CN117298210B - Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry as well as preparation method and application thereof Download PDFInfo
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Classifications
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Mycology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a traditional Chinese medicine biological preparation for improving livestock and poultry immunity, a preparation method and application thereof, wherein the traditional Chinese medicine biological preparation is prepared from the following components in parts by mass: 1-3 parts of lactobacillus delbrueckii subsp bulgaricus, 1-3 parts of bifidobacterium longum, 1-3 parts of lactobacillus amylovorus, 1-2 parts of phytase, 1-2 parts of xylanase, 1-2 parts of beta-glucanase, 5-10 parts of astragalus membranaceus, 10-15 parts of radix bupleuri, 1-5 parts of Chinese yam, 15-20 parts of dyers woad leaf, 8-15 parts of honeysuckle stem, 8-12 parts of centella asiatica, 10-15 parts of prune, 5-10 parts of hibiscus and 5-10 parts of ampelopsis japonica. The traditional Chinese medicine preparation fermented by probiotics adopts a mode of synergistic fermentation of bacterial enzymes, can obviously improve the immunity of livestock, strengthen the immunity of livestock organisms and effectively prevent and treat immunosuppressive diseases.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a traditional Chinese medicine biological preparation for improving livestock immunity, and a preparation method and application thereof.
Background
With the expansion of the breeding scale of animal husbandry, the breeding density of domestic animals such as pigs, cattle, sheep and the like is gradually expanded, the activity space of the domestic animals is reduced, and once the domestic animals are infected with viral diseases, the domestic animals are easy to be infected on a large scale and even die, so that the breeding benefit of the domestic animals is seriously damaged. Common viral diseases in livestock breeding are foot-and-mouth disease, pseudorabies, porcine reproductive and respiratory syndrome, swine fever, sheep pox, sheep fever and the like.
Because the pathogenesis of the viral diseases of livestock and poultry is complex, large-scale outbreaks easily occur after the viral diseases are infected in the breeding process, the problems of drug resistance enhancement of viruses caused by excessive dosage of antibiotics, repeated immunization of vaccines and the like cannot be achieved, the better curative effect cannot be obtained, the prevention and treatment difficulty is increased, the breeding cost is additionally increased, and the diseased animals finally end up dying.
The traditional Chinese medicine has excellent control effect on livestock and poultry viral diseases, can keep the safety of livestock and poultry products, and has wide application prospect.
Patent CN112425684B discloses a fermented feed additive with immunity enhancing effect and its preparation method, adding cellulase and neutral protease into astragalus root, honeysuckle flower, chicken's gizzard-skin and wheat bran for enzymolysis, then adding bacillus subtilis and hansenula polymorpha for fermentation. Although the traditional Chinese medicine is subjected to the synergistic fermentation of bacterial enzymes, the immune effect of the traditional Chinese medicine is still to be improved.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide a traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry, and a preparation method and application thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme:
The invention provides a traditional Chinese medicine biological preparation for improving livestock and poultry immunity, which is prepared from the following components in parts by mass:
1-3 parts of lactobacillus delbrueckii subsp bulgaricus, 1-3 parts of bifidobacterium longum, 1-3 parts of lactobacillus amylovorus, 1-2 parts of phytase, 1-2 parts of xylanase, 1-2 parts of beta-glucanase, 5-10 parts of astragalus membranaceus, 10-15 parts of radix bupleuri, 1-5 parts of Chinese yam, 15-20 parts of dyers woad leaf, 8-15 parts of honeysuckle stem, 8-12 parts of centella asiatica, 10-15 parts of prune, 5-10 parts of hibiscus and 5-10 parts of ampelopsis japonica.
Preferably, the lactobacillus delbrueckii subspecies bulgaricus has a deposit number of CICC 20247, the bifidobacterium longum has a deposit number of CICC 6192, and the lactobacillus amylovorus has a deposit number of CICC 6090.
Preferably, the total viable count of the Lactobacillus delbrueckii subspecies bulgaricus, bifidobacterium longum and Lactobacillus amylovorus is more than or equal to 10 9 CFU/g.
Preferably, the enzyme activities of the phytase, xylanase and beta-glucanase are all more than or equal to 10000u/g.
The second aspect of the invention provides a preparation method of the traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry, which comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis, adding into water, and sterilizing to obtain Chinese medicinal mixture; adding phytase, xylanase and beta-glucanase into the traditional Chinese medicine mixture for enzymolysis, and inactivating enzyme to obtain an enzymolysis product;
(2) Adding Lactobacillus delbrueckii subsp bulgaricus, bifidobacterium longum and Lactobacillus amylovorus into the enzymolysis product, performing anaerobic fermentation, and lyophilizing to obtain the traditional Chinese medicine biological preparation.
Preferably, in the step (1), the crushed granularity is 300 meshes, and the adding amount of water is 10 times of the total weight of the traditional Chinese medicine; the enzymolysis temperature is 35-40 ℃ and the enzymolysis time is 3-5h.
Preferably, in the step (2), the anaerobic fermentation temperature is 35-37 ℃ and the anaerobic fermentation time is 40-50h.
The third aspect of the invention provides an application of the traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry in improving the immunity of livestock and poultry.
Preferably, the traditional Chinese medicine preparation is mixed in the feed, the addition amount of the traditional Chinese medicine preparation is 1-5% of the weight of the feed, and the feed is fed for 2 times a day.
The traditional Chinese medicine biological preparation consists of traditional Chinese medicines, a composite microbial inoculum and a composite enzyme agent. The nutrients contained in the traditional Chinese medicine components provide a growth basis for the propagation of the compound microbial inoculum, and the fermentation of the compound microbial inoculum and the compound enzyme preparation can change the effective components in the traditional Chinese medicine, so that the effect of the original traditional Chinese medicine is improved after fermentation, and the action function of the traditional Chinese medicine is expanded.
The Chinese medicinal components comprise astragalus root, bupleurum root, chinese yam, dyers woad leaf, honeysuckle stem, centella asiatica, geum japonicum, hibiscus and Japanese ampelopsis root, and have the effects of clearing heat, detoxicating, tonifying qi and strengthening exterior and improving the immunity of livestock.
Various nutrients such as amino acids, vitamins, digestive enzymes and the like are also produced in the growth and propagation process of probiotics, so that nutrients are provided for livestock, the digestion and absorption functions are improved, and the feed utilization rate is improved.
According to the invention, the traditional Chinese medicine is subjected to enzymolysis and then fermentation, so that cell walls of most plants in the traditional Chinese medicine can be crushed to release active ingredients rapidly, and the substrate after enzymolysis can be better reused by the composite microbial inoculum during fermentation. The synergistic fermentation of the bacterial enzymes plays a key role in the process of fermenting, degrading and converting the Chinese herbal medicine into new components. The compound microbial inoculum and the compound enzyme preparation have synergistic promotion effect on improving the immunity of organisms.
The invention has the beneficial effects that:
The invention gives play to the characteristic advantages of the compatibility of traditional Chinese medicines, and uses astragalus, bupleurum, chinese yam, dyers woad leaf, honeysuckle stem, centella asiatica, geum japonicum, hibiscus and ampelopsis japonica to achieve the effect of 'keeping healthy qi in the body, and avoiding pathogenic factors from drying', thus realizing the regulation of yin-yang balance. The traditional Chinese medicine preparation fermented by probiotics adopts a mode of synergistic fermentation of bacterial enzymes, can obviously improve the immunity of livestock, strengthen the immunity of the livestock, effectively prevent and treat immunosuppressive diseases, and solve the problems of bacterial drug resistance, drug residues, toxic and side effects and the like caused by antibiotics.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As described in the background art, the problems of overdose of antibiotics and repeated immunization of vaccines, etc. result in enhanced drug resistance of viruses, and better curative effect cannot be obtained, but the difficulty of prevention and treatment is increased.
