CA3163517C - HIGH-PURITY MESENCHYMAL STEM CELLS - Google Patents

HIGH-PURITY MESENCHYMAL STEM CELLS

Info

Publication number
CA3163517C
CA3163517C CA3163517A CA3163517A CA3163517C CA 3163517 C CA3163517 C CA 3163517C CA 3163517 A CA3163517 A CA 3163517A CA 3163517 A CA3163517 A CA 3163517A CA 3163517 C CA3163517 C CA 3163517C
Authority
CA
Canada
Prior art keywords
cell
cells
less
lngfr
mesenchymal stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3163517A
Other languages
English (en)
French (fr)
Other versions
CA3163517A1 (en
Inventor
Yumi Matsuzaki
Takashi Suyama
Original Assignee
Purec Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purec Co Ltd filed Critical Purec Co Ltd
Publication of CA3163517A1 publication Critical patent/CA3163517A1/en
Application granted granted Critical
Publication of CA3163517C publication Critical patent/CA3163517C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/10—Production naturally occurring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3163517A 2020-01-16 2020-04-06 HIGH-PURITY MESENCHYMAL STEM CELLS Active CA3163517C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/JP2020/002197 WO2021144995A1 (ja) 2020-01-16 2020-01-16 高純度間葉系幹細胞
JPPCT/JP2020/002197 2020-01-16
PCT/JP2020/016228 WO2021145002A1 (ja) 2020-01-16 2020-04-06 高純度間葉系幹細胞

Publications (2)

Publication Number Publication Date
CA3163517A1 CA3163517A1 (en) 2021-07-22
CA3163517C true CA3163517C (en) 2025-05-13

Family

ID=76864087

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3163517A Active CA3163517C (en) 2020-01-16 2020-04-06 HIGH-PURITY MESENCHYMAL STEM CELLS

Country Status (9)

Country Link
US (1) US20230002735A1 (https=)
EP (1) EP4053266A4 (https=)
JP (2) JP7466218B2 (https=)
KR (1) KR20220084078A (https=)
CN (1) CN114846134B (https=)
AU (1) AU2020423706B2 (https=)
CA (1) CA3163517C (https=)
IL (1) IL293724A (https=)
WO (2) WO2021144995A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022177032A1 (ja) * 2021-02-19 2022-08-25 ＰｕＲＥＣ株式会社 骨疾患治療用医薬組成物
AU2024354962A1 (en) 2023-10-02 2026-03-12 National University Corporation Tokai National Higher Education And Research System Method for managing quality of stem cells by image analysis using ai
TW202529782A (zh) * 2023-10-05 2025-08-01 日商ＰｕＲＥＣ股份有限公司 含有高純度間葉系幹細胞之用以治療以及／或是預防粒線體疾病之組成物

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6025329B2 (ja) 1980-07-25 1985-06-18 株式会社日立製作所 鋼管移送騒音防止装置
DE102006043625B4 (de) * 2006-09-12 2012-02-23 Eberhard-Karls-Universität Tübingen Universitätsklinikum Verfahren zur Isolierung und/oder Identifizierung von mesenchymalen Stammzellen (MSC)
JP2009060840A (ja) * 2007-09-06 2009-03-26 Keio Gijuku ヒト間葉系幹細胞濃縮方法
EP3124601A1 (en) * 2008-08-14 2017-02-01 Mesoblast International Sàrl Purified mesenchymal stem cell compositions and methods of purifying mesenchymal stem cell compositions
JP6463029B2 (ja) 2013-08-02 2019-01-30 有未 伊谷 ヒト間葉系幹細胞を特異的に認識するモノクローナル抗体並びにこれを用いたヒト間葉系幹細胞の分離及び／または品質評価を行う方法
WO2016017795A1 (ja) * 2014-08-01 2016-02-04 有未 伊谷 ヒト間葉系幹細胞の品質評価方法、及び、そのためのモノクローナル抗体
KR20160017795A (ko) 2014-08-05 2016-02-17 삼성디스플레이 주식회사 박막 트랜지스터 기판, 이의 제조 방법, 및 박막 트랜지스터 기판을 포함하는 표시 장치
EP3336177A1 (fr) * 2016-12-16 2018-06-20 Stem Cell Vet Therapeutics Procede de culture, d'isolement et d'enrichissement de cellules souches mesenchymateuses clonogeniques, a fort rendement, en vue d'une utilisation therapeutique

Also Published As

Publication number Publication date
CN114846134A (zh) 2022-08-02
WO2021145002A8 (ja) 2022-06-30
WO2021145002A1 (ja) 2021-07-22
JPWO2021145002A1 (https=) 2021-07-22
CN114846134B (zh) 2024-10-29
JP7466218B2 (ja) 2024-04-12
CA3163517A1 (en) 2021-07-22
AU2020423706A1 (en) 2022-06-23
IL293724A (en) 2022-08-01
US20230002735A1 (en) 2023-01-05
JP2024089675A (ja) 2024-07-03
WO2021144995A1 (ja) 2021-07-22
EP4053266A1 (en) 2022-09-07
EP4053266A4 (en) 2023-01-11
JP7792151B2 (ja) 2025-12-25
KR20220084078A (ko) 2022-06-21
AU2020423706B2 (en) 2024-10-24

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