CN114846134B - 一种间充质干细胞 - Google Patents

一种间充质干细胞 Download PDF

Info

Publication number
CN114846134B
CN114846134B CN202080078206.XA CN202080078206A CN114846134B CN 114846134 B CN114846134 B CN 114846134B CN 202080078206 A CN202080078206 A CN 202080078206A CN 114846134 B CN114846134 B CN 114846134B
Authority
CN
China
Prior art keywords
cell
less
lngfr
mesenchymal stem
coefficient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080078206.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN114846134A (zh
Inventor
松崎有未
陶山隆史
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prek Co ltd
Original Assignee
Prek Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prek Co ltd filed Critical Prek Co ltd
Publication of CN114846134A publication Critical patent/CN114846134A/zh
Application granted granted Critical
Publication of CN114846134B publication Critical patent/CN114846134B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/10—Production naturally occurring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080078206.XA 2020-01-16 2020-04-06 一种间充质干细胞 Active CN114846134B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP2020/002197 WO2021144995A1 (ja) 2020-01-16 2020-01-16 高純度間葉系幹細胞
JPPCT/JP2020/002197 2020-01-16
PCT/JP2020/016228 WO2021145002A1 (ja) 2020-01-16 2020-04-06 高純度間葉系幹細胞

Publications (2)

Publication Number Publication Date
CN114846134A CN114846134A (zh) 2022-08-02
CN114846134B true CN114846134B (zh) 2024-10-29

Family

ID=76864087

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080078206.XA Active CN114846134B (zh) 2020-01-16 2020-04-06 一种间充质干细胞

Country Status (9)

Country Link
US (1) US20230002735A1 (https=)
EP (1) EP4053266A4 (https=)
JP (2) JP7466218B2 (https=)
KR (1) KR20220084078A (https=)
CN (1) CN114846134B (https=)
AU (1) AU2020423706B2 (https=)
CA (1) CA3163517C (https=)
IL (1) IL293724A (https=)
WO (2) WO2021144995A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022177032A1 (ja) * 2021-02-19 2022-08-25 ＰｕＲＥＣ株式会社 骨疾患治療用医薬組成物
AU2024354962A1 (en) 2023-10-02 2026-03-12 National University Corporation Tokai National Higher Education And Research System Method for managing quality of stem cells by image analysis using ai
TW202529782A (zh) * 2023-10-05 2025-08-01 日商ＰｕＲＥＣ股份有限公司 含有高純度間葉系幹細胞之用以治療以及／或是預防粒線體疾病之組成物

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6025329B2 (ja) 1980-07-25 1985-06-18 株式会社日立製作所 鋼管移送騒音防止装置
DE102006043625B4 (de) * 2006-09-12 2012-02-23 Eberhard-Karls-Universität Tübingen Universitätsklinikum Verfahren zur Isolierung und/oder Identifizierung von mesenchymalen Stammzellen (MSC)
JP2009060840A (ja) * 2007-09-06 2009-03-26 Keio Gijuku ヒト間葉系幹細胞濃縮方法
EP3124601A1 (en) * 2008-08-14 2017-02-01 Mesoblast International Sàrl Purified mesenchymal stem cell compositions and methods of purifying mesenchymal stem cell compositions
JP6463029B2 (ja) 2013-08-02 2019-01-30 有未 伊谷 ヒト間葉系幹細胞を特異的に認識するモノクローナル抗体並びにこれを用いたヒト間葉系幹細胞の分離及び／または品質評価を行う方法
WO2016017795A1 (ja) * 2014-08-01 2016-02-04 有未 伊谷 ヒト間葉系幹細胞の品質評価方法、及び、そのためのモノクローナル抗体
KR20160017795A (ko) 2014-08-05 2016-02-17 삼성디스플레이 주식회사 박막 트랜지스터 기판, 이의 제조 방법, 및 박막 트랜지스터 기판을 포함하는 표시 장치
EP3336177A1 (fr) * 2016-12-16 2018-06-20 Stem Cell Vet Therapeutics Procede de culture, d'isolement et d'enrichissement de cellules souches mesenchymateuses clonogeniques, a fort rendement, en vue d'une utilisation therapeutique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"LNGFR+THY-1+VCAM-1hi+ Cells Reveal Functionally Distinct Subpopulations in Mesenchymal Stem Cells";Yo Mabuchi et al.;《Stem Cell Reports》;20130806;第1卷;第152-165页和补充实验材料第1-14页 *

Also Published As

Publication number Publication date
CN114846134A (zh) 2022-08-02
WO2021145002A8 (ja) 2022-06-30
WO2021145002A1 (ja) 2021-07-22
JPWO2021145002A1 (https=) 2021-07-22
JP7466218B2 (ja) 2024-04-12
CA3163517A1 (en) 2021-07-22
AU2020423706A1 (en) 2022-06-23
IL293724A (en) 2022-08-01
US20230002735A1 (en) 2023-01-05
JP2024089675A (ja) 2024-07-03
WO2021144995A1 (ja) 2021-07-22
EP4053266A1 (en) 2022-09-07
CA3163517C (en) 2025-05-13
EP4053266A4 (en) 2023-01-11
JP7792151B2 (ja) 2025-12-25
KR20220084078A (ko) 2022-06-21
AU2020423706B2 (en) 2024-10-24

Similar Documents

Publication Publication Date Title
JP7792151B2 (ja) 高純度間葉系幹細胞
JP3160600B2 (ja) ヒト造血幹細胞
JP6038962B2 (ja) Ｚｎｆ２８１を発現する臍帯血由来の万能幹細胞の分離方法
US8163495B2 (en) Method for isolating and/or identifying mesenchymal stem cells (MSC)
KR102490731B1 (ko) 심장의 전구체 세포의 부분모집단 단리 방법 및 의료 분야에서의 관련 용도
CN1961069A (zh) 获得间充质干细胞的方法
CN103261407B (zh) 用于识别和培养具有高度增殖潜能的多能间充质干细胞的方法
JP2009060840A (ja) ヒト間葉系幹細胞濃縮方法
CN114317415A (zh) 人多能干细胞向多谱系大肠类器官定向分化的方法
Aboutaleb et al. A rapid and cost-effective protocol for isolating mesenchymal stem cells from the human amniotic membrane
Shen et al. Identification and functional study of immortalized mouse thymic epithelial cells
JP6786077B2 (ja) 胚様体の評価方法
HK40074847A (en) Mesenchymal stem cells
HK40074847B (zh) 一种间充质干细胞
EP3129469B1 (en) Cell-surface marker of early msc aging
JP5738538B2 (ja) 歯肉上皮由来の新規幹細胞およびその製造方法
US7638285B2 (en) Method of the discrimination and isolation of mammary epithelial stem and colony-forming cells
Liu et al. Isolation of Human BAMBIhighMFGE8high Umbilical Cord-Derived Mesenchymal Stromal Cells
Lopes et al. Isolation and characterization of mesenchymal stem cells from amniotic membrane
Moore et al. Bench-to-bedside optimization of mesenchymal stem cell isolation, processing, and expansion for in vivo administration
CN1088105C (zh) 血液携带的间质细胞

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40074847

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant