CN114747417B - Efficient cutting seedling method for Huangjingguo - Google Patents

Efficient cutting seedling method for Huangjingguo Download PDF

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CN114747417B
CN114747417B CN202210435518.2A CN202210435518A CN114747417B CN 114747417 B CN114747417 B CN 114747417B CN 202210435518 A CN202210435518 A CN 202210435518A CN 114747417 B CN114747417 B CN 114747417B
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cutting
solution
seedbed
days
rooting
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CN114747417A (en
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阮宾
倪燕妹
余涛
陈海坚
周少新
郭振粤
袁克艳
许志文
胡达权
黎志勤
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GUANGDONG LAND RECLAMATION TROPICAL CROP SCIENCE RESEARCH INSTITUTE
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GUANGDONG LAND RECLAMATION TROPICAL CROP SCIENCE RESEARCH INSTITUTE
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

Abstract

The invention discloses a high-efficiency cutting seedling method for Huangjingguo, which comprises the following steps: deeply turning the seedbed, adding coconut husk and sand into the seedbed, uniformly mixing, adjusting the length, width and height of the seedbed, and finally, using a diluted broad-spectrum bactericide to soak the seedbed for later use; selecting yellow crystal seedlings which are vigorous in visible light and semi-lignified with leaves, then shearing branches, and shearing the branches into cutting slips with two leaves; immersing the cutting into the rooting agent solution A for 3-5min; dissolving naphthalene acetic acid and indolebutyric acid in ammonia water, adding water into the naphthalene acetic acid and indolebutyric acid, adding riboflavin and zeatin nucleoside, and uniformly mixing to obtain rooting agent solution A; cutting the cutting slips in a seedbed, then thoroughly watering with the solution B, and simultaneously building a small arch shed; spraying the mixed solution of the solution B and the broad-spectrum bactericide once every 2-4 days; spraying the mixed solution of the solution B and the solution C every 2-4 days after cutting for 30 days; and (5) after rooting for 10-20 days, uncovering the film for hardening off, and transplanting the seedlings into a field after rooting for 25-35 days.

Description

Efficient cutting seedling method for Huangjingguo
Technical Field
The invention relates to the field of a yellow crystal fruit seedling raising method, in particular to a high-efficiency cutting seedling raising method for yellow crystal fruits.
Background
At present, the propagation of the Huangjingguo mainly comprises seedlings and grafting, but the characteristics among individuals of the seedlings are separated, products with consistent quality cannot be obtained, the grafting is limited by time, and the Huangjingguo is usually grafted in spring and autumn, has long seedling raising period and is not beneficial to rapid propagation of high-quality seedlings.
The yellow crystal fruits have good taste, smooth and fresh sweetness and huge market potential, the selling price of the yellow crystal fruits can reach 100 yuan/kg at present, the seedlings of the high-quality yellow crystal fruits are in short supply, the cutting propagation can efficiently cultivate the high-quality seedlings of the yellow crystal fruits, and no relevant literature data report of the cutting of the yellow crystal fruits exists.
Disclosure of Invention
Based on the above, the invention aims to provide a high-efficiency cutting seedling method for the yellow fruit, so as to solve the problem of high-efficiency propagation of the yellow fruit, and the method has important significance for rapidly promoting excellent yellow fruit varieties and industrialized development thereof.
The invention relates to a high-efficiency cutting seedling method for Huangjingguo, which comprises the following steps:
deeply turning the seedbed, adding coconut husk and sand into the seedbed, uniformly mixing, adjusting the length, width and height of the seedbed, and finally, using a diluted broad-spectrum bactericide to soak the seedbed for standby, and waiting for cuttage;
selecting yellow crystal seedlings which are vigorous in visible light and semi-lignified with leaves, then shearing branches, shearing the branches into cutting slips with two leaves, enabling an upper shearing opening to be smooth, and enabling a lower shearing opening to be inclined;
immersing the cutting into the rooting agent solution A for 3-5min; dissolving naphthalene acetic acid and indolebutyric acid in ammonia water, adding water into the naphthalene acetic acid and indolebutyric acid, adding riboflavin and zeatin nucleoside, and uniformly mixing to obtain rooting agent solution A;
cutting the cutting slips in a seedbed, then thoroughly watering with the solution B, simultaneously building a small arch shed, covering a white film, and finally compacting the periphery of the film to ensure that the small arch shed is sealed and airtight; dissolving seaweed fertilizer in water to obtain a solution B;
spraying the mixed solution of the solution B and the broad-spectrum bactericide once every 2-4 days after cutting; spraying the mixed solution of the solution B and the solution C every 2-4 days after cutting for 30 days; wherein, the primer acetic acid is dissolved in 95 percent ethanol to obtain solution C;
after rooting, if the temperature in the greenhouse is too high, opening and ventilating the two ends of the film, avoiding the too high humidity temperature of the small arch greenhouse, preventing the cutting from being infected with diseases, after rooting for 10-20 days, uncovering the film for hardening seedlings, and after rooting for 25-35 days, transplanting the seedlings into a field.
In one implementation mode, the seedbed is deeply turned for 22-28cm, coco coir and sand with the mass ratio of 2:1 are added into the seedbed, after uniform mixing, the length of the seedbed is adjusted to be 2.5-3.5m, the width of the seedbed is adjusted to be 0.8-1.2m, the height of the seedbed is adjusted to be 15-20cm, and finally the seedbed is soaked in 1200-1800 times carbendazim solution for standby, and cutting is waited.
In one implementation mode, the technical scheme is that branches with the stem thickness of 3-7mm are cut, then the branches are cut into cutting slips with two leaves and the length of 6-9cm, the upper cutting opening is smooth, and the lower cutting opening is inclined at 45 degrees.
In one embodiment, 1-2cm of the cutting root is immersed in the rooting agent solution A for 3-5min.
In one implementation mode, cutting the cutting slips in a seedbed, wherein the cutting slips are cut at a cutting distance of 10-15cm and a cutting slip depth of 1-2cm, then pouring the cutting slips thoroughly by using the solution B, building a small arch shed, wherein the arch height is 50-90cm, the arch width is 80-120cm, coating a white film, and finally compacting the periphery of the film to enable the small arch shed to be sealed and airtight.
In an embodiment of the foregoing technical solution, the method for efficient cutting seedling raising of wampee fruits further includes: in order to avoid direct irradiation, a greenhouse with the arch height of 1.5-2.5m is built on the small arch greenhouse, and a shading net is covered, so that moisture evaporation of cutting shoots and seedbeds is reduced.
In one implementation mode, after cutting, spraying the mixed solution of the solution B and the carbendazim for 7-15 times every 2-4 days; after cutting for 30 days, spraying the mixed solution of the solution B and the solution C for 7-12 times every 2-4 days.
According to the technical scheme, in one implementation mode, after rooting, if the temperature in the greenhouse is too high, the two ends of the film are opened and ventilated, so that the situation that the humidity and the temperature of the small arch greenhouse are too high is avoided, the cutting is prevented from being infected with diseases, after rooting for 15 days, the film is uncovered for hardening seedlings, and after rooting for 30 days, the film can be transplanted into a field.
In one implementation mode, the technical scheme comprises 0.8-1.2mg/mL of naphthalene acetic acid, 0.8-1.2mg/mL of indolebutyric acid, 0.1-0.3mg/mL of riboflavin, 0.05-0.0.15mg/mL of zeatin nucleoside, 0.05-0.15mg/mL of indoleacetic acid and 0.1-0.3mg/mL of seaweed fertilizer.
In one implementation mode, 0.5g of naphthylacetic acid and 0.5g of indolebutyric acid are dissolved in 3mL of ammonia water with the concentration of 25%, then water is added into the naphthylacetic acid and the indolebutyric acid to obtain 500mL of mixed solution, namely 1mg/mL of naphthylacetic acid and 1mg/mL of indolebutyric acid are prepared, 0.1g of riboflavin and 0.05g of zeatin nucleoside are added into 500mL of mixed solution, and rooting agent solution A is obtained after uniform mixing;
dissolving 3g of seaweed fertilizer in 15kg of water to obtain a solution B;
after cutting, spraying the mixed solution of the solution B and the carbendazim once every 2-4 days, and mixing the solutions: 15kg of solution B are mixed with 15ml of carbendazim;
after cutting for 30 days, spraying the mixed solution of the solution B and the solution C every 2-4 days, wherein the mixing mode is as follows: adding the solution C into 15kg of the solution B to obtain a mixed solution; wherein, 1.5g of the indole acetic acid was dissolved in 5ml of 95% ethanol to obtain a solution C.
Compared with the prior art, the invention has the advantages that the interaction of the compounded rooting agent solution can enhance the metabolism of the yellow crystal fruit cutting, can obviously promote the rooting of the yellow crystal fruit cutting, and is particularly characterized in that the rooting rate is more than 95%; the corn cob base skin hole germination expansion and the formation of an internal root primordium can be effectively induced by adding the corn cob nucleoside; the riboflavin is added, so that the growth of cells can be effectively promoted, the healing of the basal part of the cutting slip is promoted, a large amount of callus is formed, and the survival rate is improved; meanwhile, due to the rooting effect of the indoleacetic acid and the nutrition effect of the seaweed fertilizer, the formation of adventitious roots and the growth of the roots of the cutting slips can be effectively promoted, so that the roots of the cutting slips are strong and developed, the plant resistance after cutting is good, the plant is not easy to suffer from diseases, and the seedling raising cost is low.
For a better understanding and implementation, the present invention is described in detail below with reference to the drawings.
Drawings
Fig. 1 is a schematic diagram of the efficient cutting seedling method of the wampee fruit.
Detailed Description
Terms of orientation such as up, down, left, right, front, rear, front, back, top, bottom, etc. mentioned or possible mentioned in this specification are defined with respect to their construction, and they are relative concepts. Therefore, the position and the use state of the device may be changed accordingly. These and other directional terms should not be construed as limiting terms.
The implementations described in the following exemplary examples are not representative of all implementations consistent with the present disclosure. Rather, they are merely examples of implementations consistent with aspects of the present disclosure.
The terminology used in the present disclosure is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. As used in this disclosure, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
Referring to the drawings, fig. 1 is a schematic diagram of the efficient cutting seedling method of the yellow fruit of the present invention.
The invention relates to a high-efficiency cutting seedling method for Huangjingguo, which is characterized by comprising the following steps of:
step 101, deep turning the seedbed, adding coconut coir and sand into the seedbed, uniformly mixing, adjusting the length, width and height of the seedbed, and finally, using a diluted broad-spectrum bactericide to soak the seedbed for standby, and waiting for cuttage.
In the concrete implementation, the seedbed is deeply turned for 22-28cm, then coconut husk and sand with the mass ratio of 2:1 are added into the seedbed, after uniform mixing, the seedbed is more loose and breathable and has proper humidity, cutting shoots are facilitated to root, the length of the seedbed is adjusted to be 2.5-3.5m, the width of the seedbed is 0.8-1.2m, the height of the seedbed is adjusted to be 15-20cm, and finally 1200-1800 times carbendazim solution is used for drenching the seedbed for standby, and cutting is waited for.
By setting the size of the seedbed, the management is facilitated, for example, the range of water showering of the shower is 0.4-0.6m when the shower is manually taken each time, and the shower is performed half at first and then the other half at the same time when the shower is conveniently operated each time; the small arched shed is convenient to construct, and the width of the arched shed formed when the arched shed is made is 1-1.2m because the arch bars used by the small arched shed are 2.2m in length, so that a seedbed with the width of 0.8-1.2m is selected. Furthermore, coconut coir is used: sand=2:1 is mixed with the deep-ploughed red loam, so that the seedbed is more loose and breathable, and rooting is facilitated. And the carbendazim solution is poured on the seedbed for sterilization treatment, so that the diseases, insects, germs and the like in the seedbed are killed, and the rot or non-survival of the base of the cutting due to the infection of the base of the cutting with the diseases, insects and the like is reduced.
Step 102, selecting yellow crystal seedlings which are vigorous in visible light and semi-lignified with leaves, then cutting branches, cutting the branches into cutting slips with two leaves, enabling an upper cutting opening to be smooth, and enabling a lower cutting opening to be inclined.