Based on the knowledge and treatment principle of traditional Chinese medicine on livestock viral diseases, the invention follows the research of traditional Chinese medicine compatibility principle and modern pharmacology on traditional Chinese medicine functions, screens a plurality of traditional Chinese veterinary medicine related books such as the pharmacopoeia of the people's republic of China (2020 edition) according to the traditional Chinese veterinary medicine theory, combines a large number of experimental verification, selects dyers woad leaf and bupleurum as monarch drugs, centella asiatica, honeysuckle stem and shrubalthea as ministerial drugs, uses geum japonicum and Japanese ampelopsis as adjuvant drugs, uses astragalus mongholicus and Chinese yam as conductant drugs, and plays the roles of clearing heat, detoxicating, tonifying qi and consolidating exterior through reasonable compatibility of monarch and ministerial drugs, and improves the immunity of livestock.
In view of the fact that the traditional Chinese medicine decoction has high destructiveness on effective components, low availability of the effective components and poor palatability, and microorganisms are used for fermenting the traditional Chinese medicine, the effective components of the traditional Chinese medicine can be protected from destruction to the greatest extent, therefore, 8 strains with fermenting capability on the traditional Chinese medicine are screened out of purchased strains by taking alkaloid and polysaccharide content of the traditional Chinese medicine as indexes, then interaction relations among the 8 strains are examined by adopting a counter experiment, and the counter experiment result shows that: the Lactobacillus delbrueckii subsp bulgaricus, bifidobacterium longum and Lactobacillus amylovorus have no antagonistic bands, and can be used in combination. Based on the above results, it was found that the combination of three strains of Lactobacillus delbrueckii subspecies bulgaricus, bifidobacterium longum and Lactobacillus amylovorus, which were selected from two or three of Lactobacillus delbrueckii subspecies bulgaricus, bifidobacterium longum and Lactobacillus amylovorus, was the best fermentation effect on the traditional Chinese medicine.
In order to enable those skilled in the art to more clearly understand the technical scheme of the present application, the technical scheme of the present application will be described in detail with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and are commercially available. The Lactobacillus delbrueckii subsp bulgaricus (Lactobacillus delbrueckii subsp. Bulgaricus) used in the following examples and comparative examples of the present invention was purchased from the China center for Industrial microorganism culture Collection, accession number CICC 20247, the bifidobacterium longum (Bifidobacterium longum) was deposited with accession number CICC 6192, and the Lactobacillus amylovorus (Lactobacillus amylovorus) was deposited with number CICC 6090; phytase, xylanase, beta-glucanase were purchased from shandong duckweed biotechnology limited; astragalus root, bupleurum root, chinese yam, dyers woad leaf, honeysuckle stem, centella asiatica, geum japonicum, hibiscus, ampelopsis japonica are purchased from Teng Wang pharmaceutical industry company in Bozhou.
Example 1
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: lactobacillus delbrueckii subsp bulgaricus 1g, bifidobacterium longum 1g, lactobacillus amylovorus 1g, phytase 1g, xylanase 1g, beta-glucanase 1g, astragalus root 5g, bupleurum root 10g, yam 1g, dyers woad leaf 15g, honeysuckle stem 8g, centella asiatica 8g, prune 10g, hibiscus 5g and ampelopsis 5g. The total viable count is more than or equal to 10 9 CFU/g.
2. The preparation method comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis into 300 mesh, and adding 670g water to obtain Chinese medicinal mixture;
(2) Adding Lactobacillus delbrueckii subsp Bulgaria, bifidobacterium longum, lactobacillus amylovorus, phytase, xylanase and beta-glucanase into the Chinese medicinal mixture, anaerobic fermenting at 35-37deg.C for 48 hr, freezing, and drying to obtain Chinese medicinal biological preparation.
Example 2
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: lactobacillus delbrueckii subsp bulgaricus 1g, bifidobacterium longum 2g, lactobacillus amylovorus 3g, phytase 1g, xylanase 2g, beta-glucanase 2g, astragalus 8g, radix bupleuri 12g, yam 3g, dyers woad leaf 18g, honeysuckle 10g, centella 10g, prune 12g, hibiscus 8g and ampelopsis 8g. The total viable count is more than or equal to 10 9 CFU/g.
