108 hours;

s3: respectively inoculating bacillus subtilis and bacillus licheniformis into a liquid culture medium for breeding culture, wherein the culture temperature is 37 ℃, the rotation speed is 220, and the culture time is 24 hours, so that the bacillus subtilis with antibacterial performance and high antibacterial peptide component of antibacterial active substances is bred, and the bacillus licheniformis for producing high-activity substance protease is decomposed by protein; then inoculating the fermented product into the first fermented product obtained in S2 for second aerobic fermentation to obtain a second fermented product, wherein the temperature of the second aerobic fermentation is 37 ℃, the rotating speed is 90rpm, and the fermentation time is 60 hours;

s4: the breeding technology belongs to the prior art and is not described herein any more. Inoculating the bred clostridium butyricum and lactobacillus plantarum into the second fermentation product obtained in S3 for anaerobic fermentation, and finally obtaining an anaerobic fermentation product, wherein the anaerobic fermentation temperature is 37 ℃, and the fermentation time is 24 hours;

Example 4

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 30 ℃, the rotating speed is 170rpm, and the culture time is 15 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the fermented product into the traditional Chinese medicine mixture in S1 for primary aerobic fermentation to obtain a primary fermented product, wherein the temperature of the primary aerobic fermentation is 25 ℃, the rotating speed is 80rpm, and the fermentation time is 96 hours;

Example 5:

as shown in fig. 2, a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 34 parts of cimicifuga foetida, 55 parts of radix puerariae, 15 parts of schizonepeta, 15 parts of divaricate saposhnikovia root, 25 parts of fructus forsythiae, 20 parts of great burdock achene, 25 parts of wild chrysanthemum, 20 parts of platycodon grandiflorum, 15 parts of almond, 15 parts of fructus aurantii, 15 parts of lithospermum, 5 parts of periostracum cicadae, 25 parts of bunge corydalis herb and 25 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix puerariae, herba schizonepetae, radix sileris, fructus forsythiae, great burdock achene, wild chrysanthemum, platycodon grandiflorum, almond, fructus aurantii, lithospermum, periostracum cicadae, bunge corydalis herb and dandelion are respectively crushed and then extracted to obtain traditional Chinese medicine extract, wherein the extraction time is 1 hour, the extraction temperature is 75 ℃, and the extraction technology belongs to the prior art and is not repeated herein;

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 35 ℃, the rotating speed is 170rpm, and the culture time is 15 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the extract into the traditional Chinese medicine extract in S1 for primary aerobic fermentation to obtain a primary fermentation product, wherein the temperature of the primary aerobic fermentation is 25 ℃, the rotating speed is 80rpm, and the fermentation time is 96 hours;

s5, concentrating the anaerobic fermentation product obtained in the step S4 to obtain the liquid traditional Chinese medicine.

Example 6:

as shown in fig. 2, a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is obtained by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw materials in parts by weight: 50 parts of cimicifuga foetida, 60 parts of radix puerariae, 20 parts of schizonepeta, 20 parts of radix sileris, 30 parts of fructus forsythiae, 25 parts of burdock, 30 parts of wild chrysanthemum, 25 parts of platycodon grandiflorum, 20 parts of almond, 20 parts of fructus aurantii, 20 parts of lithospermum, 10 parts of periostracum cicadae, 30 parts of bunge corydalis herb and 30 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix puerariae, herba schizonepetae, radix sileris, fructus forsythiae, great burdock achene, wild chrysanthemum, platycodon grandiflorum, almond, fructus aurantii, lithospermum, periostracum cicadae, bunge corydalis herb and dandelion are respectively crushed and then extracted to obtain traditional Chinese medicine extract, wherein the extraction time is 1.5 hours, the extraction temperature is 80 ℃, and the extraction technology belongs to the prior art and is not repeated herein;

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 28 ℃, the rotating speed is 180rpm, and the culture time is 18 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the extract into the traditional Chinese medicine extract in S1 for primary aerobic fermentation to obtain a primary fermentation product, wherein the temperature of the primary aerobic fermentation is 28 ℃, the rotating speed is 90rpm, and the fermentation time is 108 hours;

Example 7:

s1, respectively taking the following traditional Chinese medicine raw materials in parts by weight: cimicifugae rhizoma, radix puerariae, herba schizonepetae, radix sileris, fructus forsythiae, great burdock achene, wild chrysanthemum, platycodon grandiflorum, almond, fructus aurantii, lithospermum, periostracum cicadae, bunge corydalis herb and dandelion are respectively crushed and then extracted to obtain traditional Chinese medicine extract, wherein the extraction time is 2 hours, the extraction temperature is 85 ℃, and the extraction technology belongs to the prior art and is not repeated herein;