In the concrete implementation, the branches with the stem thickness of 3-7mm are cut, and then the branches are cut into cutting slips with two leaves and 6-9cm long, the upper cutting opening is smooth, and the lower cutting opening is a 45-degree inclined plane.
The diameter of the branches with the stem thickness of 3-7mm is proper, and the cutting heads with the specification have strong activity and vigorous activity. The lower shearing opening is designed to be 45-degree inclined plane, so that the base part of the cutting slip can be in contact with rooting agent solution in a large amount, the survival rate of the cutting slip is improved, and the cutting slip is convenient to cut; the upper cutting opening is smoothly arranged, so that the upper cutting opening is prevented from being too sharp, and the hand is prevented from being scratched in the cutting process; 2 leaves are reserved for each section of cutting, photosynthesis can be carried out, nutrition and nutrients can be provided, and survival of the cutting after cutting is facilitated.
Step 103, immersing the cutting slips in the rooting agent solution A for 3-5min; wherein, 0.5g of naphthalene acetic acid and 0.5g of indolebutyric acid are dissolved in 3mL of ammonia water with the concentration of 25%, then the naphthalene acetic acid and the indolebutyric acid are added with water to obtain 500mL of mixed solution, namely, 1mg/mL of naphthalene acetic acid and 1mg/mL of indolebutyric acid are prepared, 0.1g of riboflavin and 0.05g of zeatin nucleoside are added into 500mL of mixed solution, and the rooting agent solution A is obtained after uniform mixing.
In the concrete implementation, the cutting base part is immersed in the rooting agent solution A for 3-5min in a proportion of 1-2 cm. Therefore, the base part of the cutting slip is fully immersed in the rooting agent solution A, so that survival and rooting of the cutting slip after cutting are facilitated.
The zeatin nucleoside can strengthen physiological activities of the cutting slips, accelerate growth and differentiation of cut cells of the cutting slips, form a large number of root primordia, break through outwards, accelerate healing, promote formation of adventitious roots and grow and elongate. The riboflavin protects the basal part of the cutting pin from the influence of external bad environment, can provide partial auxiliary substances required by the formation of adventitious roots, can synthesize endogenous hormone of the cutting pin to promote callus, shortens rooting time and improves rooting rate of the cutting pin.
The formation of adventitious roots of the yellow pod cutting belongs to the rooting of calli, which mainly originate from cambium and vascular ray parenchyma cells. In the research, the main factors of the cutting rooting of the yellow fruits are calli, a large number of researches show that the zeatin nucleoside can promote the formation of the calli and the elongation growth of adventitious roots, and the researches show that the riboflavin can promote the formation of the adventitious roots. Therefore, the cutting is treated by the zeatin nucleoside and the riboflavin in combination with the rooting agent, so that the cutting of the yellow fruit is rapidly rooted and the propagation coefficient is enlarged.
104, cutting the cutting slips in a seedbed, and then thoroughly watering with a solution B, wherein the solution B can be directly absorbed and utilized by the cutting slips of the yellow crystal fruits, has quick absorption and remarkable fertilizer efficiency, can effectively provide nutrition and is beneficial to rooting of the cutting slips; sufficient moisture is also provided to keep the seedbed moist. Meanwhile, a small arch shed is built, a white film is covered, and finally the periphery of the film is compacted, so that the small arch shed is sealed and airtight; wherein, 3g of seaweed fertilizer is taken and dissolved in 15kg of water to obtain solution B.
In the concrete implementation, cutting the cutting slips in a seedbed, wherein the cutting slips are 10-15cm in distance and 1-2cm in depth, then pouring the cutting slips thoroughly by using the solution B, building a small arch shed, wherein the arch height is 50-90cm, the arch width is 80-120cm, coating a white film, and finally compacting the periphery of the film to ensure that the small arch shed is sealed and airtight.
Further, in order to avoid direct irradiation, a greenhouse with the arch height of 1.5-2.5m is built on the small arch greenhouse, and a shading net is covered, so that moisture evaporation of cutting shoots and seedbeds is reduced. The shading net can be 9 needles, and the light transmittance is 75%.
Step 105, spraying the mixed solution of the solution B and the broad-spectrum bactericide once every 2-4 days after cutting, and mixing the mixed solution: 15kg of solution B are mixed with 15ml of carbendazim; after cutting for 30 days, spraying the mixed solution of the solution B and the solution C every 2-4 days, wherein the mixing mode is as follows: adding the solution C into 15kg of the solution B to obtain a mixed solution; wherein, 1.5g of the indole acetic acid was dissolved in 5ml of 95% ethanol to obtain a solution C. .
The concentrations of the above raw materials used are respectively: naphthalene acetic acid 0.8-1.2mg/mL, indolebutyric acid 0.8-1.2mg/mL, riboflavin 0.1-0.3mg/mL, zeatin nucleoside 0.05-0.0.15mg/mL, indoleacetic acid 0.05-0.15mg/mL, and seaweed fertilizer 0.1-0.3mg/mL.
In the concrete implementation, after cutting, the mixed solution of the solution B and the carbendazim is sprayed once every 2-4 days for 7-15 times, which is favorable for providing nutrition and moisture, can effectively sterilize, prevent the base of the cutting slip from rotting and greatly improve the survival rate of the cutting slip; after cutting for 30 days, spraying the mixed solution of the solution B and the solution C for 7-12 times every 2-4 days, which is favorable for strengthening roots and forming a large number of adventitious roots and growing the roots, so that the roots are strong and developed, nutrients and moisture can be effectively provided, and the survival rate of cutting slips can be improved.
And 106, after rooting, if the temperature in the greenhouse is too high, opening and ventilating the two ends of the film, avoiding the too high humidity temperature of the small arch greenhouse, preventing the cutting from being infected by diseases, after rooting for 10-20 days, uncovering the film for hardening seedlings, and after rooting for 25-35 days, transplanting the film into a field.
To further demonstrate the practical effect of the efficient cutting seedling method of the present invention, analyses were performed in combination with the experimental conditions of the examples and 2 comparative examples provided below.
Examples:
step 201, deep turning the seedbed for 25cm, adding coconut husk and sand with the mass ratio of 2:1 into the seedbed, uniformly mixing, adjusting the length of the seedbed to be 3m, the width to be 1m and the height to be 18cm, and finally, using 1500 times carbendazim solution to soak the seedbed for standby, and waiting for cuttage.
Step 202, selecting yellow crystal seedlings with vigorous visible light and semi-lignified leaves, shearing branches with the stem thickness of 3-7mm, shearing the branches into cutting slips with two leaves and the length of 6-9cm, enabling an upper shearing opening to be smooth, and enabling a lower shearing opening to be 45-degree inclined plane.
Step 203, immersing the cutting base 1-2cm into the rooting agent solution A for 3-5min. Wherein, 0.5g of naphthalene acetic acid and 0.5g of indolebutyric acid are dissolved in 3mL of 25% ammonia water, then the naphthalene acetic acid and the indolebutyric acid are added with water to obtain 500mL of mixed solution, and then 0.1g of riboflavin and 0.05g of zeatin nucleoside are added into the 500mL of mixed solution, and the rooting agent solution A is obtained after uniform mixing.
And 204, cutting the cutting slips in a seedbed, wherein the cutting pitch is 12cm, the depth of the cutting slips is 1.5cm, then, pouring the cutting slips thoroughly by using the solution B, simultaneously building a small arch shed, wherein the arch height is 70cm, the arch width is 100cm, coating a white film, and finally compacting the periphery of the film to ensure that the small arch shed is sealed and airtight. Wherein, 3g of seaweed fertilizer is taken and dissolved in 15kg of water to obtain solution B.
Step 205, spraying the mixed solution of the solution B and the carbendazim once every 2-4 days after cutting, and continuously 10 times, wherein the mixed solution is mixed in the following way: 15kg of the solution B was added with the solution C to obtain a mixed solution. Spraying the mixed solution of the solution B and the solution C for 8 times every 2-4 days after cutting for 30 days; the mixing mode of the mixed solution is as follows: adding the solution C into 15kg of the solution B to obtain a mixed solution; wherein, 1.5g of the indole acetic acid was dissolved in 5ml of 95% ethanol to obtain a solution C.