2. The preparation method comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, radix Ampelopsis into 300 mesh, adding into 890g water, and sterilizing to obtain Chinese medicinal mixture; adding phytase, xylanase and beta-glucanase into the traditional Chinese medicine mixture, carrying out enzymolysis for 4 hours at 37 ℃, and inactivating enzyme to obtain an enzymolysis product;
(2) Adding Lactobacillus delbrueckii subsp bulgaricus, bifidobacterium longum, lactobacillus amylovorus, phytase, xylanase and beta-glucanase into the enzymolysis product, performing anaerobic fermentation at 35-37deg.C for 48 hr, freezing, and drying to obtain the final product.
Example 3
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: lactobacillus delbrueckii subsp bulgaricus 3g, bifidobacterium longum 2g, lactobacillus amylovorus 1g, phytase 2g, xylanase 1g, beta-glucanase 1g, astragalus root 10g, bupleurum root 15g, yam 5g, dyers woad leaf 20g, honeysuckle stem 15g, centella asiatica 12g, prune 15g, hibiscus 10g and ampelopsis 10g. The total viable count is more than or equal to 10 9 CFU/g.
2. The preparation method comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, radix Ampelopsis into 300 mesh, adding 1120g water, and sterilizing to obtain Chinese medicinal mixture; adding phytase, xylanase and beta-glucanase into the traditional Chinese medicine mixture, carrying out enzymolysis for 4 hours at 37 ℃, and inactivating enzyme to obtain an enzymolysis product;
(2) Adding Lactobacillus delbrueckii subsp bulgaricus, bifidobacterium longum, lactobacillus amylovorus, phytase, xylanase and beta-glucanase into the enzymolysis product, performing anaerobic fermentation at 35-37deg.C for 48 hr, freezing, and drying to obtain the final product.
Comparative example 1
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: lactobacillus delbrueckii subspecies bulgaricus 3g, bifidobacterium longum 2g, lactobacillus amylovorus 1g, astragalus mongholicus 10g, radix bupleuri 15g, yam 5g, dyers woad leaf 20g, honeysuckle 15g, centella asiatica 12g, prune 15g, hibiscus 10g and ampelopsis 10g. The total viable count is more than or equal to 10 9 CFU/g.
2. The preparation method comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis into 300 mesh, adding 1120g water to obtain Chinese medicinal mixture;
(2) Adding Lactobacillus delbrueckii subsp Bulgaria, bifidobacterium longum, lactobacillus amylovorus, phytase, xylanase and beta-glucanase into the Chinese medicinal mixture, anaerobic fermenting at 35-37deg.C for 48 hr, freezing, and drying to obtain Chinese medicinal biological preparation.
In this comparative example, fermentation was performed using only the complex microbial agent as compared with example 3, and the other steps were the same as in example 3.
Comparative example 2
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: 2g of phytase, 1g of xylanase, 1g of beta-glucanase, 10g of astragalus, 15g of bupleurum, 5g of Chinese yam, 20g of dyers woad leaf, 15g of honeysuckle stem, 12g of centella asiatica, 15g of salicyl plum, 10g of hibiscus and 10g of ampelopsis japonica.
2. The preparation method comprises the following steps:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis into 300 mesh, adding 1120g water, and sterilizing to obtain Chinese medicinal mixture; adding phytase, xylanase and beta-glucanase into the traditional Chinese medicine mixture, carrying out enzymolysis for 4 hours at 37 ℃, and inactivating enzyme to obtain an enzymolysis product;
(2) Placing the enzymolysis product at 35-37deg.C for 48 hr, and lyophilizing to obtain Chinese medicinal preparation.
In this comparative example, fermentation was performed using only the complex enzyme preparation as compared with example 3, and the rest was the same as in example 3.
Comparative example 3
Traditional Chinese medicine biological preparation for improving immunity of livestock and poultry
1. The raw material components are as follows: 10g of astragalus, 15g of radix bupleuri, 5g of Chinese yam, 20g of dyers woad leaf, 15g of honeysuckle stem, 12g of centella asiatica, 15g of prune, 10g of hibiscus and 10g of ampelopsis japonica.
2. The preparation method comprises the following steps:
Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis into 300 mesh, adding 1120g water to obtain a Chinese medicinal mixture, and drying to obtain Chinese medicinal biological preparation.
In this comparative example, the fermentation was performed without using the complex microbial agent and the complex enzyme preparation, compared with example 3, and the rest was the same as in example 3.
Experimental example 1
The method is carried out according to the step in the literature 'optimized extraction of Chinese herbal compound polysaccharide and the influence of the Chinese herbal compound polysaccharide on the immune function of mice' (Zhang Zian. Anhui agricultural university, 2014).
Selecting 140 mice of 18-20g of each male and female half, randomly dividing the mice into six treatment groups and a blank control group, and feeding the normal daily ration of the mice into the first treatment group and the traditional Chinese medicine biological preparation prepared in the example 1; the second treatment group was fed normal daily ration of mice + the traditional Chinese medicine biological preparation prepared in example 2; the third treatment group was fed normal daily ration of mice + the traditional Chinese medicine preparation prepared in example 3; the fourth treatment group was fed normal daily ration of mice + the traditional Chinese medicine biological preparation prepared in comparative example 1; the fifth treatment group fed normal daily ration of mice + the traditional Chinese medicine biological preparation prepared in comparative example 2; the sixth treatment group fed normal daily ration of mice + the traditional Chinese medicine biological preparation prepared in comparative example 3; the blank control group was fed normal daily diet only to mice. The normal daily ration of mice fed by each group is the same, and the amount of the traditional Chinese medicine biological preparation is 5% of the normal daily ration of the mice. Feeding was continued for 7 days.
The mice are numbered and weighed in each treatment group, then the mice are sacrificed after neck breakage, the thymus and spleen of the immune organs are rapidly taken, blood stains are removed by sucking with filter paper, surrounding tissues, fat and the like are removed, and the wet weight is called. The immune organ index was calculated and the experimental results are shown in table 1. The formula is as follows:
Spleen index = spleen weight (mg)/mouse body weight (g) x 100%.
Thymus index = thymus weight (mg)/mice weight (g) x 100%.
TABLE 1 immune organ index of mice in each treatment group
。
The traditional Chinese medicine biological preparation prepared by the invention can obviously improve the spleen index and thymus index of mice, can improve the immunity of the mice and can enhance the immunity performance of the mice.
Experimental example 2
The procedure in literature "study of the effect of compound traditional Chinese medicine on growth performance and immune function of beef calves" (Wang Ran. University of southwest, 2016.) was followed, as follows.
The experimental date is 2023, 3, 1, 5, 2, 7, and the formal experiment is carried out for 8 cycles, and the feeding cycle of each treatment group is 7 days, wherein the feeding is 5 days, and the stopping is 2 days. The basal diet is fed for 2 times per day, the feeding time is 8 am and 5 pm, and 3.5 kg/head per day. The house was cleaned daily and disinfected periodically.
Selecting 70 healthy and disease-free weaned calves about 4 months old, randomly dividing the weaned calves into six treatment groups and a blank control group, and adding the traditional Chinese medicine biological preparation prepared in the example 1 into basic ration of the first treatment group; adding the traditional Chinese medicine biological preparation prepared in the example 2 into the basic ration of the second treatment group; adding the traditional Chinese medicine biological preparation prepared in the example 3 into the basic ration of the third treatment group; adding the traditional Chinese medicine biological preparation prepared in the comparative example 1 into the basic ration of the fourth treatment group; adding the traditional Chinese medicine biological preparation prepared in the comparative example 2 into the basic ration of the fifth treatment group; adding the traditional Chinese medicine biological preparation prepared in the comparative example 3 into the basic ration of the sixth treatment group; the blank control group was fed only basal diet. The addition of the traditional Chinese medicine biological preparation in each treatment group is the same and is 3% of the mass of the basic ration. The amount of basal diet fed to each treatment group was the same, and the basal diet composition is shown in table 2.
TABLE 2 composition and content of basic ration
。
The premix is a compound premix feed for the beef cattle of the Ying Er Mei No. 1 series. The components are mass fractions.
And after the experiment is finished, calf venous blood is collected, a serum sample detection enzyme-linked immunosorbent assay is carried out, the enzyme-linked immunosorbent assay is carried out according to the steps of the instruction book of the kit, and the experimental result is shown in table 3.
