CN114304429A - Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof - Google Patents
Traditional Chinese medicine composite probiotic feed additive for broiler chickens and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of livestock raising, in particular to a traditional Chinese medicine composite probiotic feed additive for broiler chickens and a preparation method thereof, aiming at the technical problems of low immunity, poor disease resistance and easy occurrence of diseases of the broiler chickens brought forward by the background technology, the following scheme is provided, wherein the traditional Chinese medicine composite probiotic feed additive comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, and the Chinese herbal medicine mixed powder comprises the following components in percentage: 5 to 10 percent of astragalus root, 3 to 5 percent of rhubarb, 5 to 10 percent of forsythia, 10 to 20 percent of isatis root, 1 to 5 percent of common andrographis herb, 10 to 15 percent of bupleurum, 1 to 5 percent of selfheal, 5 to 20 percent of wild chrysanthemum flower and 35 to 40 percent of heartleaf houttuynia herb. The feed additive adopts various traditional Chinese medicines, has good inhibiting effect on streptococcus, staphylococcus, escherichia coli, salmonella, shigella, chicken influenza virus and the like, enhances the immunity of broilers, and can improve intestinal probiotics of the broilers, reduce the diarrhea probability of the broilers and reduce the breeding loss by being mixed with lactobacillus, bifidobacterium and bacillus pumilus to be mixed into the feed.
Description
Technical Field
The invention relates to the technical field of livestock raising, in particular to a traditional Chinese medicine composite probiotic feed additive for broiler chickens and a preparation method thereof.
Background
In the process of livestock and poultry breeding, particularly in the process of breeding in a chicken farm, the amount of broiler motion is low, the immunity of chicken flocks is weak, particularly in autumn and winter, viral diseases are frequent, viral influenza is a common disease in each chicken farm, broiler diseases are easy to occur in the chicken farm, and if treatment measures are not timely taken, the death of chicks is easy to cause, the fat loss of adult chicks is reduced, the weight gain is reduced, the time of marketing is delayed, the disease resistance of the broiler is reduced, and huge economic loss is caused to farmers.
Pathogenic intestinal bacteria in broiler chickens can cause diarrhea, infection, abnormal liver function, and reduce digestion and absorption of nutrients. Beneficial bacteria inhibit the growth of various pathogenic bacteria through different mechanisms, stimulate the immune system, synthesize vitamins and the like. For many years, it has proven beneficial to use feed antibiotic additives which stabilize the intestinal flora, improve the growth performance of poultry and prevent certain specific intestinal pathologies, such as necrotic enteritis caused by clostridium perfringens. However, antibiotics eventually cause harmful bacteria in human bodies to become resistant to antibiotics after being used on animals, and currently, antibiotic growth promoters have been banned or limited in use in the breeding industry in many countries.
The traditional Chinese medicine is a treasure of Chinese culture, is inherited for thousands of years, treats both principal and secondary aspects of diseases, has high safety, no medicine residue and wide application, is used in combination with vaccines and western veterinary medicines, can effectively control and prevent the invasion of various bacteria and viruses to chickens, fully exerts the synergistic effect, promotes the improvement of the autoimmunity of the chickens, and thus plays an excellent role in preventing and treating diseases.
Disclosure of Invention
Based on the technical problems of low immunity, poor disease resistance and easy occurrence of diseases of the broiler chickens, the invention provides a traditional Chinese medicine composite probiotic feed additive for the broiler chickens and a preparation method thereof.
The invention provides a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises Chinese herbal medicine mixed powder and composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 5-10% of astragalus membranaceus, 3-5% of rheum officinale, 5-10% of fructus forsythiae, 10-20% of isatis root, 1-5% of andrographis paniculata, 10-15% of radix bupleuri, 1-5% of selfheal, 5-20% of wild chrysanthemum flower and 35-40% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 25-35% of peptone, 30-40% of agar, 10-25% of glucose, 3-5% of lactobacillus, 5-10% of bifidobacterium and 5-10% of bacillus pumilus.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
Preferably, the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
The invention also provides a preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into an aseptic environment, culturing for 48-72 h at 30-37 ℃, and respectively amplifying the three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, and atomizing and drying the strain into powder at low temperature to obtain strain powder;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing, boiling, centrifuging to extract Chinese herbal essence, and spray drying to obtain Chinese herbal essence powder;
s6: mixing the strain powder and the Chinese herbal medicine essence powder at a certain proportion, quantitatively packaging, and cold storing.
Preferably, the volume ratio of the bacterial liquid to the culture medium in the S3 is 3-5: 1, and the bacterial liquid and the crushed culture medium are shaken at the temperature of 30-37 ℃ at 200-300 r/min for 10-15 min.
Preferably, the residue and gauze filtered in S4 should be subjected to pressure filtration, and the pressure filtrate is collected and filtered, and the filtered pressure filtrate is combined with the filtrate, so as to avoid waste of bacteria liquid.