s2: respectively inoculating aspergillus oryzae and aspergillus niger into a liquid culture medium for breeding culture, wherein the culture temperature is 30 ℃, the rotating speed is 190rpm, and the culture time is 20 hours, and inducing and breeding high-performance aspergillus niger strains capable of decomposing cellulose and lignin and breaking plant cell walls; and Aspergillus oryzae strain capable of purifying effective components of Chinese medicine by using polysaccharide substances in polysaccharide components, belonging to the prior art and not described herein again; then inoculating the extract into the traditional Chinese medicine extract in S1 for primary aerobic fermentation to obtain a primary fermentation product, wherein the temperature of the primary aerobic fermentation is 30 ℃, the rotating speed is 100rpm, and the fermentation time is 120 hours;

s3: respectively inoculating bacillus subtilis and bacillus licheniformis into a liquid culture medium for breeding culture, wherein the culture temperature is 30 ℃, the rotation speed is 220, and the culture time is 25 hours, so that the bacillus subtilis with antibacterial performance and high antibacterial peptide component of antibacterial active substances is bred, and the bacillus licheniformis with high active substance protease is produced by decomposing protein; then inoculating the fermented product into the first fermented product obtained in S2 for second aerobic fermentation to obtain a second fermented product, wherein the temperature of the second aerobic fermentation is 40 ℃, the rotating speed is 100rpm, and the fermentation time is 72 hours;

s4: the method comprises the steps of respectively inoculating clostridium butyricum and lactobacillus plantarum which are respectively bred and cultured into a culture medium for breeding and culturing, wherein the culture temperature is 40 ℃, the culture time is 25 hours, and lactobacillus with high lactic acid yield and clostridium butyricum with high butyric acid yield are bred. Inoculating the bred clostridium butyricum and lactobacillus plantarum into the second fermentation product obtained in S3 for anaerobic fermentation, and finally obtaining an anaerobic fermentation product, wherein the anaerobic fermentation temperature is 40 ℃, and the fermentation time is 26 hours;

The action mechanism is as follows:

1. various toxins in intestines are cleaned through a plurality of beneficial bacteria, gastrointestinal mucosa injury is repaired, cells on the upper layer of the gastrointestinal mucosa play an immune function, and pathogens cannot adhere to the intestinal wall. Lactic acid and butyric acid produced by the beneficial bacteria form a beneficial acid environment in the intestinal tract, can inactivate and weaken pathogens, and are discharged out of the body through the functions of clearing intestines and expelling toxin.

2. The medicine is stimulated by effective components of traditional Chinese medicines to activate the nonspecific immunity function of an organism, generate a large amount of macrophages, natural killer cells, neutrophils, lysozyme, defensins and immunoglobulin, and phagocytize, inactivate and weaken pathogens.

3. The effective components of the traditional Chinese medicine activate immune cells to generate interferon, transfer factors and the like, and block pathogen particles from adhering or penetrating into host cells, thereby achieving the effect of defending viruses.

4. The compound functions as follows: the Chinese medicine has polysaccharide, alkaloid and glycoside, which are perforated on the surface of virus protein to decompose protein and damage protein shell, and the virus nucleic acid structure is combined to prevent the duplication of its genetic gene and kill virus.

5. After the traditional Chinese medicine formula is fermented, the traditional Chinese medicine formula is anti-inflammatory, detumescent, muscle-expelling and eruption-promoting, and body fluid-producing and heat-removing, pathogens are weakened in toxicity and turned into weak toxicity under the action of nonspecific immunity, so that the functions of lymphocytes can be exerted, specific immune antibodies can be generated, and the rehabilitation effect can be achieved.

6. The metabolites of aspergillus niger and aspergillus oryzae can effectively adjust the rumen system of cattle, adjust the absorption capacity of animals to nutrition, improve the nonspecific immunity of animals, metabolize and decompose generated special amino acids, can effectively prevent DNA replication of DNA virus, and effectively prevent the replication of bovine nodavirus.

Clinical experiments are as follows:

experiment 1:

the solid traditional Chinese medicine powder is added into the feed according to the proportion of 2 percent, 35 cases are clinically cured after one week of administration, 40 cases are effective, the state after cure is good, the total effective rate reaches 75 percent, the prevention effect reaches 73 percent in the prevention process, and the feed has better effect on preventing and treating the bovine sarcoidosis.

Experiment 2:

the solid traditional Chinese medicine powder is added into the feed according to the proportion of 4 percent, 100 cattle with confirmed diagnosis are treated by administration, after one week of administration, 42 cattle with confirmed diagnosis are clinically cured, 45 cattle with confirmed diagnosis are effectively cured, the total effective rate reaches 87 percent, the expected effect is better, the prevention effect reaches 80 percent in the prevention process, and the cattle sarcoidosis can be effectively prevented and treated.

Experiment 3:

the liquid traditional Chinese medicine is added into drinking water according to the proportion of 2 per mill, 100 cattle with confirmed diagnosis are treated by administration, after one week of administration, 40 cattle are clinically cured, 38 cattle are effective, and the total effective rate reaches 78%. In the prevention process, the prevention effect reaches 68 percent, and the bovine sarcoidosis can be effectively prevented and treated.