And 206, after rooting, if the temperature in the greenhouse is too high, opening and ventilating the two ends of the film, avoiding the too high humidity temperature of the small arch greenhouse, preventing the cutting from being infected by diseases, uncovering the film after rooting for 15 days, hardening seedlings, and transplanting the seedlings into a field after rooting for 30 days.
Comparative example 1:
in the same manner as used in the example, but without adding 0.1g of riboflavin and 0.05g of zeatin nucleoside to solution A, the remainder were identical.
Comparative example 2:
the same cuttings are directly cut, and no treatment is performed as a control.
Examples and comparative example results:
yellow crystal fruit cutting data record table
TABLE 1 data recording table of yellow crystal heading
Analysis of results:
1. 15d after cutting in experimental examples, calli are formed on a large scale; the phloem of the basal part of the cutting pin begins to expand; the cuttage rooting rate is 95%, the average root number is 12 roots/plant, the average root length is 46.08mm, the longest root length is 63.37mm, the average root diameter is 2.96mm, and the maximum root diameter is 3.57mm.
Comparative example 1 calli were formed on a large scale 28 days after cutting; further separation at the cutting edge of the cutting edge base; the cuttage rooting rate is 65%, the average root number is 5 roots/plant, the average root length is 25.53mm, the longest root length is 39.32mm, the average root diameter is 2.32mm, and the maximum root diameter is 2.51mm.
Comparative example 2 after 50d cutting, most of the leaves of the cutting slips fall, only the cutting slips with baldness remain, and the base of the cutting slips are rotten, only a small part of cutting slip callus is formed, the average root length is 1 strip/root, the average root length is 12.59mm, and the average root diameter is 1.91mm. Comparative example 2 survival rate was 12%.
From the results, comparative example 2 shows very low rooting survival rate.
2. Although the rooting survival rate of the yellow fruits is effectively improved in comparative example 1, the formation of adventitious roots at the base of the cutting shoots is reduced because the riboflavin and the zeatin nucleoside are not added, the growth of the roots is greatly slowed down, and the roots still do not survive in a certain proportion; the cutting of the experimental example greatly improves the formation of adventitious roots and the survival rate of the cutting, and has long, strong and developed root systems.
3. The treatment effect is best by adding riboflavin and zeatin nucleoside, callus and adventitious roots can be formed rapidly, growth and elongation of the adventitious roots are promoted, the cuttage rooting rate is 95%, and the number of average root systems is 12 per plant. Therefore, under the synergistic effect of the riboflavin and the zeatin nucleoside, the formation of the cutting callus and the growth and elongation of adventitious roots of the cutting callus can be promoted.
At present, the cutting survival of the yellow fruits is not reported yet. The method provided by the invention can be used for rapidly propagating the yellow fruit by means of cuttage, solves the problem of supply and demand of seedlings, has important significance for sustainable development of the yellow fruit industry, and provides reference and reference for technical research on the aspect of cutting of the Bruguiera of the family Bruguiera.