Preferably, in S5, the mixing time of the Chinese medicinal powder is 30min-60min, and the boiling time is 60min-120 min.
Preferably, the mixing mass ratio of the strain powder and the Chinese herbal medicine essence powder in the S6 is 1-1.5: 2.5-3.5.
The beneficial effects of the invention are as follows:
1. the effects of the Chinese herbal medicines are shown as follows;
the astragalus membranaceus has the effects of tonifying qi, strengthening the exterior, protecting the liver, promoting urination and enhancing the immune function of the chicken;
rhubarb: purgation, purging pathogenic fire, removing toxic substance, cooling blood, stopping bleeding, clearing away dampness and heat, and inhibiting viruses such as influenza, and has broad-spectrum antibacterial and antiviral effects, and can be used for preventing and treating streptococcus, staphylococcus, Diplococcus pneumoniae, Corynebacterium diphtheriae, Bacillus paratyphi, Bacillus dysenteriae, etc.;
fructus forsythiae: clearing away heat and toxic materials, relieving swelling, dispersing pathogen accumulation, dispelling pathogenic wind and heat, clearing heart fire, promoting urination, remarkably killing bacteria such as streptococcus, staphylococcus, pneumococcus, shigella, corynebacterium, escherichia coli, salmonella and proteus, and strongly inhibiting chicken influenza virus;
radix isatidis: clearing away heat and toxic materials, cooling blood, relieving sore throat, improving disease resistance of chicken, and inhibiting Bacillus, Streptococcus, Staphylococcus, Escherichia coli, Salmonella, Shigella, and chicken influenza virus;
andrographis paniculata: clearing heat and purging fire, promoting diuresis and relieving swelling, and has strong inhibiting effect on streptococcus, staphylococcus, salmonella, Escherichia coli, Pseudomonas aeruginosa, proteus, Diplococcus pneumoniae, etc.;
bupleurum root: relieving exterior syndrome, relieving fever, dispersing stagnated liver qi, relieving qi stagnation, regulating immunity, and resisting stress. Has strong inhibiting effect on chicken influenza virus;
selfheal: clearing heat and purging fire, resolving hard mass and detumescence, relieving cough and eliminating phlegm, relieving asthma and diminishing inflammation, and has strong antibacterial effect on staphylococcus, shigella, salmonella, pseudomonas, escherichia coli, tubercle bacillus, shigella dysenteriae, bacillus proteus, fungi and the like;
wild chrysanthemum flower: clearing heat and purging fire, removing toxic substances and relieving swelling, and has obvious inhibiting effect on staphylococcus, diphtheria bacillus, dysentery bacillus, chicken influenza virus, etc.;
houttuynia cordata: clearing away heat and toxic material, inducing diuresis, invigorating kidney, enhancing immunity, and resisting stress, and has inhibiting effect on Staphylococcus, Streptococcus, fungi and viruses.
In conclusion, the feed adopts various traditional Chinese medicines, has good inhibiting effect on streptococcus, staphylococcus, escherichia coli, salmonella, shigella, chicken influenza virus and the like, enhances the immunity of broiler chickens, and can improve intestinal probiotics of the broiler chickens, reduce the diarrhea probability of the broiler chickens and reduce the breeding loss by being mixed with lactobacillus, bifidobacterium and bacillus pumilus to be mixed into the feed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
The embodiment 1 discloses a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
The embodiment 2 discloses a traditional Chinese medicine composite probiotic feed additive for broiler chickens, which comprises a Chinese herbal medicine mixed powder and a composite probiotic powder, wherein the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
Embodiment 3, the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
The preparation method comprises the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium, wherein the volume ratio of the liquid to the culture medium is 3-5: 1, shaking the liquid and the crushed culture medium at the temperature of 30-37 ℃ for 10-15 min at 200-300 r/min to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into a sterile environment, culturing the three parts of bacterial suspension at the temperature of 30-37 ℃ for 48-72 h, and respectively amplifying three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, performing filter pressing on the filtered residue and gauze, collecting the filter pressing liquid for filtering, combining the filtered filter pressing liquid and the filtrate to avoid waste of the bacterial liquid, and performing low-temperature atomization drying on the mixture to obtain powder of the strain;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing the Chinese medicinal powder for 30-60 min, boiling for 60-120 min, centrifuging to extract Chinese medicinal essence, and spray drying to obtain Chinese medicinal essence powder;
s6: the strain powder and the Chinese herbal medicine essence powder are fully mixed according to the mixing mass ratio of the Chinese strain powder to the Chinese herbal medicine essence powder of 1-1.5: 2.5-3.5, and then are quantitatively packaged and stored in a cold storage mode.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A traditional Chinese medicine composite probiotic feed additive for broiler chickens comprises Chinese herbal medicine mixed powder and composite probiotic powder, and is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 5-10% of astragalus membranaceus, 3-5% of rheum officinale, 5-10% of fructus forsythiae, 10-20% of isatis root, 1-5% of andrographis paniculata, 10-15% of radix bupleuri, 1-5% of selfheal, 5-20% of wild chrysanthemum flower and 35-40% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 25-35% of peptone, 30-40% of agar, 10-25% of glucose, 3-5% of lactobacillus, 5-10% of bifidobacterium and 5-10% of bacillus pumilus.