Experiment 4:

the liquid traditional Chinese medicine is added into drinking water according to the proportion of 4 per mill, 100 cattle with confirmed diagnosis are treated by administration, 46 cattle with confirmed diagnosis are clinically cured after one week of administration, 42 cattle with confirmed diagnosis are effectively cured, the total effective rate reaches 88%, the expected effect is better, the prevention effect reaches 78% in the prevention process, the obvious effect on preventing and treating the bovine sarcoidosis is achieved, and the post-cure state is good.

The Chinese medicinal finished product has good prevention and treatment effects on bovine sarcoidosis inflammation and sarcoidosis virus; it can also be used for treating blain due to toxic heat, measles, fever, headache, cough, conjunctival congestion, epiphora, thirst, influenza, and acute tonsillitis; besides nodosis, it is also indicated for herpes zoster, herpes simplex, diarrhea, acute bacillary dysentery, and other diseases due to pathogenic factors stagnating in the muscles and lung and stomach heat.

The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by anyone in the light of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as those of the present application, fall within the protection scope of the present invention.

Claims

1. A fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is characterized in that: the microbial fertilizer is prepared by fermenting a plurality of microorganisms and traditional Chinese medicine raw materials, wherein the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 34-55 parts of cimicifuga foetida, 55-65 parts of radix puerariae, 15-25 parts of schizonepeta, 15-25 parts of divaricate saposhnikovia root, 25-35 parts of fructus forsythiae, 20-30 parts of burdock, 25-35 parts of wild chrysanthemum, 20-30 parts of platycodon grandiflorum, 15-25 parts of almond, 15-25 parts of fructus aurantii, 15-25 parts of lithospermum, 5-15 parts of periostracum cicadae, 25-35 parts of bunge corydalis herb and 25-35 parts of dandelion; the plurality of microorganisms includes: aspergillus oryzae, Aspergillus niger, Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum and Lactobacillus plantarum.

2. The fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 1, which is characterized in that: the traditional Chinese medicine raw materials comprise the following raw material medicines in parts by weight: 50 parts of cimicifuga foetida, 60 parts of radix puerariae, 20 parts of schizonepeta, 20 parts of radix sileris, 30 parts of fructus forsythiae, 25 parts of burdock, 30 parts of wild chrysanthemum, 25 parts of platycodon grandiflorum, 20 parts of almond, 20 parts of fructus aurantii, 20 parts of lithospermum, 10 parts of periostracum cicadae, 30 parts of bunge corydalis herb and 30 parts of dandelion.

3. A preparation method of a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is characterized by comprising the following steps: the method comprises the following steps:

4. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis, according to claim 3, is characterized in that: the water adding amount of the S1 mixture is 35-45% of the traditional Chinese medicine raw material.

5. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 3, characterized in that: the breeding of the aspergillus oryzae and the aspergillus niger is to culture in a liquid culture medium, wherein the culture temperature is 25-30 ℃, the rotating speed is 170-190 rpm, and the culture time is 15-20 hours; the breeding and culturing temperature of the bacillus subtilis and the bacillus licheniformis is 25-30 ℃, the rotating speed is 200-220 rpm, and the culturing time is 20-26 hours; the breeding and culturing temperature of the clostridium butyricum and the lactobacillus plantarum is 35-40 ℃, and the culturing time is 22-25 hours.

6. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 3, characterized in that: the temperature of the first aerobic fermentation is 25-30 ℃, the rotating speed is 80-100 rpm, and the fermentation time is 96-120 hours; the temperature of the second aerobic fermentation is 35-40 ℃, the rotating speed is 80 to

100rpm, and 48-72 hours of fermentation time; the temperature of anaerobic fermentation is 35-40 ℃, and the fermentation time is 22-26 hours.

7. A preparation method of a fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis is characterized by comprising the following steps: the method comprises the following steps:

8. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 7, characterized in that: the extraction time in S1 is 1-2 hours, and the extraction temperature is 75-85 ℃.

9. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 7, characterized in that: the breeding of the aspergillus oryzae and the aspergillus niger is to culture in a liquid culture medium, wherein the culture temperature is 25-30 ℃, the rotating speed is 170-190 rpm, and the culture time is 15-20 hours; the breeding and culturing temperature of the bacillus subtilis and the bacillus licheniformis is 25-30 ℃, the rotating speed is 200-220 rpm, and the culturing time is 20-26 hours; the breeding and culturing temperature of the clostridium butyricum and the lactobacillus plantarum is 35-40 ℃, and the culturing time is 22-25 hours.

10. The preparation method of the fermented traditional Chinese medicine for preventing and treating bovine sarcoidosis according to claim 7, characterized in that: the temperature of the first aerobic fermentation is 25-30 ℃, the rotating speed is 80-100 rpm, and the fermentation time is 96-120 hours; the temperature of the second aerobic fermentation is 35-40 ℃, the rotating speed is 80 to