Compared with the prior art, the invention has the advantages that the interaction of the compounded rooting agent solution can enhance the metabolism of the yellow crystal fruit cutting, can obviously promote the rooting of the yellow crystal fruit cutting, and is particularly characterized in that the rooting rate is more than 95%; the corn cob base skin hole germination expansion and the formation of an internal root primordium can be effectively induced by adding the corn cob nucleoside; the riboflavin is added, so that the growth of cells can be effectively promoted, the healing of the basal part of the cutting slip is promoted, a large amount of callus is formed, and the survival rate is improved; meanwhile, due to the rooting effect of the indoleacetic acid and the nutrition effect of the seaweed fertilizer, the formation of adventitious roots and the growth of the roots of the cutting slips can be effectively promoted, so that the roots of the cutting slips are strong and developed, the plant resistance after cutting is good, the plant is not easy to suffer from diseases, and the seedling raising cost is low.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims (4)

1. The efficient cutting seedling raising method for the wampee fruits is characterized by comprising the following steps of:
deeply turning 22-28cm of a seedbed, adding coconut husk and sand with the mass ratio of 2:1 into the seedbed, uniformly mixing, adjusting the length of the seedbed to be 2.5-3.5m, the width of the seedbed to be 0.8-1.2m and the height of the seedbed to be 15-20cm, and finally, using 1200-1800 times carbendazim solution to soak the seedbed for standby, and waiting for cuttage;
selecting yellow crystal seedlings which are vigorous in visible light and semi-lignified with leaves, then shearing branches, shearing the branches into cutting slips with two leaves, enabling an upper shearing opening to be smooth, and enabling a lower shearing opening to be inclined;
immersing the cutting into the rooting agent solution A for 3-5min; wherein, dissolving naphthalene acetic acid and indolebutyric acid in ammonia water, adding water into naphthalene acetic acid and indolebutyric acid, adding riboflavin and zeatin nucleoside, and uniformly mixing to obtain rooting agent solution A, wherein, 0.8-1.2mg/mL of naphthalene acetic acid, 0.8-1.2mg/mL of indolebutyric acid, 0.1-0.3mg/mL of riboflavin, 0.05-0.0.15mg/mL of zeatin nucleoside, 0.05-0.15mg/mL of indolebutyric acid and 0.1-0.3mg/mL of seaweed fertilizer;
cutting the cutting slips in a seedbed at a cutting distance of 10-15cm and a cutting depth of 1-2cm, then thoroughly watering with the solution B, simultaneously building a small arch shed with an arch height of 50-90cm and an arch width of 80-120cm, coating a white film, and finally compacting the periphery of the film to ensure that the small arch shed is sealed and airtight; dissolving seaweed fertilizer in water to obtain a solution B;
after cutting, spraying the mixed solution of the solution B and the carbendazim once every 2-4 days for 7-15 times; spraying the mixed solution of the solution B and the solution C for 7-12 times every 2-4 days after cutting for 30 days; wherein, the primer acetic acid is dissolved in 95 percent ethanol to obtain solution C;
after rooting, if the temperature in the greenhouse is too high, opening and ventilating the two ends of the film, avoiding the too high humidity temperature of the small arch greenhouse, preventing the cutting slip from being infected with diseases, uncovering the film for hardening seedlings after rooting for 15 days, and transplanting the seedlings into a field after rooting for 30 days;
in order to avoid direct irradiation, a greenhouse with the arch height of 1.5-2.5m is built on the small arch greenhouse, and a shading net is covered, so that moisture evaporation of cutting shoots and seedbeds is reduced.
2. The efficient cutting seedling method for the wampee fruits according to claim 1, wherein the method is characterized by comprising the following steps of: cutting the branches with the stem thickness of 3-7mm, cutting the branches into cutting slips with two leaves and the length of 6-9cm, and cutting the upper cutting edge smoothly and cutting the lower cutting edge by 45-degree inclined planes.
3. The efficient cutting seedling method for the wampee fruits according to claim 2, wherein the method is characterized by comprising the following steps of: immersing 1-2cm of the basal part of the cutting into the rooting agent solution A for 3-5min.
4. A method for efficient cutting seedling raising of wampee fruits according to any one of claims 1 to 3, wherein the method comprises the steps of: dissolving 0.5g of naphthalene acetic acid and 0.5g of indolebutyric acid in 3mL of ammonia water with the concentration of 25%, adding water into the naphthalene acetic acid and the indolebutyric acid to obtain 500mL of mixed solution, namely preparing 1mg/mL of naphthalene acetic acid and 1mg/mL of indolebutyric acid, adding 0.1g of riboflavin and 0.05g of zeatin nucleoside into the 500mL of mixed solution, and uniformly mixing to obtain rooting agent solution A;
dissolving 3g of seaweed fertilizer in 15kg of water to obtain a solution B;
after cutting, spraying the mixed solution of the solution B and the carbendazim once every 2-4 days, and mixing the solutions: 15kg of solution B are mixed with 15ml of carbendazim;
after cutting for 30 days, spraying the mixed solution of the solution B and the solution C every 2-4 days, wherein the mixing mode is as follows: adding the solution C into 15kg of the solution B to obtain a mixed solution; wherein, 1.5g of the indole acetic acid was dissolved in 5ml of 95% ethanol to obtain a solution C.
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