2. The traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, which is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 10% of astragalus membranaceus, 5% of rheum officinale, 10% of fructus forsythiae, 10% of isatis root, 2% of andrographis paniculata, 10% of radix bupleuri, 3% of selfheal, 15% of wild chrysanthemum flower and 35% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: peptone 25%, agar 30%, glucose 25%, lactobacillus 3%, bifidobacterium 8% and bacillus pumilus 9%.
3. The traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, which is characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 5% of astragalus membranaceus, 3% of rheum officinale, 10% of fructus forsythiae, 20% of isatis root, 4% of andrographis paniculata, 10% of radix bupleuri, 1% of selfheal, 10% of wild chrysanthemum flower and 37% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 35% of peptone, 25% of agar, 20% of glucose, 4% of lactobacillus, 8% of bifidobacterium and 8% of bacillus pumilus.
4. The traditional Chinese medicine composite probiotic feed additive for broiler chickens and the preparation method thereof according to claim 1, characterized in that the Chinese herbal medicine mixed powder comprises the following components in percentage: 8% of astragalus membranaceus, 4% of rheum officinale, 8% of fructus forsythiae, 15% of isatis root, 5% of andrographis paniculata, 10% of radix bupleuri, 5% of selfheal, 20% of wild chrysanthemum flower and 25% of houttuynia cordata, wherein the composite probiotic powder comprises the following components in percentage by weight: 30% of peptone, 35% of agar, 15% of glucose, 5% of lactobacillus, 7% of bifidobacterium and 8% of bacillus pumilus.
5. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, characterized by comprising the following steps:
s1: respectively dissolving lactobacillus, bifidobacterium and bacillus pumilus samples collected from chicken intestinal tracts in sterilized sterile water, shaking at the temperature of between 30 and 37 ℃ at the speed of between 150 and 300r/min for 30 to 60min, dispersing flora, and respectively preparing bacterial liquids of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s2: taking peptone, glucose and agar according to mass percent, crushing the peptone and the agar, boiling, melting and mixing the peptone, the glucose and the agar, adjusting the pH value to 7.0 by using a sodium hydroxide solution, naturally cooling and sterilizing to obtain a culture medium;
s3: respectively taking liquid of lactobacillus, bifidobacterium and bacillus pumilus, dividing the prepared culture medium into three parts, crushing the culture medium, respectively placing the liquid of the lactobacillus, the liquid of the bifidobacterium and the liquid of the bacillus pumilus on the three parts of the culture medium to prepare three parts of bacterial suspension, placing the three parts of bacterial suspension into an aseptic environment, culturing for 48-72 h at 30-37 ℃, and respectively amplifying the three strains of the lactobacillus, the bifidobacterium and the bacillus pumilus;
s4: taking out the amplified strain, vibrating and crushing the strain by a centrifugal machine, and atomizing and drying the strain into powder at low temperature to obtain strain powder;
s5: pulverizing processed radix astragali, radix et rhizoma Rhei, fructus forsythiae, radix Isatidis, herba Andrographitis, bupleuri radix, Prunellae Spica, flos Chrysanthemi Indici and herba Houttuyniae to 100-200 mesh, mixing, boiling, centrifuging to extract Chinese herbal essence, and spray drying to obtain Chinese herbal essence powder;
s6: mixing the strain powder and the Chinese herbal medicine essence powder at a certain proportion, quantitatively packaging, and cold storing.
6. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens of claim 1, wherein the volume ratio of the bacterial liquid to the culture medium in S3 is 3-5: 1, the bacterial liquid and the crushed culture medium are shaken for 10min to 15min at the temperature of 30 ℃ to 37 ℃ at the speed of 200r/min to 300 r/min.
7. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein in S4, bacterial liquid needs to be filtered before atomization and drying, after 3-5 pieces of fine gauze are stacked, the bacterial suspension is filtered for 2-3 times until no residue is left on the fine gauze.
8. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein the residue filtered in S4 and gauze should be subjected to pressure filtration, and the pressure filtrate is collected and filtered, and the filtered pressure filtrate is combined with the filtrate to avoid waste of bacteria liquid.
9. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein in S5, the traditional Chinese medicine powder is mixed for 30min-60min, and the boiling time is 60min-120 min.
10. The preparation method of the traditional Chinese medicine composite probiotic feed additive for broiler chickens according to claim 1, wherein the mixing mass ratio of the strain powder and the Chinese herbal medicine essence powder in S6 is 1-1.5: 2.5-3.5.
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| CN114304428A (en) * | 2022-01-17 | 2022-04-12 | 清远一生自然生物研究院有限公司 | Preparation method of composite probiotic micro-ecological feed additive for meat ducks |
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