TABLE 3 serum immune index and morbidity for each treatment and blank
。
The traditional Chinese medicine biological preparation prepared by the invention obviously improves the immunity of organisms, and the IgA, igG, igM content in serum is greatly improved compared with a blank control group, so that the immunity of organisms is greatly improved. The composite microbial inoculum and the composite enzyme preparation used by the invention have synergistic promotion effect in improving the immunity of organisms.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (6)
1. The traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry is characterized by being prepared from the following components in parts by mass:
1-3 parts of lactobacillus delbrueckii subsp bulgaricus, 1-3 parts of bifidobacterium longum, 1-3 parts of lactobacillus amylovorus, 1-2 parts of phytase, 1-2 parts of xylanase, 1-2 parts of beta-glucanase, 5-10 parts of astragalus membranaceus, 10-15 parts of radix bupleuri, 1-5 parts of Chinese yam, 15-20 parts of dyers woad leaf, 8-15 parts of honeysuckle stem, 8-12 parts of centella asiatica, 10-15 parts of prune, 5-10 parts of hibiscus and 5-10 parts of ampelopsis japonica;
the traditional Chinese medicine biological preparation is prepared by the following method:
(1) Pulverizing radix astragali, bupleuri radix, rhizoma Dioscoreae, folium Isatidis, caulis Lonicerae, herba Centellae, fructus Myricae Rubrae, hibisci, and radix Ampelopsis, adding into water, and sterilizing to obtain Chinese medicinal mixture; adding phytase, xylanase and beta-glucanase into the traditional Chinese medicine mixture for enzymolysis, and inactivating enzyme to obtain an enzymolysis product;
(2) Adding Lactobacillus delbrueckii subsp bulgaricus, bifidobacterium longum and Lactobacillus amylovorus into the enzymolysis product, performing anaerobic fermentation, and freeze-drying to obtain a traditional Chinese medicine biological preparation;
The Lactobacillus delbrueckii subspecies bulgaricus has a preservation number of CICC 20247, the bifidobacterium longum has a preservation number of CICC 6192, and the Lactobacillus amylovorus has a preservation number of CICC 6090.
2. The traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry according to claim 1, wherein the total viable count of lactobacillus delbrueckii subspecies bulgaricus, bifidobacterium longum and lactobacillus amylovorus is more than or equal to 10 9 CFU/g.
3. The traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry according to claim 1, wherein the enzyme activities of phytase, xylanase and beta-glucanase are all more than or equal to 10000u/g.
4. The method for preparing a biological Chinese medicine preparation for improving immunity of livestock and poultry according to claim 1, wherein in the step (1), the crushed granularity is 300 meshes, and the adding amount of water is 10 times of the total weight of the Chinese medicine; the enzymolysis temperature is 35-40 ℃ and the enzymolysis time is 3-5h.
5. The method for preparing the traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry according to claim 1, wherein in the step (2), the anaerobic fermentation temperature is 35-37 ℃, and the anaerobic fermentation time is 40-50h.
6. The use of the traditional Chinese medicine biological preparation for improving livestock and poultry immunity according to any one of claims 1-5 in preparation of the preparation for improving livestock and poultry immunity.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1935182A (en) * | 2005-09-20 | 2007-03-28 | 张伟巍 | Chinese herbal medicine compound immunopotentiator for animal |
| CN106036040A (en) * | 2016-07-31 | 2016-10-26 | 镇江天和生物技术有限公司 | Mixed feed additive |
| CN111011643A (en) * | 2019-12-31 | 2020-04-17 | 山西大禹生物工程股份有限公司 | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935182A (en) * | 2005-09-20 | 2007-03-28 | 张伟巍 | Chinese herbal medicine compound immunopotentiator for animal |
| CN106036040A (en) * | 2016-07-31 | 2016-10-26 | 镇江天和生物技术有限公司 | Mixed feed additive |
| CN111011643A (en) * | 2019-12-31 | 2020-04-17 | 山西大禹生物工程股份有限公司 | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof |
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